Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0015431

Physical Location & Seq

Genomic Coordinates Reverse (-)

17367139 .. 17370985

Gene Structure 3847 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1069600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGGGCACTAATCTGATAGCATTCGGAGGCGGTGGAGGAATAGCAGTAGGCGGAGGAGTGAGAGTTTCAATGGCGACACCGTGGGCTGTGAGAGCAGAACTCCCCAAAGCTCCTGACCTGTGCGTTGACCGCTCCATTCTGGGAAGTGGGTCTTCCTCGTCTGGCCGTCATGACCTCGTTTTCGTCGTCAATCCTAGCAGTGCGAATGGGAGGACAGGGAAGGAGTGGAAGAAGCTGCTTCCATATCTAAGGTCTCGCCTTGGTGCTGATTGCAACATATGTGAATCTTTAACATCAGGCCCGTCTCATGCAATTGACATAACGAGGGAGGCTATACGTGAGGGAGCTGATGCTGTAATCGCAGTGGGAGGAGATGGAACTCTGCATGAGGTTGTTAATGGATTCTTTTGGGCAGGAAAAACTGTTACTAATTATGATAGGGATGCCACCCATTCAACTGCACTTGGTCTCATTCCTTTGGGGACTGGATCCGATTTTGCCAGAACATTTGGCTGGAAAAATGATCCCCATGAAGCCATTGACCGTATAGCCAAAGGGCAGAGATCACAGATTGATGTTGGTGTTATTAGTGGAGAAGATGGAGAACCTCATTACTTTGCTAATGTTGCTGACATTCATTTGAGTGCAAAAGCAGGATTCTATGCTTCTAGTTATAAGAGATTTGGAAACTTGTGCTACGTTATTGGTTCATTAAAAGCTTTTGTTGGGCACTGTAATCAGGACCTTAAAATTAAGGTCAATGAAGGGGAGTGGGAAGTATACTCTCAAGTAACTGCCGTTTGTGTTGGAAATGCAAAATACTTTGGTGGTGGTATGAAAATTACACCAAATGCTGACCCTCGCAGCGGCAATTTTGAGGTTGTGATCCTTCAAGACTTCAAGTGGTATGACTTTATTCTGAAGCTACATAAGCTCTACAATGGGATGCATCTAACAATGAAAAATGTATCTTCAAGAAGCGTGCATTCTATTGAGGTGGAAGACATTTCAGGCAGTGGCAGCATTTATGTTCAATCTGACGGCGAACGTATAGGATTCCTGCCTAGGAAGTTTTGTATATTACCTTCTGCAATCGAAATGATATGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005886 GO:0006066 GO:0006629 GO:0006643 GO:0006664 GO:0006665 GO:0006671 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0009247 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017050 GO:0019751 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046467 GO:0046519 GO:0050896 GO:0071704 GO:0071944 GO:0097164 GO:1901135 GO:1901137 GO:1901564 GO:1901576 GO:1901615 GO:1903509

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DAGK_cat

Protein Analysis

370

Amino Acids

39.7

Weight (kDa)

6.83

Isoelectric Point (pI)

31.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	59 - 195	4.7e-29	Diacylglycerol kinase catalytic domain
YegS_C	PF19279	226 - 368	2.4e-24	YegS C-terminal NAD kinase beta sandwich-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015431)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46090
fragaria_vesca	FvH4_7g15100
malus_domestica	MD01G1069600.v1.1 MD07G1132800.v1.1
prunus_persica	Prupe.2G175700_v2.0.a1
pyrus_communis	pycom01g09890 pycom07g12960
rosa_chinensis	RchiOBHm_Chr1g0357291
rosa_laevigata	RLG00000028030
rosa_rugosa	Rorug01G0256100
rosa_samantha	Rh1AG269400 Rh1BG237300 Rh1CG253600 Rh1DG265200
rosa_wichuraiana	Rw1G023860 Rw1G024000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	675
AccBSI	CCGCTC	1 cut(s)	132
AccI	GTMKAC	1 cut(s)	780
AciI	CCGC	4 cut(s)	30, 51, 130, 867
AclWI	GGATC	4 cut(s)	483, 496, 518, 880
AcoI	YGGCCR	1 cut(s)	163
AcuI	CTGAAG	1 cut(s)	941
AfiI	CCNNNNNNNGG	1 cut(s)	866
AgsI	TTSAA	6 cut(s)	69, 456, 893, 901, 975, 1034
AluBI	AGCT	6 cut(s)	110, 235, 347, 719, 925, 934
AluI	AGCT	6 cut(s)	110, 235, 347, 719, 925, 934
Alw26I	GTCTC	3 cut(s)	258, 309, 473
AlwI	GGATC	4 cut(s)	483, 496, 518, 880
AoxI	GGCC	2 cut(s)	163, 298
ApeKI	GCWGC	3 cut(s)	235, 864, 1020
ArsI	GACNNNNNNTTYG	2 cut(s)	164, 196
AspA2I	CCTAGG	1 cut(s)	1064
AspS9I	GGNCC	2 cut(s)	299, 742
AvaII	GGWCC	1 cut(s)	742
AvrII	CCTAGG	1 cut(s)	1064
BaeGI	GKGCMC	2 cut(s)	8, 732
BamHI	GGATCC	1 cut(s)	488
BarI	GAAGNNNNNNTAC	2 cut(s)	1069, 1101
BbsI	GAAGAC	2 cut(s)	144, 1008
BbvI	GCAGC	3 cut(s)	222, 876, 1032
BccI	CCATC	2 cut(s)	368, 593
BceAI	ACGGC	3 cut(s)	150, 782, 1057
BcoDI	GTCTC	3 cut(s)	258, 309, 473
BfaI	CTAG	3 cut(s)	195, 669, 1065
BisI	GCNGC	4 cut(s)	236, 865, 868, 1021
BlnI	CCTAGG	1 cut(s)	1064
BlsI	GCNGC	4 cut(s)	237, 866, 869, 1022
Bme18I	GGWCC	1 cut(s)	742
BmgT120I	GGNCC	2 cut(s)	299, 742
BmiI	GGNNCC	1 cut(s)	490
BmsI	GCATC	4 cut(s)	340, 433, 936, 958
BpiI	GAAGAC	2 cut(s)	144, 1008
BplI	GAGNNNNNCTC	2 cut(s)	84, 116
BpuEI	CTTGAG	1 cut(s)	771
BsaAI	YACGTR	1 cut(s)	338
BsaI	GGTCTC	2 cut(s)	258, 473
BsaJI	CCNNGG	3 cut(s)	80, 259, 1064
BsaXI	ACNNNNNCTCC	2 cut(s)	48, 78
Bsc4I	CCNNNNNNNGG	1 cut(s)	866
Bse1I	ACTGG	1 cut(s)	490
BseDI	CCNNGG	3 cut(s)	80, 259, 1064
BseGI	GGATG	2 cut(s)	448, 951
BseLI	CCNNNNNNNGG	1 cut(s)	866
BseNI	ACTGG	1 cut(s)	490
BseRI	GAGGAG	2 cut(s)	69, 384
BseSI	GKGCMC	2 cut(s)	8, 732
BseXI	GCAGC	3 cut(s)	222, 876, 1032
BsgI	GTGCAG	1 cut(s)	444
BshFI	GGCC	2 cut(s)	165, 300
BslFI	GGGAC	1 cut(s)	496
BslI	CCNNNNNNNGG	1 cut(s)	866
BsmAI	GTCTC	3 cut(s)	258, 309, 473
BsmBI	CGTCTC	1 cut(s)	309
BsmFI	GGGAC	1 cut(s)	496
BsmI	GAATGC	2 cut(s)	20, 985
BsnI	GGCC	2 cut(s)	165, 300
Bso31I	GGTCTC	2 cut(s)	258, 473
Bsp1286I	GDGCHC	2 cut(s)	8, 732
Bsp143I	GATC	4 cut(s)	488, 523, 563, 885
BspACI	CCGC	4 cut(s)	30, 51, 130, 867
BspANI	GGCC	2 cut(s)	165, 300
BspHI	TCATGA	1 cut(s)	169
BspLI	GGNNCC	1 cut(s)	490
BspPI	GGATC	4 cut(s)	483, 496, 518, 880
BspTNI	GGTCTC	2 cut(s)	258, 473
BsrBI	CCGCTC	1 cut(s)	132
BsrI	ACTGG	1 cut(s)	490
BssECI	CCNNGG	3 cut(s)	80, 259, 1064
BssMI	GATC	4 cut(s)	488, 523, 563, 885
BssNAI	GTATAC	1 cut(s)	781
BssT1I	CCWWGG	2 cut(s)	259, 1064
Bst1107I	GTATAC	1 cut(s)	781
Bst4CI	ACNGT	4 cut(s)	81, 424, 545, 734
BstBAI	YACGTR	1 cut(s)	338
BstC8I	GCNNGC	1 cut(s)	983
BstDEI	CTNAG	1 cut(s)	248
BstDSI	CCRYGG	1 cut(s)	80
BstF5I	GGATG	2 cut(s)	448, 951
BstKTI	GATC	4 cut(s)	491, 526, 566, 888
BstMAI	GTCTC	3 cut(s)	258, 309, 473
BstMBI	GATC	4 cut(s)	488, 523, 563, 885
BstMWI	GCNNNNNNNGC	6 cut(s)	92, 129, 359, 626, 931, 1020
BstSLI	GKGCMC	2 cut(s)	8, 732
BstV1I	GCAGC	3 cut(s)	222, 876, 1032
BstV2I	GAAGAC	2 cut(s)	144, 1008
BstX2I	RGATCY	1 cut(s)	488
BstYI	RGATCY	1 cut(s)	488
BstZ17I	GTATAC	1 cut(s)	781
BsuRI	GGCC	2 cut(s)	165, 300
BtgI	CCRYGG	1 cut(s)	80
BtsCI	GGATG	2 cut(s)	448, 951
BtsI	GCAGTG	3 cut(s)	205, 369, 1021
BtsIMutI	CAGTG	4 cut(s)	205, 369, 730, 1021
Cac8I	GCNNGC	1 cut(s)	983
CciI	TCATGA	1 cut(s)	169
Cfr13I	GGNCC	2 cut(s)	299, 742
CviAII	CATG	4 cut(s)	170, 308, 386, 530
DdeI	CTNAG	1 cut(s)	248
DpnI	GATC	4 cut(s)	490, 525, 565, 887
DpnII	GATC	4 cut(s)	488, 523, 563, 885
EaeI	YGGCCR	1 cut(s)	163
EciI	GGCGGA	1 cut(s)	66
Eco130I	CCWWGG	2 cut(s)	259, 1064
Eco31I	GGTCTC	2 cut(s)	258, 473
Eco47I	GGWCC	1 cut(s)	742
Eco57I	CTGAAG	1 cut(s)	941
EcoO109I	RGGNCCY	1 cut(s)	742
EcoT14I	CCWWGG	2 cut(s)	259, 1064
EcoT22I	ATGCAT	1 cut(s)	951
ErhI	CCWWGG	2 cut(s)	259, 1064
Esp3I	CGTCTC	1 cut(s)	309
FaeI	CATG	4 cut(s)	173, 311, 389, 533
FalI	AAGNNNNNCTT	2 cut(s)	136, 168
FaqI	GGGAC	1 cut(s)	496
FatI	CATG	4 cut(s)	169, 307, 385, 529
FauNDI	CATATG	1 cut(s)	278
FblI	GTMKAC	1 cut(s)	780
Fnu4HI	GCNGC	4 cut(s)	236, 865, 868, 1021
FokI	GGATG	2 cut(s)	455, 958
Fsp4HI	GCNGC	4 cut(s)	236, 865, 868, 1021
FspBI	CTAG	3 cut(s)	195, 669, 1065
GluI	GCNGC	4 cut(s)	236, 865, 868, 1021
HaeIII	GGCC	2 cut(s)	165, 300
Hin1II	CATG	4 cut(s)	173, 311, 389, 533
HincII	GTYRAC	1 cut(s)	127
HindII	GTYRAC	1 cut(s)	127
HindIII	AAGCTT	1 cut(s)	717
HinfI	GANTC	4 cut(s)	284, 402, 657, 1056
Hpy166II	GTNNAC	2 cut(s)	127, 781
Hpy188I	TCNGA	5 cut(s)	15, 26, 493, 921, 1039
Hpy188III	TCNNGA	5 cut(s)	113, 170, 740, 893, 975
Hpy8I	GTNNAC	2 cut(s)	127, 781
Hpy99I	CGWCG	1 cut(s)	188
HpyAV	CCTTC	4 cut(s)	214, 758, 899, 1095
HpyCH4III	ACNGT	4 cut(s)	81, 424, 545, 734
HpyCH4IV	ACGT	3 cut(s)	337, 699, 1048
HpyCH4V	TGCA	9 cut(s)	273, 311, 385, 461, 647, 815, 949, 985, 1091
HpyF10VI	GCNNNNNNNGC	6 cut(s)	92, 129, 359, 626, 931, 1020
HpyF3I	CTNAG	1 cut(s)	248
HpySE526I	ACGT	3 cut(s)	337, 699, 1048
Hsp92II	CATG	4 cut(s)	173, 311, 389, 533
Kzo9I	GATC	4 cut(s)	488, 523, 563, 885
LmnI	GCTCC	3 cut(s)	115, 137, 344
Lsp1109I	GCAGC	3 cut(s)	222, 876, 1032
LweI	GCATC	4 cut(s)	340, 433, 936, 958
MaeI	CTAG	3 cut(s)	195, 669, 1065
MaeII	ACGT	3 cut(s)	337, 699, 1048
MaeIII	GTNAC	2 cut(s)	424, 790
MalI	GATC	4 cut(s)	490, 525, 565, 887
MbiI	CCGCTC	1 cut(s)	132
MboI	GATC	4 cut(s)	488, 523, 563, 885
MboII	GAAGA	5 cut(s)	144, 241, 608, 963, 1013
MfeI	CAATTG	1 cut(s)	312
MflI	RGATCY	1 cut(s)	488
MhlI	GDGCHC	2 cut(s)	8, 732
MluCI	AATT	5 cut(s)	312, 430, 750, 840, 871
MmeI	TCCRAC	1 cut(s)	787
Mph1103I	ATGCAT	1 cut(s)	951
MseI	TTAA	5 cut(s)	290, 396, 713, 747, 753
MslI	CAYNNNNRTG	1 cut(s)	642
MspA1I	CMGCKG	1 cut(s)	867
MunI	CAATTG	1 cut(s)	312
Mva1269I	GAATGC	2 cut(s)	20, 985
MwoI	GCNNNNNNNGC	6 cut(s)	92, 129, 359, 626, 931, 1020
NdeI	CATATG	1 cut(s)	278
NdeII	GATC	4 cut(s)	488, 523, 563, 885
NlaIII	CATG	4 cut(s)	173, 311, 389, 533
NlaIV	GGNNCC	1 cut(s)	490
NsiI	ATGCAT	1 cut(s)	951
PagI	TCATGA	1 cut(s)	169
PcsI	WCGNNNNNNNCGW	1 cut(s)	183
PctI	GAATGC	2 cut(s)	20, 985
PfeI	GAWTC	4 cut(s)	284, 402, 657, 1056
PkrI	GCNGC	4 cut(s)	237, 866, 869, 1022
Ppu21I	YACGTR	1 cut(s)	338
PpuMI	RGGWCCY	1 cut(s)	742
PsiI	TTATAA	1 cut(s)	675
Psp5II	RGGWCCY	1 cut(s)	742
PspN4I	GGNNCC	1 cut(s)	490
PspPI	GGNCC	2 cut(s)	299, 742
PspPPI	RGGWCCY	1 cut(s)	742
PsuI	RGATCY	1 cut(s)	488
RseI	CAYNNNNRTG	1 cut(s)	642
SaqAI	TTAA	5 cut(s)	290, 396, 713, 747, 753
SatI	GCNGC	4 cut(s)	236, 865, 868, 1021
Sau3AI	GATC	4 cut(s)	488, 523, 563, 885
Sau96I	GGNCC	2 cut(s)	299, 742
SduI	GDGCHC	2 cut(s)	8, 732
SfaNI	GCATC	4 cut(s)	340, 433, 936, 958
SinI	GGWCC	1 cut(s)	742
SmiMI	CAYNNNNRTG	1 cut(s)	642
SmlI	CTYRAG	1 cut(s)	786
SmoI	CTYRAG	1 cut(s)	786
Sse9I	AATT	5 cut(s)	312, 430, 750, 840, 871
SsiI	CCGC	4 cut(s)	30, 51, 130, 867
SspMI	CTAG	3 cut(s)	195, 669, 1065
StyI	CCWWGG	2 cut(s)	259, 1064
TaaI	ACNGT	4 cut(s)	81, 424, 545, 734
TaiI	ACGT	3 cut(s)	340, 702, 1051
TaqI	TCGA	1 cut(s)	1095
TasI	AATT	5 cut(s)	312, 430, 750, 840, 871
TauI	GCSGC	1 cut(s)	870
TfiI	GAWTC	4 cut(s)	284, 402, 657, 1056
Tru1I	TTAA	5 cut(s)	290, 396, 713, 747, 753
Tru9I	TTAA	5 cut(s)	290, 396, 713, 747, 753
TscAI	CASTG	4 cut(s)	205, 369, 737, 1021
TseI	GCWGC	3 cut(s)	235, 864, 1020
TspDTI	ATGAA	6 cut(s)	546, 626, 699, 777, 851, 974
TspRI	CASTG	4 cut(s)	205, 369, 737, 1021
VpaK11BI	GGWCC	1 cut(s)	742
XmaJI	CCTAGG	1 cut(s)	1064
XmiI	GTMKAC	1 cut(s)	780
XspI	CTAG	3 cut(s)	195, 669, 1065
Zsp2I	ATGCAT	1 cut(s)	951

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.