Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011959

Physical Location & Seq

Genomic Coordinates Forward (+)

18948278 .. 18953116

Gene Structure 4839 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1083100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2928 bp

ATGGAATGGAATCCCGAAACCCTACAGCACCTCTCTCAGTGCTTCCTCCACACCCTCTCTCCGGCCCCGGAGCCTCGCCGCCGCGCCGAGGCTTCACTATCCGAGGCATCCCTCCACCCCAATTACGGCCTCGCCGTCCTCCGCCTCGTCGCCGAGCCCACCGTTGATGATCAGATCCGGCAGGCCGCTTCCGTCAACTTCAAAAACCACCTCAAGGCGCGATGGGCTCCCAACCCCTCTTCCGACGAGATCCCCATCCCCGACGCCGAGAAGGACCAGATCAAGGGCCTTATCGTCTCCCTCATGCTCTCCGCGACACCCAAGATTCAGGGCCAGCTCAGCGAAGCCCTAGTCCTCATTGGCAAGCACGATTTCCCCAAGTTGTGGCCCGCTTTGCTCCCCGAGCTCATCTCCAGCCTTGGGGCGGCTTCGCTTGCTGGCGACTACGCCTCCATTAACGGTATTCTCGGCACTGCCAACTCCATTTTTAAGAAATTTCGTTATCAGTACAAGACCAATGATCTTTTGCTTGATTTGAAATACTGTTTGGATAATTTCGCCGCACCCCTTTTGGAAATTTTCATGAAAACTGCAAACTTGATCGAGTCTGCTGCTAATTCCGGAGGGTCTGCCGTGGTGCTTAAGCCCCTTTTTGACTCACAGAGATTGTGTTGTAGGATTTTCTACTCATTGAATTTTCAAGAGTTGCCTGAGTTTTTTGAGGACCATATGAATGAGTGGATGACTGAGATGAAAAAATATTTAACCACTAGTTATCCAGCGCTGGAGAGTAGCGCTGATGGGCTTGCGCTGGTCGATGGGTTGCGTGCAGCTGTTTGCGAGAATATTAACCTTTACATGGAAAAGAATGAGGAAGAATTTCAGGTGTACTTGAATGATTTTGCGCTCGCTGTCTGGCAATTATTAGTGAATGTGTCTCAAGTGTCGAGCCGTGATCAGTTGGCTGTTACAGCTATTAAGTTCTTGACCACAGTTAGCACAAGTGTGCATCATAATCTTTTTGCTGCTGAGGGTGTGATACCACAGATTTGTCAGGGCATTGTGATCCCTAATGTGAGACTGAGGGAGGAGGATGAGGAATTATTTGAAATGAACTATATTGAGTTCGTCAGGAGGGATATGGAGGGTAGTGATCTCGATACTAGGAGGAGGATTGCATGTGAGCTTCTTAAAGGGATCGCTAGCAATTATAAACAACAGGTTACCAATTTGGTTTCTCTGCAGATACAGAGTTTGCTAAGCTCTTTTGCTACAAACCCAGTTGTGAATTGGAAGGATAAGGACTGTGCTATATACTTGGTTGTATCACTTGCTATTAAGAAGGCTGGTGGTACTTCTGTTTCGACTGATCTTGTTGATGTTCAGAGCTTCTTTGGGACGGTAATTGCTCCAGAATTGCAGAGTCAGGATGTCAATGGGTTTCCGATGCTCAAGGCCGGTGCACTGAAGTTTTTCACAATGTTCAGGAATCACATACCAAAGCCTATGGCATTGCAATTTTTTCCAGATTTGATTCGGTTCCTTCGTGCAGAGTCAAATGTTGTTCACTCCTATGCTGCAAGTTGTATTGAGAAACTTTTGATGGTAAAGGACGATGGTGGAAGAGCAAGATACACTTCAACAGATATTTCTCCTGTTCTACCACAACTGATGAACAATCTCTTTGAGGCCTTGAAGGTTCCAGAATCTGAGGAAAACCAATACATAATGAAGTGTATTATGCGAGTTCTCGGGGTTGCAGACATATCGTTTGAGATTGCTGATCCTTGCATTAAAGGGTTTACATTAATACTCAACAAAGCTTGTGAAAACCCCAAGAATCCAATATTTAACCACTATCTTTTTGAGTCGGTGGCTGTTCTTGTGAAGCGTGCATGTGGGAAGAATGCCTCTCTAATATCACTTTTTGAGACGAGCCTATTTCCCAGCCTCCAGAAGATACTGGGGGAAGATGTGACCGAGTTCTTTCCTTATGCATTTCAGCTGCTTGCTCAGCTAGTTGAGTTGAATAACCCGCCAATCTCACCGTCTTACATGCATATTTTTGAGATACTTTTGTCCCCTGATTTATGGAAAAAGGCGTCTAATGTTCCAGCTCTTGTGCGTTTGCTTCAAGCATTCCTTTACAAGGCACCCCATGAGCTCAACCAAGGGGGGAGGTTGCAGCAAGTGCTTGTGATATTTAATAAGCTTGTCTCAGCTCGTAGCACAGATGAACAGGGTTTCTATGTGCTAAATACTATTATCGACAGCCTTGAGTATAATGTGATTGCGCCTTACATTGGTGGCATTTGGAGTGCCCTTTTCACGGTGCTCCAAACCAGGCAAACAGGAAAGTTTATTAAGTCTCTCTTGATTTTTATGTCACTCTTTCTGGTCAAACACGGCTCTCAAAACCTTGCAGATACAATGAATGCTGTTCAGGGCAACATATTTCAGGTCATCTTGGTGCAGTTTTGGATATCCAATCTTAAGCTTATTACAGGAGTCATTGAGACTAAGTTGACTGCTGTTGCATCAACCAGACTTCTATGTGAATCTCCAGCACTTCTGGATGCCGCAGCTGTTGAACACTGGGGGAAGATGCTGGACAGCATTATGACCCTTCTTTCGCGGCCTGAACAGGACAGGGTTGAAGAGGATCCAGAAATGCCTGATATTGCTGAAAACACAGGTTACTCTGCCACCTTTGTTCGTCTCCACAATGCTGGGAAGAGAGAGGATGACCCTCTGAAAGATATAAGGGATCCGAAAGAATTTTTGGTAACTTCATTGGCTGGGTTTTCCAAACTTTCCCCTAACAGATACCCTCAAATCATCAGTCAGTATCTTGACCCAGCCAATCAGGCAGAACTACGTCGTCTCTGCGAATTTTATAATTGCCCGATTGATTTCACATGGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005049 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0005911 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0009506 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0030054 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0140104 GO:0140142

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CAS_CSE1 Cse1 IBN_N

Protein Analysis

976

Amino Acids

109.4

Weight (kDa)

5.4

Isoelectric Point (pI)

45.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	29 - 104	1.4e-12	Importin-beta N-terminal domain
Cse1	PF08506	162 - 530	8.7e-137	Cse1
CAS_CSE1	PF03378	531 - 960	2e-156	CAS/CSE protein, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46520
fragaria_vesca	FvH4_7g16830 FvH4_7g16830
malus_domestica	MD01G1083100.v1.1
prunus_persica	Prupe.2G190300_v2.0.a1 Prupe.2G190300_v2.0.a1 Prupe.2G190300_v2.0.a1 Prupe.2G190300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0360031
rosa_laevigata	RLG00000027837
rosa_multiflora	Rmu_co8506667.1_g000001 Rmu_ssc0000368.1_g000034
rosa_roxburghii	Rroxscaffold_4G00296440
rosa_rugosa	Rorug01G0274600
rosa_samantha	Rh1AG288500 Rh1BG254000 Rh1CG271500 Rh1DG283100
rosa_wichuraiana	Rw1G025580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1212, 2898
AccB1I	GGYRCC	1 cut(s)	2152
AccB7I	CCANNNNNTGG	1 cut(s)	384
AccII	CGCG	4 cut(s)	84, 220, 314, 2635
AccIII	TCCGGA	1 cut(s)	620
AclWI	GGATC	9 cut(s)	169, 244, 1060, 1205, 1778, 2657, 2670, 2762, 2775
AcsI	RAATTY	6 cut(s)	494, 576, 694, 878, 2777, 2891
AcuI	CTGAAG	1 cut(s)	1487
AcyI	GRCGYC	2 cut(s)	264, 2102
AfaI	GTAC	3 cut(s)	509, 890, 1354
AfeI	AGCGCT	2 cut(s)	783, 796
AflII	CTTAAG	2 cut(s)	641, 2492
AhlI	ACTAGT	1 cut(s)	770
AjnI	CCWGG	1 cut(s)	2342
AjuI	GAANNNNNNNTTGG	4 cut(s)	530, 562, 2765, 2797
AleI	CACNNNNGTG	1 cut(s)	1004
Alw21I	GWGCWC	4 cut(s)	408, 1465, 2166, 2337
Alw26I	GTCTC	9 cut(s)	301, 942, 1072, 1925, 2221, 2373, 2510, 2723, 2888
Alw44I	GTGCAC	1 cut(s)	1461
AlwI	GGATC	9 cut(s)	169, 244, 1060, 1205, 1778, 2657, 2670, 2762, 2775
AlwNI	CAGNNNCTG	1 cut(s)	1709
Ama87I	CYCGRG	2 cut(s)	401, 1751
Aor13HI	TCCGGA	1 cut(s)	620
Aor51HI	AGCGCT	2 cut(s)	783, 796
AoxI	GGCC	9 cut(s)	63, 127, 183, 286, 331, 386, 1455, 1689, 2636
ApaLI	GTGCAC	1 cut(s)	1461
ApeKI	GCWGC	7 cut(s)	611, 830, 1025, 1577, 2005, 2185, 2582
ApoI	RAATTY	6 cut(s)	494, 576, 694, 878, 2777, 2891
ArsI	GACNNNNNNTTYG	8 cut(s)	336, 368, 1754, 1786, 2614, 2646, 2827, 2859
AseI	ATTAAT	1 cut(s)	1808
Asp700I	GAANNNNTTC	2 cut(s)	879, 1472
AspLEI	GCGC	7 cut(s)	86, 220, 784, 797, 811, 907, 2296
AspS9I	GGNCC	6 cut(s)	64, 274, 286, 331, 387, 724
AsuC2I	CCSGG	1 cut(s)	68
AsuHPI	GGTGA	1 cut(s)	2037
AsuNHI	GCTAGC	1 cut(s)	1202
AvaI	CYCGRG	2 cut(s)	401, 1751
AvaII	GGWCC	2 cut(s)	274, 724
BaeGI	GKGCMC	2 cut(s)	1465, 2323
BaeI	ACNNNNGTAYC	4 cut(s)	1344, 1377, 2063, 2096
BamHI	GGATCC	2 cut(s)	2662, 2767
BanI	GGYRCC	1 cut(s)	2152
BanII	GRGCYC	4 cut(s)	159, 229, 408, 2166
Bbv12I	GWGCWC	4 cut(s)	408, 1465, 2166, 2337
BbvCI	CCTCAGC	1 cut(s)	1029
BbvI	GCAGC	7 cut(s)	598, 842, 1012, 1564, 1992, 2197, 2594
BccI	CCATC	6 cut(s)	216, 263, 794, 812, 1597, 1610
BceAI	ACGGC	5 cut(s)	119, 142, 617, 936, 2422
BcgI	CGANNNNNNTGC	4 cut(s)	593, 627, 1749, 1783
BciT130I	CCWGG	1 cut(s)	2344
BclI	TGATCA	2 cut(s)	169, 955
BcnI	CCSGG	1 cut(s)	68
BcoDI	GTCTC	9 cut(s)	301, 942, 1072, 1925, 2221, 2373, 2510, 2723, 2888
BcuI	ACTAGT	1 cut(s)	770
BfaI	CTAG	5 cut(s)	350, 771, 1164, 1203, 2018
BfmI	CTRYAG	2 cut(s)	23, 1241
BfoI	RGCGCY	2 cut(s)	785, 798
BfrI	CTTAAG	2 cut(s)	641, 2492
BglI	GCCNNNNNGGC	1 cut(s)	2867
BlpI	GCTNAGC	3 cut(s)	338, 1259, 2013
Bme1390I	CCNGG	2 cut(s)	68, 2344
Bme18I	GGWCC	2 cut(s)	274, 724
BmeT110I	CYCGRG	2 cut(s)	401, 1751
BmgT120I	GGNCC	6 cut(s)	64, 274, 286, 331, 387, 724
BmiI	GGNNCC	8 cut(s)	66, 72, 228, 1541, 1701, 2154, 2664, 2769
BmrFI	CCNGG	2 cut(s)	68, 2344
BmrI	ACTGGG	3 cut(s)	1274, 1973, 2605
BmsI	GCATC	6 cut(s)	116, 1018, 1437, 2546, 2566, 2595
BmtI	GCTAGC	1 cut(s)	1206
BmuI	ACTGGG	3 cut(s)	1274, 1973, 2605
BplI	GAGNNNNNCTC	2 cut(s)	395, 427
BpmI	CTGGAG	5 cut(s)	397, 806, 1395, 1937, 2547
Bpu10I	CCTNAGC	1 cut(s)	1029
Bpu1102I	GCTNAGC	3 cut(s)	338, 1259, 2013
BpuEI	CTTGAG	4 cut(s)	197, 924, 1436, 2297
BpuMI	CCSGG	1 cut(s)	68
BsaBI	GATNNNNATC	1 cut(s)	254
BsaHI	GRCGYC	2 cut(s)	264, 2102
BsaJI	CCNNGG	6 cut(s)	66, 87, 102, 418, 633, 2170
BsaWI	WCCGGW	1 cut(s)	620
BsaXI	ACNNNNNCTCC	4 cut(s)	43, 73, 434, 464
Bse118I	RCCGGY	1 cut(s)	1457
Bse1I	ACTGG	3 cut(s)	1280, 1968, 2600
Bse3DI	GCAATG	1 cut(s)	1511
Bse8I	GATNNNNATC	1 cut(s)	254
BseAI	TCCGGA	1 cut(s)	620
BseBI	CCWGG	1 cut(s)	2344
BseDI	CCNNGG	6 cut(s)	66, 87, 102, 418, 633, 2170
BseGI	GGATG	7 cut(s)	107, 255, 747, 1099, 1435, 2581, 2749
BseJI	GATNNNNATC	1 cut(s)	254
BseMI	GCAATG	1 cut(s)	1511
BseMII	CTCAG	9 cut(s)	50, 352, 702, 738, 1020, 1073, 1701, 2027, 2232
BseNI	ACTGG	3 cut(s)	1280, 1968, 2600
BseRI	GAGGAG	2 cut(s)	1103, 1183
BseSI	GKGCMC	2 cut(s)	1465, 2323
BseXI	GCAGC	7 cut(s)	598, 842, 1012, 1564, 1992, 2197, 2594
BseYI	CCCAGC	4 cut(s)	1946, 2729, 2798, 2857
BsgI	GTGCAG	3 cut(s)	849, 1569, 2492
Bsh1236I	CGCG	4 cut(s)	84, 220, 314, 2635
BshFI	GGCC	9 cut(s)	65, 129, 185, 288, 333, 388, 1457, 1691, 2638
BshNI	GGYRCC	1 cut(s)	2152
BsiHKAI	GWGCWC	4 cut(s)	408, 1465, 2166, 2337
BsiHKCI	CYCGRG	2 cut(s)	401, 1751
BsiSI	CCGG	5 cut(s)	62, 68, 178, 621, 1458
BslFI	GGGAC	2 cut(s)	1411, 2065
BsmAI	GTCTC	9 cut(s)	301, 942, 1072, 1925, 2221, 2373, 2510, 2723, 2888
BsmBI	CGTCTC	4 cut(s)	301, 1925, 2723, 2888
BsmFI	GGGAC	2 cut(s)	1411, 2065
BsmI	GAATGC	3 cut(s)	1912, 2138, 2440
BsnI	GGCC	9 cut(s)	65, 129, 185, 288, 333, 388, 1457, 1691, 2638
BsoBI	CYCGRG	2 cut(s)	401, 1751
Bsp1286I	GDGCHC	7 cut(s)	159, 229, 408, 1465, 2166, 2323, 2337
Bsp13I	TCCGGA	1 cut(s)	620
Bsp1720I	GCTNAGC	3 cut(s)	338, 1259, 2013
BspANI	GGCC	9 cut(s)	65, 129, 185, 288, 333, 388, 1457, 1691, 2638
BspCNI	CTCAG	9 cut(s)	49, 351, 703, 739, 1021, 1074, 1702, 2026, 2231
BspEI	TCCGGA	1 cut(s)	620
BspFNI	CGCG	4 cut(s)	84, 220, 314, 2635
BspHI	TCATGA	1 cut(s)	582
BspLI	GGNNCC	8 cut(s)	66, 72, 228, 1541, 1701, 2154, 2664, 2769
BspMAI	CTGCAG	1 cut(s)	1245
BspOI	GCTAGC	1 cut(s)	1206
BspPI	GGATC	9 cut(s)	169, 244, 1060, 1205, 1778, 2657, 2670, 2762, 2775
BspQI	GCTCTTC	1 cut(s)	1618
BspT107I	GGYRCC	1 cut(s)	2152
BspTI	CTTAAG	2 cut(s)	641, 2492
BsrDI	GCAATG	1 cut(s)	1511
BsrFI	RCCGGY	1 cut(s)	1457
BsrI	ACTGG	3 cut(s)	1280, 1968, 2600
BssAI	RCCGGY	1 cut(s)	1457
BssECI	CCNNGG	6 cut(s)	66, 87, 102, 418, 633, 2170
BssNI	GRCGYC	2 cut(s)	264, 2102
BssT1I	CCWWGG	2 cut(s)	418, 2170
Bst2UI	CCWGG	1 cut(s)	2344
Bst4CI	ACNGT	8 cut(s)	163, 461, 545, 994, 1307, 1402, 2049, 2332
Bst6I	CTCTTC	4 cut(s)	244, 1618, 2652, 2729
BstACI	GRCGYC	2 cut(s)	264, 2102
BstAFI	CTTAAG	2 cut(s)	641, 2492
BstAPI	GCANNNNNTGC	1 cut(s)	2191
BstDSI	CCRYGG	1 cut(s)	633
BstEII	GGTNACC	1 cut(s)	1222
BstENI	CCTNNNNNAGG	1 cut(s)	2147
BstF5I	GGATG	7 cut(s)	107, 255, 747, 1099, 1435, 2581, 2749
BstFNI	CGCG	4 cut(s)	84, 220, 314, 2635
BstH2I	RGCGCY	2 cut(s)	785, 798
BstHHI	GCGC	7 cut(s)	86, 220, 784, 797, 811, 907, 2296
BstMAI	GTCTC	9 cut(s)	301, 942, 1072, 1925, 2221, 2373, 2510, 2723, 2888
BstMWI	GCNNNNNNNGC	9 cut(s)	224, 339, 394, 403, 434, 971, 2014, 2191, 2867
BstNI	CCWGG	1 cut(s)	2344
BstNSI	RCATGY	3 cut(s)	1182, 1899, 2059
BstPI	GGTNACC	1 cut(s)	1222
BstSCI	CCNGG	2 cut(s)	66, 2342
BstSFI	CTRYAG	2 cut(s)	23, 1241
BstSLI	GKGCMC	2 cut(s)	1465, 2323
BstUI	CGCG	4 cut(s)	84, 220, 314, 2635
BstV1I	GCAGC	7 cut(s)	598, 842, 1012, 1564, 1992, 2197, 2594
BstX2I	RGATCY	4 cut(s)	174, 249, 2662, 2767
BstXI	CCANNNNNNTGG	1 cut(s)	2729
BstYI	RGATCY	4 cut(s)	174, 249, 2662, 2767
BsuRI	GGCC	9 cut(s)	65, 129, 185, 288, 333, 388, 1457, 1691, 2638
BtgI	CCRYGG	1 cut(s)	633
BtgZI	GCGATG	1 cut(s)	235
BtsCI	GGATG	7 cut(s)	107, 255, 747, 1099, 1435, 2581, 2749
BtsI	GCAGTG	1 cut(s)	471
BtsIMutI	CAGTG	4 cut(s)	44, 471, 1463, 2593
CaiI	CAGNNNCTG	1 cut(s)	1709
CciI	TCATGA	1 cut(s)	582
CfoI	GCGC	7 cut(s)	86, 220, 784, 797, 811, 907, 2296
Cfr10I	RCCGGY	1 cut(s)	1457
Cfr13I	GGNCC	6 cut(s)	64, 274, 286, 331, 387, 724
CseI	GACGC	2 cut(s)	272, 2091
Csp6I	GTAC	3 cut(s)	508, 889, 1353
CviAII	CATG	8 cut(s)	304, 583, 859, 1179, 1896, 2056, 2159, 2919
CviQI	GTAC	3 cut(s)	508, 889, 1353
Eam1104I	CTCTTC	4 cut(s)	244, 1618, 2652, 2729
EarI	CTCTTC	4 cut(s)	244, 1618, 2652, 2729
EciI	GGCGGA	1 cut(s)	131
Ecl136II	GAGCTC	2 cut(s)	406, 2164
Eco130I	CCWWGG	2 cut(s)	418, 2170
Eco147I	AGGCCT	1 cut(s)	1691
Eco24I	GRGCYC	4 cut(s)	159, 229, 408, 2166
Eco32I	GATATC	1 cut(s)	2484
Eco47I	GGWCC	2 cut(s)	274, 724
Eco47III	AGCGCT	2 cut(s)	783, 796
Eco53kI	GAGCTC	2 cut(s)	406, 2164
Eco57I	CTGAAG	1 cut(s)	1487
Eco88I	CYCGRG	2 cut(s)	401, 1751
Eco91I	GGTNACC	1 cut(s)	1222
EcoICRI	GAGCTC	2 cut(s)	406, 2164
EcoNI	CCTNNNNNAGG	1 cut(s)	2147
EcoO109I	RGGNCCY	1 cut(s)	286
EcoO65I	GGTNACC	1 cut(s)	1222
EcoRII	CCWGG	1 cut(s)	2342
EcoRV	GATATC	1 cut(s)	2484
EcoT14I	CCWWGG	2 cut(s)	418, 2170
EcoT22I	ATGCAT	2 cut(s)	1999, 2061
EcoT38I	GRGCYC	4 cut(s)	159, 229, 408, 2166
ErhI	CCWWGG	2 cut(s)	418, 2170
Esp3I	CGTCTC	4 cut(s)	301, 1925, 2723, 2888
FaeI	CATG	8 cut(s)	307, 586, 862, 1182, 1899, 2059, 2162, 2922
FalI	AAGNNNNNCTT	6 cut(s)	1621, 1653, 2127, 2159, 2357, 2389
FaqI	GGGAC	2 cut(s)	1411, 2065
FatI	CATG	8 cut(s)	303, 582, 858, 1178, 1895, 2055, 2158, 2918
FauI	CCCGC	2 cut(s)	397, 2043
FauNDI	CATATG	1 cut(s)	729
FbaI	TGATCA	2 cut(s)	169, 955
FokI	GGATG	7 cut(s)	94, 242, 754, 1106, 1442, 2588, 2756
FriOI	GRGCYC	4 cut(s)	159, 229, 408, 2166
FspBI	CTAG	5 cut(s)	350, 771, 1164, 1203, 2018
GlaI	GCGC	7 cut(s)	85, 219, 783, 796, 810, 906, 2295
GsaI	CCCAGC	4 cut(s)	1950, 2733, 2802, 2861
GsuI	CTGGAG	5 cut(s)	397, 806, 1395, 1937, 2547
HaeII	RGCGCY	2 cut(s)	785, 798
HaeIII	GGCC	9 cut(s)	65, 129, 185, 288, 333, 388, 1457, 1691, 2638
HapII	CCGG	5 cut(s)	62, 68, 178, 621, 1458
HgaI	GACGC	2 cut(s)	272, 2091
HhaI	GCGC	7 cut(s)	86, 220, 784, 797, 811, 907, 2296
Hin1I	GRCGYC	2 cut(s)	264, 2102
Hin1II	CATG	8 cut(s)	307, 586, 862, 1182, 1899, 2059, 2162, 2922
Hin6I	GCGC	7 cut(s)	84, 218, 782, 795, 809, 905, 2294
HinP1I	GCGC	7 cut(s)	84, 218, 782, 795, 809, 905, 2294
HincII	GTYRAC	2 cut(s)	196, 2526
HindII	GTYRAC	2 cut(s)	196, 2526
HindIII	AAGCTT	3 cut(s)	1821, 2210, 2495
HpaII	CCGG	5 cut(s)	62, 68, 178, 621, 1458
HphI	GGTGA	1 cut(s)	2037
Hpy166II	GTNNAC	6 cut(s)	196, 889, 1463, 1567, 1803, 2526
Hpy188I	TCNGA	8 cut(s)	103, 174, 244, 1386, 1446, 1711, 2754, 2772
Hpy8I	GTNNAC	6 cut(s)	196, 889, 1463, 1567, 1803, 2526
Hpy99I	CGWCG	4 cut(s)	152, 248, 266, 2883
HpyAV	CCTTC	6 cut(s)	265, 1288, 1336, 1553, 1690, 2636
HpyCH4III	ACNGT	8 cut(s)	163, 461, 545, 994, 1307, 1402, 2049, 2332
HpyCH4IV	ACGT	1 cut(s)	2878
HpyF10VI	GCNNNNNNNGC	9 cut(s)	224, 339, 394, 403, 434, 971, 2014, 2191, 2867
HpySE526I	ACGT	1 cut(s)	2878
Hsp92I	GRCGYC	2 cut(s)	264, 2102
Hsp92II	CATG	8 cut(s)	307, 586, 862, 1182, 1899, 2059, 2162, 2922
HspAI	GCGC	7 cut(s)	84, 218, 782, 795, 809, 905, 2294
Kpn2I	TCCGGA	1 cut(s)	620
Ksp22I	TGATCA	2 cut(s)	169, 955
LguI	GCTCTTC	1 cut(s)	1618
LmnI	GCTCC	5 cut(s)	70, 232, 402, 1414, 2340
Lsp1109I	GCAGC	7 cut(s)	598, 842, 1012, 1564, 1992, 2197, 2594
LweI	GCATC	6 cut(s)	116, 1018, 1437, 2546, 2566, 2595
MaeI	CTAG	5 cut(s)	350, 771, 1164, 1203, 2018
MaeII	ACGT	1 cut(s)	2878
MaeIII	GTNAC	6 cut(s)	967, 1222, 1975, 2385, 2696, 2785
MboII	GAAGA	9 cut(s)	231, 887, 1635, 1915, 1969, 1982, 2614, 2669, 2746
MflI	RGATCY	4 cut(s)	174, 249, 2662, 2767
MhlI	GDGCHC	7 cut(s)	159, 229, 408, 1465, 2166, 2323, 2337
MlyI	GAGTC	6 cut(s)	614, 650, 1432, 1562, 1877, 2517
MmeI	TCCRAC	1 cut(s)	267
Mph1103I	ATGCAT	2 cut(s)	1999, 2061
MroI	TCCGGA	1 cut(s)	620
MroXI	GAANNNNTTC	2 cut(s)	879, 1472
MslI	CAYNNNNRTG	5 cut(s)	732, 1004, 1572, 2468, 2923
MspA1I	CMGCKG	3 cut(s)	833, 2005, 2585
MspCI	CTTAAG	2 cut(s)	641, 2492
MspI	CCGG	5 cut(s)	62, 68, 178, 621, 1458
MspR9I	CCNGG	2 cut(s)	68, 2344
Mva1269I	GAATGC	3 cut(s)	1912, 2138, 2440
MvaI	CCWGG	1 cut(s)	2344
MvnI	CGCG	4 cut(s)	84, 220, 314, 2635
MwoI	GCNNNNNNNGC	9 cut(s)	224, 339, 394, 403, 434, 971, 2014, 2191, 2867
NciI	CCSGG	1 cut(s)	68
NdeI	CATATG	1 cut(s)	729
NheI	GCTAGC	1 cut(s)	1202
NlaIII	CATG	8 cut(s)	307, 586, 862, 1182, 1899, 2059, 2162, 2922
NlaIV	GGNNCC	8 cut(s)	66, 72, 228, 1541, 1701, 2154, 2664, 2769
NmeAIII	GCCGAG	4 cut(s)	112, 178, 292, 447
NmuCI	GTSAC	2 cut(s)	1975, 2385
NsiI	ATGCAT	2 cut(s)	1999, 2061
NspI	RCATGY	3 cut(s)	1182, 1899, 2059
OliI	CACNNNNGTG	1 cut(s)	1004
PagI	TCATGA	1 cut(s)	582
PceI	AGGCCT	1 cut(s)	1691
PciSI	GCTCTTC	1 cut(s)	1618
PcsI	WCGNNNNNNNCGW	2 cut(s)	132, 1543
PctI	GAATGC	3 cut(s)	1912, 2138, 2440
PdmI	GAANNNNTTC	2 cut(s)	879, 1472
PfeI	GAWTC	7 cut(s)	10, 325, 1489, 1534, 1706, 1840, 2558
PflMI	CCANNNNNTGG	1 cut(s)	384
PleI	GAGTC	6 cut(s)	613, 650, 1431, 1561, 1876, 2516
PpsI	GAGTC	6 cut(s)	613, 650, 1431, 1561, 1876, 2516
PshBI	ATTAAT	1 cut(s)	1808
PsiI	TTATAA	2 cut(s)	1212, 2898
Psp124BI	GAGCTC	2 cut(s)	408, 2166
Psp6I	CCWGG	1 cut(s)	2342
PspEI	GGTNACC	1 cut(s)	1222
PspFI	CCCAGC	4 cut(s)	1946, 2729, 2798, 2857
PspGI	CCWGG	1 cut(s)	2342
PspN4I	GGNNCC	8 cut(s)	66, 72, 228, 1541, 1701, 2154, 2664, 2769
PspPI	GGNCC	6 cut(s)	64, 274, 286, 331, 387, 724
PstI	CTGCAG	1 cut(s)	1245
PstNI	CAGNNNCTG	1 cut(s)	1709
PsuI	RGATCY	4 cut(s)	174, 249, 2662, 2767
PvuII	CAGCTG	3 cut(s)	833, 2005, 2585
RsaI	GTAC	3 cut(s)	509, 890, 1354
RsaNI	GTAC	3 cut(s)	508, 889, 1353
RseI	CAYNNNNRTG	5 cut(s)	732, 1004, 1572, 2468, 2923
SacI	GAGCTC	2 cut(s)	408, 2166
SapI	GCTCTTC	1 cut(s)	1618
Sau96I	GGNCC	6 cut(s)	64, 274, 286, 331, 387, 724
SchI	GAGTC	6 cut(s)	614, 650, 1432, 1562, 1877, 2517
ScrFI	CCNGG	2 cut(s)	68, 2344
SduI	GDGCHC	7 cut(s)	159, 229, 408, 1465, 2166, 2323, 2337
SfaNI	GCATC	6 cut(s)	116, 1018, 1437, 2546, 2566, 2595
SfcI	CTRYAG	2 cut(s)	23, 1241
SinI	GGWCC	2 cut(s)	274, 724
SmiMI	CAYNNNNRTG	5 cut(s)	732, 1004, 1572, 2468, 2923
SmlI	CTYRAG	6 cut(s)	212, 641, 939, 1451, 2276, 2492
SmoI	CTYRAG	6 cut(s)	212, 641, 939, 1451, 2276, 2492
SpeI	ACTAGT	1 cut(s)	770
SseBI	AGGCCT	1 cut(s)	1691
SspI	AATATT	3 cut(s)	761, 847, 1848
SspMI	CTAG	5 cut(s)	350, 771, 1164, 1203, 2018
SstI	GAGCTC	2 cut(s)	408, 2166
StuI	AGGCCT	1 cut(s)	1691
StyD4I	CCNGG	2 cut(s)	66, 2342
StyI	CCWWGG	2 cut(s)	418, 2170
TaaI	ACNGT	8 cut(s)	163, 461, 545, 994, 1307, 1402, 2049, 2332
TaiI	ACGT	1 cut(s)	2881
TaqI	TCGA	6 cut(s)	603, 816, 947, 1158, 1364, 2268
TaqII	GACCGA	1 cut(s)	1994
TatI	WGTACW	2 cut(s)	507, 888
TauI	GCSGC	7 cut(s)	81, 84, 188, 428, 563, 2582, 2638
TfiI	GAWTC	7 cut(s)	10, 325, 1489, 1534, 1706, 1840, 2558
TscAI	CASTG	4 cut(s)	44, 478, 1470, 2600
TseFI	GTSAC	2 cut(s)	1975, 2385
TseI	GCWGC	7 cut(s)	611, 830, 1025, 1577, 2005, 2185, 2582
Tsp45I	GTSAC	2 cut(s)	1975, 2385
TspGWI	ACGGA	1 cut(s)	181
TspRI	CASTG	4 cut(s)	44, 478, 1470, 2600
Van91I	CCANNNNNTGG	1 cut(s)	384
Vha464I	CTTAAG	2 cut(s)	641, 2492
VneI	GTGCAC	1 cut(s)	1461
VpaK11BI	GGWCC	2 cut(s)	274, 724
VspI	ATTAAT	1 cut(s)	1808
XagI	CCTNNNNNAGG	1 cut(s)	2147
XapI	RAATTY	6 cut(s)	494, 576, 694, 878, 2777, 2891
XceI	RCATGY	3 cut(s)	1182, 1899, 2059
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1287
XmnI	GAANNNNTTC	2 cut(s)	879, 1472
XspI	CTAG	5 cut(s)	350, 771, 1164, 1203, 2018
Zsp2I	ATGCAT	2 cut(s)	1999, 2061

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.