MD01G1072000.v1.1

Desiccation protectant protein Lea14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016919
Physical Location & Seq
Forward (+)
17729402 .. 17730170
769 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1072000.v1.1.491

Sequence Viewer

Length: 513 bp
ATGTGTTCCCAAGAAAGACGTAGTGCCAAGCAAGGTCAGGGGTATGTGGAATGGAGATTGGCTGAGATGAAGAAAAGATCTGCTCTTATTGTAGGCCTTCAATTTGGGAACCAAAGCTTGGACTCCACAGAACTCGTCTTCGAGATATCTATCTTCAACCCCTTTGAGTTCTATCTCCCTGTCAGTCAGGCTTCTTTCAAATTCAAAAGCGCCGGCAGCGAGGTCTTAACAGGAACATTGAAAGAGTCCGCGTCACTGAAGCCCGGAGAAGAGTCCATTATGTTTGTGTTGGTGAAGGTGCCGTTAAGAGTGTTCATAAACTTGGCAAAGGACATTTCTGGAGATGCGGACATTAACTATGAGATCGATTTGAGTATCAAGATTAAGCTTCCTATAATTGGGAACGTTGTATCCTTACCAATTCCTTGCGAATGCCAACAGGGCGAACTCAAGCTTTCTCATCTGTTTTCCAAATTGTTGGACCAAATCAATCGTCAGCAGCTGGTGCCTTAA

Protein Analysis

171

Amino Acids

19.01

Weight (kDa)

6.6

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 49 - 145 9e-09 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016919)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g15470
malus_domestica MD01G1072000.v1.1
prunus_persica Prupe.2G178300_v2.0.a1
pyrus_communis pycom01g10120
rosa_chinensis RchiOBHm_Chr1g0357781
rosa_laevigata RLG00000027997
rosa_multiflora Rmu_sc0011109.1_g000014
rosa_roxburghii Rroxscaffold_4G00298520
rosa_rugosa Rorug01G0258600
rosa_samantha Rh1AG272500 Rh1BG239700 Rh1CG256400 Rh1DG268000
rosa_wichuraiana Rw1G024320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 298, 505
AccB7I CCANNNNNTGG 1 cut(s) 118
AccII CGCG 1 cut(s) 251
AciI CCGC 2 cut(s) 249, 347
AclI AACGTT 1 cut(s) 405
AcsI RAATTY 1 cut(s) 200
AcuI CTGAAG 1 cut(s) 278
AfiI CCNNNNNNNGG 2 cut(s) 118, 398
AgsI TTSAA 5 cut(s) 101, 157, 199, 205, 241
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AloI GAACNNNNNNTCC 2 cut(s) 395, 427
AluBI AGCT 4 cut(s) 117, 388, 454, 502
AluI AGCT 4 cut(s) 117, 388, 454, 502
AlwNI CAGNNNCTG 1 cut(s) 502
AoxI GGCC 1 cut(s) 94
ApeKI GCWGC 2 cut(s) 216, 499
ApoI RAATTY 1 cut(s) 200
ArsI GACNNNNNNTTYG 2 cut(s) 478, 510
AspLEI GCGC 1 cut(s) 212
AspS9I GGNCC 1 cut(s) 481
AsuC2I CCSGG 1 cut(s) 264
AsuHPI GGTGA 1 cut(s) 304
AvaII GGWCC 1 cut(s) 481
BanI GGYRCC 2 cut(s) 298, 505
BbsI GAAGAC 1 cut(s) 130
BbvI GCAGC 1 cut(s) 228
BceAI ACGGC 1 cut(s) 286
BciVI GTATCC 1 cut(s) 421
BcnI CCSGG 1 cut(s) 264
BfoI RGCGCY 1 cut(s) 213
BfuI GTATCC 1 cut(s) 421
BglI GCCNNNNNGGC 1 cut(s) 441
BglII AGATCT 1 cut(s) 77
BisI GCNGC 2 cut(s) 217, 500
BlsI GCNGC 2 cut(s) 218, 501
Bme1390I CCNGG 1 cut(s) 264
Bme18I GGWCC 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 481
BmiI GGNNCC 3 cut(s) 110, 300, 507
BmrFI CCNGG 1 cut(s) 264
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 1 cut(s) 130
BpmI CTGGAG 1 cut(s) 360
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 264
Bsa29I ATCGAT 1 cut(s) 366
BsaBI GATNNNNATC 1 cut(s) 149
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 398
Bse118I RCCGGY 1 cut(s) 212
Bse8I GATNNNNATC 1 cut(s) 149
BseCI ATCGAT 1 cut(s) 366
BseJI GATNNNNATC 1 cut(s) 149
BseLI CCNNNNNNNGG 2 cut(s) 118, 398
BseMII CTCAG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 228
Bsh1236I CGCG 1 cut(s) 251
BshFI GGCC 1 cut(s) 96
BshNI GGYRCC 2 cut(s) 298, 505
BshVI ATCGAT 1 cut(s) 366
BsiSI CCGG 2 cut(s) 213, 264
BslI CCNNNNNNNGG 2 cut(s) 118, 398
BsmI GAATGC 1 cut(s) 437
BsnI GGCC 1 cut(s) 96
Bsp143I GATC 2 cut(s) 77, 363
BspACI CCGC 2 cut(s) 249, 347
BspANI GGCC 1 cut(s) 96
BspCNI CTCAG 1 cut(s) 55
BspDI ATCGAT 1 cut(s) 366
BspFNI CGCG 1 cut(s) 251
BspLI GGNNCC 3 cut(s) 110, 300, 507
BspT107I GGYRCC 2 cut(s) 298, 505
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssMI GATC 2 cut(s) 77, 363
Bst6I CTCTTC 1 cut(s) 264
BstAPI GCANNNNNTGC 1 cut(s) 505
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 251
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 2 cut(s) 80, 366
BstMBI GATC 2 cut(s) 77, 363
BstMWI GCNNNNNNNGC 3 cut(s) 216, 441, 505
BstSCI CCNGG 1 cut(s) 262
BstUI CGCG 1 cut(s) 251
BstV1I GCAGC 1 cut(s) 228
BstV2I GAAGAC 1 cut(s) 130
BstX2I RGATCY 1 cut(s) 77
BstXI CCANNNNNNTGG 1 cut(s) 478
BstYI RGATCY 1 cut(s) 77
Bsu15I ATCGAT 1 cut(s) 366
BsuI GTATCC 1 cut(s) 421
BsuRI GGCC 1 cut(s) 96
BsuTUI ATCGAT 1 cut(s) 366
BtsIMutI CAGTG 1 cut(s) 254
Cac8I GCNNGC 1 cut(s) 214
CaiI CAGNNNCTG 1 cut(s) 502
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 1 cut(s) 481
ClaI ATCGAT 1 cut(s) 366
CseI GACGC 1 cut(s) 240
CviJI RGCY 8 cut(s) 62, 96, 117, 191, 262, 388, 454, 502
CviKI_1 RGCY 8 cut(s) 62, 96, 117, 191, 262, 388, 454, 502
DdeI CTNAG 1 cut(s) 63
DpnI GATC 2 cut(s) 79, 365
DpnII GATC 2 cut(s) 77, 363
Eam1104I CTCTTC 1 cut(s) 264
EarI CTCTTC 1 cut(s) 264
Eco147I AGGCCT 1 cut(s) 96
Eco32I GATATC 1 cut(s) 147
Eco47I GGWCC 1 cut(s) 481
Eco57I CTGAAG 1 cut(s) 278
EcoRV GATATC 1 cut(s) 147
FaiI YATR 5 cut(s) 45, 281, 317, 360, 395
Fnu4HI GCNGC 2 cut(s) 217, 500
Fsp4HI GCNGC 2 cut(s) 217, 500
GlaI GCGC 1 cut(s) 211
GluI GCNGC 2 cut(s) 217, 500
GsuI CTGGAG 1 cut(s) 360
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 96
HapII CCGG 2 cut(s) 213, 264
HgaI GACGC 1 cut(s) 240
HhaI GCGC 1 cut(s) 212
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 3 cut(s) 115, 386, 452
HinfI GANTC 3 cut(s) 122, 245, 272
HpaII CCGG 2 cut(s) 213, 264
HphI GGTGA 1 cut(s) 304
Hpy188III TCNNGA 3 cut(s) 142, 339, 379
HpyAV CCTTC 2 cut(s) 107, 289
HpyCH4IV ACGT 2 cut(s) 19, 405
HpyF10VI GCNNNNNNNGC 3 cut(s) 216, 441, 505
HpyF3I CTNAG 1 cut(s) 63
HpySE526I ACGT 2 cut(s) 19, 405
HspAI GCGC 1 cut(s) 210
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Kzo9I GATC 2 cut(s) 77, 363
LpnPI CCDG 9 cut(s) 23, 173, 192, 216, 226, 277, 324, 425, 488
Lsp1109I GCAGC 1 cut(s) 228
LweI GCATC 1 cut(s) 334
MaeII ACGT 2 cut(s) 19, 405
MaeIII GTNAC 1 cut(s) 252
MalI GATC 2 cut(s) 79, 365
MboI GATC 2 cut(s) 77, 363
MboII GAAGA 4 cut(s) 82, 130, 145, 281
MflI RGATCY 1 cut(s) 77
MluCI AATT 5 cut(s) 101, 200, 396, 420, 473
MlyI GAGTC 3 cut(s) 116, 254, 281
MmeI TCCRAC 1 cut(s) 459
MnlI CCTC 1 cut(s) 214
MroNI GCCGGC 1 cut(s) 212
MseI TTAA 5 cut(s) 227, 305, 354, 384, 511
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 2 cut(s) 213, 264
MspR9I CCNGG 1 cut(s) 264
Mva1269I GAATGC 1 cut(s) 437
MvnI CGCG 1 cut(s) 251
MwoI GCNNNNNNNGC 3 cut(s) 216, 441, 505
NaeI GCCGGC 1 cut(s) 214
NciI CCSGG 1 cut(s) 264
NdeII GATC 2 cut(s) 77, 363
NgoMIV GCCGGC 1 cut(s) 212
NlaIV GGNNCC 3 cut(s) 110, 300, 507
NmuCI GTSAC 1 cut(s) 252
PceI AGGCCT 1 cut(s) 96
PctI GAATGC 1 cut(s) 437
PdiI GCCGGC 1 cut(s) 214
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 2 cut(s) 218, 501
PleI GAGTC 3 cut(s) 116, 253, 280
PpsI GAGTC 3 cut(s) 116, 253, 280
Psp1406I AACGTT 1 cut(s) 405
PspN4I GGNNCC 3 cut(s) 110, 300, 507
PspPI GGNCC 1 cut(s) 481
PstNI CAGNNNCTG 1 cut(s) 502
PsuI RGATCY 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 502
SaqAI TTAA 5 cut(s) 227, 305, 354, 384, 511
SatI GCNGC 2 cut(s) 217, 500
Sau3AI GATC 2 cut(s) 77, 363
Sau96I GGNCC 1 cut(s) 481
SchI GAGTC 3 cut(s) 116, 254, 281
ScrFI CCNGG 1 cut(s) 264
SetI ASST 9 cut(s) 22, 37, 119, 225, 300, 390, 408, 456, 504
SfaNI GCATC 1 cut(s) 334
SinI GGWCC 1 cut(s) 481
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 5 cut(s) 101, 200, 396, 420, 473
SseBI AGGCCT 1 cut(s) 96
SsiI CCGC 2 cut(s) 249, 347
StuI AGGCCT 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 262
TaiI ACGT 2 cut(s) 22, 408
TaqI TCGA 2 cut(s) 141, 366
TasI AATT 5 cut(s) 101, 200, 396, 420, 473
Tru1I TTAA 5 cut(s) 227, 305, 354, 384, 511
Tru9I TTAA 5 cut(s) 227, 305, 354, 384, 511
TscAI CASTG 1 cut(s) 261
TseFI GTSAC 1 cut(s) 252
TseI GCWGC 2 cut(s) 216, 499
Tsp45I GTSAC 1 cut(s) 252
TspDTI ATGAA 2 cut(s) 83, 304
TspRI CASTG 1 cut(s) 261
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 481
XapI RAATTY 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.