pycom07g13000

mitogen-activated protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005577
Physical Location & Seq
Forward (+)
14500720 .. 14504227
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g13000.1

Sequence Viewer

Length: 924 bp
ATGATATCATCTACGTTAAGGGGAAGAGATAAAACAATACTTATCGCTTATCGAGATTCAAAGCACATAACATCAAGATGCCACACCAAAACCTCACATAAAGAAGAGTTAAAAAGTCCATTTCTTTCACGGGTCACCCAAGTTTTTTCCAGTTCAAAAGCCCTCCTTGAAAGCTCTCGCTCTCTCTCTCTCTCTCTCTCCCTCTCTCCCCCCTTACACACTACTCCCCAGATTTCTTTGGAATTTTCCAAAAACCCCAAAAAGAAAGAAAGAAAGAAATGTGAAAACCTTTTCAAATTACAGCTACCGACACCCCTCCAAAAATTTTGCATTAGCAGCAGCTCGCCTCCTCCATTACCTCTAAAGCTCCTCCTCCGCAGCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCATCGGACCCCCCGGGTTGACCATGGAGTCCAGCGCTGCTCCGGCTGGTGACCACAACATCAGAGGGGTGCCGACCCACGGCGGACGCTACGTTCAGTACAATGTGTATGGCAACCTCTTTGAAGTTTCCAGGAAGTATGTCCCGCCCATTAGGCCCGTCGGCAGAGGGGCTTATGGTATCGTCTGTGCTGCTGTCAACGCTGAGACTCGTGAGGAGGTTGCCATTAAGAAGATTGGTAATGCATTTGACAACAGAATAGATGCCAAAAGGACTTTACGAGAAATTAAACTTCTTCGGCACATGGATCATGAAAATGTTATTGCCATCAAAGACATCATACGGCCTCCACAGAAGGAGAACTTCAATGATGTCTACATTGTTTATGAATTAATGGACACTGATCTTCATCAGATTATACGGTCCAACCAACCATTATTTTATTTGTTAGTACTTTCTGTACCAGTTGTTACGAGGGCTCAAATATGTACATTCAGCAGGTGTTTTACATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000226 GO:0000278 GO:0000280 GO:0000281 GO:0000910 GO:0000911 GO:0001101 GO:0002376 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004707 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005856 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006971 GO:0006972 GO:0006996 GO:0007010 GO:0007017 GO:0007049 GO:0007112 GO:0007140 GO:0007154 GO:0007165 GO:0007275 GO:0007276 GO:0008150 GO:0008152 GO:0009266 GO:0009409 GO:0009504 GO:0009555 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009627 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009733 GO:0009737 GO:0009751 GO:0009753 GO:0009755 GO:0009814 GO:0009861 GO:0009862 GO:0009863 GO:0009867 GO:0009868 GO:0009987 GO:0010033 GO:0010243 GO:0010468 GO:0014070 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0019953 GO:0022402 GO:0022412 GO:0022414 GO:0023014 GO:0023052 GO:0030865 GO:0031122 GO:0032260 GO:0032501 GO:0032502 GO:0032504 GO:0032506 GO:0032870 GO:0033206 GO:0033993 GO:0035556 GO:0035690 GO:0035821 GO:0036211 GO:0042221 GO:0042493 GO:0042539 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043622 GO:0044003 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044403 GO:0044419 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045087 GO:0046677 GO:0048229 GO:0048232 GO:0048285 GO:0048609 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051301 GO:0051321 GO:0051701 GO:0051704 GO:0051707 GO:0051716 GO:0051817 GO:0052031 GO:0052167 GO:0052169 GO:0052173 GO:0052200 GO:0052255 GO:0052306 GO:0052308 GO:0052552 GO:0052553 GO:0052564 GO:0052572 GO:0060255 GO:0061640 GO:0065007 GO:0070887 GO:0071229 GO:0071236 GO:0071310 GO:0071395 GO:0071407 GO:0071446 GO:0071495 GO:0071704 GO:0071840 GO:0075136 GO:0080026 GO:0097305 GO:0097435 GO:0098542 GO:0140013 GO:0140096 GO:1901564 GO:1901698 GO:1901700 GO:1901701 GO:1902410 GO:1903046 GO:1903047
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko01524 ko04010 ko04011 ko04012 ko04013 ko04014 ko04015 ko04016 ko04022 ko04024 ko04062 ko04066 ko04068 ko04071 ko04072 ko04114 ko04138 ko04139 ko04140 ko04150 ko04151 ko04210 ko04214 ko04218 ko04261 ko04270 ko04320 ko04350 ko04360 ko04370 ko04371 ko04380 ko04510 ko04520 ko04540 ko04550 ko04611 ko04620 ko04621 ko04650 ko04657 ko04658 ko04659 ko04660 ko04662 ko04664 ko04666 ko04668 ko04713 ko04720 ko04722 ko04723 ko04724 ko04725 ko04726 ko04730 ko04810 ko04910 ko04912 ko04914 ko04915 ko04916 ko04917 ko04919 ko04921 ko04926 ko04930 ko04933 ko04934 ko04960 ko05010 ko05020 ko05034 ko05131 ko05132 ko05133 ko05140 ko05142 ko05145 ko05152 ko05160 ko05161 ko05164 ko05165 ko05167 ko05200 ko05203 ko05205 ko05206 ko05210 ko05211 ko05212 ko05213 ko05214 ko05215 ko05216 ko05218 ko05219 ko05220 ko05221 ko05223 ko05224 ko05225 ko05226 ko05230 ko05231 ko05418 map01521 map01522 map01524 map04010 map04011 map04012 map04013 map04014 map04015 map04016 map04022 map04024 map04062 map04066 map04068 map04071 map04072 map04114 map04138 map04139 map04140 map04150 map04151 map04210 map04214 map04218 map04261 map04270 map04320 map04350 map04360 map04370 map04371 map04380 map04510 map04520 map04540 map04550 map04611 map04620 map04621 map04650 map04657 map04658 map04659 map04660 map04662 map04664 map04666 map04668 map04713 map04720 map04722 map04723 map04724 map04725 map04726 map04730 map04810 map04910 map04912 map04914 map04915 map04916 map04917 map04919 map04921 map04926 map04930 map04933 map04934 map04960 map05010 map05020 map05034 map05131 map05132 map05133 map05140 map05142 map05145 map05152 map05160 map05161 map05164 map05165 map05167 map05200 map05203 map05205 map05206 map05210 map05211 map05212 map05213 map05214 map05215 map05216 map05218 map05219 map05220 map05221 map05223 map05224 map05225 map05226 map05230 map05231 map05418
Pfam Domains
Protein Families
Pkinase

Protein Analysis

308

Amino Acids

34.48

Weight (kDa)

9.84

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 188 - 280 3.9e-15 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 188 - 280 2e-08 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 897
AasI GACNNNNNNGTC 1 cut(s) 436
Acc36I ACCTGC 1 cut(s) 897
AccB1I GGYRCC 1 cut(s) 478
AccI GTMKAC 1 cut(s) 783
AciI CCGC 3 cut(s) 376, 492, 554
AclWI GGATC 1 cut(s) 723
AcsI RAATTY 2 cut(s) 242, 323
AfaI GTAC 4 cut(s) 509, 861, 870, 898
AfeI AGCGCT 1 cut(s) 445
AfiI CCNNNNNNNGG 1 cut(s) 488
AgsI TTSAA 6 cut(s) 60, 156, 170, 295, 533, 775
AjnI CCWGG 1 cut(s) 539
AloI GAACNNNNNNTCC 2 cut(s) 486, 518
AluBI AGCT 5 cut(s) 174, 304, 342, 367, 381
AluI AGCT 5 cut(s) 174, 304, 342, 367, 381
Alw26I GTCTC 1 cut(s) 608
AlwI GGATC 1 cut(s) 723
Ama87I CYCGRG 1 cut(s) 422
Aor51HI AGCGCT 1 cut(s) 445
AoxI GGCC 2 cut(s) 563, 752
ApeKI GCWGC 5 cut(s) 336, 339, 378, 446, 599
ApoI RAATTY 2 cut(s) 242, 323
AseI ATTAAT 1 cut(s) 800
AspLEI GCGC 1 cut(s) 446
AspS9I GGNCC 3 cut(s) 416, 564, 831
AsuC2I CCSGG 2 cut(s) 423, 424
AsuHPI GGTGA 2 cut(s) 127, 470
AvaI CYCGRG 1 cut(s) 422
AvaII GGWCC 2 cut(s) 416, 831
BanI GGYRCC 1 cut(s) 478
BanII GRGCYC 1 cut(s) 889
BauI CACGAG 1 cut(s) 618
BbvI GCAGC 5 cut(s) 348, 351, 390, 433, 586
BccI CCATC 1 cut(s) 743
BceAI ACGGC 2 cut(s) 505, 767
BciT130I CCWGG 1 cut(s) 541
BcnI CCSGG 2 cut(s) 423, 424
BcoDI GTCTC 1 cut(s) 608
BfoI RGCGCY 1 cut(s) 447
BfuAI ACCTGC 1 cut(s) 897
BglI GCCNNNNNGGC 1 cut(s) 562
BisI GCNGC 5 cut(s) 337, 340, 379, 447, 600
BlsI GCNGC 5 cut(s) 338, 341, 380, 448, 601
BmcAI AGTACT 1 cut(s) 861
Bme1390I CCNGG 3 cut(s) 423, 424, 541
Bme18I GGWCC 2 cut(s) 416, 831
BmeT110I CYCGRG 1 cut(s) 422
BmgT120I GGNCC 3 cut(s) 416, 564, 831
BmiI GGNNCC 2 cut(s) 418, 480
BmrFI CCNGG 3 cut(s) 423, 424, 541
BmsI GCATC 2 cut(s) 68, 661
BpuMI CCSGG 2 cut(s) 423, 424
BsaBI GATNNNNATC 1 cut(s) 816
BsaJI CCNNGG 4 cut(s) 421, 422, 432, 487
Bsc4I CCNNNNNNNGG 1 cut(s) 488
Bse1I ACTGG 2 cut(s) 150, 872
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 4 cut(s) 421, 422, 432, 487
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 1 cut(s) 488
BseMII CTCAG 1 cut(s) 603
BseNI ACTGG 2 cut(s) 150, 872
BseRI GAGGAG 4 cut(s) 339, 359, 362, 638
BseXI GCAGC 5 cut(s) 348, 351, 390, 433, 586
BshFI GGCC 2 cut(s) 565, 754
BshNI GGYRCC 1 cut(s) 478
BsiHKCI CYCGRG 1 cut(s) 422
BsiSI CCGG 2 cut(s) 423, 452
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 1 cut(s) 488
BsmAI GTCTC 1 cut(s) 608
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 2 cut(s) 565, 754
BsoBI CYCGRG 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 889
Bsp1407I TGTACA 1 cut(s) 896
Bsp143I GATC 2 cut(s) 715, 811
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 3 cut(s) 376, 492, 554
BspANI GGCC 2 cut(s) 565, 754
BspCNI CTCAG 1 cut(s) 604
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 2 cut(s) 418, 480
BspMI ACCTGC 1 cut(s) 897
BspPI GGATC 1 cut(s) 723
BspT107I GGYRCC 1 cut(s) 478
BsrGI TGTACA 1 cut(s) 896
BsrI ACTGG 2 cut(s) 150, 872
BssECI CCNNGG 4 cut(s) 421, 422, 432, 487
BssMI GATC 2 cut(s) 715, 811
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 1 cut(s) 432
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 1 cut(s) 831
Bst6I CTCTTC 2 cut(s) 19, 99
BstAUI TGTACA 1 cut(s) 896
BstC8I GCNNGC 1 cut(s) 344
BstDEI CTNAG 1 cut(s) 612
BstDSI CCRYGG 2 cut(s) 432, 487
BstEII GGTNACC 2 cut(s) 133, 458
BstH2I RGCGCY 1 cut(s) 447
BstHHI GCGC 1 cut(s) 446
BstKTI GATC 2 cut(s) 718, 814
BstMAI GTCTC 1 cut(s) 608
BstMBI GATC 2 cut(s) 715, 811
BstMWI GCNNNNNNNGC 4 cut(s) 336, 452, 562, 608
BstNI CCWGG 1 cut(s) 541
BstPI GGTNACC 2 cut(s) 133, 458
BstSCI CCNGG 3 cut(s) 421, 422, 539
BstV1I GCAGC 5 cut(s) 348, 351, 390, 433, 586
BsuRI GGCC 2 cut(s) 565, 754
BtgI CCRYGG 2 cut(s) 432, 487
BtsIMutI CAGTG 1 cut(s) 807
BveI ACCTGC 1 cut(s) 897
Cac8I GCNNGC 1 cut(s) 344
CciI TCATGA 1 cut(s) 718
CfoI GCGC 1 cut(s) 446
Cfr13I GGNCC 3 cut(s) 416, 564, 831
Cfr9I CCCGGG 1 cut(s) 422
CseI GACGC 1 cut(s) 504
Csp6I GTAC 4 cut(s) 508, 860, 869, 897
CviAII CATG 3 cut(s) 433, 712, 719
CviQI GTAC 4 cut(s) 508, 860, 869, 897
DdeI CTNAG 1 cut(s) 612
DpnI GATC 2 cut(s) 717, 813
DpnII GATC 2 cut(s) 715, 811
DrdI GACNNNNNNGTC 1 cut(s) 436
DseDI GACNNNNNNGTC 1 cut(s) 436
Eam1104I CTCTTC 2 cut(s) 19, 99
EarI CTCTTC 2 cut(s) 19, 99
EciI GGCGGA 1 cut(s) 507
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 889
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 2 cut(s) 416, 831
Eco47III AGCGCT 1 cut(s) 445
Eco88I CYCGRG 1 cut(s) 422
Eco91I GGTNACC 2 cut(s) 133, 458
EcoO65I GGTNACC 2 cut(s) 133, 458
EcoRII CCWGG 1 cut(s) 539
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 1 cut(s) 432
EcoT22I ATGCAT 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 889
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 3 cut(s) 436, 715, 722
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 755, 787
FaqI GGGAC 1 cut(s) 536
FatI CATG 3 cut(s) 432, 711, 718
FauI CCCGC 1 cut(s) 561
FblI GTMKAC 1 cut(s) 783
Fnu4HI GCNGC 5 cut(s) 337, 340, 379, 447, 600
FriOI GRGCYC 1 cut(s) 889
Fsp4HI GCNGC 5 cut(s) 337, 340, 379, 447, 600
GlaI GCGC 1 cut(s) 445
GluI GCNGC 5 cut(s) 337, 340, 379, 447, 600
HaeII RGCGCY 1 cut(s) 447
HaeIII GGCC 2 cut(s) 565, 754
HapII CCGG 2 cut(s) 423, 452
HgaI GACGC 1 cut(s) 504
HhaI GCGC 1 cut(s) 446
Hin1II CATG 3 cut(s) 436, 715, 722
Hin6I GCGC 1 cut(s) 444
HinP1I GCGC 1 cut(s) 444
HincII GTYRAC 2 cut(s) 429, 607
HindII GTYRAC 2 cut(s) 429, 607
HinfI GANTC 3 cut(s) 56, 437, 616
HpaII CCGG 2 cut(s) 423, 452
HphI GGTGA 2 cut(s) 127, 470
Hpy166II GTNNAC 3 cut(s) 429, 607, 784
Hpy188I TCNGA 3 cut(s) 416, 473, 822
Hpy188III TCNNGA 5 cut(s) 53, 75, 620, 719, 921
Hpy8I GTNNAC 3 cut(s) 429, 607, 784
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 1 cut(s) 757
HpyCH4III ACNGT 1 cut(s) 831
HpyCH4IV ACGT 2 cut(s) 14, 501
HpyCH4V TGCA 2 cut(s) 330, 653
HpyF10VI GCNNNNNNNGC 4 cut(s) 336, 452, 562, 608
HpyF3I CTNAG 1 cut(s) 612
HpySE526I ACGT 2 cut(s) 14, 501
Hsp92II CATG 3 cut(s) 436, 715, 722
HspAI GCGC 1 cut(s) 444
Kzo9I GATC 2 cut(s) 715, 811
LmnI GCTCC 2 cut(s) 372, 454
Lsp1109I GCAGC 5 cut(s) 348, 351, 390, 433, 586
LweI GCATC 2 cut(s) 68, 661
MaeII ACGT 2 cut(s) 14, 501
MaeIII GTNAC 3 cut(s) 133, 458, 877
MalI GATC 2 cut(s) 717, 813
MboI GATC 2 cut(s) 715, 811
MboII GAAGA 5 cut(s) 36, 116, 652, 695, 806
MhlI GDGCHC 1 cut(s) 889
MluCI AATT 5 cut(s) 242, 296, 323, 693, 797
MlyI GAGTC 2 cut(s) 446, 610
MmeI TCCRAC 1 cut(s) 858
Mph1103I ATGCAT 1 cut(s) 655
MseI TTAA 5 cut(s) 17, 110, 636, 696, 800
MslI CAYNNNNRTG 2 cut(s) 76, 723
MspI CCGG 2 cut(s) 423, 452
MspR9I CCNGG 3 cut(s) 423, 424, 541
MvaI CCWGG 1 cut(s) 541
MwoI GCNNNNNNNGC 4 cut(s) 336, 452, 562, 608
NciI CCSGG 2 cut(s) 423, 424
NcoI CCATGG 1 cut(s) 432
NdeII GATC 2 cut(s) 715, 811
NlaIII CATG 3 cut(s) 436, 715, 722
NlaIV GGNNCC 2 cut(s) 418, 480
NmuCI GTSAC 2 cut(s) 133, 458
NsiI ATGCAT 1 cut(s) 655
PagI TCATGA 1 cut(s) 718
PaqCI CACCTGC 1 cut(s) 897
PfeI GAWTC 1 cut(s) 56
PfoI TCCNGGA 1 cut(s) 539
PkrI GCNGC 5 cut(s) 338, 341, 380, 448, 601
PleI GAGTC 2 cut(s) 445, 610
PpsI GAGTC 2 cut(s) 445, 610
PshBI ATTAAT 1 cut(s) 800
Psp6I CCWGG 1 cut(s) 539
PspEI GGTNACC 2 cut(s) 133, 458
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 2 cut(s) 418, 480
PspPI GGNCC 3 cut(s) 416, 564, 831
RsaI GTAC 4 cut(s) 509, 861, 870, 898
RsaNI GTAC 4 cut(s) 508, 860, 869, 897
RseI CAYNNNNRTG 2 cut(s) 76, 723
SaqAI TTAA 5 cut(s) 17, 110, 636, 696, 800
SatI GCNGC 5 cut(s) 337, 340, 379, 447, 600
Sau3AI GATC 2 cut(s) 715, 811
Sau96I GGNCC 3 cut(s) 416, 564, 831
ScaI AGTACT 1 cut(s) 861
SchI GAGTC 2 cut(s) 446, 610
ScrFI CCNGG 3 cut(s) 423, 424, 541
SduI GDGCHC 1 cut(s) 889
SfaNI GCATC 2 cut(s) 68, 661
SinI GGWCC 2 cut(s) 416, 831
SmaI CCCGGG 1 cut(s) 424
SmiMI CAYNNNNRTG 2 cut(s) 76, 723
Sse9I AATT 5 cut(s) 242, 296, 323, 693, 797
SsiI CCGC 3 cut(s) 376, 492, 554
StyD4I CCNGG 3 cut(s) 421, 422, 539
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 831
TaiI ACGT 2 cut(s) 17, 504
TaqI TCGA 1 cut(s) 52
TasI AATT 5 cut(s) 242, 296, 323, 693, 797
TatI WGTACW 3 cut(s) 507, 859, 896
TfiI GAWTC 1 cut(s) 56
Tru1I TTAA 5 cut(s) 17, 110, 636, 696, 800
Tru9I TTAA 5 cut(s) 17, 110, 636, 696, 800
TscAI CASTG 1 cut(s) 814
TseFI GTSAC 2 cut(s) 133, 458
TseI GCWGC 5 cut(s) 336, 339, 378, 446, 599
Tsp45I GTSAC 2 cut(s) 133, 458
TspDTI ATGAA 3 cut(s) 735, 806, 810
TspMI CCCGGG 1 cut(s) 422
TspRI CASTG 1 cut(s) 814
VpaK11BI GGWCC 2 cut(s) 416, 831
VspI ATTAAT 1 cut(s) 800
XapI RAATTY 2 cut(s) 242, 323
XmaI CCCGGG 1 cut(s) 422
XmiI GTMKAC 1 cut(s) 783
ZrmI AGTACT 1 cut(s) 861
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.