MD01G1072100.v1.1

Late embryogenesis abundant protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014279
Physical Location & Seq
Forward (+)
17753444 .. 17754106
663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1072100.v1.1.491

Sequence Viewer

Length: 663 bp
ATGGTGGACGGGGAGCAAGTCAGACCGCTAGCCCCTGCCGCTATCCACCCAAGCAGCGACGCCGATGAGGCTGCTTTTCACTTGAAAAAAGTTAGACGAAGAAAGTTCATCAAGTGCTGCGGCTGCATAACAGCTGTGATCTTAATCCAAGCCGTTGTGATCATAATATTAGCTTTCACTGTGTTCCGTGTGAAGGAGCCAAAGATCAAGATGAATAAGATCACGATTACAAGGCTGGAGTTGATCAACAACAACACAGCCCCTAAGCCAGGCTCAAACCTGTCACTAATAGCCGATGTGTCGGTGAAAAATCCCAACTTCGCCTCGTTCAAGTATACCAACACCACCACGACCTTGTATTATCACGGCGTGGTGGTGGGGGAGGCTCATGGCGCACCTGGTCATGCTAAGGCTCGACGGACCATGAGGATGAATATCACGGTTGATATGATCACAGACCGGCTCACGTCCGACCCCAACTTGAGGGCGGATTTCAATTCTGGGTTGATGACCATGAGTAGCTATTCTAGAATTCCAGGAAGGGTGAAGATGTTGAATATTATCAAGAAACATGTGATTGTGAAAATGAATTGTACTATGACTGTTAATATTTCCAGCCAGGCAATTCAAGAACAAAAATGTAAGAGGAAGGTTAAGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

221

Amino Acids

24.49

Weight (kDa)

10.16

Isoelectric Point (pI)

36.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 100 - 199 5.7e-12 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014279)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46150
fragaria_vesca FvH4_7g15490
malus_domestica MD01G1072100.v1.1 MD07G1130600.v1.1
prunus_persica Prupe.2G178500_v2.0.a1
pyrus_communis pycom01g10140
rosa_chinensis RchiOBHm_Chr1g0357811
rosa_laevigata RLG00000027994
rosa_multiflora Rmu_co8384079.1_g000001 Rmu_sc0011109.1_g000008
rosa_roxburghii Rroxscaffold_4G00298480
rosa_rugosa Rorug01G0258800
rosa_samantha Rh1AG272700 Rh1BG240000 Rh1CG256600 Rh1DG268300
rosa_wichuraiana Rw1G024340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 335
AciI CCGC 4 cut(s) 26, 39, 120, 488
AcsI RAATTY 1 cut(s) 531
AcyI GRCGYC 1 cut(s) 60
AdeI CACNNNGTG 1 cut(s) 370
AfaI GTAC 1 cut(s) 595
AfiI CCNNNNNNNGG 3 cut(s) 193, 269, 483
AflIII ACRYGT 1 cut(s) 571
AgsI TTSAA 5 cut(s) 85, 331, 496, 556, 629
AjiI CACGTC 1 cut(s) 468
AjnI CCWGG 4 cut(s) 268, 397, 535, 618
AluBI AGCT 4 cut(s) 134, 173, 522, 658
AluI AGCT 4 cut(s) 134, 173, 522, 658
ApeKI GCWGC 4 cut(s) 54, 71, 117, 123
ApoI RAATTY 1 cut(s) 531
AspLEI GCGC 1 cut(s) 395
AspS9I GGNCC 1 cut(s) 420
AsuHPI GGTGA 2 cut(s) 316, 556
AsuNHI GCTAGC 1 cut(s) 28
AvaII GGWCC 1 cut(s) 420
BbvI GCAGC 4 cut(s) 58, 66, 104, 110
BceAI ACGGC 2 cut(s) 137, 382
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BciT130I CCWGG 4 cut(s) 270, 399, 537, 620
BclI TGATCA 3 cut(s) 159, 243, 450
BfaI CTAG 3 cut(s) 29, 528, 661
BglI GCCNNNNNGGC 1 cut(s) 68
BisI GCNGC 6 cut(s) 39, 55, 72, 118, 121, 124
BlsI GCNGC 6 cut(s) 40, 56, 73, 119, 122, 125
Bme1390I CCNGG 4 cut(s) 270, 399, 537, 620
Bme18I GGWCC 1 cut(s) 420
BmgBI CACGTC 1 cut(s) 468
BmgT120I GGNCC 1 cut(s) 420
BmiI GGNNCC 1 cut(s) 198
BmrFI CCNGG 4 cut(s) 270, 399, 537, 620
BmtI GCTAGC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 257
Bpu10I CCTNAGC 2 cut(s) 264, 408
BpuEI CTTGAG 1 cut(s) 502
BsaBI GATNNNNATC 2 cut(s) 143, 434
BsaHI GRCGYC 1 cut(s) 60
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 269, 483
Bse118I RCCGGY 1 cut(s) 459
Bse8I GATNNNNATC 2 cut(s) 143, 434
BseBI CCWGG 4 cut(s) 270, 399, 537, 620
BseGI GGATG 1 cut(s) 435
BseJI GATNNNNATC 2 cut(s) 143, 434
BseLI CCNNNNNNNGG 3 cut(s) 193, 269, 483
BseXI GCAGC 4 cut(s) 58, 66, 104, 110
BsiSI CCGG 1 cut(s) 460
BslI CCNNNNNNNGG 3 cut(s) 193, 269, 483
Bsp143I GATC 6 cut(s) 138, 159, 204, 219, 243, 450
BspACI CCGC 4 cut(s) 26, 39, 120, 488
BspLI GGNNCC 1 cut(s) 198
BspOI GCTAGC 1 cut(s) 32
BsrFI RCCGGY 1 cut(s) 459
BssAI RCCGGY 1 cut(s) 459
BssMI GATC 6 cut(s) 138, 159, 204, 219, 243, 450
BssNAI GTATAC 1 cut(s) 336
BssNI GRCGYC 1 cut(s) 60
Bst1107I GTATAC 1 cut(s) 336
Bst2UI CCWGG 4 cut(s) 270, 399, 537, 620
Bst4CI ACNGT 3 cut(s) 181, 442, 604
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 30
BstDEI CTNAG 2 cut(s) 264, 408
BstENI CCTNNNNNAGG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 435
BstHHI GCGC 1 cut(s) 395
BstKTI GATC 6 cut(s) 141, 162, 207, 222, 246, 453
BstMBI GATC 6 cut(s) 138, 159, 204, 219, 243, 450
BstMWI GCNNNNNNNGC 4 cut(s) 38, 68, 123, 392
BstNI CCWGG 4 cut(s) 270, 399, 537, 620
BstNSI RCATGY 1 cut(s) 575
BstSCI CCNGG 4 cut(s) 268, 397, 535, 618
BstV1I GCAGC 4 cut(s) 58, 66, 104, 110
BstZ17I GTATAC 1 cut(s) 336
BtrI CACGTC 1 cut(s) 468
BtsCI GGATG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 30
CfoI GCGC 1 cut(s) 395
Cfr10I RCCGGY 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 420
CseI GACGC 1 cut(s) 68
CsiI ACCWGGT 1 cut(s) 397
Csp6I GTAC 1 cut(s) 594
CviAII CATG 5 cut(s) 389, 404, 424, 514, 572
CviQI GTAC 1 cut(s) 594
DdeI CTNAG 2 cut(s) 264, 408
DpnI GATC 6 cut(s) 140, 161, 206, 221, 245, 452
DpnII GATC 6 cut(s) 138, 159, 204, 219, 243, 450
DraIII CACNNNGTG 1 cut(s) 370
EciI GGCGGA 1 cut(s) 503
Eco47I GGWCC 1 cut(s) 420
EcoNI CCTNNNNNAGG 1 cut(s) 267
EcoRI GAATTC 1 cut(s) 531
EcoRII CCWGG 4 cut(s) 268, 397, 535, 618
FaeI CATG 5 cut(s) 392, 407, 427, 517, 575
FatI CATG 5 cut(s) 388, 403, 423, 513, 571
FbaI TGATCA 3 cut(s) 159, 243, 450
FblI GTMKAC 1 cut(s) 335
Fnu4HI GCNGC 6 cut(s) 39, 55, 72, 118, 121, 124
FokI GGATG 1 cut(s) 442
Fsp4HI GCNGC 6 cut(s) 39, 55, 72, 118, 121, 124
FspBI CTAG 3 cut(s) 29, 528, 661
GlaI GCGC 1 cut(s) 394
GluI GCNGC 6 cut(s) 39, 55, 72, 118, 121, 124
GsuI CTGGAG 1 cut(s) 257
HapII CCGG 1 cut(s) 460
HgaI GACGC 1 cut(s) 68
HhaI GCGC 1 cut(s) 395
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 5 cut(s) 392, 407, 427, 517, 575
Hin6I GCGC 1 cut(s) 393
HinP1I GCGC 1 cut(s) 393
HpaII CCGG 1 cut(s) 460
HphI GGTGA 2 cut(s) 316, 556
Hpy166II GTNNAC 2 cut(s) 7, 336
Hpy188I TCNGA 2 cut(s) 23, 472
Hpy188III TCNNGA 5 cut(s) 208, 223, 528, 565, 629
Hpy8I GTNNAC 2 cut(s) 7, 336
Hpy99I CGWCG 2 cut(s) 62, 420
HpyAV CCTTC 3 cut(s) 187, 534, 643
HpyCH4III ACNGT 3 cut(s) 181, 442, 604
HpyCH4IV ACGT 1 cut(s) 467
HpyCH4V TGCA 1 cut(s) 126
HpyF10VI GCNNNNNNNGC 4 cut(s) 38, 68, 123, 392
HpyF3I CTNAG 2 cut(s) 264, 408
HpySE526I ACGT 1 cut(s) 467
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 5 cut(s) 392, 407, 427, 517, 575
HspAI GCGC 1 cut(s) 393
Ksp22I TGATCA 3 cut(s) 159, 243, 450
Kzo9I GATC 6 cut(s) 138, 159, 204, 219, 243, 450
LmnI GCTCC 2 cut(s) 13, 196
Lsp1109I GCAGC 4 cut(s) 58, 66, 104, 110
MabI ACCWGGT 1 cut(s) 397
MaeI CTAG 3 cut(s) 29, 528, 661
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 1 cut(s) 282
MalI GATC 6 cut(s) 140, 161, 206, 221, 245, 452
MboI GATC 6 cut(s) 138, 159, 204, 219, 243, 450
MboII GAAGA 2 cut(s) 111, 559
MluCI AATT 4 cut(s) 496, 531, 589, 624
MmeI TCCRAC 1 cut(s) 495
MnlI CCTC 6 cut(s) 61, 334, 376, 420, 477, 639
MseI TTAA 3 cut(s) 143, 606, 654
MslI CAYNNNNRTG 1 cut(s) 428
MspA1I CMGCKG 1 cut(s) 134
MspI CCGG 1 cut(s) 460
MspR9I CCNGG 4 cut(s) 270, 399, 537, 620
MvaI CCWGG 4 cut(s) 270, 399, 537, 620
MwoI GCNNNNNNNGC 4 cut(s) 38, 68, 123, 392
NdeII GATC 6 cut(s) 138, 159, 204, 219, 243, 450
NheI GCTAGC 1 cut(s) 28
NlaIII CATG 5 cut(s) 392, 407, 427, 517, 575
NlaIV GGNNCC 1 cut(s) 198
NmuCI GTSAC 1 cut(s) 282
NspI RCATGY 1 cut(s) 575
PciI ACATGT 1 cut(s) 571
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 6 cut(s) 40, 56, 73, 119, 122, 125
PscI ACATGT 1 cut(s) 571
Psp6I CCWGG 4 cut(s) 268, 397, 535, 618
PspGI CCWGG 4 cut(s) 268, 397, 535, 618
PspN4I GGNNCC 1 cut(s) 198
PspPI GGNCC 1 cut(s) 420
PvuII CAGCTG 1 cut(s) 134
RsaI GTAC 1 cut(s) 595
RsaNI GTAC 1 cut(s) 594
RseI CAYNNNNRTG 1 cut(s) 428
SaqAI TTAA 3 cut(s) 143, 606, 654
SatI GCNGC 6 cut(s) 39, 55, 72, 118, 121, 124
Sau3AI GATC 6 cut(s) 138, 159, 204, 219, 243, 450
Sau96I GGNCC 1 cut(s) 420
ScrFI CCNGG 4 cut(s) 270, 399, 537, 620
SetI ASST 9 cut(s) 136, 175, 282, 356, 400, 470, 524, 654, 660
SexAI ACCWGGT 1 cut(s) 397
SinI GGWCC 1 cut(s) 420
SmiMI CAYNNNNRTG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 4 cut(s) 496, 531, 589, 624
SsiI CCGC 4 cut(s) 26, 39, 120, 488
SspI AATATT 3 cut(s) 168, 559, 610
SspMI CTAG 3 cut(s) 29, 528, 661
StyD4I CCNGG 4 cut(s) 268, 397, 535, 618
TaaI ACNGT 3 cut(s) 181, 442, 604
TaiI ACGT 1 cut(s) 470
TaqI TCGA 1 cut(s) 415
TasI AATT 4 cut(s) 496, 531, 589, 624
TatI WGTACW 1 cut(s) 593
TauI GCSGC 2 cut(s) 41, 123
Tru1I TTAA 3 cut(s) 143, 606, 654
Tru9I TTAA 3 cut(s) 143, 606, 654
TscAI CASTG 1 cut(s) 184
TseFI GTSAC 1 cut(s) 282
TseI GCWGC 4 cut(s) 54, 71, 117, 123
Tsp45I GTSAC 1 cut(s) 282
TspDTI ATGAA 4 cut(s) 97, 227, 446, 602
TspGWI ACGGA 2 cut(s) 176, 433
TspRI CASTG 1 cut(s) 184
VpaK11BI GGWCC 1 cut(s) 420
XagI CCTNNNNNAGG 1 cut(s) 267
XapI RAATTY 1 cut(s) 531
XbaI TCTAGA 1 cut(s) 527
XceI RCATGY 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 335
XspI CTAG 3 cut(s) 29, 528, 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.