MD07G1165400.v1.1

Metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0019772
Physical Location & Seq
Reverse (-)
24026544 .. 24027629
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1165400.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGAAAAATTTTACTTGTGAACAAGGCACAAACTTTGTTGATATGCTGCAATGCTCATCTATAAATAGAGGCAAGGGAACTCAAGACCAACACAATAAAACCCTTACTCATCTCTCTATCTATCTAGCTACCAAATCCCGGAAATGGCCTCCAAAACCTTGCGTAGCCTTATCCCTCCTGATCTTTGTGTTGCTATCTCAAAAAACCCTTGCAAAACCTACTGATGACCATGATCATAAGCATGACTCCTTCGAGTTCATTCAATACTTGGAGGGATGCCACAAGGGTCAAAACGTCAGTGGGTTGCAAGAGCTCAAAAAACACCTCACCAAATTCGGCTACTTTAATTATGATCACTCAAAACACGCGGACAATGACGAGTTTGATGACCTTCTAGAGTCCGCCATCAAGTCATACCAGAAAAGCTACCATTTAAAAGTCACCGGAAGCTTAGACACTACCACAGCGAAACAAATGATGGTGCCAAGATGTGGTGTGCCAGATGTTGTCAACGGCACGCGAAAGGGAAGCAAAAAGTACAACCGCAAACTCAAGTCCCTCCATGGAGTTTCTCATTATGAGTTCTTCCCTGGACCGCTAAGATGGCCATCTACACAAACCCATCTGAGATATCGCTTTGCTTCCAGTGCAAGGCAAGTCCCCGGCACAGAGAATGTAAGGTCTATATGTGCACAAGCATTTCAGAGATGGGCACAAGTGACCACTTTTACATTTGAGGAAGTTCCTTCTACTTCAGCGGCTGACATTACCATTGGCTTCCACCGTGGCAACCATGGAGATAGGTCCCCATTTGACGGCTTCAAAGGGACTTACGCGCATGCGAATCCGCCTGGAGGTGGAAACTTCCATTTTGATGCATATGAGACATGGAGTGCCAATCCAGGGCCGAATGAGATAGACTTGGAATCCGTTTCTGTACACGAAATAGGCCATCTTCTGGGGCTTAATCACAGTCCTGACCTCCCCGATGCAATCATGTATCCCTACTTTGAATATGGGATGGTGAAACGTGATCTGAATAGGGATGATATTGATGGTATACGTGCTTTGTATGGTTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

362

Amino Acids

40.82

Weight (kDa)

8.12

Isoelectric Point (pI)

30.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 101 - 159 5.4e-11 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 202 - 359 1.5e-45 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019772)

Species Orthologous Gene IDs
malus_domestica MD01G1098700.v1.1 MD07G1165400.v1.1
prunus_persica Prupe.2G204200_v2.0.a1 Prupe.2G204300_v2.0.a1 Prupe.2G204500_v2.0.a1
pyrus_communis pycom01g12590 pycom07g16090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 481
AccB7I CCANNNNNTGG 2 cut(s) 491, 958
AccI GTMKAC 1 cut(s) 1060
AccII CGCG 3 cut(s) 368, 520, 836
AciI CCGC 6 cut(s) 368, 402, 544, 596, 758, 848
AcoI YGGCCR 1 cut(s) 605
AcsI RAATTY 2 cut(s) 7, 332
AcuI CTGAAG 1 cut(s) 738
AfaI GTAC 2 cut(s) 539, 939
AfiI CCNNNNNNNGG 9 cut(s) 138, 144, 491, 651, 815, 854, 857, 903, 958
AgsI TTSAA 3 cut(s) 263, 823, 1013
AjnI CCWGG 3 cut(s) 589, 850, 901
AluBI AGCT 4 cut(s) 128, 313, 426, 450
AluI AGCT 4 cut(s) 128, 313, 426, 450
Alw21I GWGCWC 2 cut(s) 315, 694
Alw26I GTCTC 1 cut(s) 878
Alw44I GTGCAC 1 cut(s) 690
AlwNI CAGNNNCTG 1 cut(s) 761
AoxI GGCC 4 cut(s) 146, 605, 905, 949
ApaLI GTGCAC 1 cut(s) 690
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 2 cut(s) 7, 332
AspLEI GCGC 1 cut(s) 838
AspS9I GGNCC 3 cut(s) 593, 804, 905
AsuC2I CCSGG 2 cut(s) 139, 663
AsuHPI GGTGA 3 cut(s) 319, 433, 1036
AvaII GGWCC 2 cut(s) 593, 804
BaeGI GKGCMC 2 cut(s) 694, 715
BalI TGGCCA 1 cut(s) 607
BanI GGYRCC 1 cut(s) 481
BanII GRGCYC 1 cut(s) 315
Bbv12I GWGCWC 2 cut(s) 315, 694
BbvI GCAGC 1 cut(s) 33
BccI CCATC 9 cut(s) 413, 472, 597, 616, 630, 702, 960, 1015, 1049
BceAI ACGGC 2 cut(s) 529, 832
BciT130I CCWGG 3 cut(s) 591, 852, 903
BciVI GTATCC 1 cut(s) 1011
BclI TGATCA 2 cut(s) 232, 352
BcnI CCSGG 2 cut(s) 139, 663
BcoDI GTCTC 1 cut(s) 878
BfaI CTAG 2 cut(s) 125, 395
BfuI GTATCC 1 cut(s) 1011
BisI GCNGC 2 cut(s) 47, 759
BlsI GCNGC 2 cut(s) 48, 760
Bme1390I CCNGG 5 cut(s) 139, 591, 663, 852, 903
Bme18I GGWCC 2 cut(s) 593, 804
BmgT120I GGNCC 3 cut(s) 593, 804, 905
BmiI GGNNCC 2 cut(s) 483, 806
BmrFI CCNGG 5 cut(s) 139, 591, 663, 852, 903
BmsI GCATC 3 cut(s) 266, 865, 979
BpmI CTGGAG 1 cut(s) 873
BpuEI CTTGAG 2 cut(s) 66, 536
BpuMI CCSGG 2 cut(s) 139, 663
BsaAI YACGTR 1 cut(s) 1064
BsaBI GATNNNNATC 1 cut(s) 607
BsaJI CCNNGG 6 cut(s) 562, 589, 661, 784, 793, 902
BsaWI WCCGGW 1 cut(s) 443
Bsc4I CCNNNNNNNGG 9 cut(s) 138, 144, 491, 651, 815, 854, 857, 903, 958
Bse1I ACTGG 1 cut(s) 645
Bse3DI GCAATG 1 cut(s) 56
Bse8I GATNNNNATC 1 cut(s) 607
BseBI CCWGG 3 cut(s) 591, 852, 903
BseDI CCNNGG 6 cut(s) 562, 589, 661, 784, 793, 902
BseGI GGATG 3 cut(s) 281, 1026, 1051
BseJI GATNNNNATC 1 cut(s) 607
BseLI CCNNNNNNNGG 9 cut(s) 138, 144, 491, 651, 815, 854, 857, 903, 958
BseMI GCAATG 1 cut(s) 56
BseMII CTCAG 1 cut(s) 617
BseNI ACTGG 1 cut(s) 645
BseSI GKGCMC 2 cut(s) 694, 715
BseXI GCAGC 1 cut(s) 33
Bsh1236I CGCG 3 cut(s) 368, 520, 836
BshFI GGCC 4 cut(s) 148, 607, 907, 951
BshNI GGYRCC 1 cut(s) 481
BsiHKAI GWGCWC 2 cut(s) 315, 694
BsiSI CCGG 3 cut(s) 139, 444, 663
BslFI GGGAC 4 cut(s) 541, 644, 790, 841
BslI CCNNNNNNNGG 9 cut(s) 138, 144, 491, 651, 815, 854, 857, 903, 958
BsmAI GTCTC 1 cut(s) 878
BsmFI GGGAC 4 cut(s) 541, 644, 790, 841
BsnI GGCC 4 cut(s) 148, 607, 907, 951
Bsp1286I GDGCHC 3 cut(s) 315, 694, 715
Bsp1407I TGTACA 1 cut(s) 937
Bsp143I GATC 4 cut(s) 180, 232, 352, 1033
Bsp19I CCATGG 2 cut(s) 562, 793
BspACI CCGC 6 cut(s) 368, 402, 544, 596, 758, 848
BspANI GGCC 4 cut(s) 148, 607, 907, 951
BspCNI CTCAG 1 cut(s) 618
BspFNI CGCG 3 cut(s) 368, 520, 836
BspLI GGNNCC 2 cut(s) 483, 806
BspT107I GGYRCC 1 cut(s) 481
BsrDI GCAATG 1 cut(s) 56
BsrGI TGTACA 1 cut(s) 937
BsrI ACTGG 1 cut(s) 645
BssECI CCNNGG 6 cut(s) 562, 589, 661, 784, 793, 902
BssMI GATC 4 cut(s) 180, 232, 352, 1033
BssNAI GTATAC 1 cut(s) 1061
BssT1I CCWWGG 2 cut(s) 562, 793
Bst1107I GTATAC 1 cut(s) 1061
Bst2UI CCWGG 3 cut(s) 591, 852, 903
Bst4CI ACNGT 3 cut(s) 785, 974, 1083
BstAUI TGTACA 1 cut(s) 937
BstBAI YACGTR 1 cut(s) 1064
BstC8I GCNNGC 2 cut(s) 518, 840
BstDEI CTNAG 3 cut(s) 451, 599, 626
BstDSI CCRYGG 3 cut(s) 562, 784, 793
BstF5I GGATG 3 cut(s) 281, 1026, 1051
BstFNI CGCG 3 cut(s) 368, 520, 836
BstHHI GCGC 1 cut(s) 838
BstKTI GATC 4 cut(s) 183, 235, 355, 1036
BstMAI GTCTC 1 cut(s) 878
BstMBI GATC 4 cut(s) 180, 232, 352, 1033
BstMWI GCNNNNNNNGC 2 cut(s) 604, 647
BstNI CCWGG 3 cut(s) 591, 852, 903
BstNSI RCATGY 1 cut(s) 842
BstSCI CCNGG 5 cut(s) 137, 589, 661, 850, 901
BstSLI GKGCMC 2 cut(s) 694, 715
BstUI CGCG 3 cut(s) 368, 520, 836
BstV1I GCAGC 1 cut(s) 33
BstZ17I GTATAC 1 cut(s) 1061
BsuI GTATCC 1 cut(s) 1011
BsuRI GGCC 4 cut(s) 148, 607, 907, 951
BtgI CCRYGG 3 cut(s) 562, 784, 793
BtsCI GGATG 3 cut(s) 281, 1026, 1051
BtsIMutI CAGTG 2 cut(s) 304, 652
Cac8I GCNNGC 2 cut(s) 518, 840
CaiI CAGNNNCTG 1 cut(s) 761
CfoI GCGC 1 cut(s) 838
Cfr13I GGNCC 3 cut(s) 593, 804, 905
Csp6I GTAC 2 cut(s) 538, 938
CviAII CATG 7 cut(s) 230, 242, 563, 794, 839, 888, 997
CviQI GTAC 2 cut(s) 538, 938
DdeI CTNAG 3 cut(s) 451, 599, 626
DpnI GATC 4 cut(s) 182, 234, 354, 1035
DpnII GATC 4 cut(s) 180, 232, 352, 1033
DraI TTTAAA 1 cut(s) 435
EaeI YGGCCR 1 cut(s) 605
EciI GGCGGA 2 cut(s) 391, 837
Ecl136II GAGCTC 1 cut(s) 313
Eco130I CCWWGG 2 cut(s) 562, 793
Eco24I GRGCYC 1 cut(s) 315
Eco32I GATATC 1 cut(s) 632
Eco47I GGWCC 2 cut(s) 593, 804
Eco53kI GAGCTC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 738
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 1 cut(s) 804
EcoRII CCWGG 3 cut(s) 589, 850, 901
EcoRV GATATC 1 cut(s) 632
EcoT14I CCWWGG 2 cut(s) 562, 793
EcoT22I ATGCAT 1 cut(s) 880
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 2 cut(s) 562, 793
FaeI CATG 7 cut(s) 233, 245, 566, 797, 842, 891, 1000
FaqI GGGAC 4 cut(s) 541, 644, 790, 841
FatI CATG 7 cut(s) 229, 241, 562, 793, 838, 887, 996
FauNDI CATATG 1 cut(s) 880
FbaI TGATCA 2 cut(s) 232, 352
FblI GTMKAC 1 cut(s) 1060
Fnu4HI GCNGC 2 cut(s) 47, 759
FokI GGATG 3 cut(s) 288, 1033, 1058
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 2 cut(s) 47, 759
FspBI CTAG 2 cut(s) 125, 395
GlaI GCGC 1 cut(s) 837
GluI GCNGC 2 cut(s) 47, 759
GsuI CTGGAG 1 cut(s) 873
HaeIII GGCC 4 cut(s) 148, 607, 907, 951
HapII CCGG 3 cut(s) 139, 444, 663
HhaI GCGC 1 cut(s) 838
Hin1II CATG 7 cut(s) 233, 245, 566, 797, 842, 891, 1000
Hin6I GCGC 1 cut(s) 836
HinP1I GCGC 1 cut(s) 836
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 448
HinfI GANTC 4 cut(s) 245, 398, 844, 926
HpaII CCGG 3 cut(s) 139, 444, 663
HphI GGTGA 3 cut(s) 319, 433, 1036
Hpy166II GTNNAC 6 cut(s) 20, 511, 692, 940, 1061, 1079
Hpy188I TCNGA 3 cut(s) 627, 705, 1038
Hpy188III TCNNGA 4 cut(s) 83, 178, 395, 977
Hpy8I GTNNAC 6 cut(s) 20, 511, 692, 940, 1061, 1079
HpyAV CCTTC 3 cut(s) 259, 401, 756
HpyCH4III ACNGT 3 cut(s) 785, 974, 1083
HpyCH4IV ACGT 3 cut(s) 294, 1030, 1063
HpyCH4V TGCA 7 cut(s) 49, 212, 307, 650, 692, 878, 992
HpyF10VI GCNNNNNNNGC 2 cut(s) 604, 647
HpyF3I CTNAG 3 cut(s) 451, 599, 626
HpySE526I ACGT 3 cut(s) 294, 1030, 1063
Hsp92II CATG 7 cut(s) 233, 245, 566, 797, 842, 891, 1000
HspAI GCGC 1 cut(s) 836
Ksp22I TGATCA 2 cut(s) 232, 352
Kzo9I GATC 4 cut(s) 180, 232, 352, 1033
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 3 cut(s) 266, 865, 979
MaeI CTAG 2 cut(s) 125, 395
MaeII ACGT 3 cut(s) 294, 1030, 1063
MaeIII GTNAC 2 cut(s) 439, 718
MalI GATC 4 cut(s) 182, 234, 354, 1035
MboI GATC 4 cut(s) 180, 232, 352, 1033
MboII GAAGA 2 cut(s) 577, 947
MhlI GDGCHC 3 cut(s) 315, 694, 715
MlsI TGGCCA 1 cut(s) 607
MluCI AATT 3 cut(s) 7, 332, 346
MluNI TGGCCA 1 cut(s) 607
MlyI GAGTC 2 cut(s) 239, 407
MnlI CCTC 9 cut(s) 62, 159, 185, 265, 335, 569, 730, 848, 992
Mox20I TGGCCA 1 cut(s) 607
Mph1103I ATGCAT 1 cut(s) 880
MscI TGGCCA 1 cut(s) 607
MseI TTAA 3 cut(s) 345, 434, 966
MslI CAYNNNNRTG 2 cut(s) 240, 873
Msp20I TGGCCA 1 cut(s) 607
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 3 cut(s) 139, 444, 663
MspR9I CCNGG 5 cut(s) 139, 591, 663, 852, 903
MvaI CCWGG 3 cut(s) 591, 852, 903
MvnI CGCG 3 cut(s) 368, 520, 836
MwoI GCNNNNNNNGC 2 cut(s) 604, 647
NciI CCSGG 2 cut(s) 139, 663
NcoI CCATGG 2 cut(s) 562, 793
NdeI CATATG 1 cut(s) 880
NdeII GATC 4 cut(s) 180, 232, 352, 1033
NlaIII CATG 7 cut(s) 233, 245, 566, 797, 842, 891, 1000
NlaIV GGNNCC 2 cut(s) 483, 806
NmuCI GTSAC 2 cut(s) 439, 718
NsiI ATGCAT 1 cut(s) 880
NspI RCATGY 1 cut(s) 842
PaeI GCATGC 1 cut(s) 842
PfeI GAWTC 2 cut(s) 844, 926
PflMI CCANNNNNTGG 2 cut(s) 491, 958
PfoI TCCNGGA 1 cut(s) 137
PkrI GCNGC 2 cut(s) 48, 760
PleI GAGTC 2 cut(s) 239, 406
PpsI GAGTC 2 cut(s) 239, 406
Ppu21I YACGTR 1 cut(s) 1064
PpuMI RGGWCCY 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 804
Psp6I CCWGG 3 cut(s) 589, 850, 901
PspGI CCWGG 3 cut(s) 589, 850, 901
PspN4I GGNNCC 2 cut(s) 483, 806
PspPI GGNCC 3 cut(s) 593, 804, 905
PspPPI RGGWCCY 1 cut(s) 804
PstNI CAGNNNCTG 1 cut(s) 761
RsaI GTAC 2 cut(s) 539, 939
RsaNI GTAC 2 cut(s) 538, 938
RseI CAYNNNNRTG 2 cut(s) 240, 873
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 3 cut(s) 345, 434, 966
SatI GCNGC 2 cut(s) 47, 759
Sau3AI GATC 4 cut(s) 180, 232, 352, 1033
Sau96I GGNCC 3 cut(s) 593, 804, 905
SchI GAGTC 2 cut(s) 239, 407
ScrFI CCNGG 5 cut(s) 139, 591, 663, 852, 903
SduI GDGCHC 3 cut(s) 315, 694, 715
SfaNI GCATC 3 cut(s) 266, 865, 979
SinI GGWCC 2 cut(s) 593, 804
SmiMI CAYNNNNRTG 2 cut(s) 240, 873
SmlI CTYRAG 2 cut(s) 81, 551
SmoI CTYRAG 2 cut(s) 81, 551
SphI GCATGC 1 cut(s) 842
Sse9I AATT 3 cut(s) 7, 332, 346
SsiI CCGC 6 cut(s) 368, 402, 544, 596, 758, 848
SspMI CTAG 2 cut(s) 125, 395
SstI GAGCTC 1 cut(s) 315
StyD4I CCNGG 5 cut(s) 137, 589, 661, 850, 901
StyI CCWWGG 2 cut(s) 562, 793
TaaI ACNGT 3 cut(s) 785, 974, 1083
TaiI ACGT 3 cut(s) 297, 1033, 1066
TaqI TCGA 1 cut(s) 252
TasI AATT 3 cut(s) 7, 332, 346
TatI WGTACW 2 cut(s) 537, 937
TauI GCSGC 1 cut(s) 761
TfiI GAWTC 2 cut(s) 844, 926
Tru1I TTAA 3 cut(s) 345, 434, 966
Tru9I TTAA 3 cut(s) 345, 434, 966
TscAI CASTG 2 cut(s) 304, 652
TseFI GTSAC 2 cut(s) 439, 718
TseI GCWGC 1 cut(s) 46
Tsp45I GTSAC 2 cut(s) 439, 718
TspDTI ATGAA 2 cut(s) 17, 247
TspGWI ACGGA 1 cut(s) 919
TspRI CASTG 2 cut(s) 304, 652
Van91I CCANNNNNTGG 2 cut(s) 491, 958
VneI GTGCAC 1 cut(s) 690
VpaK11BI GGWCC 2 cut(s) 593, 804
XapI RAATTY 2 cut(s) 7, 332
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 1060
XspI CTAG 2 cut(s) 125, 395
Zsp2I ATGCAT 1 cut(s) 880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.