MD01G1069200.v1.1

Protein BPS1, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0003642
Physical Location & Seq
Reverse (-)
17323320 .. 17325883
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1069200.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGATTGCACCAAAGGGTTCCCAACTGTCTCCAAGGCTTGTTGGACTGTTGAACACGTTTGAGGAAACATTGGCTGGGAGGCTAAGAAAGCTTAACCCAAAAGACAAGGATGATGTCCTCAGCTTGTCATGGATGAAATTAGCCATGGAGTCTCTTTGTGGAACTCATAATGACATAAAATCCCTCATAGCTGAAATTGATCTCCCTGTGAGTAACTGGGACGAGAAATGGATTGATGTGTACTTGGACATCAGTGTGAAGTTGCTTGATGTATGCATTGCTTTTAGCTCTGAGATCTCACGTTTAAACCAGGGACATCTTTATCTTCAGTGTGTCTTGCATAATTTGGATTCAACTACTTCAGACCAATTTATTCGGGCCCGTTCTTCACTTGATGGCTGGAGGCATCATATTGGTTCAAAAAACCCTAGAGTTGAGAACTGTAGCACCATTTTAGATAAGCTTGTGGAATCCCTTGATCTGCCAAAGGTTAAGAACTCAGCCAAAGGGAAACTTTTGATGCGTGCTATGTATGGAGTGAAGGTGTTGACAATATCTGTTTGTAGTGTCTTTGCTGCCGCCTTTTCTGGTTCTGCAAAGAAGTTGTTAGATTTGAATGTTGCTGATACATATTTGTGGGCACAAGCGTTTAACGATTTACAGGGTAATGTAAATGGGGAAATTAGAAATGTACTTTCTAGTGGAAGAGTCATGGTACTGAAGGAGCTGGAAGCAGTTGATGATACTGTCAAGGAAATGGATCCCAAGATCCAGGATGGCGTTGACCTCAGTGAAAGGAATGCATTCAAGAATTCTATTTCAGACTTAGACAGGAAGGCACAGAAACTCTCCCAAGGGCTCGATCTTCTTACAAAGGAAGTTGATGGATTTTTCCAAATCCTTTTAGCCGGGCGTGACACATTGCTTTCCAAACTAAGATCAGGTGGAGCAGTCTCAGACCGGATGCTGACGGGAAATGTGGAAGGTCAGTTTGTGAGATGA

Protein Analysis

334

Amino Acids

36.92

Weight (kDa)

6.54

Isoelectric Point (pI)

22.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 17 - 128 1.5e-07 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 579
AclWI GGATC 3 cut(s) 755, 763, 768
AcsI RAATTY 1 cut(s) 811
AcuI CTGAAG 3 cut(s) 311, 345, 740
AfaI GTAC 3 cut(s) 242, 693, 717
AflIII ACRYGT 1 cut(s) 54
AgsI TTSAA 5 cut(s) 52, 354, 420, 616, 808
AjnI CCWGG 2 cut(s) 309, 771
AluBI AGCT 6 cut(s) 91, 123, 191, 288, 463, 727
AluI AGCT 6 cut(s) 91, 123, 191, 288, 463, 727
Alw26I GTCTC 3 cut(s) 33, 156, 958
AlwI GGATC 3 cut(s) 755, 763, 768
AoxI GGCC 1 cut(s) 378
ApaI GGGCCC 1 cut(s) 382
ApeKI GCWGC 1 cut(s) 575
ApoI RAATTY 1 cut(s) 811
Asp700I GAANNNNTTC 1 cut(s) 803
AspS9I GGNCC 2 cut(s) 378, 379
AsuC2I CCSGG 1 cut(s) 910
BaeGI GKGCMC 2 cut(s) 382, 643
BamHI GGATCC 1 cut(s) 760
BanII GRGCYC 2 cut(s) 382, 861
BbvCI CCTCAGC 1 cut(s) 119
BbvI GCAGC 1 cut(s) 562
BccI CCATC 3 cut(s) 389, 770, 878
BciT130I CCWGG 2 cut(s) 311, 773
BcnI CCSGG 1 cut(s) 910
BcoDI GTCTC 3 cut(s) 33, 156, 958
BfaI CTAG 2 cut(s) 429, 699
BfmI CTRYAG 1 cut(s) 442
BglII AGATCT 1 cut(s) 294
BisI GCNGC 2 cut(s) 576, 579
BlsI GCNGC 2 cut(s) 577, 580
Bme1390I CCNGG 3 cut(s) 311, 773, 910
BmgT120I GGNCC 2 cut(s) 378, 379
BmiI GGNNCC 3 cut(s) 19, 380, 762
BmrFI CCNGG 3 cut(s) 311, 773, 910
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 3 cut(s) 415, 510, 954
BmuI ACTGGG 1 cut(s) 226
BpmI CTGGAG 1 cut(s) 421
Bpu10I CCTNAGC 1 cut(s) 119
BpuMI CCSGG 1 cut(s) 910
BsaJI CCNNGG 4 cut(s) 32, 144, 310, 853
BsaWI WCCGGW 1 cut(s) 960
BsaXI ACNNNNNCTCC 2 cut(s) 528, 558
Bse1I ACTGG 1 cut(s) 221
Bse3DI GCAATG 2 cut(s) 276, 920
BseBI CCWGG 2 cut(s) 311, 773
BseDI CCNNGG 4 cut(s) 32, 144, 310, 853
BseGI GGATG 4 cut(s) 115, 138, 781, 969
BseMI GCAATG 2 cut(s) 276, 920
BseMII CTCAG 5 cut(s) 133, 282, 513, 802, 969
BseNI ACTGG 1 cut(s) 221
BseSI GKGCMC 2 cut(s) 382, 643
BseXI GCAGC 1 cut(s) 562
BseYI CCCAGC 1 cut(s) 74
BshFI GGCC 1 cut(s) 380
BsiSI CCGG 2 cut(s) 909, 961
BslFI GGGAC 2 cut(s) 233, 327
BsmAI GTCTC 3 cut(s) 33, 156, 958
BsmFI GGGAC 2 cut(s) 233, 327
BsmI GAATGC 2 cut(s) 803, 805
BsnI GGCC 1 cut(s) 380
Bsp120I GGGCCC 1 cut(s) 378
Bsp1286I GDGCHC 3 cut(s) 382, 643, 861
Bsp143I GATC 7 cut(s) 199, 294, 478, 760, 768, 862, 938
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 1 cut(s) 579
BspANI GGCC 1 cut(s) 380
BspCNI CTCAG 5 cut(s) 132, 283, 512, 801, 968
BspLI GGNNCC 3 cut(s) 19, 380, 762
BspPI GGATC 3 cut(s) 755, 763, 768
BsrDI GCAATG 2 cut(s) 276, 920
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 4 cut(s) 32, 144, 310, 853
BssMI GATC 7 cut(s) 199, 294, 478, 760, 768, 862, 938
BssT1I CCWWGG 3 cut(s) 32, 144, 853
Bst2UI CCWGG 2 cut(s) 311, 773
Bst4CI ACNGT 4 cut(s) 27, 48, 443, 748
Bst6I CTCTTC 1 cut(s) 700
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 8 cut(s) 83, 119, 291, 499, 788, 826, 935, 955
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 4 cut(s) 115, 138, 781, 969
BstKTI GATC 7 cut(s) 202, 297, 481, 763, 771, 865, 941
BstMAI GTCTC 3 cut(s) 33, 156, 958
BstMBI GATC 7 cut(s) 199, 294, 478, 760, 768, 862, 938
BstMWI GCNNNNNNNGC 1 cut(s) 88
BstNI CCWGG 2 cut(s) 311, 773
BstSCI CCNGG 3 cut(s) 309, 771, 908
BstSFI CTRYAG 1 cut(s) 442
BstSLI GKGCMC 2 cut(s) 382, 643
BstV1I GCAGC 1 cut(s) 562
BstX2I RGATCY 3 cut(s) 294, 760, 768
BstYI RGATCY 3 cut(s) 294, 760, 768
BsuRI GGCC 1 cut(s) 380
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 4 cut(s) 115, 138, 781, 969
BtsIMutI CAGTG 3 cut(s) 259, 335, 796
Cac8I GCNNGC 1 cut(s) 525
Cfr13I GGNCC 2 cut(s) 378, 379
Csp6I GTAC 3 cut(s) 241, 692, 716
CviAII CATG 3 cut(s) 129, 145, 712
CviQI GTAC 3 cut(s) 241, 692, 716
DdeI CTNAG 8 cut(s) 83, 119, 291, 499, 788, 826, 935, 955
DpnI GATC 7 cut(s) 201, 296, 480, 762, 770, 864, 940
DpnII GATC 7 cut(s) 199, 294, 478, 760, 768, 862, 938
DraI TTTAAA 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 700
EarI CTCTTC 1 cut(s) 700
Eco130I CCWWGG 3 cut(s) 32, 144, 853
Eco24I GRGCYC 2 cut(s) 382, 861
Eco57I CTGAAG 3 cut(s) 311, 345, 740
EcoRI GAATTC 1 cut(s) 811
EcoRII CCWGG 2 cut(s) 309, 771
EcoT14I CCWWGG 3 cut(s) 32, 144, 853
EcoT22I ATGCAT 2 cut(s) 278, 805
EcoT38I GRGCYC 2 cut(s) 382, 861
ErhI CCWWGG 3 cut(s) 32, 144, 853
FaeI CATG 3 cut(s) 132, 148, 715
FalI AAGNNNNNCTT 2 cut(s) 498, 530
FaqI GGGAC 2 cut(s) 233, 327
FatI CATG 3 cut(s) 128, 144, 711
Fnu4HI GCNGC 2 cut(s) 576, 579
FokI GGATG 4 cut(s) 122, 145, 788, 976
FriOI GRGCYC 2 cut(s) 382, 861
Fsp4HI GCNGC 2 cut(s) 576, 579
FspBI CTAG 2 cut(s) 429, 699
GluI GCNGC 2 cut(s) 576, 579
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 1 cut(s) 421
HaeIII GGCC 1 cut(s) 380
HapII CCGG 2 cut(s) 909, 961
Hin1II CATG 3 cut(s) 132, 148, 715
HincII GTYRAC 2 cut(s) 549, 784
HindII GTYRAC 2 cut(s) 549, 784
HindIII AAGCTT 2 cut(s) 89, 461
HinfI GANTC 4 cut(s) 149, 350, 470, 708
HpaII CCGG 2 cut(s) 909, 961
Hpy166II GTNNAC 3 cut(s) 241, 549, 784
Hpy188I TCNGA 4 cut(s) 292, 364, 823, 958
Hpy188III TCNNGA 1 cut(s) 808
Hpy8I GTNNAC 3 cut(s) 241, 549, 784
HpyAV CCTTC 4 cut(s) 535, 715, 829, 977
HpyCH4III ACNGT 4 cut(s) 27, 48, 443, 748
HpyCH4IV ACGT 2 cut(s) 56, 301
HpyCH4V TGCA 5 cut(s) 8, 276, 340, 596, 803
HpyF10VI GCNNNNNNNGC 1 cut(s) 88
HpyF3I CTNAG 8 cut(s) 83, 119, 291, 499, 788, 826, 935, 955
HpySE526I ACGT 2 cut(s) 56, 301
Hsp92II CATG 3 cut(s) 132, 148, 715
Kzo9I GATC 7 cut(s) 199, 294, 478, 760, 768, 862, 938
LmnI GCTCC 2 cut(s) 724, 947
Lsp1109I GCAGC 1 cut(s) 562
LweI GCATC 3 cut(s) 415, 510, 954
MaeI CTAG 2 cut(s) 429, 699
MaeII ACGT 2 cut(s) 56, 301
MaeIII GTNAC 2 cut(s) 212, 914
MalI GATC 7 cut(s) 201, 296, 480, 762, 770, 864, 940
MboI GATC 7 cut(s) 199, 294, 478, 760, 768, 862, 938
MboII GAAGA 4 cut(s) 317, 378, 717, 857
MflI RGATCY 3 cut(s) 294, 760, 768
MhlI GDGCHC 3 cut(s) 382, 643, 861
MluCI AATT 6 cut(s) 137, 195, 343, 368, 681, 811
MlyI GAGTC 2 cut(s) 158, 717
MmeI TCCRAC 1 cut(s) 22
MnlI CCTC 6 cut(s) 55, 72, 128, 194, 396, 797
Mph1103I ATGCAT 2 cut(s) 278, 805
MroXI GAANNNNTTC 1 cut(s) 803
MseI TTAA 4 cut(s) 93, 305, 492, 651
MslI CAYNNNNRTG 2 cut(s) 254, 634
MspI CCGG 2 cut(s) 909, 961
MspR9I CCNGG 3 cut(s) 311, 773, 910
MssI GTTTAAAC 1 cut(s) 306
Mva1269I GAATGC 2 cut(s) 803, 805
MvaI CCWGG 2 cut(s) 311, 773
MwoI GCNNNNNNNGC 1 cut(s) 88
NciI CCSGG 1 cut(s) 910
NcoI CCATGG 1 cut(s) 144
NdeII GATC 7 cut(s) 199, 294, 478, 760, 768, 862, 938
NlaIII CATG 3 cut(s) 132, 148, 715
NlaIV GGNNCC 3 cut(s) 19, 380, 762
NmuCI GTSAC 1 cut(s) 914
NsiI ATGCAT 2 cut(s) 278, 805
PctI GAATGC 2 cut(s) 803, 805
PdmI GAANNNNTTC 1 cut(s) 803
PfeI GAWTC 2 cut(s) 350, 470
PfoI TCCNGGA 1 cut(s) 771
PkrI GCNGC 2 cut(s) 577, 580
PleI GAGTC 2 cut(s) 157, 716
PmeI GTTTAAAC 1 cut(s) 306
PpsI GAGTC 2 cut(s) 157, 716
Psp6I CCWGG 2 cut(s) 309, 771
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 2 cut(s) 309, 771
PspN4I GGNNCC 3 cut(s) 19, 380, 762
PspOMI GGGCCC 1 cut(s) 378
PspPI GGNCC 2 cut(s) 378, 379
PsuI RGATCY 3 cut(s) 294, 760, 768
RsaI GTAC 3 cut(s) 242, 693, 717
RsaNI GTAC 3 cut(s) 241, 692, 716
RseI CAYNNNNRTG 2 cut(s) 254, 634
SaqAI TTAA 4 cut(s) 93, 305, 492, 651
SatI GCNGC 2 cut(s) 576, 579
Sau3AI GATC 7 cut(s) 199, 294, 478, 760, 768, 862, 938
Sau96I GGNCC 2 cut(s) 378, 379
SchI GAGTC 2 cut(s) 158, 717
ScrFI CCNGG 3 cut(s) 311, 773, 910
SduI GDGCHC 3 cut(s) 382, 643, 861
SfaNI GCATC 3 cut(s) 415, 510, 954
SfcI CTRYAG 1 cut(s) 442
SmiMI CAYNNNNRTG 2 cut(s) 254, 634
Sse9I AATT 6 cut(s) 137, 195, 343, 368, 681, 811
SsiI CCGC 1 cut(s) 579
SspMI CTAG 2 cut(s) 429, 699
StyD4I CCNGG 3 cut(s) 309, 771, 908
StyI CCWWGG 3 cut(s) 32, 144, 853
TaaI ACNGT 4 cut(s) 27, 48, 443, 748
TaiI ACGT 2 cut(s) 59, 304
TaqI TCGA 1 cut(s) 861
TasI AATT 6 cut(s) 137, 195, 343, 368, 681, 811
TatI WGTACW 2 cut(s) 240, 691
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 2 cut(s) 350, 470
Tru1I TTAA 4 cut(s) 93, 305, 492, 651
Tru9I TTAA 4 cut(s) 93, 305, 492, 651
TscAI CASTG 3 cut(s) 259, 335, 796
TseFI GTSAC 1 cut(s) 914
TseI GCWGC 1 cut(s) 575
Tsp45I GTSAC 1 cut(s) 914
TspDTI ATGAA 1 cut(s) 149
TspRI CASTG 3 cut(s) 259, 335, 796
XapI RAATTY 1 cut(s) 811
XmnI GAANNNNTTC 1 cut(s) 803
XspI CTAG 2 cut(s) 429, 699
Zsp2I ATGCAT 2 cut(s) 278, 805
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.