Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0011959

Physical Location & Seq

Genomic Coordinates Forward (+)

51860388 .. 51865766

Gene Structure 5379 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ58505

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2922 bp

ATGGAGTGGAACCCCCAGGACCTCCACACTCTCTCTCAGTGCTTCGTCAACACCCTCTCTCCCTCGCCGGAGCCTCGCCGCCAAGCCGAGGCCATACTCGCCGATTTCTCCCAGAAGCCCAATTACGGCCTCGCCGTGCTTCGCCTCGTCGCCGAGCCCAGCGTCGCCGAGGAGATCCGCCAGGCCGCCTCCGTCAACTTCAAGAACCACCTCAAGGCCCGGTGGGCCCCCGCTCCCAACAACTCCGACGAGCCCAGGATCCTCGACGCCGAGAAGGACCAGATCAAAGCCCTAATCGTCACCCTCATGCTCAACGCCACCCCCAAAATCCAGGGCCAGCTCAGCGAAGCCCTAGTCCTGATCGGCAAGCACGATTTCCCCAGGCTCTGGCCCACTCTGCTCCCGGAGCTCATCGGAAGCCTCCACAAGGCTTCCCAGGCCGGCAACTATGCTTCCGTTAACGGTATTCTCGGCACTGCTAACTCTATTTTTAAGAAATTTCGGTATGAGTACAAGACCAATGATCTTTTGTATGATTTGAAATACTGTTTGGATAATTTCGCCGGAACCCTATTGGAAATTTTCTTGAAAACGGCTAATTTGATTGAGTCTGCCGCCAATGCCGGCGGAGGTAATGCGGAGGCGCTCAAGCCGCTGTTTGAGTCTCAGAGGTTGTGCTGTAGGATTTTCTTCTCGTTGAATTTTCAGGAGTTGCCTGAGTTTTTCGAGGACCATATGAATGAGTGGATGACTGAGCAAAGGAAATATCTAGCCAATAGTTATCCAGCGCTTGAGAATAGTGCTGATGGGCTTGCAGTGGTGGATGAGTTGCGCTCAGCGGTGTGTGAGAATATTAACCTTTATATGGAGAAGAATGAGGAAGAGTTTCAAGCTTACTTGAATGGTTTTGCGCTAGCTGTGTGGAATTTGTTAACAACTGTCTCTCAGGCATCGAGCCGTGATCAGCTAGCCGTTACAGCTATTAAGTTCTTGACTACCGTTAGCACAAGTGTGCATCATAATCTGTTTGCTGGTGACGGAGTGATACCCCAGATTTGTCAGGGCATTGTGATCCCAAATGTGAGGTTGAGGGATGAGGATGAGGAACTGTTTGACATGAACTACATTGAGTTCATCAGGAGGGATATGGAAGGCAGTGATCTCGATACCAGGAGGAGGATTGCTTGTGAGCTTCTTAAGGGGATTGCTACCAATTATAAACCACAGGTTACCAACTTGGTTTCTGTACAGATACAGAATTTGCTGACCTCTTTTGCCGCAAACCCATCTGAGAATTGGAAGGATAAGGACTGCGCAATATACTTGGTTGTATCACTTGCTACTAAGAGGGCTGGTGGCACTTCTGTTACGACTGATCTTGTTGATGTTCAGAACTTCTTTGGGACAGTGATTGTTCCAGAACTGCAGAGTCAGGATGTGAATGGATATCCAATGCTTAAGGCGGGCGCATTGAAATTTTTCACAATGTTCCGGAATCATATACCAAAGCCTATGACATTACAATTTTTTCCAGATTTGATTCGGTTCCTTCGTGCAGAGTCCAATGTAGTTCACTCTTATGCTGCAAGTTGTATTGAGAAGCTTTTGCTGGTAAAGGATGAGGGTGGACAAGCAAGATATACTTCAGTAGATATTTCTCCAGTGCTGCCACAACTGATGACCAACCTCTTTGAGGCTTTGAAGGTTCCAGAATCTGAGGAAAATCAATATGTGATGAAGTGTATTATGCGAGTTCTCGGGGTTGCAGACATATCCCGTGAGATTGTTGGACCTTGTATTACTGGGTTGACATCAATACTCAACAAAGCTTGTGAAAACCCCAAGAATCCAGTATTTAACCACTATGTTTTTGAGTCTGTGGCTGTCCTTGTGAAACGAGCATGCGGCAAGGATGCCTCTCTCATATCGATTTTTGAGATAAACCTTTTCCCCAGTATCCAGAAGATATTGGTCGAAGATGTGCAAGAGTTCTATCCTTATGCACTTCAGCTGCTGGCTCAACTTGTTGAGTTGAACAGGCCACCCATTCCACAGAGTTACATGCAGATTTTTCCAATGCTGTTGTCTCCTGACTCGTGGAGAAAGGCTTCCAATGTCCCTGCTCTTGTGCGTTTGCTTCAAGCATTCCTTCAGAAGGCACCCCATGAGCTCAACCAAGAGGGCAGGTTGAGACAGGTCCTTGAGATTTCCAATAAGCTTGTCTCAGCCCGTAGTACTGATGAACAGGGTTTCTATGTGCTAAATACTGTTGTTGAGAGCCTTGATTTTAATGTGATTGCGCCTTACATTGGTCAGATTTGGAGTGCACTTTTCACAGTTCTCCAAAGCAGGCAGACAGGAAAGTTTATCAAGTGTCTATTGATTTTTATGTCACTCTTTCTGGTCAAACATGGCTCTACAAACCTTGTCGATTCAGTGAATAGCATTCAGGCCAACCTATTTCTGATGATCTTGGGGCAGTTTTGGATACCTAATCTTACACTTATTACAGGAGTCATTGAGACTAAGTTGACTGCAGTTGCATCAACCCGACTTCTGTGTGAATCTCAAATGCTTCTGGATGCTGCAGCCGTTGAGCACTGGGGGAAACTGCTTAACAACATTGTAACCCTTCTTTCACGAACAGAGCAGGACAGGGTTGAAGAGGATCCTGAAATGCCTGAATTTTCAGAAAATGTTGGATATTCTGCCACCTTTATTCGGCTGCACAATGCTGGGAAGACTGAGGATGATCCGCTGAAAGATATAAGAGATGCAAAGGAATTTTTGGTGACTTCGTTGGCTAGGCTTTCTGCGCTTTCCCCAGGCAGATACCCTCAAATCATCAGTCAATATGTTGAACCAACCAATCAGGCAGAACTACTTCGTCTCTGCAACTCTTATAACTGCCAAATTGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005049 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005829 GO:0005911 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0008104 GO:0008150 GO:0009506 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0017038 GO:0030054 GO:0031967 GO:0031975 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055044 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0140104 GO:0140142

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CAS_CSE1 Cse1 IBN_N

Protein Analysis

973

Amino Acids

109.26

Weight (kDa)

5.34

Isoelectric Point (pI)

42.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
IBN_N	PF03810	29 - 104	1.2e-11	Importin-beta N-terminal domain
Cse1	PF08506	163 - 532	2.3e-135	Cse1
CAS_CSE1	PF03378	533 - 961	2.6e-154	CAS/CSE protein, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011959)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46520
fragaria_vesca	FvH4_7g16830 FvH4_7g16830
malus_domestica	MD01G1083100.v1.1
prunus_persica	Prupe.2G190300_v2.0.a1 Prupe.2G190300_v2.0.a1 Prupe.2G190300_v2.0.a1 Prupe.2G190300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0360031
rosa_laevigata	RLG00000027837
rosa_multiflora	Rmu_co8506667.1_g000001 Rmu_ssc0000368.1_g000034
rosa_roxburghii	Rroxscaffold_4G00296440
rosa_rugosa	Rorug01G0274600
rosa_samantha	Rh1AG288500 Rh1BG254000 Rh1CG271500 Rh1DG283100
rosa_wichuraiana	Rw1G025580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1218, 2904
Acc16I	TGCGCA	1 cut(s)	1315
Acc36I	ACCTGC	1 cut(s)	2175
AccB1I	GGYRCC	1 cut(s)	2158
AccB7I	CCANNNNNTGG	1 cut(s)	387
AccBSI	CCGCTC	1 cut(s)	233
AccIII	TCCGGA	1 cut(s)	1491
AclWI	GGATC	7 cut(s)	169, 253, 266, 1066, 2663, 2676, 2747
AcsI	RAATTY	8 cut(s)	497, 579, 700, 925, 1258, 1475, 2684, 2783
AcuI	CTGAAG	3 cut(s)	1629, 1991, 2135
AcyI	GRCGYC	1 cut(s)	267
AfaI	GTAC	3 cut(s)	512, 1248, 2236
AfeI	AGCGCT	1 cut(s)	789
AflII	CTTAAG	2 cut(s)	1196, 1457
AjnI	CCWGG	8 cut(s)	15, 180, 254, 330, 380, 435, 1169, 2824
AjuI	GAANNNNNNNTTGG	2 cut(s)	533, 565
AleI	CACNNNNGTG	1 cut(s)	1010
Alw21I	GWGCWC	4 cut(s)	411, 2172, 2329, 2601
Alw26I	GTCTC	7 cut(s)	669, 946, 2091, 2185, 2227, 2516, 2894
Alw44I	GTGCAC	1 cut(s)	2325
AlwI	GGATC	7 cut(s)	169, 253, 266, 1066, 2663, 2676, 2747
AlwNI	CAGNNNCTG	3 cut(s)	387, 1715, 2014
Ama87I	CYCGRG	1 cut(s)	1757
Aor13HI	TCCGGA	1 cut(s)	1491
Aor51HI	AGCGCT	1 cut(s)	789
ApaI	GGGCCC	1 cut(s)	229
ApaLI	GTGCAC	1 cut(s)	2325
ApeKI	GCWGC	6 cut(s)	1583, 1666, 2011, 2585, 2588, 2725
ApoI	RAATTY	8 cut(s)	497, 579, 700, 925, 1258, 1475, 2684, 2783
ArsI	GACNNNNNNTTYG	4 cut(s)	339, 371, 2833, 2865
Asp700I	GAANNNNTTC	4 cut(s)	885, 1478, 2107, 2883
AspLEI	GCGC	8 cut(s)	646, 790, 834, 913, 1316, 1469, 2302, 2818
AsuC2I	CCSGG	2 cut(s)	220, 404
AsuHPI	GGTGA	3 cut(s)	292, 1046, 2803
AsuNHI	GCTAGC	2 cut(s)	913, 967
AvaI	CYCGRG	1 cut(s)	1757
AvaII	GGWCC	5 cut(s)	19, 277, 730, 1790, 2197
BaeGI	GKGCMC	2 cut(s)	229, 2329
BamHI	GGATCC	2 cut(s)	258, 2668
BanI	GGYRCC	1 cut(s)	2158
BanII	GRGCYC	5 cut(s)	159, 229, 255, 411, 2172
BarI	GAAGNNNNNNTAC	2 cut(s)	107, 139
BauI	CACGAG	1 cut(s)	2095
BbsI	GAAGAC	1 cut(s)	2747
Bbv12I	GWGCWC	4 cut(s)	411, 2172, 2329, 2601
BbvI	GCAGC	6 cut(s)	1570, 1653, 1998, 2572, 2600, 2712
BccI	CCATC	2 cut(s)	800, 1294
BceAI	ACGGC	6 cut(s)	119, 142, 609, 942, 956, 2576
BcgI	CGANNNNNNTGC	2 cut(s)	1144, 1178
BciT130I	CCWGG	8 cut(s)	17, 182, 256, 332, 382, 437, 1171, 2826
BciVI	GTATCC	2 cut(s)	1967, 2481
BclI	TGATCA	1 cut(s)	961
BcnI	CCSGG	2 cut(s)	220, 404
BcoDI	GTCTC	7 cut(s)	669, 946, 2091, 2185, 2227, 2516, 2894
BfaI	CTAG	5 cut(s)	353, 770, 914, 968, 2805
BfmI	CTRYAG	4 cut(s)	679, 1424, 2535, 2586
BfoI	RGCGCY	2 cut(s)	647, 791
BfrI	CTTAAG	2 cut(s)	1196, 1457
BfuAI	ACCTGC	1 cut(s)	2175
BfuI	GTATCC	2 cut(s)	1967, 2481
BglI	GCCNNNNNGGC	1 cut(s)	224
BlpI	GCTNAGC	2 cut(s)	341, 835
BmcAI	AGTACT	1 cut(s)	2236
Bme18I	GGWCC	5 cut(s)	19, 277, 730, 1790, 2197
BmeT110I	CYCGRG	1 cut(s)	1757
BmrI	ACTGGG	3 cut(s)	1812, 1947, 2611
BmsI	GCATC	6 cut(s)	959, 1024, 1903, 2552, 2572, 2764
BmtI	GCTAGC	2 cut(s)	917, 971
BmuI	ACTGGG	3 cut(s)	1812, 1947, 2611
BpiI	GAAGAC	1 cut(s)	2747
BplI	GAGNNNNNCTC	2 cut(s)	818, 850
BpmI	CTGGAG	1 cut(s)	1644
Bpu1102I	GCTNAGC	2 cut(s)	341, 835
BpuEI	CTTGAG	4 cut(s)	197, 632, 812, 2222
BpuMI	CCSGG	2 cut(s)	220, 404
Bsa29I	ATCGAT	1 cut(s)	1928
BsaHI	GRCGYC	1 cut(s)	267
BsaJI	CCNNGG	8 cut(s)	15, 87, 168, 254, 331, 380, 435, 2824
BsaWI	WCCGGW	1 cut(s)	1491
BsaXI	ACNNNNNCTCC	2 cut(s)	43, 73
Bse118I	RCCGGY	2 cut(s)	440, 623
Bse1I	ACTGG	5 cut(s)	1661, 1807, 1850, 1953, 2606
BseAI	TCCGGA	1 cut(s)	1491
BseBI	CCWGG	8 cut(s)	17, 182, 256, 332, 382, 437, 1171, 2826
BseCI	ATCGAT	1 cut(s)	1928
BseDI	CCNNGG	8 cut(s)	15, 87, 168, 254, 331, 380, 435, 2824
BseGI	GGATG	9 cut(s)	753, 829, 1099, 1105, 1441, 1624, 1918, 2587, 2755
BseNI	ACTGG	5 cut(s)	1661, 1807, 1850, 1953, 2606
BseRI	GAGGAG	2 cut(s)	185, 1189
BseSI	GKGCMC	2 cut(s)	229, 2329
BseXI	GCAGC	6 cut(s)	1570, 1653, 1998, 2572, 2600, 2712
BseYI	CCCAGC	2 cut(s)	158, 2735
BsgI	GTGCAG	2 cut(s)	1575, 2711
BshNI	GGYRCC	1 cut(s)	2158
BshVI	ATCGAT	1 cut(s)	1928
BsiHKAI	GWGCWC	4 cut(s)	411, 2172, 2329, 2601
BsiHKCI	CYCGRG	1 cut(s)	1757
BsiSI	CCGG	7 cut(s)	68, 220, 404, 441, 564, 624, 1492
BslFI	GGGAC	2 cut(s)	1417, 2102
BsmAI	GTCTC	7 cut(s)	669, 946, 2091, 2185, 2227, 2516, 2894
BsmBI	CGTCTC	1 cut(s)	2894
BsmFI	GGGAC	2 cut(s)	1417, 2102
BsmI	GAATGC	2 cut(s)	2144, 2445
BsoBI	CYCGRG	1 cut(s)	1757
Bsp120I	GGGCCC	1 cut(s)	225
Bsp1286I	GDGCHC	7 cut(s)	159, 229, 255, 411, 2172, 2329, 2601
Bsp13I	TCCGGA	1 cut(s)	1491
Bsp1407I	TGTACA	1 cut(s)	1246
Bsp1720I	GCTNAGC	2 cut(s)	341, 835
BspDI	ATCGAT	1 cut(s)	1928
BspEI	TCCGGA	1 cut(s)	1491
BspMAI	CTGCAG	3 cut(s)	1428, 2539, 2590
BspMI	ACCTGC	1 cut(s)	2175
BspOI	GCTAGC	2 cut(s)	917, 971
BspPI	GGATC	7 cut(s)	169, 253, 266, 1066, 2663, 2676, 2747
BspT107I	GGYRCC	1 cut(s)	2158
BspTI	CTTAAG	2 cut(s)	1196, 1457
BsrBI	CCGCTC	1 cut(s)	233
BsrFI	RCCGGY	2 cut(s)	440, 623
BsrGI	TGTACA	1 cut(s)	1246
BsrI	ACTGG	5 cut(s)	1661, 1807, 1850, 1953, 2606
BssAI	RCCGGY	2 cut(s)	440, 623
BssECI	CCNNGG	8 cut(s)	15, 87, 168, 254, 331, 380, 435, 2824
BssNI	GRCGYC	1 cut(s)	267
BssSI	CACGAG	1 cut(s)	2095
Bst2BI	CACGAG	1 cut(s)	2095
Bst2UI	CCWGG	8 cut(s)	17, 182, 256, 332, 382, 437, 1171, 2826
Bst4CI	ACNGT	8 cut(s)	464, 548, 940, 1000, 1110, 1408, 2269, 2338
Bst6I	CTCTTC	2 cut(s)	876, 2658
BstACI	GRCGYC	1 cut(s)	267
BstAFI	CTTAAG	2 cut(s)	1196, 1457
BstAUI	TGTACA	1 cut(s)	1246
BstEII	GGTNACC	1 cut(s)	1228
BstENI	CCTNNNNNAGG	3 cut(s)	425, 1691, 2153
BstF5I	GGATG	9 cut(s)	753, 829, 1099, 1105, 1441, 1624, 1918, 2587, 2755
BstH2I	RGCGCY	2 cut(s)	647, 791
BstHHI	GCGC	8 cut(s)	646, 790, 834, 913, 1316, 1469, 2302, 2818
BstMAI	GTCTC	7 cut(s)	669, 946, 2091, 2185, 2227, 2516, 2894
BstNI	CCWGG	8 cut(s)	17, 182, 256, 332, 382, 437, 1171, 2826
BstNSI	RCATGY	2 cut(s)	1905, 2065
BstPI	GGTNACC	1 cut(s)	1228
BstSFI	CTRYAG	4 cut(s)	679, 1424, 2535, 2586
BstSLI	GKGCMC	2 cut(s)	229, 2329
BstV1I	GCAGC	6 cut(s)	1570, 1653, 1998, 2572, 2600, 2712
BstV2I	GAAGAC	1 cut(s)	2747
BstX2I	RGATCY	3 cut(s)	174, 258, 2668
BstYI	RGATCY	3 cut(s)	174, 258, 2668
Bsu15I	ATCGAT	1 cut(s)	1928
BsuI	GTATCC	2 cut(s)	1967, 2481
BsuTUI	ATCGAT	1 cut(s)	1928
BtsCI	GGATG	9 cut(s)	753, 829, 1099, 1105, 1441, 1624, 1918, 2587, 2755
BtsI	GCAGTG	3 cut(s)	474, 822, 1162
BtsIMutI	CAGTG	8 cut(s)	44, 474, 822, 1162, 1413, 1668, 2442, 2599
BveI	ACCTGC	1 cut(s)	2175
CaiI	CAGNNNCTG	3 cut(s)	387, 1715, 2014
CfoI	GCGC	8 cut(s)	646, 790, 834, 913, 1316, 1469, 2302, 2818
Cfr10I	RCCGGY	2 cut(s)	440, 623
ClaI	ATCGAT	1 cut(s)	1928
CseI	GACGC	2 cut(s)	151, 275
Csp6I	GTAC	3 cut(s)	511, 1247, 2235
CviAII	CATG	6 cut(s)	307, 1117, 1902, 2062, 2165, 2411
CviQI	GTAC	3 cut(s)	511, 1247, 2235
Eam1104I	CTCTTC	2 cut(s)	876, 2658
EarI	CTCTTC	2 cut(s)	876, 2658
EciI	GGCGGA	2 cut(s)	167, 642
Ecl136II	GAGCTC	2 cut(s)	409, 2170
Eco24I	GRGCYC	5 cut(s)	159, 229, 255, 411, 2172
Eco32I	GATATC	1 cut(s)	1448
Eco47I	GGWCC	5 cut(s)	19, 277, 730, 1790, 2197
Eco47III	AGCGCT	1 cut(s)	789
Eco53kI	GAGCTC	2 cut(s)	409, 2170
Eco57I	CTGAAG	3 cut(s)	1629, 1991, 2135
Eco88I	CYCGRG	1 cut(s)	1757
Eco91I	GGTNACC	1 cut(s)	1228
EcoICRI	GAGCTC	2 cut(s)	409, 2170
EcoNI	CCTNNNNNAGG	3 cut(s)	425, 1691, 2153
EcoO109I	RGGNCCY	3 cut(s)	19, 226, 2197
EcoO65I	GGTNACC	1 cut(s)	1228
EcoRII	CCWGG	8 cut(s)	15, 180, 254, 330, 380, 435, 1169, 2824
EcoRV	GATATC	1 cut(s)	1448
EcoT38I	GRGCYC	5 cut(s)	159, 229, 255, 411, 2172
Esp3I	CGTCTC	1 cut(s)	2894
FaeI	CATG	6 cut(s)	310, 1120, 1905, 2065, 2168, 2414
FalI	AAGNNNNNCTT	4 cut(s)	1627, 1659, 2133, 2165
FaqI	GGGAC	2 cut(s)	1417, 2102
FatI	CATG	6 cut(s)	306, 1116, 1901, 2061, 2164, 2410
FauI	CCCGC	2 cut(s)	238, 1456
FauNDI	CATATG	1 cut(s)	735
FbaI	TGATCA	1 cut(s)	961
FokI	GGATG	9 cut(s)	760, 836, 1106, 1112, 1448, 1631, 1925, 2594, 2762
FriOI	GRGCYC	5 cut(s)	159, 229, 255, 411, 2172
FspBI	CTAG	5 cut(s)	353, 770, 914, 968, 2805
FspI	TGCGCA	1 cut(s)	1315
GlaI	GCGC	8 cut(s)	645, 789, 833, 912, 1315, 1468, 2301, 2817
GsaI	CCCAGC	2 cut(s)	162, 2739
GsuI	CTGGAG	1 cut(s)	1644
HaeII	RGCGCY	2 cut(s)	647, 791
HapII	CCGG	7 cut(s)	68, 220, 404, 441, 564, 624, 1492
HgaI	GACGC	2 cut(s)	151, 275
HhaI	GCGC	8 cut(s)	646, 790, 834, 913, 1316, 1469, 2302, 2818
Hin1I	GRCGYC	1 cut(s)	267
Hin1II	CATG	6 cut(s)	310, 1120, 1905, 2065, 2168, 2414
Hin6I	GCGC	8 cut(s)	644, 788, 832, 911, 1314, 1467, 2300, 2816
HinP1I	GCGC	8 cut(s)	644, 788, 832, 911, 1314, 1467, 2300, 2816
HincII	GTYRAC	6 cut(s)	49, 196, 460, 933, 1809, 2532
HindII	GTYRAC	6 cut(s)	49, 196, 460, 933, 1809, 2532
HindIII	AAGCTT	4 cut(s)	891, 1601, 1827, 2216
HpaI	GTTAAC	2 cut(s)	460, 933
HpaII	CCGG	7 cut(s)	68, 220, 404, 441, 564, 624, 1492
HphI	GGTGA	3 cut(s)	292, 1046, 2803
Hpy166II	GTNNAC	9 cut(s)	49, 196, 460, 933, 1573, 1628, 1809, 2327, 2532
Hpy8I	GTNNAC	9 cut(s)	49, 196, 460, 933, 1573, 1628, 1809, 2327, 2532
Hpy99I	CGWCG	4 cut(s)	152, 167, 251, 269
HpyAV	CCTTC	8 cut(s)	268, 1145, 1294, 1559, 1696, 2149, 2159, 2642
HpyCH4III	ACNGT	8 cut(s)	464, 548, 940, 1000, 1110, 1408, 2269, 2338
Hsp92I	GRCGYC	1 cut(s)	267
Hsp92II	CATG	6 cut(s)	310, 1120, 1905, 2065, 2168, 2414
HspAI	GCGC	8 cut(s)	644, 788, 832, 911, 1314, 1467, 2300, 2816
Kpn2I	TCCGGA	1 cut(s)	1491
KroI	GCCGGC	2 cut(s)	440, 623
KroNI	GCCGGC	2 cut(s)	442, 625
Ksp22I	TGATCA	1 cut(s)	961
KspAI	GTTAAC	2 cut(s)	460, 933
LmnI	GCTCC	4 cut(s)	70, 238, 405, 406
Lsp1109I	GCAGC	6 cut(s)	1570, 1653, 1998, 2572, 2600, 2712
LweI	GCATC	6 cut(s)	959, 1024, 1903, 2552, 2572, 2764
MaeI	CTAG	5 cut(s)	353, 770, 914, 968, 2805
MaeIII	GTNAC	9 cut(s)	298, 973, 1034, 1228, 1366, 2057, 2391, 2626, 2791
MbiI	CCGCTC	1 cut(s)	233
MboII	GAAGA	7 cut(s)	682, 883, 893, 1975, 1988, 2675, 2752
MflI	RGATCY	3 cut(s)	174, 258, 2668
MhlI	GDGCHC	7 cut(s)	159, 229, 255, 411, 2172, 2329, 2601
MlyI	GAGTC	7 cut(s)	617, 671, 1438, 1568, 1883, 2087, 2523
MmeI	TCCRAC	3 cut(s)	270, 1768, 2680
MroI	TCCGGA	1 cut(s)	1491
MroNI	GCCGGC	2 cut(s)	440, 623
MroXI	GAANNNNTTC	4 cut(s)	885, 1478, 2107, 2883
MslI	CAYNNNNRTG	4 cut(s)	305, 738, 1010, 1578
MspA1I	CMGCKG	4 cut(s)	655, 839, 2011, 2758
MspCI	CTTAAG	2 cut(s)	1196, 1457
MspI	CCGG	7 cut(s)	68, 220, 404, 441, 564, 624, 1492
Mva1269I	GAATGC	2 cut(s)	2144, 2445
MvaI	CCWGG	8 cut(s)	17, 182, 256, 332, 382, 437, 1171, 2826
NaeI	GCCGGC	2 cut(s)	442, 625
NciI	CCSGG	2 cut(s)	220, 404
NdeI	CATATG	1 cut(s)	735
NgoMIV	GCCGGC	2 cut(s)	440, 623
NheI	GCTAGC	2 cut(s)	913, 967
NlaIII	CATG	6 cut(s)	310, 1120, 1905, 2065, 2168, 2414
NmeAIII	GCCGAG	5 cut(s)	112, 178, 193, 295, 450
NmuCI	GTSAC	4 cut(s)	298, 1034, 2391, 2791
NsbI	TGCGCA	1 cut(s)	1315
NspI	RCATGY	2 cut(s)	1905, 2065
OliI	CACNNNNGTG	1 cut(s)	1010
PaeI	GCATGC	1 cut(s)	1905
PcsI	WCGNNNNNNNCGW	3 cut(s)	132, 369, 1549
PctI	GAATGC	2 cut(s)	2144, 2445
PdiI	GCCGGC	2 cut(s)	442, 625
PdmI	GAANNNNTTC	4 cut(s)	885, 1478, 2107, 2883
PfeI	GAWTC	6 cut(s)	1495, 1540, 1712, 1846, 2432, 2564
PflMI	CCANNNNNTGG	1 cut(s)	387
PfoI	TCCNGGA	1 cut(s)	402
PleI	GAGTC	7 cut(s)	616, 670, 1437, 1567, 1882, 2087, 2522
PpsI	GAGTC	7 cut(s)	616, 670, 1437, 1567, 1882, 2087, 2522
PpuMI	RGGWCCY	2 cut(s)	19, 2197
PsiI	TTATAA	2 cut(s)	1218, 2904
Psp124BI	GAGCTC	2 cut(s)	411, 2172
Psp5II	RGGWCCY	2 cut(s)	19, 2197
Psp6I	CCWGG	8 cut(s)	15, 180, 254, 330, 380, 435, 1169, 2824
PspEI	GGTNACC	1 cut(s)	1228
PspFI	CCCAGC	2 cut(s)	158, 2735
PspGI	CCWGG	8 cut(s)	15, 180, 254, 330, 380, 435, 1169, 2824
PspOMI	GGGCCC	1 cut(s)	225
PspPPI	RGGWCCY	2 cut(s)	19, 2197
PstI	CTGCAG	3 cut(s)	1428, 2539, 2590
PstNI	CAGNNNCTG	3 cut(s)	387, 1715, 2014
PsuI	RGATCY	3 cut(s)	174, 258, 2668
PvuII	CAGCTG	1 cut(s)	2011
RsaI	GTAC	3 cut(s)	512, 1248, 2236
RsaNI	GTAC	3 cut(s)	511, 1247, 2235
RseI	CAYNNNNRTG	4 cut(s)	305, 738, 1010, 1578
SacI	GAGCTC	2 cut(s)	411, 2172
ScaI	AGTACT	1 cut(s)	2236
SchI	GAGTC	7 cut(s)	617, 671, 1438, 1568, 1883, 2087, 2523
SduI	GDGCHC	7 cut(s)	159, 229, 255, 411, 2172, 2329, 2601
SfaNI	GCATC	6 cut(s)	959, 1024, 1903, 2552, 2572, 2764
SfcI	CTRYAG	4 cut(s)	679, 1424, 2535, 2586
SfiI	GGCCNNNNNGGCC	1 cut(s)	224
SinI	GGWCC	5 cut(s)	19, 277, 730, 1790, 2197
SmiMI	CAYNNNNRTG	4 cut(s)	305, 738, 1010, 1578
SmlI	CTYRAG	6 cut(s)	212, 647, 791, 1196, 1457, 2201
SmoI	CTYRAG	6 cut(s)	212, 647, 791, 1196, 1457, 2201
SphI	GCATGC	1 cut(s)	1905
SspI	AATATT	1 cut(s)	853
SspMI	CTAG	5 cut(s)	353, 770, 914, 968, 2805
SstI	GAGCTC	2 cut(s)	411, 2172
TaaI	ACNGT	8 cut(s)	464, 548, 940, 1000, 1110, 1408, 2269, 2338
TaqI	TCGA	7 cut(s)	264, 726, 953, 1164, 1928, 1974, 2430
TatI	WGTACW	3 cut(s)	510, 1246, 2234
TauI	GCSGC	6 cut(s)	81, 188, 617, 655, 1280, 1908
TfiI	GAWTC	6 cut(s)	1495, 1540, 1712, 1846, 2432, 2564
TscAI	CASTG	8 cut(s)	44, 481, 822, 1162, 1413, 1668, 2442, 2606
TseFI	GTSAC	4 cut(s)	298, 1034, 2391, 2791
TseI	GCWGC	6 cut(s)	1583, 1666, 2011, 2585, 2588, 2725
Tsp45I	GTSAC	4 cut(s)	298, 1034, 2391, 2791
TspDTI	ATGAA	5 cut(s)	752, 1123, 1133, 1751, 2256
TspGWI	ACGGA	3 cut(s)	181, 445, 1053
TspRI	CASTG	8 cut(s)	44, 481, 822, 1162, 1413, 1668, 2442, 2606
Van91I	CCANNNNNTGG	1 cut(s)	387
Vha464I	CTTAAG	2 cut(s)	1196, 1457
VneI	GTGCAC	1 cut(s)	2325
VpaK11BI	GGWCC	5 cut(s)	19, 277, 730, 1790, 2197
XagI	CCTNNNNNAGG	3 cut(s)	425, 1691, 2153
XapI	RAATTY	8 cut(s)	497, 579, 700, 925, 1258, 1475, 2684, 2783
XceI	RCATGY	2 cut(s)	1905, 2065
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1293
XmnI	GAANNNNTTC	4 cut(s)	885, 1478, 2107, 2883
XspI	CTAG	5 cut(s)	353, 770, 914, 968, 2805
ZrmI	AGTACT	1 cut(s)	2236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.