MD01G1098700.v1.1

Metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0019772
Physical Location & Seq
Reverse (-)
21244933 .. 21245895
963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1098700.v1.1.491

Sequence Viewer

Length: 963 bp
ATGCCAGCAATGGCCTCGAAAACCGCCTCGGTTTGCGTAGTCTTTTGCTTCTTGATCTTTGTGTTGCTGTCTCAGAGAACTCTTGCAAAACCTACTGATCCCCATGGTCATCAGCATGATTCCTTCAAGTTCATCCAACACTTGGAGGGATGCCACAAGGGTCAAAACGTCAGTGGGTTGCAAGAGCTCAAAAAATACTTCACCAAATTCGGCTACTTGAATTATGATCACTCAAAACACGCCAACGACGATGAGTTTGATGACCTTCTAGAGTCCGCCATCAAGTCATACCAAAAAAGCTTCCACTTAAATGTAACCGGAAGTCTAGACACTACCACGGTGAAACGAATGATGGTGCCAAGATGTGGTGTGCCAGATGTTGTCAATGGCACGCGAAAGGGAACCAAAAAGCACAACCGCAAGCTCAAGTCCATCCATGGAGTTTCTCATTATGAGTTCTTCTTCCCTGGTCCGCTAAGATGGCCACCTACGAAAACCCATTTGAGATATCGCTTTGCTTCGAGTGCAAGCCAAGTCCCCGGCACAGAGAATGTGAGGTCTATATGTGCACAAGCATTTCAGAGATGGGCACAAGTGACCAGTTTCACATTTGAGGAAGTGCCTGCTACTTCATCAGCTGACATTACCATTGGCTTTCACCGGGGCGCCCATGGAGATACGGCCCCATTTGACGGCTTCAAAGGGGTTTTAGCACACGCGAATCCGCCTACAGGTGGTAATTTCCATTTTGATGCAGATGAGAAGTGGAGTGCCAATCCAGGACCCGATGAGCTAGACTTGGAATCCGTCGCTGTGCACGAAATCGGTCATCTTCTAGGACTTGATCACGATCTTGACCTCCCCGATGCGATCATGTATCCCGAATTTTCTTATGGGATTGTAAAACGTGATTTGACTAGGGATGACATTGATGGAATACGTGCTTTGTATGGTTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

321

Amino Acids

35.76

Weight (kDa)

6.67

Isoelectric Point (pI)

29.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 59 - 117 1.4e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 161 - 318 3.7e-46 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019772)

Species Orthologous Gene IDs
malus_domestica MD01G1098700.v1.1 MD07G1165400.v1.1
prunus_persica Prupe.2G204200_v2.0.a1 Prupe.2G204300_v2.0.a1 Prupe.2G204500_v2.0.a1
pyrus_communis pycom01g12590 pycom07g16090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 355, 665
AccB7I CCANNNNNTGG 2 cut(s) 142, 365
AccII CGCG 2 cut(s) 394, 719
AciI CCGC 5 cut(s) 24, 276, 418, 473, 725
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 2 cut(s) 206, 884
AcyI GRCGYC 1 cut(s) 666
AfiI CCNNNNNNNGG 5 cut(s) 142, 365, 692, 731, 734
AgsI TTSAA 3 cut(s) 127, 220, 700
AjnI CCWGG 2 cut(s) 466, 778
AluBI AGCT 5 cut(s) 187, 300, 424, 638, 793
AluI AGCT 5 cut(s) 187, 300, 424, 638, 793
Alw21I GWGCWC 3 cut(s) 189, 571, 819
Alw26I GTCTC 1 cut(s) 75
Alw44I GTGCAC 2 cut(s) 567, 815
AlwI GGATC 1 cut(s) 92
AoxI GGCC 3 cut(s) 12, 482, 681
ApaLI GTGCAC 2 cut(s) 567, 815
ApoI RAATTY 2 cut(s) 206, 884
ArsI GACNNNNNNTTYG 2 cut(s) 239, 271
AspLEI GCGC 1 cut(s) 668
AspS9I GGNCC 3 cut(s) 470, 682, 782
AsuC2I CCSGG 2 cut(s) 540, 662
AsuHPI GGTGA 3 cut(s) 193, 352, 650
AvaII GGWCC 2 cut(s) 470, 782
BaeGI GKGCMC 3 cut(s) 571, 592, 819
BalI TGGCCA 1 cut(s) 484
BanI GGYRCC 2 cut(s) 355, 665
BanII GRGCYC 1 cut(s) 189
Bbv12I GWGCWC 3 cut(s) 189, 571, 819
BccI CCATC 6 cut(s) 287, 346, 440, 474, 579, 926
BceAI ACGGC 2 cut(s) 696, 709
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 2 cut(s) 468, 780
BciVI GTATCC 1 cut(s) 888
BclI TGATCA 2 cut(s) 226, 844
BcnI CCSGG 2 cut(s) 540, 662
BcoDI GTCTC 1 cut(s) 75
BfaI CTAG 5 cut(s) 269, 326, 794, 836, 918
BfmI CTRYAG 1 cut(s) 729
BfoI RGCGCY 1 cut(s) 669
BfuI GTATCC 1 cut(s) 888
Bme1390I CCNGG 4 cut(s) 468, 540, 662, 780
Bme18I GGWCC 2 cut(s) 470, 782
BmgT120I GGNCC 3 cut(s) 470, 682, 782
BmiI GGNNCC 5 cut(s) 357, 403, 667, 684, 784
BmrFI CCNGG 4 cut(s) 468, 540, 662, 780
BmsI GCATC 3 cut(s) 140, 742, 856
BpuEI CTTGAG 1 cut(s) 410
BpuMI CCSGG 2 cut(s) 540, 662
BsaAI YACGTR 1 cut(s) 941
BsaBI GATNNNNATC 1 cut(s) 849
BsaHI GRCGYC 1 cut(s) 666
BsaJI CCNNGG 8 cut(s) 27, 103, 336, 436, 466, 538, 661, 670
BsaWI WCCGGW 1 cut(s) 317
Bsc4I CCNNNNNNNGG 5 cut(s) 142, 365, 692, 731, 734
Bse1I ACTGG 1 cut(s) 600
Bse3DI GCAATG 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 849
BseBI CCWGG 2 cut(s) 468, 780
BseDI CCNNGG 8 cut(s) 27, 103, 336, 436, 466, 538, 661, 670
BseGI GGATG 4 cut(s) 132, 155, 432, 928
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 5 cut(s) 142, 365, 692, 731, 734
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 1 cut(s) 600
BseSI GKGCMC 3 cut(s) 571, 592, 819
Bsh1236I CGCG 2 cut(s) 394, 719
BshFI GGCC 3 cut(s) 14, 484, 683
BshNI GGYRCC 2 cut(s) 355, 665
BsiHKAI GWGCWC 3 cut(s) 189, 571, 819
BsiSI CCGG 3 cut(s) 318, 540, 661
BslFI GGGAC 1 cut(s) 521
BslI CCNNNNNNNGG 5 cut(s) 142, 365, 692, 731, 734
BsmAI GTCTC 1 cut(s) 75
BsmFI GGGAC 1 cut(s) 521
BsnI GGCC 3 cut(s) 14, 484, 683
Bsp1286I GDGCHC 4 cut(s) 189, 571, 592, 819
Bsp143I GATC 6 cut(s) 54, 97, 226, 844, 850, 870
Bsp19I CCATGG 3 cut(s) 103, 436, 670
BspACI CCGC 5 cut(s) 24, 276, 418, 473, 725
BspANI GGCC 3 cut(s) 14, 484, 683
BspCNI CTCAG 1 cut(s) 85
BspFNI CGCG 2 cut(s) 394, 719
BspLI GGNNCC 5 cut(s) 357, 403, 667, 684, 784
BspPI GGATC 1 cut(s) 92
BspT107I GGYRCC 2 cut(s) 355, 665
BsrDI GCAATG 1 cut(s) 15
BsrI ACTGG 1 cut(s) 600
BssECI CCNNGG 8 cut(s) 27, 103, 336, 436, 466, 538, 661, 670
BssMI GATC 6 cut(s) 54, 97, 226, 844, 850, 870
BssNI GRCGYC 1 cut(s) 666
BssT1I CCWWGG 3 cut(s) 103, 436, 670
Bst2UI CCWGG 2 cut(s) 468, 780
Bst4CI ACNGT 2 cut(s) 340, 960
BstACI GRCGYC 1 cut(s) 666
BstBAI YACGTR 1 cut(s) 941
BstC8I GCNNGC 5 cut(s) 6, 392, 422, 529, 624
BstDEI CTNAG 2 cut(s) 72, 476
BstDSI CCRYGG 4 cut(s) 103, 336, 436, 670
BstF5I GGATG 4 cut(s) 132, 155, 432, 928
BstFNI CGCG 2 cut(s) 394, 719
BstH2I RGCGCY 1 cut(s) 669
BstHHI GCGC 1 cut(s) 668
BstKTI GATC 6 cut(s) 57, 100, 229, 847, 853, 873
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 6 cut(s) 54, 97, 226, 844, 850, 870
BstMWI GCNNNNNNNGC 2 cut(s) 481, 524
BstNI CCWGG 2 cut(s) 468, 780
BstSCI CCNGG 4 cut(s) 466, 538, 660, 778
BstSFI CTRYAG 1 cut(s) 729
BstSLI GKGCMC 3 cut(s) 571, 592, 819
BstUI CGCG 2 cut(s) 394, 719
BsuI GTATCC 1 cut(s) 888
BsuRI GGCC 3 cut(s) 14, 484, 683
BtgI CCRYGG 4 cut(s) 103, 336, 436, 670
BtsCI GGATG 4 cut(s) 132, 155, 432, 928
BtsIMutI CAGTG 1 cut(s) 178
Cac8I GCNNGC 5 cut(s) 6, 392, 422, 529, 624
CfoI GCGC 1 cut(s) 668
Cfr13I GGNCC 3 cut(s) 470, 682, 782
CviAII CATG 5 cut(s) 104, 116, 437, 671, 874
DdeI CTNAG 2 cut(s) 72, 476
DinI GGCGCC 1 cut(s) 667
DpnI GATC 6 cut(s) 56, 99, 228, 846, 852, 872
DpnII GATC 6 cut(s) 54, 97, 226, 844, 850, 870
EaeI YGGCCR 1 cut(s) 482
EciI GGCGGA 2 cut(s) 265, 714
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 3 cut(s) 103, 436, 670
Eco24I GRGCYC 1 cut(s) 189
Eco32I GATATC 1 cut(s) 509
Eco47I GGWCC 2 cut(s) 470, 782
Eco53kI GAGCTC 1 cut(s) 187
EcoICRI GAGCTC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 782
EcoRII CCWGG 2 cut(s) 466, 778
EcoRV GATATC 1 cut(s) 509
EcoT14I CCWWGG 3 cut(s) 103, 436, 670
EcoT38I GRGCYC 1 cut(s) 189
EgeI GGCGCC 1 cut(s) 667
EheI GGCGCC 1 cut(s) 667
ErhI CCWWGG 3 cut(s) 103, 436, 670
FaeI CATG 5 cut(s) 107, 119, 440, 674, 877
FaqI GGGAC 1 cut(s) 521
FatI CATG 5 cut(s) 103, 115, 436, 670, 873
FbaI TGATCA 2 cut(s) 226, 844
FokI GGATG 4 cut(s) 119, 162, 419, 935
FriOI GRGCYC 1 cut(s) 189
FspBI CTAG 5 cut(s) 269, 326, 794, 836, 918
GlaI GCGC 1 cut(s) 667
HaeII RGCGCY 1 cut(s) 669
HaeIII GGCC 3 cut(s) 14, 484, 683
HapII CCGG 3 cut(s) 318, 540, 661
HhaI GCGC 1 cut(s) 668
Hin1I GRCGYC 1 cut(s) 666
Hin1II CATG 5 cut(s) 107, 119, 440, 674, 877
Hin6I GCGC 1 cut(s) 666
HinP1I GCGC 1 cut(s) 666
HindIII AAGCTT 1 cut(s) 298
HinfI GANTC 4 cut(s) 119, 272, 721, 803
HpaII CCGG 3 cut(s) 318, 540, 661
HphI GGTGA 3 cut(s) 193, 352, 650
Hpy166II GTNNAC 3 cut(s) 569, 817, 956
Hpy188I TCNGA 2 cut(s) 75, 582
Hpy188III TCNNGA 6 cut(s) 52, 269, 326, 848, 854, 881
Hpy8I GTNNAC 3 cut(s) 569, 817, 956
Hpy99I CGWCG 2 cut(s) 251, 812
HpyAV CCTTC 2 cut(s) 133, 275
HpyCH4III ACNGT 2 cut(s) 340, 960
HpyCH4IV ACGT 3 cut(s) 168, 907, 940
HpyCH4V TGCA 6 cut(s) 86, 181, 527, 569, 755, 817
HpyF10VI GCNNNNNNNGC 2 cut(s) 481, 524
HpyF3I CTNAG 2 cut(s) 72, 476
HpySE526I ACGT 3 cut(s) 168, 907, 940
Hsp92I GRCGYC 1 cut(s) 666
Hsp92II CATG 5 cut(s) 107, 119, 440, 674, 877
HspAI GCGC 1 cut(s) 666
KasI GGCGCC 1 cut(s) 665
Ksp22I TGATCA 2 cut(s) 226, 844
Kzo9I GATC 6 cut(s) 54, 97, 226, 844, 850, 870
LweI GCATC 3 cut(s) 140, 742, 856
MaeI CTAG 5 cut(s) 269, 326, 794, 836, 918
MaeII ACGT 3 cut(s) 168, 907, 940
MaeIII GTNAC 2 cut(s) 313, 595
MalI GATC 6 cut(s) 56, 99, 228, 846, 852, 872
MboI GATC 6 cut(s) 54, 97, 226, 844, 850, 870
MboII GAAGA 3 cut(s) 451, 454, 824
MhlI GDGCHC 4 cut(s) 189, 571, 592, 819
MlsI TGGCCA 1 cut(s) 484
MluCI AATT 4 cut(s) 206, 220, 739, 884
MluNI TGGCCA 1 cut(s) 484
Mly113I GGCGCC 1 cut(s) 666
MlyI GAGTC 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 6 cut(s) 25, 37, 139, 549, 607, 869
Mox20I TGGCCA 1 cut(s) 484
MscI TGGCCA 1 cut(s) 484
MseI TTAA 1 cut(s) 308
MslI CAYNNNNRTG 3 cut(s) 114, 309, 750
Msp20I TGGCCA 1 cut(s) 484
MspA1I CMGCKG 1 cut(s) 638
MspI CCGG 3 cut(s) 318, 540, 661
MspR9I CCNGG 4 cut(s) 468, 540, 662, 780
MvaI CCWGG 2 cut(s) 468, 780
MvnI CGCG 2 cut(s) 394, 719
MwoI GCNNNNNNNGC 2 cut(s) 481, 524
NarI GGCGCC 1 cut(s) 666
NciI CCSGG 2 cut(s) 540, 662
NcoI CCATGG 3 cut(s) 103, 436, 670
NdeII GATC 6 cut(s) 54, 97, 226, 844, 850, 870
NlaIII CATG 5 cut(s) 107, 119, 440, 674, 877
NlaIV GGNNCC 5 cut(s) 357, 403, 667, 684, 784
NmuCI GTSAC 1 cut(s) 595
PcsI WCGNNNNNNNCGW 2 cut(s) 246, 816
PfeI GAWTC 3 cut(s) 119, 721, 803
PflMI CCANNNNNTGG 2 cut(s) 142, 365
PfoI TCCNGGA 1 cut(s) 778
PleI GAGTC 1 cut(s) 280
PluTI GGCGCC 1 cut(s) 669
PpsI GAGTC 1 cut(s) 280
Ppu21I YACGTR 1 cut(s) 941
PpuMI RGGWCCY 1 cut(s) 782
Psp124BI GAGCTC 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 782
Psp6I CCWGG 2 cut(s) 466, 778
PspGI CCWGG 2 cut(s) 466, 778
PspN4I GGNNCC 5 cut(s) 357, 403, 667, 684, 784
PspPI GGNCC 3 cut(s) 470, 682, 782
PspPPI RGGWCCY 1 cut(s) 782
PvuII CAGCTG 1 cut(s) 638
RseI CAYNNNNRTG 3 cut(s) 114, 309, 750
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 308
Sau3AI GATC 6 cut(s) 54, 97, 226, 844, 850, 870
Sau96I GGNCC 3 cut(s) 470, 682, 782
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 4 cut(s) 468, 540, 662, 780
SduI GDGCHC 4 cut(s) 189, 571, 592, 819
SfaNI GCATC 3 cut(s) 140, 742, 856
SfcI CTRYAG 1 cut(s) 729
SfoI GGCGCC 1 cut(s) 667
SinI GGWCC 2 cut(s) 470, 782
SmiMI CAYNNNNRTG 3 cut(s) 114, 309, 750
SmlI CTYRAG 1 cut(s) 425
SmoI CTYRAG 1 cut(s) 425
Sse9I AATT 4 cut(s) 206, 220, 739, 884
SsiI CCGC 5 cut(s) 24, 276, 418, 473, 725
SspDI GGCGCC 1 cut(s) 665
SspMI CTAG 5 cut(s) 269, 326, 794, 836, 918
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 4 cut(s) 466, 538, 660, 778
StyI CCWWGG 3 cut(s) 103, 436, 670
TaaI ACNGT 2 cut(s) 340, 960
TaiI ACGT 3 cut(s) 171, 910, 943
TaqI TCGA 2 cut(s) 17, 521
TaqII GACCGA 1 cut(s) 815
TasI AATT 4 cut(s) 206, 220, 739, 884
TfiI GAWTC 3 cut(s) 119, 721, 803
Tru1I TTAA 1 cut(s) 308
Tru9I TTAA 1 cut(s) 308
TscAI CASTG 1 cut(s) 178
TseFI GTSAC 1 cut(s) 595
Tsp45I GTSAC 1 cut(s) 595
TspDTI ATGAA 2 cut(s) 121, 621
TspGWI ACGGA 1 cut(s) 796
TspRI CASTG 1 cut(s) 178
Van91I CCANNNNNTGG 2 cut(s) 142, 365
VneI GTGCAC 2 cut(s) 567, 815
VpaK11BI GGWCC 2 cut(s) 470, 782
XapI RAATTY 2 cut(s) 206, 884
XbaI TCTAGA 2 cut(s) 268, 325
XspI CTAG 5 cut(s) 269, 326, 794, 836, 918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.