RLG00000027997

Desiccation protectant protein Lea14 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016919
Physical Location & Seq
Reverse (-)
17579726 .. 17580837
1112 bp
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UTR
Exon/CDS
Intron
RLM00000027997

Sequence Viewer

Length: 561 bp
ATGTCTCTGTTTTTGTCTTCAACAGCGAACATTTTAAGCTCTCTGTATTTGAAAGTGTCGGGTTTATTGGGCAATGCCAAGAACTATATTGTGAACAAGGCCAGGGGTTATGTTTTGGCAAGGTTGGTTGAGATGCAGAGAAGGTCTGCGCTTATTGTAGACCTTCAACTTGAGGATATAAGCTTCGACTCCATATATCTCCTTTTCAAGATCTCTGTGTACAACTCATTTCAGTTTTCTCTGCCTGTCAGTCAAGCCTCCTTCAAAATGAAAATCGGTGGCAGTGAACCTTTATTAGGGAAATTGAAGGAGCCTGTGCACATAAAGGCAGGAGCAGATTCTATATTGCTTGTGTCAGCGAAGGTGCCATTTAGTATGCCTATAAATTTGGCAAATGACATTTTTGTAGATGCAGACATTGACTACCGCATCGATTTCCAACTCACAATTCACCTTCCTATCATCGGCAACATCTCTTTACCACTTCCTTGCCAACAAGGCGAGACCAAGATCTCCGATTTGTTTAGCAATTTGATGGATCAAATCATCATGAGTTGGTGA

Protein Analysis

187

Amino Acids

20.67

Weight (kDa)

6.72

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 70 - 165 7.6e-10 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016919)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g15470
malus_domestica MD01G1072000.v1.1
prunus_persica Prupe.2G178300_v2.0.a1
pyrus_communis pycom01g10120
rosa_chinensis RchiOBHm_Chr1g0357781
rosa_laevigata RLG00000027997
rosa_multiflora Rmu_sc0011109.1_g000014
rosa_roxburghii Rroxscaffold_4G00298520
rosa_rugosa Rorug01G0258600
rosa_samantha Rh1AG272500 Rh1BG239700 Rh1CG256400 Rh1DG268000
rosa_wichuraiana Rw1G024320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 364
AccI GTMKAC 1 cut(s) 159
AciI CCGC 1 cut(s) 427
AclWI GGATC 1 cut(s) 546
AcsI RAATTY 1 cut(s) 385
AfaI GTAC 1 cut(s) 221
AfiI CCNNNNNNNGG 2 cut(s) 296, 464
AgsI TTSAA 6 cut(s) 21, 52, 167, 208, 265, 307
AjnI CCWGG 1 cut(s) 101
AjuI GAANNNNNNNTTGG 2 cut(s) 432, 464
AluBI AGCT 2 cut(s) 39, 183
AluI AGCT 2 cut(s) 39, 183
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 2 cut(s) 9, 497
Alw44I GTGCAC 1 cut(s) 317
AlwI GGATC 1 cut(s) 546
AoxI GGCC 1 cut(s) 99
ApaLI GTGCAC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 385
AspLEI GCGC 1 cut(s) 151
AsuHPI GGTGA 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 321
BanI GGYRCC 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 9
Bbv12I GWGCWC 1 cut(s) 321
BccI CCATC 1 cut(s) 529
BciT130I CCWGG 1 cut(s) 103
BcoDI GTCTC 2 cut(s) 9, 497
BglI GCCNNNNNGGC 1 cut(s) 498
BglII AGATCT 2 cut(s) 210, 510
Bme1390I CCNGG 1 cut(s) 103
BmiI GGNNCC 2 cut(s) 312, 366
BmrFI CCNGG 1 cut(s) 103
BmsI GCATC 3 cut(s) 123, 400, 438
BpiI GAAGAC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 191
Bsa29I ATCGAT 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 497
BsaJI CCNNGG 1 cut(s) 102
Bsc4I CCNNNNNNNGG 2 cut(s) 296, 464
Bse3DI GCAATG 1 cut(s) 79
BseBI CCWGG 1 cut(s) 103
BseCI ATCGAT 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 102
BseLI CCNNNNNNNGG 2 cut(s) 296, 464
BseMI GCAATG 1 cut(s) 79
BseSI GKGCMC 1 cut(s) 321
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 364
BshVI ATCGAT 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 321
BslI CCNNNNNNNGG 2 cut(s) 296, 464
BsmAI GTCTC 2 cut(s) 9, 497
BsnI GGCC 1 cut(s) 101
Bso31I GGTCTC 1 cut(s) 497
Bsp1286I GDGCHC 1 cut(s) 321
Bsp1407I TGTACA 1 cut(s) 219
Bsp143I GATC 3 cut(s) 210, 510, 538
BspACI CCGC 1 cut(s) 427
BspANI GGCC 1 cut(s) 101
BspDI ATCGAT 1 cut(s) 432
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 2 cut(s) 312, 366
BspPI GGATC 1 cut(s) 546
BspT107I GGYRCC 1 cut(s) 364
BspTNI GGTCTC 1 cut(s) 497
BsrDI GCAATG 1 cut(s) 79
BsrGI TGTACA 1 cut(s) 219
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 3 cut(s) 210, 510, 538
Bst2UI CCWGG 1 cut(s) 103
BstAUI TGTACA 1 cut(s) 219
BstENI CCTNNNNNAGG 1 cut(s) 294
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 3 cut(s) 213, 513, 541
BstMAI GTCTC 2 cut(s) 9, 497
BstMBI GATC 3 cut(s) 210, 510, 538
BstMWI GCNNNNNNNGC 1 cut(s) 498
BstNI CCWGG 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 101
BstSLI GKGCMC 1 cut(s) 321
BstV2I GAAGAC 1 cut(s) 9
BstX2I RGATCY 2 cut(s) 210, 510
BstYI RGATCY 2 cut(s) 210, 510
Bsu15I ATCGAT 1 cut(s) 432
BsuRI GGCC 1 cut(s) 101
BsuTUI ATCGAT 1 cut(s) 432
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 1 cut(s) 289
CciI TCATGA 1 cut(s) 549
CfoI GCGC 1 cut(s) 151
ClaI ATCGAT 1 cut(s) 432
Csp6I GTAC 1 cut(s) 220
CviAII CATG 1 cut(s) 550
CviJI RGCY 5 cut(s) 39, 101, 183, 257, 313
CviKI_1 RGCY 5 cut(s) 39, 101, 183, 257, 313
CviQI GTAC 1 cut(s) 220
DpnI GATC 3 cut(s) 212, 512, 540
DpnII GATC 3 cut(s) 210, 510, 538
Eco31I GGTCTC 1 cut(s) 497
EcoNI CCTNNNNNAGG 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 101
FaeI CATG 1 cut(s) 553
FatI CATG 1 cut(s) 549
FblI GTMKAC 1 cut(s) 159
GlaI GCGC 1 cut(s) 150
HaeIII GGCC 1 cut(s) 101
HhaI GCGC 1 cut(s) 151
Hin1II CATG 1 cut(s) 553
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HindIII AAGCTT 1 cut(s) 181
HinfI GANTC 2 cut(s) 188, 338
HphI GGTGA 1 cut(s) 443
Hpy166II GTNNAC 5 cut(s) 94, 160, 220, 287, 319
Hpy188I TCNGA 1 cut(s) 517
Hpy188III TCNNGA 2 cut(s) 208, 550
Hpy8I GTNNAC 5 cut(s) 94, 160, 220, 287, 319
HpyAV CCTTC 6 cut(s) 135, 173, 271, 301, 355, 464
HpyCH4V TGCA 3 cut(s) 136, 319, 413
HpyF10VI GCNNNNNNNGC 1 cut(s) 498
Hsp92II CATG 1 cut(s) 553
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 3 cut(s) 210, 510, 538
LmnI GCTCC 2 cut(s) 310, 332
LpnPI CCDG 5 cut(s) 88, 115, 258, 315, 327
LweI GCATC 3 cut(s) 123, 400, 438
MalI GATC 3 cut(s) 212, 512, 540
MboI GATC 3 cut(s) 210, 510, 538
MboII GAAGA 1 cut(s) 9
MflI RGATCY 2 cut(s) 210, 510
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 4 cut(s) 302, 385, 447, 529
MlyI GAGTC 1 cut(s) 182
MmeI TCCRAC 1 cut(s) 463
MnlI CCTC 2 cut(s) 166, 268
MseI TTAA 1 cut(s) 35
MspR9I CCNGG 1 cut(s) 103
MvaI CCWGG 1 cut(s) 103
MwoI GCNNNNNNNGC 1 cut(s) 498
NdeII GATC 3 cut(s) 210, 510, 538
NlaIII CATG 1 cut(s) 553
NlaIV GGNNCC 2 cut(s) 312, 366
PagI TCATGA 1 cut(s) 549
PfeI GAWTC 1 cut(s) 338
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 101
PspGI CCWGG 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 312, 366
PsuI RGATCY 2 cut(s) 210, 510
RsaI GTAC 1 cut(s) 221
RsaNI GTAC 1 cut(s) 220
SaqAI TTAA 1 cut(s) 35
Sau3AI GATC 3 cut(s) 210, 510, 538
SchI GAGTC 1 cut(s) 182
ScrFI CCNGG 1 cut(s) 103
SduI GDGCHC 1 cut(s) 321
SetI ASST 8 cut(s) 41, 125, 146, 165, 185, 292, 366, 456
SfaNI GCATC 3 cut(s) 123, 400, 438
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 4 cut(s) 302, 385, 447, 529
SsiI CCGC 1 cut(s) 427
StyD4I CCNGG 1 cut(s) 101
TaqI TCGA 2 cut(s) 186, 432
TasI AATT 4 cut(s) 302, 385, 447, 529
TatI WGTACW 1 cut(s) 219
TfiI GAWTC 1 cut(s) 338
Tru1I TTAA 1 cut(s) 35
Tru9I TTAA 1 cut(s) 35
TscAI CASTG 1 cut(s) 289
TspDTI ATGAA 1 cut(s) 284
TspRI CASTG 1 cut(s) 289
VneI GTGCAC 1 cut(s) 317
XagI CCTNNNNNAGG 1 cut(s) 294
XapI RAATTY 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.