Prupe.2G204500_v2.0.a1

Belongs to the peptidase M10A family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0019772
Physical Location & Seq
Reverse (-)
23970051 .. 23970995
945 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G204500.1

Sequence Viewer

Length: 945 bp
ATGCCTGCAATGGCCTCAAAAACCATCTCAGTTTGTGTAGCCTTTTTCCTCATCTTTGTGTTGCTCTCTCAAAACACTCATTCCAAATCTACTCATGACCAGCATGACCCCTTCAAGTTCATCCAACACCTGGAGGGATGCCACAAGGGTCAAAATGTGAGCGGGCTGCAAGAGCTCAAAAAATACCTCACCAAATTTGGCTACCTGAATTATGATCATTCAAAGCATGCCAACGACGAGGAGTTTGATGACATTCTAGAGTCTGCCATCAAGTCATACCAGAAAAGCTATCACTTAAAAGTCACTGGAACTCTAGACACCACCACGGGGAAACAGATGATGATGCCAAGATGTGGTGTGCCGGATGTTGTCAATGGCACACGAAAGGGCAGCAAGAAACACAACCATAAGCACAAGTCTATCCATGGGGTTGCTCATTATGAGTTCTTCTTCCCCGGACCGCGAAGATGGTCCAAAACCCATCTGACGTATCGCTTTAGTTCAAGTGTGAACCAAGTCCCTGGCACACAGAATGTAAGGTCTATATGTGCACAAGCATTTCAGAGATGGGCACAAGTGACCAGTTTTACATTTGAGGAAGTCCCTGCTACTTCAGCAGCTGATATTACCATTGGATTTCACAGGGGCAACCATGGAGATGGTTCCGCATTCGATGGCCCCAGAGGGACTTTCGCGCACGCTAATCCGCCTAGAGGTGGGAATTTCCATTATGATGCAGATGAGAGATGGAGTAGCAATCCAGGACCAAATGAGGTAGACTTGGAATCTGTCGCCGTGCACGAAATAGGCCATCTGCTGGGGCTTAATCACAATCCTGACCTCCCGGACGCAATCATGTATCCCTACTTCAATTATGGGAGTATAAAACGGAATCTGCATAGGGATGACATTGATGGCATACGTGCTTTGTATGGTTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

315

Amino Acids

35.34

Weight (kDa)

8.26

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019772)

Species Orthologous Gene IDs
malus_domestica MD01G1098700.v1.1 MD07G1165400.v1.1
prunus_persica Prupe.2G204200_v2.0.a1 Prupe.2G204300_v2.0.a1 Prupe.2G204500_v2.0.a1
pyrus_communis pycom01g12590 pycom07g16090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 130, 353, 817
AccBSI CCGCTC 1 cut(s) 162
AccI GTMKAC 1 cut(s) 777
AccII CGCG 2 cut(s) 463, 695
AciI CCGC 4 cut(s) 162, 461, 666, 707
AcsI RAATTY 2 cut(s) 194, 721
AcuI CTGAAG 1 cut(s) 597
AfiI CCNNNNNNNGG 6 cut(s) 130, 327, 353, 713, 716, 817
AgsI TTSAA 4 cut(s) 115, 222, 504, 871
AjnI CCWGG 3 cut(s) 129, 520, 760
AluBI AGCT 3 cut(s) 175, 288, 620
AluI AGCT 3 cut(s) 175, 288, 620
Alw21I GWGCWC 3 cut(s) 177, 553, 801
Alw44I GTGCAC 2 cut(s) 549, 797
AlwNI CAGNNNCTG 1 cut(s) 620
AoxI GGCC 3 cut(s) 12, 676, 808
ApaLI GTGCAC 2 cut(s) 549, 797
ApeKI GCWGC 3 cut(s) 166, 390, 617
ApoI RAATTY 2 cut(s) 194, 721
ArsI GACNNNNNNTTYG 2 cut(s) 227, 259
AspLEI GCGC 1 cut(s) 697
AspS9I GGNCC 4 cut(s) 458, 471, 677, 764
AsuC2I CCSGG 2 cut(s) 456, 845
AsuHPI GGTGA 1 cut(s) 181
AvaII GGWCC 3 cut(s) 458, 471, 764
BaeGI GKGCMC 3 cut(s) 553, 574, 801
BanII GRGCYC 1 cut(s) 177
BarI GAAGNNNNNNTAC 2 cut(s) 851, 883
Bbv12I GWGCWC 3 cut(s) 177, 553, 801
BbvI GCAGC 3 cut(s) 153, 402, 629
BceAI ACGGC 1 cut(s) 779
BciT130I CCWGG 3 cut(s) 131, 522, 762
BciVI GTATCC 1 cut(s) 870
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 2 cut(s) 456, 845
BfaI CTAG 3 cut(s) 257, 314, 711
BfuI GTATCC 1 cut(s) 870
BisI GCNGC 3 cut(s) 167, 391, 618
BlsI GCNGC 3 cut(s) 168, 392, 619
Bme1390I CCNGG 5 cut(s) 131, 456, 522, 762, 845
Bme18I GGWCC 3 cut(s) 458, 471, 764
BmgT120I GGNCC 4 cut(s) 458, 471, 677, 764
BmiI GGNNCC 2 cut(s) 664, 679
BmrFI CCNGG 5 cut(s) 131, 456, 522, 762, 845
BmsI GCATC 3 cut(s) 128, 333, 724
BpmI CTGGAG 1 cut(s) 152
BpuMI CCSGG 2 cut(s) 456, 845
BsaAI YACGTR 1 cut(s) 923
BsaJI CCNNGG 5 cut(s) 324, 424, 454, 520, 652
Bsc4I CCNNNNNNNGG 6 cut(s) 130, 327, 353, 713, 716, 817
Bse1I ACTGG 2 cut(s) 310, 582
Bse3DI GCAATG 1 cut(s) 15
BseBI CCWGG 3 cut(s) 131, 522, 762
BseDI CCNNGG 5 cut(s) 324, 424, 454, 520, 652
BseGI GGATG 4 cut(s) 120, 143, 370, 910
BseLI CCNNNNNNNGG 6 cut(s) 130, 327, 353, 713, 716, 817
BseMI GCAATG 1 cut(s) 15
BseMII CTCAG 1 cut(s) 42
BseNI ACTGG 2 cut(s) 310, 582
BseRI GAGGAG 1 cut(s) 254
BseSI GKGCMC 3 cut(s) 553, 574, 801
BseXI GCAGC 3 cut(s) 153, 402, 629
BseYI CCCAGC 1 cut(s) 817
Bsh1236I CGCG 2 cut(s) 463, 695
BshFI GGCC 3 cut(s) 14, 678, 810
BsiHKAI GWGCWC 3 cut(s) 177, 553, 801
BsiSI CCGG 3 cut(s) 362, 456, 845
BslFI GGGAC 3 cut(s) 503, 587, 700
BslI CCNNNNNNNGG 6 cut(s) 130, 327, 353, 713, 716, 817
BsmFI GGGAC 3 cut(s) 503, 587, 700
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 3 cut(s) 14, 678, 810
Bsp1286I GDGCHC 4 cut(s) 177, 553, 574, 801
Bsp143I GATC 1 cut(s) 214
Bsp19I CCATGG 2 cut(s) 424, 652
BspACI CCGC 4 cut(s) 162, 461, 666, 707
BspANI GGCC 3 cut(s) 14, 678, 810
BspCNI CTCAG 1 cut(s) 41
BspFNI CGCG 2 cut(s) 463, 695
BspHI TCATGA 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 664, 679
BsrBI CCGCTC 1 cut(s) 162
BsrDI GCAATG 1 cut(s) 15
BsrI ACTGG 2 cut(s) 310, 582
BssECI CCNNGG 5 cut(s) 324, 424, 454, 520, 652
BssMI GATC 1 cut(s) 214
BssT1I CCWWGG 2 cut(s) 424, 652
Bst2UI CCWGG 3 cut(s) 131, 522, 762
Bst4CI ACNGT 1 cut(s) 942
BstBAI YACGTR 1 cut(s) 923
BstC8I GCNNGC 4 cut(s) 6, 164, 228, 699
BstDEI CTNAG 1 cut(s) 28
BstDSI CCRYGG 3 cut(s) 324, 424, 652
BstF5I GGATG 4 cut(s) 120, 143, 370, 910
BstFNI CGCG 2 cut(s) 463, 695
BstHHI GCGC 1 cut(s) 697
BstKTI GATC 1 cut(s) 217
BstMBI GATC 1 cut(s) 214
BstMWI GCNNNNNNNGC 2 cut(s) 172, 614
BstNI CCWGG 3 cut(s) 131, 522, 762
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 5 cut(s) 129, 454, 520, 760, 843
BstSLI GKGCMC 3 cut(s) 553, 574, 801
BstUI CGCG 2 cut(s) 463, 695
BstV1I GCAGC 3 cut(s) 153, 402, 629
BstXI CCANNNNNNTGG 2 cut(s) 521, 659
BsuI GTATCC 1 cut(s) 870
BsuRI GGCC 3 cut(s) 14, 678, 810
BtgI CCRYGG 3 cut(s) 324, 424, 652
BtsCI GGATG 4 cut(s) 120, 143, 370, 910
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 4 cut(s) 6, 164, 228, 699
CaiI CAGNNNCTG 1 cut(s) 620
CciI TCATGA 1 cut(s) 94
CfoI GCGC 1 cut(s) 697
Cfr13I GGNCC 4 cut(s) 458, 471, 677, 764
CpoI CGGWCCG 1 cut(s) 458
CseI GACGC 1 cut(s) 857
CspI CGGWCCG 1 cut(s) 458
CviAII CATG 6 cut(s) 95, 104, 227, 425, 653, 856
DdeI CTNAG 1 cut(s) 28
DpnI GATC 1 cut(s) 216
DpnII GATC 1 cut(s) 214
EciI GGCGGA 1 cut(s) 696
Ecl136II GAGCTC 1 cut(s) 175
Eco130I CCWWGG 2 cut(s) 424, 652
Eco24I GRGCYC 1 cut(s) 177
Eco47I GGWCC 3 cut(s) 458, 471, 764
Eco53kI GAGCTC 1 cut(s) 175
Eco57I CTGAAG 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 175
EcoRII CCWGG 3 cut(s) 129, 520, 760
EcoT14I CCWWGG 2 cut(s) 424, 652
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 2 cut(s) 424, 652
FaeI CATG 6 cut(s) 98, 107, 230, 428, 656, 859
FaqI GGGAC 3 cut(s) 503, 587, 700
FatI CATG 6 cut(s) 94, 103, 226, 424, 652, 855
FauI CCCGC 1 cut(s) 155
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 777
Fnu4HI GCNGC 3 cut(s) 167, 391, 618
FokI GGATG 4 cut(s) 107, 150, 377, 917
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 3 cut(s) 167, 391, 618
FspBI CTAG 3 cut(s) 257, 314, 711
GlaI GCGC 1 cut(s) 696
GluI GCNGC 3 cut(s) 167, 391, 618
GsaI CCCAGC 1 cut(s) 821
GsuI CTGGAG 1 cut(s) 152
HaeIII GGCC 3 cut(s) 14, 678, 810
HapII CCGG 3 cut(s) 362, 456, 845
HgaI GACGC 1 cut(s) 857
HhaI GCGC 1 cut(s) 697
Hin1II CATG 6 cut(s) 98, 107, 230, 428, 656, 859
Hin6I GCGC 1 cut(s) 695
HinP1I GCGC 1 cut(s) 695
HinfI GANTC 3 cut(s) 260, 785, 892
HpaII CCGG 3 cut(s) 362, 456, 845
HphI GGTGA 1 cut(s) 181
Hpy166II GTNNAC 5 cut(s) 511, 551, 778, 799, 938
Hpy188I TCNGA 2 cut(s) 486, 564
Hpy188III TCNNGA 4 cut(s) 95, 257, 314, 836
Hpy8I GTNNAC 5 cut(s) 511, 551, 778, 799, 938
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 1 cut(s) 942
HpyCH4IV ACGT 2 cut(s) 488, 922
HpyCH4V TGCA 6 cut(s) 8, 169, 551, 737, 799, 898
HpyF10VI GCNNNNNNNGC 2 cut(s) 172, 614
HpyF3I CTNAG 1 cut(s) 28
HpySE526I ACGT 2 cut(s) 488, 922
Hsp92II CATG 6 cut(s) 98, 107, 230, 428, 656, 859
HspAI GCGC 1 cut(s) 695
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 1 cut(s) 214
Lsp1109I GCAGC 3 cut(s) 153, 402, 629
LweI GCATC 3 cut(s) 128, 333, 724
MaeI CTAG 3 cut(s) 257, 314, 711
MaeII ACGT 2 cut(s) 488, 922
MaeIII GTNAC 2 cut(s) 301, 577
MalI GATC 1 cut(s) 216
MbiI CCGCTC 1 cut(s) 162
MboI GATC 1 cut(s) 214
MboII GAAGA 3 cut(s) 439, 442, 477
MhlI GDGCHC 4 cut(s) 177, 553, 574, 801
MluCI AATT 4 cut(s) 194, 208, 721, 871
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 148
MseI TTAA 2 cut(s) 296, 825
MslI CAYNNNNRTG 4 cut(s) 56, 657, 732, 903
MspA1I CMGCKG 1 cut(s) 620
MspI CCGG 3 cut(s) 362, 456, 845
MspR9I CCNGG 5 cut(s) 131, 456, 522, 762, 845
Mva1269I GAATGC 1 cut(s) 668
MvaI CCWGG 3 cut(s) 131, 522, 762
MvnI CGCG 2 cut(s) 463, 695
MwoI GCNNNNNNNGC 2 cut(s) 172, 614
NciI CCSGG 2 cut(s) 456, 845
NcoI CCATGG 2 cut(s) 424, 652
NdeII GATC 1 cut(s) 214
NlaIII CATG 6 cut(s) 98, 107, 230, 428, 656, 859
NlaIV GGNNCC 2 cut(s) 664, 679
NmuCI GTSAC 2 cut(s) 301, 577
NspI RCATGY 1 cut(s) 230
PaeI GCATGC 1 cut(s) 230
PagI TCATGA 1 cut(s) 94
PcsI WCGNNNNNNNCGW 1 cut(s) 798
PctI GAATGC 1 cut(s) 668
PfeI GAWTC 2 cut(s) 785, 892
PflMI CCANNNNNTGG 3 cut(s) 130, 353, 817
PfoI TCCNGGA 2 cut(s) 760, 843
PkrI GCNGC 3 cut(s) 168, 392, 619
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
Ppu21I YACGTR 1 cut(s) 923
Psp124BI GAGCTC 1 cut(s) 177
Psp6I CCWGG 3 cut(s) 129, 520, 760
PspFI CCCAGC 1 cut(s) 817
PspGI CCWGG 3 cut(s) 129, 520, 760
PspN4I GGNNCC 2 cut(s) 664, 679
PspPI GGNCC 4 cut(s) 458, 471, 677, 764
PstNI CAGNNNCTG 1 cut(s) 620
PvuII CAGCTG 1 cut(s) 620
RseI CAYNNNNRTG 4 cut(s) 56, 657, 732, 903
Rsr2I CGGWCCG 1 cut(s) 458
RsrII CGGWCCG 1 cut(s) 458
SacI GAGCTC 1 cut(s) 177
SaqAI TTAA 2 cut(s) 296, 825
SatI GCNGC 3 cut(s) 167, 391, 618
Sau3AI GATC 1 cut(s) 214
Sau96I GGNCC 4 cut(s) 458, 471, 677, 764
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 5 cut(s) 131, 456, 522, 762, 845
SduI GDGCHC 4 cut(s) 177, 553, 574, 801
SfaNI GCATC 3 cut(s) 128, 333, 724
SinI GGWCC 3 cut(s) 458, 471, 764
SmiMI CAYNNNNRTG 4 cut(s) 56, 657, 732, 903
SphI GCATGC 1 cut(s) 230
Sse9I AATT 4 cut(s) 194, 208, 721, 871
SsiI CCGC 4 cut(s) 162, 461, 666, 707
SspMI CTAG 3 cut(s) 257, 314, 711
SstI GAGCTC 1 cut(s) 177
StyD4I CCNGG 5 cut(s) 129, 454, 520, 760, 843
StyI CCWWGG 2 cut(s) 424, 652
TaaI ACNGT 1 cut(s) 942
TaiI ACGT 2 cut(s) 491, 925
TaqI TCGA 1 cut(s) 672
TasI AATT 4 cut(s) 194, 208, 721, 871
TfiI GAWTC 2 cut(s) 785, 892
Tru1I TTAA 2 cut(s) 296, 825
Tru9I TTAA 2 cut(s) 296, 825
TscAI CASTG 1 cut(s) 310
TseFI GTSAC 2 cut(s) 301, 577
TseI GCWGC 3 cut(s) 166, 390, 617
Tsp45I GTSAC 2 cut(s) 301, 577
TspDTI ATGAA 1 cut(s) 109
TspGWI ACGGA 1 cut(s) 904
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 3 cut(s) 130, 353, 817
VneI GTGCAC 2 cut(s) 549, 797
VpaK11BI GGWCC 3 cut(s) 458, 471, 764
XapI RAATTY 2 cut(s) 194, 721
XbaI TCTAGA 2 cut(s) 256, 313
XceI RCATGY 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 777
XspI CTAG 3 cut(s) 257, 314, 711
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.