Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004117

Physical Location & Seq

Genomic Coordinates Forward (+)

18087223 .. 18092269

Gene Structure 5047 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1074700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1272 bp

ATGGGAAACGATGATGATGCAAAGTCTGTTAAATCTGAAAAATCATATTCGCCTCTGCCGGCAGATCAGGCGAATCCTAACAATCAGACCAATCTGCATGTCTATCCTGATTGGGCAGCCATGCAGGCTTATTATGGTCCGAGAGTTGCTCTCCCTCCATATTACAACTCAGCTATGGCTTCTGGCCACCCTCCTCATCCCTATATGTGGCCCCCACCACAGCATATGATGCCACCCTATGGGGCGCCTTATGCAGTATACTCGCATGGAGGTGTTTATGCACATCCGGCAGTTCCTCTTGCATCCCATGGTCAAGGGGGTCCATCCTTACCTGCTGCTGTGACTCCTTTGAATATGGAAACACCAACAAAGTCATCTGGTAATACAGACTGTGGGTTAATGAAAAAGTTGAAAGGATTTGATGGGCTTGCAATGTCAATAGGCAGTGGTAATGTTATGAATGCCGTAGGTGGAGCTGAGCGTAGTCTCTCCCAGAGTTCTGGGACTGAAGGTTCTAGCAATGGAAGTGATGGGAATACTGCTGCGGCAAATCAAACCAGAAGAAAAAGAAGTCGTGAGGGAACACCAGCCACTGCTGGAGAAGGGAAGACTGACATGCAGCCTGGACCAGCTGAGGAGGCAAGTGCAGCTACTGATAAAGTGTTGGGTGTAACTCTTGCTGCTGTTAGTATCCCGGGAAAATTGGTTGGACCAGCTGTTTCTCCTGGCATGACCACAGCGTTGGAACTCAAGAACTCGCCTATCTTGAACTCGAAGACAAGTCCTACCAGTGTTCCGCAATCCTGTGCAGTTTTGCCTCCGGAAACCTGGATACATAATGACCGGGAGATAAAACGGGAAAGGAGGAAGCAATCTAATCGGGAATCTGCTAGAAGATCCAGGTTGAGGAAGCAGGCTGAGACTGAAGAACTTGCACGAAAGGTCGAGGCCTTATCTGCAGATAATGTGGCACTGAAATCTGAAATAAACCGGTTAACCGAGAATTCTGCTACACTGAGGCTAGAAAACACTACATTACTGGATAAACTCAAAAACGCACGCGTAGGAAGAATGGAAGACATCATCATGAACATTGATGACAAGAGGGTCCAACCTTTCAGTACCGAGAACCTACTGTCAAGAGTTAACAACGCAGGTTCTATTGCGAGGGATGCTGAGAAAGACAGTGACACTTACGAGAAAAACAATGGGACGAAGCTGCACCAACTCCTGGATGCGAGTCCAAGGGCCGATGCAGTCGTTGCTGGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006082 GO:0006355 GO:0006790 GO:0006807 GO:0008150 GO:0008152 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009719 GO:0009725 GO:0009737 GO:0009889 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0016143 GO:0019219 GO:0019222 GO:0019748 GO:0019757 GO:0019760 GO:0031323 GO:0031326 GO:0033993 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0071704 GO:0080090 GO:0097159 GO:0097305 GO:0140110 GO:1901135 GO:1901363 GO:1901564 GO:1901657 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFMR MFMR_assoc bZIP_1

Protein Analysis

424

Amino Acids

45.14

Weight (kDa)

8.62

Isoelectric Point (pI)

47.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MFMR	PF07777	1 - 99	1.1e-31	G-box binding protein MFMR
MFMR_assoc	PF16596	132 - 262	5e-21	Disordered region downstream of MFMR
bZIP_1	PF00170	282 - 343	3.2e-20	bZIP transcription factor
bZIP_2	PF07716	285 - 333	1.8e-07	Basic region leucine zipper

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004117)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46270 AT2G46270 AT2G46270 AT2G46270
fragaria_vesca	FvH4_7g15810 FvH4_7g15810 FvH4_7g15810 FvH4_7g15810
malus_domestica	MD01G1074700.v1.1 MD07G1143800.v1.1
prunus_persica	Prupe.2G182800_v2.0.a1 Prupe.2G182800_v2.0.a1 Prupe.2G182800_v2.0.a1 Prupe.2G182800_v2.0.a1 Prupe.2G182800_v2.0.a1 Prupe.2G182800_v2.0.a1 Prupe.2G182800_v2.0.a1 Prupe.2G182800_v2.0.a1
pyrus_communis	pycom01g10380 pycom07g14230
rosa_chinensis	RchiOBHm_Chr1g0358601
rosa_laevigata	RLG00000027942
rosa_multiflora	Rmu_sc0001609.1_g000020
rosa_roxburghii	Rroxscaffold_4G00297760
rosa_rugosa	Rorug01G0262800 Rorug01G0262800 Rorug01G0262800 Rorug01G0262900
rosa_samantha	Rh1AG278000 Rh1BG244500 Rh1CG261400 Rh1DG272800
rosa_wichuraiana	Rw1G024670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1106
Acc36I	ACCTGC	2 cut(s)	340, 1145
AccB1I	GGYRCC	1 cut(s)	244
AccB7I	CCANNNNNTGG	2 cut(s)	239, 1231
AccI	GTMKAC	1 cut(s)	258
AccII	CGCG	1 cut(s)	1062
AccIII	TCCGGA	1 cut(s)	820
AciI	CCGC	2 cut(s)	545, 797
AclWI	GGATC	1 cut(s)	891
AcoI	YGGCCR	1 cut(s)	184
AcsI	RAATTY	1 cut(s)	1003
AcuI	CTGAAG	2 cut(s)	528, 945
AcyI	GRCGYC	1 cut(s)	245
AfaI	GTAC	1 cut(s)	1123
AfiI	CCNNNNNNNGG	4 cut(s)	207, 239, 906, 1231
AflIII	ACRYGT	1 cut(s)	1060
AgeI	ACCGGT	1 cut(s)	990
AgsI	TTSAA	3 cut(s)	352, 412, 769
AjnI	CCWGG	5 cut(s)	622, 724, 827, 899, 1230
AloI	GAACNNNNNNTCC	2 cut(s)	496, 528
AluBI	AGCT	6 cut(s)	173, 476, 632, 650, 716, 1219
AluI	AGCT	6 cut(s)	173, 476, 632, 650, 716, 1219
Alw26I	GTCTC	2 cut(s)	491, 914
AlwI	GGATC	1 cut(s)	891
AlwNI	CAGNNNCTG	2 cut(s)	593, 653
Ama87I	CYCGRG	1 cut(s)	694
Aor13HI	TCCGGA	1 cut(s)	820
AoxI	GGCC	4 cut(s)	184, 209, 948, 1248
ApeKI	GCWGC	7 cut(s)	116, 335, 542, 619, 647, 680, 1219
ApoI	RAATTY	1 cut(s)	1003
AsiGI	ACCGGT	1 cut(s)	990
AspLEI	GCGC	1 cut(s)	247
AspS9I	GGNCC	7 cut(s)	137, 210, 320, 626, 710, 1108, 1248
AsuC2I	CCSGG	3 cut(s)	695, 696, 845
AvaI	CYCGRG	1 cut(s)	694
AvaII	GGWCC	5 cut(s)	137, 320, 626, 710, 1108
BalI	TGGCCA	1 cut(s)	186
BanI	GGYRCC	1 cut(s)	244
BbsI	GAAGAC	3 cut(s)	614, 782, 1083
BbvCI	CCTCAGC	1 cut(s)	633
BbvI	GCAGC	7 cut(s)	128, 322, 529, 631, 659, 667, 1206
BccI	CCATC	3 cut(s)	331, 416, 524
BceAI	ACGGC	1 cut(s)	449
BcgI	CGANNNNNNTGC	7 cut(s)	33, 243, 277, 860, 894, 989, 1023
BciT130I	CCWGG	5 cut(s)	624, 726, 829, 901, 1232
BciVI	GTATCC	2 cut(s)	701, 825
BcnI	CCSGG	3 cut(s)	695, 696, 845
BcoDI	GTCTC	2 cut(s)	491, 914
BfaI	CTAG	3 cut(s)	516, 891, 1022
BfmI	CTRYAG	1 cut(s)	957
BfoI	RGCGCY	1 cut(s)	248
BfuAI	ACCTGC	2 cut(s)	340, 1145
BfuI	GTATCC	2 cut(s)	701, 825
BglI	GCCNNNNNGGC	1 cut(s)	125
BisI	GCNGC	8 cut(s)	117, 336, 543, 546, 620, 648, 681, 1220
BlpI	GCTNAGC	1 cut(s)	477
BlsI	GCNGC	8 cut(s)	118, 337, 544, 547, 621, 649, 682, 1221
Bme1390I	CCNGG	8 cut(s)	624, 695, 696, 726, 829, 845, 901, 1232
Bme18I	GGWCC	5 cut(s)	137, 320, 626, 710, 1108
BmeT110I	CYCGRG	1 cut(s)	694
BmgT120I	GGNCC	7 cut(s)	137, 210, 320, 626, 710, 1108, 1248
BmiI	GGNNCC	4 cut(s)	212, 246, 321, 1109
BmrFI	CCNGG	8 cut(s)	624, 695, 696, 726, 829, 845, 901, 1232
BmsI	GCATC	6 cut(s)	7, 219, 311, 1162, 1225, 1243
BpiI	GAAGAC	3 cut(s)	614, 782, 1083
BplI	GAGNNNNNCTC	4 cut(s)	133, 165, 135, 167
BpmI	CTGGAG	1 cut(s)	618
Bpu10I	CCTNAGC	1 cut(s)	633
Bpu1102I	GCTNAGC	1 cut(s)	477
BpuEI	CTTGAG	1 cut(s)	734
BpuMI	CCSGG	3 cut(s)	695, 696, 845
BsaHI	GRCGYC	1 cut(s)	245
BsaJI	CCNNGG	3 cut(s)	307, 694, 1244
BsaWI	WCCGGW	2 cut(s)	820, 990
Bsc4I	CCNNNNNNNGG	4 cut(s)	207, 239, 906, 1231
Bse118I	RCCGGY	2 cut(s)	58, 990
Bse1I	ACTGG	2 cut(s)	789, 1044
Bse3DI	GCAATG	2 cut(s)	438, 526
BseAI	TCCGGA	1 cut(s)	820
BseBI	CCWGG	5 cut(s)	624, 726, 829, 901, 1232
BseDI	CCNNGG	3 cut(s)	307, 694, 1244
BseGI	GGATG	6 cut(s)	196, 283, 302, 323, 1177, 1240
BseLI	CCNNNNNNNGG	4 cut(s)	207, 239, 906, 1231
BseMI	GCAATG	2 cut(s)	438, 526
BseMII	CTCAG	6 cut(s)	183, 468, 624, 909, 1007, 1167
BseNI	ACTGG	2 cut(s)	789, 1044
BseRI	GAGGAG	2 cut(s)	183, 650
BseXI	GCAGC	7 cut(s)	128, 322, 529, 631, 659, 667, 1206
BsgI	GTGCAG	3 cut(s)	666, 828, 1205
Bsh1236I	CGCG	1 cut(s)	1062
BshFI	GGCC	4 cut(s)	186, 211, 950, 1250
BshNI	GGYRCC	1 cut(s)	244
BshTI	ACCGGT	1 cut(s)	990
BsiHKCI	CYCGRG	1 cut(s)	694
BsiSI	CCGG	6 cut(s)	59, 287, 695, 821, 844, 991
BslFI	GGGAC	2 cut(s)	517, 1225
BslI	CCNNNNNNNGG	4 cut(s)	207, 239, 906, 1231
BsmAI	GTCTC	2 cut(s)	491, 914
BsmFI	GGGAC	2 cut(s)	517, 1225
BsmI	GAATGC	1 cut(s)	466
BsnI	GGCC	4 cut(s)	186, 211, 950, 1250
BsoBI	CYCGRG	1 cut(s)	694
Bsp13I	TCCGGA	1 cut(s)	820
Bsp143I	GATC	2 cut(s)	64, 896
Bsp1720I	GCTNAGC	1 cut(s)	477
Bsp19I	CCATGG	1 cut(s)	307
BspACI	CCGC	2 cut(s)	545, 797
BspANI	GGCC	4 cut(s)	186, 211, 950, 1250
BspCNI	CTCAG	6 cut(s)	182, 469, 625, 910, 1008, 1168
BspEI	TCCGGA	1 cut(s)	820
BspFNI	CGCG	1 cut(s)	1062
BspHI	TCATGA	1 cut(s)	1086
BspLI	GGNNCC	4 cut(s)	212, 246, 321, 1109
BspMAI	CTGCAG	1 cut(s)	961
BspMI	ACCTGC	2 cut(s)	340, 1145
BspPI	GGATC	1 cut(s)	891
BspT107I	GGYRCC	1 cut(s)	244
BsrDI	GCAATG	2 cut(s)	438, 526
BsrFI	RCCGGY	2 cut(s)	58, 990
BsrI	ACTGG	2 cut(s)	789, 1044
BssAI	RCCGGY	2 cut(s)	58, 990
BssECI	CCNNGG	3 cut(s)	307, 694, 1244
BssMI	GATC	2 cut(s)	64, 896
BssNAI	GTATAC	1 cut(s)	259
BssNI	GRCGYC	1 cut(s)	245
BssT1I	CCWWGG	2 cut(s)	307, 1244
Bst1107I	GTATAC	1 cut(s)	259
Bst2UI	CCWGG	5 cut(s)	624, 726, 829, 901, 1232
Bst4CI	ACNGT	3 cut(s)	392, 1137, 1187
BstACI	GRCGYC	1 cut(s)	245
BstAPI	GCANNNNNTGC	2 cut(s)	229, 1262
BstC8I	GCNNGC	6 cut(s)	60, 126, 429, 915, 1060, 1267
BstDEI	CTNAG	6 cut(s)	169, 477, 633, 918, 1016, 1176
BstDSI	CCRYGG	1 cut(s)	307
BstF5I	GGATG	6 cut(s)	196, 283, 302, 323, 1177, 1240
BstFNI	CGCG	1 cut(s)	1062
BstH2I	RGCGCY	1 cut(s)	248
BstHHI	GCGC	1 cut(s)	247
BstKTI	GATC	2 cut(s)	67, 899
BstMAI	GTCTC	2 cut(s)	491, 914
BstMBI	GATC	2 cut(s)	64, 896
BstNI	CCWGG	5 cut(s)	624, 726, 829, 901, 1232
BstNSI	RCATGY	2 cut(s)	101, 619
BstSCI	CCNGG	8 cut(s)	622, 693, 694, 724, 827, 843, 899, 1230
BstSFI	CTRYAG	1 cut(s)	957
BstUI	CGCG	1 cut(s)	1062
BstV1I	GCAGC	7 cut(s)	128, 322, 529, 631, 659, 667, 1206
BstV2I	GAAGAC	3 cut(s)	614, 782, 1083
BstX2I	RGATCY	1 cut(s)	896
BstXI	CCANNNNNNTGG	2 cut(s)	500, 742
BstYI	RGATCY	1 cut(s)	896
BstZ17I	GTATAC	1 cut(s)	259
BsuI	GTATCC	2 cut(s)	701, 825
BsuRI	GGCC	4 cut(s)	186, 211, 950, 1250
BtgI	CCRYGG	1 cut(s)	307
BtsCI	GGATG	6 cut(s)	196, 283, 302, 323, 1177, 1240
BtsI	GCAGTG	2 cut(s)	451, 591
BtsIMutI	CAGTG	6 cut(s)	451, 591, 796, 971, 1013, 1192
BveI	ACCTGC	2 cut(s)	340, 1145
Cac8I	GCNNGC	6 cut(s)	60, 126, 429, 915, 1060, 1267
CaiI	CAGNNNCTG	2 cut(s)	593, 653
CciI	TCATGA	1 cut(s)	1086
CfoI	GCGC	1 cut(s)	247
Cfr10I	RCCGGY	2 cut(s)	58, 990
Cfr13I	GGNCC	7 cut(s)	137, 210, 320, 626, 710, 1108, 1248
Cfr9I	CCCGGG	1 cut(s)	694
Csp6I	GTAC	1 cut(s)	1122
CspAI	ACCGGT	1 cut(s)	990
CviAII	CATG	7 cut(s)	98, 121, 266, 308, 616, 730, 1087
CviQI	GTAC	1 cut(s)	1122
DdeI	CTNAG	6 cut(s)	169, 477, 633, 918, 1016, 1176
DinI	GGCGCC	1 cut(s)	246
DpnI	GATC	2 cut(s)	66, 898
DpnII	GATC	2 cut(s)	64, 896
DrdI	GACNNNNNNGTC	1 cut(s)	1106
DseDI	GACNNNNNNGTC	1 cut(s)	1106
EaeI	YGGCCR	1 cut(s)	184
Eco130I	CCWWGG	2 cut(s)	307, 1244
Eco147I	AGGCCT	1 cut(s)	950
Eco47I	GGWCC	5 cut(s)	137, 320, 626, 710, 1108
Eco57I	CTGAAG	2 cut(s)	528, 945
Eco88I	CYCGRG	1 cut(s)	694
EcoRI	GAATTC	1 cut(s)	1003
EcoRII	CCWGG	5 cut(s)	622, 724, 827, 899, 1230
EcoT14I	CCWWGG	2 cut(s)	307, 1244
EgeI	GGCGCC	1 cut(s)	246
EheI	GGCGCC	1 cut(s)	246
ErhI	CCWWGG	2 cut(s)	307, 1244
FaeI	CATG	7 cut(s)	101, 124, 269, 311, 619, 733, 1090
FaqI	GGGAC	2 cut(s)	517, 1225
FatI	CATG	7 cut(s)	97, 120, 265, 307, 615, 729, 1086
FauNDI	CATATG	1 cut(s)	225
FblI	GTMKAC	1 cut(s)	258
Fnu4HI	GCNGC	8 cut(s)	117, 336, 543, 546, 620, 648, 681, 1220
FokI	GGATG	6 cut(s)	183, 270, 289, 310, 1184, 1247
Fsp4HI	GCNGC	8 cut(s)	117, 336, 543, 546, 620, 648, 681, 1220
FspBI	CTAG	3 cut(s)	516, 891, 1022
GlaI	GCGC	1 cut(s)	246
GluI	GCNGC	8 cut(s)	117, 336, 543, 546, 620, 648, 681, 1220
GsuI	CTGGAG	1 cut(s)	618
HaeII	RGCGCY	1 cut(s)	248
HaeIII	GGCC	4 cut(s)	186, 211, 950, 1250
HapII	CCGG	6 cut(s)	59, 287, 695, 821, 844, 991
HhaI	GCGC	1 cut(s)	247
Hin1I	GRCGYC	1 cut(s)	245
Hin1II	CATG	7 cut(s)	101, 124, 269, 311, 619, 733, 1090
Hin6I	GCGC	1 cut(s)	245
HinP1I	GCGC	1 cut(s)	245
HincII	GTYRAC	2 cut(s)	996, 1147
HindII	GTYRAC	2 cut(s)	996, 1147
HinfI	GANTC	4 cut(s)	73, 343, 884, 1240
HpaI	GTTAAC	2 cut(s)	996, 1147
HpaII	CCGG	6 cut(s)	59, 287, 695, 821, 844, 991
Hpy166II	GTNNAC	3 cut(s)	259, 996, 1147
Hpy188I	TCNGA	4 cut(s)	37, 87, 141, 982
Hpy188III	TCNNGA	8 cut(s)	107, 575, 751, 766, 821, 881, 1087, 1140
Hpy8I	GTNNAC	3 cut(s)	259, 996, 1147
HpyAV	CCTTC	2 cut(s)	503, 596
HpyCH4III	ACNGT	3 cut(s)	392, 1137, 1187
HpyF3I	CTNAG	6 cut(s)	169, 477, 633, 918, 1016, 1176
Hsp92I	GRCGYC	1 cut(s)	245
Hsp92II	CATG	7 cut(s)	101, 124, 269, 311, 619, 733, 1090
HspAI	GCGC	1 cut(s)	245
KasI	GGCGCC	1 cut(s)	244
Kpn2I	TCCGGA	1 cut(s)	820
KroI	GCCGGC	1 cut(s)	58
KroNI	GCCGGC	1 cut(s)	60
KspAI	GTTAAC	2 cut(s)	996, 1147
Kzo9I	GATC	2 cut(s)	64, 896
LmnI	GCTCC	1 cut(s)	473
Lsp1109I	GCAGC	7 cut(s)	128, 322, 529, 631, 659, 667, 1206
LweI	GCATC	6 cut(s)	7, 219, 311, 1162, 1225, 1243
MaeI	CTAG	3 cut(s)	516, 891, 1022
MaeIII	GTNAC	3 cut(s)	340, 670, 1187
MalI	GATC	2 cut(s)	66, 898
MboI	GATC	2 cut(s)	64, 896
MboII	GAAGA	7 cut(s)	573, 619, 787, 906, 938, 1080, 1088
MflI	RGATCY	1 cut(s)	896
MlsI	TGGCCA	1 cut(s)	186
MluCI	AATT	2 cut(s)	701, 1003
MluI	ACGCGT	1 cut(s)	1060
MluNI	TGGCCA	1 cut(s)	186
Mly113I	GGCGCC	1 cut(s)	245
MlyI	GAGTC	2 cut(s)	337, 1249
MmeI	TCCRAC	3 cut(s)	688, 723, 1135
Mox20I	TGGCCA	1 cut(s)	186
MroI	TCCGGA	1 cut(s)	820
MroNI	GCCGGC	1 cut(s)	58
MscI	TGGCCA	1 cut(s)	186
MseI	TTAA	4 cut(s)	30, 398, 995, 1146
MslI	CAYNNNNRTG	2 cut(s)	270, 1085
Msp20I	TGGCCA	1 cut(s)	186
MspA1I	CMGCKG	2 cut(s)	632, 716
MspI	CCGG	6 cut(s)	59, 287, 695, 821, 844, 991
MspR9I	CCNGG	8 cut(s)	624, 695, 696, 726, 829, 845, 901, 1232
Mva1269I	GAATGC	1 cut(s)	466
MvaI	CCWGG	5 cut(s)	624, 726, 829, 901, 1232
MvnI	CGCG	1 cut(s)	1062
NaeI	GCCGGC	1 cut(s)	60
NarI	GGCGCC	1 cut(s)	245
NciI	CCSGG	3 cut(s)	695, 696, 845
NcoI	CCATGG	1 cut(s)	307
NdeI	CATATG	1 cut(s)	225
NdeII	GATC	2 cut(s)	64, 896
NgoMIV	GCCGGC	1 cut(s)	58
NlaIII	CATG	7 cut(s)	101, 124, 269, 311, 619, 733, 1090
NlaIV	GGNNCC	4 cut(s)	212, 246, 321, 1109
NmuCI	GTSAC	2 cut(s)	340, 1187
NspI	RCATGY	2 cut(s)	101, 619
PagI	TCATGA	1 cut(s)	1086
PceI	AGGCCT	1 cut(s)	950
PctI	GAATGC	1 cut(s)	466
PdiI	GCCGGC	1 cut(s)	60
PfeI	GAWTC	2 cut(s)	73, 884
PflMI	CCANNNNNTGG	2 cut(s)	239, 1231
PfoI	TCCNGGA	1 cut(s)	1230
PinAI	ACCGGT	1 cut(s)	990
PkrI	GCNGC	8 cut(s)	118, 337, 544, 547, 621, 649, 682, 1221
PleI	GAGTC	2 cut(s)	337, 1248
PluTI	GGCGCC	1 cut(s)	248
PpsI	GAGTC	2 cut(s)	337, 1248
Psp6I	CCWGG	5 cut(s)	622, 724, 827, 899, 1230
PspGI	CCWGG	5 cut(s)	622, 724, 827, 899, 1230
PspN4I	GGNNCC	4 cut(s)	212, 246, 321, 1109
PspPI	GGNCC	7 cut(s)	137, 210, 320, 626, 710, 1108, 1248
PstI	CTGCAG	1 cut(s)	961
PstNI	CAGNNNCTG	2 cut(s)	593, 653
PsuI	RGATCY	1 cut(s)	896
PvuII	CAGCTG	2 cut(s)	632, 716
RsaI	GTAC	1 cut(s)	1123
RsaNI	GTAC	1 cut(s)	1122
RseI	CAYNNNNRTG	2 cut(s)	270, 1085
SaqAI	TTAA	4 cut(s)	30, 398, 995, 1146
SatI	GCNGC	8 cut(s)	117, 336, 543, 546, 620, 648, 681, 1220
Sau3AI	GATC	2 cut(s)	64, 896
Sau96I	GGNCC	7 cut(s)	137, 210, 320, 626, 710, 1108, 1248
SchI	GAGTC	2 cut(s)	337, 1249
ScrFI	CCNGG	8 cut(s)	624, 695, 696, 726, 829, 845, 901, 1232
SfaNI	GCATC	6 cut(s)	7, 219, 311, 1162, 1225, 1243
SfcI	CTRYAG	1 cut(s)	957
SfoI	GGCGCC	1 cut(s)	246
SinI	GGWCC	5 cut(s)	137, 320, 626, 710, 1108
SmaI	CCCGGG	1 cut(s)	696
SmiMI	CAYNNNNRTG	2 cut(s)	270, 1085
SmlI	CTYRAG	1 cut(s)	749
SmoI	CTYRAG	1 cut(s)	749
Sse9I	AATT	2 cut(s)	701, 1003
SseBI	AGGCCT	1 cut(s)	950
SsiI	CCGC	2 cut(s)	545, 797
SspDI	GGCGCC	1 cut(s)	244
SspMI	CTAG	3 cut(s)	516, 891, 1022
StuI	AGGCCT	1 cut(s)	950
StyD4I	CCNGG	8 cut(s)	622, 693, 694, 724, 827, 843, 899, 1230
StyI	CCWWGG	2 cut(s)	307, 1244
TaaI	ACNGT	3 cut(s)	392, 1137, 1187
TaqI	TCGA	2 cut(s)	773, 945
TasI	AATT	2 cut(s)	701, 1003
TauI	GCSGC	1 cut(s)	548
TfiI	GAWTC	2 cut(s)	73, 884
Tru1I	TTAA	4 cut(s)	30, 398, 995, 1146
Tru9I	TTAA	4 cut(s)	30, 398, 995, 1146
TscAI	CASTG	6 cut(s)	451, 598, 796, 978, 1020, 1192
TseFI	GTSAC	2 cut(s)	340, 1187
TseI	GCWGC	7 cut(s)	116, 335, 542, 619, 647, 680, 1219
Tsp45I	GTSAC	2 cut(s)	340, 1187
TspDTI	ATGAA	3 cut(s)	416, 473, 1103
TspMI	CCCGGG	1 cut(s)	694
TspRI	CASTG	6 cut(s)	451, 598, 796, 978, 1020, 1192
Van91I	CCANNNNNTGG	2 cut(s)	239, 1231
VpaK11BI	GGWCC	5 cut(s)	137, 320, 626, 710, 1108
XapI	RAATTY	1 cut(s)	1003
XceI	RCATGY	2 cut(s)	101, 619
XmaI	CCCGGG	1 cut(s)	694
XmiI	GTMKAC	1 cut(s)	258
XspI	CTAG	3 cut(s)	516, 891, 1022

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.