Rorug01G0254600

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0003642
Physical Location & Seq
Forward (+)
36380620 .. 36385503
4884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0254600.1

Sequence Viewer

Length: 2199 bp
ATGGCCGGTGGCGGAGGTGACGCGCCGCCTCTGCCGGCGTCTAACGGCGGCGGCGAATTCCTCCTCTCCTTGCTCCAGAAGGCCCCGCACCACCACTACCAGGGACGCTCGCCCCAACCACAGCAATCTACGGTGATCGATCCCGCCGTGGCCGCCGTGGGCCCCACCGTAACGTTCCAGCAGCAGATTCATCGGCCGTGGGGATCGTCGTCGTCATCCAACGGCCGAGATCATCCTCCTGCTCTTCACCACCACCGTCATCATCAGCAGATTCCGAACCCTTCCTGGCCCAATTCCCAATCTCCTCCTCCTCCTCCCTTCCTAGGGTTTCCTCAAAACCCCTACTCCTCTCCTCCCACGCCCAACCACTTCCCCCAGTTCGGCAGCAGCCAATTCCCCGGAAATCAAATCCTCGCCGACGATTTCAGGAAGATTGGGTTTTCGACTCCTCCGAACCTTGCTCTGCAGAAACAGCACCAAGAACCGAAGCTCAAGTTTAGTTATTTACCTGGCGATATTATCCGAAATCCGGAGTCTTCAGTAAATGCCATTACTTCCTCTGAAGTTGCCAAGCTCAGTAATGGCTTGGACCGCAATCTCCACTTGAATTCCAACAATTCATCTGAGTTTCGTCGCGGGAATTTCGATTCAGGGGAGCAAGAATCGAGAGGGGGAGGGGGCGGCGGCGGCGGCAGGGGGAAGCAAGTTCGTAGGACAATGCCGCCGCCGGGGTTCGGAAACAAGCCTAGGGGAGGAGGGAATTGGGATTCCGGGAGTAGGAGGGGAGGTATGGAGCACAATGTGGACAGGGAAAGGCAGAGTTCTAGTGAATTTGTGAGGAATAGGGATGCTTCTATTGAGAATGAGAGAGTGAAAAGACTGGGAGGTGAAGATGGTCGAATTCGAGGAAATGGGGCTAGTCGAACGGGGCTCAGTGCACAGCTTGATCGCCCTGGTCCACCCGCTGGCACCAATCTCCATTCTGTTTCTGCTTCGGAGATTGAAGAGTCGATGATGAATTTCGAGGATGGGGAGAGGGCAAGAAAGGATAGTGGCGGCGGTGAAGATGTAAGCCAACACTTGCTTGAGGATGAAACGGATGACAAGAATGAGGCAAAGCAACACCATAAGGATTCGAGATCAGATAACAGAGGACAACACCAACTTAGCCAAAGAGCGAGATCGTACAAAAGACAAACACTATGTCGCTTTGACATAGACAGACTAAATGCTCCTTTTCTTGAAATTTTTGAATCCTTGATACCAACTGAGGAAGAAAAGGGCAAGCAGAAGCAATTATTGACATTGTTGGAGAATATAATTTGCAAAGAATGGCCTGAAGCTCGCCTTTATATTTATGGATCATGTGGCAATTCATTTGGAGTTTCTAAAAGTGATATAGATCTCTGTCTGGTGATTGGTGAAGAAGATATTAACAAATCTGAGATCTTACTACGGTTGGCAGACCTTTTGCAGTCAGACAATCTAGAGAATGTACAGGCATTGACGCGTGCTAGGGTCCCCATAGTAAAGCTTATGGATCCTGTCACTGGAATATCTTGTGATATATGCATCAACAATGTTCTGGCTGTGGTAAATACAAAGCTTCTTCGTGATTATGCAAATATAGATGCAAGATTACGACAATTGGCTTTTATTGTGAAACATTGGGCCAAGTCGAGAGGAGTCAATGAAACTTACCATGGAACCCTATCTAGCTATGCGTATGTTTTGATGTGTATACATTTCTTACAACAGCGGAGACCTGCTATTCTTCCCTGTTTACAGGGAATGCGGACAACATACTCGATCACTGTAGAGAACATTGAATGTGCTTTCTTTGATCAAGTAGACAAACTTCAAGAATTTGGATCCCATAACAGGGAAACTATTGCTGAACTTGTGTGGGCTTTTTTCAATTACTGGGCATATAGTCATGACTACGCAAATACTGTTATATCCGTTCGTAGAGGAAGCACAATCAGCAAGCGGGAAAAGGACTGGACAAGGAGGATTGGAAATGACCGGCATTTGATATGCATAGAGGATCCATTTGAGACGTCCCATGACCTGGGACGAGTTGTAGACAAGCACAGCATAAAAGTTTTGAGGGAGGAGTTTGAACGTGCCGCTGAGATAATGCAGTATGATGCAAATCCCTGTGTGAAGCTCTTTGAACCCTATGTTCCTAGTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006971 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009504 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009861 GO:0009862 GO:0009863 GO:0009867 GO:0009868 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0014070 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031122 GO:0032260 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032870 GO:0033206 GO:0033993 GO:0035556 GO:0035690 GO:0035821 GO:0036211 GO:0042221 GO:0042493 GO:0042539 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0046677 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051321 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052306 GO:0052308 GO:0052552 GO:0052553 GO:0052564 GO:0052572 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071840 GO:0075136 GO:0080026 GO:0097305 GO:0097435 GO:0098542 GO:0140013 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1901701 GO:1902410 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04016 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04138 ko04139 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05206 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 ko05418 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04016 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04138 map04139 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05206 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

732

Amino Acids

81.78

Weight (kDa)

6.84

Isoelectric Point (pI)

60.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 413 - 541 1.8e-32 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
TUTase PF19088 421 - 523 2.6e-09 TUTase nucleotidyltransferase domain
PAP_assoc PF03828 631 - 689 1.2e-19 Cid1 family poly A polymerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2063
Acc36I ACCTGC 1 cut(s) 1774
AccB1I GGYRCC 1 cut(s) 968
AccB7I CCANNNNNTGG 2 cut(s) 965, 2071
AccI GTMKAC 3 cut(s) 1741, 1851, 2085
AccII CGCG 3 cut(s) 23, 636, 1510
AccIII TCCGGA 1 cut(s) 529
AclI AACGTT 1 cut(s) 173
AclWI GGATC 9 cut(s) 134, 211, 1369, 1535, 1548, 1866, 1879, 2042, 2055
AcoI YGGCCR 4 cut(s) 3, 150, 194, 223
AcsI RAATTY 8 cut(s) 56, 607, 640, 830, 900, 1018, 1245, 1865
AcuI CTGAAG 3 cut(s) 522, 582, 1359
AcyI GRCGYC 2 cut(s) 38, 2060
AdeI CACNNNGTG 1 cut(s) 802
AfaI GTAC 2 cut(s) 1187, 1497
AflIII ACRYGT 1 cut(s) 1508
AgsI TTSAA 9 cut(s) 607, 1004, 1244, 1253, 1829, 1862, 1918, 2123, 2177
AjnI CCWGG 5 cut(s) 99, 284, 508, 952, 2070
AluBI AGCT 8 cut(s) 490, 574, 943, 1343, 1534, 1606, 1719, 2170
AluI AGCT 8 cut(s) 490, 574, 943, 1343, 1534, 1606, 1719, 2170
Alw21I GWGCWC 2 cut(s) 798, 940
Alw26I GTCTC 2 cut(s) 1756, 2051
Alw44I GTGCAC 1 cut(s) 936
AlwI GGATC 9 cut(s) 134, 211, 1369, 1535, 1548, 1866, 1879, 2042, 2055
Aor13HI TCCGGA 1 cut(s) 529
AoxI GGCC 9 cut(s) 3, 81, 150, 160, 194, 223, 287, 1334, 1671
ApaI GGGCCC 1 cut(s) 164
ApaLI GTGCAC 1 cut(s) 936
ApeKI GCWGC 3 cut(s) 181, 384, 387
ApoI RAATTY 8 cut(s) 56, 607, 640, 830, 900, 1018, 1245, 1865
ArsI GACNNNNNNTTYG 2 cut(s) 1189, 1221
AspA2I CCTAGG 2 cut(s) 322, 746
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 8 cut(s) 82, 160, 161, 288, 589, 956, 1519, 1671
AsuC2I CCSGG 3 cut(s) 399, 729, 772
AsuHPI GGTGA 7 cut(s) 29, 145, 239, 899, 1073, 1426, 1433
AvaII GGWCC 3 cut(s) 589, 956, 1519
AvrII CCTAGG 2 cut(s) 322, 746
BaeGI GKGCMC 2 cut(s) 164, 940
BamHI GGATCC 3 cut(s) 1540, 1871, 2047
BanI GGYRCC 1 cut(s) 968
BanII GRGCYC 2 cut(s) 164, 933
BarI GAAGNNNNNNTAC 2 cut(s) 1842, 1874
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 2 cut(s) 798, 940
BbvI GCAGC 3 cut(s) 193, 396, 399
BccI CCATC 2 cut(s) 887, 1022
BceAI ACGGC 5 cut(s) 61, 131, 140, 181, 238
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 5 cut(s) 101, 286, 510, 954, 2072
BclI TGATCA 1 cut(s) 1843
BcnI CCSGG 3 cut(s) 399, 729, 772
BcoDI GTCTC 2 cut(s) 1756, 2051
BfaI CTAG 8 cut(s) 323, 747, 825, 918, 1487, 1515, 1716, 2190
BfmI CTRYAG 2 cut(s) 464, 1815
BfuAI ACCTGC 1 cut(s) 1774
BglII AGATCT 2 cut(s) 1402, 1446
BlnI CCTAGG 2 cut(s) 322, 746
Bme1390I CCNGG 8 cut(s) 101, 286, 399, 510, 729, 772, 954, 2072
Bme18I GGWCC 3 cut(s) 589, 956, 1519
BmgT120I GGNCC 8 cut(s) 82, 160, 161, 288, 589, 956, 1519, 1671
BmrFI CCNGG 8 cut(s) 101, 286, 399, 510, 729, 772, 954, 2072
BmrI ACTGGG 3 cut(s) 370, 890, 1933
BmsI GCATC 4 cut(s) 838, 1581, 1621, 2140
BmuI ACTGGG 3 cut(s) 370, 890, 1933
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 59
BpuEI CTTGAG 2 cut(s) 476, 1106
BpuMI CCSGG 3 cut(s) 399, 729, 772
Bsa29I ATCGAT 1 cut(s) 138
BsaBI GATNNNNATC 2 cut(s) 1401, 2154
BsaHI GRCGYC 2 cut(s) 38, 2060
BsaI GGTCTC 1 cut(s) 1756
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 4 cut(s) 329, 359, 772, 802
Bse118I RCCGGY 3 cut(s) 5, 34, 2025
Bse1I ACTGG 5 cut(s) 376, 885, 1555, 1928, 2006
Bse8I GATNNNNATC 2 cut(s) 1401, 2154
BseAI TCCGGA 1 cut(s) 529
BseBI CCWGG 5 cut(s) 101, 286, 510, 954, 2072
BseCI ATCGAT 1 cut(s) 138
BseGI GGATG 6 cut(s) 215, 232, 853, 1033, 1096, 1105
BseJI GATNNNNATC 2 cut(s) 1401, 2154
BseMII CTCAG 6 cut(s) 589, 615, 946, 1260, 1434, 2124
BseNI ACTGG 5 cut(s) 376, 885, 1555, 1928, 2006
BseSI GKGCMC 2 cut(s) 164, 940
BseX3I CGGCCG 2 cut(s) 194, 223
BseXI GCAGC 3 cut(s) 193, 396, 399
Bsh1236I CGCG 3 cut(s) 23, 636, 1510
Bsh1285I CGRYCG 2 cut(s) 197, 226
BshFI GGCC 9 cut(s) 5, 83, 152, 162, 196, 225, 289, 1336, 1673
BshNI GGYRCC 1 cut(s) 968
BshVI ATCGAT 1 cut(s) 138
BsiEI CGRYCG 2 cut(s) 197, 226
BsiHKAI GWGCWC 2 cut(s) 798, 940
BsiSI CCGG 7 cut(s) 6, 35, 399, 530, 728, 771, 2026
BslFI GGGAC 4 cut(s) 117, 1505, 2047, 2088
BsmAI GTCTC 2 cut(s) 1756, 2051
BsmBI CGTCTC 1 cut(s) 2051
BsmFI GGGAC 4 cut(s) 117, 1505, 2047, 2088
BsmI GAATGC 1 cut(s) 1797
BsnI GGCC 9 cut(s) 5, 83, 152, 162, 196, 225, 289, 1336, 1673
Bso31I GGTCTC 1 cut(s) 1756
Bsp120I GGGCCC 1 cut(s) 160
Bsp1286I GDGCHC 4 cut(s) 164, 798, 933, 940
Bsp13I TCCGGA 1 cut(s) 529
Bsp1407I TGTACA 1 cut(s) 1495
Bsp19I CCATGG 1 cut(s) 1702
BspANI GGCC 9 cut(s) 5, 83, 152, 162, 196, 225, 289, 1336, 1673
BspCNI CTCAG 6 cut(s) 588, 616, 945, 1261, 1435, 2125
BspDI ATCGAT 1 cut(s) 138
BspEI TCCGGA 1 cut(s) 529
BspFNI CGCG 3 cut(s) 23, 636, 1510
BspHI TCATGA 1 cut(s) 1936
BspMAI CTGCAG 1 cut(s) 468
BspMI ACCTGC 1 cut(s) 1774
BspPI GGATC 9 cut(s) 134, 211, 1369, 1535, 1548, 1866, 1879, 2042, 2055
BspQI GCTCTTC 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 968
BspTNI GGTCTC 1 cut(s) 1756
BsrFI RCCGGY 3 cut(s) 5, 34, 2025
BsrGI TGTACA 1 cut(s) 1495
BsrI ACTGG 5 cut(s) 376, 885, 1555, 1928, 2006
BssAI RCCGGY 3 cut(s) 5, 34, 2025
BssNAI GTATAC 1 cut(s) 1742
BssNI GRCGYC 2 cut(s) 38, 2060
BssT1I CCWWGG 3 cut(s) 322, 746, 1702
Bst1107I GTATAC 1 cut(s) 1742
Bst2UI CCWGG 5 cut(s) 101, 286, 510, 954, 2072
Bst4CI ACNGT 6 cut(s) 133, 169, 257, 1458, 1816, 1954
Bst6I CTCTTC 2 cut(s) 249, 999
BstACI GRCGYC 2 cut(s) 38, 2060
BstAUI TGTACA 1 cut(s) 1495
BstC8I GCNNGC 7 cut(s) 36, 110, 967, 1286, 1345, 1512, 1988
BstDEI CTNAG 7 cut(s) 575, 624, 932, 1166, 1269, 1443, 2133
BstDSI CCRYGG 4 cut(s) 147, 156, 197, 1702
BstENI CCTNNNNNAGG 1 cut(s) 750
BstF5I GGATG 6 cut(s) 215, 232, 853, 1033, 1096, 1105
BstFNI CGCG 3 cut(s) 23, 636, 1510
BstHHI GCGC 1 cut(s) 25
BstMAI GTCTC 2 cut(s) 1756, 2051
BstMCI CGRYCG 2 cut(s) 197, 226
BstMWI GCNNNNNNNGC 7 cut(s) 31, 152, 472, 591, 687, 690, 1983
BstNI CCWGG 5 cut(s) 101, 286, 510, 954, 2072
BstSCI CCNGG 8 cut(s) 99, 284, 397, 508, 727, 770, 952, 2070
BstSFI CTRYAG 2 cut(s) 464, 1815
BstSLI GKGCMC 2 cut(s) 164, 940
BstUI CGCG 3 cut(s) 23, 636, 1510
BstV1I GCAGC 3 cut(s) 193, 396, 399
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 5 cut(s) 1402, 1446, 1540, 1871, 2047
BstYI RGATCY 5 cut(s) 1402, 1446, 1540, 1871, 2047
BstZ17I GTATAC 1 cut(s) 1742
BstZI CGGCCG 2 cut(s) 194, 223
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 9 cut(s) 5, 83, 152, 162, 196, 225, 289, 1336, 1673
BsuTUI ATCGAT 1 cut(s) 138
BtgI CCRYGG 4 cut(s) 147, 156, 197, 1702
BtsCI GGATG 6 cut(s) 215, 232, 853, 1033, 1096, 1105
BtsIMutI CAGTG 3 cut(s) 940, 1548, 1812
BveI ACCTGC 1 cut(s) 1774
Cac8I GCNNGC 7 cut(s) 36, 110, 967, 1286, 1345, 1512, 1988
CciI TCATGA 1 cut(s) 1936
CfoI GCGC 1 cut(s) 25
Cfr10I RCCGGY 3 cut(s) 5, 34, 2025
Cfr13I GGNCC 8 cut(s) 82, 160, 161, 288, 589, 956, 1519, 1671
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 4 cut(s) 27, 29, 114, 1516
Csp6I GTAC 2 cut(s) 1186, 1496
CviAII CATG 4 cut(s) 1365, 1703, 1937, 2066
CviQI GTAC 2 cut(s) 1186, 1496
DdeI CTNAG 7 cut(s) 575, 624, 932, 1166, 1269, 1443, 2133
DraIII CACNNNGTG 1 cut(s) 802
EaeI YGGCCR 4 cut(s) 3, 150, 194, 223
EagI CGGCCG 2 cut(s) 194, 223
Eam1104I CTCTTC 2 cut(s) 249, 999
EarI CTCTTC 2 cut(s) 249, 999
EciI GGCGGA 1 cut(s) 27
EclXI CGGCCG 2 cut(s) 194, 223
Eco130I CCWWGG 3 cut(s) 322, 746, 1702
Eco24I GRGCYC 2 cut(s) 164, 933
Eco31I GGTCTC 1 cut(s) 1756
Eco47I GGWCC 3 cut(s) 589, 956, 1519
Eco52I CGGCCG 2 cut(s) 194, 223
Eco57I CTGAAG 3 cut(s) 522, 582, 1359
EcoNI CCTNNNNNAGG 1 cut(s) 750
EcoO109I RGGNCCY 3 cut(s) 82, 161, 1519
EcoRI GAATTC 3 cut(s) 56, 607, 900
EcoRII CCWGG 5 cut(s) 99, 284, 508, 952, 2070
EcoT14I CCWWGG 3 cut(s) 322, 746, 1702
EcoT22I ATGCAT 2 cut(s) 1574, 2042
EcoT38I GRGCYC 2 cut(s) 164, 933
ErhI CCWWGG 3 cut(s) 322, 746, 1702
Esp3I CGTCTC 1 cut(s) 2051
FaeI CATG 4 cut(s) 1368, 1706, 1940, 2069
FalI AAGNNNNNCTT 2 cut(s) 1332, 1364
FaqI GGGAC 4 cut(s) 117, 1505, 2047, 2088
FatI CATG 4 cut(s) 1364, 1702, 1936, 2065
FauI CCCGC 5 cut(s) 93, 151, 629, 970, 1983
FbaI TGATCA 1 cut(s) 1843
FblI GTMKAC 3 cut(s) 1741, 1851, 2085
FokI GGATG 6 cut(s) 202, 219, 860, 1040, 1103, 1112
FriOI GRGCYC 2 cut(s) 164, 933
FspBI CTAG 8 cut(s) 323, 747, 825, 918, 1487, 1515, 1716, 2190
GlaI GCGC 1 cut(s) 24
GsuI CTGGAG 1 cut(s) 59
HaeIII GGCC 9 cut(s) 5, 83, 152, 162, 196, 225, 289, 1336, 1673
HapII CCGG 7 cut(s) 6, 35, 399, 530, 728, 771, 2026
HgaI GACGC 4 cut(s) 27, 29, 114, 1516
HhaI GCGC 1 cut(s) 25
Hin1I GRCGYC 2 cut(s) 38, 2060
Hin1II CATG 4 cut(s) 1368, 1706, 1940, 2069
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HindIII AAGCTT 2 cut(s) 1532, 1604
HpaII CCGG 7 cut(s) 6, 35, 399, 530, 728, 771, 2026
HphI GGTGA 7 cut(s) 29, 145, 239, 899, 1073, 1426, 1433
Hpy166II GTNNAC 7 cut(s) 805, 938, 959, 1742, 1784, 1852, 2086
Hpy8I GTNNAC 7 cut(s) 805, 938, 959, 1742, 1784, 1852, 2086
Hpy99I CGWCG 4 cut(s) 211, 214, 422, 636
HpyAV CCTTC 3 cut(s) 73, 291, 328
HpyCH4III ACNGT 6 cut(s) 133, 169, 257, 1458, 1816, 1954
HpyCH4IV ACGT 3 cut(s) 173, 2060, 2125
HpyF10VI GCNNNNNNNGC 7 cut(s) 31, 152, 472, 591, 687, 690, 1983
HpyF3I CTNAG 7 cut(s) 575, 624, 932, 1166, 1269, 1443, 2133
HpySE526I ACGT 3 cut(s) 173, 2060, 2125
Hsp92I GRCGYC 2 cut(s) 38, 2060
Hsp92II CATG 4 cut(s) 1368, 1706, 1940, 2069
HspAI GCGC 1 cut(s) 23
KflI GGGWCCC 1 cut(s) 1519
Kpn2I TCCGGA 1 cut(s) 529
KroI GCCGGC 1 cut(s) 34
KroNI GCCGGC 1 cut(s) 36
Ksp22I TGATCA 1 cut(s) 1843
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 4 cut(s) 78, 655, 793, 1237
Lsp1109I GCAGC 3 cut(s) 193, 396, 399
LweI GCATC 4 cut(s) 838, 1581, 1621, 2140
MaeI CTAG 8 cut(s) 323, 747, 825, 918, 1487, 1515, 1716, 2190
MaeII ACGT 3 cut(s) 173, 2060, 2125
MaeIII GTNAC 3 cut(s) 17, 169, 1546
MfeI CAATTG 1 cut(s) 1646
MflI RGATCY 5 cut(s) 1402, 1446, 1540, 1871, 2047
MhlI GDGCHC 4 cut(s) 164, 798, 933, 940
MluI ACGCGT 1 cut(s) 1508
MlyI GAGTC 4 cut(s) 439, 542, 1016, 1695
MmeI TCCRAC 3 cut(s) 243, 636, 1290
Mph1103I ATGCAT 2 cut(s) 1574, 2042
MroI TCCGGA 1 cut(s) 529
MroNI GCCGGC 1 cut(s) 34
MseI TTAA 1 cut(s) 1434
MspA1I CMGCKG 3 cut(s) 965, 1759, 2132
MspI CCGG 7 cut(s) 6, 35, 399, 530, 728, 771, 2026
MspR9I CCNGG 8 cut(s) 101, 286, 399, 510, 729, 772, 954, 2072
MunI CAATTG 1 cut(s) 1646
Mva1269I GAATGC 1 cut(s) 1797
MvaI CCWGG 5 cut(s) 101, 286, 510, 954, 2072
MvnI CGCG 3 cut(s) 23, 636, 1510
MwoI GCNNNNNNNGC 7 cut(s) 31, 152, 472, 591, 687, 690, 1983
NaeI GCCGGC 1 cut(s) 36
NciI CCSGG 3 cut(s) 399, 729, 772
NcoI CCATGG 1 cut(s) 1702
NgoMIV GCCGGC 1 cut(s) 34
NlaIII CATG 4 cut(s) 1368, 1706, 1940, 2069
NmeAIII GCCGAG 1 cut(s) 251
NmuCI GTSAC 2 cut(s) 17, 1546
NsiI ATGCAT 2 cut(s) 1574, 2042
PagI TCATGA 1 cut(s) 1936
PciSI GCTCTTC 1 cut(s) 249
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 144, 449
PctI GAATGC 1 cut(s) 1797
PdiI GCCGGC 1 cut(s) 36
PfeI GAWTC 7 cut(s) 187, 271, 647, 662, 767, 1133, 1253
PflMI CCANNNNNTGG 2 cut(s) 965, 2071
PfoI TCCNGGA 1 cut(s) 770
PleI GAGTC 4 cut(s) 439, 541, 1015, 1694
PpsI GAGTC 4 cut(s) 439, 541, 1015, 1694
PpuMI RGGWCCY 1 cut(s) 1519
Psp1406I AACGTT 1 cut(s) 173
Psp5II RGGWCCY 1 cut(s) 1519
Psp6I CCWGG 5 cut(s) 99, 284, 508, 952, 2070
PspGI CCWGG 5 cut(s) 99, 284, 508, 952, 2070
PspOMI GGGCCC 1 cut(s) 160
PspPI GGNCC 8 cut(s) 82, 160, 161, 288, 589, 956, 1519, 1671
PspPPI RGGWCCY 1 cut(s) 1519
PstI CTGCAG 1 cut(s) 468
PsuI RGATCY 5 cut(s) 1402, 1446, 1540, 1871, 2047
RsaI GTAC 2 cut(s) 1187, 1497
RsaNI GTAC 2 cut(s) 1186, 1496
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 1434
Sau96I GGNCC 8 cut(s) 82, 160, 161, 288, 589, 956, 1519, 1671
SchI GAGTC 4 cut(s) 439, 542, 1016, 1695
ScrFI CCNGG 8 cut(s) 101, 286, 399, 510, 729, 772, 954, 2072
SduI GDGCHC 4 cut(s) 164, 798, 933, 940
SfaNI GCATC 4 cut(s) 838, 1581, 1621, 2140
SfcI CTRYAG 2 cut(s) 464, 1815
SinI GGWCC 3 cut(s) 589, 956, 1519
SmlI CTYRAG 2 cut(s) 491, 1085
SmoI CTYRAG 2 cut(s) 491, 1085
SspMI CTAG 8 cut(s) 323, 747, 825, 918, 1487, 1515, 1716, 2190
StyD4I CCNGG 8 cut(s) 99, 284, 397, 508, 727, 770, 952, 2070
StyI CCWWGG 3 cut(s) 322, 746, 1702
TaaI ACNGT 6 cut(s) 133, 169, 257, 1458, 1816, 1954
TaiI ACGT 3 cut(s) 176, 2063, 2128
TatI WGTACW 1 cut(s) 1495
TfiI GAWTC 7 cut(s) 187, 271, 647, 662, 767, 1133, 1253
Tru1I TTAA 1 cut(s) 1434
Tru9I TTAA 1 cut(s) 1434
TscAI CASTG 3 cut(s) 940, 1555, 1819
TseFI GTSAC 2 cut(s) 17, 1546
TseI GCWGC 3 cut(s) 181, 384, 387
Tsp45I GTSAC 2 cut(s) 17, 1546
TspDTI ATGAA 6 cut(s) 179, 609, 1031, 1107, 1365, 1707
TspGWI ACGGA 2 cut(s) 1112, 1951
TspRI CASTG 3 cut(s) 940, 1555, 1819
Van91I CCANNNNNTGG 2 cut(s) 965, 2071
VneI GTGCAC 1 cut(s) 936
VpaK11BI GGWCC 3 cut(s) 589, 956, 1519
XagI CCTNNNNNAGG 1 cut(s) 750
XapI RAATTY 8 cut(s) 56, 607, 640, 830, 900, 1018, 1245, 1865
XbaI TCTAGA 1 cut(s) 1486
XmaJI CCTAGG 2 cut(s) 322, 746
XmiI GTMKAC 3 cut(s) 1741, 1851, 2085
XspI CTAG 8 cut(s) 323, 747, 825, 918, 1487, 1515, 1716, 2190
ZraI GACGTC 1 cut(s) 2061
Zsp2I ATGCAT 2 cut(s) 1574, 2042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.