Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007191

Physical Location & Seq

Genomic Coordinates Reverse (-)

14544828 .. 14548497

Gene Structure 3670 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1045300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGCCTCATCACTGGAAATCCCTCTTCCTCCTCCTCCTACAACTCCTCAACCTCTGCTCCACCAAATCCCAGCCATTCACATCATCATCATCATCATCATCAAATCCCCACCTTCAATTCCCATGCAAGCCACCCCACCACAGCTCCTACCCTTTCTGCAACAACTCCCTCCCGATCCCCACCAGAGCTCAGTCCTTAATTTCCCTCCTTACCCTCCAAGAAAAGATCCAACAGCTCTCAAACAGCGCCTCTGCAATCCCCAGACTTGGCATCCCACCCTACGAATGGTGGTCCGAGTCCCTCCATGGCATAGCCACCAACGGCCCTGGTGTCTCTTTCAATGGCACCATCCCTTCAGCCACAAGCTTCCCACAAGTGATTGTCACAGCAGCTGCTTTCAACAGGACTCTCTGGTTCTCCGTCGGGGCAGCCATTGCTGTTGAGGCCAGAGCAATGTACAATTTTGGGCAAGCTGGGTTGACTTTTTGGGCACCCAATATCAATATCTTTAGGGACCCCAGATGGGGGAGGGGCCAGGAGACCCCTGGGGAGGACCCCATGGTTGCTTCTGCTTATGCTGTTGAGTTTGTGAAGGGTTTTCAGGGTGGGGGAATTAGAGATGGTGGGCTTGGAGCGAGGAGAGTGTTGGAAAGTGATGAGAGTGGTGATAGTTTAATGCTCTCTGCTTGTTGCAAGCATTTCACTGCTTATGATTTGGAGTCGTGGAGGAATTTCAGTAGATATAGCTTCAATGCTGTGGTTTCAGAACAAGATTTGGAGGATACATATCAGCCGCCTTTTCGAAGTTGTATACAACAAGGTAAAGCTAGCTGCTTAATGTGTTCTTACAATGCAGTAAATGGGGTTCCTGCTTGTGCACAGAAAGATCTCTTGGATAAGGCTCGGAACGAGTGGGGATTCAAAGGATATATCACCTCAGACTGTGATGCTGTGGCCACAGTGTTTGAATATCAGAACTACACAAAAAGTCCTGAGGACGCAGTTGCTGATGTTCTTAAAGCAGGGACGGATATTGATTGTGGCACGTACTTGCTTCGAAATACTCTATCTACAATCAAACAAGGGAAGGTGCAAGAGGAAGACATAGACAAGGCGCTTCTAAATCTTTTCTCTGTTCAACTCCGTCTTGGGTTATTTAATGGAGATCCCAGAAAAGGGAAATTTGGTAGTTTGGGAGCTCAGGATGTCTGTACCTCAGAGCATAAGACGTTGGCACTTGAGGCGGCAAGGCAGGGAATTGTGCTCCTAAAAAATGATAAGAAGTTTTTGCCTTTAAAAAGGGGTGTTGATTTTTCCTTGGCTGTCATTGGTCCATTGGCAAACAATTCAAGTTTGCTGGGTGGGGATTACACAGGAATTCCCTGCAACCCAAAGAGTCCTTTTGAGGGTCTTCAAGAGTACTCAACTAGAACATCTTATGTAGCTGGCTGCCAGGATGTACCATGTTTGTCTGATGCTGGGTTCGAAAAAGCAATTTATGCTGCAAAAAAAGCTGATTTTGTTGTTATAGTTGTTGGTATTGATGCATCACAAGAAAGAGAAGATCACGATCGAGTTAGTCTTCTCTTACCTGGTAAACAGATGGCCCTTGTATCTTCTGTGGCTGCTGCAAGCAAAGAACCAGTGATTCTAGTTCTTGCTGGCGGTGGACCACTTGATGTAACATTTGCCAAAGAAGATCTCCGAATTGCAAGCATTCTTTGGATTGGGTACCCTGGGGAAGCTGGCGGGAGAGCACTAGCAGAAGTCATCTTTGGAGATTTTAATCCAGGTGGAAGGCTGCCTATGACTTGGTATCCTGAGTCATTCACCAATGTACCAATGAATGATATGAACATGAGGGCTGATCCTTCTTTAGGTTACCCCGGAAGAACATACCGGTTTTACACTGGGGACAGGATATATGGATTCGGGCACGGGTTAAGCTATAGTAGTTTCACTTACAATATCGTCTCAGCGCCAAAAAAGTTAAGCTTATCAAAACCCTTCAAGGTAGATTTGAGTAGAAATGTACTACACCAACCAGGAGATGTACTTGACCATTTACACATTGATGAGGTAACGTCGTGTGAATCTTTGAGATTCTTGGTTGAAATCGCTGTGAAGAATAACGGAGACATGGATGGAGGCCATAGTGTGATGTTGTTCTCCAGAGTGCCAAAAGTTGTCAAGGGTGTTCCAGTACGACAGCTGATCGGATTTAACCGTGTGTATACGTTTTCAAACCAATATACTACAGCATCCATTCTAGTTGATCCCTGTACACATCTAAGCTTTGCAGATGAGCATGGGGAAAGGATATTGCCATTGGGTGAACATGTGTTAATGGTAGGAGATATACAACACTTTGTCTCGGTTGAAATTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009044 GO:0016020 GO:0016787 GO:0016798 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0097599 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

797

Amino Acids

87.02

Weight (kDa)

6.09

Isoelectric Point (pI)

35.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	118 - 374	1.7e-32	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	420 - 650	8.8e-47	Glycosyl hydrolase family 3 C-terminal domain
Fn3-like	PF14310	719 - 786	1.4e-06	Fibronectin type III-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10560
fragaria_vesca	FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940
malus_domestica	MD01G1045300.v1.1
prunus_persica	Prupe.6G210000_v2.0.a1 Prupe.6G210000_v2.0.a1
pyrus_communis	pycom01g07120
rosa_chinensis	RchiOBHm_Chr2g0109951
rosa_laevigata	RLG00000017773
rosa_multiflora	Rmu_sc0005173.1_g000029 Rmu_sc0028281.1_g000001 Rmu_sc0033728.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133850
rosa_rugosa	Rorug02G0164200
rosa_samantha	Rh2AG216100 Rh2BG226200 Rh2CG218300 Rh2DG221700
rosa_wichuraiana	Rw2G016690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1731
AccB1I	GGYRCC	3 cut(s)	344, 490, 1731
AccI	GTMKAC	2 cut(s)	811, 2234
AciI	CCGC	4 cut(s)	794, 1244, 1665, 1749
AclWI	GGATC	5 cut(s)	169, 220, 1160, 1862, 2270
AcoI	YGGCCR	1 cut(s)	954
AcsI	RAATTY	3 cut(s)	730, 1181, 1377
AcuI	CTGAAG	1 cut(s)	339
AflIII	ACRYGT	1 cut(s)	2338
AgeI	ACCGGT	1 cut(s)	1899
AjnI	CCWGG	8 cut(s)	325, 534, 544, 1452, 1591, 1735, 1789, 2044
AjuI	GAANNNNNNNTTGG	4 cut(s)	875, 907, 1758, 1790
Alw21I	GWGCWC	5 cut(s)	190, 880, 1201, 1266, 1759
Alw26I	GTCTC	5 cut(s)	337, 533, 1978, 2130, 2377
Alw44I	GTGCAC	1 cut(s)	876
AlwI	GGATC	5 cut(s)	169, 220, 1160, 1862, 2270
AlwNI	CAGNNNCTG	2 cut(s)	392, 1007
AoxI	GGCC	6 cut(s)	322, 444, 532, 954, 1605, 2149
ApaLI	GTGCAC	1 cut(s)	876
ApeKI	GCWGC	9 cut(s)	389, 392, 428, 831, 1449, 1502, 1625, 1628, 1801
ApoI	RAATTY	3 cut(s)	730, 1181, 1377
AseI	ATTAAT	1 cut(s)	2385
AsiGI	ACCGGT	1 cut(s)	1899
Asp718I	GGTACC	1 cut(s)	1731
AspLEI	GCGC	3 cut(s)	248, 1117, 1981
AspS9I	GGNCC	8 cut(s)	291, 323, 514, 532, 553, 1331, 1606, 1670
AsuC2I	CCSGG	1 cut(s)	1887
AsuHPI	GGTGA	4 cut(s)	677, 925, 1822, 2345
AsuII	TTCGAA	3 cut(s)	802, 1057, 1485
AsuNHI	GCTAGC	1 cut(s)	827
AvaII	GGWCC	5 cut(s)	291, 514, 553, 1331, 1670
AxyI	CCTNAGG	1 cut(s)	993
BaeGI	GKGCMC	3 cut(s)	493, 880, 1938
BaeI	ACNNNNGTAYC	2 cut(s)	1821, 1854
BalI	TGGCCA	1 cut(s)	956
BanI	GGYRCC	3 cut(s)	344, 490, 1731
BanII	GRGCYC	2 cut(s)	190, 1201
BbsI	GAAGAC	3 cut(s)	1107, 1403, 1574
Bbv12I	GWGCWC	5 cut(s)	190, 880, 1201, 1266, 1759
BbvI	GCAGC	9 cut(s)	379, 401, 440, 818, 1436, 1489, 1612, 1615, 1788
BccI	CCATC	5 cut(s)	356, 516, 614, 1597, 2138
BceAI	ACGGC	1 cut(s)	337
BciT130I	CCWGG	8 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 2046
BciVI	GTATCC	2 cut(s)	775, 1827
BcnI	CCSGG	1 cut(s)	1887
BcoDI	GTCTC	5 cut(s)	337, 533, 1978, 2130, 2377
BfaI	CTAG	5 cut(s)	828, 1428, 1652, 1760, 2270
BfmI	CTRYAG	2 cut(s)	1948, 2256
BfoI	RGCGCY	3 cut(s)	249, 1118, 1982
BfuI	GTATCC	2 cut(s)	775, 1827
BglII	AGATCT	2 cut(s)	886, 1699
BmcAI	AGTACT	1 cut(s)	1421
Bme1390I	CCNGG	9 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 1887, 2046
Bme18I	GGWCC	5 cut(s)	291, 514, 553, 1331, 1670
BmgT120I	GGNCC	8 cut(s)	291, 323, 514, 532, 553, 1331, 1606, 1670
BmiI	GGNNCC	8 cut(s)	346, 492, 515, 516, 533, 555, 867, 1733
BmrFI	CCNGG	9 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 1887, 2046
BmrI	ACTGGG	1 cut(s)	1920
BmsI	GCATC	6 cut(s)	279, 937, 1465, 1534, 1556, 2270
BmtI	GCTAGC	1 cut(s)	831
BmuI	ACTGGG	1 cut(s)	1920
BpiI	GAAGAC	3 cut(s)	1107, 1403, 1574
BpmI	CTGGAG	1 cut(s)	2155
Bpu10I	CCTNAGC	1 cut(s)	1200
Bpu14I	TTCGAA	3 cut(s)	802, 1057, 1485
BpuEI	CTTGAG	1 cut(s)	1259
BpuMI	CCSGG	1 cut(s)	1887
BsaAI	YACGTR	1 cut(s)	1047
BsaBI	GATNNNNATC	3 cut(s)	786, 1569, 1785
BsaI	GGTCTC	1 cut(s)	533
BsaJI	CCNNGG	9 cut(s)	304, 325, 544, 545, 558, 1317, 1735, 1736, 1885
BsaWI	WCCGGW	1 cut(s)	1899
BsaXI	ACNNNNNCTCC	4 cut(s)	41, 71, 128, 158
Bse118I	RCCGGY	1 cut(s)	1899
Bse1I	ACTGG	4 cut(s)	17, 1643, 1915, 2201
Bse21I	CCTNAGG	1 cut(s)	993
Bse3DI	GCAATG	2 cut(s)	432, 459
Bse8I	GATNNNNATC	3 cut(s)	786, 1569, 1785
BseBI	CCWGG	8 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 2046
BseDI	CCNNGG	9 cut(s)	304, 325, 544, 545, 558, 1317, 1735, 1736, 1885
BseGI	GGATG	6 cut(s)	270, 348, 1210, 1462, 2149, 2261
BseJI	GATNNNNATC	3 cut(s)	786, 1569, 1785
BseMI	GCAATG	2 cut(s)	432, 459
BseMII	CTCAG	7 cut(s)	203, 951, 984, 1214, 1230, 1812, 1989
BseNI	ACTGG	4 cut(s)	17, 1643, 1915, 2201
BseRI	GAGGAG	4 cut(s)	20, 23, 35, 652
BseSI	GKGCMC	3 cut(s)	493, 880, 1938
BseXI	GCAGC	9 cut(s)	379, 401, 440, 818, 1436, 1489, 1612, 1615, 1788
BseYI	CCCAGC	4 cut(s)	69, 473, 1357, 1478
Bsh1285I	CGRYCG	1 cut(s)	1573
BshFI	GGCC	6 cut(s)	324, 446, 534, 956, 1607, 2151
BshNI	GGYRCC	3 cut(s)	344, 490, 1731
BshTI	ACCGGT	1 cut(s)	1899
BsiEI	CGRYCG	1 cut(s)	1573
BsiHKAI	GWGCWC	5 cut(s)	190, 880, 1201, 1266, 1759
BsiSI	CCGG	2 cut(s)	1887, 1900
BslFI	GGGAC	4 cut(s)	283, 527, 1039, 1928
BsmAI	GTCTC	5 cut(s)	337, 533, 1978, 2130, 2377
BsmBI	CGTCTC	1 cut(s)	1978
BsmFI	GGGAC	4 cut(s)	283, 527, 1039, 1928
BsmI	GAATGC	1 cut(s)	1716
BsnI	GGCC	6 cut(s)	324, 446, 534, 956, 1607, 2151
Bso31I	GGTCTC	1 cut(s)	533
Bsp119I	TTCGAA	3 cut(s)	802, 1057, 1485
Bsp1286I	GDGCHC	7 cut(s)	190, 493, 880, 1201, 1266, 1759, 1938
Bsp1407I	TGTACA	2 cut(s)	456, 2282
Bsp19I	CCATGG	2 cut(s)	304, 558
BspACI	CCGC	4 cut(s)	794, 1244, 1665, 1749
BspANI	GGCC	6 cut(s)	324, 446, 534, 956, 1607, 2151
BspCNI	CTCAG	7 cut(s)	202, 950, 985, 1213, 1229, 1813, 1988
BspLI	GGNNCC	8 cut(s)	346, 492, 515, 516, 533, 555, 867, 1733
BspOI	GCTAGC	1 cut(s)	831
BspPI	GGATC	5 cut(s)	169, 220, 1160, 1862, 2270
BspT104I	TTCGAA	3 cut(s)	802, 1057, 1485
BspT107I	GGYRCC	3 cut(s)	344, 490, 1731
BspTNI	GGTCTC	1 cut(s)	533
BsrDI	GCAATG	2 cut(s)	432, 459
BsrFI	RCCGGY	1 cut(s)	1899
BsrGI	TGTACA	2 cut(s)	456, 2282
BsrI	ACTGG	4 cut(s)	17, 1643, 1915, 2201
BssAI	RCCGGY	1 cut(s)	1899
BssECI	CCNNGG	9 cut(s)	304, 325, 544, 545, 558, 1317, 1735, 1736, 1885
BssNAI	GTATAC	2 cut(s)	812, 2235
BssT1I	CCWWGG	3 cut(s)	304, 558, 1317
Bst1107I	GTATAC	2 cut(s)	812, 2235
Bst2UI	CCWGG	8 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 2046
Bst4CI	ACNGT	3 cut(s)	944, 961, 2228
Bst6I	CTCTTC	1 cut(s)	29
BstAPI	GCANNNNNTGC	2 cut(s)	434, 1499
BstAUI	TGTACA	2 cut(s)	456, 2282
BstBAI	YACGTR	1 cut(s)	1047
BstBI	TTCGAA	3 cut(s)	802, 1057, 1485
BstC8I	GCNNGC	9 cut(s)	128, 471, 695, 829, 1447, 1633, 1663, 1714, 1747
BstDEI	CTNAG	8 cut(s)	189, 937, 993, 1200, 1216, 1821, 1975, 2291
BstDSI	CCRYGG	2 cut(s)	304, 558
BstEII	GGTNACC	1 cut(s)	1880
BstENI	CCTNNNNNAGG	1 cut(s)	1875
BstF5I	GGATG	6 cut(s)	270, 348, 1210, 1462, 2149, 2261
BstH2I	RGCGCY	3 cut(s)	249, 1118, 1982
BstHHI	GCGC	3 cut(s)	248, 1117, 1981
BstMAI	GTCTC	5 cut(s)	337, 533, 1978, 2130, 2377
BstMCI	CGRYCG	1 cut(s)	1573
BstMWI	GCNNNNNNNGC	5 cut(s)	434, 443, 1241, 1499, 1511
BstNI	CCWGG	8 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 2046
BstNSI	RCATGY	1 cut(s)	2342
BstPI	GGTNACC	1 cut(s)	1880
BstSCI	CCNGG	9 cut(s)	325, 534, 544, 1452, 1591, 1735, 1789, 1885, 2044
BstSFI	CTRYAG	2 cut(s)	1948, 2256
BstSLI	GKGCMC	3 cut(s)	493, 880, 1938
BstV1I	GCAGC	9 cut(s)	379, 401, 440, 818, 1436, 1489, 1612, 1615, 1788
BstV2I	GAAGAC	3 cut(s)	1107, 1403, 1574
BstX2I	RGATCY	4 cut(s)	225, 886, 1165, 1699
BstYI	RGATCY	4 cut(s)	225, 886, 1165, 1699
BstZ17I	GTATAC	2 cut(s)	812, 2235
Bsu36I	CCTNAGG	1 cut(s)	993
BsuI	GTATCC	2 cut(s)	775, 1827
BsuRI	GGCC	6 cut(s)	324, 446, 534, 956, 1607, 2151
BtgI	CCRYGG	2 cut(s)	304, 558
BtsCI	GGATG	6 cut(s)	270, 348, 1210, 1462, 2149, 2261
BtsI	GCAGTG	1 cut(s)	702
BtsIMutI	CAGTG	5 cut(s)	10, 702, 966, 1650, 1908
Cac8I	GCNNGC	9 cut(s)	128, 471, 695, 829, 1447, 1633, 1663, 1714, 1747
CaiI	CAGNNNCTG	2 cut(s)	392, 1007
CfoI	GCGC	3 cut(s)	248, 1117, 1981
Cfr10I	RCCGGY	1 cut(s)	1899
Cfr13I	GGNCC	8 cut(s)	291, 323, 514, 532, 553, 1331, 1606, 1670
CseI	GACGC	1 cut(s)	1007
CsiI	ACCWGGT	1 cut(s)	1591
CspAI	ACCGGT	1 cut(s)	1899
CspCI	CAANNNNNGTGG	2 cut(s)	946, 981
CviAII	CATG	8 cut(s)	123, 305, 559, 1464, 1858, 2140, 2309, 2339
DdeI	CTNAG	8 cut(s)	189, 937, 993, 1200, 1216, 1821, 1975, 2291
DraI	TTTAAA	1 cut(s)	1296
EaeI	YGGCCR	1 cut(s)	954
Eam1104I	CTCTTC	1 cut(s)	29
EarI	CTCTTC	1 cut(s)	29
Ecl136II	GAGCTC	2 cut(s)	188, 1199
Eco130I	CCWWGG	3 cut(s)	304, 558, 1317
Eco24I	GRGCYC	2 cut(s)	190, 1201
Eco31I	GGTCTC	1 cut(s)	533
Eco47I	GGWCC	5 cut(s)	291, 514, 553, 1331, 1670
Eco53kI	GAGCTC	2 cut(s)	188, 1199
Eco57I	CTGAAG	1 cut(s)	339
Eco81I	CCTNAGG	1 cut(s)	993
Eco91I	GGTNACC	1 cut(s)	1880
EcoICRI	GAGCTC	2 cut(s)	188, 1199
EcoNI	CCTNNNNNAGG	1 cut(s)	1875
EcoO109I	RGGNCCY	2 cut(s)	514, 553
EcoO65I	GGTNACC	1 cut(s)	1880
EcoRI	GAATTC	1 cut(s)	1377
EcoRII	CCWGG	8 cut(s)	325, 534, 544, 1452, 1591, 1735, 1789, 2044
EcoT14I	CCWWGG	3 cut(s)	304, 558, 1317
EcoT22I	ATGCAT	1 cut(s)	1549
EcoT38I	GRGCYC	2 cut(s)	190, 1201
ErhI	CCWWGG	3 cut(s)	304, 558, 1317
Esp3I	CGTCTC	1 cut(s)	1978
FaeI	CATG	8 cut(s)	126, 308, 562, 1467, 1861, 2143, 2312, 2342
FalI	AAGNNNNNCTT	2 cut(s)	1979, 2011
FaqI	GGGAC	4 cut(s)	283, 527, 1039, 1928
FatI	CATG	8 cut(s)	122, 304, 558, 1463, 1857, 2139, 2308, 2338
FauI	CCCGC	1 cut(s)	1742
FblI	GTMKAC	2 cut(s)	811, 2234
FokI	GGATG	6 cut(s)	257, 335, 1217, 1469, 2156, 2248
FriOI	GRGCYC	2 cut(s)	190, 1201
FspBI	CTAG	5 cut(s)	828, 1428, 1652, 1760, 2270
GlaI	GCGC	3 cut(s)	247, 1116, 1980
GsaI	CCCAGC	4 cut(s)	73, 477, 1361, 1482
GsuI	CTGGAG	1 cut(s)	2155
HaeII	RGCGCY	3 cut(s)	249, 1118, 1982
HaeIII	GGCC	6 cut(s)	324, 446, 534, 956, 1607, 2151
HapII	CCGG	2 cut(s)	1887, 1900
HgaI	GACGC	1 cut(s)	1007
HhaI	GCGC	3 cut(s)	248, 1117, 1981
Hin1II	CATG	8 cut(s)	126, 308, 562, 1467, 1861, 2143, 2312, 2342
Hin6I	GCGC	3 cut(s)	246, 1115, 1979
HinP1I	GCGC	3 cut(s)	246, 1115, 1979
HincII	GTYRAC	1 cut(s)	480
HindII	GTYRAC	1 cut(s)	480
HindIII	AAGCTT	3 cut(s)	364, 1993, 2293
HpaII	CCGG	2 cut(s)	1887, 1900
HphI	GGTGA	4 cut(s)	677, 925, 1822, 2345
Hpy166II	GTNNAC	8 cut(s)	480, 812, 878, 1598, 1670, 2235, 2285, 2336
Hpy188I	TCNGA	9 cut(s)	295, 766, 906, 940, 975, 1219, 1474, 1706, 2219
Hpy188III	TCNNGA	7 cut(s)	172, 992, 1202, 1415, 1568, 1820, 2172
Hpy8I	GTNNAC	8 cut(s)	480, 812, 878, 1598, 1670, 2235, 2285, 2336
Hpy99I	CGWCG	2 cut(s)	425, 2089
HpyAV	CCTTC	7 cut(s)	122, 363, 586, 1081, 1791, 1881, 2017
HpyCH4III	ACNGT	3 cut(s)	944, 961, 2228
HpyCH4IV	ACGT	4 cut(s)	1046, 1229, 2084, 2237
HpyF10VI	GCNNNNNNNGC	5 cut(s)	434, 443, 1241, 1499, 1511
HpyF3I	CTNAG	8 cut(s)	189, 937, 993, 1200, 1216, 1821, 1975, 2291
HpySE526I	ACGT	4 cut(s)	1046, 1229, 2084, 2237
Hsp92II	CATG	8 cut(s)	126, 308, 562, 1467, 1861, 2143, 2312, 2342
HspAI	GCGC	3 cut(s)	246, 1115, 1979
KflI	GGGWCCC	1 cut(s)	514
KpnI	GGTACC	1 cut(s)	1735
LmnI	GCTCC	5 cut(s)	62, 149, 632, 1196, 1269
Lsp1109I	GCAGC	9 cut(s)	379, 401, 440, 818, 1436, 1489, 1612, 1615, 1788
LweI	GCATC	6 cut(s)	279, 937, 1465, 1534, 1556, 2270
MabI	ACCWGGT	1 cut(s)	1591
MaeI	CTAG	5 cut(s)	828, 1428, 1652, 1760, 2270
MaeII	ACGT	4 cut(s)	1046, 1229, 2084, 2237
MaeIII	GTNAC	4 cut(s)	382, 1681, 1880, 2080
MboII	GAAGA	9 cut(s)	16, 1112, 1403, 1574, 1574, 1608, 1709, 1902, 2137
MflI	RGATCY	4 cut(s)	225, 886, 1165, 1699
MhlI	GDGCHC	7 cut(s)	190, 493, 880, 1201, 1266, 1759, 1938
MlsI	TGGCCA	1 cut(s)	956
MluNI	TGGCCA	1 cut(s)	956
MlyI	GAGTC	5 cut(s)	305, 400, 728, 1405, 1832
MmeI	TCCRAC	2 cut(s)	253, 627
Mox20I	TGGCCA	1 cut(s)	956
Mph1103I	ATGCAT	1 cut(s)	1549
MscI	TGGCCA	1 cut(s)	956
MslI	CAYNNNNRTG	1 cut(s)	2073
Msp20I	TGGCCA	1 cut(s)	956
MspA1I	CMGCKG	2 cut(s)	392, 2212
MspI	CCGG	2 cut(s)	1887, 1900
MspR9I	CCNGG	9 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 1887, 2046
Mva1269I	GAATGC	1 cut(s)	1716
MvaI	CCWGG	8 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 2046
MwoI	GCNNNNNNNGC	5 cut(s)	434, 443, 1241, 1499, 1511
NciI	CCSGG	1 cut(s)	1887
NcoI	CCATGG	2 cut(s)	304, 558
NheI	GCTAGC	1 cut(s)	827
NlaIII	CATG	8 cut(s)	126, 308, 562, 1467, 1861, 2143, 2312, 2342
NlaIV	GGNNCC	8 cut(s)	346, 492, 515, 516, 533, 555, 867, 1733
NmuCI	GTSAC	1 cut(s)	382
NsiI	ATGCAT	1 cut(s)	1549
NspI	RCATGY	1 cut(s)	2342
NspV	TTCGAA	3 cut(s)	802, 1057, 1485
PacI	TTAATTAA	1 cut(s)	2389
PasI	CCCWGGG	2 cut(s)	545, 1736
PciI	ACATGT	1 cut(s)	2338
PctI	GAATGC	1 cut(s)	1716
PfeI	GAWTC	5 cut(s)	918, 1648, 1929, 2093, 2103
PinAI	ACCGGT	1 cut(s)	1899
Ple19I	CGATCG	1 cut(s)	1573
PleI	GAGTC	5 cut(s)	304, 400, 727, 1404, 1831
PpsI	GAGTC	5 cut(s)	304, 400, 727, 1404, 1831
Ppu21I	YACGTR	1 cut(s)	1047
PpuMI	RGGWCCY	2 cut(s)	514, 553
PscI	ACATGT	1 cut(s)	2338
PshBI	ATTAAT	1 cut(s)	2385
Psp124BI	GAGCTC	2 cut(s)	190, 1201
Psp5II	RGGWCCY	2 cut(s)	514, 553
Psp6I	CCWGG	8 cut(s)	325, 534, 544, 1452, 1591, 1735, 1789, 2044
PspEI	GGTNACC	1 cut(s)	1880
PspFI	CCCAGC	4 cut(s)	69, 473, 1357, 1478
PspGI	CCWGG	8 cut(s)	325, 534, 544, 1452, 1591, 1735, 1789, 2044
PspN4I	GGNNCC	8 cut(s)	346, 492, 515, 516, 533, 555, 867, 1733
PspPI	GGNCC	8 cut(s)	291, 323, 514, 532, 553, 1331, 1606, 1670
PspPPI	RGGWCCY	2 cut(s)	514, 553
PsrI	GAACNNNNNNTAC	2 cut(s)	849, 881
PstNI	CAGNNNCTG	2 cut(s)	392, 1007
PsuI	RGATCY	4 cut(s)	225, 886, 1165, 1699
PvuI	CGATCG	1 cut(s)	1573
PvuII	CAGCTG	2 cut(s)	392, 2212
RseI	CAYNNNNRTG	1 cut(s)	2073
SacI	GAGCTC	2 cut(s)	190, 1201
Sau96I	GGNCC	8 cut(s)	291, 323, 514, 532, 553, 1331, 1606, 1670
ScaI	AGTACT	1 cut(s)	1421
SchI	GAGTC	5 cut(s)	305, 400, 728, 1405, 1832
ScrFI	CCNGG	9 cut(s)	327, 536, 546, 1454, 1593, 1737, 1791, 1887, 2046
SduI	GDGCHC	7 cut(s)	190, 493, 880, 1201, 1266, 1759, 1938
SexAI	ACCWGGT	1 cut(s)	1591
SfaNI	GCATC	6 cut(s)	279, 937, 1465, 1534, 1556, 2270
SfcI	CTRYAG	2 cut(s)	1948, 2256
SfuI	TTCGAA	3 cut(s)	802, 1057, 1485
SinI	GGWCC	5 cut(s)	291, 514, 553, 1331, 1670
SmiMI	CAYNNNNRTG	1 cut(s)	2073
SmlI	CTYRAG	1 cut(s)	1238
SmoI	CTYRAG	1 cut(s)	1238
SsiI	CCGC	4 cut(s)	794, 1244, 1665, 1749
SspMI	CTAG	5 cut(s)	828, 1428, 1652, 1760, 2270
SstI	GAGCTC	2 cut(s)	190, 1201
StyD4I	CCNGG	9 cut(s)	325, 534, 544, 1452, 1591, 1735, 1789, 1885, 2044
StyI	CCWWGG	3 cut(s)	304, 558, 1317
TaaI	ACNGT	3 cut(s)	944, 961, 2228
TaiI	ACGT	4 cut(s)	1049, 1232, 2087, 2240
TaqI	TCGA	4 cut(s)	802, 1057, 1485, 1573
TatI	WGTACW	5 cut(s)	456, 1419, 2032, 2053, 2282
TauI	GCSGC	2 cut(s)	796, 1247
TfiI	GAWTC	5 cut(s)	918, 1648, 1929, 2093, 2103
TscAI	CASTG	5 cut(s)	17, 709, 966, 1650, 1915
TseFI	GTSAC	1 cut(s)	382
TseI	GCWGC	9 cut(s)	389, 392, 428, 831, 1449, 1502, 1625, 1628, 1801
Tsp45I	GTSAC	1 cut(s)	382
TspDTI	ATGAA	2 cut(s)	1859, 1868
TspGWI	ACGGA	4 cut(s)	409, 1043, 1133, 2148
TspRI	CASTG	5 cut(s)	17, 709, 966, 1650, 1915
VneI	GTGCAC	1 cut(s)	876
VpaK11BI	GGWCC	5 cut(s)	291, 514, 553, 1331, 1670
VspI	ATTAAT	1 cut(s)	2385
XagI	CCTNNNNNAGG	1 cut(s)	1875
XapI	RAATTY	3 cut(s)	730, 1181, 1377
XceI	RCATGY	1 cut(s)	2342
XcmI	CCANNNNNNNNNTGG	2 cut(s)	282, 542
XmiI	GTMKAC	2 cut(s)	811, 2234
XspI	CTAG	5 cut(s)	828, 1428, 1652, 1760, 2270
ZrmI	AGTACT	1 cut(s)	1421
Zsp2I	ATGCAT	1 cut(s)	1549

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.