Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0012710

Physical Location & Seq

Genomic Coordinates Reverse (-)

22753927 .. 22760346

Gene Structure 6420 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1156500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1803 bp

ATGGGCCAGTGCTACGGCAAAACCATTCCCACCACCGAAAATGACCCCAACGGCATCAACGTAACTACAATCACTACCTCCGTCCACCAACCTCAAACGACGCCGTCGCAGCAGGCCGGGCAGAACGGGGCCCTCAGCGTAAAGAACACGCCGGCCCGGTCGTCGAACCCTAGCCCATGGCCGAGCCCGTACCCGCACGGCGTTTCTTCCAGCCCTTTACCTGCAGGAGTGTCGCCGTCTCCGGCGAGGTCGTCGACTCCGGGGAGGTTTTTCCGGCGGAGGTTTGCGCCTCCGTCTCCGGCGAAGCACATAAAGGCGTCGCTAGCGAAGCGGTTCGGGAAGCCGAAGGCGAGTCCGATTCCGGAGGAGGGCGGGGCGGAGCCGGAGCCGGAGCAGTCGCTGGACAAGAGTTTCGGGTATGGGAAGAATTTTGGAGCTAAGTATGAGCTCGGGAAGGAGGTGGGGCGAGGGCATTTTGGTCATACTTGCTCTGGTAGGGGAAAGAAGGGTGAGCTCAAGGACCAGCCTGTGGCTGTTAAGATCATTTCGAAAGCCAAGATGACGACAGCAATATCGATTGAAGATGTTCGTAGAGAGGTGAAGATATTGAAAGCTCTATCTGGGCATAAGCATCTTGTAAAATTTTATGATGCATGTGAAGACGCCAATAATGTTTACATTGTCATGGAATTGTGTGAAGGGGGGGAACTTCTCGACAGAATTTTATCTAGAGGTGGAAGGTATGCAGAGGAAGATGCAAAACACATAGTTGTGCAAATTTTAAGTGTTGTTGCATTTTGTCATCTTCAAGGCGTTGTGCACCGTGACTTAAAGCCAGAGAATTTCCTTTTCGCTACTAGAAGTGAAGATGCTGATATGAAGCTTATTGACTTTGGTCTTTCTGACTTTATCAGACCAGATGAAAGACTTAATGATATTGTTGGAAGTGCATACTATGTTGCGCCTGAAGTCCTCCATAGATCTTATAGTCTTGAGGGAGATATATGGAGCATTGGTGTTATCACCTATATCCTGCTATGCGGAAGCCGACCTTTCTGGGCACGAACTGAATCTGGAATTTTCCGTGCAGTGCTACGAGCTGATCCTAGTTTTGATGATTTACCCTGGCCTACTATGTCTCTAGAAGCCAAGGACTTCGTAAGAAGACTTTTGAACAAGGACTACAGAAAAAGAATGACTGCTGTCCAAGCCTTATGTCACCCATGGTTGCGAGATGACAGTTGTCCTCTTCCTTTGGATATATTGATACATAAATTAGTCAAATCATATCTCCAAGCTACGCCATTTAAACGTGCAGCACTTAAGTCTCTTTCCAAAGCTTTGACGGAGGATGAGCTGATATATCTAAGAGCACAATTCATGCTTTTGGAACCAGATAATGATGAGCGTGTCTCTCTTCATAATTTCAAAATGGCTCTACAAAGAAATTCAACTGATGCGATGAAGGAGTCAAGGGTTGTTGATATCCTAAGTGCGATGGAACCGCTTGCTTATAGAACAATGGACTTTGAAGAATTTTGCGCAGCCGCAATCAGTACCCATCAATTGGAGGCCCTGGAAGGGTGGGAGCAGATAGCCTGTACTGCTTTTGAGCATTTTGAACGGGAGGGTAACCGAGTCATATCAATTGAAGAACTGGCAAGAGAATTGAATCTCGGCCCTTCTGCCTACTCTATCCTCCGAGATTGGATCAGAAGCGCAGACGGAAAGCTCAATTTCCTTGGATATACAAAATTTTTGCATGGTGTGACGATCCGTAGCTCCAACACCAGACACCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010150 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

Pkinase

Protein Analysis

601

Amino Acids

67.04

Weight (kDa)

8.37

Isoelectric Point (pI)

52.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	148 - 410	8.3e-70	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	149 - 366	4.9e-34	Protein tyrosine and serine/threonine kinase
Kdo	PF06293	221 - 301	1e-06	Lipopolysaccharide kinase (Kdo/WaaP) family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0012710)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46700 AT2G46700 AT2G46700
fragaria_vesca	FvH4_7g17350 FvH4_7g17350
malus_domestica	MD01G1086500.v1.1 MD07G1156500.v1.1
prunus_persica	Prupe.2G194900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0360791
rosa_laevigata	RLG00000027786
rosa_multiflora	Rmu_sc0000692.1_g000010
rosa_roxburghii	Rroxscaffold_4G00295810
rosa_rugosa	Rorug01G0281500
rosa_samantha	Rh1AG293500 Rh1BG258100 Rh1CG276000 Rh1DG288400
rosa_wichuraiana	Rw1G025970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1543
Acc36I	ACCTGC	1 cut(s)	229
AccB7I	CCANNNNNTGG	2 cut(s)	529, 1567
AccI	GTMKAC	1 cut(s)	254
AccIII	TCCGGA	1 cut(s)	361
AciI	CCGC	8 cut(s)	194, 277, 331, 372, 377, 1041, 1505, 1548
AclWI	GGATC	3 cut(s)	1097, 1718, 1768
AcoI	YGGCCR	1 cut(s)	179
AcsI	RAATTY	9 cut(s)	427, 641, 720, 777, 841, 1077, 1447, 1535, 1754
AcuI	CTGAAG	1 cut(s)	987
AcyI	GRCGYC	3 cut(s)	101, 317, 663
AfaI	GTAC	3 cut(s)	191, 1558, 1603
AfiI	CCNNNNNNNGG	4 cut(s)	368, 529, 1567, 1581
AflII	CTTAAG	1 cut(s)	1322
AjnI	CCWGG	2 cut(s)	1124, 1575
Alw21I	GWGCWC	4 cut(s)	450, 516, 822, 1375
Alw26I	GTCTC	5 cut(s)	243, 300, 1143, 1332, 1417
Alw44I	GTGCAC	1 cut(s)	818
AlwI	GGATC	3 cut(s)	1097, 1718, 1768
AlwNI	CAGNNNCTG	1 cut(s)	400
Ama87I	CYCGRG	1 cut(s)	449
Aor13HI	TCCGGA	1 cut(s)	361
AoxI	GGCC	8 cut(s)	4, 114, 129, 153, 179, 1127, 1572, 1678
ApaI	GGGCCC	1 cut(s)	133
ApaLI	GTGCAC	1 cut(s)	818
ApeKI	GCWGC	3 cut(s)	109, 1316, 1544
ApoI	RAATTY	9 cut(s)	427, 641, 720, 777, 841, 1077, 1447, 1535, 1754
ArsI	GACNNNNNNTTYG	2 cut(s)	395, 427
Asp700I	GAANNNNTTC	2 cut(s)	332, 585
AspLEI	GCGC	4 cut(s)	289, 964, 1544, 1721
AspS9I	GGNCC	7 cut(s)	4, 129, 130, 154, 520, 1573, 1679
AsuC2I	CCSGG	3 cut(s)	118, 157, 261
AsuHPI	GGTGA	4 cut(s)	521, 610, 1015, 1211
AsuII	TTCGAA	1 cut(s)	548
AsuNHI	GCTAGC	1 cut(s)	322
AvaI	CYCGRG	1 cut(s)	449
AvaII	GGWCC	1 cut(s)	520
BaeGI	GKGCMC	3 cut(s)	133, 822, 1063
BanII	GRGCYC	4 cut(s)	133, 188, 450, 516
BbsI	GAAGAC	2 cut(s)	666, 1171
Bbv12I	GWGCWC	4 cut(s)	450, 516, 822, 1375
BbvCI	CCTCAGC	1 cut(s)	134
BbvI	GCAGC	3 cut(s)	121, 1328, 1556
BccI	CCATC	2 cut(s)	1492, 1569
BceAI	ACGGC	5 cut(s)	31, 67, 88, 214, 220
BcgI	CGANNNNNNTGC	2 cut(s)	213, 247
BciT130I	CCWGG	2 cut(s)	1126, 1577
BcnI	CCSGG	3 cut(s)	118, 157, 261
BcoDI	GTCTC	5 cut(s)	243, 300, 1143, 1332, 1417
BfaI	CTAG	6 cut(s)	171, 323, 729, 858, 1107, 1142
BfmI	CTRYAG	2 cut(s)	222, 1183
BfrI	CTTAAG	1 cut(s)	1322
BfuAI	ACCTGC	1 cut(s)	229
BglII	AGATCT	1 cut(s)	980
BisI	GCNGC	4 cut(s)	110, 1317, 1545, 1548
BlsI	GCNGC	4 cut(s)	111, 1318, 1546, 1549
Bme1390I	CCNGG	5 cut(s)	118, 157, 261, 1126, 1577
Bme18I	GGWCC	1 cut(s)	520
BmeT110I	CYCGRG	1 cut(s)	449
BmgT120I	GGNCC	7 cut(s)	4, 129, 130, 154, 520, 1573, 1679
BmiI	GGNNCC	6 cut(s)	130, 131, 381, 387, 1392, 1503
BmrFI	CCNGG	5 cut(s)	118, 157, 261, 1126, 1577
BmsI	GCATC	6 cut(s)	63, 640, 640, 745, 859, 1447
BmtI	GCTAGC	1 cut(s)	326
BoxI	GACNNNNGTC	3 cut(s)	894, 1202, 1242
BpiI	GAAGAC	2 cut(s)	666, 1171
BplI	GAGNNNNNCTC	2 cut(s)	1397, 1429
Bpu10I	CCTNAGC	1 cut(s)	134
Bpu14I	TTCGAA	1 cut(s)	548
BpuEI	CTTGAG	2 cut(s)	500, 1013
BpuMI	CCSGG	3 cut(s)	118, 157, 261
Bsa29I	ATCGAT	1 cut(s)	575
BsaHI	GRCGYC	3 cut(s)	101, 317, 663
BsaJI	CCNNGG	7 cut(s)	176, 260, 1124, 1149, 1223, 1575, 1741
BsaWI	WCCGGW	1 cut(s)	361
BsaXI	ACNNNNNCTCC	2 cut(s)	1000, 1030
Bsc4I	CCNNNNNNNGG	4 cut(s)	368, 529, 1567, 1581
Bse118I	RCCGGY	1 cut(s)	151
Bse1I	ACTGG	2 cut(s)	7, 1662
BseAI	TCCGGA	1 cut(s)	361
BseBI	CCWGG	2 cut(s)	1126, 1577
BseCI	ATCGAT	1 cut(s)	575
BseDI	CCNNGG	7 cut(s)	176, 260, 1124, 1149, 1223, 1575, 1741
BseGI	GGATG	1 cut(s)	1357
BseLI	CCNNNNNNNGG	4 cut(s)	368, 529, 1567, 1581
BseMII	CTCAG	1 cut(s)	148
BseNI	ACTGG	2 cut(s)	7, 1662
BseRI	GAGGAG	1 cut(s)	380
BseSI	GKGCMC	3 cut(s)	133, 822, 1063
BseXI	GCAGC	3 cut(s)	121, 1328, 1556
BsgI	GTGCAG	2 cut(s)	1107, 1335
Bsh1285I	CGRYCG	1 cut(s)	161
BshFI	GGCC	8 cut(s)	6, 116, 131, 155, 181, 1129, 1574, 1680
BshVI	ATCGAT	1 cut(s)	575
BsiEI	CGRYCG	1 cut(s)	161
BsiHKAI	GWGCWC	4 cut(s)	450, 516, 822, 1375
BsiHKCI	CYCGRG	1 cut(s)	449
BslI	CCNNNNNNNGG	4 cut(s)	368, 529, 1567, 1581
BsmAI	GTCTC	5 cut(s)	243, 300, 1143, 1332, 1417
BsmBI	CGTCTC	2 cut(s)	243, 300
BsnI	GGCC	8 cut(s)	6, 116, 131, 155, 181, 1129, 1574, 1680
BsoBI	CYCGRG	1 cut(s)	449
Bsp119I	TTCGAA	1 cut(s)	548
Bsp120I	GGGCCC	1 cut(s)	129
Bsp1286I	GDGCHC	7 cut(s)	133, 188, 450, 516, 822, 1063, 1375
Bsp13I	TCCGGA	1 cut(s)	361
Bsp143I	GATC	5 cut(s)	540, 980, 1102, 1710, 1773
Bsp19I	CCATGG	2 cut(s)	176, 1223
BspACI	CCGC	8 cut(s)	194, 277, 331, 372, 377, 1041, 1505, 1548
BspANI	GGCC	8 cut(s)	6, 116, 131, 155, 181, 1129, 1574, 1680
BspCNI	CTCAG	1 cut(s)	147
BspDI	ATCGAT	1 cut(s)	575
BspEI	TCCGGA	1 cut(s)	361
BspLI	GGNNCC	6 cut(s)	130, 131, 381, 387, 1392, 1503
BspMAI	CTGCAG	1 cut(s)	226
BspMI	ACCTGC	1 cut(s)	229
BspOI	GCTAGC	1 cut(s)	326
BspPI	GGATC	3 cut(s)	1097, 1718, 1768
BspT104I	TTCGAA	1 cut(s)	548
BspTI	CTTAAG	1 cut(s)	1322
BsrFI	RCCGGY	1 cut(s)	151
BsrI	ACTGG	2 cut(s)	7, 1662
BssAI	RCCGGY	1 cut(s)	151
BssECI	CCNNGG	7 cut(s)	176, 260, 1124, 1149, 1223, 1575, 1741
BssMI	GATC	5 cut(s)	540, 980, 1102, 1710, 1773
BssNI	GRCGYC	3 cut(s)	101, 317, 663
BssT1I	CCWWGG	4 cut(s)	176, 1149, 1223, 1741
Bst2UI	CCWGG	2 cut(s)	1126, 1577
Bst4CI	ACNGT	2 cut(s)	824, 1241
Bst6I	CTCTTC	2 cut(s)	1254, 1422
BstACI	GRCGYC	3 cut(s)	101, 317, 663
BstAFI	CTTAAG	1 cut(s)	1322
BstBI	TTCGAA	1 cut(s)	548
BstC8I	GCNNGC	4 cut(s)	114, 153, 324, 1509
BstDEI	CTNAG	4 cut(s)	134, 438, 1367, 1490
BstDSI	CCRYGG	2 cut(s)	176, 1223
BstEII	GGTNACC	1 cut(s)	1631
BstF5I	GGATG	1 cut(s)	1357
BstHHI	GCGC	4 cut(s)	289, 964, 1544, 1721
BstKTI	GATC	5 cut(s)	543, 983, 1105, 1713, 1776
BstMAI	GTCTC	5 cut(s)	243, 300, 1143, 1332, 1417
BstMBI	GATC	5 cut(s)	540, 980, 1102, 1710, 1773
BstMCI	CGRYCG	1 cut(s)	161
BstMWI	GCNNNNNNNGC	6 cut(s)	109, 118, 323, 328, 1208, 1604
BstNI	CCWGG	2 cut(s)	1126, 1577
BstNSI	RCATGY	1 cut(s)	657
BstPAI	GACNNNNGTC	3 cut(s)	894, 1202, 1242
BstPI	GGTNACC	1 cut(s)	1631
BstSCI	CCNGG	5 cut(s)	116, 155, 259, 1124, 1575
BstSFI	CTRYAG	2 cut(s)	222, 1183
BstSLI	GKGCMC	3 cut(s)	133, 822, 1063
BstV1I	GCAGC	3 cut(s)	121, 1328, 1556
BstV2I	GAAGAC	2 cut(s)	666, 1171
BstX2I	RGATCY	1 cut(s)	980
BstYI	RGATCY	1 cut(s)	980
Bsu15I	ATCGAT	1 cut(s)	575
BsuRI	GGCC	8 cut(s)	6, 116, 131, 155, 181, 1129, 1574, 1680
BsuTUI	ATCGAT	1 cut(s)	575
BtgI	CCRYGG	2 cut(s)	176, 1223
BtgZI	GCGATG	2 cut(s)	1475, 1511
BtsCI	GGATG	1 cut(s)	1357
BtsI	GCAGTG	1 cut(s)	1095
BtsIMutI	CAGTG	2 cut(s)	14, 1095
BveI	ACCTGC	1 cut(s)	229
Cac8I	GCNNGC	4 cut(s)	114, 153, 324, 1509
CaiI	CAGNNNCTG	1 cut(s)	400
CfoI	GCGC	4 cut(s)	289, 964, 1544, 1721
Cfr10I	RCCGGY	1 cut(s)	151
Cfr13I	GGNCC	7 cut(s)	4, 129, 130, 154, 520, 1573, 1679
ClaI	ATCGAT	1 cut(s)	575
CseI	GACGC	3 cut(s)	109, 306, 671
Csp6I	GTAC	3 cut(s)	190, 1557, 1602
CviAII	CATG	6 cut(s)	177, 654, 685, 1224, 1381, 1763
CviQI	GTAC	3 cut(s)	190, 1557, 1602
DdeI	CTNAG	4 cut(s)	134, 438, 1367, 1490
DpnI	GATC	5 cut(s)	542, 982, 1104, 1712, 1775
DpnII	GATC	5 cut(s)	540, 980, 1102, 1710, 1773
DraI	TTTAAA	1 cut(s)	1309
EaeI	YGGCCR	1 cut(s)	179
Eam1104I	CTCTTC	2 cut(s)	1254, 1422
EarI	CTCTTC	2 cut(s)	1254, 1422
EciI	GGCGGA	2 cut(s)	292, 392
Ecl136II	GAGCTC	2 cut(s)	448, 514
Eco130I	CCWWGG	4 cut(s)	176, 1149, 1223, 1741
Eco24I	GRGCYC	4 cut(s)	133, 188, 450, 516
Eco32I	GATATC	1 cut(s)	1486
Eco47I	GGWCC	1 cut(s)	520
Eco53kI	GAGCTC	2 cut(s)	448, 514
Eco57I	CTGAAG	1 cut(s)	987
Eco88I	CYCGRG	1 cut(s)	449
Eco91I	GGTNACC	1 cut(s)	1631
EcoICRI	GAGCTC	2 cut(s)	448, 514
EcoO109I	RGGNCCY	3 cut(s)	129, 130, 1573
EcoO65I	GGTNACC	1 cut(s)	1631
EcoRII	CCWGG	2 cut(s)	1124, 1575
EcoRV	GATATC	1 cut(s)	1486
EcoT14I	CCWWGG	4 cut(s)	176, 1149, 1223, 1741
EcoT22I	ATGCAT	1 cut(s)	655
EcoT38I	GRGCYC	4 cut(s)	133, 188, 450, 516
ErhI	CCWWGG	4 cut(s)	176, 1149, 1223, 1741
Esp3I	CGTCTC	2 cut(s)	243, 300
FaeI	CATG	6 cut(s)	180, 657, 688, 1227, 1384, 1766
FalI	AAGNNNNNCTT	2 cut(s)	1036, 1068
FatI	CATG	6 cut(s)	176, 653, 684, 1223, 1380, 1762
FauI	CCCGC	2 cut(s)	201, 365
FblI	GTMKAC	1 cut(s)	254
Fnu4HI	GCNGC	4 cut(s)	110, 1317, 1545, 1548
FokI	GGATG	1 cut(s)	1364
FriOI	GRGCYC	4 cut(s)	133, 188, 450, 516
Fsp4HI	GCNGC	4 cut(s)	110, 1317, 1545, 1548
FspBI	CTAG	6 cut(s)	171, 323, 729, 858, 1107, 1142
FspI	TGCGCA	1 cut(s)	1543
GlaI	GCGC	4 cut(s)	288, 963, 1543, 1720
GluI	GCNGC	4 cut(s)	110, 1317, 1545, 1548
HaeIII	GGCC	8 cut(s)	6, 116, 131, 155, 181, 1129, 1574, 1680
HgaI	GACGC	3 cut(s)	109, 306, 671
HhaI	GCGC	4 cut(s)	289, 964, 1544, 1721
Hin1I	GRCGYC	3 cut(s)	101, 317, 663
Hin1II	CATG	6 cut(s)	180, 657, 688, 1227, 1384, 1766
Hin6I	GCGC	4 cut(s)	287, 962, 1542, 1719
HinP1I	GCGC	4 cut(s)	287, 962, 1542, 1719
HincII	GTYRAC	1 cut(s)	255
HindII	GTYRAC	1 cut(s)	255
HindIII	AAGCTT	2 cut(s)	881, 1338
HinfI	GANTC	7 cut(s)	256, 352, 358, 1070, 1469, 1638, 1672
HphI	GGTGA	4 cut(s)	521, 610, 1015, 1211
Hpy166II	GTNNAC	4 cut(s)	85, 255, 676, 820
Hpy188I	TCNGA	5 cut(s)	357, 904, 914, 1703, 1715
Hpy188III	TCNNGA	8 cut(s)	337, 362, 451, 713, 729, 992, 1074, 1142
Hpy8I	GTNNAC	4 cut(s)	85, 255, 676, 820
Hpy99I	CGWCG	5 cut(s)	103, 109, 166, 256, 322
HpyAV	CCTTC	8 cut(s)	340, 448, 499, 692, 732, 1459, 1574, 1692
HpyCH4III	ACNGT	2 cut(s)	824, 1241
HpyCH4IV	ACGT	2 cut(s)	60, 1312
HpyF10VI	GCNNNNNNNGC	6 cut(s)	109, 118, 323, 328, 1208, 1604
HpyF3I	CTNAG	4 cut(s)	134, 438, 1367, 1490
HpySE526I	ACGT	2 cut(s)	60, 1312
Hsp92I	GRCGYC	3 cut(s)	101, 317, 663
Hsp92II	CATG	6 cut(s)	180, 657, 688, 1227, 1384, 1766
HspAI	GCGC	4 cut(s)	287, 962, 1542, 1719
Kpn2I	TCCGGA	1 cut(s)	361
KroI	GCCGGC	1 cut(s)	151
KroNI	GCCGGC	1 cut(s)	153
Kzo9I	GATC	5 cut(s)	540, 980, 1102, 1710, 1773
LmnI	GCTCC	7 cut(s)	379, 385, 391, 434, 1008, 1588, 1787
Lsp1109I	GCAGC	3 cut(s)	121, 1328, 1556
LweI	GCATC	6 cut(s)	63, 640, 640, 745, 859, 1447
MaeI	CTAG	6 cut(s)	171, 323, 729, 858, 1107, 1142
MaeII	ACGT	2 cut(s)	60, 1312
MaeIII	GTNAC	5 cut(s)	61, 824, 1217, 1631, 1768
MalI	GATC	5 cut(s)	542, 982, 1104, 1712, 1775
MboI	GATC	5 cut(s)	540, 980, 1102, 1710, 1773
MfeI	CAATTG	2 cut(s)	1565, 1647
MflI	RGATCY	1 cut(s)	980
MhlI	GDGCHC	7 cut(s)	133, 188, 450, 516, 822, 1063, 1375
MlyI	GAGTC	4 cut(s)	250, 361, 1478, 1647
MmeI	TCCRAC	1 cut(s)	922
Mph1103I	ATGCAT	1 cut(s)	655
MroI	TCCGGA	1 cut(s)	361
MroNI	GCCGGC	1 cut(s)	151
MroXI	GAANNNNTTC	2 cut(s)	332, 585
MseI	TTAA	6 cut(s)	537, 782, 830, 930, 1308, 1323
MslI	CAYNNNNRTG	2 cut(s)	683, 770
MspCI	CTTAAG	1 cut(s)	1322
MspR9I	CCNGG	5 cut(s)	118, 157, 261, 1126, 1577
MunI	CAATTG	2 cut(s)	1565, 1647
MvaI	CCWGG	2 cut(s)	1126, 1577
MwoI	GCNNNNNNNGC	6 cut(s)	109, 118, 323, 328, 1208, 1604
NaeI	GCCGGC	1 cut(s)	153
NciI	CCSGG	3 cut(s)	118, 157, 261
NcoI	CCATGG	2 cut(s)	176, 1223
NdeII	GATC	5 cut(s)	540, 980, 1102, 1710, 1773
NgoMIV	GCCGGC	1 cut(s)	151
NheI	GCTAGC	1 cut(s)	322
NlaIII	CATG	6 cut(s)	180, 657, 688, 1227, 1384, 1766
NlaIV	GGNNCC	6 cut(s)	130, 131, 381, 387, 1392, 1503
NmeAIII	GCCGAG	2 cut(s)	207, 1656
NmuCI	GTSAC	3 cut(s)	824, 1217, 1768
NsbI	TGCGCA	1 cut(s)	1543
NsiI	ATGCAT	1 cut(s)	655
NspI	RCATGY	1 cut(s)	657
NspV	TTCGAA	1 cut(s)	548
PcsI	WCGNNNNNNNCGW	1 cut(s)	57
PdiI	GCCGGC	1 cut(s)	153
PdmI	GAANNNNTTC	2 cut(s)	332, 585
PfeI	GAWTC	3 cut(s)	358, 1070, 1672
PflFI	GACNNNGTC	1 cut(s)	103
PflMI	CCANNNNNTGG	2 cut(s)	529, 1567
PkrI	GCNGC	4 cut(s)	111, 1318, 1546, 1549
PleI	GAGTC	4 cut(s)	250, 360, 1477, 1646
PpsI	GAGTC	4 cut(s)	250, 360, 1477, 1646
PshAI	GACNNNNGTC	3 cut(s)	894, 1202, 1242
Psp124BI	GAGCTC	2 cut(s)	450, 516
Psp6I	CCWGG	2 cut(s)	1124, 1575
PspEI	GGTNACC	1 cut(s)	1631
PspGI	CCWGG	2 cut(s)	1124, 1575
PspN4I	GGNNCC	6 cut(s)	130, 131, 381, 387, 1392, 1503
PspOMI	GGGCCC	1 cut(s)	129
PspPI	GGNCC	7 cut(s)	4, 129, 130, 154, 520, 1573, 1679
PsrI	GAACNNNNNNTAC	2 cut(s)	1166, 1198
PstI	CTGCAG	1 cut(s)	226
PstNI	CAGNNNCTG	1 cut(s)	400
PsuI	RGATCY	1 cut(s)	980
PsyI	GACNNNGTC	1 cut(s)	103
RsaI	GTAC	3 cut(s)	191, 1558, 1603
RsaNI	GTAC	3 cut(s)	190, 1557, 1602
RseI	CAYNNNNRTG	2 cut(s)	683, 770
SacI	GAGCTC	2 cut(s)	450, 516
SalI	GTCGAC	1 cut(s)	253
SaqAI	TTAA	6 cut(s)	537, 782, 830, 930, 1308, 1323
SatI	GCNGC	4 cut(s)	110, 1317, 1545, 1548
Sau3AI	GATC	5 cut(s)	540, 980, 1102, 1710, 1773
Sau96I	GGNCC	7 cut(s)	4, 129, 130, 154, 520, 1573, 1679
SbfI	CCTGCAGG	1 cut(s)	226
SchI	GAGTC	4 cut(s)	250, 361, 1478, 1647
ScrFI	CCNGG	5 cut(s)	118, 157, 261, 1126, 1577
SdaI	CCTGCAGG	1 cut(s)	226
SduI	GDGCHC	7 cut(s)	133, 188, 450, 516, 822, 1063, 1375
SfaNI	GCATC	6 cut(s)	63, 640, 640, 745, 859, 1447
SfcI	CTRYAG	2 cut(s)	222, 1183
SfuI	TTCGAA	1 cut(s)	548
SinI	GGWCC	1 cut(s)	520
SmiMI	CAYNNNNRTG	2 cut(s)	683, 770
SmlI	CTYRAG	3 cut(s)	515, 992, 1322
SmoI	CTYRAG	3 cut(s)	515, 992, 1322
Sse8387I	CCTGCAGG	1 cut(s)	226
SsiI	CCGC	8 cut(s)	194, 277, 331, 372, 377, 1041, 1505, 1548
SspMI	CTAG	6 cut(s)	171, 323, 729, 858, 1107, 1142
SstI	GAGCTC	2 cut(s)	450, 516
StyD4I	CCNGG	5 cut(s)	116, 155, 259, 1124, 1575
StyI	CCWWGG	4 cut(s)	176, 1149, 1223, 1741
TaaI	ACNGT	2 cut(s)	824, 1241
TaiI	ACGT	2 cut(s)	63, 1315
TaqI	TCGA	5 cut(s)	164, 254, 548, 575, 714
TatI	WGTACW	1 cut(s)	1601
TauI	GCSGC	1 cut(s)	1550
TfiI	GAWTC	3 cut(s)	358, 1070, 1672
Tru1I	TTAA	6 cut(s)	537, 782, 830, 930, 1308, 1323
Tru9I	TTAA	6 cut(s)	537, 782, 830, 930, 1308, 1323
TscAI	CASTG	2 cut(s)	14, 1095
TseFI	GTSAC	3 cut(s)	824, 1217, 1768
TseI	GCWGC	3 cut(s)	109, 1316, 1544
Tsp45I	GTSAC	3 cut(s)	824, 1217, 1768
TspDTI	ATGAA	5 cut(s)	893, 936, 1369, 1409, 1478
TspGWI	ACGGA	6 cut(s)	70, 282, 1073, 1361, 1740, 1766
TspRI	CASTG	2 cut(s)	14, 1095
Tth111I	GACNNNGTC	1 cut(s)	103
Van91I	CCANNNNNTGG	2 cut(s)	529, 1567
Vha464I	CTTAAG	1 cut(s)	1322
VneI	GTGCAC	1 cut(s)	818
VpaK11BI	GGWCC	1 cut(s)	520
XapI	RAATTY	9 cut(s)	427, 641, 720, 777, 841, 1077, 1447, 1535, 1754
XbaI	TCTAGA	2 cut(s)	728, 1141
XceI	RCATGY	1 cut(s)	657
XmiI	GTMKAC	1 cut(s)	254
XmnI	GAANNNNTTC	2 cut(s)	332, 585
XspI	CTAG	6 cut(s)	171, 323, 729, 858, 1107, 1142
Zsp2I	ATGCAT	1 cut(s)	655

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.