MD01G1107300.v1.1

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012886
Physical Location & Seq
Forward (+)
22026330 .. 22030735
4406 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1107300.v1.1.491

Sequence Viewer

Length: 2163 bp
ATGGGGAGCAAATGGAGGAAAGCAAAGCTTGCACTGGGGCTGAATCTGTGTGTGTATCTTCCCAAAACACTAGAGGATTCATCTCCCTCCTCTGTGGACTCAGCTGAGAGACTGTCTGATGCAGCCCTGCTTTCACCTGCCAATTGGGCCAATGGCTCCTCCAGGCCCACCACTCCAACCCCATCCTCTCATGGTTTGAAGCTCTCCAGAAGTGGAAGCAAATCCTCAAAGCAAACGTGCTCGATATGCTTAACCAAGATGAAGCAAGGAGGTGGCCAGGCTCTTTTCACCGCCGAATGCTCACATTCTTTTCATTTTCATTGCATCACATCAAACGTGAAACATGGCAACCAAATTTGCCCAGTTTGTAGGGCCAAGTGGAAAGAAATCCCAGTGCAGGGTCCTAGTCTGGATCCTCCTCCTGGAAGGCCAGCTATTGGTCCGGTAGGTTGGCCCCAAAATGATGCTTTGATGACTGTGGTTCGCCGACTACCTCCTCCTCGAAGAGACTTGAGCCGACGGCACATCGTGCCACTGTATCAGGCACACGAGCCGGGTGTATTTAATGATGACGAAACCTTAGATCACCAACCTGTGTTTGATGAGAGAAGCTCTTGCAATAAGAATGGTGGAAACGACAATTTTGTTAGGACGATAGAGATCAAAACATTCCCAGAGGTGACAGCTGTTTCAAAATCCAATTCTCACGACAGTTTTACTGTTTTGGTGCATCTGAAAGCTGCTACTTCAGTTACAAGTCAAAATCCTACCATAAATCTTTCCAGTTTGCCTCAGTTTTCACACAATCATCGTGCTCCAGTAGACCTAGTCACAGTGCTCGACACCAGTGGTAGCATGGCAGGAACCAAGCTTGCATTACTAAAACGGGCTATGGGGTTCGTGATACAGAATCTTGGCTCCAACGACAGACTCTCAGTTATTGCATTCTCTTCAACAGCACGTCGCCTCTTTCCCCTCCGCCGAATGACTGACACTGGACGCCAGCAGGCACTGCAAGCTGTTAACTCTCTGGTTGCAAATGGTGGGACCAACATTGCTGAGGGCTTAAGAAAAGGTGGCAAGGTATTGGAAGATCGAAGGGGAAAGAATCCTGTTGCCAGTATAATACTGTTGTCGGATGGGCAGGACACTTATACTGTCAGTGGCTCTGGGCCTAACCAATCTCAGCCAAATTATCAGTTGCTCCTCCCTTTGTCTATTCATCACAGCGACAATACAGGCTTCCAGATTCCAGTGCATGCATTTGGATTCGGTGCAGATCACGATGCATCATCGATGCACTCGATTTCAGAGATATCTGGGGGAACATTTTCTTTCATCGAGACAGAAGCTGTAATCCAGGATGCATTTGCACAATGCATTGGGGGGCTTTTGAGTGTCGTGGTGCAAGAGCTGCAGGTGGTAGTTGAGTGTGTGAACACAAGTCTCTGCCTTGGTTCAGTAAAAGCAGGAAGCTACCCAAGTCGTGTGATGGGTGATGGACGGAAGGGGGTTATTGATGTTGGAGATATGTATGCCGATGAAGAGAGGGATTTTCTGGTTTCAGTTAATGTCCCAACAGAGTCTTGTAGCAATCTCACATCGTTGATAAAGGTGAGATGCATTTACAAGGATCCCTTGAATAAAGAAACGGCAACAATAGAAAGTGAAGAAGTTAGAATTGAGAGACCTGATGTAGCTCGGCAAGCAGTAGTGTCAATAGAAGTGGACAGGCAACGTAATAGGCTCCAGGCAGCCGAGGCAATGGCTCAAGCACGAGGAGCGGCTGAGCTGGGAGACTTAACCGGTGCAGTTTCAACCCTTGAAAATTGTCGAAGGGTATTGTCAGAGACCGTGTCAGCAAAATCCCAGGACCGCCTGTGCGTAGCATTGGATGCTGAGCTCAAGGAAATGCAAGAGAGGATGGCAAGCAGGCATGTGTACGAGGCATCTGGCAGAGCGTACATCCTTTCAGGATTGAGCTCACACTCATGGCAACGAGCGACAGCAAGAGGTGACTCCACCGATGGTTCAAGTCTTGTTCAGGCCTATCAAACCCCATCAATGGTTGAGATGCTTACTCGATCTCAAGCTATGTTGTTGGGCAGTCCATCAGCCCAGCGGCTTGTCCGACCATTTTATTCACAACCAAAGCCGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

721

Amino Acids

77.97

Weight (kDa)

8.62

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 77 - 124 1.8e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 79 - 124 7.8e-08 Ring finger domain
zf-RING_11 PF17123 80 - 109 1.6e-09 RING-like zinc finger
zf-C3HC4 PF00097 80 - 123 8.4e-08 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 266 - 388 7e-08 VWA domain containing CoxE-like protein
VWA PF00092 275 - 457 3.8e-22 von Willebrand factor type A domain
VWA_3 PF13768 275 - 446 3.4e-18 von Willebrand factor type A domain
VWA_2 PF13519 276 - 382 2.1e-20 von Willebrand factor type A domain
WAV3_C PF25243 576 - 646 6.1e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 625 - 697 8.5e-21 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012886)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38970
fragaria_vesca FvH4_7g19190 FvH4_7g19190
malus_domestica MD01G1107300.v1.1 MD07G1173100.v1.1
pyrus_communis pycom01g13410 pycom07g16770
rosa_chinensis RchiOBHm_Chr1g0363051
rosa_laevigata RLG00000027598
rosa_multiflora Rmu_sc0004472.1_g000004
rosa_roxburghii Rroxscaffold_4G00293520
rosa_rugosa Rorug01G0304000 Rorug01G0304000
rosa_samantha Rh1AG313000 Rh1BG276200 Rh1CG293000 Rh1DG306900
rosa_wichuraiana Rw1G027730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 145, 1408
Acc36I ACCTGC 2 cut(s) 145, 1408
AccB7I CCANNNNNTGG 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 1784
AccI GTMKAC 1 cut(s) 822
AciI CCGC 5 cut(s) 291, 979, 1784, 1876, 2122
AclWI GGATC 4 cut(s) 407, 420, 1628, 1641
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 1 cut(s) 354
AcuI CTGAAG 1 cut(s) 732
AcyI GRCGYC 1 cut(s) 1000
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 2 cut(s) 1943, 1964
AfiI CCNNNNNNNGG 5 cut(s) 397, 398, 422, 437, 2065
AflII CTTAAG 1 cut(s) 1066
AgeI ACCGGT 1 cut(s) 1805
AgsI TTSAA 7 cut(s) 199, 693, 954, 1642, 1818, 1826, 2034
AjiI CACGTC 1 cut(s) 962
AjnI CCWGG 6 cut(s) 161, 276, 421, 1359, 1749, 1869
Alw21I GWGCWC 5 cut(s) 242, 817, 840, 1905, 1985
Alw26I GTCTC 7 cut(s) 103, 501, 1337, 1451, 1681, 1791, 1844
AlwI GGATC 4 cut(s) 407, 420, 1628, 1641
AlwNI CAGNNNCTG 2 cut(s) 1012, 1352
AoxI GGCC 8 cut(s) 147, 164, 274, 372, 428, 452, 1172, 2046
ApeKI GCWGC 4 cut(s) 122, 740, 1414, 1754
ApoI RAATTY 1 cut(s) 354
AsiGI ACCGGT 1 cut(s) 1805
Asp700I GAANNNNTTC 1 cut(s) 1330
AspS9I GGNCC 9 cut(s) 147, 165, 372, 401, 440, 453, 1047, 1172, 1873
AsuC2I CCSGG 1 cut(s) 555
AsuHPI GGTGA 7 cut(s) 126, 280, 578, 691, 1508, 1627, 2027
AvaII GGWCC 4 cut(s) 401, 440, 1047, 1873
BalI TGGCCA 1 cut(s) 276
BamHI GGATCC 2 cut(s) 412, 1633
BanII GRGCYC 2 cut(s) 1905, 1985
BauI CACGAG 2 cut(s) 548, 1776
Bbv12I GWGCWC 5 cut(s) 242, 817, 840, 1905, 1985
BbvCI CCTCAGC 1 cut(s) 1059
BbvI GCAGC 4 cut(s) 134, 727, 1401, 1766
BccI CCATC 8 cut(s) 190, 1133, 1486, 1493, 1918, 2021, 2068, 2119
BceAI ACGGC 2 cut(s) 536, 1668
BciT130I CCWGG 6 cut(s) 163, 278, 423, 1361, 1751, 1871
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 7 cut(s) 103, 501, 1337, 1451, 1681, 1791, 1844
BfaI CTAG 3 cut(s) 71, 405, 827
BfmI CTRYAG 1 cut(s) 1415
BfrI CTTAAG 1 cut(s) 1066
BfuAI ACCTGC 2 cut(s) 145, 1408
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 6 cut(s) 123, 741, 1415, 1755, 1785, 2123
BlpI GCTNAGC 2 cut(s) 1788, 1899
BlsI GCNGC 6 cut(s) 124, 742, 1416, 1756, 1786, 2124
Bme1390I CCNGG 7 cut(s) 163, 278, 423, 555, 1361, 1751, 1871
Bme18I GGWCC 4 cut(s) 401, 440, 1047, 1873
BmgBI CACGTC 1 cut(s) 962
BmgT120I GGNCC 9 cut(s) 147, 165, 372, 401, 440, 453, 1047, 1172, 1873
BmiI GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
BmrFI CCNGG 7 cut(s) 163, 278, 423, 555, 1361, 1751, 1871
BmrI ACTGGG 3 cut(s) 44, 356, 386
BmuI ACTGGG 3 cut(s) 44, 356, 386
BplI GAGNNNNNCTC 2 cut(s) 596, 628
BpmI CTGGAG 4 cut(s) 145, 190, 801, 1733
Bpu10I CCTNAGC 1 cut(s) 1059
Bpu1102I GCTNAGC 2 cut(s) 1788, 1899
BpuEI CTTGAG 4 cut(s) 532, 1755, 1889, 2073
BpuMI CCSGG 1 cut(s) 555
Bsa29I ATCGAT 1 cut(s) 1295
BsaBI GATNNNNATC 1 cut(s) 659
BsaHI GRCGYC 1 cut(s) 1000
BsaI GGTCTC 2 cut(s) 1681, 1844
BsaJI CCNNGG 4 cut(s) 1453, 1758, 1869, 2156
BsaWI WCCGGW 2 cut(s) 442, 1805
Bsc4I CCNNNNNNNGG 5 cut(s) 397, 398, 422, 437, 2065
Bse118I RCCGGY 1 cut(s) 1805
Bse1I ACTGG 9 cut(s) 39, 362, 392, 783, 818, 846, 1000, 1119, 1253
Bse3DI GCAATG 3 cut(s) 319, 1053, 1770
Bse8I GATNNNNATC 1 cut(s) 659
BseBI CCWGG 6 cut(s) 163, 278, 423, 1361, 1751, 1871
BseCI ATCGAT 1 cut(s) 1295
BseDI CCNNGG 4 cut(s) 1453, 1758, 1869, 2156
BseGI GGATG 6 cut(s) 182, 1144, 1369, 1900, 1929, 1965
BseJI GATNNNNATC 1 cut(s) 659
BseLI CCNNNNNNNGG 5 cut(s) 397, 398, 422, 437, 2065
BseMI GCAATG 3 cut(s) 319, 1053, 1770
BseMII CTCAG 8 cut(s) 96, 114, 806, 948, 1050, 1199, 1779, 1890
BseNI ACTGG 9 cut(s) 39, 362, 392, 783, 818, 846, 1000, 1119, 1253
BseRI GAGGAG 7 cut(s) 79, 148, 408, 486, 489, 1196, 1794
BseXI GCAGC 4 cut(s) 134, 727, 1401, 1766
BseYI CCCAGC 2 cut(s) 1792, 2118
BsgI GTGCAG 3 cut(s) 416, 1296, 1830
BshFI GGCC 8 cut(s) 149, 166, 276, 374, 430, 454, 1174, 2048
BshTI ACCGGT 1 cut(s) 1805
BshVI ATCGAT 1 cut(s) 1295
BsiHKAI GWGCWC 5 cut(s) 242, 817, 840, 1905, 1985
BsiSI CCGG 3 cut(s) 443, 554, 1806
BslFI GGGAC 2 cut(s) 1060, 1559
BslI CCNNNNNNNGG 5 cut(s) 397, 398, 422, 437, 2065
BsmAI GTCTC 7 cut(s) 103, 501, 1337, 1451, 1681, 1791, 1844
BsmFI GGGAC 2 cut(s) 1060, 1559
BsmI GAATGC 2 cut(s) 302, 944
BsnI GGCC 8 cut(s) 149, 166, 276, 374, 430, 454, 1174, 2048
Bso31I GGTCTC 2 cut(s) 1681, 1844
Bsp1286I GDGCHC 5 cut(s) 242, 817, 840, 1905, 1985
Bsp143I GATC 7 cut(s) 412, 583, 660, 1093, 1279, 1633, 2084
Bsp1720I GCTNAGC 2 cut(s) 1788, 1899
BspACI CCGC 5 cut(s) 291, 979, 1784, 1876, 2122
BspANI GGCC 8 cut(s) 149, 166, 276, 374, 430, 454, 1174, 2048
BspCNI CTCAG 8 cut(s) 97, 113, 805, 947, 1051, 1198, 1780, 1891
BspDI ATCGAT 1 cut(s) 1295
BspLI GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
BspMAI CTGCAG 1 cut(s) 1419
BspMI ACCTGC 2 cut(s) 145, 1408
BspPI GGATC 4 cut(s) 407, 420, 1628, 1641
BspTI CTTAAG 1 cut(s) 1066
BspTNI GGTCTC 2 cut(s) 1681, 1844
BsrBI CCGCTC 1 cut(s) 1784
BsrDI GCAATG 3 cut(s) 319, 1053, 1770
BsrFI RCCGGY 1 cut(s) 1805
BsrI ACTGG 9 cut(s) 39, 362, 392, 783, 818, 846, 1000, 1119, 1253
BssAI RCCGGY 1 cut(s) 1805
BssECI CCNNGG 4 cut(s) 1453, 1758, 1869, 2156
BssMI GATC 7 cut(s) 412, 583, 660, 1093, 1279, 1633, 2084
BssNI GRCGYC 1 cut(s) 1000
BssSI CACGAG 2 cut(s) 548, 1776
BssT1I CCWWGG 1 cut(s) 1453
Bst2BI CACGAG 2 cut(s) 548, 1776
Bst2UI CCWGG 6 cut(s) 163, 278, 423, 1361, 1751, 1871
Bst4CI ACNGT 9 cut(s) 114, 478, 537, 713, 721, 835, 1131, 1159, 1855
Bst6I CTCTTC 3 cut(s) 499, 955, 1539
BstACI GRCGYC 1 cut(s) 1000
BstAFI CTTAAG 1 cut(s) 1066
BstAPI GCANNNNNTGC 5 cut(s) 29, 529, 1012, 1414, 1895
BstDEI CTNAG 9 cut(s) 100, 105, 580, 792, 934, 1059, 1185, 1788, 1899
BstF5I GGATG 6 cut(s) 182, 1144, 1369, 1900, 1929, 1965
BstKTI GATC 7 cut(s) 415, 586, 663, 1096, 1282, 1636, 2087
BstMAI GTCTC 7 cut(s) 103, 501, 1337, 1451, 1681, 1791, 1844
BstMBI GATC 7 cut(s) 412, 583, 660, 1093, 1279, 1633, 2084
BstNI CCWGG 6 cut(s) 163, 278, 423, 1361, 1751, 1871
BstNSI RCATGY 2 cut(s) 1262, 1940
BstSCI CCNGG 7 cut(s) 161, 276, 421, 553, 1359, 1749, 1869
BstSFI CTRYAG 1 cut(s) 1415
BstV1I GCAGC 4 cut(s) 134, 727, 1401, 1766
BstX2I RGATCY 2 cut(s) 412, 1633
BstYI RGATCY 2 cut(s) 412, 1633
Bsu15I ATCGAT 1 cut(s) 1295
BsuRI GGCC 8 cut(s) 149, 166, 276, 374, 430, 454, 1174, 2048
BsuTUI ATCGAT 1 cut(s) 1295
BtrI CACGTC 1 cut(s) 962
BtsCI GGATG 6 cut(s) 182, 1144, 1369, 1900, 1929, 1965
BtsI GCAGTG 1 cut(s) 1010
BtsIMutI CAGTG 9 cut(s) 32, 399, 533, 840, 853, 993, 1010, 1168, 1260
BveI ACCTGC 2 cut(s) 145, 1408
CaiI CAGNNNCTG 2 cut(s) 1012, 1352
Cfr10I RCCGGY 1 cut(s) 1805
Cfr13I GGNCC 9 cut(s) 147, 165, 372, 401, 440, 453, 1047, 1172, 1873
ClaI ATCGAT 1 cut(s) 1295
CseI GACGC 1 cut(s) 1008
Csp6I GTAC 2 cut(s) 1942, 1963
CspAI ACCGGT 1 cut(s) 1805
CspCI CAANNNNNGTGG 2 cut(s) 1707, 1742
CviAII CATG 6 cut(s) 191, 344, 856, 1259, 1937, 1992
CviQI GTAC 2 cut(s) 1942, 1963
DdeI CTNAG 9 cut(s) 100, 105, 580, 792, 934, 1059, 1185, 1788, 1899
DpnI GATC 7 cut(s) 414, 585, 662, 1095, 1281, 1635, 2086
DpnII GATC 7 cut(s) 412, 583, 660, 1093, 1279, 1633, 2084
DraIII CACNNNGTG 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 3 cut(s) 499, 955, 1539
EarI CTCTTC 3 cut(s) 499, 955, 1539
EciI GGCGGA 1 cut(s) 968
Ecl136II GAGCTC 2 cut(s) 1903, 1983
Eco130I CCWWGG 1 cut(s) 1453
Eco147I AGGCCT 1 cut(s) 2048
Eco24I GRGCYC 2 cut(s) 1905, 1985
Eco31I GGTCTC 2 cut(s) 1681, 1844
Eco32I GATATC 1 cut(s) 1317
Eco47I GGWCC 4 cut(s) 401, 440, 1047, 1873
Eco53kI GAGCTC 2 cut(s) 1903, 1983
Eco57I CTGAAG 1 cut(s) 732
EcoICRI GAGCTC 2 cut(s) 1903, 1983
EcoO109I RGGNCCY 1 cut(s) 401
EcoRII CCWGG 6 cut(s) 161, 276, 421, 1359, 1749, 1869
EcoRV GATATC 1 cut(s) 1317
EcoT14I CCWWGG 1 cut(s) 1453
EcoT22I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
EcoT38I GRGCYC 2 cut(s) 1905, 1985
ErhI CCWWGG 1 cut(s) 1453
FaeI CATG 6 cut(s) 194, 347, 859, 1262, 1940, 1995
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 1622, 1654
FaqI GGGAC 2 cut(s) 1060, 1559
FatI CATG 6 cut(s) 190, 343, 855, 1258, 1936, 1991
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 6 cut(s) 123, 741, 1415, 1755, 1785, 2123
FokI GGATG 6 cut(s) 169, 1151, 1376, 1907, 1936, 1952
FriOI GRGCYC 2 cut(s) 1905, 1985
Fsp4HI GCNGC 6 cut(s) 123, 741, 1415, 1755, 1785, 2123
FspBI CTAG 3 cut(s) 71, 405, 827
GluI GCNGC 6 cut(s) 123, 741, 1415, 1755, 1785, 2123
GsaI CCCAGC 2 cut(s) 1796, 2122
GsuI CTGGAG 4 cut(s) 145, 190, 801, 1733
HaeIII GGCC 8 cut(s) 149, 166, 276, 374, 430, 454, 1174, 2048
HapII CCGG 3 cut(s) 443, 554, 1806
HgaI GACGC 1 cut(s) 1008
Hin1I GRCGYC 1 cut(s) 1000
Hin1II CATG 6 cut(s) 194, 347, 859, 1262, 1940, 1995
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 2 cut(s) 26, 869
HpaI GTTAAC 1 cut(s) 1024
HpaII CCGG 3 cut(s) 443, 554, 1806
HphI GGTGA 7 cut(s) 126, 280, 578, 691, 1508, 1627, 2027
Hpy166II GTNNAC 6 cut(s) 97, 823, 1024, 1438, 1729, 1942
Hpy188I TCNGA 6 cut(s) 118, 735, 1138, 1312, 1849, 2132
Hpy188III TCNNGA 8 cut(s) 207, 410, 707, 901, 1246, 1283, 1342, 1974
Hpy8I GTNNAC 6 cut(s) 97, 823, 1024, 1438, 1729, 1942
Hpy99I CGWCG 2 cut(s) 522, 966
HpyAV CCTTC 4 cut(s) 420, 1092, 1501, 1830
HpyCH4III ACNGT 9 cut(s) 114, 478, 537, 713, 721, 835, 1131, 1159, 1855
HpyCH4IV ACGT 4 cut(s) 236, 336, 961, 1738
HpyF3I CTNAG 9 cut(s) 100, 105, 580, 792, 934, 1059, 1185, 1788, 1899
HpySE526I ACGT 4 cut(s) 236, 336, 961, 1738
Hsp92I GRCGYC 1 cut(s) 1000
Hsp92II CATG 6 cut(s) 194, 347, 859, 1262, 1940, 1995
KspAI GTTAAC 1 cut(s) 1024
Kzo9I GATC 7 cut(s) 412, 583, 660, 1093, 1279, 1633, 2084
LmnI GCTCC 7 cut(s) 6, 161, 820, 923, 1209, 1752, 1781
Lsp1109I GCAGC 4 cut(s) 134, 727, 1401, 1766
MaeI CTAG 3 cut(s) 71, 405, 827
MaeII ACGT 4 cut(s) 236, 336, 961, 1738
MaeIII GTNAC 4 cut(s) 679, 751, 829, 2015
MalI GATC 7 cut(s) 414, 585, 662, 1095, 1281, 1635, 2086
MbiI CCGCTC 1 cut(s) 1784
MboI GATC 7 cut(s) 412, 583, 660, 1093, 1279, 1633, 2084
MboII GAAGA 6 cut(s) 50, 516, 942, 1103, 1556, 1682
MfeI CAATTG 1 cut(s) 142
MflI RGATCY 2 cut(s) 412, 1633
MhlI GDGCHC 5 cut(s) 242, 817, 840, 1905, 1985
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 7 cut(s) 142, 354, 640, 700, 1192, 1680, 1828
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 4 cut(s) 92, 924, 1592, 2012
MmeI TCCRAC 5 cut(s) 200, 945, 1116, 1504, 2155
Mox20I TGGCCA 1 cut(s) 276
Mph1103I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
MroXI GAANNNNTTC 1 cut(s) 1330
MscI TGGCCA 1 cut(s) 276
MseI TTAA 6 cut(s) 251, 564, 1023, 1067, 1569, 1802
MslI CAYNNNNRTG 1 cut(s) 1990
Msp20I TGGCCA 1 cut(s) 276
MspA1I CMGCKG 3 cut(s) 104, 686, 2122
MspCI CTTAAG 1 cut(s) 1066
MspI CCGG 3 cut(s) 443, 554, 1806
MspR9I CCNGG 7 cut(s) 163, 278, 423, 555, 1361, 1751, 1871
MunI CAATTG 1 cut(s) 142
Mva1269I GAATGC 2 cut(s) 302, 944
MvaI CCWGG 6 cut(s) 163, 278, 423, 1361, 1751, 1871
NciI CCSGG 1 cut(s) 555
NdeII GATC 7 cut(s) 412, 583, 660, 1093, 1279, 1633, 2084
NlaIII CATG 6 cut(s) 194, 347, 859, 1262, 1940, 1995
NlaIV GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
NmeAIII GCCGAG 2 cut(s) 1681, 1783
NmuCI GTSAC 3 cut(s) 679, 829, 2015
NsiI ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
NspI RCATGY 2 cut(s) 1262, 1940
PaeI GCATGC 1 cut(s) 1262
PaqCI CACCTGC 2 cut(s) 145, 1408
PceI AGGCCT 1 cut(s) 2048
PcsI WCGNNNNNNNCGW 1 cut(s) 1301
PctI GAATGC 2 cut(s) 302, 944
PdmI GAANNNNTTC 1 cut(s) 1330
PfeI GAWTC 6 cut(s) 43, 77, 910, 1108, 1249, 1269
PflFI GACNNNGTC 2 cut(s) 827, 1855
PflMI CCANNNNNTGG 1 cut(s) 437
PfoI TCCNGGA 2 cut(s) 421, 1359
PinAI ACCGGT 1 cut(s) 1805
PkrI GCNGC 6 cut(s) 124, 742, 1416, 1756, 1786, 2124
PleI GAGTC 4 cut(s) 92, 924, 1591, 2012
PpsI GAGTC 4 cut(s) 92, 924, 1591, 2012
PpuMI RGGWCCY 1 cut(s) 401
Psp124BI GAGCTC 2 cut(s) 1905, 1985
Psp5II RGGWCCY 1 cut(s) 401
Psp6I CCWGG 6 cut(s) 161, 276, 421, 1359, 1749, 1869
PspFI CCCAGC 2 cut(s) 1792, 2118
PspGI CCWGG 6 cut(s) 161, 276, 421, 1359, 1749, 1869
PspN4I GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
PspPI GGNCC 9 cut(s) 147, 165, 372, 401, 440, 453, 1047, 1172, 1873
PspPPI RGGWCCY 1 cut(s) 401
PstI CTGCAG 1 cut(s) 1419
PstNI CAGNNNCTG 2 cut(s) 1012, 1352
PsuI RGATCY 2 cut(s) 412, 1633
PsyI GACNNNGTC 2 cut(s) 827, 1855
PvuII CAGCTG 2 cut(s) 104, 686
RsaI GTAC 2 cut(s) 1943, 1964
RsaNI GTAC 2 cut(s) 1942, 1963
RseI CAYNNNNRTG 1 cut(s) 1990
SacI GAGCTC 2 cut(s) 1905, 1985
SaqAI TTAA 6 cut(s) 251, 564, 1023, 1067, 1569, 1802
SatI GCNGC 6 cut(s) 123, 741, 1415, 1755, 1785, 2123
Sau3AI GATC 7 cut(s) 412, 583, 660, 1093, 1279, 1633, 2084
Sau96I GGNCC 9 cut(s) 147, 165, 372, 401, 440, 453, 1047, 1172, 1873
SchI GAGTC 4 cut(s) 92, 924, 1592, 2012
ScrFI CCNGG 7 cut(s) 163, 278, 423, 555, 1361, 1751, 1871
SduI GDGCHC 5 cut(s) 242, 817, 840, 1905, 1985
SfcI CTRYAG 1 cut(s) 1415
SinI GGWCC 4 cut(s) 401, 440, 1047, 1873
SmiMI CAYNNNNRTG 1 cut(s) 1990
SmlI CTYRAG 5 cut(s) 511, 1066, 1770, 1904, 2088
SmoI CTYRAG 5 cut(s) 511, 1066, 1770, 1904, 2088
SphI GCATGC 1 cut(s) 1262
Sse9I AATT 7 cut(s) 142, 354, 640, 700, 1192, 1680, 1828
SseBI AGGCCT 1 cut(s) 2048
SsiI CCGC 5 cut(s) 291, 979, 1784, 1876, 2122
SspMI CTAG 3 cut(s) 71, 405, 827
SstI GAGCTC 2 cut(s) 1905, 1985
StuI AGGCCT 1 cut(s) 2048
StyD4I CCNGG 7 cut(s) 161, 276, 421, 553, 1359, 1749, 1869
StyI CCWWGG 1 cut(s) 1453
TaaI ACNGT 9 cut(s) 114, 478, 537, 713, 721, 835, 1131, 1159, 1855
TaiI ACGT 4 cut(s) 239, 339, 964, 1741
TaqI TCGA 9 cut(s) 242, 502, 840, 1096, 1295, 1304, 1341, 1834, 2083
TasI AATT 7 cut(s) 142, 354, 640, 700, 1192, 1680, 1828
TauI GCSGC 2 cut(s) 1787, 2125
TfiI GAWTC 6 cut(s) 43, 77, 910, 1108, 1249, 1269
Tru1I TTAA 6 cut(s) 251, 564, 1023, 1067, 1569, 1802
Tru9I TTAA 6 cut(s) 251, 564, 1023, 1067, 1569, 1802
TscAI CASTG 9 cut(s) 39, 399, 540, 840, 853, 1000, 1017, 1168, 1260
TseFI GTSAC 3 cut(s) 679, 829, 2015
TseI GCWGC 4 cut(s) 122, 740, 1414, 1754
Tsp45I GTSAC 3 cut(s) 679, 829, 2015
TspDTI ATGAA 7 cut(s) 69, 275, 302, 308, 1211, 1327, 1557
TspGWI ACGGA 1 cut(s) 1519
TspRI CASTG 9 cut(s) 39, 399, 540, 840, 853, 1000, 1017, 1168, 1260
Tth111I GACNNNGTC 2 cut(s) 827, 1855
Van91I CCANNNNNTGG 1 cut(s) 437
Vha464I CTTAAG 1 cut(s) 1066
VpaK11BI GGWCC 4 cut(s) 401, 440, 1047, 1873
XapI RAATTY 1 cut(s) 354
XceI RCATGY 2 cut(s) 1262, 1940
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 1 cut(s) 1330
XspI CTAG 3 cut(s) 71, 405, 827
Zsp2I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.