Prupe.2G204300_v2.0.a1

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0019772
Physical Location & Seq
Reverse (-)
23961390 .. 23962319
930 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G204300.1

Sequence Viewer

Length: 930 bp
ATGCCTGCAATGGCCTCAAAAACCATCTCAGTTTGTGTAGCCTTTTTCCTCATCTTTGTGTTGCTCTCTCAAAACACTCATTCCAAATCTACTCATGACCAGCATGACCCCTTCAAGTTCATCCAACACTTGGAGGGATGCCACAAGGGTCAAAATGTGAGCGGGCTGCAAGAGCTCAAAAAATACCTCACCAAATTTGGCTACCTGAATTATGATCATTCAAAGCATGCCAACGACGAGGAGTTTGATGACATTCTAGAGTCTGCCATCAAGTCATACCAGAAAAGCTATCACTTAAAAGTCACTGGAACTCTAGACACCACCACGGGGAAACAGATGATGATGCCAAGATGTGGTGTGCCGGATGTTGTCAATGGCACTCGAAAGGGCAGCAAGAAACACAACCATAAGCACAAGTCTATCCATGGGGTTGCTCATTATGAGTTCTTCCCCGGACCGCGAAGATGGTCCAAAACCCATCTGACGTATCGCTTTAGTTCAAGTGTGAACCAAGTCCCTGGCACACAGAATGTAAGGTCTATATGTGCACAAGCATTTCAGAGATGGGCACAAGTGACCAGTTTTACATTTGAGGAAGTCCCTGCTACTTCAGCAGCTGATATTACCATTGGATTTCACAGGGGCAACCATGGAGATGGTTCCGCATTCGATGGCCCCAGAGGGACTTTCGCGCACGCTAATCCGCCTAGAGGTGGGAATTTCCATTATGATGCAGATGAGAGATGGAGTAGCAATCCAGGACCAAATGAGGTAGACTTGGAATCTGTCGCCGTGCACGAAATAGGCCATCTGCTGGGGCTTAATCACAATCCTGACCTCCCGGACGCAATCATGTATCCCTACTTCAATTATGGGAGTATAAAACGGAATCTGCATAGGGATGACAATGGGATGTTAACACTACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

310

Amino Acids

34.72

Weight (kDa)

8.27

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019772)

Species Orthologous Gene IDs
malus_domestica MD01G1098700.v1.1 MD07G1165400.v1.1
prunus_persica Prupe.2G204200_v2.0.a1 Prupe.2G204300_v2.0.a1 Prupe.2G204500_v2.0.a1
pyrus_communis pycom01g12590 pycom07g16090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 130, 353, 814
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 774
AccII CGCG 2 cut(s) 460, 692
AciI CCGC 4 cut(s) 162, 458, 663, 704
AcsI RAATTY 2 cut(s) 194, 718
AcuI CTGAAG 1 cut(s) 594
AfiI CCNNNNNNNGG 6 cut(s) 130, 327, 353, 710, 713, 814
AgsI TTSAA 4 cut(s) 115, 222, 501, 868
AjnI CCWGG 2 cut(s) 517, 757
AluBI AGCT 3 cut(s) 175, 288, 617
AluI AGCT 3 cut(s) 175, 288, 617
Alw21I GWGCWC 3 cut(s) 177, 550, 798
Alw44I GTGCAC 2 cut(s) 546, 794
AlwNI CAGNNNCTG 1 cut(s) 617
AoxI GGCC 3 cut(s) 12, 673, 805
ApaLI GTGCAC 2 cut(s) 546, 794
ApeKI GCWGC 3 cut(s) 166, 390, 614
ApoI RAATTY 2 cut(s) 194, 718
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 1 cut(s) 694
AspS9I GGNCC 4 cut(s) 455, 468, 674, 761
AsuC2I CCSGG 2 cut(s) 453, 842
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 3 cut(s) 455, 468, 761
BaeGI GKGCMC 3 cut(s) 550, 571, 798
BanII GRGCYC 1 cut(s) 177
BarI GAAGNNNNNNTAC 2 cut(s) 848, 880
Bbv12I GWGCWC 3 cut(s) 177, 550, 798
BbvI GCAGC 3 cut(s) 153, 402, 626
BccI CCATC 9 cut(s) 32, 275, 459, 486, 558, 650, 665, 738, 816
BceAI ACGGC 1 cut(s) 776
BciT130I CCWGG 2 cut(s) 519, 759
BciVI GTATCC 1 cut(s) 867
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 453, 842
BfaI CTAG 3 cut(s) 257, 314, 708
BfuI GTATCC 1 cut(s) 867
BisI GCNGC 3 cut(s) 167, 391, 615
BlsI GCNGC 3 cut(s) 168, 392, 616
Bme1390I CCNGG 4 cut(s) 453, 519, 759, 842
Bme18I GGWCC 3 cut(s) 455, 468, 761
BmgT120I GGNCC 4 cut(s) 455, 468, 674, 761
BmiI GGNNCC 2 cut(s) 661, 676
BmrFI CCNGG 4 cut(s) 453, 519, 759, 842
BmsI GCATC 3 cut(s) 128, 333, 721
BpuMI CCSGG 2 cut(s) 453, 842
BsaJI CCNNGG 5 cut(s) 324, 424, 451, 517, 649
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 327, 353, 710, 713, 814
Bse1I ACTGG 2 cut(s) 310, 579
Bse3DI GCAATG 1 cut(s) 15
BseBI CCWGG 2 cut(s) 519, 759
BseDI CCNNGG 5 cut(s) 324, 424, 451, 517, 649
BseGI GGATG 5 cut(s) 120, 143, 370, 907, 918
BseLI CCNNNNNNNGG 6 cut(s) 130, 327, 353, 710, 713, 814
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 2 cut(s) 310, 579
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 3 cut(s) 550, 571, 798
BseXI GCAGC 3 cut(s) 153, 402, 626
BseYI CCCAGC 1 cut(s) 814
Bsh1236I CGCG 2 cut(s) 460, 692
BshFI GGCC 3 cut(s) 14, 675, 807
BsiHKAI GWGCWC 3 cut(s) 177, 550, 798
BsiSI CCGG 3 cut(s) 362, 453, 842
BslFI GGGAC 3 cut(s) 500, 584, 697
BslI CCNNNNNNNGG 6 cut(s) 130, 327, 353, 710, 713, 814
BsmFI GGGAC 3 cut(s) 500, 584, 697
BsmI GAATGC 1 cut(s) 665
BsnI GGCC 3 cut(s) 14, 675, 807
Bsp1286I GDGCHC 4 cut(s) 177, 550, 571, 798
Bsp143I GATC 1 cut(s) 214
Bsp19I CCATGG 2 cut(s) 424, 649
BspACI CCGC 4 cut(s) 162, 458, 663, 704
BspANI GGCC 3 cut(s) 14, 675, 807
BspCNI CTCAG 1 cut(s) 41
BspFNI CGCG 2 cut(s) 460, 692
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 661, 676
BsrBI CCGCTC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 15
BsrI ACTGG 2 cut(s) 310, 579
BssECI CCNNGG 5 cut(s) 324, 424, 451, 517, 649
BssMI GATC 1 cut(s) 214
BssT1I CCWWGG 2 cut(s) 424, 649
Bst2UI CCWGG 2 cut(s) 519, 759
BstC8I GCNNGC 4 cut(s) 6, 164, 228, 696
BstDEI CTNAG 1 cut(s) 28
BstDSI CCRYGG 3 cut(s) 324, 424, 649
BstF5I GGATG 5 cut(s) 120, 143, 370, 907, 918
BstFNI CGCG 2 cut(s) 460, 692
BstHHI GCGC 1 cut(s) 694
BstKTI GATC 1 cut(s) 217
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 2 cut(s) 172, 611
BstNI CCWGG 2 cut(s) 519, 759
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 4 cut(s) 451, 517, 757, 840
BstSLI GKGCMC 3 cut(s) 550, 571, 798
BstUI CGCG 2 cut(s) 460, 692
BstV1I GCAGC 3 cut(s) 153, 402, 626
BstXI CCANNNNNNTGG 2 cut(s) 518, 656
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 3 cut(s) 14, 675, 807
BtgI CCRYGG 3 cut(s) 324, 424, 649
BtsCI GGATG 5 cut(s) 120, 143, 370, 907, 918
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 4 cut(s) 6, 164, 228, 696
CaiI CAGNNNCTG 1 cut(s) 617
CciI TCATGA 1 cut(s) 94
CfoI GCGC 1 cut(s) 694
Cfr13I GGNCC 4 cut(s) 455, 468, 674, 761
CpoI CGGWCCG 1 cut(s) 455
CseI GACGC 1 cut(s) 854
CspI CGGWCCG 1 cut(s) 455
CviAII CATG 6 cut(s) 95, 104, 227, 425, 650, 853
DdeI CTNAG 1 cut(s) 28
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EciI GGCGGA 1 cut(s) 693
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 424, 649
Eco24I GRGCYC 1 cut(s) 177
Eco47I GGWCC 3 cut(s) 455, 468, 761
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 594
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 517, 757
EcoT14I CCWWGG 2 cut(s) 424, 649
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 2 cut(s) 424, 649
FaeI CATG 6 cut(s) 98, 107, 230, 428, 653, 856
FaqI GGGAC 3 cut(s) 500, 584, 697
FatI CATG 6 cut(s) 94, 103, 226, 424, 649, 852
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 774
Fnu4HI GCNGC 3 cut(s) 167, 391, 615
FokI GGATG 5 cut(s) 107, 150, 377, 914, 925
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 3 cut(s) 167, 391, 615
FspBI CTAG 3 cut(s) 257, 314, 708
GlaI GCGC 1 cut(s) 693
GluI GCNGC 3 cut(s) 167, 391, 615
GsaI CCCAGC 1 cut(s) 818
HaeIII GGCC 3 cut(s) 14, 675, 807
HapII CCGG 3 cut(s) 362, 453, 842
HgaI GACGC 1 cut(s) 854
HhaI GCGC 1 cut(s) 694
Hin1II CATG 6 cut(s) 98, 107, 230, 428, 653, 856
Hin6I GCGC 1 cut(s) 692
HinP1I GCGC 1 cut(s) 692
HincII GTYRAC 1 cut(s) 918
HindII GTYRAC 1 cut(s) 918
HinfI GANTC 3 cut(s) 260, 782, 889
HpaI GTTAAC 1 cut(s) 918
HpaII CCGG 3 cut(s) 362, 453, 842
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 5 cut(s) 508, 548, 775, 796, 918
Hpy188I TCNGA 2 cut(s) 483, 561
Hpy188III TCNNGA 4 cut(s) 95, 257, 314, 833
Hpy8I GTNNAC 5 cut(s) 508, 548, 775, 796, 918
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 121
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 6 cut(s) 8, 169, 548, 734, 796, 895
HpyF10VI GCNNNNNNNGC 2 cut(s) 172, 611
HpyF3I CTNAG 1 cut(s) 28
HpySE526I ACGT 1 cut(s) 485
Hsp92II CATG 6 cut(s) 98, 107, 230, 428, 653, 856
HspAI GCGC 1 cut(s) 692
Ksp22I TGATCA 1 cut(s) 214
KspAI GTTAAC 1 cut(s) 918
Kzo9I GATC 1 cut(s) 214
Lsp1109I GCAGC 3 cut(s) 153, 402, 626
LweI GCATC 3 cut(s) 128, 333, 721
MaeI CTAG 3 cut(s) 257, 314, 708
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 2 cut(s) 301, 574
MalI GATC 1 cut(s) 216
MbiI CCGCTC 1 cut(s) 162
MboI GATC 1 cut(s) 214
MboII GAAGA 2 cut(s) 439, 474
MhlI GDGCHC 4 cut(s) 177, 550, 571, 798
MluCI AATT 4 cut(s) 194, 208, 718, 868
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 148
MseI TTAA 3 cut(s) 296, 822, 917
MslI CAYNNNNRTG 4 cut(s) 56, 654, 729, 900
MspA1I CMGCKG 1 cut(s) 617
MspI CCGG 3 cut(s) 362, 453, 842
MspR9I CCNGG 4 cut(s) 453, 519, 759, 842
Mva1269I GAATGC 1 cut(s) 665
MvaI CCWGG 2 cut(s) 519, 759
MvnI CGCG 2 cut(s) 460, 692
MwoI GCNNNNNNNGC 2 cut(s) 172, 611
NciI CCSGG 2 cut(s) 453, 842
NcoI CCATGG 2 cut(s) 424, 649
NdeII GATC 1 cut(s) 214
NlaIII CATG 6 cut(s) 98, 107, 230, 428, 653, 856
NlaIV GGNNCC 2 cut(s) 661, 676
NmuCI GTSAC 2 cut(s) 301, 574
NspI RCATGY 1 cut(s) 230
PaeI GCATGC 1 cut(s) 230
PagI TCATGA 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 795
PctI GAATGC 1 cut(s) 665
PfeI GAWTC 2 cut(s) 782, 889
PflMI CCANNNNNTGG 3 cut(s) 130, 353, 814
PfoI TCCNGGA 2 cut(s) 757, 840
PkrI GCNGC 3 cut(s) 168, 392, 616
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 517, 757
PspFI CCCAGC 1 cut(s) 814
PspGI CCWGG 2 cut(s) 517, 757
PspN4I GGNNCC 2 cut(s) 661, 676
PspPI GGNCC 4 cut(s) 455, 468, 674, 761
PstNI CAGNNNCTG 1 cut(s) 617
PvuII CAGCTG 1 cut(s) 617
RseI CAYNNNNRTG 4 cut(s) 56, 654, 729, 900
Rsr2I CGGWCCG 1 cut(s) 455
RsrII CGGWCCG 1 cut(s) 455
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 3 cut(s) 296, 822, 917
SatI GCNGC 3 cut(s) 167, 391, 615
Sau3AI GATC 1 cut(s) 214
Sau96I GGNCC 4 cut(s) 455, 468, 674, 761
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 4 cut(s) 453, 519, 759, 842
SduI GDGCHC 4 cut(s) 177, 550, 571, 798
SfaNI GCATC 3 cut(s) 128, 333, 721
SinI GGWCC 3 cut(s) 455, 468, 761
SmiMI CAYNNNNRTG 4 cut(s) 56, 654, 729, 900
SphI GCATGC 1 cut(s) 230
Sse9I AATT 4 cut(s) 194, 208, 718, 868
SsiI CCGC 4 cut(s) 162, 458, 663, 704
SspMI CTAG 3 cut(s) 257, 314, 708
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 4 cut(s) 451, 517, 757, 840
StyI CCWWGG 2 cut(s) 424, 649
TaiI ACGT 1 cut(s) 488
TaqI TCGA 2 cut(s) 382, 669
TasI AATT 4 cut(s) 194, 208, 718, 868
TfiI GAWTC 2 cut(s) 782, 889
Tru1I TTAA 3 cut(s) 296, 822, 917
Tru9I TTAA 3 cut(s) 296, 822, 917
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 301, 574
TseI GCWGC 3 cut(s) 166, 390, 614
Tsp45I GTSAC 2 cut(s) 301, 574
TspDTI ATGAA 1 cut(s) 109
TspGWI ACGGA 1 cut(s) 901
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 3 cut(s) 130, 353, 814
VneI GTGCAC 2 cut(s) 546, 794
VpaK11BI GGWCC 3 cut(s) 455, 468, 761
XapI RAATTY 2 cut(s) 194, 718
XbaI TCTAGA 2 cut(s) 256, 313
XceI RCATGY 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 774
XspI CTAG 3 cut(s) 257, 314, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.