Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0007191

Physical Location & Seq

Genomic Coordinates Reverse (-)

71318543 .. 71325978

Gene Structure 7436 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00133850.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3180 bp

ATGTCTCCTAATAACTGGAGATCATCCCTCTTCCTCCTCTTCCTCCTCCACTTCATCATATCATCATCATCATCAAATCCACACCCTCAGTTCCCATGCAACCAATCCCACCATAACACCCTGTACCCTTTTTGCAACACCTCCCTCTCCACCACCACCAGAGCCCAGTCCCTAATTTCCCTCCTCACCATCAATGAGAAAATCCAACAGCTCTCCAACACTGCCTCTGGAATCCCCAGACTTGGAATCCCACCCTATGAGTGGTGGTCTGAGTCACTCCATGGAATTGCCACAAATGGCCCTGGTGTCTCCTTCAATGGCACCATCCCTTCAGCCACAAGCTTCCCACAAGTGATTGTGACTGCATCTGCTTTCAATAGGACTCTGTGGAACTTGATTGGCTCTGCCATTGCTGTTGAGGCTAGAGCTATGTACAACTTTGGGCAAGCTGGGTTGACATTTTGGGCACCCAATATTAATATCTTTAGGGACCCAAGATGGGGGAGAGGCCAAGAGACCCCTGGGGAGGACCCCATGGTTGCTTCTGCTTATGCTGTTGAGTTTGTGAAGGGATTTCAAGGTGGGAATTGGGGAGGTGGGAGTGGTATTGGAAATGGGTTTGGAGGGAAAAGAGTGTTGGAAGAGAGTGATGGGTTAATGCTTTCAGCTTGTTGCAAGCATTTCACTGCTTATGATTTGGAGATGTGGGGAAAGTATAGTAGATATAGCTTCAATGCAGTGGTTTCAGAACAAGATTTGGAGGACACATATCAGCCGCCTTTTCGTAGCTGCATACAGCAAGGCAAAGCTAGCTGCTTGATGTGTTCTTACAATGCAGTAAATGGGGTTCCAGCATGTGCAGACAAGGATTTATTGGAGAAAGCTCGAGACAAATGGCGCTTCAAAGGGTATATCACCTCAGACTGTGATGCTGTCGCCACAGTTTTTGAATATCAGAATTATACAAAAACTGCTGAGGATGCAGTTGCTGATGTTCTTAAAGCAGGAATGGATATTAATTGTGGTACATTCTTGGTTCGAAATACTCTTTCTACAATCAAACAAGGAAAGGTGCAAGAGGAAGACATAGATAGGGCTCTTCTCAATCTTTTCTCCGTTCAACTCCGTCTTGGGCTGTTTGATGGAGATCCCATAAAAGGGCAATTTGGAAGGTTGGGACCTCAAGACGTGTGTGCATCAGCGCACAAAAATTTGGCACTCGAAGCAGCAAGGCAGGGAATTGTGCTTCTTAAAAATGATAAGAAGTTCCTGCCCCTGAAAAAGGGTGTTGATTTTTCGTTGGCTGTTATTGGTCCATTGGCGAACAATGCAAGTTTGCTGGGTGGGGGTTACACAGGGATTCCCTGCAACCCAAAGACTCCTTTTGAGGGTCTTCAGGAGTACAGAAAAAGAACATCTTACACAACTGGTTGCCTTGATGTACCATGTGTTTCTGATTCTGGATTCGAGGAAGCAATTCGTGCTGCCAAAAAAGCCGATTTTTCTGTTATAGTTGTTGGTCTTGATGCGTCACAAGAAAGAGAAGATCACGACCGAGTTAGTCTTCTCCTACCTGGTAAGCAGATGGCCCTTGTATCTTCAGTAGCTGCTGCAAGTAAAAACCCAGTGATTCTAGTTCTTGCTGGTGGTGGACCACTTGATGTATCATTCGCGGAAAAAGACCCGCGAATTGCTAGCATTCTTTGGATTGGGTACCCTGGTGAAACTGGTGGGAGGGCGCTATCAGAAATCATCTTTGGAGACGTTAATCCAAGTGGAAGGCTGCCCATGACTTGGTATTTTGAGTCATTCACCAACATCCCAATGAATGATATGAACATGAGGGCTGATCCTTCTCGTGGTTATCCAGGAAGAACCTACCGGTTTTACACAGGGAATAGGGTGTATGGATTTGGACATGGCTTGAGCTACACAAATTTCACATACAATATCGTGTCAGCACCAACCAAATTAAGCATACCAACACCTTTCAAGGTTGATTCAATCAAAAACATACTACACCTAGCAGGAGATATAGTTGATTATATACGCATAGATGAAGTGACATCTTGTGACTCCTTGAGATTTTCTGTCGAAATCAATGTGATGAATGTAGGAGACATGGATGGAAGCCATACTGTGATATTGTACTCTCGAGTGCCAGAAATTATGAAAGGTGCTCCAAAACAACAGCTGGTTGGATTTAATCAAGTGCATACTGTCTCATATAAATCTACATCAACAAGCATTTTGGTAGATCCTTGTACTCATCTTAGCTTTGCAAATGATTACGGCGAAAGGATACTGGCATTGGGTGATCATATAGGACTGCCTGCGGTAGTCAAATTAGCAGCAATCATGGCAGATGATCAGAATGATCTGAAGGAGGAGGTGGCGCTGGCAGACATTGCCCCTTTTGATCCTAGTAAAAAGAAGAAGAAGAAGAAGGTTGTAATTGAGGATCCAGCTGATGATTCTGTGGACAAGTTGGCTGAGAAAACGGAAAGCCTGGCAGGACAGTGCATTTTCTGGTCTGAAAAAAAGAAGAAGAAGAAGCCTATTGAAACAAGTATTTTGAATGAGGAGACTGGCGATGCACCGGAGGATTTGGATGAGCACCCAGGTGAGGATGAAGAAGGAGAAGGAATTTTACTGGAGGCCTCCAATCCTTGGGAGGGTAGTGATCGTGATTATGAATATGAGGAGCTTCTTGGAAGGGTGTATAACATTCTTAGGGAGAATAATCCGGAGCTAGCTGGAGACAGGCGTAGGACAGTTATGAGGCCTCCACAAGTTCTACGTGAGGGCACAAAGAAAACTGTCTTTGTGAATTTCATGGATCTGTGCAAGACGATGCATAGGCAACCAGAGCATGTCCAGAATTTCTTGTTGGCCGAACTGGGGACAAGTGGATCGCTTGATGGACAACAGAGGTTAGTTGTCAAGGGAAGGTTCGCCCCCAAGAATTTTGAAGGGATTCTGCGGCGATATGTCAGCTTGGTAGTTAATTATGTCTATGAATATGTGATTTGCCTTGGTTGCAAAAGCCCTGACACTATACTGTCAAAGGAGAATCGCCTGTATTTTCTCAGATGCGAGAAGTGTGGTTCTGGACGATCAGTTGCTCAGATCAAACAAGGTTTTGTTGCTCGTGTTGGGCGCAGGAATGCCGGGGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0009044 GO:0016020 GO:0016787 GO:0016798 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0097599 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Fn3-like Glyco_hydro_3 Glyco_hydro_3_C

Protein Analysis

1059

Amino Acids

116.67

Weight (kDa)

6.18

Isoelectric Point (pI)

35.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_3	PF00933	110 - 368	8.9e-31	Glycosyl hydrolase family 3 N terminal domain
Glyco_hydro_3_C	PF01915	414 - 644	4e-49	Glycosyl hydrolase family 3 C-terminal domain
eIF-5_eIF-2B	PF01873	921 - 1037	1.7e-34	Domain found in IF2B/IF5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007191)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10560
fragaria_vesca	FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940 FvH4_1g18940
malus_domestica	MD01G1045300.v1.1
prunus_persica	Prupe.6G210000_v2.0.a1 Prupe.6G210000_v2.0.a1
pyrus_communis	pycom01g07120
rosa_chinensis	RchiOBHm_Chr2g0109951
rosa_laevigata	RLG00000017773
rosa_multiflora	Rmu_sc0005173.1_g000029 Rmu_sc0028281.1_g000001 Rmu_sc0033728.1_g000001
rosa_roxburghii	Rroxscaffold_2G00133850
rosa_rugosa	Rorug02G0164200
rosa_samantha	Rh2AG216100 Rh2BG226200 Rh2CG218300 Rh2DG221700
rosa_wichuraiana	Rw2G016690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1713
AccB1I	GGYRCC	3 cut(s)	320, 466, 1713
AccB7I	CCANNNNNTGG	2 cut(s)	1794, 2670
AccII	CGCG	2 cut(s)	1673, 1687
AccIII	TCCGGA	1 cut(s)	2746
AciI	CCGC	5 cut(s)	776, 1673, 1685, 2336, 2983
AclWI	GGATC	8 cut(s)	1142, 1844, 2252, 2414, 2456, 2469, 2847, 2920
AcoI	YGGCCR	1 cut(s)	2892
AcsI	RAATTY	6 cut(s)	1210, 1936, 2646, 2830, 2881, 2965
AcuI	CTGAAG	4 cut(s)	315, 1379, 1584, 2402
AfaI	GTAC	8 cut(s)	125, 434, 1027, 1403, 1443, 1715, 2150, 2266
AflIII	ACRYGT	1 cut(s)	1188
AgeI	ACCGGT	1 cut(s)	1881
AjiI	CACGTC	1 cut(s)	1189
AjnI	CCWGG	7 cut(s)	301, 520, 1573, 1717, 1867, 2508, 2620
AjuI	GAANNNNNNNTTGG	6 cut(s)	620, 652, 1740, 1772, 2873, 2905
AleI	CACNNNNGTG	1 cut(s)	2622
Alw21I	GWGCWC	2 cut(s)	2182, 2619
Alw26I	GTCTC	9 cut(s)	9, 313, 509, 882, 1755, 2112, 2227, 2579, 2754
AlwI	GGATC	8 cut(s)	1142, 1844, 2252, 2414, 2456, 2469, 2847, 2920
AlwNI	CAGNNNCTG	2 cut(s)	989, 1607
Ama87I	CYCGRG	2 cut(s)	885, 2154
Aor13HI	TCCGGA	1 cut(s)	2746
AoxI	GGCC	6 cut(s)	298, 508, 1587, 2658, 2783, 2892
ApeKI	GCWGC	8 cut(s)	789, 813, 1226, 1484, 1607, 1610, 1783, 2351
ApoI	RAATTY	6 cut(s)	1210, 1936, 2646, 2830, 2881, 2965
AseI	ATTAAT	2 cut(s)	477, 1017
AsiGI	ACCGGT	1 cut(s)	1881
Asp700I	GAANNNNTTC	2 cut(s)	1476, 2976
Asp718I	GGTACC	1 cut(s)	1713
AspLEI	GCGC	5 cut(s)	900, 1204, 1741, 2398, 3162
AspS9I	GGNCC	7 cut(s)	299, 490, 529, 1178, 1313, 1588, 1652
AsuC2I	CCSGG	1 cut(s)	3172
AsuHPI	GGTGA	6 cut(s)	178, 907, 1733, 1804, 2327, 2636
AsuII	TTCGAA	1 cut(s)	1039
AsuNHI	GCTAGC	3 cut(s)	809, 1694, 2752
AvaI	CYCGRG	2 cut(s)	885, 2154
AvaII	GGWCC	5 cut(s)	490, 529, 1178, 1313, 1652
BaeGI	GKGCMC	2 cut(s)	469, 2810
BaeI	ACNNNNGTAYC	2 cut(s)	1705, 1738
BamHI	GGATCC	1 cut(s)	2461
BanI	GGYRCC	3 cut(s)	320, 466, 1713
BanII	GRGCYC	2 cut(s)	166, 1099
BarI	GAAGNNNNNNTAC	2 cut(s)	2634, 2666
BauI	CACGAG	2 cut(s)	1857, 3150
BbsI	GAAGAC	3 cut(s)	1089, 1385, 1556
Bbv12I	GWGCWC	2 cut(s)	2182, 2619
BbvCI	CCTCAGC	1 cut(s)	975
BbvI	GCAGC	8 cut(s)	776, 800, 1238, 1471, 1594, 1597, 1770, 2363
BccI	CCATC	8 cut(s)	197, 332, 492, 644, 1136, 1579, 2120, 2915
BceAI	ACGGC	1 cut(s)	2308
BciT130I	CCWGG	7 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622
BciVI	GTATCC	1 cut(s)	2295
BclI	TGATCA	2 cut(s)	2317, 2368
BcnI	CCSGG	1 cut(s)	3172
BcoDI	GTCTC	9 cut(s)	9, 313, 509, 882, 1755, 2112, 2227, 2579, 2754
BfaI	CTAG	7 cut(s)	423, 810, 1634, 1695, 2024, 2424, 2753
BfoI	RGCGCY	3 cut(s)	901, 1742, 2399
BfuI	GTATCC	1 cut(s)	2295
Bme1390I	CCNGG	8 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622, 3172
Bme18I	GGWCC	5 cut(s)	490, 529, 1178, 1313, 1652
BmeT110I	CYCGRG	2 cut(s)	885, 2154
BmgBI	CACGTC	1 cut(s)	1189
BmgT120I	GGNCC	7 cut(s)	299, 490, 529, 1178, 1313, 1588, 1652
BmiI	GGNNCC	9 cut(s)	322, 468, 491, 492, 531, 849, 1179, 1715, 2463
BmrFI	CCNGG	8 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622, 3172
BmrI	ACTGGG	3 cut(s)	160, 1619, 2909
BmsI	GCATC	8 cut(s)	374, 919, 970, 1205, 1516, 2584, 2844, 3083
BmtI	GCTAGC	3 cut(s)	813, 1698, 2756
BmuI	ACTGGG	3 cut(s)	160, 1619, 2909
BpiI	GAAGAC	3 cut(s)	1089, 1385, 1556
BpmI	CTGGAG	3 cut(s)	37, 2675, 2778
Bpu10I	CCTNAGC	1 cut(s)	975
Bpu14I	TTCGAA	1 cut(s)	1039
BpuEI	CTTGAG	3 cut(s)	1167, 1945, 2101
BpuMI	CCSGG	1 cut(s)	3172
BsaAI	YACGTR	1 cut(s)	2801
BsaBI	GATNNNNATC	1 cut(s)	1146
BsaI	GGTCTC	1 cut(s)	509
BsaWI	WCCGGW	3 cut(s)	1881, 2599, 2746
BsaXI	ACNNNNNCTCC	2 cut(s)	2022, 2052
Bse118I	RCCGGY	1 cut(s)	1881
Bse1I	ACTGG	9 cut(s)	20, 166, 1432, 1625, 1732, 2310, 2593, 2658, 2904
Bse3DI	GCAATG	2 cut(s)	408, 2406
Bse8I	GATNNNNATC	1 cut(s)	1146
BseAI	TCCGGA	1 cut(s)	2746
BseBI	CCWGG	7 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622
BseGI	GGATG	7 cut(s)	23, 324, 985, 1818, 2131, 2617, 2635
BseJI	GATNNNNATC	1 cut(s)	1146
BseMI	GCAATG	2 cut(s)	408, 2406
BseMII	CTCAG	7 cut(s)	101, 261, 933, 966, 2484, 3103, 3140
BseNI	ACTGG	9 cut(s)	20, 166, 1432, 1625, 1732, 2310, 2593, 2658, 2904
BseRI	GAGGAG	6 cut(s)	26, 35, 173, 2402, 2597, 2717
BseSI	GKGCMC	2 cut(s)	469, 2810
BseXI	GCAGC	8 cut(s)	776, 800, 1238, 1471, 1594, 1597, 1770, 2363
BseYI	CCCAGC	2 cut(s)	449, 1339
BsgI	GTGCAG	1 cut(s)	879
Bsh1236I	CGCG	2 cut(s)	1673, 1687
Bsh1285I	CGRYCG	1 cut(s)	1555
BshFI	GGCC	6 cut(s)	300, 510, 1589, 2660, 2785, 2894
BshNI	GGYRCC	3 cut(s)	320, 466, 1713
BshTI	ACCGGT	1 cut(s)	1881
BsiEI	CGRYCG	1 cut(s)	1555
BsiHKAI	GWGCWC	2 cut(s)	2182, 2619
BsiHKCI	CYCGRG	2 cut(s)	885, 2154
BsiSI	CCGG	4 cut(s)	1882, 2600, 2747, 3171
BslFI	GGGAC	4 cut(s)	154, 503, 1191, 2917
BsmAI	GTCTC	9 cut(s)	9, 313, 509, 882, 1755, 2112, 2227, 2579, 2754
BsmBI	CGTCTC	1 cut(s)	1755
BsmFI	GGGAC	4 cut(s)	154, 503, 1191, 2917
BsmI	GAATGC	2 cut(s)	1698, 3172
BsnI	GGCC	6 cut(s)	300, 510, 1589, 2660, 2785, 2894
Bso31I	GGTCTC	1 cut(s)	509
BsoBI	CYCGRG	2 cut(s)	885, 2154
Bsp119I	TTCGAA	1 cut(s)	1039
Bsp1286I	GDGCHC	6 cut(s)	166, 469, 1099, 2182, 2619, 2810
Bsp13I	TCCGGA	1 cut(s)	2746
Bsp1407I	TGTACA	1 cut(s)	432
Bsp19I	CCATGG	2 cut(s)	280, 534
BspACI	CCGC	5 cut(s)	776, 1673, 1685, 2336, 2983
BspANI	GGCC	6 cut(s)	300, 510, 1589, 2660, 2785, 2894
BspCNI	CTCAG	7 cut(s)	100, 262, 932, 967, 2485, 3102, 3139
BspEI	TCCGGA	1 cut(s)	2746
BspFNI	CGCG	2 cut(s)	1673, 1687
BspLI	GGNNCC	9 cut(s)	322, 468, 491, 492, 531, 849, 1179, 1715, 2463
BspOI	GCTAGC	3 cut(s)	813, 1698, 2756
BspPI	GGATC	8 cut(s)	1142, 1844, 2252, 2414, 2456, 2469, 2847, 2920
BspQI	GCTCTTC	1 cut(s)	1104
BspT104I	TTCGAA	1 cut(s)	1039
BspT107I	GGYRCC	3 cut(s)	320, 466, 1713
BspTNI	GGTCTC	1 cut(s)	509
BsrDI	GCAATG	2 cut(s)	408, 2406
BsrFI	RCCGGY	1 cut(s)	1881
BsrGI	TGTACA	1 cut(s)	432
BsrI	ACTGG	9 cut(s)	20, 166, 1432, 1625, 1732, 2310, 2593, 2658, 2904
BssAI	RCCGGY	1 cut(s)	1881
BssSI	CACGAG	2 cut(s)	1857, 3150
BssT1I	CCWWGG	4 cut(s)	280, 534, 2669, 3034
Bst2BI	CACGAG	2 cut(s)	1857, 3150
Bst2UI	CCWGG	7 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622
Bst4CI	ACNGT	8 cut(s)	926, 943, 2140, 2221, 2520, 2776, 2821, 3063
Bst6I	CTCTTC	4 cut(s)	35, 44, 636, 1104
BstAPI	GCANNNNNTGC	2 cut(s)	1481, 2408
BstAUI	TGTACA	1 cut(s)	432
BstBAI	YACGTR	1 cut(s)	2801
BstBI	TTCGAA	1 cut(s)	1039
BstC8I	GCNNGC	7 cut(s)	447, 677, 811, 1696, 2334, 2400, 2754
BstDEI	CTNAG	9 cut(s)	87, 270, 919, 975, 2273, 2493, 2732, 3089, 3126
BstDSI	CCRYGG	2 cut(s)	280, 534
BstENI	CCTNNNNNAGG	1 cut(s)	1280
BstF5I	GGATG	7 cut(s)	23, 324, 985, 1818, 2131, 2617, 2635
BstFNI	CGCG	2 cut(s)	1673, 1687
BstH2I	RGCGCY	3 cut(s)	901, 1742, 2399
BstHHI	GCGC	5 cut(s)	900, 1204, 1741, 2398, 3162
BstMAI	GTCTC	9 cut(s)	9, 313, 509, 882, 1755, 2112, 2227, 2579, 2754
BstMCI	CGRYCG	1 cut(s)	1555
BstNI	CCWGG	7 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622
BstNSI	RCATGY	2 cut(s)	858, 2876
BstSCI	CCNGG	8 cut(s)	301, 520, 1573, 1717, 1867, 2508, 2620, 3170
BstSLI	GKGCMC	2 cut(s)	469, 2810
BstUI	CGCG	2 cut(s)	1673, 1687
BstV1I	GCAGC	8 cut(s)	776, 800, 1238, 1471, 1594, 1597, 1770, 2363
BstV2I	GAAGAC	3 cut(s)	1089, 1385, 1556
BstX2I	RGATCY	4 cut(s)	1147, 2257, 2461, 2839
BstYI	RGATCY	4 cut(s)	1147, 2257, 2461, 2839
BsuI	GTATCC	1 cut(s)	2295
BsuRI	GGCC	6 cut(s)	300, 510, 1589, 2660, 2785, 2894
BtgI	CCRYGG	2 cut(s)	280, 534
BtgZI	GCGATG	1 cut(s)	2607
BtrI	CACGTC	1 cut(s)	1189
BtsCI	GGATG	7 cut(s)	23, 324, 985, 1818, 2131, 2617, 2635
BtsI	GCAGTG	3 cut(s)	219, 684, 744
BtsIMutI	CAGTG	5 cut(s)	219, 684, 744, 1632, 2525
Cac8I	GCNNGC	7 cut(s)	447, 677, 811, 1696, 2334, 2400, 2754
CaiI	CAGNNNCTG	2 cut(s)	989, 1607
CfoI	GCGC	5 cut(s)	900, 1204, 1741, 2398, 3162
Cfr10I	RCCGGY	1 cut(s)	1881
Cfr13I	GGNCC	7 cut(s)	299, 490, 529, 1178, 1313, 1588, 1652
CseI	GACGC	1 cut(s)	1518
CsiI	ACCWGGT	1 cut(s)	1573
Csp6I	GTAC	8 cut(s)	124, 433, 1026, 1402, 1442, 1714, 2149, 2265
CspAI	ACCGGT	1 cut(s)	1881
CspCI	CAANNNNNGTGG	2 cut(s)	928, 963
CviQI	GTAC	8 cut(s)	124, 433, 1026, 1402, 1442, 1714, 2149, 2265
DdeI	CTNAG	9 cut(s)	87, 270, 919, 975, 2273, 2493, 2732, 3089, 3126
EaeI	YGGCCR	1 cut(s)	2892
Eam1104I	CTCTTC	4 cut(s)	35, 44, 636, 1104
EarI	CTCTTC	4 cut(s)	35, 44, 636, 1104
Eco130I	CCWWGG	4 cut(s)	280, 534, 2669, 3034
Eco147I	AGGCCT	2 cut(s)	2660, 2785
Eco24I	GRGCYC	2 cut(s)	166, 1099
Eco31I	GGTCTC	1 cut(s)	509
Eco47I	GGWCC	5 cut(s)	490, 529, 1178, 1313, 1652
Eco57I	CTGAAG	4 cut(s)	315, 1379, 1584, 2402
Eco88I	CYCGRG	2 cut(s)	885, 2154
EcoNI	CCTNNNNNAGG	1 cut(s)	1280
EcoO109I	RGGNCCY	3 cut(s)	490, 529, 1178
EcoRII	CCWGG	7 cut(s)	301, 520, 1573, 1717, 1867, 2508, 2620
EcoT14I	CCWWGG	4 cut(s)	280, 534, 2669, 3034
EcoT22I	ATGCAT	1 cut(s)	2859
EcoT38I	GRGCYC	2 cut(s)	166, 1099
ErhI	CCWWGG	4 cut(s)	280, 534, 2669, 3034
Esp3I	CGTCTC	1 cut(s)	1755
FalI	AAGNNNNNCTT	2 cut(s)	1402, 1434
FaqI	GGGAC	4 cut(s)	154, 503, 1191, 2917
FauI	CCCGC	1 cut(s)	1692
FbaI	TGATCA	2 cut(s)	2317, 2368
FokI	GGATG	7 cut(s)	10, 311, 992, 1805, 2138, 2624, 2642
FriOI	GRGCYC	2 cut(s)	166, 1099
FspBI	CTAG	7 cut(s)	423, 810, 1634, 1695, 2024, 2424, 2753
GlaI	GCGC	5 cut(s)	899, 1203, 1740, 2397, 3161
GsaI	CCCAGC	2 cut(s)	453, 1343
GsuI	CTGGAG	3 cut(s)	37, 2675, 2778
HaeII	RGCGCY	3 cut(s)	901, 1742, 2399
HaeIII	GGCC	6 cut(s)	300, 510, 1589, 2660, 2785, 2894
HapII	CCGG	4 cut(s)	1882, 2600, 2747, 3171
HgaI	GACGC	1 cut(s)	1518
HhaI	GCGC	5 cut(s)	900, 1204, 1741, 2398, 3162
Hin6I	GCGC	5 cut(s)	898, 1202, 1739, 2396, 3160
HinP1I	GCGC	5 cut(s)	898, 1202, 1739, 2396, 3160
HincII	GTYRAC	1 cut(s)	456
HindII	GTYRAC	1 cut(s)	456
HindIII	AAGCTT	1 cut(s)	340
HpaII	CCGG	4 cut(s)	1882, 2600, 2747, 3171
HphI	GGTGA	6 cut(s)	178, 907, 1733, 1804, 2327, 2636
Hpy166II	GTNNAC	3 cut(s)	456, 1652, 2482
Hpy8I	GTNNAC	3 cut(s)	456, 1652, 2482
HpyCH4III	ACNGT	8 cut(s)	926, 943, 2140, 2221, 2520, 2776, 2821, 3063
HpyCH4IV	ACGT	3 cut(s)	1188, 1764, 2800
HpyF3I	CTNAG	9 cut(s)	87, 270, 919, 975, 2273, 2493, 2732, 3089, 3126
HpySE526I	ACGT	3 cut(s)	1188, 1764, 2800
HspAI	GCGC	5 cut(s)	898, 1202, 1739, 2396, 3160
KflI	GGGWCCC	1 cut(s)	490
Kpn2I	TCCGGA	1 cut(s)	2746
KpnI	GGTACC	1 cut(s)	1717
Ksp22I	TGATCA	2 cut(s)	2317, 2368
LguI	GCTCTTC	1 cut(s)	1104
LmnI	GCTCC	3 cut(s)	2185, 2704, 2749
Lsp1109I	GCAGC	8 cut(s)	776, 800, 1238, 1471, 1594, 1597, 1770, 2363
LweI	GCATC	8 cut(s)	374, 919, 970, 1205, 1516, 2584, 2844, 3083
MabI	ACCWGGT	1 cut(s)	1573
MaeI	CTAG	7 cut(s)	423, 810, 1634, 1695, 2024, 2424, 2753
MaeII	ACGT	3 cut(s)	1188, 1764, 2800
MaeIII	GTNAC	6 cut(s)	273, 358, 1349, 1530, 2062, 2072
MflI	RGATCY	4 cut(s)	1147, 2257, 2461, 2839
MhlI	GDGCHC	6 cut(s)	166, 469, 1099, 2182, 2619, 2810
MlyI	GAGTC	5 cut(s)	281, 376, 1372, 1814, 2069
MmeI	TCCRAC	4 cut(s)	229, 240, 618, 2179
Mph1103I	ATGCAT	1 cut(s)	2859
MroI	TCCGGA	1 cut(s)	2746
MroXI	GAANNNNTTC	2 cut(s)	1476, 2976
MseI	TTAA	9 cut(s)	477, 656, 999, 1017, 1251, 1767, 1973, 2205, 3006
MslI	CAYNNNNRTG	2 cut(s)	1823, 2622
MspA1I	CMGCKG	2 cut(s)	2194, 2468
MspI	CCGG	4 cut(s)	1882, 2600, 2747, 3171
MspR9I	CCNGG	8 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622, 3172
Mva1269I	GAATGC	2 cut(s)	1698, 3172
MvaI	CCWGG	7 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622
MvnI	CGCG	2 cut(s)	1673, 1687
NciI	CCSGG	1 cut(s)	3172
NcoI	CCATGG	2 cut(s)	280, 534
NheI	GCTAGC	3 cut(s)	809, 1694, 2752
NlaIV	GGNNCC	9 cut(s)	322, 468, 491, 492, 531, 849, 1179, 1715, 2463
NmuCI	GTSAC	5 cut(s)	273, 358, 1530, 2062, 2072
NsiI	ATGCAT	1 cut(s)	2859
NspI	RCATGY	2 cut(s)	858, 2876
NspV	TTCGAA	1 cut(s)	1039
OliI	CACNNNNGTG	1 cut(s)	2622
PaeR7I	CTCGAG	2 cut(s)	885, 2154
PasI	CCCWGGG	1 cut(s)	521
PceI	AGGCCT	2 cut(s)	2660, 2785
PciSI	GCTCTTC	1 cut(s)	1104
PctI	GAATGC	2 cut(s)	1698, 3172
PdmI	GAANNNNTTC	2 cut(s)	1476, 2976
PflMI	CCANNNNNTGG	2 cut(s)	1794, 2670
PfoI	TCCNGGA	1 cut(s)	1867
PinAI	ACCGGT	1 cut(s)	1881
PleI	GAGTC	5 cut(s)	280, 376, 1372, 1813, 2069
PpsI	GAGTC	5 cut(s)	280, 376, 1372, 1813, 2069
Ppu21I	YACGTR	1 cut(s)	2801
PpuMI	RGGWCCY	3 cut(s)	490, 529, 1178
PshBI	ATTAAT	2 cut(s)	477, 1017
Psp5II	RGGWCCY	3 cut(s)	490, 529, 1178
Psp6I	CCWGG	7 cut(s)	301, 520, 1573, 1717, 1867, 2508, 2620
PspFI	CCCAGC	2 cut(s)	449, 1339
PspGI	CCWGG	7 cut(s)	301, 520, 1573, 1717, 1867, 2508, 2620
PspN4I	GGNNCC	9 cut(s)	322, 468, 491, 492, 531, 849, 1179, 1715, 2463
PspPI	GGNCC	7 cut(s)	299, 490, 529, 1178, 1313, 1588, 1652
PspPPI	RGGWCCY	3 cut(s)	490, 529, 1178
PsrI	GAACNNNNNNTAC	2 cut(s)	831, 863
PstNI	CAGNNNCTG	2 cut(s)	989, 1607
PsuI	RGATCY	4 cut(s)	1147, 2257, 2461, 2839
PvuII	CAGCTG	2 cut(s)	2194, 2468
RsaI	GTAC	8 cut(s)	125, 434, 1027, 1403, 1443, 1715, 2150, 2266
RsaNI	GTAC	8 cut(s)	124, 433, 1026, 1402, 1442, 1714, 2149, 2265
RseI	CAYNNNNRTG	2 cut(s)	1823, 2622
SapI	GCTCTTC	1 cut(s)	1104
SaqAI	TTAA	9 cut(s)	477, 656, 999, 1017, 1251, 1767, 1973, 2205, 3006
Sau96I	GGNCC	7 cut(s)	299, 490, 529, 1178, 1313, 1588, 1652
SchI	GAGTC	5 cut(s)	281, 376, 1372, 1814, 2069
ScrFI	CCNGG	8 cut(s)	303, 522, 1575, 1719, 1869, 2510, 2622, 3172
SduI	GDGCHC	6 cut(s)	166, 469, 1099, 2182, 2619, 2810
SexAI	ACCWGGT	1 cut(s)	1573
SfaNI	GCATC	8 cut(s)	374, 919, 970, 1205, 1516, 2584, 2844, 3083
Sfr274I	CTCGAG	2 cut(s)	885, 2154
SfuI	TTCGAA	1 cut(s)	1039
SinI	GGWCC	5 cut(s)	490, 529, 1178, 1313, 1652
SlaI	CTCGAG	2 cut(s)	885, 2154
SmiMI	CAYNNNNRTG	2 cut(s)	1823, 2622
SmlI	CTYRAG	5 cut(s)	885, 1182, 1924, 2080, 2154
SmoI	CTYRAG	5 cut(s)	885, 1182, 1924, 2080, 2154
SseBI	AGGCCT	2 cut(s)	2660, 2785
SsiI	CCGC	5 cut(s)	776, 1673, 1685, 2336, 2983
SspI	AATATT	1 cut(s)	475
SspMI	CTAG	7 cut(s)	423, 810, 1634, 1695, 2024, 2424, 2753
StuI	AGGCCT	2 cut(s)	2660, 2785
StyD4I	CCNGG	8 cut(s)	301, 520, 1573, 1717, 1867, 2508, 2620, 3170
StyI	CCWWGG	4 cut(s)	280, 534, 2669, 3034
TaaI	ACNGT	8 cut(s)	926, 943, 2140, 2221, 2520, 2776, 2821, 3063
TaiI	ACGT	3 cut(s)	1191, 1767, 2803
TaqI	TCGA	6 cut(s)	886, 1039, 1221, 1467, 2094, 2155
TaqII	GACCGA	1 cut(s)	1569
TatI	WGTACW	4 cut(s)	432, 1401, 2148, 2264
TauI	GCSGC	2 cut(s)	778, 2986
Tru1I	TTAA	9 cut(s)	477, 656, 999, 1017, 1251, 1767, 1973, 2205, 3006
Tru9I	TTAA	9 cut(s)	477, 656, 999, 1017, 1251, 1767, 1973, 2205, 3006
TscAI	CASTG	5 cut(s)	226, 691, 744, 1632, 2525
TseFI	GTSAC	5 cut(s)	273, 358, 1530, 2062, 2072
TseI	GCWGC	8 cut(s)	789, 813, 1226, 1484, 1607, 1610, 1783, 2351
Tsp45I	GTSAC	5 cut(s)	273, 358, 1530, 2062, 2072
TspGWI	ACGGA	3 cut(s)	1105, 1115, 2516
TspRI	CASTG	5 cut(s)	226, 691, 744, 1632, 2525
Van91I	CCANNNNNTGG	2 cut(s)	1794, 2670
VpaK11BI	GGWCC	5 cut(s)	490, 529, 1178, 1313, 1652
VspI	ATTAAT	2 cut(s)	477, 1017
XagI	CCTNNNNNAGG	1 cut(s)	1280
XapI	RAATTY	6 cut(s)	1210, 1936, 2646, 2830, 2881, 2965
XceI	RCATGY	2 cut(s)	858, 2876
XcmI	CCANNNNNNNNNTGG	2 cut(s)	258, 518
XhoI	CTCGAG	2 cut(s)	885, 2154
XmnI	GAANNNNTTC	2 cut(s)	1476, 2976
XspI	CTAG	7 cut(s)	423, 810, 1634, 1695, 2024, 2424, 2753
Zsp2I	ATGCAT	1 cut(s)	2859

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.