Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0014641

Physical Location & Seq

Genomic Coordinates Forward (+)

31360641 .. 31365315

Gene Structure 4675 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1311200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2409 bp

ATGGAAGCCCAAGTAGAGAAAGTGCATGTTGCACTTGGAAATGAGCTGCAAAATGGATTTAAGACCTTGGCGTGGATACTGAGGAGGTGGAAATCTCAGCCTATTTCCATAGTCATTCTCCATGTAACCTACAGCATTTCAGGGAAGGATTATGTTTACACACCATTTGGCAAGCTCCCTGCAAGTTCTGTAAGTGATGAGAAATTGAAGGTTCTTAGGAGGTATGAACAGAAAAAGATGGACAATATCCTTTCCAAGTATGTAGCGTTCTGCGGCAAGGTGAAAGCTGAAATACTTAAAGTGGAGCAAACTGATGAATCTATTCATAAGCTCATCCTAGACTTGATTTCGGGACTTCACATAACGAAATTGGTCATTGGATTTACATTCATCAAATCATCATCATGGAGAACGAAGACTGCAATCAGCGTGTTATTTTACATCCACGAGCACAAGACTGAGTCCTGCGAATTGTTCATAATTTGTGGGGGAAACGAGTTATGTCTAAGAGGAGAAAATGATCAAAGAATCCAGGTGGATGGGAAAGGAGTGATGGTTGCAAAAAAGAAAGACAAGGTCAGCTTCAAATTTATGTTAGAAAAGATGTTCCTTGAAAATCGTCACAGCCTGTTTGGTTCACCAACAAAAAAGGACTCGGCCAATTTGCAAAATTTGTGGGAAAATTGTGTTCAAGAAATGGAAATTTACTACCAGCAGTTGTGTTCAAATTTGGATGTAGAAAATTGTGACCAGAGAAATGGTACAGGTACTTCTCAGCAGCCTAGTCCAACAGAGGCTATAATGTTGCAAATTAATGGGAACCCTAATATGAGCCCTGCAGAAAAAATTGAGTTTCTTAGGAAAACAATAAGCGAAACTCGTGGGATGATAAGAATGAAGAAGAAAGAAGCCAAGGAAAATGCTGACAGGCATGCAAAAGCGGAACGGGCCATTTGTTTATGCGAGCGCCGGGCTCAGGAGTTAGACGTTCAAATTAAAGAAGAGGGTGACAATAGAAGAGAGTTGAACAAAGAATTAGACACGGAAAAAGAACAACTCCATGAAGTAGTTAACAACATTGAAGAAAGCTGGAACAAGATGAAGTCACTCATAGAGCTTCAATATGAGCTTTCACACAAGCTCCATATGTCCAAAATGGCAAAATCACAAGCGGAGGTCCAGCTAGAGAATGAAGTGATGAAACGTACAGACATGGAGAGAAAAATTGAAGTACTCCGACGAGAAAGAGATGTATTTCGACGAAGGATTGAATTTTGCAGACAACAGGATGCCACAGGAACGGCAGAAGGGTTGAGTGAATTGAGCTGCCGTCTCAGAGAGTACACAGATGAGGAGATCAAACAGGCCACCAACGAATTTTCAGAACGTTTTAGATTGAAATCAGGAAGTGATTTAAGCAGTGTCTATAAAGGGCGTATCAATCATGTCACAGTTGGTATCAAAGTTCTTAACTCGGCTAATGGACTGTCCCAAGAAGATTTTCAGGCTAAGGTGAAGCTAATCAGCAACATCAGACACCCACACCTGCTTGCCATGATGGGCTACTGCTTCCAGCCAAACTGCACTGTCTTTGAAGACATGCACAATGGTAGTTTGCAAGAAGTCTTATTTTCCTTCCATGAAAAAAAGAATCCGGCCCGGGACCTTCAGTGGCAGGACCGCATCCGCATTGCGGCAGAGGTTTGCTCTGCCTTATGCTACCTCCACATGGCTCAGCCTAAACCCATTATTCATGGCCACCTCAGCCCATCCAACGTCCTCCTCGACCACAATCTCGTGGCAAAGGTCAGTGGTTTAGGGCTCAACCAGTCCTACGACGAAAGTCATGTCGGATGGGACATACGGGCCCTTGGGATTTTGACTCTCAATCTTATAACTGGGAAGAACTGGGTTGATGAGGCTATGCTCATGGAAAAGGCAGGATTAGTTCGAGTTTTAGATGAGAAGGCTGGAGCATGGCCATTGGATTTGGCAGAAGAACTTGTGAGCTTGGTGTTAAGGTGTTTATCCGTTAACAATGGACCCAACACGGATTTAAGAATGGTGACAATAATGGAGGAACTTTGTGAAATCAGAAAAAAGGCCGATAGTTTAATAGCAAGAGGAGGATCTGGCTTGAATATTGAGGGAATTGTGCATGGAGAGGTCACCAATGAAGTCCCGAGGGTTTTCTTATGCCCCATATTTCAGGAAGTAATGAAGGATCCACATATAGCGGCTGATGGATTTTCGTATGAGAAAGAAGCCATTGGAGAATGGTTGCGACTGGGGCACGATACATCACCGATGACAAATTTGAAGCTTGAGCACAACACTGTAATCCCTAATCACACCTTACGGTCTCTCGCTCAAGATTGGCATTATAAAAGATCAATTCCATATCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr U-box

Protein Analysis

803

Amino Acids

91.77

Weight (kDa)

6.98

Isoelectric Point (pI)

48.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	473 - 613	6.1e-20	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	473 - 614	4e-09	Protein kinase domain
U-box	PF04564	727 - 797	2.4e-15	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014641)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g19540
malus_domestica	MD01G1040600.v1.1 MD15G1311200.v1.1
prunus_persica	Prupe.6G202800_v2.0.a1 Prupe.6G202800_v2.0.a1 Prupe.6G202800_v2.0.a1 Prupe.6G202800_v2.0.a1 Prupe.6G202800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0111051
rosa_laevigata	RLG00000017861
rosa_multiflora	Rmu_sc0003994.1_g000002
rosa_rugosa	Rorug02G0170700
rosa_samantha	Rh2AG223100 Rh2BG235100 Rh2CG226600 Rh2DG230900
rosa_wichuraiana	Rw2G017220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1895, 2385
AarI	CACCTGC	1 cut(s)	1554
Acc36I	ACCTGC	1 cut(s)	1554
AciI	CCGC	7 cut(s)	273, 941, 1172, 1681, 1687, 1694, 2237
AclI	AACGTT	1 cut(s)	1387
AclWI	GGATC	3 cut(s)	2137, 2219, 2232
AcoI	YGGCCR	3 cut(s)	657, 1756, 1979
AcsI	RAATTY	7 cut(s)	587, 670, 702, 727, 1271, 1376, 2314
AcuI	CTGAAG	1 cut(s)	1652
AfaI	GTAC	5 cut(s)	763, 769, 1207, 1233, 1343
AfiI	CCNNNNNNNGG	4 cut(s)	72, 976, 1693, 1729
AjnI	CCWGG	1 cut(s)	531
AjuI	GAANNNNNNNTTGG	2 cut(s)	1485, 1517
Alw21I	GWGCWC	2 cut(s)	453, 2331
Alw26I	GTCTC	2 cut(s)	1337, 2367
AlwI	GGATC	3 cut(s)	2137, 2219, 2232
Ama87I	CYCGRG	2 cut(s)	1659, 2182
AoxI	GGCC	8 cut(s)	657, 948, 1365, 1656, 1756, 1866, 1979, 2103
ApaI	GGGCCC	1 cut(s)	1870
ApeKI	GCWGC	3 cut(s)	46, 778, 1326
ApoI	RAATTY	7 cut(s)	587, 670, 702, 727, 1271, 1376, 2314
ArsI	GACNNNNNNTTYG	2 cut(s)	1833, 1865
AseI	ATTAAT	1 cut(s)	813
Asp700I	GAANNNNTTC	2 cut(s)	321, 1500
AspLEI	GCGC	1 cut(s)	969
AspS9I	GGNCC	8 cut(s)	948, 1177, 1657, 1663, 1678, 1866, 1867, 2042
AsuC2I	CCSGG	3 cut(s)	971, 1660, 1661
AsuHPI	GGTGA	7 cut(s)	292, 630, 1019, 1525, 2077, 2161, 2295
AvaI	CYCGRG	2 cut(s)	1659, 2182
AvaII	GGWCC	4 cut(s)	1177, 1663, 1678, 2042
BaeGI	GKGCMC	2 cut(s)	1870, 2295
BalI	TGGCCA	2 cut(s)	1758, 1981
BamHI	GGATCC	1 cut(s)	2224
BanII	GRGCYC	4 cut(s)	836, 976, 1824, 1870
BarI	GAAGNNNNNNTAC	2 cut(s)	754, 786
BauI	CACGAG	3 cut(s)	446, 879, 1796
BbsI	GAAGAC	2 cut(s)	422, 1602
Bbv12I	GWGCWC	2 cut(s)	453, 2331
BbvCI	CCTCAGC	1 cut(s)	1763
BbvI	GCAGC	3 cut(s)	33, 790, 1313
BccI	CCATC	7 cut(s)	232, 533, 547, 1552, 1777, 1848, 2237
BceAI	ACGGC	2 cut(s)	1314, 1317
BciT130I	CCWGG	1 cut(s)	533
BciVI	GTATCC	1 cut(s)	69
BclI	TGATCA	1 cut(s)	520
BcnI	CCSGG	3 cut(s)	971, 1660, 1661
BcoDI	GTCTC	2 cut(s)	1337, 2367
BfaI	CTAG	3 cut(s)	338, 783, 1184
BfmI	CTRYAG	2 cut(s)	130, 837
BfoI	RGCGCY	1 cut(s)	970
BfuAI	ACCTGC	1 cut(s)	1554
BfuI	GTATCC	1 cut(s)	69
BisI	GCNGC	6 cut(s)	47, 274, 779, 1327, 1695, 2238
BlpI	GCTNAGC	1 cut(s)	1734
BlsI	GCNGC	6 cut(s)	48, 275, 780, 1328, 1696, 2239
BmcAI	AGTACT	1 cut(s)	1233
Bme1390I	CCNGG	4 cut(s)	533, 971, 1660, 1661
Bme18I	GGWCC	4 cut(s)	1177, 1663, 1678, 2042
BmeT110I	CYCGRG	2 cut(s)	1659, 2182
BmgT120I	GGNCC	8 cut(s)	948, 1177, 1657, 1663, 1678, 1866, 1867, 2042
BmiI	GGNNCC	5 cut(s)	821, 1664, 1868, 2044, 2226
BmrFI	CCNGG	4 cut(s)	533, 971, 1660, 1661
BmrI	ACTGGG	3 cut(s)	1908, 1918, 2297
BmsI	GCATC	2 cut(s)	1279, 1692
BmuI	ACTGGG	3 cut(s)	1908, 1918, 2297
BoxI	GACNNNNGTC	1 cut(s)	1842
BpiI	GAAGAC	2 cut(s)	422, 1602
BplI	GAGNNNNNCTC	2 cut(s)	1691, 1723
BpmI	CTGGAG	1 cut(s)	1992
Bpu10I	CCTNAGC	3 cut(s)	975, 1509, 1763
Bpu1102I	GCTNAGC	1 cut(s)	1734
BpuEI	CTTGAG	2 cut(s)	2345, 2355
BpuMI	CCSGG	3 cut(s)	971, 1660, 1661
BsaBI	GATNNNNATC	1 cut(s)	1399
BsaI	GGTCTC	1 cut(s)	2367
BsaJI	CCNNGG	5 cut(s)	66, 912, 1659, 1870, 2183
BsaXI	ACNNNNNCTCC	4 cut(s)	540, 570, 1125, 1155
Bsc4I	CCNNNNNNNGG	4 cut(s)	72, 976, 1693, 1729
Bse1I	ACTGG	4 cut(s)	1828, 1903, 1913, 2292
Bse3DI	GCAATG	1 cut(s)	1689
Bse8I	GATNNNNATC	1 cut(s)	1399
BseBI	CCWGG	1 cut(s)	533
BseDI	CCNNGG	5 cut(s)	66, 912, 1659, 1870, 2183
BseGI	GGATG	9 cut(s)	333, 441, 544, 739, 891, 1294, 1683, 1769, 1859
BseJI	GATNNNNATC	1 cut(s)	1399
BseLI	CCNNNNNNNGG	4 cut(s)	72, 976, 1693, 1729
BseMI	GCAATG	1 cut(s)	1689
BseMII	CTCAG	8 cut(s)	71, 110, 450, 788, 989, 1348, 1748, 1777
BseNI	ACTGG	4 cut(s)	1828, 1903, 1913, 2292
BseRI	GAGGAG	5 cut(s)	97, 525, 1367, 1772, 2139
BseSI	GKGCMC	2 cut(s)	1870, 2295
BseXI	GCAGC	3 cut(s)	33, 790, 1313
BsgI	GTGCAG	1 cut(s)	1567
BshFI	GGCC	8 cut(s)	659, 950, 1367, 1658, 1758, 1868, 1981, 2105
BsiHKAI	GWGCWC	2 cut(s)	453, 2331
BsiHKCI	CYCGRG	2 cut(s)	1659, 2182
BsiSI	CCGG	3 cut(s)	970, 1655, 1660
BslFI	GGGAC	5 cut(s)	366, 1474, 1676, 1871, 2165
BslI	CCNNNNNNNGG	4 cut(s)	72, 976, 1693, 1729
BsmAI	GTCTC	2 cut(s)	1337, 2367
BsmBI	CGTCTC	1 cut(s)	1337
BsmFI	GGGAC	5 cut(s)	366, 1474, 1676, 1871, 2165
BsnI	GGCC	8 cut(s)	659, 950, 1367, 1658, 1758, 1868, 1981, 2105
Bso31I	GGTCTC	1 cut(s)	2367
BsoBI	CYCGRG	2 cut(s)	1659, 2182
Bsp120I	GGGCCC	1 cut(s)	1866
Bsp1286I	GDGCHC	7 cut(s)	453, 836, 976, 1824, 1870, 2295, 2331
Bsp143I	GATC	5 cut(s)	520, 1356, 2129, 2224, 2390
Bsp1720I	GCTNAGC	1 cut(s)	1734
BspACI	CCGC	7 cut(s)	273, 941, 1172, 1681, 1687, 1694, 2237
BspANI	GGCC	8 cut(s)	659, 950, 1367, 1658, 1758, 1868, 1981, 2105
BspCNI	CTCAG	8 cut(s)	72, 109, 451, 787, 988, 1347, 1747, 1776
BspLI	GGNNCC	5 cut(s)	821, 1664, 1868, 2044, 2226
BspMAI	CTGCAG	1 cut(s)	841
BspMI	ACCTGC	1 cut(s)	1554
BspPI	GGATC	3 cut(s)	2137, 2219, 2232
BspTNI	GGTCTC	1 cut(s)	2367
BsrDI	GCAATG	1 cut(s)	1689
BsrI	ACTGG	4 cut(s)	1828, 1903, 1913, 2292
BssECI	CCNNGG	5 cut(s)	66, 912, 1659, 1870, 2183
BssMI	GATC	5 cut(s)	520, 1356, 2129, 2224, 2390
BssSI	CACGAG	3 cut(s)	446, 879, 1796
BssT1I	CCWWGG	3 cut(s)	66, 912, 1870
Bst2BI	CACGAG	3 cut(s)	446, 879, 1796
Bst2UI	CCWGG	1 cut(s)	533
Bst4CI	ACNGT	5 cut(s)	1453, 1488, 1588, 2338, 2361
Bst6I	CTCTTC	2 cut(s)	996, 1012
BstC8I	GCNNGC	4 cut(s)	173, 933, 965, 1551
BstEII	GGTNACC	1 cut(s)	2167
BstF5I	GGATG	9 cut(s)	333, 441, 544, 739, 891, 1294, 1683, 1769, 1859
BstH2I	RGCGCY	1 cut(s)	970
BstHHI	GCGC	1 cut(s)	969
BstKTI	GATC	5 cut(s)	523, 1359, 2132, 2227, 2393
BstMAI	GTCTC	2 cut(s)	1337, 2367
BstMBI	GATC	5 cut(s)	520, 1356, 2129, 2224, 2390
BstMWI	GCNNNNNNNGC	3 cut(s)	947, 1764, 2290
BstNI	CCWGG	1 cut(s)	533
BstNSI	RCATGY	3 cut(s)	29, 935, 1603
BstPAI	GACNNNNGTC	1 cut(s)	1842
BstPI	GGTNACC	1 cut(s)	2167
BstSCI	CCNGG	4 cut(s)	531, 969, 1658, 1659
BstSFI	CTRYAG	2 cut(s)	130, 837
BstSLI	GKGCMC	2 cut(s)	1870, 2295
BstV1I	GCAGC	3 cut(s)	33, 790, 1313
BstV2I	GAAGAC	2 cut(s)	422, 1602
BstX2I	RGATCY	2 cut(s)	2129, 2224
BstXI	CCANNNNNNTGG	2 cut(s)	539, 758
BstYI	RGATCY	2 cut(s)	2129, 2224
BsuI	GTATCC	1 cut(s)	69
BsuRI	GGCC	8 cut(s)	659, 950, 1367, 1658, 1758, 1868, 1981, 2105
BtsCI	GGATG	9 cut(s)	333, 441, 544, 739, 891, 1294, 1683, 1769, 1859
BtsI	GCAGTG	1 cut(s)	1426
BtsIMutI	CAGTG	5 cut(s)	1426, 1584, 1676, 1816, 2334
BveI	ACCTGC	1 cut(s)	1554
Cac8I	GCNNGC	4 cut(s)	173, 933, 965, 1551
CfoI	GCGC	1 cut(s)	969
Cfr13I	GGNCC	8 cut(s)	948, 1177, 1657, 1663, 1678, 1866, 1867, 2042
Cfr9I	CCCGGG	1 cut(s)	1659
Csp6I	GTAC	5 cut(s)	762, 768, 1206, 1232, 1342
CspCI	CAANNNNNGTGG	2 cut(s)	656, 691
CviQI	GTAC	5 cut(s)	762, 768, 1206, 1232, 1342
DpnI	GATC	5 cut(s)	522, 1358, 2131, 2226, 2392
DpnII	GATC	5 cut(s)	520, 1356, 2129, 2224, 2390
EaeI	YGGCCR	3 cut(s)	657, 1756, 1979
Eam1104I	CTCTTC	2 cut(s)	996, 1012
EarI	CTCTTC	2 cut(s)	996, 1012
Eco130I	CCWWGG	3 cut(s)	66, 912, 1870
Eco24I	GRGCYC	4 cut(s)	836, 976, 1824, 1870
Eco31I	GGTCTC	1 cut(s)	2367
Eco47I	GGWCC	4 cut(s)	1177, 1663, 1678, 2042
Eco57I	CTGAAG	1 cut(s)	1652
Eco88I	CYCGRG	2 cut(s)	1659, 2182
Eco91I	GGTNACC	1 cut(s)	2167
EcoO109I	RGGNCCY	2 cut(s)	1663, 1867
EcoO65I	GGTNACC	1 cut(s)	2167
EcoRII	CCWGG	1 cut(s)	531
EcoT14I	CCWWGG	3 cut(s)	66, 912, 1870
EcoT38I	GRGCYC	4 cut(s)	836, 976, 1824, 1870
ErhI	CCWWGG	3 cut(s)	66, 912, 1870
Esp3I	CGTCTC	1 cut(s)	1337
FalI	AAGNNNNNCTT	2 cut(s)	566, 598
FaqI	GGGAC	5 cut(s)	366, 1474, 1676, 1871, 2165
FauNDI	CATATG	1 cut(s)	1146
FbaI	TGATCA	1 cut(s)	520
Fnu4HI	GCNGC	6 cut(s)	47, 274, 779, 1327, 1695, 2238
FokI	GGATG	9 cut(s)	320, 428, 551, 746, 898, 1301, 1670, 1756, 1866
FriOI	GRGCYC	4 cut(s)	836, 976, 1824, 1870
Fsp4HI	GCNGC	6 cut(s)	47, 274, 779, 1327, 1695, 2238
FspBI	CTAG	3 cut(s)	338, 783, 1184
GlaI	GCGC	1 cut(s)	968
GluI	GCNGC	6 cut(s)	47, 274, 779, 1327, 1695, 2238
GsuI	CTGGAG	1 cut(s)	1992
HaeII	RGCGCY	1 cut(s)	970
HaeIII	GGCC	8 cut(s)	659, 950, 1367, 1658, 1758, 1868, 1981, 2105
HapII	CCGG	3 cut(s)	970, 1655, 1660
HhaI	GCGC	1 cut(s)	969
Hin6I	GCGC	1 cut(s)	967
HinP1I	GCGC	1 cut(s)	967
HincII	GTYRAC	2 cut(s)	1072, 2035
HindII	GTYRAC	2 cut(s)	1072, 2035
HindIII	AAGCTT	1 cut(s)	2321
HinfI	GANTC	6 cut(s)	317, 461, 528, 653, 1651, 1882
HpaI	GTTAAC	2 cut(s)	1072, 2035
HpaII	CCGG	3 cut(s)	970, 1655, 1660
HphI	GGTGA	7 cut(s)	292, 630, 1019, 1525, 2077, 2161, 2295
Hpy166II	GTNNAC	5 cut(s)	157, 638, 1072, 1344, 2035
Hpy188I	TCNGA	6 cut(s)	1238, 1337, 1384, 1535, 1853, 2096
Hpy188III	TCNNGA	7 cut(s)	351, 692, 977, 1404, 2182, 2210, 2372
Hpy8I	GTNNAC	5 cut(s)	157, 638, 1072, 1344, 2035
Hpy99I	CGWCG	3 cut(s)	1242, 1263, 1841
HpyAV	CCTTC	8 cut(s)	139, 202, 1257, 1301, 1645, 1676, 1960, 2215
HpyCH4III	ACNGT	5 cut(s)	1453, 1488, 1588, 2338, 2361
HpyCH4IV	ACGT	4 cut(s)	987, 1204, 1387, 1776
HpyF10VI	GCNNNNNNNGC	3 cut(s)	947, 1764, 2290
HpySE526I	ACGT	4 cut(s)	987, 1204, 1387, 1776
HspAI	GCGC	1 cut(s)	967
Ksp22I	TGATCA	1 cut(s)	520
KspAI	GTTAAC	2 cut(s)	1072, 2035
Kzo9I	GATC	5 cut(s)	520, 1356, 2129, 2224, 2390
LmnI	GCTCC	4 cut(s)	180, 304, 1146, 1973
Lsp1109I	GCAGC	3 cut(s)	33, 790, 1313
LweI	GCATC	2 cut(s)	1279, 1692
MaeI	CTAG	3 cut(s)	338, 783, 1184
MaeII	ACGT	4 cut(s)	987, 1204, 1387, 1776
MaeIII	GTNAC	8 cut(s)	124, 620, 746, 1007, 1104, 1447, 2065, 2167
MalI	GATC	5 cut(s)	522, 1358, 2131, 2226, 2392
MboI	GATC	5 cut(s)	520, 1356, 2129, 2224, 2390
MflI	RGATCY	2 cut(s)	2129, 2224
MhlI	GDGCHC	7 cut(s)	453, 836, 976, 1824, 1870, 2295, 2331
MlsI	TGGCCA	2 cut(s)	1758, 1981
MluNI	TGGCCA	2 cut(s)	1758, 1981
MlyI	GAGTC	3 cut(s)	470, 647, 1876
MmeI	TCCRAC	4 cut(s)	812, 1261, 1797, 1831
Mox20I	TGGCCA	2 cut(s)	1758, 1981
MroXI	GAANNNNTTC	2 cut(s)	321, 1500
MscI	TGGCCA	2 cut(s)	1758, 1981
MslI	CAYNNNNRTG	2 cut(s)	403, 2404
Msp20I	TGGCCA	2 cut(s)	1758, 1981
MspI	CCGG	3 cut(s)	970, 1655, 1660
MspR9I	CCNGG	4 cut(s)	533, 971, 1660, 1661
MvaI	CCWGG	1 cut(s)	533
MwoI	GCNNNNNNNGC	3 cut(s)	947, 1764, 2290
NciI	CCSGG	3 cut(s)	971, 1660, 1661
NdeI	CATATG	1 cut(s)	1146
NdeII	GATC	5 cut(s)	520, 1356, 2129, 2224, 2390
NlaIV	GGNNCC	5 cut(s)	821, 1664, 1868, 2044, 2226
NmeAIII	GCCGAG	2 cut(s)	635, 1454
NmuCI	GTSAC	7 cut(s)	620, 746, 1007, 1104, 1447, 2065, 2167
NspI	RCATGY	3 cut(s)	29, 935, 1603
PaeI	GCATGC	1 cut(s)	935
PaqCI	CACCTGC	1 cut(s)	1554
PcsI	WCGNNNNNNNCGW	1 cut(s)	1782
PdmI	GAANNNNTTC	2 cut(s)	321, 1500
PfeI	GAWTC	3 cut(s)	317, 528, 1651
PflFI	GACNNNGTC	2 cut(s)	460, 575
PkrI	GCNGC	6 cut(s)	48, 275, 780, 1328, 1696, 2239
PleI	GAGTC	3 cut(s)	469, 647, 1876
PpsI	GAGTC	3 cut(s)	469, 647, 1876
PpuMI	RGGWCCY	1 cut(s)	1663
PshAI	GACNNNNGTC	1 cut(s)	1842
PshBI	ATTAAT	1 cut(s)	813
PsiI	TTATAA	2 cut(s)	1895, 2385
Psp1406I	AACGTT	1 cut(s)	1387
Psp5II	RGGWCCY	1 cut(s)	1663
Psp6I	CCWGG	1 cut(s)	531
PspEI	GGTNACC	1 cut(s)	2167
PspGI	CCWGG	1 cut(s)	531
PspN4I	GGNNCC	5 cut(s)	821, 1664, 1868, 2044, 2226
PspOMI	GGGCCC	1 cut(s)	1866
PspPI	GGNCC	8 cut(s)	948, 1177, 1657, 1663, 1678, 1866, 1867, 2042
PspPPI	RGGWCCY	1 cut(s)	1663
PsrI	GAACNNNNNNTAC	2 cut(s)	251, 283
PstI	CTGCAG	1 cut(s)	841
PsuI	RGATCY	2 cut(s)	2129, 2224
PsyI	GACNNNGTC	2 cut(s)	460, 575
RsaI	GTAC	5 cut(s)	763, 769, 1207, 1233, 1343
RsaNI	GTAC	5 cut(s)	762, 768, 1206, 1232, 1342
RseI	CAYNNNNRTG	2 cut(s)	403, 2404
SatI	GCNGC	6 cut(s)	47, 274, 779, 1327, 1695, 2238
Sau3AI	GATC	5 cut(s)	520, 1356, 2129, 2224, 2390
Sau96I	GGNCC	8 cut(s)	948, 1177, 1657, 1663, 1678, 1866, 1867, 2042
ScaI	AGTACT	1 cut(s)	1233
SchI	GAGTC	3 cut(s)	470, 647, 1876
ScrFI	CCNGG	4 cut(s)	533, 971, 1660, 1661
SduI	GDGCHC	7 cut(s)	453, 836, 976, 1824, 1870, 2295, 2331
SfaNI	GCATC	2 cut(s)	1279, 1692
SfcI	CTRYAG	2 cut(s)	130, 837
SinI	GGWCC	4 cut(s)	1177, 1663, 1678, 2042
SmaI	CCCGGG	1 cut(s)	1661
SmiMI	CAYNNNNRTG	2 cut(s)	403, 2404
SmlI	CTYRAG	2 cut(s)	2324, 2370
SmoI	CTYRAG	2 cut(s)	2324, 2370
SphI	GCATGC	1 cut(s)	935
SsiI	CCGC	7 cut(s)	273, 941, 1172, 1681, 1687, 1694, 2237
SspI	AATATT	1 cut(s)	2143
SspMI	CTAG	3 cut(s)	338, 783, 1184
StyD4I	CCNGG	4 cut(s)	531, 969, 1658, 1659
StyI	CCWWGG	3 cut(s)	66, 912, 1870
TaaI	ACNGT	5 cut(s)	1453, 1488, 1588, 2338, 2361
TaiI	ACGT	4 cut(s)	990, 1207, 1390, 1779
TaqI	TCGA	3 cut(s)	1258, 1785, 1951
TatI	WGTACW	2 cut(s)	1231, 1341
TauI	GCSGC	3 cut(s)	276, 1697, 2240
TfiI	GAWTC	3 cut(s)	317, 528, 1651
TscAI	CASTG	5 cut(s)	1426, 1591, 1676, 1816, 2341
TseFI	GTSAC	7 cut(s)	620, 746, 1007, 1104, 1447, 2065, 2167
TseI	GCWGC	3 cut(s)	46, 778, 1326
Tsp45I	GTSAC	7 cut(s)	620, 746, 1007, 1104, 1447, 2065, 2167
TspGWI	ACGGA	3 cut(s)	1058, 2020, 2066
TspMI	CCCGGG	1 cut(s)	1659
TspRI	CASTG	5 cut(s)	1426, 1591, 1676, 1816, 2341
Tth111I	GACNNNGTC	2 cut(s)	460, 575
VpaK11BI	GGWCC	4 cut(s)	1177, 1663, 1678, 2042
VspI	ATTAAT	1 cut(s)	813
XapI	RAATTY	7 cut(s)	587, 670, 702, 727, 1271, 1376, 2314
XceI	RCATGY	3 cut(s)	29, 935, 1603
XmaI	CCCGGG	1 cut(s)	1659
XmnI	GAANNNNTTC	2 cut(s)	321, 1500
XspI	CTAG	3 cut(s)	338, 783, 1184
ZrmI	AGTACT	1 cut(s)	1233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.