MD01G1069100.v1.1

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005577
Physical Location & Seq
Reverse (-)
17313617 .. 17318232
4616 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1069100.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGGACTCGAGCTCTGCTTCAGCAGGTGACCACAACATCAGAGGGGTGCTGACCCACGGCGGACGCTACGTTCAGTACAATGTCTATGGCAACATCTTTGAAGTTTCCAGGAAGTACGTCCCGCCCCTTCGGCCCGTCGGCAGAGGTGCTTACGGTATCGTCTGTGCTGCTGTCAACGCTGAGGCTCGTGAGGAGGTTGCCATTAAGAAGATTGGTAATGCATTTGACAACAGAATAGATGCCAAAAGGACCTTACGAGAAATTAAACTTCTTCGGCACATGGATCATGAAAATGTTATTGCCATCAAAGACATCATACGGCCTCCACAGAAGGAGAACTTCAATGATGTCTACATTGTTTATGAATTAATGGACACTGATCTTCATCAGATTATACGGTCCAACCAACCTTTGAATGATGATCATTGTCGGTACTTTCTTTATCAGTTGTTACGTGGGCTCAAATATGTACATTCAGCTGGTGTTTTGCATCGTGATTTAAAGCCCAGCAATTTGTTCATGAATGCAAATTGTGACCTTAAGATTGGAGATTTTGGTCTTGCCAGGACAACATCTGAAACTGATTTCATGACTGAGTATGTTGTTACTCGTTGGTACCGTGCACCAGAATTGCTGCTTAATTGTTCGGAGTACACTGCAGCAATTGATATATGGTCTGTAGGTTGCATTCTAGGTGAAATCATGACCAGAAAACCCCTATTCCCTGGCAAAGATTATGTACATCAGCTGAGACTCATAACAGAGCTCTTAGGCTCACCTGATGACTCCAGCCTTGGATTTTTACGAAGTGATAATGCTCGAAGATATGTTCGACAACTACCTCAGTACCCAAAGCGGAGCTTCTCGCTCGGGTTTCCTAATATGTCTCCTAGCTCTATAGATTTGCTGGAGAAGATGCTTATCTTTGACCCAAACAGGCGCATTACAGTTGATGAGGCTCTTTCCCACCCATACTTGGCGCCTCTTCATGATATCAACGAGGAGCCTGTCTGCCCAATGCCTTTCAATTTTGATTTTGAGCAACCCTCGTTTACTGAAGAGAACATCAAGGAGCTCATCTGGAGAGAGTCCGTAAGGTTCAATCCAGATCCATTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006971 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009504 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009861 GO:0009862 GO:0009863 GO:0009867 GO:0009868 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0014070 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031122 GO:0032260 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032870 GO:0033206 GO:0033993 GO:0035556 GO:0035690 GO:0035821 GO:0036211 GO:0042221 GO:0042493 GO:0042539 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0046677 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051321 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052306 GO:0052308 GO:0052552 GO:0052553 GO:0052564 GO:0052572 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071840 GO:0075136 GO:0080026 GO:0097305 GO:0097435 GO:0098542 GO:0140013 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1901701 GO:1902410 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04016 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04138 ko04139 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05206 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 ko05418 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04016 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04138 map04139 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05206 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

374

Amino Acids

42.98

Weight (kDa)

6.14

Isoelectric Point (pI)

47.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 44 - 326 6.4e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 45 - 237 6.2e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 14
Acc36I ACCTGC 1 cut(s) 14
Acc65I GGTACC 1 cut(s) 615
AccB1I GGYRCC 2 cut(s) 615, 979
AccI GTMKAC 1 cut(s) 351
AciI CCGC 3 cut(s) 60, 122, 856
AclWI GGATC 2 cut(s) 291, 1103
AcuI CTGAAG 1 cut(s) 1077
AcyI GRCGYC 1 cut(s) 980
AfaI GTAC 8 cut(s) 77, 116, 434, 471, 617, 653, 741, 848
AfiI CCNNNNNNNGG 1 cut(s) 976
AflII CTTAAG 1 cut(s) 539
AgsI TTSAA 6 cut(s) 101, 343, 415, 1027, 1102, 1118
AjnI CCWGG 3 cut(s) 107, 563, 724
AloI GAACNNNNNNTCC 2 cut(s) 54, 86
AluBI AGCT 7 cut(s) 12, 479, 748, 766, 861, 894, 1075
AluI AGCT 7 cut(s) 12, 479, 748, 766, 861, 894, 1075
Alw21I GWGCWC 4 cut(s) 14, 625, 768, 1077
Alw26I GTCTC 2 cut(s) 745, 891
Alw44I GTGCAC 1 cut(s) 621
AlwI GGATC 2 cut(s) 291, 1103
Ama87I CYCGRG 2 cut(s) 7, 869
AoxI GGCC 2 cut(s) 131, 320
ApaLI GTGCAC 1 cut(s) 621
ApeKI GCWGC 3 cut(s) 167, 634, 659
AseI ATTAAT 1 cut(s) 368
Asp718I GGTACC 1 cut(s) 615
AspLEI GCGC 2 cut(s) 942, 982
AspS9I GGNCC 3 cut(s) 132, 249, 399
AsuHPI GGTGA 3 cut(s) 38, 707, 768
AvaI CYCGRG 2 cut(s) 7, 869
AvaII GGWCC 2 cut(s) 249, 399
BaeGI GKGCMC 1 cut(s) 625
BaeI ACNNNNGTAYC 2 cut(s) 830, 863
BanI GGYRCC 2 cut(s) 615, 979
BanII GRGCYC 4 cut(s) 14, 462, 768, 1077
BauI CACGAG 1 cut(s) 186
Bbv12I GWGCWC 4 cut(s) 14, 625, 768, 1077
BbvCI CCTCAGC 1 cut(s) 180
BbvI GCAGC 3 cut(s) 154, 621, 671
BccI CCATC 1 cut(s) 311
BceAI ACGGC 2 cut(s) 73, 335
BciT130I CCWGG 3 cut(s) 109, 565, 726
BclI TGATCA 1 cut(s) 421
BcoDI GTCTC 2 cut(s) 745, 891
BfaI CTAG 2 cut(s) 692, 891
BfmI CTRYAG 3 cut(s) 657, 678, 897
BfoI RGCGCY 1 cut(s) 983
BfrI CTTAAG 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 14
BglI GCCNNNNNGGC 1 cut(s) 130
BisI GCNGC 3 cut(s) 168, 635, 660
BlsI GCNGC 3 cut(s) 169, 636, 661
Bme1390I CCNGG 3 cut(s) 109, 565, 726
Bme18I GGWCC 2 cut(s) 249, 399
BmeT110I CYCGRG 2 cut(s) 7, 869
BmgT120I GGNCC 3 cut(s) 132, 249, 399
BmiI GGNNCC 3 cut(s) 617, 981, 1005
BmrFI CCNGG 3 cut(s) 109, 565, 726
BmsI GCATC 3 cut(s) 229, 499, 906
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
BpmI CTGGAG 3 cut(s) 772, 929, 1102
Bpu10I CCTNAGC 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 455
BsaBI GATNNNNATC 2 cut(s) 384, 920
BsaHI GRCGYC 1 cut(s) 980
BsaJI CCNNGG 3 cut(s) 55, 724, 793
Bsc4I CCNNNNNNNGG 1 cut(s) 976
Bse8I GATNNNNATC 2 cut(s) 384, 920
BseBI CCWGG 3 cut(s) 109, 565, 726
BseDI CCNNGG 3 cut(s) 55, 724, 793
BseJI GATNNNNATC 2 cut(s) 384, 920
BseLI CCNNNNNNNGG 1 cut(s) 976
BseMII CTCAG 4 cut(s) 171, 585, 740, 857
BseRI GAGGAG 2 cut(s) 206, 1016
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 3 cut(s) 154, 621, 671
BseYI CCCAGC 1 cut(s) 506
BshFI GGCC 2 cut(s) 133, 322
BshNI GGYRCC 2 cut(s) 615, 979
BsiHKAI GWGCWC 4 cut(s) 14, 625, 768, 1077
BsiHKCI CYCGRG 2 cut(s) 7, 869
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 976
BsmAI GTCTC 2 cut(s) 745, 891
BsmFI GGGAC 1 cut(s) 104
BsmI GAATGC 2 cut(s) 529, 687
BsnI GGCC 2 cut(s) 133, 322
BsoBI CYCGRG 2 cut(s) 7, 869
Bsp1286I GDGCHC 5 cut(s) 14, 462, 625, 768, 1077
Bsp1407I TGTACA 2 cut(s) 469, 739
Bsp143I GATC 4 cut(s) 283, 379, 421, 1108
BspACI CCGC 3 cut(s) 60, 122, 856
BspANI GGCC 2 cut(s) 133, 322
BspCNI CTCAG 4 cut(s) 172, 586, 741, 856
BspHI TCATGA 5 cut(s) 286, 519, 588, 702, 988
BspLI GGNNCC 3 cut(s) 617, 981, 1005
BspMAI CTGCAG 1 cut(s) 661
BspMI ACCTGC 1 cut(s) 14
BspPI GGATC 2 cut(s) 291, 1103
BspT107I GGYRCC 2 cut(s) 615, 979
BspTI CTTAAG 1 cut(s) 539
BsrGI TGTACA 2 cut(s) 469, 739
BssECI CCNNGG 3 cut(s) 55, 724, 793
BssMI GATC 4 cut(s) 283, 379, 421, 1108
BssNI GRCGYC 1 cut(s) 980
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 1 cut(s) 793
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 109, 565, 726
Bst4CI ACNGT 4 cut(s) 155, 399, 620, 949
Bst6I CTCTTC 2 cut(s) 990, 1053
BstACI GRCGYC 1 cut(s) 980
BstAFI CTTAAG 1 cut(s) 539
BstAUI TGTACA 2 cut(s) 469, 739
BstBAI YACGTR 1 cut(s) 455
BstDEI CTNAG 5 cut(s) 180, 594, 749, 769, 843
BstDSI CCRYGG 1 cut(s) 55
BstEII GGTNACC 1 cut(s) 26
BstH2I RGCGCY 1 cut(s) 983
BstHHI GCGC 2 cut(s) 942, 982
BstKTI GATC 4 cut(s) 286, 382, 424, 1111
BstMAI GTCTC 2 cut(s) 745, 891
BstMBI GATC 4 cut(s) 283, 379, 421, 1108
BstMWI GCNNNNNNNGC 2 cut(s) 130, 176
BstNI CCWGG 3 cut(s) 109, 565, 726
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 3 cut(s) 107, 563, 724
BstSFI CTRYAG 3 cut(s) 657, 678, 897
BstSLI GKGCMC 1 cut(s) 625
BstV1I GCAGC 3 cut(s) 154, 621, 671
BstX2I RGATCY 1 cut(s) 1108
BstYI RGATCY 1 cut(s) 1108
BsuRI GGCC 2 cut(s) 133, 322
BtgI CCRYGG 1 cut(s) 55
BtsI GCAGTG 1 cut(s) 654
BtsIMutI CAGTG 2 cut(s) 375, 654
BveI ACCTGC 1 cut(s) 14
CciI TCATGA 5 cut(s) 286, 519, 588, 702, 988
CfoI GCGC 2 cut(s) 942, 982
Cfr13I GGNCC 3 cut(s) 132, 249, 399
CseI GACGC 1 cut(s) 72
Csp6I GTAC 8 cut(s) 76, 115, 433, 470, 616, 652, 740, 847
CviAII CATG 6 cut(s) 280, 287, 520, 589, 703, 989
CviQI GTAC 8 cut(s) 76, 115, 433, 470, 616, 652, 740, 847
DdeI CTNAG 5 cut(s) 180, 594, 749, 769, 843
DinI GGCGCC 1 cut(s) 981
DpnI GATC 4 cut(s) 285, 381, 423, 1110
DpnII GATC 4 cut(s) 283, 379, 421, 1108
DraI TTTAAA 1 cut(s) 501
Eam1104I CTCTTC 2 cut(s) 990, 1053
EarI CTCTTC 2 cut(s) 990, 1053
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 3 cut(s) 12, 766, 1075
Eco130I CCWWGG 1 cut(s) 793
Eco24I GRGCYC 4 cut(s) 14, 462, 768, 1077
Eco32I GATATC 1 cut(s) 994
Eco47I GGWCC 2 cut(s) 249, 399
Eco53kI GAGCTC 3 cut(s) 12, 766, 1075
Eco57I CTGAAG 1 cut(s) 1077
Eco88I CYCGRG 2 cut(s) 7, 869
Eco91I GGTNACC 1 cut(s) 26
EcoICRI GAGCTC 3 cut(s) 12, 766, 1075
EcoO109I RGGNCCY 1 cut(s) 249
EcoO65I GGTNACC 1 cut(s) 26
EcoRII CCWGG 3 cut(s) 107, 563, 724
EcoRV GATATC 1 cut(s) 994
EcoT14I CCWWGG 1 cut(s) 793
EcoT22I ATGCAT 1 cut(s) 223
EcoT38I GRGCYC 4 cut(s) 14, 462, 768, 1077
EgeI GGCGCC 1 cut(s) 981
EheI GGCGCC 1 cut(s) 981
ErhI CCWWGG 1 cut(s) 793
FaeI CATG 6 cut(s) 283, 290, 523, 592, 706, 992
FalI AAGNNNNNCTT 4 cut(s) 323, 355, 845, 877
FaqI GGGAC 1 cut(s) 104
FatI CATG 6 cut(s) 279, 286, 519, 588, 702, 988
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 1 cut(s) 421
FblI GTMKAC 1 cut(s) 351
Fnu4HI GCNGC 3 cut(s) 168, 635, 660
FriOI GRGCYC 4 cut(s) 14, 462, 768, 1077
Fsp4HI GCNGC 3 cut(s) 168, 635, 660
FspBI CTAG 2 cut(s) 692, 891
GlaI GCGC 2 cut(s) 941, 981
GluI GCNGC 3 cut(s) 168, 635, 660
GsaI CCCAGC 1 cut(s) 510
GsuI CTGGAG 3 cut(s) 772, 929, 1102
HaeII RGCGCY 1 cut(s) 983
HaeIII GGCC 2 cut(s) 133, 322
HgaI GACGC 1 cut(s) 72
HhaI GCGC 2 cut(s) 942, 982
Hin1I GRCGYC 1 cut(s) 980
Hin1II CATG 6 cut(s) 283, 290, 523, 592, 706, 992
Hin6I GCGC 2 cut(s) 940, 980
HinP1I GCGC 2 cut(s) 940, 980
HincII GTYRAC 1 cut(s) 175
HindII GTYRAC 1 cut(s) 175
HinfI GANTC 4 cut(s) 5, 753, 785, 1088
HphI GGTGA 3 cut(s) 38, 707, 768
Hpy166II GTNNAC 5 cut(s) 175, 352, 623, 654, 1053
Hpy188I TCNGA 4 cut(s) 41, 390, 577, 649
Hpy188III TCNNGA 9 cut(s) 188, 287, 494, 520, 589, 703, 989, 1081, 1106
Hpy8I GTNNAC 5 cut(s) 175, 352, 623, 654, 1053
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 2 cut(s) 137, 325
HpyCH4III ACNGT 4 cut(s) 155, 399, 620, 949
HpyCH4IV ACGT 3 cut(s) 69, 117, 454
HpyCH4V TGCA 6 cut(s) 221, 490, 527, 623, 659, 687
HpyF10VI GCNNNNNNNGC 2 cut(s) 130, 176
HpyF3I CTNAG 5 cut(s) 180, 594, 749, 769, 843
HpySE526I ACGT 3 cut(s) 69, 117, 454
Hsp92I GRCGYC 1 cut(s) 980
Hsp92II CATG 6 cut(s) 283, 290, 523, 592, 706, 992
HspAI GCGC 2 cut(s) 940, 980
KasI GGCGCC 1 cut(s) 979
KpnI GGTACC 1 cut(s) 619
Ksp22I TGATCA 1 cut(s) 421
Kzo9I GATC 4 cut(s) 283, 379, 421, 1108
LmnI GCTCC 3 cut(s) 858, 1003, 1072
Lsp1109I GCAGC 3 cut(s) 154, 621, 671
LweI GCATC 3 cut(s) 229, 499, 906
MaeI CTAG 2 cut(s) 692, 891
MaeII ACGT 3 cut(s) 69, 117, 454
MaeIII GTNAC 4 cut(s) 26, 450, 533, 604
MalI GATC 4 cut(s) 285, 381, 423, 1110
MboI GATC 4 cut(s) 283, 379, 421, 1108
MboII GAAGA 7 cut(s) 220, 263, 374, 834, 925, 977, 1070
MfeI CAATTG 1 cut(s) 663
MflI RGATCY 1 cut(s) 1108
MhlI GDGCHC 5 cut(s) 14, 462, 625, 768, 1077
MluCI AATT 8 cut(s) 261, 365, 511, 529, 629, 640, 663, 1027
Mly113I GGCGCC 1 cut(s) 980
MlyI GAGTC 3 cut(s) 747, 779, 1097
MmeI TCCRAC 1 cut(s) 426
Mph1103I ATGCAT 1 cut(s) 223
MseI TTAA 6 cut(s) 204, 264, 368, 500, 540, 639
MslI CAYNNNNRTG 2 cut(s) 291, 1117
MspA1I CMGCKG 2 cut(s) 479, 748
MspCI CTTAAG 1 cut(s) 539
MspR9I CCNGG 3 cut(s) 109, 565, 726
MunI CAATTG 1 cut(s) 663
Mva1269I GAATGC 2 cut(s) 529, 687
MvaI CCWGG 3 cut(s) 109, 565, 726
MwoI GCNNNNNNNGC 2 cut(s) 130, 176
NarI GGCGCC 1 cut(s) 980
NdeII GATC 4 cut(s) 283, 379, 421, 1108
NlaIII CATG 6 cut(s) 283, 290, 523, 592, 706, 992
NlaIV GGNNCC 3 cut(s) 617, 981, 1005
NmuCI GTSAC 2 cut(s) 26, 533
NsiI ATGCAT 1 cut(s) 223
PaeR7I CTCGAG 1 cut(s) 7
PagI TCATGA 5 cut(s) 286, 519, 588, 702, 988
PaqCI CACCTGC 1 cut(s) 14
PcsI WCGNNNNNNNCGW 1 cut(s) 616
PctI GAATGC 2 cut(s) 529, 687
PfoI TCCNGGA 1 cut(s) 107
PkrI GCNGC 3 cut(s) 169, 636, 661
PleI GAGTC 3 cut(s) 747, 779, 1096
PluTI GGCGCC 1 cut(s) 983
PpsI GAGTC 3 cut(s) 747, 779, 1096
Ppu21I YACGTR 1 cut(s) 455
PpuMI RGGWCCY 1 cut(s) 249
PshBI ATTAAT 1 cut(s) 368
Psp124BI GAGCTC 3 cut(s) 14, 768, 1077
Psp5II RGGWCCY 1 cut(s) 249
Psp6I CCWGG 3 cut(s) 107, 563, 724
PspEI GGTNACC 1 cut(s) 26
PspFI CCCAGC 1 cut(s) 506
PspGI CCWGG 3 cut(s) 107, 563, 724
PspN4I GGNNCC 3 cut(s) 617, 981, 1005
PspPI GGNCC 3 cut(s) 132, 249, 399
PspPPI RGGWCCY 1 cut(s) 249
PspXI VCTCGAGB 1 cut(s) 7
PstI CTGCAG 1 cut(s) 661
PsuI RGATCY 1 cut(s) 1108
PvuII CAGCTG 2 cut(s) 479, 748
RsaI GTAC 8 cut(s) 77, 116, 434, 471, 617, 653, 741, 848
RsaNI GTAC 8 cut(s) 76, 115, 433, 470, 616, 652, 740, 847
RseI CAYNNNNRTG 2 cut(s) 291, 1117
SacI GAGCTC 3 cut(s) 14, 768, 1077
SaqAI TTAA 6 cut(s) 204, 264, 368, 500, 540, 639
SatI GCNGC 3 cut(s) 168, 635, 660
Sau3AI GATC 4 cut(s) 283, 379, 421, 1108
Sau96I GGNCC 3 cut(s) 132, 249, 399
SchI GAGTC 3 cut(s) 747, 779, 1097
ScrFI CCNGG 3 cut(s) 109, 565, 726
SduI GDGCHC 5 cut(s) 14, 462, 625, 768, 1077
SfaNI GCATC 3 cut(s) 229, 499, 906
SfcI CTRYAG 3 cut(s) 657, 678, 897
SfoI GGCGCC 1 cut(s) 981
Sfr274I CTCGAG 1 cut(s) 7
SinI GGWCC 2 cut(s) 249, 399
SlaI CTCGAG 1 cut(s) 7
SmiMI CAYNNNNRTG 2 cut(s) 291, 1117
SmlI CTYRAG 2 cut(s) 7, 539
SmoI CTYRAG 2 cut(s) 7, 539
Sse9I AATT 8 cut(s) 261, 365, 511, 529, 629, 640, 663, 1027
SsiI CCGC 3 cut(s) 60, 122, 856
SspDI GGCGCC 1 cut(s) 979
SspMI CTAG 2 cut(s) 692, 891
SstI GAGCTC 3 cut(s) 14, 768, 1077
StyD4I CCNGG 3 cut(s) 107, 563, 724
StyI CCWWGG 1 cut(s) 793
TaaI ACNGT 4 cut(s) 155, 399, 620, 949
TaiI ACGT 3 cut(s) 72, 120, 457
TaqI TCGA 3 cut(s) 8, 820, 832
TasI AATT 8 cut(s) 261, 365, 511, 529, 629, 640, 663, 1027
TatI WGTACW 4 cut(s) 75, 469, 651, 739
Tru1I TTAA 6 cut(s) 204, 264, 368, 500, 540, 639
Tru9I TTAA 6 cut(s) 204, 264, 368, 500, 540, 639
TscAI CASTG 2 cut(s) 382, 661
TseFI GTSAC 2 cut(s) 26, 533
TseI GCWGC 3 cut(s) 167, 634, 659
Tsp45I GTSAC 2 cut(s) 26, 533
TspDTI ATGAA 7 cut(s) 303, 374, 378, 508, 536, 577, 977
TspGWI ACGGA 1 cut(s) 1081
TspRI CASTG 2 cut(s) 382, 661
Vha464I CTTAAG 1 cut(s) 539
VneI GTGCAC 1 cut(s) 621
VpaK11BI GGWCC 2 cut(s) 249, 399
VspI ATTAAT 1 cut(s) 368
XhoI CTCGAG 1 cut(s) 7
XmiI GTMKAC 1 cut(s) 351
XspI CTAG 2 cut(s) 692, 891
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.