Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0012710

Physical Location & Seq

Genomic Coordinates Forward (+)

15890026 .. 15896238

Gene Structure 6213 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00295810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1725 bp

ATGGGGCAGTGCTACGGCAAGACTATTCCGACGACGGCGGATAAGGACCGGACGGAGACCGGGACGACTGTCACCACCGGCGCGACGACCGTCACGACAATCTCGACCTCCGTCCAGCACCCGACGCCGTCGCAGGAGGAGGAGCAGCAGAACGGCGCCGGGAGCGTGAAGAGCACGCCGGCGCGGACGTCGATCCCTCAGCCGAGCCCCTGGCCGAGTCCCTACCCGCACGGCGTGTCTGCCAGCCCGCTGCCGGGGGGAGTGTCGCCGTCGCCGGCGAGGACGTCGACGCCTGGGAGGTTTTTTCGGAGGAGGTTCGCGCCGCCGTCGCCGGCGAAGCACATAAAGGCGTCGCTGGCGAAGCGGTTCGGGCAGAAGCCGAAGGAGAGCCCGATTCCGGAGGAGCAGGGAGTTGAGCAGGAGCAGTCGCTGGATAAGAGCTTTGGGTATGGTAAGAATTTTGGAGCTAAGTATGAGCTTGGGAAAGAGGTCGGGCGAGGGCATTTTGGTCATACTTGCTCCGGTAGAGGCAAGAAAGGCGAGCTCAAGGACAAGCCTTTGGCCGTTAAGATCATCTCCAAAGCCAAGATGACAACGGCCATATCGATTGAAGATGTTCGCCGAGAGGTGAAGATATTGAAAGCTCTATCTGGGCATAAGCATCTTGTAAAATTTTATGATGCATGTGAAGACGCCAATAATGTTTACATTATCATGGAACTATGTGAAGGTGGGGAACTTCTTGACAGAATTTTATCAAGAGGTGGAAAGTATGCAGAAGAAGATGCAAAAGTTATAGTTGTGCAAATTCTAAGTGTTGTTGCATTTTGTCATCTTCAAGGCGTTGTGCACCGTGACTTAAAGCCAGAGAACTTCCTTTTCGCTTCTAGAAATGAAGATGCTGATATGAAGCTTATTGATTTTGGTCTTTCTGACTTTATCAGACCAGATGAAAGACTTAATGATATTGTTGGGAGTGCATATTATGTTGCACCTGAAGTCCTTCATAGATCTTATAGCCTTGAGGGAGATATATGGAGCATTGGTGTTATCACCTATATCCTGCTTTGCGGAAGCCGGCCTTTCTGGGCACGAACAGAATCGGGAATTTTCCGTGCGGTGCTAAGAGCTGATCCTAGTTTTGATGATCCACCCTGGCCTTCTATGTCGCACGAGGCCAAGGACTTTGTAAAACGGCTTTTGAACAAGGACTACAGAAAGAGAATGACTGCTGTTCAGGCCTTATCTCACCCATGGTTGCGAGATGATAGTCGTCCTCTCCCTTTAGATATATTGATCTATAAATTAGTCAAATCATATCTGCATGCTACACCTTTCAAACGTGCAGCACTTAAGGCTCTTCAGAGAAATTCAACTGAAGCTATGATGGAGTCAAGGGTTGTTGATATTCTAAATGCAATGGAACCACTTGCATATAGGAAAATGGACTTTGAAGAATTTTGTGCTGCTGCAATTAGCACGCATCAATTGGAGGCCCTCGAAGGATGGGAGCAGATAGCATCTACTGCTTTTGAGCACTTCGAACGCGATGGCAACCGAGTCATATCGATTGAAGAACTGGCCAGAGAATTAAATCTGGGCACTTCTGCCTATTCAATCCTCAGAGATTGGATCAGAAACACAGATGGAAAACTTAATTTCATGGGATATACAAAATTTTTGCATGGTGTGACGATCCGTAGCTCCAACACCAGGCACCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010150 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032501 GO:0032502 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0090693 GO:0097305 GO:0097306 GO:0099402 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

Pkinase

Protein Analysis

574

Amino Acids

64.12

Weight (kDa)

8.8

Isoelectric Point (pI)

45.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	158 - 420	7.4e-71	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	159 - 411	1.9e-33	Protein tyrosine and serine/threonine kinase
Kdo	PF06293	201 - 311	2.2e-06	Lipopolysaccharide kinase (Kdo/WaaP) family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012710)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46700 AT2G46700 AT2G46700
fragaria_vesca	FvH4_7g17350 FvH4_7g17350
malus_domestica	MD01G1086500.v1.1 MD07G1156500.v1.1
prunus_persica	Prupe.2G194900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0360791
rosa_laevigata	RLG00000027786
rosa_multiflora	Rmu_sc0000692.1_g000010
rosa_roxburghii	Rroxscaffold_4G00295810
rosa_rugosa	Rorug01G0281500
rosa_samantha	Rh1AG293500 Rh1BG258100 Rh1CG276000 Rh1DG288400
rosa_wichuraiana	Rw1G025970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	191, 287
AccB1I	GGYRCC	2 cut(s)	155, 1716
AccI	GTMKAC	1 cut(s)	287
AccII	CGCG	4 cut(s)	83, 184, 320, 1548
AccIII	TCCGGA	1 cut(s)	397
AciI	CCGC	8 cut(s)	38, 184, 227, 248, 323, 364, 1071, 1118
AclWI	GGATC	5 cut(s)	187, 1127, 1142, 1640, 1690
AcoI	YGGCCR	4 cut(s)	212, 561, 597, 1581
AcsI	RAATTY	8 cut(s)	457, 671, 750, 807, 1107, 1369, 1457, 1676
AcuI	CTGAAG	3 cut(s)	1017, 1346, 1398
AcyI	GRCGYC	7 cut(s)	125, 156, 188, 284, 290, 350, 693
AdeI	CACNNNGTG	1 cut(s)	235
AfiI	CCNNNNNNNGG	4 cut(s)	253, 254, 397, 1713
AflII	CTTAAG	1 cut(s)	1352
AgsI	TTSAA	9 cut(s)	611, 640, 839, 1204, 1339, 1374, 1454, 1574, 1617
AjnI	CCWGG	4 cut(s)	209, 292, 1154, 1712
AluBI	AGCT	9 cut(s)	441, 467, 478, 544, 644, 913, 1130, 1382, 1704
AluI	AGCT	9 cut(s)	441, 467, 478, 544, 644, 913, 1130, 1382, 1704
Alw21I	GWGCWC	4 cut(s)	176, 546, 852, 1539
Alw26I	GTCTC	1 cut(s)	50
Alw44I	GTGCAC	1 cut(s)	848
AlwI	GGATC	5 cut(s)	187, 1127, 1142, 1640, 1690
AlwNI	CAGNNNCTG	1 cut(s)	430
Aor13HI	TCCGGA	1 cut(s)	397
AoxI	GGCC	9 cut(s)	212, 561, 597, 1079, 1157, 1176, 1239, 1494, 1581
ApaLI	GTGCAC	1 cut(s)	848
ApeKI	GCWGC	5 cut(s)	145, 250, 1346, 1466, 1469
ApoI	RAATTY	8 cut(s)	457, 671, 750, 807, 1107, 1369, 1457, 1676
Asp700I	GAANNNNTTC	3 cut(s)	365, 615, 1002
AspLEI	GCGC	4 cut(s)	83, 158, 184, 322
AspS9I	GGNCC	2 cut(s)	46, 1495
AsuC2I	CCSGG	3 cut(s)	61, 160, 255
AsuHPI	GGTGA	4 cut(s)	64, 640, 1045, 1241
AsuII	TTCGAA	1 cut(s)	1542
AvaII	GGWCC	1 cut(s)	46
BaeGI	GKGCMC	3 cut(s)	852, 1093, 1604
BalI	TGGCCA	1 cut(s)	1583
BanI	GGYRCC	2 cut(s)	155, 1716
BanII	GRGCYC	3 cut(s)	209, 392, 546
BauI	CACGAG	1 cut(s)	1172
BbsI	GAAGAC	1 cut(s)	696
Bbv12I	GWGCWC	4 cut(s)	176, 546, 852, 1539
BbvCI	CCTCAGC	1 cut(s)	198
BbvI	GCAGC	5 cut(s)	157, 237, 1358, 1453, 1456
BccI	CCATC	4 cut(s)	1381, 1500, 1544, 1640
BciT130I	CCWGG	4 cut(s)	211, 294, 1156, 1714
BcnI	CCSGG	3 cut(s)	61, 160, 255
BcoDI	GTCTC	1 cut(s)	50
BfaI	CTAG	2 cut(s)	888, 1137
BfmI	CTRYAG	1 cut(s)	1213
BfoI	RGCGCY	1 cut(s)	159
BfrI	CTTAAG	1 cut(s)	1352
BglII	AGATCT	1 cut(s)	1010
BisI	GCNGC	6 cut(s)	146, 251, 323, 1347, 1467, 1470
BlsI	GCNGC	6 cut(s)	147, 252, 324, 1348, 1468, 1471
Bme1390I	CCNGG	7 cut(s)	61, 160, 211, 255, 294, 1156, 1714
Bme18I	GGWCC	1 cut(s)	46
BmgT120I	GGNCC	2 cut(s)	46, 1495
BmiI	GGNNCC	3 cut(s)	157, 1425, 1718
BmrFI	CCNGG	7 cut(s)	61, 160, 211, 255, 294, 1156, 1714
BmsI	GCATC	6 cut(s)	670, 670, 775, 889, 1492, 1529
BoxI	GACNNNNGTC	3 cut(s)	68, 89, 110
BpiI	GAAGAC	1 cut(s)	696
Bpu10I	CCTNAGC	1 cut(s)	198
Bpu14I	TTCGAA	1 cut(s)	1542
BpuEI	CTTGAG	2 cut(s)	530, 1043
BpuMI	CCSGG	3 cut(s)	61, 160, 255
Bsa29I	ATCGAT	2 cut(s)	605, 1568
BsaHI	GRCGYC	7 cut(s)	125, 156, 188, 284, 290, 350, 693
BsaI	GGTCTC	1 cut(s)	50
BsaJI	CCNNGG	6 cut(s)	209, 254, 293, 1154, 1179, 1253
BsaWI	WCCGGW	3 cut(s)	48, 397, 521
BsaXI	ACNNNNNCTCC	2 cut(s)	1030, 1060
Bsc4I	CCNNNNNNNGG	4 cut(s)	253, 254, 397, 1713
Bse118I	RCCGGY	5 cut(s)	77, 178, 274, 331, 1077
Bse1I	ACTGG	1 cut(s)	1584
Bse3DI	GCAATG	1 cut(s)	1425
BseAI	TCCGGA	1 cut(s)	397
BseBI	CCWGG	4 cut(s)	211, 294, 1156, 1714
BseCI	ATCGAT	2 cut(s)	605, 1568
BseDI	CCNNGG	6 cut(s)	209, 254, 293, 1154, 1179, 1253
BseGI	GGATG	1 cut(s)	1511
BseLI	CCNNNNNNNGG	4 cut(s)	253, 254, 397, 1713
BseMI	GCAATG	1 cut(s)	1425
BseMII	CTCAG	2 cut(s)	212, 1636
BseNI	ACTGG	1 cut(s)	1584
BseRI	GAGGAG	4 cut(s)	152, 155, 325, 416
BseSI	GKGCMC	3 cut(s)	852, 1093, 1604
BseXI	GCAGC	5 cut(s)	157, 237, 1358, 1453, 1456
BsgI	GTGCAG	1 cut(s)	1365
Bsh1236I	CGCG	4 cut(s)	83, 184, 320, 1548
Bsh1285I	CGRYCG	1 cut(s)	90
BshFI	GGCC	9 cut(s)	214, 563, 599, 1081, 1159, 1178, 1241, 1496, 1583
BshNI	GGYRCC	2 cut(s)	155, 1716
BshVI	ATCGAT	2 cut(s)	605, 1568
BsiEI	CGRYCG	1 cut(s)	90
BsiHKAI	GWGCWC	4 cut(s)	176, 546, 852, 1539
BslFI	GGGAC	2 cut(s)	76, 204
BslI	CCNNNNNNNGG	4 cut(s)	253, 254, 397, 1713
BsmAI	GTCTC	1 cut(s)	50
BsmFI	GGGAC	2 cut(s)	76, 204
BsnI	GGCC	9 cut(s)	214, 563, 599, 1081, 1159, 1178, 1241, 1496, 1583
Bso31I	GGTCTC	1 cut(s)	50
Bsp119I	TTCGAA	1 cut(s)	1542
Bsp1286I	GDGCHC	8 cut(s)	176, 209, 392, 546, 852, 1093, 1539, 1604
Bsp13I	TCCGGA	1 cut(s)	397
Bsp143I	GATC	8 cut(s)	192, 570, 1010, 1132, 1147, 1296, 1632, 1695
Bsp19I	CCATGG	1 cut(s)	1253
BspACI	CCGC	8 cut(s)	38, 184, 227, 248, 323, 364, 1071, 1118
BspANI	GGCC	9 cut(s)	214, 563, 599, 1081, 1159, 1178, 1241, 1496, 1583
BspCNI	CTCAG	2 cut(s)	211, 1635
BspDI	ATCGAT	2 cut(s)	605, 1568
BspEI	TCCGGA	1 cut(s)	397
BspFNI	CGCG	4 cut(s)	83, 184, 320, 1548
BspLI	GGNNCC	3 cut(s)	157, 1425, 1718
BspPI	GGATC	5 cut(s)	187, 1127, 1142, 1640, 1690
BspQI	GCTCTTC	2 cut(s)	164, 1365
BspT104I	TTCGAA	1 cut(s)	1542
BspT107I	GGYRCC	2 cut(s)	155, 1716
BspTI	CTTAAG	1 cut(s)	1352
BspTNI	GGTCTC	1 cut(s)	50
BsrDI	GCAATG	1 cut(s)	1425
BsrFI	RCCGGY	5 cut(s)	77, 178, 274, 331, 1077
BsrI	ACTGG	1 cut(s)	1584
BssAI	RCCGGY	5 cut(s)	77, 178, 274, 331, 1077
BssECI	CCNNGG	6 cut(s)	209, 254, 293, 1154, 1179, 1253
BssMI	GATC	8 cut(s)	192, 570, 1010, 1132, 1147, 1296, 1632, 1695
BssNI	GRCGYC	7 cut(s)	125, 156, 188, 284, 290, 350, 693
BssSI	CACGAG	1 cut(s)	1172
BssT1I	CCWWGG	2 cut(s)	1179, 1253
Bst2BI	CACGAG	1 cut(s)	1172
Bst2UI	CCWGG	4 cut(s)	211, 294, 1156, 1714
Bst4CI	ACNGT	3 cut(s)	70, 91, 854
Bst6I	CTCTTC	2 cut(s)	164, 1365
BstACI	GRCGYC	7 cut(s)	125, 156, 188, 284, 290, 350, 693
BstAFI	CTTAAG	1 cut(s)	1352
BstAPI	GCANNNNNTGC	1 cut(s)	1526
BstBI	TTCGAA	1 cut(s)	1542
BstDEI	CTNAG	5 cut(s)	198, 468, 812, 1124, 1622
BstDSI	CCRYGG	1 cut(s)	1253
BstF5I	GGATG	1 cut(s)	1511
BstFNI	CGCG	4 cut(s)	83, 184, 320, 1548
BstH2I	RGCGCY	1 cut(s)	159
BstHHI	GCGC	4 cut(s)	83, 158, 184, 322
BstKTI	GATC	8 cut(s)	195, 573, 1013, 1135, 1150, 1299, 1635, 1698
BstMAI	GTCTC	1 cut(s)	50
BstMBI	GATC	8 cut(s)	192, 570, 1010, 1132, 1147, 1296, 1632, 1695
BstMCI	CGRYCG	1 cut(s)	90
BstNI	CCWGG	4 cut(s)	211, 294, 1156, 1714
BstNSI	RCATGY	2 cut(s)	687, 1328
BstPAI	GACNNNNGTC	3 cut(s)	68, 89, 110
BstSCI	CCNGG	7 cut(s)	59, 158, 209, 253, 292, 1154, 1712
BstSFI	CTRYAG	1 cut(s)	1213
BstSLI	GKGCMC	3 cut(s)	852, 1093, 1604
BstUI	CGCG	4 cut(s)	83, 184, 320, 1548
BstV1I	GCAGC	5 cut(s)	157, 237, 1358, 1453, 1456
BstV2I	GAAGAC	1 cut(s)	696
BstX2I	RGATCY	1 cut(s)	1010
BstYI	RGATCY	1 cut(s)	1010
Bsu15I	ATCGAT	2 cut(s)	605, 1568
BsuRI	GGCC	9 cut(s)	214, 563, 599, 1081, 1159, 1178, 1241, 1496, 1583
BsuTUI	ATCGAT	2 cut(s)	605, 1568
BtgI	CCRYGG	1 cut(s)	1253
BtgZI	GCGATG	1 cut(s)	1563
BtsCI	GGATG	1 cut(s)	1511
BtsI	GCAGTG	1 cut(s)	14
BtsIMutI	CAGTG	1 cut(s)	14
CaiI	CAGNNNCTG	1 cut(s)	430
CfoI	GCGC	4 cut(s)	83, 158, 184, 322
Cfr10I	RCCGGY	5 cut(s)	77, 178, 274, 331, 1077
Cfr13I	GGNCC	2 cut(s)	46, 1495
ClaI	ATCGAT	2 cut(s)	605, 1568
CseI	GACGC	4 cut(s)	133, 298, 339, 701
CviAII	CATG	6 cut(s)	684, 715, 1254, 1325, 1663, 1685
DdeI	CTNAG	5 cut(s)	198, 468, 812, 1124, 1622
DinI	GGCGCC	1 cut(s)	157
DpnI	GATC	8 cut(s)	194, 572, 1012, 1134, 1149, 1298, 1634, 1697
DpnII	GATC	8 cut(s)	192, 570, 1010, 1132, 1147, 1296, 1632, 1695
DraIII	CACNNNGTG	1 cut(s)	235
EaeI	YGGCCR	4 cut(s)	212, 561, 597, 1581
Eam1104I	CTCTTC	2 cut(s)	164, 1365
EarI	CTCTTC	2 cut(s)	164, 1365
EciI	GGCGGA	1 cut(s)	53
Ecl136II	GAGCTC	1 cut(s)	544
Eco130I	CCWWGG	2 cut(s)	1179, 1253
Eco147I	AGGCCT	1 cut(s)	1241
Eco24I	GRGCYC	3 cut(s)	209, 392, 546
Eco31I	GGTCTC	1 cut(s)	50
Eco47I	GGWCC	1 cut(s)	46
Eco53kI	GAGCTC	1 cut(s)	544
Eco57I	CTGAAG	3 cut(s)	1017, 1346, 1398
EcoICRI	GAGCTC	1 cut(s)	544
EcoO109I	RGGNCCY	1 cut(s)	1495
EcoRII	CCWGG	4 cut(s)	209, 292, 1154, 1712
EcoT14I	CCWWGG	2 cut(s)	1179, 1253
EcoT22I	ATGCAT	1 cut(s)	685
EcoT38I	GRGCYC	3 cut(s)	209, 392, 546
EgeI	GGCGCC	1 cut(s)	157
EheI	GGCGCC	1 cut(s)	157
ErhI	CCWWGG	2 cut(s)	1179, 1253
FaeI	CATG	6 cut(s)	687, 718, 1257, 1328, 1666, 1688
FalI	AAGNNNNNCTT	2 cut(s)	1066, 1098
FaqI	GGGAC	2 cut(s)	76, 204
FatI	CATG	6 cut(s)	683, 714, 1253, 1324, 1662, 1684
FauI	CCCGC	2 cut(s)	234, 255
FblI	GTMKAC	1 cut(s)	287
Fnu4HI	GCNGC	6 cut(s)	146, 251, 323, 1347, 1467, 1470
FokI	GGATG	1 cut(s)	1518
FriOI	GRGCYC	3 cut(s)	209, 392, 546
Fsp4HI	GCNGC	6 cut(s)	146, 251, 323, 1347, 1467, 1470
FspBI	CTAG	2 cut(s)	888, 1137
GlaI	GCGC	4 cut(s)	82, 157, 183, 321
GluI	GCNGC	6 cut(s)	146, 251, 323, 1347, 1467, 1470
HaeII	RGCGCY	1 cut(s)	159
HaeIII	GGCC	9 cut(s)	214, 563, 599, 1081, 1159, 1178, 1241, 1496, 1583
HgaI	GACGC	4 cut(s)	133, 298, 339, 701
HhaI	GCGC	4 cut(s)	83, 158, 184, 322
Hin1I	GRCGYC	7 cut(s)	125, 156, 188, 284, 290, 350, 693
Hin1II	CATG	6 cut(s)	687, 718, 1257, 1328, 1666, 1688
Hin6I	GCGC	4 cut(s)	81, 156, 182, 320
HinP1I	GCGC	4 cut(s)	81, 156, 182, 320
HincII	GTYRAC	1 cut(s)	288
HindII	GTYRAC	1 cut(s)	288
HindIII	AAGCTT	1 cut(s)	911
HinfI	GANTC	5 cut(s)	217, 394, 1100, 1391, 1560
HphI	GGTGA	4 cut(s)	64, 640, 1045, 1241
Hpy166II	GTNNAC	3 cut(s)	288, 706, 850
Hpy188I	TCNGA	7 cut(s)	30, 309, 934, 944, 1365, 1625, 1637
Hpy188III	TCNNGA	7 cut(s)	94, 103, 398, 743, 759, 888, 1104
Hpy8I	GTNNAC	3 cut(s)	288, 706, 850
HpyAV	CCTTC	5 cut(s)	376, 722, 1013, 1170, 1496
HpyCH4III	ACNGT	3 cut(s)	70, 91, 854
HpyCH4IV	ACGT	3 cut(s)	188, 284, 1342
HpyF3I	CTNAG	5 cut(s)	198, 468, 812, 1124, 1622
HpySE526I	ACGT	3 cut(s)	188, 284, 1342
Hsp92I	GRCGYC	7 cut(s)	125, 156, 188, 284, 290, 350, 693
Hsp92II	CATG	6 cut(s)	687, 718, 1257, 1328, 1666, 1688
HspAI	GCGC	4 cut(s)	81, 156, 182, 320
KasI	GGCGCC	1 cut(s)	155
Kpn2I	TCCGGA	1 cut(s)	397
KroI	GCCGGC	4 cut(s)	178, 274, 331, 1077
KroNI	GCCGGC	4 cut(s)	180, 276, 333, 1079
Kzo9I	GATC	8 cut(s)	192, 570, 1010, 1132, 1147, 1296, 1632, 1695
LguI	GCTCTTC	2 cut(s)	164, 1365
LmnI	GCTCC	9 cut(s)	142, 162, 403, 421, 464, 524, 1038, 1510, 1709
Lsp1109I	GCAGC	5 cut(s)	157, 237, 1358, 1453, 1456
LweI	GCATC	6 cut(s)	670, 670, 775, 889, 1492, 1529
MaeI	CTAG	2 cut(s)	888, 1137
MaeII	ACGT	3 cut(s)	188, 284, 1342
MaeIII	GTNAC	4 cut(s)	70, 91, 854, 1690
MalI	GATC	8 cut(s)	194, 572, 1012, 1134, 1149, 1298, 1634, 1697
MboI	GATC	8 cut(s)	192, 570, 1010, 1132, 1147, 1296, 1632, 1695
MfeI	CAATTG	1 cut(s)	1487
MflI	RGATCY	1 cut(s)	1010
MhlI	GDGCHC	8 cut(s)	176, 209, 392, 546, 852, 1093, 1539, 1604
MlsI	TGGCCA	1 cut(s)	1583
MluNI	TGGCCA	1 cut(s)	1583
Mly113I	GGCGCC	1 cut(s)	156
MlyI	GAGTC	3 cut(s)	226, 1400, 1569
MmeI	TCCRAC	1 cut(s)	53
Mox20I	TGGCCA	1 cut(s)	1583
Mph1103I	ATGCAT	1 cut(s)	685
MreI	CGCCGGCG	3 cut(s)	178, 274, 331
MroI	TCCGGA	1 cut(s)	397
MroNI	GCCGGC	4 cut(s)	178, 274, 331, 1077
MroXI	GAANNNNTTC	3 cut(s)	365, 615, 1002
MscI	TGGCCA	1 cut(s)	1583
MseI	TTAA	6 cut(s)	567, 860, 960, 1353, 1592, 1656
MslI	CAYNNNNRTG	1 cut(s)	713
Msp20I	TGGCCA	1 cut(s)	1583
MspA1I	CMGCKG	1 cut(s)	250
MspCI	CTTAAG	1 cut(s)	1352
MspR9I	CCNGG	7 cut(s)	61, 160, 211, 255, 294, 1156, 1714
MunI	CAATTG	1 cut(s)	1487
MvaI	CCWGG	4 cut(s)	211, 294, 1156, 1714
MvnI	CGCG	4 cut(s)	83, 184, 320, 1548
NaeI	GCCGGC	4 cut(s)	180, 276, 333, 1079
NarI	GGCGCC	1 cut(s)	156
NciI	CCSGG	3 cut(s)	61, 160, 255
NcoI	CCATGG	1 cut(s)	1253
NdeII	GATC	8 cut(s)	192, 570, 1010, 1132, 1147, 1296, 1632, 1695
NgoMIV	GCCGGC	4 cut(s)	178, 274, 331, 1077
NlaIII	CATG	6 cut(s)	687, 718, 1257, 1328, 1666, 1688
NlaIV	GGNNCC	3 cut(s)	157, 1425, 1718
NmeAIII	GCCGAG	3 cut(s)	228, 240, 647
NmuCI	GTSAC	4 cut(s)	70, 91, 854, 1690
NsiI	ATGCAT	1 cut(s)	685
NspI	RCATGY	2 cut(s)	687, 1328
NspV	TTCGAA	1 cut(s)	1542
PaeI	GCATGC	1 cut(s)	1328
PceI	AGGCCT	1 cut(s)	1241
PciSI	GCTCTTC	2 cut(s)	164, 1365
PcsI	WCGNNNNNNNCGW	3 cut(s)	92, 101, 602
PdiI	GCCGGC	4 cut(s)	180, 276, 333, 1079
PdmI	GAANNNNTTC	3 cut(s)	365, 615, 1002
PfeI	GAWTC	2 cut(s)	394, 1100
PflFI	GACNNNGTC	1 cut(s)	127
PkrI	GCNGC	6 cut(s)	147, 252, 324, 1348, 1468, 1471
PleI	GAGTC	3 cut(s)	225, 1399, 1568
PluTI	GGCGCC	1 cut(s)	159
PpsI	GAGTC	3 cut(s)	225, 1399, 1568
PshAI	GACNNNNGTC	3 cut(s)	68, 89, 110
Psp124BI	GAGCTC	1 cut(s)	546
Psp6I	CCWGG	4 cut(s)	209, 292, 1154, 1712
PspGI	CCWGG	4 cut(s)	209, 292, 1154, 1712
PspN4I	GGNNCC	3 cut(s)	157, 1425, 1718
PspPI	GGNCC	2 cut(s)	46, 1495
PsrI	GAACNNNNNNTAC	2 cut(s)	1196, 1228
PstNI	CAGNNNCTG	1 cut(s)	430
PsuI	RGATCY	1 cut(s)	1010
PsyI	GACNNNGTC	1 cut(s)	127
RseI	CAYNNNNRTG	1 cut(s)	713
SacI	GAGCTC	1 cut(s)	546
SalI	GTCGAC	1 cut(s)	286
SapI	GCTCTTC	2 cut(s)	164, 1365
SaqAI	TTAA	6 cut(s)	567, 860, 960, 1353, 1592, 1656
SatI	GCNGC	6 cut(s)	146, 251, 323, 1347, 1467, 1470
Sau3AI	GATC	8 cut(s)	192, 570, 1010, 1132, 1147, 1296, 1632, 1695
Sau96I	GGNCC	2 cut(s)	46, 1495
SchI	GAGTC	3 cut(s)	226, 1400, 1569
ScrFI	CCNGG	7 cut(s)	61, 160, 211, 255, 294, 1156, 1714
SduI	GDGCHC	8 cut(s)	176, 209, 392, 546, 852, 1093, 1539, 1604
SfaNI	GCATC	6 cut(s)	670, 670, 775, 889, 1492, 1529
SfcI	CTRYAG	1 cut(s)	1213
SfoI	GGCGCC	1 cut(s)	157
SfuI	TTCGAA	1 cut(s)	1542
SgrAI	CRCCGGYG	4 cut(s)	77, 178, 274, 331
SgrDI	CGTCGACG	1 cut(s)	286
SinI	GGWCC	1 cut(s)	46
SmiMI	CAYNNNNRTG	1 cut(s)	713
SmlI	CTYRAG	3 cut(s)	545, 1022, 1352
SmoI	CTYRAG	3 cut(s)	545, 1022, 1352
SphI	GCATGC	1 cut(s)	1328
SseBI	AGGCCT	1 cut(s)	1241
SsiI	CCGC	8 cut(s)	38, 184, 227, 248, 323, 364, 1071, 1118
SspDI	GGCGCC	1 cut(s)	155
SspMI	CTAG	2 cut(s)	888, 1137
SstI	GAGCTC	1 cut(s)	546
StuI	AGGCCT	1 cut(s)	1241
StyD4I	CCNGG	7 cut(s)	59, 158, 209, 253, 292, 1154, 1712
StyI	CCWWGG	2 cut(s)	1179, 1253
TaaI	ACNGT	3 cut(s)	70, 91, 854
TaiI	ACGT	3 cut(s)	191, 287, 1345
TaqI	TCGA	7 cut(s)	104, 191, 287, 605, 1500, 1542, 1568
TauI	GCSGC	1 cut(s)	325
TfiI	GAWTC	2 cut(s)	394, 1100
Tru1I	TTAA	6 cut(s)	567, 860, 960, 1353, 1592, 1656
Tru9I	TTAA	6 cut(s)	567, 860, 960, 1353, 1592, 1656
TscAI	CASTG	1 cut(s)	14
TseFI	GTSAC	4 cut(s)	70, 91, 854, 1690
TseI	GCWGC	5 cut(s)	145, 250, 1346, 1466, 1469
Tsp45I	GTSAC	4 cut(s)	70, 91, 854, 1690
TspDTI	ATGAA	5 cut(s)	909, 923, 966, 995, 1651
TspGWI	ACGGA	4 cut(s)	68, 100, 1103, 1688
TspRI	CASTG	1 cut(s)	14
Tth111I	GACNNNGTC	1 cut(s)	127
Vha464I	CTTAAG	1 cut(s)	1352
VneI	GTGCAC	1 cut(s)	848
VpaK11BI	GGWCC	1 cut(s)	46
XapI	RAATTY	8 cut(s)	457, 671, 750, 807, 1107, 1369, 1457, 1676
XbaI	TCTAGA	1 cut(s)	887
XceI	RCATGY	2 cut(s)	687, 1328
XmiI	GTMKAC	1 cut(s)	287
XmnI	GAANNNNTTC	3 cut(s)	365, 615, 1002
XspI	CTAG	2 cut(s)	888, 1137
ZraI	GACGTC	2 cut(s)	189, 285
Zsp2I	ATGCAT	1 cut(s)	685

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.