Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0012710

Physical Location & Seq

Genomic Coordinates Reverse (-)

52866957 .. 52873005

Gene Structure 6049 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G025970.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1929 bp

ATGGGGCAGTGCTACGGCAAGACTATTCCGACGACGGCGGATAAGGACCCGACGGAGACCGGGACGACTGTCACCACCGGCGCGACGACCGTCACGACAATCAGGACCTCCGTCCAGCACCCGACGCCGTCGCAGGAGGAGGAGCAGCAGAACGGCGCCGGGAGCGTGAAGAGCACGCCGGCGCGGACGTCGATCCCTCAGCCGAGCCCCTGGCCGAGTCCCTACCCGCACGGCGTGTCTGCCAGCCCGCTGCCGGGGGGAGTGTCGCCGTCGCCGGCGAGGTCGTCGACGCCGGGGAGGTTTTTTCGGAGGAGGTTCGCGCCGCCGTCGCCGGCGAAGCACATAAAGGCGTCGCTGGCGAAGCGGTTCGGGCAGAAGCCGAAGGAGAGCCCGATTCCGGAGGAGCAGGGAGTTGAGCAGGAGCAGTCGCTGGATAAGAGCTTTGGGTATGGTAAGAATTTTGGAGCTAAGTATGAGCTTGGGAAAGAGGTCGGGCGAGGGCATTTTGGTCATACTTGCTCCGGTAGAGGCAAGAAAGGCGAGCTCAAGGACAAGCCTTTGGCCGTTAAGATCATCTCCAAAGCCAAGATGACAACGGCCATATCGATTGAAGATGTTCGCCGAGAGGTGAAGATATTGAAAGCTCTATCTGGGCATAAGCATCTTGTAAAATTTTATGATGCATGTGAAGACGCCAATAATGTTTACATTATCATGGAACTATGTGAAGGTGGGGAACTTCTTGACAGAATTTTATCCAGAGGTGGAAAGTATGCAGAAGAAGATGCAAAATTTATAGTTGTGCAAATTCTAAGTGTTGTTGCATTTTGTCATCTTCAAGGCGTCGTGCACCGTGACTTAAAGCCAGAGGTTTCGATATTCAACCTTAATGTCTTAATTATGACTTGGTATCACTTTTTTATCCCCCAATGCATTGCTAATTATATTCTCTTTCTTGCTCAAAAGAACTTCCTTTTCGCTTCTAGAGATGAAGATGCTGATATGAAGCTTATTGATTTTGGTCTTTCTGACTTTATCAGACCAGATGAAAGACTTAATGATATTGTTGGGAGTGCATATTATGTTGCACCTGAAGTCCTTCATAGATCTTATAGCCTTGAGGGAGATATATGGAGCATTGGTGTTATCACCTATATCCTGCTTTGCGGAAGCCGGCCTTTCTGGGCACGAACAGAATCAGGAATTTTCCGTGCGGTGCTAAGAGCTGATCCTAGTTTTGATGATCCACCCTGGCCTTCTATGTCGCACGAGGCCAAGGACTTTGTAAAACGGCTTTTGAACAAGGACTACAGAAAGAGAATGACTGCTGTCCAGGCGTTATCTCACCCATGGTTGCGAGATGATAGTCGTCCTCTCCCTTTAGATATATTGATCTATAAATTAGTCAAATCATATCTGCATGCTACACCTTTCAAACGTGCAGCACTTAAGTCTCTTTCAAAAGCCTTGACGGAGGATGAGCTGATATATCTTAGAGCCCAATTCATGCTTTTGGGACCAGATAAAGATCGACGTATCTCTCTTCATAATTTCAAAACAGCTCTTCAGAGAAATTCAACTGAAGCGATGATGGAGTCGAGGGTTGTTGATATTCTAAATGCAATGGAACCACTTGCATATAGGAAAATGGACTTTGAAGAATTTTGTGCTGCTGCAATTAGCACGCATCAATTGGAGGCCCTCGAAGGATGGGAGCAGATAGCATCTACTGCTTTTGAGTGCTTCGAACGCGATGGCAACCGAGTCATATCGATTGAAGAACTGGCCAGAGAATTAAATCTGGGCACTTCTGCCTATTCAATCCTCAGAGATTGGATCAGAAACACAGATGGAAAACTTAGTTTCATGGGATATACAAAATTTTTGCATGGTGTCACGATCCGTAGCTCCAACACCAGGCACCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010150 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

Pkinase

Protein Analysis

642

Amino Acids

72.15

Weight (kDa)

8.79

Isoelectric Point (pI)

45.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	158 - 452	2e-64	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	159 - 443	6.4e-28	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012710)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46700 AT2G46700 AT2G46700
fragaria_vesca	FvH4_7g17350 FvH4_7g17350
malus_domestica	MD01G1086500.v1.1 MD07G1156500.v1.1
prunus_persica	Prupe.2G194900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0360791
rosa_laevigata	RLG00000027786
rosa_multiflora	Rmu_sc0000692.1_g000010
rosa_roxburghii	Rroxscaffold_4G00295810
rosa_rugosa	Rorug01G0281500
rosa_samantha	Rh1AG293500 Rh1BG258100 Rh1CG276000 Rh1DG288400
rosa_wichuraiana	Rw1G025970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	191
AccB1I	GGYRCC	2 cut(s)	155, 1920
AccI	GTMKAC	1 cut(s)	287
AccII	CGCG	4 cut(s)	83, 184, 320, 1752
AccIII	TCCGGA	1 cut(s)	397
AciI	CCGC	8 cut(s)	38, 184, 227, 248, 323, 364, 1167, 1214
AclWI	GGATC	5 cut(s)	187, 1223, 1238, 1844, 1894
AcoI	YGGCCR	4 cut(s)	212, 561, 597, 1785
AcsI	RAATTY	9 cut(s)	457, 671, 750, 791, 807, 1203, 1573, 1661, 1880
AcuI	CTGAAG	3 cut(s)	1113, 1550, 1602
AcyI	GRCGYC	7 cut(s)	125, 156, 188, 290, 350, 693, 843
AdeI	CACNNNGTG	1 cut(s)	235
AfiI	CCNNNNNNNGG	4 cut(s)	253, 254, 397, 1917
AflII	CTTAAG	1 cut(s)	1448
AjnI	CCWGG	4 cut(s)	209, 1250, 1332, 1916
Alw21I	GWGCWC	3 cut(s)	176, 546, 852
Alw26I	GTCTC	2 cut(s)	50, 1458
Alw44I	GTGCAC	1 cut(s)	848
AlwI	GGATC	5 cut(s)	187, 1223, 1238, 1844, 1894
AlwNI	CAGNNNCTG	1 cut(s)	430
Aor13HI	TCCGGA	1 cut(s)	397
AoxI	GGCC	8 cut(s)	212, 561, 597, 1175, 1253, 1272, 1698, 1785
ApaLI	GTGCAC	1 cut(s)	848
ApeKI	GCWGC	5 cut(s)	145, 250, 1442, 1670, 1673
ApoI	RAATTY	9 cut(s)	457, 671, 750, 791, 807, 1203, 1573, 1661, 1880
Asp700I	GAANNNNTTC	3 cut(s)	365, 615, 1098
AspLEI	GCGC	4 cut(s)	83, 158, 184, 322
AspS9I	GGNCC	4 cut(s)	46, 105, 1517, 1699
AsuC2I	CCSGG	4 cut(s)	61, 160, 255, 294
AsuHPI	GGTGA	4 cut(s)	64, 640, 1141, 1337
AsuII	TTCGAA	1 cut(s)	1746
AvaII	GGWCC	3 cut(s)	46, 105, 1517
BaeGI	GKGCMC	3 cut(s)	852, 1189, 1808
BalI	TGGCCA	1 cut(s)	1787
BanI	GGYRCC	2 cut(s)	155, 1920
BanII	GRGCYC	4 cut(s)	209, 392, 546, 1501
BauI	CACGAG	1 cut(s)	1268
BbsI	GAAGAC	1 cut(s)	696
Bbv12I	GWGCWC	3 cut(s)	176, 546, 852
BbvCI	CCTCAGC	1 cut(s)	198
BbvI	GCAGC	5 cut(s)	157, 237, 1454, 1657, 1660
BccI	CCATC	4 cut(s)	1585, 1704, 1748, 1844
BciT130I	CCWGG	4 cut(s)	211, 1252, 1334, 1918
BcnI	CCSGG	4 cut(s)	61, 160, 255, 294
BcoDI	GTCTC	2 cut(s)	50, 1458
BfaI	CTAG	2 cut(s)	984, 1233
BfmI	CTRYAG	1 cut(s)	1309
BfoI	RGCGCY	1 cut(s)	159
BfrI	CTTAAG	1 cut(s)	1448
BglII	AGATCT	1 cut(s)	1106
BisI	GCNGC	6 cut(s)	146, 251, 323, 1443, 1671, 1674
BlsI	GCNGC	6 cut(s)	147, 252, 324, 1444, 1672, 1675
Bme1390I	CCNGG	8 cut(s)	61, 160, 211, 255, 294, 1252, 1334, 1918
Bme18I	GGWCC	3 cut(s)	46, 105, 1517
BmgT120I	GGNCC	4 cut(s)	46, 105, 1517, 1699
BmiI	GGNNCC	5 cut(s)	48, 157, 1518, 1629, 1922
BmrFI	CCNGG	8 cut(s)	61, 160, 211, 255, 294, 1252, 1334, 1918
BmsI	GCATC	6 cut(s)	670, 670, 775, 985, 1696, 1733
BoxI	GACNNNNGTC	4 cut(s)	68, 89, 110, 1328
BpiI	GAAGAC	1 cut(s)	696
Bpu10I	CCTNAGC	1 cut(s)	198
Bpu14I	TTCGAA	1 cut(s)	1746
BpuEI	CTTGAG	2 cut(s)	530, 1139
BpuMI	CCSGG	4 cut(s)	61, 160, 255, 294
Bsa29I	ATCGAT	2 cut(s)	605, 1772
BsaBI	GATNNNNATC	1 cut(s)	1527
BsaHI	GRCGYC	7 cut(s)	125, 156, 188, 290, 350, 693, 843
BsaI	GGTCTC	1 cut(s)	50
BsaJI	CCNNGG	6 cut(s)	209, 254, 293, 1250, 1275, 1349
BsaWI	WCCGGW	2 cut(s)	397, 521
BsaXI	ACNNNNNCTCC	2 cut(s)	1126, 1156
Bsc4I	CCNNNNNNNGG	4 cut(s)	253, 254, 397, 1917
Bse118I	RCCGGY	5 cut(s)	77, 178, 274, 331, 1173
Bse1I	ACTGG	1 cut(s)	1788
Bse3DI	GCAATG	2 cut(s)	933, 1629
Bse8I	GATNNNNATC	1 cut(s)	1527
BseAI	TCCGGA	1 cut(s)	397
BseBI	CCWGG	4 cut(s)	211, 1252, 1334, 1918
BseCI	ATCGAT	2 cut(s)	605, 1772
BseDI	CCNNGG	6 cut(s)	209, 254, 293, 1250, 1275, 1349
BseGI	GGATG	2 cut(s)	1483, 1715
BseJI	GATNNNNATC	1 cut(s)	1527
BseLI	CCNNNNNNNGG	4 cut(s)	253, 254, 397, 1917
BseMI	GCAATG	2 cut(s)	933, 1629
BseMII	CTCAG	2 cut(s)	212, 1840
BseNI	ACTGG	1 cut(s)	1788
BseRI	GAGGAG	4 cut(s)	152, 155, 325, 416
BseSI	GKGCMC	3 cut(s)	852, 1189, 1808
BseXI	GCAGC	5 cut(s)	157, 237, 1454, 1657, 1660
BsgI	GTGCAG	1 cut(s)	1461
Bsh1236I	CGCG	4 cut(s)	83, 184, 320, 1752
Bsh1285I	CGRYCG	1 cut(s)	90
BshFI	GGCC	8 cut(s)	214, 563, 599, 1177, 1255, 1274, 1700, 1787
BshNI	GGYRCC	2 cut(s)	155, 1920
BshVI	ATCGAT	2 cut(s)	605, 1772
BsiEI	CGRYCG	1 cut(s)	90
BsiHKAI	GWGCWC	3 cut(s)	176, 546, 852
BslFI	GGGAC	3 cut(s)	76, 204, 1530
BslI	CCNNNNNNNGG	4 cut(s)	253, 254, 397, 1917
BsmAI	GTCTC	2 cut(s)	50, 1458
BsmFI	GGGAC	3 cut(s)	76, 204, 1530
BsnI	GGCC	8 cut(s)	214, 563, 599, 1177, 1255, 1274, 1700, 1787
Bso31I	GGTCTC	1 cut(s)	50
Bsp119I	TTCGAA	1 cut(s)	1746
Bsp1286I	GDGCHC	8 cut(s)	176, 209, 392, 546, 852, 1189, 1501, 1808
Bsp13I	TCCGGA	1 cut(s)	397
Bsp143I	GATC	9 cut(s)	192, 570, 1106, 1228, 1243, 1392, 1528, 1836, 1899
Bsp19I	CCATGG	1 cut(s)	1349
BspACI	CCGC	8 cut(s)	38, 184, 227, 248, 323, 364, 1167, 1214
BspANI	GGCC	8 cut(s)	214, 563, 599, 1177, 1255, 1274, 1700, 1787
BspCNI	CTCAG	2 cut(s)	211, 1839
BspDI	ATCGAT	2 cut(s)	605, 1772
BspEI	TCCGGA	1 cut(s)	397
BspFNI	CGCG	4 cut(s)	83, 184, 320, 1752
BspLI	GGNNCC	5 cut(s)	48, 157, 1518, 1629, 1922
BspPI	GGATC	5 cut(s)	187, 1223, 1238, 1844, 1894
BspQI	GCTCTTC	2 cut(s)	164, 1569
BspT104I	TTCGAA	1 cut(s)	1746
BspT107I	GGYRCC	2 cut(s)	155, 1920
BspTI	CTTAAG	1 cut(s)	1448
BspTNI	GGTCTC	1 cut(s)	50
BsrDI	GCAATG	2 cut(s)	933, 1629
BsrFI	RCCGGY	5 cut(s)	77, 178, 274, 331, 1173
BsrI	ACTGG	1 cut(s)	1788
BssAI	RCCGGY	5 cut(s)	77, 178, 274, 331, 1173
BssECI	CCNNGG	6 cut(s)	209, 254, 293, 1250, 1275, 1349
BssMI	GATC	9 cut(s)	192, 570, 1106, 1228, 1243, 1392, 1528, 1836, 1899
BssNI	GRCGYC	7 cut(s)	125, 156, 188, 290, 350, 693, 843
BssSI	CACGAG	1 cut(s)	1268
BssT1I	CCWWGG	2 cut(s)	1275, 1349
Bst2BI	CACGAG	1 cut(s)	1268
Bst2UI	CCWGG	4 cut(s)	211, 1252, 1334, 1918
Bst4CI	ACNGT	3 cut(s)	70, 91, 854
Bst6I	CTCTTC	3 cut(s)	164, 1548, 1569
BstACI	GRCGYC	7 cut(s)	125, 156, 188, 290, 350, 693, 843
BstAFI	CTTAAG	1 cut(s)	1448
BstAPI	GCANNNNNTGC	1 cut(s)	1730
BstBI	TTCGAA	1 cut(s)	1746
BstDEI	CTNAG	7 cut(s)	198, 468, 812, 1220, 1493, 1826, 1859
BstDSI	CCRYGG	1 cut(s)	1349
BstF5I	GGATG	2 cut(s)	1483, 1715
BstFNI	CGCG	4 cut(s)	83, 184, 320, 1752
BstH2I	RGCGCY	1 cut(s)	159
BstHHI	GCGC	4 cut(s)	83, 158, 184, 322
BstKTI	GATC	9 cut(s)	195, 573, 1109, 1231, 1246, 1395, 1531, 1839, 1902
BstMAI	GTCTC	2 cut(s)	50, 1458
BstMBI	GATC	9 cut(s)	192, 570, 1106, 1228, 1243, 1392, 1528, 1836, 1899
BstMCI	CGRYCG	1 cut(s)	90
BstNI	CCWGG	4 cut(s)	211, 1252, 1334, 1918
BstNSI	RCATGY	2 cut(s)	687, 1424
BstPAI	GACNNNNGTC	4 cut(s)	68, 89, 110, 1328
BstSCI	CCNGG	8 cut(s)	59, 158, 209, 253, 292, 1250, 1332, 1916
BstSFI	CTRYAG	1 cut(s)	1309
BstSLI	GKGCMC	3 cut(s)	852, 1189, 1808
BstUI	CGCG	4 cut(s)	83, 184, 320, 1752
BstV1I	GCAGC	5 cut(s)	157, 237, 1454, 1657, 1660
BstV2I	GAAGAC	1 cut(s)	696
BstX2I	RGATCY	1 cut(s)	1106
BstYI	RGATCY	1 cut(s)	1106
Bsu15I	ATCGAT	2 cut(s)	605, 1772
BsuRI	GGCC	8 cut(s)	214, 563, 599, 1177, 1255, 1274, 1700, 1787
BsuTUI	ATCGAT	2 cut(s)	605, 1772
BtgI	CCRYGG	1 cut(s)	1349
BtgZI	GCGATG	2 cut(s)	1601, 1767
BtsCI	GGATG	2 cut(s)	1483, 1715
BtsI	GCAGTG	1 cut(s)	14
BtsIMutI	CAGTG	1 cut(s)	14
CaiI	CAGNNNCTG	1 cut(s)	430
CfoI	GCGC	4 cut(s)	83, 158, 184, 322
Cfr10I	RCCGGY	5 cut(s)	77, 178, 274, 331, 1173
Cfr13I	GGNCC	4 cut(s)	46, 105, 1517, 1699
ClaI	ATCGAT	2 cut(s)	605, 1772
CseI	GACGC	5 cut(s)	133, 298, 339, 701, 832
CviAII	CATG	7 cut(s)	684, 715, 1350, 1421, 1507, 1867, 1889
DdeI	CTNAG	7 cut(s)	198, 468, 812, 1220, 1493, 1826, 1859
DinI	GGCGCC	1 cut(s)	157
DpnI	GATC	9 cut(s)	194, 572, 1108, 1230, 1245, 1394, 1530, 1838, 1901
DpnII	GATC	9 cut(s)	192, 570, 1106, 1228, 1243, 1392, 1528, 1836, 1899
DraIII	CACNNNGTG	1 cut(s)	235
EaeI	YGGCCR	4 cut(s)	212, 561, 597, 1785
Eam1104I	CTCTTC	3 cut(s)	164, 1548, 1569
EarI	CTCTTC	3 cut(s)	164, 1548, 1569
EciI	GGCGGA	1 cut(s)	53
Ecl136II	GAGCTC	1 cut(s)	544
Eco130I	CCWWGG	2 cut(s)	1275, 1349
Eco24I	GRGCYC	4 cut(s)	209, 392, 546, 1501
Eco31I	GGTCTC	1 cut(s)	50
Eco47I	GGWCC	3 cut(s)	46, 105, 1517
Eco53kI	GAGCTC	1 cut(s)	544
Eco57I	CTGAAG	3 cut(s)	1113, 1550, 1602
EcoICRI	GAGCTC	1 cut(s)	544
EcoO109I	RGGNCCY	3 cut(s)	46, 105, 1699
EcoRII	CCWGG	4 cut(s)	209, 1250, 1332, 1916
EcoT14I	CCWWGG	2 cut(s)	1275, 1349
EcoT22I	ATGCAT	2 cut(s)	685, 935
EcoT38I	GRGCYC	4 cut(s)	209, 392, 546, 1501
EgeI	GGCGCC	1 cut(s)	157
EheI	GGCGCC	1 cut(s)	157
ErhI	CCWWGG	2 cut(s)	1275, 1349
FaeI	CATG	7 cut(s)	687, 718, 1353, 1424, 1510, 1870, 1892
FalI	AAGNNNNNCTT	2 cut(s)	1162, 1194
FaqI	GGGAC	3 cut(s)	76, 204, 1530
FatI	CATG	7 cut(s)	683, 714, 1349, 1420, 1506, 1866, 1888
FauI	CCCGC	2 cut(s)	234, 255
FblI	GTMKAC	1 cut(s)	287
Fnu4HI	GCNGC	6 cut(s)	146, 251, 323, 1443, 1671, 1674
FokI	GGATG	2 cut(s)	1490, 1722
FriOI	GRGCYC	4 cut(s)	209, 392, 546, 1501
Fsp4HI	GCNGC	6 cut(s)	146, 251, 323, 1443, 1671, 1674
FspBI	CTAG	2 cut(s)	984, 1233
GlaI	GCGC	4 cut(s)	82, 157, 183, 321
GluI	GCNGC	6 cut(s)	146, 251, 323, 1443, 1671, 1674
HaeII	RGCGCY	1 cut(s)	159
HaeIII	GGCC	8 cut(s)	214, 563, 599, 1177, 1255, 1274, 1700, 1787
HgaI	GACGC	5 cut(s)	133, 298, 339, 701, 832
HhaI	GCGC	4 cut(s)	83, 158, 184, 322
Hin1I	GRCGYC	7 cut(s)	125, 156, 188, 290, 350, 693, 843
Hin1II	CATG	7 cut(s)	687, 718, 1353, 1424, 1510, 1870, 1892
Hin6I	GCGC	4 cut(s)	81, 156, 182, 320
HinP1I	GCGC	4 cut(s)	81, 156, 182, 320
HincII	GTYRAC	1 cut(s)	288
HindII	GTYRAC	1 cut(s)	288
HindIII	AAGCTT	1 cut(s)	1007
HinfI	GANTC	5 cut(s)	217, 394, 1196, 1595, 1764
HphI	GGTGA	4 cut(s)	64, 640, 1141, 1337
Hpy166II	GTNNAC	3 cut(s)	288, 706, 850
Hpy188I	TCNGA	7 cut(s)	30, 309, 1030, 1040, 1569, 1829, 1841
Hpy188III	TCNNGA	8 cut(s)	94, 103, 398, 743, 759, 984, 1200, 1897
Hpy8I	GTNNAC	3 cut(s)	288, 706, 850
HpyAV	CCTTC	5 cut(s)	376, 722, 1109, 1266, 1700
HpyCH4III	ACNGT	3 cut(s)	70, 91, 854
HpyCH4IV	ACGT	3 cut(s)	188, 1438, 1534
HpyF3I	CTNAG	7 cut(s)	198, 468, 812, 1220, 1493, 1826, 1859
HpySE526I	ACGT	3 cut(s)	188, 1438, 1534
Hsp92I	GRCGYC	7 cut(s)	125, 156, 188, 290, 350, 693, 843
Hsp92II	CATG	7 cut(s)	687, 718, 1353, 1424, 1510, 1870, 1892
HspAI	GCGC	4 cut(s)	81, 156, 182, 320
KasI	GGCGCC	1 cut(s)	155
Kpn2I	TCCGGA	1 cut(s)	397
KroI	GCCGGC	4 cut(s)	178, 274, 331, 1173
KroNI	GCCGGC	4 cut(s)	180, 276, 333, 1175
Kzo9I	GATC	9 cut(s)	192, 570, 1106, 1228, 1243, 1392, 1528, 1836, 1899
LguI	GCTCTTC	2 cut(s)	164, 1569
LmnI	GCTCC	9 cut(s)	142, 162, 403, 421, 464, 524, 1134, 1714, 1913
Lsp1109I	GCAGC	5 cut(s)	157, 237, 1454, 1657, 1660
LweI	GCATC	6 cut(s)	670, 670, 775, 985, 1696, 1733
MaeI	CTAG	2 cut(s)	984, 1233
MaeII	ACGT	3 cut(s)	188, 1438, 1534
MaeIII	GTNAC	4 cut(s)	70, 91, 854, 1894
MalI	GATC	9 cut(s)	194, 572, 1108, 1230, 1245, 1394, 1530, 1838, 1901
MboI	GATC	9 cut(s)	192, 570, 1106, 1228, 1243, 1392, 1528, 1836, 1899
MfeI	CAATTG	1 cut(s)	1691
MflI	RGATCY	1 cut(s)	1106
MhlI	GDGCHC	8 cut(s)	176, 209, 392, 546, 852, 1189, 1501, 1808
MlsI	TGGCCA	1 cut(s)	1787
MluNI	TGGCCA	1 cut(s)	1787
Mly113I	GGCGCC	1 cut(s)	156
MlyI	GAGTC	3 cut(s)	226, 1604, 1773
MmeI	TCCRAC	1 cut(s)	53
Mox20I	TGGCCA	1 cut(s)	1787
Mph1103I	ATGCAT	2 cut(s)	685, 935
MreI	CGCCGGCG	3 cut(s)	178, 274, 331
MroI	TCCGGA	1 cut(s)	397
MroNI	GCCGGC	4 cut(s)	178, 274, 331, 1173
MroXI	GAANNNNTTC	3 cut(s)	365, 615, 1098
MscI	TGGCCA	1 cut(s)	1787
MseI	TTAA	7 cut(s)	567, 860, 888, 896, 1056, 1449, 1796
MslI	CAYNNNNRTG	1 cut(s)	713
Msp20I	TGGCCA	1 cut(s)	1787
MspA1I	CMGCKG	1 cut(s)	250
MspCI	CTTAAG	1 cut(s)	1448
MspR9I	CCNGG	8 cut(s)	61, 160, 211, 255, 294, 1252, 1334, 1918
MunI	CAATTG	1 cut(s)	1691
MvaI	CCWGG	4 cut(s)	211, 1252, 1334, 1918
MvnI	CGCG	4 cut(s)	83, 184, 320, 1752
NaeI	GCCGGC	4 cut(s)	180, 276, 333, 1175
NarI	GGCGCC	1 cut(s)	156
NciI	CCSGG	4 cut(s)	61, 160, 255, 294
NcoI	CCATGG	1 cut(s)	1349
NdeII	GATC	9 cut(s)	192, 570, 1106, 1228, 1243, 1392, 1528, 1836, 1899
NgoMIV	GCCGGC	4 cut(s)	178, 274, 331, 1173
NlaIII	CATG	7 cut(s)	687, 718, 1353, 1424, 1510, 1870, 1892
NlaIV	GGNNCC	5 cut(s)	48, 157, 1518, 1629, 1922
NmeAIII	GCCGAG	3 cut(s)	228, 240, 647
NmuCI	GTSAC	4 cut(s)	70, 91, 854, 1894
NsiI	ATGCAT	2 cut(s)	685, 935
NspI	RCATGY	2 cut(s)	687, 1424
NspV	TTCGAA	1 cut(s)	1746
PaeI	GCATGC	1 cut(s)	1424
PciSI	GCTCTTC	2 cut(s)	164, 1569
PcsI	WCGNNNNNNNCGW	2 cut(s)	92, 602
PdiI	GCCGGC	4 cut(s)	180, 276, 333, 1175
PdmI	GAANNNNTTC	3 cut(s)	365, 615, 1098
PfeI	GAWTC	2 cut(s)	394, 1196
PflFI	GACNNNGTC	1 cut(s)	127
PkrI	GCNGC	6 cut(s)	147, 252, 324, 1444, 1672, 1675
PleI	GAGTC	3 cut(s)	225, 1603, 1772
PluTI	GGCGCC	1 cut(s)	159
PpsI	GAGTC	3 cut(s)	225, 1603, 1772
PpuMI	RGGWCCY	2 cut(s)	46, 105
PshAI	GACNNNNGTC	4 cut(s)	68, 89, 110, 1328
Psp124BI	GAGCTC	1 cut(s)	546
Psp5II	RGGWCCY	2 cut(s)	46, 105
Psp6I	CCWGG	4 cut(s)	209, 1250, 1332, 1916
PspGI	CCWGG	4 cut(s)	209, 1250, 1332, 1916
PspN4I	GGNNCC	5 cut(s)	48, 157, 1518, 1629, 1922
PspPI	GGNCC	4 cut(s)	46, 105, 1517, 1699
PspPPI	RGGWCCY	2 cut(s)	46, 105
PsrI	GAACNNNNNNTAC	2 cut(s)	1292, 1324
PstNI	CAGNNNCTG	1 cut(s)	430
PsuI	RGATCY	1 cut(s)	1106
PsyI	GACNNNGTC	1 cut(s)	127
RseI	CAYNNNNRTG	1 cut(s)	713
SacI	GAGCTC	1 cut(s)	546
SalI	GTCGAC	1 cut(s)	286
SapI	GCTCTTC	2 cut(s)	164, 1569
SaqAI	TTAA	7 cut(s)	567, 860, 888, 896, 1056, 1449, 1796
SatI	GCNGC	6 cut(s)	146, 251, 323, 1443, 1671, 1674
Sau3AI	GATC	9 cut(s)	192, 570, 1106, 1228, 1243, 1392, 1528, 1836, 1899
Sau96I	GGNCC	4 cut(s)	46, 105, 1517, 1699
SchI	GAGTC	3 cut(s)	226, 1604, 1773
ScrFI	CCNGG	8 cut(s)	61, 160, 211, 255, 294, 1252, 1334, 1918
SduI	GDGCHC	8 cut(s)	176, 209, 392, 546, 852, 1189, 1501, 1808
SfaNI	GCATC	6 cut(s)	670, 670, 775, 985, 1696, 1733
SfcI	CTRYAG	1 cut(s)	1309
SfoI	GGCGCC	1 cut(s)	157
SfuI	TTCGAA	1 cut(s)	1746
SgrAI	CRCCGGYG	4 cut(s)	77, 178, 274, 331
SgrDI	CGTCGACG	1 cut(s)	286
SinI	GGWCC	3 cut(s)	46, 105, 1517
SmiMI	CAYNNNNRTG	1 cut(s)	713
SmlI	CTYRAG	3 cut(s)	545, 1118, 1448
SmoI	CTYRAG	3 cut(s)	545, 1118, 1448
SphI	GCATGC	1 cut(s)	1424
SsiI	CCGC	8 cut(s)	38, 184, 227, 248, 323, 364, 1167, 1214
SspDI	GGCGCC	1 cut(s)	155
SspMI	CTAG	2 cut(s)	984, 1233
SstI	GAGCTC	1 cut(s)	546
StyD4I	CCNGG	8 cut(s)	59, 158, 209, 253, 292, 1250, 1332, 1916
StyI	CCWWGG	2 cut(s)	1275, 1349
TaaI	ACNGT	3 cut(s)	70, 91, 854
TaiI	ACGT	3 cut(s)	191, 1441, 1537
TaqI	TCGA	9 cut(s)	191, 287, 605, 875, 1531, 1598, 1704, 1746, 1772
TauI	GCSGC	1 cut(s)	325
TfiI	GAWTC	2 cut(s)	394, 1196
Tru1I	TTAA	7 cut(s)	567, 860, 888, 896, 1056, 1449, 1796
Tru9I	TTAA	7 cut(s)	567, 860, 888, 896, 1056, 1449, 1796
TscAI	CASTG	1 cut(s)	14
TseFI	GTSAC	4 cut(s)	70, 91, 854, 1894
TseI	GCWGC	5 cut(s)	145, 250, 1442, 1670, 1673
Tsp45I	GTSAC	4 cut(s)	70, 91, 854, 1894
TspDTI	ATGAA	7 cut(s)	1005, 1019, 1062, 1091, 1495, 1535, 1855
TspGWI	ACGGA	5 cut(s)	68, 100, 1199, 1487, 1892
TspRI	CASTG	1 cut(s)	14
Tth111I	GACNNNGTC	1 cut(s)	127
Vha464I	CTTAAG	1 cut(s)	1448
VneI	GTGCAC	1 cut(s)	848
VpaK11BI	GGWCC	3 cut(s)	46, 105, 1517
XapI	RAATTY	9 cut(s)	457, 671, 750, 791, 807, 1203, 1573, 1661, 1880
XbaI	TCTAGA	1 cut(s)	983
XceI	RCATGY	2 cut(s)	687, 1424
XmiI	GTMKAC	1 cut(s)	287
XmnI	GAANNNNTTC	3 cut(s)	365, 615, 1098
XspI	CTAG	2 cut(s)	984, 1233
ZraI	GACGTC	1 cut(s)	189
Zsp2I	ATGCAT	2 cut(s)	685, 935

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.