pycom07g13010

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005577
Physical Location & Seq
Forward (+)
14504319 .. 14505550
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g13010.1

Sequence Viewer

Length: 534 bp
ATGACTGAGTATGTTGTTACTCGTTGGTACCGTGCACCAGAATTGCTGCTTAATTGTTCGGAGTACACTGCAGCAATCGATATATGGTCTGTAGGTTGCATTCTAGGTGAAATCATGAACAGAAAACCCCTATTCCCTGGCAAAGATTATGTACATCAGCTGAGACTCATAACAGAGCTCTTAGGCTCACCTGATGACTCCAGCCTTGGATTTTTACGAAGTGATAATGCTCGAAGATATGTTCGACAACTACCTCAGTACCCAAAGCGGAGCTTCTCTCTTGGGTTTCCTGATATGTCTCCTAGCGCTATAGATTTGCTAGAGAAGATGCTTATCTTTGACCCAAACAGGCGTATTACAGTTGATGAGGCTCTTTGCCACCCATACTTGGCGCCTCTTCATGATATAAACGAGGAGCCTGTCTGCCCAATGCCTTTCAATTTTGATTTTGAGCAACCCTCGTTTACTGAAGAGAATATCAAGGAGCTCATCTGGAGAGAGTCCGTAAGGTTCAATCCAGATCCTATTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006971 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009504 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009861 GO:0009862 GO:0009863 GO:0009867 GO:0009868 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0014070 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031122 GO:0032260 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032870 GO:0033206 GO:0033993 GO:0035556 GO:0035690 GO:0035821 GO:0036211 GO:0042221 GO:0042493 GO:0042539 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0046677 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051321 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052306 GO:0052308 GO:0052552 GO:0052553 GO:0052564 GO:0052572 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071840 GO:0075136 GO:0080026 GO:0097305 GO:0097435 GO:0098542 GO:0140013 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1901701 GO:1902410 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04016 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04138 ko04139 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05206 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 ko05418 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04016 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04138 map04139 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05206 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

178

Amino Acids

20.64

Weight (kDa)

4.9

Isoelectric Point (pI)

68.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 130 3.1e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 27
AccB1I GGYRCC 2 cut(s) 27, 391
AciI CCGC 1 cut(s) 268
AclWI GGATC 1 cut(s) 515
AcuI CTGAAG 1 cut(s) 489
AcyI GRCGYC 1 cut(s) 392
AfaI GTAC 4 cut(s) 29, 65, 153, 260
AfeI AGCGCT 1 cut(s) 307
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 3 cut(s) 439, 514, 530
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 4 cut(s) 160, 178, 273, 487
AluI AGCT 4 cut(s) 160, 178, 273, 487
Alw21I GWGCWC 3 cut(s) 37, 180, 489
Alw26I GTCTC 2 cut(s) 157, 303
Alw44I GTGCAC 1 cut(s) 33
AlwI GGATC 1 cut(s) 515
Aor51HI AGCGCT 1 cut(s) 307
ApaLI GTGCAC 1 cut(s) 33
ApeKI GCWGC 2 cut(s) 46, 71
Asp718I GGTACC 1 cut(s) 27
AspLEI GCGC 2 cut(s) 308, 394
AsuHPI GGTGA 2 cut(s) 119, 180
BaeGI GKGCMC 1 cut(s) 37
BaeI ACNNNNGTAYC 2 cut(s) 242, 275
BanI GGYRCC 2 cut(s) 27, 391
BanII GRGCYC 2 cut(s) 180, 489
Bbv12I GWGCWC 3 cut(s) 37, 180, 489
BbvI GCAGC 2 cut(s) 33, 83
BciT130I CCWGG 1 cut(s) 138
BcoDI GTCTC 2 cut(s) 157, 303
BfaI CTAG 3 cut(s) 104, 303, 320
BfmI CTRYAG 3 cut(s) 69, 90, 309
BfoI RGCGCY 2 cut(s) 309, 395
BisI GCNGC 2 cut(s) 47, 72
BlsI GCNGC 2 cut(s) 48, 73
Bme1390I CCNGG 1 cut(s) 138
BmiI GGNNCC 3 cut(s) 29, 393, 417
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 1 cut(s) 318
BplI GAGNNNNNCTC 4 cut(s) 262, 294, 443, 475
BpmI CTGGAG 2 cut(s) 184, 514
Bsa29I ATCGAT 1 cut(s) 78
BsaBI GATNNNNATC 1 cut(s) 332
BsaHI GRCGYC 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 136, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse8I GATNNNNATC 1 cut(s) 332
BseBI CCWGG 1 cut(s) 138
BseCI ATCGAT 1 cut(s) 78
BseDI CCNNGG 2 cut(s) 136, 205
BseJI GATNNNNATC 1 cut(s) 332
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMII CTCAG 2 cut(s) 152, 269
BseRI GAGGAG 1 cut(s) 428
BseSI GKGCMC 1 cut(s) 37
BseXI GCAGC 2 cut(s) 33, 83
BshNI GGYRCC 2 cut(s) 27, 391
BshVI ATCGAT 1 cut(s) 78
BsiHKAI GWGCWC 3 cut(s) 37, 180, 489
BslI CCNNNNNNNGG 1 cut(s) 388
BsmAI GTCTC 2 cut(s) 157, 303
BsmI GAATGC 1 cut(s) 99
Bsp1286I GDGCHC 3 cut(s) 37, 180, 489
Bsp1407I TGTACA 1 cut(s) 151
Bsp143I GATC 1 cut(s) 520
BspACI CCGC 1 cut(s) 268
BspCNI CTCAG 2 cut(s) 153, 268
BspDI ATCGAT 1 cut(s) 78
BspHI TCATGA 2 cut(s) 114, 400
BspLI GGNNCC 3 cut(s) 29, 393, 417
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 1 cut(s) 515
BspT107I GGYRCC 2 cut(s) 27, 391
BsrGI TGTACA 1 cut(s) 151
BssECI CCNNGG 2 cut(s) 136, 205
BssMI GATC 1 cut(s) 520
BssNI GRCGYC 1 cut(s) 392
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 2 cut(s) 32, 361
Bst6I CTCTTC 2 cut(s) 402, 465
BstACI GRCGYC 1 cut(s) 392
BstAUI TGTACA 1 cut(s) 151
BstDEI CTNAG 4 cut(s) 6, 161, 181, 255
BstH2I RGCGCY 2 cut(s) 309, 395
BstHHI GCGC 2 cut(s) 308, 394
BstKTI GATC 1 cut(s) 523
BstMAI GTCTC 2 cut(s) 157, 303
BstMBI GATC 1 cut(s) 520
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstSFI CTRYAG 3 cut(s) 69, 90, 309
BstSLI GKGCMC 1 cut(s) 37
BstV1I GCAGC 2 cut(s) 33, 83
BstX2I RGATCY 1 cut(s) 520
BstYI RGATCY 1 cut(s) 520
Bsu15I ATCGAT 1 cut(s) 78
BsuTUI ATCGAT 1 cut(s) 78
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
CciI TCATGA 2 cut(s) 114, 400
CfoI GCGC 2 cut(s) 308, 394
ClaI ATCGAT 1 cut(s) 78
Csp6I GTAC 4 cut(s) 28, 64, 152, 259
CviAII CATG 2 cut(s) 115, 401
CviJI RGCY 8 cut(s) 160, 178, 186, 204, 273, 371, 418, 487
CviKI_1 RGCY 8 cut(s) 160, 178, 186, 204, 273, 371, 418, 487
CviQI GTAC 4 cut(s) 28, 64, 152, 259
DdeI CTNAG 4 cut(s) 6, 161, 181, 255
DinI GGCGCC 1 cut(s) 393
DpnI GATC 1 cut(s) 522
DpnII GATC 1 cut(s) 520
Eam1104I CTCTTC 2 cut(s) 402, 465
EarI CTCTTC 2 cut(s) 402, 465
Ecl136II GAGCTC 2 cut(s) 178, 487
Eco130I CCWWGG 1 cut(s) 205
Eco24I GRGCYC 2 cut(s) 180, 489
Eco47III AGCGCT 1 cut(s) 307
Eco53kI GAGCTC 2 cut(s) 178, 487
Eco57I CTGAAG 1 cut(s) 489
EcoICRI GAGCTC 2 cut(s) 178, 487
EcoRII CCWGG 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 205
EcoT38I GRGCYC 2 cut(s) 180, 489
EgeI GGCGCC 1 cut(s) 393
EheI GGCGCC 1 cut(s) 393
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 2 cut(s) 118, 404
FalI AAGNNNNNCTT 2 cut(s) 257, 289
FatI CATG 2 cut(s) 114, 400
Fnu4HI GCNGC 2 cut(s) 47, 72
FriOI GRGCYC 2 cut(s) 180, 489
Fsp4HI GCNGC 2 cut(s) 47, 72
FspBI CTAG 3 cut(s) 104, 303, 320
GlaI GCGC 2 cut(s) 307, 393
GluI GCNGC 2 cut(s) 47, 72
GsuI CTGGAG 2 cut(s) 184, 514
HaeII RGCGCY 2 cut(s) 309, 395
HhaI GCGC 2 cut(s) 308, 394
Hin1I GRCGYC 1 cut(s) 392
Hin1II CATG 2 cut(s) 118, 404
Hin6I GCGC 2 cut(s) 306, 392
HinP1I GCGC 2 cut(s) 306, 392
HinfI GANTC 3 cut(s) 165, 197, 500
HphI GGTGA 2 cut(s) 119, 180
Hpy166II GTNNAC 3 cut(s) 35, 66, 465
Hpy188I TCNGA 1 cut(s) 61
Hpy188III TCNNGA 5 cut(s) 115, 290, 401, 493, 518
Hpy8I GTNNAC 3 cut(s) 35, 66, 465
HpyCH4III ACNGT 2 cut(s) 32, 361
HpyCH4V TGCA 3 cut(s) 35, 71, 99
HpyF3I CTNAG 4 cut(s) 6, 161, 181, 255
Hsp92I GRCGYC 1 cut(s) 392
Hsp92II CATG 2 cut(s) 118, 404
HspAI GCGC 2 cut(s) 306, 392
KasI GGCGCC 1 cut(s) 391
KpnI GGTACC 1 cut(s) 31
Kzo9I GATC 1 cut(s) 520
LmnI GCTCC 3 cut(s) 270, 415, 484
LpnPI CCDG 9 cut(s) 51, 123, 150, 204, 214, 303, 334, 432, 478
Lsp1109I GCAGC 2 cut(s) 33, 83
LweI GCATC 1 cut(s) 318
MaeI CTAG 3 cut(s) 104, 303, 320
MaeIII GTNAC 1 cut(s) 16
MalI GATC 1 cut(s) 522
MboI GATC 1 cut(s) 520
MboII GAAGA 4 cut(s) 246, 337, 389, 482
MflI RGATCY 1 cut(s) 520
MhlI GDGCHC 3 cut(s) 37, 180, 489
MluCI AATT 3 cut(s) 41, 52, 439
Mly113I GGCGCC 1 cut(s) 392
MlyI GAGTC 3 cut(s) 159, 191, 509
MnlI CCTC 5 cut(s) 264, 361, 405, 406, 469
MseI TTAA 1 cut(s) 51
MspA1I CMGCKG 1 cut(s) 160
MspR9I CCNGG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 99
MvaI CCWGG 1 cut(s) 138
NarI GGCGCC 1 cut(s) 392
NdeII GATC 1 cut(s) 520
NlaIII CATG 2 cut(s) 118, 404
NlaIV GGNNCC 3 cut(s) 29, 393, 417
PagI TCATGA 2 cut(s) 114, 400
PcsI WCGNNNNNNNCGW 1 cut(s) 28
PctI GAATGC 1 cut(s) 99
PkrI GCNGC 2 cut(s) 48, 73
PleI GAGTC 3 cut(s) 159, 191, 508
PluTI GGCGCC 1 cut(s) 395
PpsI GAGTC 3 cut(s) 159, 191, 508
Psp124BI GAGCTC 2 cut(s) 180, 489
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 3 cut(s) 29, 393, 417
PstI CTGCAG 1 cut(s) 73
PsuI RGATCY 1 cut(s) 520
PvuII CAGCTG 1 cut(s) 160
RsaI GTAC 4 cut(s) 29, 65, 153, 260
RsaNI GTAC 4 cut(s) 28, 64, 152, 259
SacI GAGCTC 2 cut(s) 180, 489
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 2 cut(s) 47, 72
Sau3AI GATC 1 cut(s) 520
SchI GAGTC 3 cut(s) 159, 191, 509
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 3 cut(s) 37, 180, 489
SetI ASST 9 cut(s) 97, 109, 162, 180, 193, 256, 275, 489, 512
SfaNI GCATC 1 cut(s) 318
SfcI CTRYAG 3 cut(s) 69, 90, 309
SfoI GGCGCC 1 cut(s) 393
Sse9I AATT 3 cut(s) 41, 52, 439
SsiI CCGC 1 cut(s) 268
SspDI GGCGCC 1 cut(s) 391
SspMI CTAG 3 cut(s) 104, 303, 320
SstI GAGCTC 2 cut(s) 180, 489
StyD4I CCNGG 1 cut(s) 136
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 32, 361
TaqI TCGA 3 cut(s) 78, 232, 244
TasI AATT 3 cut(s) 41, 52, 439
TatI WGTACW 2 cut(s) 63, 151
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 1 cut(s) 73
TseI GCWGC 2 cut(s) 46, 71
TspDTI ATGAA 2 cut(s) 131, 389
TspGWI ACGGA 1 cut(s) 493
TspRI CASTG 1 cut(s) 73
VneI GTGCAC 1 cut(s) 33
XspI CTAG 3 cut(s) 104, 303, 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.