Rh5AG212900

Protein BPS1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0003642
Physical Location & Seq
Forward (+)
25154435 .. 25155076
642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG212900.1

Sequence Viewer

Length: 642 bp
ATGATATTGGATTTTATGAAAAGCAAAGGATGCAACCCAAATGTATTCAATTACTCAGCTCTGATGAATGGATTCTGTAAGGAGGAACGTAATGACATAAAAACTCTCATAGCTGAGATTGATCTTCCCGTGAGTGCTTGGGATGAGAAGTGGATTGATGTGTACTTGGACATCAGTGTAAAGTTGCTCGATATATGCATTGTCTTCAGCTCTGAGATCGCACGCTTAAACCAGGGGCATTTGTTTCTTCAGTTTGTGTTGCATAATTTGAATTCAGCCTCTTCGAAGCAATTTATTCGTGCCCGTTCTTCAATTGATGACTGGAAGAATCATATTGCTTCAAAGAACCCCAGGGTTGAGAACTGTAGCACCATTTTGGACAAGCTTGTGGAATCACTGGATCTACCAAAGGTTAAGAACTCAGCAAAAGGGAAACTTTTAATGTGTGCTATGTATGGAGTGAAGGTGTTGACAGTATCTGTTTGTAGTGTCTTTACCGCAGCCTTTTCTGGTTCTGCAAGCAAGTTGTTAGATTTGAATGTTGCCGAGACATATATGTGGGCACAAGCATTTAATGACTTACAGAGTAATGTAAATGGGGAATTAGAAATGTACTTTCTAGTGGTAGAGTTACGGTACTGA

Protein Analysis

213

Amino Acids

24.07

Weight (kDa)

5.94

Isoelectric Point (pI)

28.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 9 - 32 3.2e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 498
AclWI GGATC 1 cut(s) 408
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 2 cut(s) 190, 233
AfaI GTAC 3 cut(s) 164, 614, 638
AgsI TTSAA 5 cut(s) 49, 271, 312, 342, 538
AjnI CCWGG 2 cut(s) 231, 350
AluBI AGCT 4 cut(s) 59, 113, 210, 385
AluI AGCT 4 cut(s) 59, 113, 210, 385
Alw26I GTCTC 1 cut(s) 542
AlwI GGATC 1 cut(s) 408
AlwNI CAGNNNCTG 1 cut(s) 479
ApeKI GCWGC 1 cut(s) 500
ApoI RAATTY 1 cut(s) 271
Asp700I GAANNNNTTC 1 cut(s) 71
AsuII TTCGAA 1 cut(s) 284
BaeGI GKGCMC 2 cut(s) 304, 565
BbsI GAAGAC 1 cut(s) 196
BbvI GCAGC 1 cut(s) 512
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 2 cut(s) 233, 352
BcoDI GTCTC 1 cut(s) 542
BfaI CTAG 1 cut(s) 620
BfmI CTRYAG 1 cut(s) 364
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 2 cut(s) 233, 352
BmrFI CCNGG 2 cut(s) 233, 352
BmsI GCATC 1 cut(s) 20
BpiI GAAGAC 1 cut(s) 196
Bpu14I TTCGAA 1 cut(s) 284
BsaJI CCNNGG 3 cut(s) 232, 350, 351
BsaXI ACNNNNNCTCC 2 cut(s) 450, 480
Bse1I ACTGG 2 cut(s) 326, 402
BseBI CCWGG 2 cut(s) 233, 352
BseDI CCNNGG 3 cut(s) 232, 350, 351
BseGI GGATG 2 cut(s) 35, 148
BseMII CTCAG 4 cut(s) 69, 105, 204, 435
BseNI ACTGG 2 cut(s) 326, 402
BseSI GKGCMC 2 cut(s) 304, 565
BseXI GCAGC 1 cut(s) 512
BsmAI GTCTC 1 cut(s) 542
Bsp119I TTCGAA 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 304, 565
Bsp143I GATC 3 cut(s) 121, 216, 400
BspACI CCGC 1 cut(s) 498
BspCNI CTCAG 4 cut(s) 68, 106, 205, 434
BspPI GGATC 1 cut(s) 408
BspT104I TTCGAA 1 cut(s) 284
BsrI ACTGG 2 cut(s) 326, 402
BssECI CCNNGG 3 cut(s) 232, 350, 351
BssMI GATC 3 cut(s) 121, 216, 400
Bst2UI CCWGG 2 cut(s) 233, 352
Bst4CI ACNGT 3 cut(s) 365, 475, 636
Bst6I CTCTTC 1 cut(s) 286
BstAPI GCANNNNNTGC 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 284
BstC8I GCNNGC 2 cut(s) 223, 520
BstDEI CTNAG 4 cut(s) 55, 114, 213, 421
BstF5I GGATG 2 cut(s) 35, 148
BstKTI GATC 3 cut(s) 124, 219, 403
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 3 cut(s) 121, 216, 400
BstMWI GCNNNNNNNGC 1 cut(s) 30
BstNI CCWGG 2 cut(s) 233, 352
BstSCI CCNGG 2 cut(s) 231, 350
BstSFI CTRYAG 1 cut(s) 364
BstSLI GKGCMC 2 cut(s) 304, 565
BstV1I GCAGC 1 cut(s) 512
BstV2I GAAGAC 1 cut(s) 196
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
BtsCI GGATG 2 cut(s) 35, 148
BtsIMutI CAGTG 2 cut(s) 181, 395
Cac8I GCNNGC 2 cut(s) 223, 520
CaiI CAGNNNCTG 1 cut(s) 479
Csp6I GTAC 3 cut(s) 163, 613, 637
CviJI RGCY 6 cut(s) 59, 113, 210, 278, 385, 503
CviKI_1 RGCY 6 cut(s) 59, 113, 210, 278, 385, 503
CviQI GTAC 3 cut(s) 163, 613, 637
DdeI CTNAG 4 cut(s) 55, 114, 213, 421
DpnI GATC 3 cut(s) 123, 218, 402
DpnII GATC 3 cut(s) 121, 216, 400
Eam1104I CTCTTC 1 cut(s) 286
EarI CTCTTC 1 cut(s) 286
Eco57I CTGAAG 2 cut(s) 190, 233
EcoRI GAATTC 1 cut(s) 271
EcoRII CCWGG 2 cut(s) 231, 350
EcoT22I ATGCAT 1 cut(s) 200
FalI AAGNNNNNCTT 2 cut(s) 420, 452
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 2 cut(s) 42, 155
Fsp4HI GCNGC 1 cut(s) 501
FspBI CTAG 1 cut(s) 620
GluI GCNGC 1 cut(s) 501
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HindIII AAGCTT 1 cut(s) 383
HinfI GANTC 3 cut(s) 72, 328, 392
Hpy166II GTNNAC 2 cut(s) 163, 471
Hpy188I TCNGA 2 cut(s) 63, 214
Hpy8I GTNNAC 2 cut(s) 163, 471
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 3 cut(s) 365, 475, 636
HpyCH4IV ACGT 1 cut(s) 88
HpyCH4V TGCA 4 cut(s) 33, 198, 262, 518
HpyF10VI GCNNNNNNNGC 1 cut(s) 30
HpyF3I CTNAG 4 cut(s) 55, 114, 213, 421
HpySE526I ACGT 1 cut(s) 88
Kzo9I GATC 3 cut(s) 121, 216, 400
LpnPI CCDG 7 cut(s) 218, 245, 307, 337, 364, 383, 495
Lsp1109I GCAGC 1 cut(s) 512
LweI GCATC 1 cut(s) 20
MaeI CTAG 1 cut(s) 620
MaeII ACGT 1 cut(s) 88
MaeIII GTNAC 1 cut(s) 630
MalI GATC 3 cut(s) 123, 218, 402
MboI GATC 3 cut(s) 121, 216, 400
MboII GAAGA 6 cut(s) 116, 196, 239, 273, 300, 337
MfeI CAATTG 1 cut(s) 312
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 2 cut(s) 304, 565
MluCI AATT 6 cut(s) 49, 265, 271, 290, 312, 602
MnlI CCTC 2 cut(s) 76, 289
Mph1103I ATGCAT 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 4 cut(s) 227, 414, 440, 573
MslI CAYNNNNRTG 1 cut(s) 556
MspR9I CCNGG 2 cut(s) 233, 352
MunI CAATTG 1 cut(s) 312
MvaI CCWGG 2 cut(s) 233, 352
MwoI GCNNNNNNNGC 1 cut(s) 30
NdeII GATC 3 cut(s) 121, 216, 400
NmeAIII GCCGAG 1 cut(s) 571
NsiI ATGCAT 1 cut(s) 200
NspV TTCGAA 1 cut(s) 284
PasI CCCWGGG 1 cut(s) 351
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 3 cut(s) 72, 328, 392
PkrI GCNGC 1 cut(s) 502
Psp6I CCWGG 2 cut(s) 231, 350
PspGI CCWGG 2 cut(s) 231, 350
PstNI CAGNNNCTG 1 cut(s) 479
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 3 cut(s) 164, 614, 638
RsaNI GTAC 3 cut(s) 163, 613, 637
RseI CAYNNNNRTG 1 cut(s) 556
SaqAI TTAA 4 cut(s) 227, 414, 440, 573
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 3 cut(s) 121, 216, 400
ScrFI CCNGG 2 cut(s) 233, 352
SduI GDGCHC 2 cut(s) 304, 565
SetI ASST 7 cut(s) 61, 91, 115, 212, 387, 414, 468
SfaNI GCATC 1 cut(s) 20
SfcI CTRYAG 1 cut(s) 364
SfuI TTCGAA 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 556
Sse9I AATT 6 cut(s) 49, 265, 271, 290, 312, 602
SsiI CCGC 1 cut(s) 498
SspMI CTAG 1 cut(s) 620
StyD4I CCNGG 2 cut(s) 231, 350
TaaI ACNGT 3 cut(s) 365, 475, 636
TaiI ACGT 1 cut(s) 91
TaqI TCGA 2 cut(s) 189, 284
TasI AATT 6 cut(s) 49, 265, 271, 290, 312, 602
TatI WGTACW 2 cut(s) 162, 612
TfiI GAWTC 3 cut(s) 72, 328, 392
Tru1I TTAA 4 cut(s) 227, 414, 440, 573
Tru9I TTAA 4 cut(s) 227, 414, 440, 573
TscAI CASTG 2 cut(s) 181, 402
TseI GCWGC 1 cut(s) 500
TspDTI ATGAA 2 cut(s) 32, 80
TspRI CASTG 2 cut(s) 181, 402
XapI RAATTY 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 1 cut(s) 620
Zsp2I ATGCAT 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.