pycom07g16090

Metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0019772
Physical Location & Seq
Reverse (-)
18557109 .. 18557723
615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g16090.1

Sequence Viewer

Length: 615 bp
ATGATGGTGCCCAGATGTGGTGTGCCAGATGTTGTAAACGGCACGCGAAAGGGAAGCAAAAAGCACAACCGCAAACTCAAGTCCATCCATGGAGTTTCTCATTATGAGTTCTTCTTCCCTGGACCTCGAAGATGGCCATCTACACAAACCCATCTGAGATATCGCTTTGCTTCGAGTGCAAGGCAAGTCCCCGGCACAGAGAATGTAAGGTCTATATGTGCACAAGCATTTCAGAGATGGGCACAAGTGACCACTTTTACATTTGAGGAAGTTCCTTCTACTTCAGCGGCTGACATTACCATTGGCTTCCACCGTGGCAACCATGGAGATGGATCCTCATTTGACGGCTTCAAAGGGACTTACGCGCATGCGAATCCGCCTAAAGGTGGAAACTTCCATTTTGATGCAGATGAGACATGGAGTGCCAATCCAGGGCCGAATGAGGTAGACTTGGAATCAGTTGCTGTACACGAGATAGGCCATCTTCTGGGGCTTAATCACAATCCTGATCTCCCCGATGCAATCATGTATCCCTACTTTGAATATGGGATGGTGAAACGTGATCTGAATAGGGATGACATTGATGGTATACGTACTTTGTATGGTTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

205

Amino Acids

22.86

Weight (kDa)

7.19

Isoelectric Point (pI)

32.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M10 PF00413 45 - 202 2.3e-47 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019772)

Species Orthologous Gene IDs
malus_domestica MD01G1098700.v1.1 MD07G1165400.v1.1
prunus_persica Prupe.2G204200_v2.0.a1 Prupe.2G204300_v2.0.a1 Prupe.2G204500_v2.0.a1
pyrus_communis pycom01g12590 pycom07g16090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccB7I CCANNNNNTGG 1 cut(s) 487
AccI GTMKAC 2 cut(s) 447, 589
AccII CGCG 2 cut(s) 46, 365
AciI CCGC 3 cut(s) 70, 287, 377
AclWI GGATC 2 cut(s) 327, 340
AcoI YGGCCR 1 cut(s) 134
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 2 cut(s) 468, 595
AfiI CCNNNNNNNGG 5 cut(s) 17, 383, 386, 432, 487
AgsI TTSAA 2 cut(s) 352, 542
AjnI CCWGG 2 cut(s) 118, 430
Alw21I GWGCWC 1 cut(s) 223
Alw26I GTCTC 1 cut(s) 407
Alw44I GTGCAC 1 cut(s) 219
AlwI GGATC 2 cut(s) 327, 340
AlwNI CAGNNNCTG 2 cut(s) 290, 464
AoxI GGCC 3 cut(s) 134, 434, 478
ApaLI GTGCAC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 2 cut(s) 122, 434
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 1 cut(s) 565
AvaII GGWCC 1 cut(s) 122
BaeGI GKGCMC 3 cut(s) 12, 223, 244
BalI TGGCCA 1 cut(s) 136
BamHI GGATCC 1 cut(s) 332
BanI GGYRCC 1 cut(s) 7
BauI CACGAG 1 cut(s) 470
Bbv12I GWGCWC 1 cut(s) 223
BccI CCATC 9 cut(s) 92, 126, 145, 159, 231, 323, 489, 544, 578
BceAI ACGGC 2 cut(s) 55, 361
BciT130I CCWGG 2 cut(s) 120, 432
BciVI GTATCC 1 cut(s) 540
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 407
BfuI GTATCC 1 cut(s) 540
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 3 cut(s) 120, 192, 432
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 2 cut(s) 122, 434
BmiI GGNNCC 2 cut(s) 9, 334
BmrFI CCNGG 3 cut(s) 120, 192, 432
BmsI GCATC 2 cut(s) 394, 508
BpuEI CTTGAG 1 cut(s) 62
BpuMI CCSGG 1 cut(s) 192
BsaAI YACGTR 1 cut(s) 593
BsaBI GATNNNNATC 1 cut(s) 136
BsaJI CCNNGG 6 cut(s) 88, 118, 190, 313, 322, 431
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 383, 386, 432, 487
Bse8I GATNNNNATC 1 cut(s) 136
BseBI CCWGG 2 cut(s) 120, 432
BseDI CCNNGG 6 cut(s) 88, 118, 190, 313, 322, 431
BseGI GGATG 3 cut(s) 84, 555, 580
BseJI GATNNNNATC 1 cut(s) 136
BseLI CCNNNNNNNGG 5 cut(s) 17, 383, 386, 432, 487
BseMII CTCAG 1 cut(s) 146
BseSI GKGCMC 3 cut(s) 12, 223, 244
Bsh1236I CGCG 2 cut(s) 46, 365
BshFI GGCC 3 cut(s) 136, 436, 480
BshNI GGYRCC 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 223
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 173, 370
BslI CCNNNNNNNGG 5 cut(s) 17, 383, 386, 432, 487
BsmAI GTCTC 1 cut(s) 407
BsmFI GGGAC 2 cut(s) 173, 370
BsnI GGCC 3 cut(s) 136, 436, 480
Bsp1286I GDGCHC 3 cut(s) 12, 223, 244
Bsp1407I TGTACA 1 cut(s) 466
Bsp143I GATC 3 cut(s) 332, 508, 562
Bsp19I CCATGG 2 cut(s) 88, 322
BspACI CCGC 3 cut(s) 70, 287, 377
BspANI GGCC 3 cut(s) 136, 436, 480
BspCNI CTCAG 1 cut(s) 147
BspFNI CGCG 2 cut(s) 46, 365
BspLI GGNNCC 2 cut(s) 9, 334
BspPI GGATC 2 cut(s) 327, 340
BspT107I GGYRCC 1 cut(s) 7
BsrGI TGTACA 1 cut(s) 466
BssECI CCNNGG 6 cut(s) 88, 118, 190, 313, 322, 431
BssMI GATC 3 cut(s) 332, 508, 562
BssNAI GTATAC 1 cut(s) 590
BssSI CACGAG 1 cut(s) 470
BssT1I CCWWGG 2 cut(s) 88, 322
Bst1107I GTATAC 1 cut(s) 590
Bst2BI CACGAG 1 cut(s) 470
Bst2UI CCWGG 2 cut(s) 120, 432
Bst4CI ACNGT 2 cut(s) 314, 612
BstAUI TGTACA 1 cut(s) 466
BstBAI YACGTR 1 cut(s) 593
BstC8I GCNNGC 2 cut(s) 44, 369
BstDEI CTNAG 1 cut(s) 155
BstDSI CCRYGG 3 cut(s) 88, 313, 322
BstF5I GGATG 3 cut(s) 84, 555, 580
BstFNI CGCG 2 cut(s) 46, 365
BstHHI GCGC 1 cut(s) 367
BstKTI GATC 3 cut(s) 335, 511, 565
BstMAI GTCTC 1 cut(s) 407
BstMBI GATC 3 cut(s) 332, 508, 562
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNI CCWGG 2 cut(s) 120, 432
BstNSI RCATGY 1 cut(s) 371
BstSCI CCNGG 3 cut(s) 118, 190, 430
BstSLI GKGCMC 3 cut(s) 12, 223, 244
BstSNI TACGTA 1 cut(s) 593
BstUI CGCG 2 cut(s) 46, 365
BstX2I RGATCY 1 cut(s) 332
BstXI CCANNNNNNTGG 1 cut(s) 329
BstYI RGATCY 1 cut(s) 332
BstZ17I GTATAC 1 cut(s) 590
BsuI GTATCC 1 cut(s) 540
BsuRI GGCC 3 cut(s) 136, 436, 480
BtgI CCRYGG 3 cut(s) 88, 313, 322
BtsCI GGATG 3 cut(s) 84, 555, 580
Cac8I GCNNGC 2 cut(s) 44, 369
CaiI CAGNNNCTG 2 cut(s) 290, 464
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 2 cut(s) 122, 434
Csp6I GTAC 2 cut(s) 467, 594
CviAII CATG 5 cut(s) 89, 323, 368, 417, 526
CviJI RGCY 7 cut(s) 136, 290, 306, 348, 436, 480, 493
CviKI_1 RGCY 7 cut(s) 136, 290, 306, 348, 436, 480, 493
CviQI GTAC 2 cut(s) 467, 594
DdeI CTNAG 1 cut(s) 155
DpnI GATC 3 cut(s) 334, 510, 564
DpnII GATC 3 cut(s) 332, 508, 562
EaeI YGGCCR 1 cut(s) 134
EciI GGCGGA 1 cut(s) 366
Eco105I TACGTA 1 cut(s) 593
Eco130I CCWWGG 2 cut(s) 88, 322
Eco32I GATATC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 122
Eco57I CTGAAG 1 cut(s) 267
EcoRII CCWGG 2 cut(s) 118, 430
EcoRV GATATC 1 cut(s) 161
EcoT14I CCWWGG 2 cut(s) 88, 322
ErhI CCWWGG 2 cut(s) 88, 322
FaeI CATG 5 cut(s) 92, 326, 371, 420, 529
FaqI GGGAC 2 cut(s) 173, 370
FatI CATG 5 cut(s) 88, 322, 367, 416, 525
FblI GTMKAC 2 cut(s) 447, 589
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 3 cut(s) 71, 562, 587
Fsp4HI GCNGC 1 cut(s) 288
GlaI GCGC 1 cut(s) 366
GluI GCNGC 1 cut(s) 288
HaeIII GGCC 3 cut(s) 136, 436, 480
HapII CCGG 1 cut(s) 192
HhaI GCGC 1 cut(s) 367
Hin1II CATG 5 cut(s) 92, 326, 371, 420, 529
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HinfI GANTC 2 cut(s) 373, 455
HpaII CCGG 1 cut(s) 192
HphI GGTGA 1 cut(s) 565
Hpy166II GTNNAC 6 cut(s) 37, 221, 448, 469, 590, 608
Hpy188I TCNGA 3 cut(s) 156, 234, 567
Hpy188III TCNNGA 1 cut(s) 506
Hpy8I GTNNAC 6 cut(s) 37, 221, 448, 469, 590, 608
HpyAV CCTTC 1 cut(s) 285
HpyCH4III ACNGT 2 cut(s) 314, 612
HpyCH4IV ACGT 2 cut(s) 559, 592
HpyCH4V TGCA 4 cut(s) 179, 221, 407, 521
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 1 cut(s) 155
HpySE526I ACGT 2 cut(s) 559, 592
Hsp92II CATG 5 cut(s) 92, 326, 371, 420, 529
HspAI GCGC 1 cut(s) 365
Kzo9I GATC 3 cut(s) 332, 508, 562
LpnPI CCDG 9 cut(s) 25, 39, 105, 132, 205, 417, 444, 473, 519
LweI GCATC 2 cut(s) 394, 508
MaeII ACGT 2 cut(s) 559, 592
MaeIII GTNAC 1 cut(s) 247
MalI GATC 3 cut(s) 334, 510, 564
MboI GATC 3 cut(s) 332, 508, 562
MboII GAAGA 4 cut(s) 103, 106, 141, 476
MflI RGATCY 1 cut(s) 332
MhlI GDGCHC 3 cut(s) 12, 223, 244
MlsI TGGCCA 1 cut(s) 136
MluNI TGGCCA 1 cut(s) 136
MnlI CCTC 4 cut(s) 135, 259, 346, 436
Mox20I TGGCCA 1 cut(s) 136
MscI TGGCCA 1 cut(s) 136
MseI TTAA 1 cut(s) 495
MslI CAYNNNNRTG 2 cut(s) 327, 402
Msp20I TGGCCA 1 cut(s) 136
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 3 cut(s) 120, 192, 432
MvaI CCWGG 2 cut(s) 120, 432
MvnI CGCG 2 cut(s) 46, 365
MwoI GCNNNNNNNGC 1 cut(s) 176
NciI CCSGG 1 cut(s) 192
NcoI CCATGG 2 cut(s) 88, 322
NdeII GATC 3 cut(s) 332, 508, 562
NlaIII CATG 5 cut(s) 92, 326, 371, 420, 529
NlaIV GGNNCC 2 cut(s) 9, 334
NmuCI GTSAC 1 cut(s) 247
NspI RCATGY 1 cut(s) 371
PaeI GCATGC 1 cut(s) 371
PfeI GAWTC 2 cut(s) 373, 455
PflMI CCANNNNNTGG 1 cut(s) 487
PkrI GCNGC 1 cut(s) 289
Ppu21I YACGTR 1 cut(s) 593
Psp6I CCWGG 2 cut(s) 118, 430
PspGI CCWGG 2 cut(s) 118, 430
PspN4I GGNNCC 2 cut(s) 9, 334
PspPI GGNCC 2 cut(s) 122, 434
PstNI CAGNNNCTG 2 cut(s) 290, 464
PsuI RGATCY 1 cut(s) 332
RsaI GTAC 2 cut(s) 468, 595
RsaNI GTAC 2 cut(s) 467, 594
RseI CAYNNNNRTG 2 cut(s) 327, 402
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 3 cut(s) 332, 508, 562
Sau96I GGNCC 2 cut(s) 122, 434
ScrFI CCNGG 3 cut(s) 120, 192, 432
SduI GDGCHC 3 cut(s) 12, 223, 244
SetI ASST 6 cut(s) 127, 212, 388, 447, 562, 595
SfaNI GCATC 2 cut(s) 394, 508
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 2 cut(s) 327, 402
SmlI CTYRAG 1 cut(s) 77
SmoI CTYRAG 1 cut(s) 77
SnaBI TACGTA 1 cut(s) 593
SphI GCATGC 1 cut(s) 371
SsiI CCGC 3 cut(s) 70, 287, 377
StyD4I CCNGG 3 cut(s) 118, 190, 430
StyI CCWWGG 2 cut(s) 88, 322
TaaI ACNGT 2 cut(s) 314, 612
TaiI ACGT 2 cut(s) 562, 595
TaqI TCGA 2 cut(s) 127, 173
TatI WGTACW 1 cut(s) 466
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 2 cut(s) 373, 455
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TseFI GTSAC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 247
Van91I CCANNNNNTGG 1 cut(s) 487
VneI GTGCAC 1 cut(s) 219
VpaK11BI GGWCC 1 cut(s) 122
XceI RCATGY 1 cut(s) 371
XmiI GTMKAC 2 cut(s) 447, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.