pycom01g12590

Metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019772
Physical Location & Seq
Reverse (-)
13295066 .. 13296031
966 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g12590.1

Sequence Viewer

Length: 966 bp
ATGCCGGCAATGGCCTCGAAAACCGCCTCGGTTTGCGTAGTCTTTTCCCTTTTGATTTTTGTGTTGCTATCTCAGCAAACTCTTGCAAAACCTACTGATCGATCCCATGGTCATCAGCATGACTCCTTCAAGTTCATCCAACACTTGGAGGGATGCCACAAGGGTCAAAACGTCAGTGGGTTGCAAGAGCTCAAAAAATACCTCACCAAATTCGGCTACTTGAATTATGATCACTCAAAACACGCCAACGACGATGAGTTTGATGACCTTCTAGAGTCCGCCATCAAGTCATACCAAAAAAGCTTCCACTTGAATGTCACCGGAAGTCTAGACACTACCACGGTGAAACAAATGATGGTGCCAAGATGTGGTGTACCGGATGTTGTCAATGGCACACGAAAGGGAACAAAAAAGCACAACCGCAAGCTCAAGTCCATCCATGGAGTTTCTCATTATGAGTTCTTCTTCCCTGGTCCGCTAAGATGGCCACCTACGAAAACCCATTTGAGATATCGCTTTGCTTCGAGTGCAAGCCAAGTCCCCGGCACAGAGAATGTGACGTCTATATGTGCACAAGCATTTCAGAGATGGGCACAAGTGACCAGTTTCACATTTGAGGAAGTGCCTGCTACTTCATCAGCTGACATTACCATTGGCTTTCACCGGGGCGCCCATGGAGATATGGCCCCATTTGACGGCTTCAAAGGGGCTTTAGCACACGCGAATCCGCCTACAGGTGGTAATTTCCATTTTGATGCAGATGAGAAGTGGAGTGCCAATCCAGGACCCAATGAGGTAGACTTGGAATCCGTCGCTGTGCACGAAATTGGTCATCTTCTAGGACTTGATCACAATCTTGACCTCCCCGATGCGATCATGTATCCCAAATTTTCTTATGGGATTGTAAAACGTGATCTGACTGGGGATGACATTGATGGAATACGTGCTTTGTATGGTTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

322

Amino Acids

35.6

Weight (kDa)

6.83

Isoelectric Point (pI)

29.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 60 - 118 7.9e-13 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 162 - 319 1.1e-45 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019772)

Species Orthologous Gene IDs
malus_domestica MD01G1098700.v1.1 MD07G1165400.v1.1
prunus_persica Prupe.2G204200_v2.0.a1 Prupe.2G204300_v2.0.a1 Prupe.2G204500_v2.0.a1
pyrus_communis pycom01g12590 pycom07g16090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 563
AccB1I GGYRCC 2 cut(s) 358, 668
AccB7I CCANNNNNTGG 2 cut(s) 145, 368
AccI GTMKAC 1 cut(s) 798
AccII CGCG 1 cut(s) 722
AciI CCGC 5 cut(s) 24, 279, 421, 476, 728
AclWI GGATC 1 cut(s) 96
AcoI YGGCCR 1 cut(s) 485
AcsI RAATTY 2 cut(s) 209, 887
AcyI GRCGYC 2 cut(s) 560, 669
AfaI GTAC 1 cut(s) 375
AfiI CCNNNNNNNGG 5 cut(s) 145, 368, 695, 734, 737
AgsI TTSAA 4 cut(s) 130, 223, 313, 703
AjnI CCWGG 2 cut(s) 469, 781
AluBI AGCT 4 cut(s) 190, 303, 427, 641
AluI AGCT 4 cut(s) 190, 303, 427, 641
Alw21I GWGCWC 3 cut(s) 192, 574, 822
Alw44I GTGCAC 2 cut(s) 570, 818
AlwI GGATC 1 cut(s) 96
AoxI GGCC 3 cut(s) 12, 485, 684
ApaLI GTGCAC 2 cut(s) 570, 818
ApoI RAATTY 2 cut(s) 209, 887
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspLEI GCGC 1 cut(s) 671
AspS9I GGNCC 3 cut(s) 473, 685, 785
AsuC2I CCSGG 2 cut(s) 543, 665
AsuHPI GGTGA 4 cut(s) 196, 310, 355, 653
AvaII GGWCC 2 cut(s) 473, 785
BaeGI GKGCMC 3 cut(s) 574, 595, 822
BalI TGGCCA 1 cut(s) 487
BanI GGYRCC 2 cut(s) 358, 668
BanII GRGCYC 1 cut(s) 192
Bbv12I GWGCWC 3 cut(s) 192, 574, 822
BccI CCATC 6 cut(s) 290, 349, 443, 477, 582, 929
BceAI ACGGC 1 cut(s) 712
BcgI CGANNNNNNTGC 1 cut(s) 31
BciT130I CCWGG 2 cut(s) 471, 783
BciVI GTATCC 1 cut(s) 891
BclI TGATCA 2 cut(s) 229, 847
BcnI CCSGG 2 cut(s) 543, 665
BfaI CTAG 3 cut(s) 272, 329, 839
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 1 cut(s) 672
BfuI GTATCC 1 cut(s) 891
Bme1390I CCNGG 4 cut(s) 471, 543, 665, 783
Bme18I GGWCC 2 cut(s) 473, 785
BmgT120I GGNCC 3 cut(s) 473, 685, 785
BmiI GGNNCC 4 cut(s) 360, 670, 687, 787
BmrFI CCNGG 4 cut(s) 471, 543, 665, 783
BmrI ACTGGG 1 cut(s) 930
BmsI GCATC 3 cut(s) 143, 745, 859
BmuI ACTGGG 1 cut(s) 930
BpuEI CTTGAG 1 cut(s) 413
BpuMI CCSGG 2 cut(s) 543, 665
Bsa29I ATCGAT 1 cut(s) 100
BsaAI YACGTR 1 cut(s) 944
BsaBI GATNNNNATC 1 cut(s) 852
BsaHI GRCGYC 2 cut(s) 560, 669
BsaJI CCNNGG 8 cut(s) 27, 106, 339, 439, 469, 541, 664, 673
BsaWI WCCGGW 2 cut(s) 320, 376
Bsc4I CCNNNNNNNGG 5 cut(s) 145, 368, 695, 734, 737
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 2 cut(s) 603, 925
Bse3DI GCAATG 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 852
BseBI CCWGG 2 cut(s) 471, 783
BseCI ATCGAT 1 cut(s) 100
BseDI CCNNGG 8 cut(s) 27, 106, 339, 439, 469, 541, 664, 673
BseGI GGATG 5 cut(s) 135, 158, 385, 435, 931
BseJI GATNNNNATC 1 cut(s) 852
BseLI CCNNNNNNNGG 5 cut(s) 145, 368, 695, 734, 737
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 1 cut(s) 86
BseNI ACTGG 2 cut(s) 603, 925
BseSI GKGCMC 3 cut(s) 574, 595, 822
Bsh1236I CGCG 1 cut(s) 722
BshFI GGCC 3 cut(s) 14, 487, 686
BshNI GGYRCC 2 cut(s) 358, 668
BshVI ATCGAT 1 cut(s) 100
BsiHKAI GWGCWC 3 cut(s) 192, 574, 822
BsiSI CCGG 5 cut(s) 5, 321, 377, 543, 664
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 5 cut(s) 145, 368, 695, 734, 737
BsmFI GGGAC 1 cut(s) 524
BsnI GGCC 3 cut(s) 14, 487, 686
Bsp1286I GDGCHC 4 cut(s) 192, 574, 595, 822
Bsp143I GATC 6 cut(s) 97, 101, 229, 847, 873, 913
Bsp19I CCATGG 3 cut(s) 106, 439, 673
BspACI CCGC 5 cut(s) 24, 279, 421, 476, 728
BspANI GGCC 3 cut(s) 14, 487, 686
BspCNI CTCAG 1 cut(s) 85
BspDI ATCGAT 1 cut(s) 100
BspFNI CGCG 1 cut(s) 722
BspLI GGNNCC 4 cut(s) 360, 670, 687, 787
BspPI GGATC 1 cut(s) 96
BspT107I GGYRCC 2 cut(s) 358, 668
BsrDI GCAATG 1 cut(s) 15
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 2 cut(s) 603, 925
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 8 cut(s) 27, 106, 339, 439, 469, 541, 664, 673
BssMI GATC 6 cut(s) 97, 101, 229, 847, 873, 913
BssNI GRCGYC 2 cut(s) 560, 669
BssT1I CCWWGG 3 cut(s) 106, 439, 673
Bst2UI CCWGG 2 cut(s) 471, 783
Bst4CI ACNGT 2 cut(s) 343, 963
BstACI GRCGYC 2 cut(s) 560, 669
BstBAI YACGTR 1 cut(s) 944
BstC8I GCNNGC 4 cut(s) 6, 425, 532, 627
BstDEI CTNAG 2 cut(s) 72, 479
BstDSI CCRYGG 4 cut(s) 106, 339, 439, 673
BstF5I GGATG 5 cut(s) 135, 158, 385, 435, 931
BstFNI CGCG 1 cut(s) 722
BstH2I RGCGCY 1 cut(s) 672
BstHHI GCGC 1 cut(s) 671
BstKTI GATC 6 cut(s) 100, 104, 232, 850, 876, 916
BstMBI GATC 6 cut(s) 97, 101, 229, 847, 873, 913
BstMWI GCNNNNNNNGC 3 cut(s) 73, 484, 527
BstNI CCWGG 2 cut(s) 471, 783
BstSCI CCNGG 4 cut(s) 469, 541, 663, 781
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 3 cut(s) 574, 595, 822
BstUI CGCG 1 cut(s) 722
Bsu15I ATCGAT 1 cut(s) 100
BsuI GTATCC 1 cut(s) 891
BsuRI GGCC 3 cut(s) 14, 487, 686
BsuTUI ATCGAT 1 cut(s) 100
BtgI CCRYGG 4 cut(s) 106, 339, 439, 673
BtsCI GGATG 5 cut(s) 135, 158, 385, 435, 931
BtsIMutI CAGTG 1 cut(s) 181
Cac8I GCNNGC 4 cut(s) 6, 425, 532, 627
CfoI GCGC 1 cut(s) 671
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 3 cut(s) 473, 685, 785
ClaI ATCGAT 1 cut(s) 100
Csp6I GTAC 1 cut(s) 374
CviAII CATG 5 cut(s) 107, 119, 440, 674, 877
CviQI GTAC 1 cut(s) 374
DdeI CTNAG 2 cut(s) 72, 479
DinI GGCGCC 1 cut(s) 670
DpnI GATC 6 cut(s) 99, 103, 231, 849, 875, 915
DpnII GATC 6 cut(s) 97, 101, 229, 847, 873, 913
EaeI YGGCCR 1 cut(s) 485
EciI GGCGGA 2 cut(s) 268, 717
Ecl136II GAGCTC 1 cut(s) 190
Eco130I CCWWGG 3 cut(s) 106, 439, 673
Eco24I GRGCYC 1 cut(s) 192
Eco32I GATATC 1 cut(s) 512
Eco47I GGWCC 2 cut(s) 473, 785
Eco53kI GAGCTC 1 cut(s) 190
EcoICRI GAGCTC 1 cut(s) 190
EcoO109I RGGNCCY 1 cut(s) 785
EcoRII CCWGG 2 cut(s) 469, 781
EcoRV GATATC 1 cut(s) 512
EcoT14I CCWWGG 3 cut(s) 106, 439, 673
EcoT38I GRGCYC 1 cut(s) 192
EgeI GGCGCC 1 cut(s) 670
EheI GGCGCC 1 cut(s) 670
ErhI CCWWGG 3 cut(s) 106, 439, 673
FaeI CATG 5 cut(s) 110, 122, 443, 677, 880
FaqI GGGAC 1 cut(s) 524
FatI CATG 5 cut(s) 106, 118, 439, 673, 876
FbaI TGATCA 2 cut(s) 229, 847
FblI GTMKAC 1 cut(s) 798
FokI GGATG 5 cut(s) 122, 165, 392, 422, 938
FriOI GRGCYC 1 cut(s) 192
FspBI CTAG 3 cut(s) 272, 329, 839
GlaI GCGC 1 cut(s) 670
HaeII RGCGCY 1 cut(s) 672
HaeIII GGCC 3 cut(s) 14, 487, 686
HapII CCGG 5 cut(s) 5, 321, 377, 543, 664
HhaI GCGC 1 cut(s) 671
Hin1I GRCGYC 2 cut(s) 560, 669
Hin1II CATG 5 cut(s) 110, 122, 443, 677, 880
Hin6I GCGC 1 cut(s) 669
HinP1I GCGC 1 cut(s) 669
HindIII AAGCTT 1 cut(s) 301
HinfI GANTC 4 cut(s) 122, 275, 724, 806
HpaII CCGG 5 cut(s) 5, 321, 377, 543, 664
HphI GGTGA 4 cut(s) 196, 310, 355, 653
Hpy166II GTNNAC 5 cut(s) 374, 572, 799, 820, 959
Hpy188I TCNGA 2 cut(s) 585, 918
Hpy188III TCNNGA 3 cut(s) 272, 329, 857
Hpy8I GTNNAC 5 cut(s) 374, 572, 799, 820, 959
Hpy99I CGWCG 2 cut(s) 254, 815
HpyAV CCTTC 2 cut(s) 136, 278
HpyCH4III ACNGT 2 cut(s) 343, 963
HpyCH4IV ACGT 4 cut(s) 171, 560, 910, 943
HpyCH4V TGCA 6 cut(s) 86, 184, 530, 572, 758, 820
HpyF10VI GCNNNNNNNGC 3 cut(s) 73, 484, 527
HpyF3I CTNAG 2 cut(s) 72, 479
HpySE526I ACGT 4 cut(s) 171, 560, 910, 943
Hsp92I GRCGYC 2 cut(s) 560, 669
Hsp92II CATG 5 cut(s) 110, 122, 443, 677, 880
HspAI GCGC 1 cut(s) 669
KasI GGCGCC 1 cut(s) 668
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 2 cut(s) 229, 847
Kzo9I GATC 6 cut(s) 97, 101, 229, 847, 873, 913
LweI GCATC 3 cut(s) 143, 745, 859
MaeI CTAG 3 cut(s) 272, 329, 839
MaeII ACGT 4 cut(s) 171, 560, 910, 943
MaeIII GTNAC 3 cut(s) 316, 556, 598
MalI GATC 6 cut(s) 99, 103, 231, 849, 875, 915
MboI GATC 6 cut(s) 97, 101, 229, 847, 873, 913
MboII GAAGA 3 cut(s) 454, 457, 827
MhlI GDGCHC 4 cut(s) 192, 574, 595, 822
MlsI TGGCCA 1 cut(s) 487
MluCI AATT 5 cut(s) 209, 223, 742, 825, 887
MluNI TGGCCA 1 cut(s) 487
Mly113I GGCGCC 1 cut(s) 669
MlyI GAGTC 2 cut(s) 116, 284
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 7 cut(s) 25, 37, 142, 212, 610, 787, 872
Mox20I TGGCCA 1 cut(s) 487
MroNI GCCGGC 1 cut(s) 4
MscI TGGCCA 1 cut(s) 487
MslI CAYNNNNRTG 3 cut(s) 117, 312, 753
Msp20I TGGCCA 1 cut(s) 487
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 5 cut(s) 5, 321, 377, 543, 664
MspR9I CCNGG 4 cut(s) 471, 543, 665, 783
MvaI CCWGG 2 cut(s) 471, 783
MvnI CGCG 1 cut(s) 722
MwoI GCNNNNNNNGC 3 cut(s) 73, 484, 527
NaeI GCCGGC 1 cut(s) 6
NarI GGCGCC 1 cut(s) 669
NciI CCSGG 2 cut(s) 543, 665
NcoI CCATGG 3 cut(s) 106, 439, 673
NdeII GATC 6 cut(s) 97, 101, 229, 847, 873, 913
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 5 cut(s) 110, 122, 443, 677, 880
NlaIV GGNNCC 4 cut(s) 360, 670, 687, 787
NmuCI GTSAC 3 cut(s) 316, 556, 598
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 819
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 2 cut(s) 724, 806
PflMI CCANNNNNTGG 2 cut(s) 145, 368
PfoI TCCNGGA 1 cut(s) 781
PleI GAGTC 2 cut(s) 116, 283
PluTI GGCGCC 1 cut(s) 672
PpsI GAGTC 2 cut(s) 116, 283
Ppu21I YACGTR 1 cut(s) 944
PpuMI RGGWCCY 1 cut(s) 785
Psp124BI GAGCTC 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 785
Psp6I CCWGG 2 cut(s) 469, 781
PspGI CCWGG 2 cut(s) 469, 781
PspN4I GGNNCC 4 cut(s) 360, 670, 687, 787
PspPI GGNCC 3 cut(s) 473, 685, 785
PspPPI RGGWCCY 1 cut(s) 785
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 1 cut(s) 375
RsaNI GTAC 1 cut(s) 374
RseI CAYNNNNRTG 3 cut(s) 117, 312, 753
SacI GAGCTC 1 cut(s) 192
Sau3AI GATC 6 cut(s) 97, 101, 229, 847, 873, 913
Sau96I GGNCC 3 cut(s) 473, 685, 785
SchI GAGTC 2 cut(s) 116, 284
ScrFI CCNGG 4 cut(s) 471, 543, 665, 783
SduI GDGCHC 4 cut(s) 192, 574, 595, 822
SfaNI GCATC 3 cut(s) 143, 745, 859
SfcI CTRYAG 1 cut(s) 732
SfoI GGCGCC 1 cut(s) 670
SinI GGWCC 2 cut(s) 473, 785
SmiMI CAYNNNNRTG 3 cut(s) 117, 312, 753
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 5 cut(s) 209, 223, 742, 825, 887
SsiI CCGC 5 cut(s) 24, 279, 421, 476, 728
SspDI GGCGCC 1 cut(s) 668
SspMI CTAG 3 cut(s) 272, 329, 839
SstI GAGCTC 1 cut(s) 192
StyD4I CCNGG 4 cut(s) 469, 541, 663, 781
StyI CCWWGG 3 cut(s) 106, 439, 673
TaaI ACNGT 2 cut(s) 343, 963
TaiI ACGT 4 cut(s) 174, 563, 913, 946
TaqI TCGA 3 cut(s) 17, 100, 524
TasI AATT 5 cut(s) 209, 223, 742, 825, 887
TfiI GAWTC 2 cut(s) 724, 806
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 3 cut(s) 316, 556, 598
Tsp45I GTSAC 3 cut(s) 316, 556, 598
TspDTI ATGAA 2 cut(s) 124, 624
TspGWI ACGGA 1 cut(s) 799
TspRI CASTG 1 cut(s) 181
Van91I CCANNNNNTGG 2 cut(s) 145, 368
VneI GTGCAC 2 cut(s) 570, 818
VpaK11BI GGWCC 2 cut(s) 473, 785
XapI RAATTY 2 cut(s) 209, 887
XbaI TCTAGA 2 cut(s) 271, 328
XmiI GTMKAC 1 cut(s) 798
XspI CTAG 3 cut(s) 272, 329, 839
ZraI GACGTC 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.