Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012710

Physical Location & Seq

Genomic Coordinates Reverse (-)

19498360 .. 19504648

Gene Structure 6289 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1086500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGGGCCAGTGCTACGGCAAAACCATTCCCACCACCGAAAATGACCCCAACACCACCACCGTAACTACAATCACTACCTCCGTTCACCAACGTCAAACGACGCCGTCTCAGGCCGGGCAGAACGGGGCCGTCAGCGTAAAAACCACGCCGGCCAGGTCGTCGAACCCTAGCCCGTGGCCGAGCCCCTACCCACACGGTGTATCGGCCAGCCCGTTGCCCGATGGAGTGTCGCCGTCTCCGGCGAGGTCGTCGACTCCGGGTAGGTTTTTCAGGCGGAGGTTCGCGCCTCCGTCTCCGGCTAAGCATATAAAGGCGTCGCTAGCGAAGCGTTTTGGGAAGCCGAAGGAGAGTCCGATTCCGGAGGAGGGTGGGGCGGAGCCGGAGCAGTCGCTGGACAAGAGTTTCGGGTACGGGAAGAATTTCGGAGCTAAGTACGAGCTCGGGAAGGAGGTGGGGCGAGGGCATTTTGGTCATACTTGCTCTGGTAGGGGAAAGAAGGGTGAGCACAAGGACCAGGCTGTGGCTGTTAAGATAATTTCGAAAGCCAAGATGACGACAGCTATATCAATTGAAGATGTTCGTAGAGAGGTGAAGATATTGAAAGCTCTATCTGGGCATAAGCATCTTGTAAAATTTTATGATGCATGTGAAGACGCCAATAATGTTTACATTGTCATGGAATTGTGTGAAGGGGGGGAACTTCTTGACAGAATTTTATCTAGAGGCGGAAAGTATGCAGAGGAAGATGCAAAACACATAGTTGTGCAAATCTTAAGTGTTGTTTCATTTTGTCATCTTCAAGGCGTTGTGCACCGTGACTTAAAACCAGAGAATTTCCTTTTCGCTTCTAGAAGTGAAGATGCCGATATGAAGCTTATTGATTTTGGTCTTTCTGACTTTATCAGACCAGATGAAAGACTTAATGACATTGTTGGAAGTGCATACTATGTTGCGCCTGAAGTCCTCCATAGATCTTATAGCCTTGAGGGAGATATATGGAGCATTGGTGTTATCACCTATATCCTGCTATGTGGAAGCCGACCTTTCTGGGCACGAACCGAATCTGGAATTTTCCGTGCAGTGCTAAGAGCTGATCCTAGCTTCGATGATTTACCCTGGCCTACTATATCTCTAGAGGCCAAGGACTTCGTAAGAAGACTTTTGAATAAGGACTACAGAAAAAGAATGACTGCTGTCCAAGCCTTGTCTCACCCATGGTTGCGAGATGACAGTCGTCCTCTTCCTCTAGATATATTGATATATAAATTAGTCAAATCATATCTCCATGCTACGCCGTTCAAACGTGCAGCACTTAAGTCTCTTTCCAAAGCTTTGACGGAGGAAGAGCTGATATATCTAAGAGCCCAATTCATGCTATTGGAACCAGATAAAGATGAGCGTGTTTCTCTTCGTAATTTCAAAATGGCTCTACAGAGAAATTCAACTGAAGCGATGAAAGAGTCAAAGGTTGTTGATATCCCAAATGCGATGGAACCGCTTGCTTATAGAACAATGAACTTCGAAGAATTTTGTGCAGCCGCAATCAGTACCCATCAATTGGAGGCCCTTGAAGGGTGGGAGCAGATAGCCCGTACCGCTTTTGAGCATTTTGAACAGGAGGGTAACCGAGTCATATCAATTGAAGAACTGGCAAGAGAATTAAATCTCGGCCCTTCTGCCTACAATATCCTCCGAGATTGGATCAGAAGCGCAGACGGAAAGCTCAATTTCCTTGGATATACAAAATTTTTGCACGGTGTGACGATCCGGAGCTCCAACACCAGACACCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010150 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

Pkinase

Protein Analysis

598

Amino Acids

66.94

Weight (kDa)

8.88

Isoelectric Point (pI)

48.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	145 - 407	1.6e-70	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	146 - 361	1.2e-34	Protein tyrosine and serine/threonine kinase
Kdo	PF06293	192 - 298	3.1e-06	Lipopolysaccharide kinase (Kdo/WaaP) family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012710)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46700 AT2G46700 AT2G46700
fragaria_vesca	FvH4_7g17350 FvH4_7g17350
malus_domestica	MD01G1086500.v1.1 MD07G1156500.v1.1
prunus_persica	Prupe.2G194900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0360791
rosa_laevigata	RLG00000027786
rosa_multiflora	Rmu_sc0000692.1_g000010
rosa_roxburghii	Rroxscaffold_4G00295810
rosa_rugosa	Rorug01G0281500
rosa_samantha	Rh1AG293500 Rh1BG258100 Rh1CG276000 Rh1DG288400
rosa_wichuraiana	Rw1G025970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	520, 1558
AccI	GTMKAC	1 cut(s)	251
AccII	CGCG	1 cut(s)	284
AccIII	TCCGGA	2 cut(s)	358, 1767
AciI	CCGC	6 cut(s)	274, 374, 726, 1496, 1539, 1596
AclWI	GGATC	3 cut(s)	1088, 1709, 1759
AcoI	YGGCCR	3 cut(s)	150, 176, 204
AcsI	RAATTY	8 cut(s)	418, 632, 711, 832, 1068, 1438, 1526, 1745
AcuI	CTGAAG	2 cut(s)	978, 1467
AcyI	GRCGYC	3 cut(s)	101, 314, 654
AdeI	CACNNNGTG	2 cut(s)	197, 1759
AfaI	GTAC	4 cut(s)	410, 434, 1549, 1594
AfiI	CCNNNNNNNGG	4 cut(s)	365, 520, 1558, 1572
AflII	CTTAAG	2 cut(s)	772, 1313
AjnI	CCWGG	3 cut(s)	152, 513, 1115
Alw21I	GWGCWC	4 cut(s)	441, 507, 813, 1775
Alw26I	GTCTC	5 cut(s)	111, 240, 297, 1212, 1323
Alw44I	GTGCAC	1 cut(s)	809
AlwI	GGATC	3 cut(s)	1088, 1709, 1759
AlwNI	CAGNNNCTG	1 cut(s)	391
Ama87I	CYCGRG	1 cut(s)	440
Aor13HI	TCCGGA	2 cut(s)	358, 1767
ApaLI	GTGCAC	1 cut(s)	809
ApeKI	GCWGC	2 cut(s)	1307, 1535
ApoI	RAATTY	8 cut(s)	418, 632, 711, 832, 1068, 1438, 1526, 1745
ArsI	GACNNNNNNTTYG	2 cut(s)	386, 418
Asp700I	GAANNNNTTC	2 cut(s)	419, 576
AspLEI	GCGC	3 cut(s)	286, 955, 1712
AspS9I	GGNCC	5 cut(s)	4, 126, 511, 1564, 1670
AsuC2I	CCSGG	2 cut(s)	115, 258
AsuHPI	GGTGA	5 cut(s)	77, 512, 601, 1006, 1202
AsuII	TTCGAA	2 cut(s)	539, 1521
AsuNHI	GCTAGC	1 cut(s)	319
AvaI	CYCGRG	1 cut(s)	440
AvaII	GGWCC	1 cut(s)	511
BaeGI	GKGCMC	2 cut(s)	813, 1054
BanII	GRGCYC	4 cut(s)	185, 441, 1366, 1775
BbsI	GAAGAC	2 cut(s)	657, 1162
Bbv12I	GWGCWC	4 cut(s)	441, 507, 813, 1775
BbvI	GCAGC	2 cut(s)	1319, 1547
BccI	CCATC	3 cut(s)	215, 1483, 1560
BceAI	ACGGC	5 cut(s)	31, 88, 113, 217, 1279
BciT130I	CCWGG	3 cut(s)	154, 515, 1117
BcnI	CCSGG	2 cut(s)	115, 258
BcoDI	GTCTC	5 cut(s)	111, 240, 297, 1212, 1323
BfaI	CTAG	7 cut(s)	168, 320, 720, 849, 1098, 1133, 1247
BfmI	CTRYAG	2 cut(s)	1174, 1430
BfrI	CTTAAG	2 cut(s)	772, 1313
BglII	AGATCT	1 cut(s)	971
BisI	GCNGC	3 cut(s)	1308, 1536, 1539
BlpI	GCTNAGC	1 cut(s)	300
BlsI	GCNGC	3 cut(s)	1309, 1537, 1540
Bme1390I	CCNGG	5 cut(s)	115, 154, 258, 515, 1117
Bme18I	GGWCC	1 cut(s)	511
BmeT110I	CYCGRG	1 cut(s)	440
BmgT120I	GGNCC	5 cut(s)	4, 126, 511, 1564, 1670
BmiI	GGNNCC	4 cut(s)	127, 378, 1383, 1494
BmrFI	CCNGG	5 cut(s)	115, 154, 258, 515, 1117
BmsI	GCATC	4 cut(s)	631, 631, 736, 850
BmtI	GCTAGC	1 cut(s)	323
BoxI	GACNNNNGTC	2 cut(s)	1193, 1233
BpiI	GAAGAC	2 cut(s)	657, 1162
Bpu1102I	GCTNAGC	1 cut(s)	300
Bpu14I	TTCGAA	2 cut(s)	539, 1521
BpuEI	CTTGAG	1 cut(s)	1004
BpuMI	CCSGG	2 cut(s)	115, 258
BsaHI	GRCGYC	3 cut(s)	101, 314, 654
BsaJI	CCNNGG	5 cut(s)	173, 1115, 1140, 1214, 1732
BsaWI	WCCGGW	2 cut(s)	358, 1767
BsaXI	ACNNNNNCTCC	2 cut(s)	991, 1021
Bsc4I	CCNNNNNNNGG	4 cut(s)	365, 520, 1558, 1572
Bse118I	RCCGGY	1 cut(s)	148
Bse1I	ACTGG	2 cut(s)	7, 1653
BseAI	TCCGGA	2 cut(s)	358, 1767
BseBI	CCWGG	3 cut(s)	154, 515, 1117
BseDI	CCNNGG	5 cut(s)	173, 1115, 1140, 1214, 1732
BseLI	CCNNNNNNNGG	4 cut(s)	365, 520, 1558, 1572
BseMII	CTCAG	1 cut(s)	122
BseNI	ACTGG	2 cut(s)	7, 1653
BseRI	GAGGAG	1 cut(s)	377
BseSI	GKGCMC	2 cut(s)	813, 1054
BseXI	GCAGC	2 cut(s)	1319, 1547
BsgI	GTGCAG	3 cut(s)	1098, 1326, 1554
Bsh1236I	CGCG	1 cut(s)	284
BsiHKAI	GWGCWC	4 cut(s)	441, 507, 813, 1775
BsiHKCI	CYCGRG	1 cut(s)	440
BsiSI	CCGG	8 cut(s)	114, 149, 239, 257, 296, 359, 380, 1768
BslI	CCNNNNNNNGG	4 cut(s)	365, 520, 1558, 1572
BsmAI	GTCTC	5 cut(s)	111, 240, 297, 1212, 1323
BsmBI	CGTCTC	3 cut(s)	111, 240, 297
BsoBI	CYCGRG	1 cut(s)	440
Bsp119I	TTCGAA	2 cut(s)	539, 1521
Bsp1286I	GDGCHC	7 cut(s)	185, 441, 507, 813, 1054, 1366, 1775
Bsp13I	TCCGGA	2 cut(s)	358, 1767
Bsp143I	GATC	4 cut(s)	971, 1093, 1701, 1764
Bsp1720I	GCTNAGC	1 cut(s)	300
Bsp19I	CCATGG	1 cut(s)	1214
BspACI	CCGC	6 cut(s)	274, 374, 726, 1496, 1539, 1596
BspCNI	CTCAG	1 cut(s)	121
BspEI	TCCGGA	2 cut(s)	358, 1767
BspFNI	CGCG	1 cut(s)	284
BspLI	GGNNCC	4 cut(s)	127, 378, 1383, 1494
BspOI	GCTAGC	1 cut(s)	323
BspPI	GGATC	3 cut(s)	1088, 1709, 1759
BspQI	GCTCTTC	1 cut(s)	1338
BspT104I	TTCGAA	2 cut(s)	539, 1521
BspTI	CTTAAG	2 cut(s)	772, 1313
BsrFI	RCCGGY	1 cut(s)	148
BsrI	ACTGG	2 cut(s)	7, 1653
BssAI	RCCGGY	1 cut(s)	148
BssECI	CCNNGG	5 cut(s)	173, 1115, 1140, 1214, 1732
BssMI	GATC	4 cut(s)	971, 1093, 1701, 1764
BssNI	GRCGYC	3 cut(s)	101, 314, 654
BssT1I	CCWWGG	3 cut(s)	1140, 1214, 1732
Bst2UI	CCWGG	3 cut(s)	154, 515, 1117
Bst4CI	ACNGT	5 cut(s)	61, 197, 815, 1232, 1757
Bst6I	CTCTTC	3 cut(s)	1245, 1338, 1413
BstACI	GRCGYC	3 cut(s)	101, 314, 654
BstAFI	CTTAAG	2 cut(s)	772, 1313
BstBI	TTCGAA	2 cut(s)	539, 1521
BstC8I	GCNNGC	4 cut(s)	150, 208, 321, 1500
BstDEI	CTNAG	5 cut(s)	108, 300, 429, 1085, 1358
BstDSI	CCRYGG	2 cut(s)	173, 1214
BstEII	GGTNACC	1 cut(s)	1622
BstFNI	CGCG	1 cut(s)	284
BstHHI	GCGC	3 cut(s)	286, 955, 1712
BstKTI	GATC	4 cut(s)	974, 1096, 1704, 1767
BstMAI	GTCTC	5 cut(s)	111, 240, 297, 1212, 1323
BstMBI	GATC	4 cut(s)	971, 1093, 1701, 1764
BstMWI	GCNNNNNNNGC	4 cut(s)	320, 325, 1199, 1595
BstNI	CCWGG	3 cut(s)	154, 515, 1117
BstNSI	RCATGY	1 cut(s)	648
BstPAI	GACNNNNGTC	2 cut(s)	1193, 1233
BstPI	GGTNACC	1 cut(s)	1622
BstSCI	CCNGG	5 cut(s)	113, 152, 256, 513, 1115
BstSFI	CTRYAG	2 cut(s)	1174, 1430
BstSLI	GKGCMC	2 cut(s)	813, 1054
BstUI	CGCG	1 cut(s)	284
BstV1I	GCAGC	2 cut(s)	1319, 1547
BstV2I	GAAGAC	2 cut(s)	657, 1162
BstX2I	RGATCY	1 cut(s)	971
BstYI	RGATCY	1 cut(s)	971
BtgI	CCRYGG	2 cut(s)	173, 1214
BtgZI	GCGATG	2 cut(s)	1466, 1502
BtsI	GCAGTG	1 cut(s)	1086
BtsIMutI	CAGTG	2 cut(s)	14, 1086
Cac8I	GCNNGC	4 cut(s)	150, 208, 321, 1500
CaiI	CAGNNNCTG	1 cut(s)	391
CfoI	GCGC	3 cut(s)	286, 955, 1712
Cfr10I	RCCGGY	1 cut(s)	148
Cfr13I	GGNCC	5 cut(s)	4, 126, 511, 1564, 1670
CseI	GACGC	3 cut(s)	109, 303, 662
Csp6I	GTAC	4 cut(s)	409, 433, 1548, 1593
CviAII	CATG	5 cut(s)	645, 676, 1215, 1286, 1372
CviQI	GTAC	4 cut(s)	409, 433, 1548, 1593
DdeI	CTNAG	5 cut(s)	108, 300, 429, 1085, 1358
DpnI	GATC	4 cut(s)	973, 1095, 1703, 1766
DpnII	GATC	4 cut(s)	971, 1093, 1701, 1764
DraIII	CACNNNGTG	2 cut(s)	197, 1759
EaeI	YGGCCR	3 cut(s)	150, 176, 204
Eam1104I	CTCTTC	3 cut(s)	1245, 1338, 1413
EarI	CTCTTC	3 cut(s)	1245, 1338, 1413
EciI	GGCGGA	3 cut(s)	289, 389, 741
Ecl136II	GAGCTC	2 cut(s)	439, 1773
Eco130I	CCWWGG	3 cut(s)	1140, 1214, 1732
Eco24I	GRGCYC	4 cut(s)	185, 441, 1366, 1775
Eco32I	GATATC	1 cut(s)	1477
Eco47I	GGWCC	1 cut(s)	511
Eco53kI	GAGCTC	2 cut(s)	439, 1773
Eco57I	CTGAAG	2 cut(s)	978, 1467
Eco88I	CYCGRG	1 cut(s)	440
Eco91I	GGTNACC	1 cut(s)	1622
EcoICRI	GAGCTC	2 cut(s)	439, 1773
EcoO109I	RGGNCCY	1 cut(s)	1564
EcoO65I	GGTNACC	1 cut(s)	1622
EcoRII	CCWGG	3 cut(s)	152, 513, 1115
EcoRV	GATATC	1 cut(s)	1477
EcoT14I	CCWWGG	3 cut(s)	1140, 1214, 1732
EcoT22I	ATGCAT	1 cut(s)	646
EcoT38I	GRGCYC	4 cut(s)	185, 441, 1366, 1775
ErhI	CCWWGG	3 cut(s)	1140, 1214, 1732
Esp3I	CGTCTC	3 cut(s)	111, 240, 297
FaeI	CATG	5 cut(s)	648, 679, 1218, 1289, 1375
FalI	AAGNNNNNCTT	2 cut(s)	1027, 1059
FatI	CATG	5 cut(s)	644, 675, 1214, 1285, 1371
FblI	GTMKAC	1 cut(s)	251
Fnu4HI	GCNGC	3 cut(s)	1308, 1536, 1539
FriOI	GRGCYC	4 cut(s)	185, 441, 1366, 1775
Fsp4HI	GCNGC	3 cut(s)	1308, 1536, 1539
FspBI	CTAG	7 cut(s)	168, 320, 720, 849, 1098, 1133, 1247
GlaI	GCGC	3 cut(s)	285, 954, 1711
GluI	GCNGC	3 cut(s)	1308, 1536, 1539
HapII	CCGG	8 cut(s)	114, 149, 239, 257, 296, 359, 380, 1768
HgaI	GACGC	3 cut(s)	109, 303, 662
HhaI	GCGC	3 cut(s)	286, 955, 1712
Hin1I	GRCGYC	3 cut(s)	101, 314, 654
Hin1II	CATG	5 cut(s)	648, 679, 1218, 1289, 1375
Hin6I	GCGC	3 cut(s)	284, 953, 1710
HinP1I	GCGC	3 cut(s)	284, 953, 1710
HincII	GTYRAC	1 cut(s)	252
HindII	GTYRAC	1 cut(s)	252
HindIII	AAGCTT	2 cut(s)	872, 1329
HinfI	GANTC	6 cut(s)	253, 349, 355, 1061, 1460, 1629
HpaII	CCGG	8 cut(s)	114, 149, 239, 257, 296, 359, 380, 1768
HphI	GGTGA	5 cut(s)	77, 512, 601, 1006, 1202
Hpy166II	GTNNAC	4 cut(s)	85, 252, 667, 811
Hpy188I	TCNGA	6 cut(s)	354, 425, 895, 905, 1694, 1706
Hpy188III	TCNNGA	9 cut(s)	359, 442, 704, 720, 849, 1065, 1133, 1247, 1768
Hpy8I	GTNNAC	4 cut(s)	85, 252, 667, 811
Hpy99I	CGWCG	4 cut(s)	103, 163, 253, 319
HpyAV	CCTTC	6 cut(s)	337, 439, 490, 683, 1565, 1683
HpyCH4III	ACNGT	5 cut(s)	61, 197, 815, 1232, 1757
HpyCH4IV	ACGT	2 cut(s)	91, 1303
HpyF10VI	GCNNNNNNNGC	4 cut(s)	320, 325, 1199, 1595
HpyF3I	CTNAG	5 cut(s)	108, 300, 429, 1085, 1358
HpySE526I	ACGT	2 cut(s)	91, 1303
Hsp92I	GRCGYC	3 cut(s)	101, 314, 654
Hsp92II	CATG	5 cut(s)	648, 679, 1218, 1289, 1375
HspAI	GCGC	3 cut(s)	284, 953, 1710
Kpn2I	TCCGGA	2 cut(s)	358, 1767
KroI	GCCGGC	1 cut(s)	148
KroNI	GCCGGC	1 cut(s)	150
Kzo9I	GATC	4 cut(s)	971, 1093, 1701, 1764
LguI	GCTCTTC	1 cut(s)	1338
LmnI	GCTCC	7 cut(s)	376, 382, 425, 999, 1579, 1770, 1778
Lsp1109I	GCAGC	2 cut(s)	1319, 1547
LweI	GCATC	4 cut(s)	631, 631, 736, 850
MaeI	CTAG	7 cut(s)	168, 320, 720, 849, 1098, 1133, 1247
MaeII	ACGT	2 cut(s)	91, 1303
MaeIII	GTNAC	4 cut(s)	61, 815, 1622, 1759
MalI	GATC	4 cut(s)	973, 1095, 1703, 1766
MboI	GATC	4 cut(s)	971, 1093, 1701, 1764
MfeI	CAATTG	3 cut(s)	567, 1556, 1638
MflI	RGATCY	1 cut(s)	971
MhlI	GDGCHC	7 cut(s)	185, 441, 507, 813, 1054, 1366, 1775
MlyI	GAGTC	4 cut(s)	247, 358, 1469, 1638
MmeI	TCCRAC	1 cut(s)	913
Mph1103I	ATGCAT	1 cut(s)	646
MroI	TCCGGA	2 cut(s)	358, 1767
MroNI	GCCGGC	1 cut(s)	148
MroXI	GAANNNNTTC	2 cut(s)	419, 576
MseI	TTAA	6 cut(s)	528, 773, 821, 921, 1314, 1661
MslI	CAYNNNNRTG	2 cut(s)	674, 761
MspCI	CTTAAG	2 cut(s)	772, 1313
MspI	CCGG	8 cut(s)	114, 149, 239, 257, 296, 359, 380, 1768
MspR9I	CCNGG	5 cut(s)	115, 154, 258, 515, 1117
MunI	CAATTG	3 cut(s)	567, 1556, 1638
MvaI	CCWGG	3 cut(s)	154, 515, 1117
MvnI	CGCG	1 cut(s)	284
MwoI	GCNNNNNNNGC	4 cut(s)	320, 325, 1199, 1595
NaeI	GCCGGC	1 cut(s)	150
NciI	CCSGG	2 cut(s)	115, 258
NcoI	CCATGG	1 cut(s)	1214
NdeII	GATC	4 cut(s)	971, 1093, 1701, 1764
NgoMIV	GCCGGC	1 cut(s)	148
NheI	GCTAGC	1 cut(s)	319
NlaIII	CATG	5 cut(s)	648, 679, 1218, 1289, 1375
NlaIV	GGNNCC	4 cut(s)	127, 378, 1383, 1494
NmeAIII	GCCGAG	2 cut(s)	204, 1647
NmuCI	GTSAC	2 cut(s)	815, 1759
NsiI	ATGCAT	1 cut(s)	646
NspI	RCATGY	1 cut(s)	648
NspV	TTCGAA	2 cut(s)	539, 1521
PciSI	GCTCTTC	1 cut(s)	1338
PcsI	WCGNNNNNNNCGW	1 cut(s)	209
PdiI	GCCGGC	1 cut(s)	150
PdmI	GAANNNNTTC	2 cut(s)	419, 576
PfeI	GAWTC	2 cut(s)	355, 1061
PflFI	GACNNNGTC	1 cut(s)	103
PflMI	CCANNNNNTGG	2 cut(s)	520, 1558
PkrI	GCNGC	3 cut(s)	1309, 1537, 1540
PleI	GAGTC	4 cut(s)	247, 357, 1468, 1637
PpsI	GAGTC	4 cut(s)	247, 357, 1468, 1637
PshAI	GACNNNNGTC	2 cut(s)	1193, 1233
Psp124BI	GAGCTC	2 cut(s)	441, 1775
Psp6I	CCWGG	3 cut(s)	152, 513, 1115
PspEI	GGTNACC	1 cut(s)	1622
PspGI	CCWGG	3 cut(s)	152, 513, 1115
PspN4I	GGNNCC	4 cut(s)	127, 378, 1383, 1494
PspPI	GGNCC	5 cut(s)	4, 126, 511, 1564, 1670
PstNI	CAGNNNCTG	1 cut(s)	391
PsuI	RGATCY	1 cut(s)	971
PsyI	GACNNNGTC	1 cut(s)	103
RsaI	GTAC	4 cut(s)	410, 434, 1549, 1594
RsaNI	GTAC	4 cut(s)	409, 433, 1548, 1593
RseI	CAYNNNNRTG	2 cut(s)	674, 761
SacI	GAGCTC	2 cut(s)	441, 1775
SalI	GTCGAC	1 cut(s)	250
SapI	GCTCTTC	1 cut(s)	1338
SaqAI	TTAA	6 cut(s)	528, 773, 821, 921, 1314, 1661
SatI	GCNGC	3 cut(s)	1308, 1536, 1539
Sau3AI	GATC	4 cut(s)	971, 1093, 1701, 1764
Sau96I	GGNCC	5 cut(s)	4, 126, 511, 1564, 1670
SchI	GAGTC	4 cut(s)	247, 358, 1469, 1638
ScrFI	CCNGG	5 cut(s)	115, 154, 258, 515, 1117
SduI	GDGCHC	7 cut(s)	185, 441, 507, 813, 1054, 1366, 1775
SfaNI	GCATC	4 cut(s)	631, 631, 736, 850
SfcI	CTRYAG	2 cut(s)	1174, 1430
SfuI	TTCGAA	2 cut(s)	539, 1521
SinI	GGWCC	1 cut(s)	511
SmiMI	CAYNNNNRTG	2 cut(s)	674, 761
SmlI	CTYRAG	3 cut(s)	772, 983, 1313
SmoI	CTYRAG	3 cut(s)	772, 983, 1313
SsiI	CCGC	6 cut(s)	274, 374, 726, 1496, 1539, 1596
SspMI	CTAG	7 cut(s)	168, 320, 720, 849, 1098, 1133, 1247
SstI	GAGCTC	2 cut(s)	441, 1775
StyD4I	CCNGG	5 cut(s)	113, 152, 256, 513, 1115
StyI	CCWWGG	3 cut(s)	1140, 1214, 1732
TaaI	ACNGT	5 cut(s)	61, 197, 815, 1232, 1757
TaiI	ACGT	2 cut(s)	94, 1306
TaqI	TCGA	5 cut(s)	161, 251, 539, 1104, 1521
TauI	GCSGC	1 cut(s)	1541
TfiI	GAWTC	2 cut(s)	355, 1061
Tru1I	TTAA	6 cut(s)	528, 773, 821, 921, 1314, 1661
Tru9I	TTAA	6 cut(s)	528, 773, 821, 921, 1314, 1661
TscAI	CASTG	2 cut(s)	14, 1086
TseFI	GTSAC	2 cut(s)	815, 1759
TseI	GCWGC	2 cut(s)	1307, 1535
Tsp45I	GTSAC	2 cut(s)	815, 1759
TspDTI	ATGAA	6 cut(s)	774, 884, 927, 1360, 1469, 1529
TspGWI	ACGGA	5 cut(s)	70, 279, 1064, 1352, 1731
TspRI	CASTG	2 cut(s)	14, 1086
Tth111I	GACNNNGTC	1 cut(s)	103
Van91I	CCANNNNNTGG	2 cut(s)	520, 1558
Vha464I	CTTAAG	2 cut(s)	772, 1313
VneI	GTGCAC	1 cut(s)	809
VpaK11BI	GGWCC	1 cut(s)	511
XapI	RAATTY	8 cut(s)	418, 632, 711, 832, 1068, 1438, 1526, 1745
XbaI	TCTAGA	4 cut(s)	719, 848, 1132, 1246
XceI	RCATGY	1 cut(s)	648
XmiI	GTMKAC	1 cut(s)	251
XmnI	GAANNNNTTC	2 cut(s)	419, 576
XspI	CTAG	7 cut(s)	168, 320, 720, 849, 1098, 1133, 1247
Zsp2I	ATGCAT	1 cut(s)	646

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.