Rh5DG553600

Protein BPS1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0003642
Physical Location & Seq
Forward (+)
85479833 .. 85480877
1045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG553600.1

Sequence Viewer

Length: 720 bp
ATGAGTCGTCCACAGGTCCCGCACCGACCTTTCTTCCATTTTGGAAATCCTTTCCGGATGATTTCGCCGAAGGGTTCTCAAATGACTTCGAACCTTTCTGCACTGATGAACACTTTTGAGGAAACATTGGCTGGGAGGTTGAGAAAGCTCAACCCAAAAGATAACAATGATGTCCTTAGCTTATCATGGATGAAATTGGCAATGGAGTCGCTTTGTGGAACTCATAATGACATAAAAACTCTCATAGCTGAGATTGATCTTCCCGTGAGTGCCTGGGATGAGAAGTGGATTGATGTGTACTTGGACATCAGTGTAAAATTGCTCGATATATGCATTGTCTTCAGCTCTGAGATGGCACGCTTAAACCAGGGGCATTTGTTTCTTCAGTTTGTGTTGCATAATTTGAATTCAGCCTCTTCGAAGCAATTTATTCATGCCCGTTCTTTAGTTGATGACTGGAAGAATCATATTGCTTCAAAGAACCCCAGGGTTGAGAACTGTAGCACCATTTTGGACAAGCTTGTGGAATCACTGGATCTACCAAAGGTTAAGAACTCAGCAAAAGGGAAACTTTTAATGCGTGCTATGTATGGAGTGAAGGTGTTGACAGTATCTGTTTGTAGTGTCTTTGCCGCAGCCTTTTCTGGTTCTGCAAGCAAGTTGTTAGATTTGAATGTTGCCGAGACATATATGTGGGCACAAGCATTTAATGACTTATAG

Protein Analysis

239

Amino Acids

26.87

Weight (kDa)

8.64

Isoelectric Point (pI)

32.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 36 - 137 6.1e-06 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 54
AciI CCGC 2 cut(s) 20, 633
AclWI GGATC 1 cut(s) 543
AcsI RAATTY 1 cut(s) 406
AcuI CTGAAG 2 cut(s) 325, 368
AfaI GTAC 1 cut(s) 299
AgsI TTSAA 3 cut(s) 406, 477, 673
AjnI CCWGG 3 cut(s) 272, 366, 485
AluBI AGCT 5 cut(s) 148, 180, 248, 345, 520
AluI AGCT 5 cut(s) 148, 180, 248, 345, 520
Alw26I GTCTC 1 cut(s) 677
AlwI GGATC 1 cut(s) 543
AlwNI CAGNNNCTG 1 cut(s) 614
Aor13HI TCCGGA 1 cut(s) 54
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 406
AspS9I GGNCC 1 cut(s) 16
AsuII TTCGAA 2 cut(s) 89, 419
AvaII GGWCC 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 700
BbsI GAAGAC 1 cut(s) 331
BbvI GCAGC 1 cut(s) 647
BccI CCATC 1 cut(s) 346
BcgI CGANNNNNNTGC 2 cut(s) 189, 223
BciT130I CCWGG 3 cut(s) 274, 368, 487
BcoDI GTCTC 1 cut(s) 677
BfmI CTRYAG 1 cut(s) 499
BisI GCNGC 2 cut(s) 633, 636
BlsI GCNGC 2 cut(s) 634, 637
Bme1390I CCNGG 3 cut(s) 274, 368, 487
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 3 cut(s) 274, 368, 487
BpiI GAAGAC 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 176
Bpu14I TTCGAA 2 cut(s) 89, 419
BsaJI CCNNGG 4 cut(s) 273, 367, 485, 486
BsaWI WCCGGW 1 cut(s) 54
BsaXI ACNNNNNCTCC 2 cut(s) 585, 615
Bse1I ACTGG 2 cut(s) 461, 537
Bse3DI GCAATG 1 cut(s) 207
BseAI TCCGGA 1 cut(s) 54
BseBI CCWGG 3 cut(s) 274, 368, 487
BseDI CCNNGG 4 cut(s) 273, 367, 485, 486
BseGI GGATG 3 cut(s) 63, 195, 283
BseMI GCAATG 1 cut(s) 207
BseMII CTCAG 3 cut(s) 240, 339, 570
BseNI ACTGG 2 cut(s) 461, 537
BseSI GKGCMC 1 cut(s) 700
BseXI GCAGC 1 cut(s) 647
BseYI CCCAGC 1 cut(s) 131
BsgI GTGCAG 1 cut(s) 84
BsiSI CCGG 1 cut(s) 55
BslFI GGGAC 1 cut(s) 2
BsmAI GTCTC 1 cut(s) 677
BsmFI GGGAC 1 cut(s) 2
Bsp119I TTCGAA 2 cut(s) 89, 419
Bsp1286I GDGCHC 1 cut(s) 700
Bsp13I TCCGGA 1 cut(s) 54
Bsp143I GATC 2 cut(s) 256, 535
BspACI CCGC 2 cut(s) 20, 633
BspCNI CTCAG 3 cut(s) 241, 340, 569
BspEI TCCGGA 1 cut(s) 54
BspLI GGNNCC 1 cut(s) 18
BspPI GGATC 1 cut(s) 543
BspT104I TTCGAA 2 cut(s) 89, 419
BsrDI GCAATG 1 cut(s) 207
BsrI ACTGG 2 cut(s) 461, 537
BssECI CCNNGG 4 cut(s) 273, 367, 485, 486
BssMI GATC 2 cut(s) 256, 535
Bst2UI CCWGG 3 cut(s) 274, 368, 487
Bst4CI ACNGT 2 cut(s) 500, 610
Bst6I CTCTTC 1 cut(s) 421
BstBI TTCGAA 2 cut(s) 89, 419
BstC8I GCNNGC 3 cut(s) 358, 582, 655
BstDEI CTNAG 4 cut(s) 176, 249, 348, 556
BstF5I GGATG 3 cut(s) 63, 195, 283
BstKTI GATC 2 cut(s) 259, 538
BstMAI GTCTC 1 cut(s) 677
BstMBI GATC 2 cut(s) 256, 535
BstNI CCWGG 3 cut(s) 274, 368, 487
BstSCI CCNGG 3 cut(s) 272, 366, 485
BstSFI CTRYAG 1 cut(s) 499
BstSLI GKGCMC 1 cut(s) 700
BstV1I GCAGC 1 cut(s) 647
BstV2I GAAGAC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 535
BstYI RGATCY 1 cut(s) 535
BtsCI GGATG 3 cut(s) 63, 195, 283
BtsIMutI CAGTG 3 cut(s) 101, 316, 530
Cac8I GCNNGC 3 cut(s) 358, 582, 655
CaiI CAGNNNCTG 1 cut(s) 614
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 1 cut(s) 298
CviAII CATG 2 cut(s) 186, 434
CviJI RGCY 8 cut(s) 131, 148, 180, 248, 345, 413, 520, 638
CviKI_1 RGCY 8 cut(s) 131, 148, 180, 248, 345, 413, 520, 638
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 4 cut(s) 176, 249, 348, 556
DpnI GATC 2 cut(s) 258, 537
DpnII GATC 2 cut(s) 256, 535
Eam1104I CTCTTC 1 cut(s) 421
EarI CTCTTC 1 cut(s) 421
Eco47I GGWCC 1 cut(s) 16
Eco57I CTGAAG 2 cut(s) 325, 368
EcoO109I RGGNCCY 1 cut(s) 16
EcoRI GAATTC 1 cut(s) 406
EcoRII CCWGG 3 cut(s) 272, 366, 485
EcoT22I ATGCAT 1 cut(s) 335
FaeI CATG 2 cut(s) 189, 437
FalI AAGNNNNNCTT 2 cut(s) 555, 587
FaqI GGGAC 1 cut(s) 2
FatI CATG 2 cut(s) 185, 433
FauI CCCGC 1 cut(s) 27
Fnu4HI GCNGC 2 cut(s) 633, 636
FokI GGATG 3 cut(s) 70, 202, 290
Fsp4HI GCNGC 2 cut(s) 633, 636
GluI GCNGC 2 cut(s) 633, 636
GsaI CCCAGC 1 cut(s) 135
HapII CCGG 1 cut(s) 55
Hin1II CATG 2 cut(s) 189, 437
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 4 cut(s) 4, 206, 463, 527
HpaII CCGG 1 cut(s) 55
Hpy166II GTNNAC 3 cut(s) 11, 298, 606
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 1 cut(s) 55
Hpy8I GTNNAC 3 cut(s) 11, 298, 606
HpyAV CCTTC 2 cut(s) 64, 592
HpyCH4III ACNGT 2 cut(s) 500, 610
HpyCH4V TGCA 4 cut(s) 101, 333, 397, 653
HpyF3I CTNAG 4 cut(s) 176, 249, 348, 556
Hsp92II CATG 2 cut(s) 189, 437
Kpn2I TCCGGA 1 cut(s) 54
Kzo9I GATC 2 cut(s) 256, 535
Lsp1109I GCAGC 1 cut(s) 647
MalI GATC 2 cut(s) 258, 537
MboI GATC 2 cut(s) 256, 535
MboII GAAGA 6 cut(s) 25, 251, 331, 374, 408, 472
MflI RGATCY 1 cut(s) 535
MhlI GDGCHC 1 cut(s) 700
MluCI AATT 5 cut(s) 194, 317, 400, 406, 425
MlyI GAGTC 2 cut(s) 13, 215
MnlI CCTC 3 cut(s) 112, 129, 424
Mph1103I ATGCAT 1 cut(s) 335
MroI TCCGGA 1 cut(s) 54
MseI TTAA 4 cut(s) 362, 549, 575, 708
MslI CAYNNNNRTG 1 cut(s) 691
MspI CCGG 1 cut(s) 55
MspR9I CCNGG 3 cut(s) 274, 368, 487
MvaI CCWGG 3 cut(s) 274, 368, 487
NdeII GATC 2 cut(s) 256, 535
NlaIII CATG 2 cut(s) 189, 437
NlaIV GGNNCC 1 cut(s) 18
NmeAIII GCCGAG 1 cut(s) 706
NsiI ATGCAT 1 cut(s) 335
NspV TTCGAA 2 cut(s) 89, 419
PasI CCCWGGG 1 cut(s) 486
PfeI GAWTC 2 cut(s) 463, 527
PkrI GCNGC 2 cut(s) 634, 637
PleI GAGTC 2 cut(s) 12, 214
PpsI GAGTC 2 cut(s) 12, 214
PpuMI RGGWCCY 1 cut(s) 16
Psp5II RGGWCCY 1 cut(s) 16
Psp6I CCWGG 3 cut(s) 272, 366, 485
PspFI CCCAGC 1 cut(s) 131
PspGI CCWGG 3 cut(s) 272, 366, 485
PspN4I GGNNCC 1 cut(s) 18
PspPI GGNCC 1 cut(s) 16
PspPPI RGGWCCY 1 cut(s) 16
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 1 cut(s) 535
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
RseI CAYNNNNRTG 1 cut(s) 691
SaqAI TTAA 4 cut(s) 362, 549, 575, 708
SatI GCNGC 2 cut(s) 633, 636
Sau3AI GATC 2 cut(s) 256, 535
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 2 cut(s) 13, 215
ScrFI CCNGG 3 cut(s) 274, 368, 487
SduI GDGCHC 1 cut(s) 700
SfcI CTRYAG 1 cut(s) 499
SfuI TTCGAA 2 cut(s) 89, 419
SinI GGWCC 1 cut(s) 16
SmiMI CAYNNNNRTG 1 cut(s) 691
Sse9I AATT 5 cut(s) 194, 317, 400, 406, 425
SsiI CCGC 2 cut(s) 20, 633
StyD4I CCNGG 3 cut(s) 272, 366, 485
TaaI ACNGT 2 cut(s) 500, 610
TaqI TCGA 3 cut(s) 89, 324, 419
TasI AATT 5 cut(s) 194, 317, 400, 406, 425
TatI WGTACW 1 cut(s) 297
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 2 cut(s) 463, 527
Tru1I TTAA 4 cut(s) 362, 549, 575, 708
Tru9I TTAA 4 cut(s) 362, 549, 575, 708
TscAI CASTG 3 cut(s) 108, 316, 537
TseI GCWGC 1 cut(s) 635
TspDTI ATGAA 3 cut(s) 122, 206, 422
TspRI CASTG 3 cut(s) 108, 316, 537
VpaK11BI GGWCC 1 cut(s) 16
XapI RAATTY 1 cut(s) 406
Zsp2I ATGCAT 1 cut(s) 335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.