pycom07g16770

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012886
Physical Location & Seq
Forward (+)
19225978 .. 19229584
3607 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g16770.2

Sequence Viewer

Length: 2160 bp
ATGGGGAGCAAATGGAGGAAAGCAAAGCTTGCACTGGGGCTGAATCTGTGTTTATATCTTCCCAAAACACTAGAGGATTCATCTCCCTCCTCTGTGGACTCAGCTGAGAGGTTGTCTGATGCTGCCTTGCTTTCACCTGCCAATTGGGCCAATGGCTCCTCCAGGCCTACCACTCCAACTCCATCCTCTCATGGTTTGAACCTCTCCAGAAATGGAAGCAAATCCTCAAAGCAAACGTGCTCGATATGCTTAACCAAAATGAAACAAGGAGGTGGCCATGCTCTTTTCACTGCTGAATGTTCACATTCTTTTCATTTTCACTGCATCACATCAAACGTGAAACACGGCAACCAAATTTGCCCAGTTTGTAGGGCAAAGTGGAAAGAAATCCCAGAGCAGGGTCCTAGTTTGGATCCTCCCCCTGGAAGGGCAGCTATTGGTCCGGTAGGTTGGCCCCAAAATGATGCTTTGATGACTGTGGTTCGCCGACTTCCTCCTCCTCGAAGACACTTGAGCCGACCACACATCATGCCACTTTACCAGGCCCATGAGCCAGCTGTATTTAATGATGATGAATCCTTAGACCAACCTGTGTTTGCTGAGAGAAGCTTGTGCGATAAAAATGGTGCAGATGACAATTTGGTTAGGACGATAGAGATCAAAACATTCCCAGAGGTTTCAGCTGTTTCAAAATCCAATTCTTGCGGCAATTTTACAGTTTTGATCCATCTGAAAGCGGCTACTTCAATTACAAGACAAAATCCTAGCAGAAATCAGTCCACTTTGCCTCCTCAGTTTTCTCAGAATCATCGTGCTCCAGTAGACCTAGTCACAGTGCTTGACACCAGTGGTAGCATGGCAGGAACCAAACTTGCATTGCTAAAAAGGGCTATGGGGTTCGTAATACAGAATCTTGGCTCCAATGACAGACTCTCGGTTATTTCATTCTCTTCAACAGCACGTCGCCTCTTTCCCCTCCGCCGGATGACTGATGCTGGACGCCAGCAGGCATTGCAAGCTGTTAATTCTCTGGTCGCAAATGGTGGGACCAACATTGCCGAGGGCCTAAGGAAGGGTGGCAAGGTATTGGAAGATCGAACGGGAAAGAATCCTGTTGCCAGTATAATACTGTTGTCAGATGGGCAGGACACTTATACTGTCAGTGGCTCTGGGCCTAACCAATCTCAGCCAAATTATAAGTTGCTCCTCCCTTTGTCTATTCATGGTGGTGACAATACAGGCTTCCAGATTCCAGTGCATGCATTTGGATTTGGTGCAGATCATGATGCATCATCGATGCACTCGATTTCAGAGATATCCGGGGGAACATTTTCTTTCATTGAGACAGAAGCTGTCATCCAGGATGCATTTGCACAATGCATTGGGGGGCTTTTGAGTGTTGTGGTGCAAGAGCTGCAGGTGGCAGTTGAGTGTGTGAACACAAGTCTCCGCCTCGGTTCACTAAAAGCAGGAAGCTACCCAAGTTGTGTGATGGCTGATGGGCAGAAAGGGGTTATTGATGTTGGAGGTATGTATGCTGATGAAGAGAGGGATTTTCTGGTTTCAGTAAATGTCCCAACAGAGTCTTGTAGCAATGTAACGTCATTGATAAAGGTGAAATGCATTTACAAGGATCCCTTAAATAAAGAAATGGCGACAATAGAGAGTGAAGAAGTTAGAATTGAGAGACCTGATGTAGTTGGGCAAGCAGTAGTGTCAATAGAAGTGGACAGGCAACGTAATAGGCTCCAGGCGGCCGAGGCAATGGCTCAAGCGCGAGCTGCAGCTGAGCTGGGAGACTTAGCTGGTGCAGTTTCAACCCTTGAACATTGTCGAAGGGTATTGTCAGACACCGTGTCAGCAAAATCCCAAGACCGTCTGTGTGTAGCATTGGATGCTGAGCTCAAGGAAATGCAAGAGAGGATGGCAAGCAGGCAGGTGTACGAGGCATCTGGCAGAGCATACATCCTTTCGGGATTAAGCTCACACTCATGGCAACGAGCGACAGCTAGAGGTGACTCTACTGATGGTTCGAGTCTTGTTCAGGCTTATCAAACCCCATCAATGGTCGAGATGCTTACTAGATCTCAAGCTACATTGTTGGGCAGTCCATCAGCCCAGCGGCTAGTCCGACCAACCAAGGCCGAGGTAATCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

720

Amino Acids

77.35

Weight (kDa)

8.25

Isoelectric Point (pI)

49.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012886)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38970
fragaria_vesca FvH4_7g19190 FvH4_7g19190
malus_domestica MD01G1107300.v1.1 MD07G1173100.v1.1
pyrus_communis pycom01g13410 pycom07g16770
rosa_chinensis RchiOBHm_Chr1g0363051
rosa_laevigata RLG00000027598
rosa_multiflora Rmu_sc0004472.1_g000004
rosa_roxburghii Rroxscaffold_4G00293520
rosa_rugosa Rorug01G0304000 Rorug01G0304000
rosa_samantha Rh1AG313000 Rh1BG276200 Rh1CG293000 Rh1DG306900
rosa_wichuraiana Rw1G027730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1197
AarI CACCTGC 3 cut(s) 145, 1408, 1927
Acc36I ACCTGC 3 cut(s) 145, 1408, 1927
AccI GTMKAC 1 cut(s) 822
AccII CGCG 1 cut(s) 1777
AciI CCGC 6 cut(s) 705, 737, 979, 1450, 1754, 2122
AclWI GGATC 5 cut(s) 407, 420, 718, 1628, 1641
AcoI YGGCCR 2 cut(s) 274, 1755
AcsI RAATTY 1 cut(s) 354
AcyI GRCGYC 1 cut(s) 1000
AfaI GTAC 1 cut(s) 1943
AfiI CCNNNNNNNGG 8 cut(s) 397, 398, 422, 426, 427, 981, 1072, 2065
AgsI TTSAA 6 cut(s) 199, 690, 747, 954, 1818, 1826
AhdI GACNNNNNGTC 1 cut(s) 1855
AjiI CACGTC 1 cut(s) 962
AjnI CCWGG 5 cut(s) 161, 421, 540, 1359, 1749
Alw21I GWGCWC 3 cut(s) 242, 817, 1905
Alw26I GTCTC 4 cut(s) 1337, 1451, 1681, 1791
AlwI GGATC 5 cut(s) 407, 420, 718, 1628, 1641
AlwNI CAGNNNCTG 1 cut(s) 1352
AoxI GGCC 9 cut(s) 147, 164, 274, 452, 543, 1063, 1172, 1755, 2142
ApeKI GCWGC 5 cut(s) 122, 431, 1414, 1781, 1784
ApoI RAATTY 1 cut(s) 354
Asp700I GAANNNNTTC 1 cut(s) 1330
AspLEI GCGC 1 cut(s) 1777
AspS9I GGNCC 8 cut(s) 147, 401, 440, 453, 544, 1047, 1063, 1172
AsuC2I CCSGG 1 cut(s) 1321
AsuHPI GGTGA 4 cut(s) 126, 1241, 1627, 2027
AvaII GGWCC 3 cut(s) 401, 440, 1047
AxyI CCTNAGG 1 cut(s) 1067
BalI TGGCCA 1 cut(s) 276
BamHI GGATCC 2 cut(s) 412, 1633
BanII GRGCYC 1 cut(s) 1905
BbsI GAAGAC 1 cut(s) 511
Bbv12I GWGCWC 3 cut(s) 242, 817, 1905
BbvI GCAGC 5 cut(s) 109, 443, 1401, 1768, 1796
BccI CCATC 9 cut(s) 190, 735, 1133, 1486, 1493, 1918, 2021, 2068, 2119
BceAI ACGGC 1 cut(s) 361
BciT130I CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
BcnI CCSGG 1 cut(s) 1321
BcoDI GTCTC 4 cut(s) 1337, 1451, 1681, 1791
BfaI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
BfmI CTRYAG 2 cut(s) 1415, 1782
BfuAI ACCTGC 3 cut(s) 145, 1408, 1927
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 1 cut(s) 2084
BisI GCNGC 9 cut(s) 123, 432, 706, 738, 1415, 1755, 1782, 1785, 2123
BlpI GCTNAGC 2 cut(s) 1788, 1899
BlsI GCNGC 9 cut(s) 124, 433, 707, 739, 1416, 1756, 1783, 1786, 2124
Bme1390I CCNGG 6 cut(s) 163, 423, 542, 1321, 1361, 1751
Bme18I GGWCC 3 cut(s) 401, 440, 1047
BmeRI GACNNNNNGTC 1 cut(s) 1855
BmgBI CACGTC 1 cut(s) 962
BmgT120I GGNCC 8 cut(s) 147, 401, 440, 453, 544, 1047, 1063, 1172
BmiI GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
BmrFI CCNGG 6 cut(s) 163, 423, 542, 1321, 1361, 1751
BmrI ACTGGG 2 cut(s) 44, 356
BmuI ACTGGG 2 cut(s) 44, 356
BpiI GAAGAC 1 cut(s) 511
BpmI CTGGAG 4 cut(s) 145, 190, 801, 1733
Bpu1102I GCTNAGC 2 cut(s) 1788, 1899
BpuEI CTTGAG 4 cut(s) 532, 1755, 1889, 2073
BpuMI CCSGG 1 cut(s) 1321
Bsa29I ATCGAT 1 cut(s) 1295
BsaBI GATNNNNATC 1 cut(s) 656
BsaHI GRCGYC 1 cut(s) 1000
BsaI GGTCTC 1 cut(s) 1681
BsaJI CCNNGG 7 cut(s) 421, 1059, 1320, 1453, 1758, 2139, 2145
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 4 cut(s) 163, 193, 772, 802
Bsc4I CCNNNNNNNGG 8 cut(s) 397, 398, 422, 426, 427, 981, 1072, 2065
Bse1I ACTGG 6 cut(s) 39, 362, 818, 846, 1119, 1253
Bse21I CCTNAGG 1 cut(s) 1067
Bse3DI GCAATG 5 cut(s) 875, 1010, 1053, 1600, 1770
Bse8I GATNNNNATC 1 cut(s) 656
BseBI CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
BseCI ATCGAT 1 cut(s) 1295
BseDI CCNNGG 7 cut(s) 421, 1059, 1320, 1453, 1758, 2139, 2145
BseGI GGATG 7 cut(s) 182, 990, 1356, 1369, 1900, 1929, 1965
BseJI GATNNNNATC 1 cut(s) 656
BseLI CCNNNNNNNGG 8 cut(s) 397, 398, 422, 426, 427, 981, 1072, 2065
BseMI GCAATG 5 cut(s) 875, 1010, 1053, 1600, 1770
BseMII CTCAG 8 cut(s) 96, 114, 591, 806, 815, 1199, 1779, 1890
BseNI ACTGG 6 cut(s) 39, 362, 818, 846, 1119, 1253
BseRI GAGGAG 6 cut(s) 79, 148, 486, 489, 780, 1196
BseX3I CGGCCG 1 cut(s) 1755
BseXI GCAGC 5 cut(s) 109, 443, 1401, 1768, 1796
BseYI CCCAGC 2 cut(s) 1792, 2118
BsgI GTGCAG 3 cut(s) 648, 1296, 1830
Bsh1236I CGCG 1 cut(s) 1777
Bsh1285I CGRYCG 1 cut(s) 1758
BshFI GGCC 9 cut(s) 149, 166, 276, 454, 545, 1065, 1174, 1757, 2144
BshVI ATCGAT 1 cut(s) 1295
BsiEI CGRYCG 1 cut(s) 1758
BsiHKAI GWGCWC 3 cut(s) 242, 817, 1905
BsiSI CCGG 3 cut(s) 443, 982, 1320
BslFI GGGAC 2 cut(s) 1060, 1559
BslI CCNNNNNNNGG 8 cut(s) 397, 398, 422, 426, 427, 981, 1072, 2065
BsmAI GTCTC 4 cut(s) 1337, 1451, 1681, 1791
BsmFI GGGAC 2 cut(s) 1060, 1559
BsnI GGCC 9 cut(s) 149, 166, 276, 454, 545, 1065, 1174, 1757, 2144
Bso31I GGTCTC 1 cut(s) 1681
Bsp1286I GDGCHC 3 cut(s) 242, 817, 1905
Bsp143I GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
Bsp1720I GCTNAGC 2 cut(s) 1788, 1899
BspACI CCGC 6 cut(s) 705, 737, 979, 1450, 1754, 2122
BspANI GGCC 9 cut(s) 149, 166, 276, 454, 545, 1065, 1174, 1757, 2144
BspCNI CTCAG 8 cut(s) 97, 113, 592, 805, 814, 1198, 1780, 1891
BspDI ATCGAT 1 cut(s) 1295
BspFNI CGCG 1 cut(s) 1777
BspHI TCATGA 1 cut(s) 1282
BspLI GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
BspMAI CTGCAG 2 cut(s) 1419, 1786
BspMI ACCTGC 3 cut(s) 145, 1408, 1927
BspPI GGATC 5 cut(s) 407, 420, 718, 1628, 1641
BspTNI GGTCTC 1 cut(s) 1681
BsrDI GCAATG 5 cut(s) 875, 1010, 1053, 1600, 1770
BsrI ACTGG 6 cut(s) 39, 362, 818, 846, 1119, 1253
BssECI CCNNGG 7 cut(s) 421, 1059, 1320, 1453, 1758, 2139, 2145
BssMI GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
BssNI GRCGYC 1 cut(s) 1000
BssT1I CCWWGG 1 cut(s) 2139
Bst2UI CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
Bst4CI ACNGT 7 cut(s) 478, 718, 835, 1131, 1159, 1855, 1877
Bst6I CTCTTC 2 cut(s) 955, 1539
BstACI GRCGYC 1 cut(s) 1000
BstAPI GCANNNNNTGC 4 cut(s) 29, 1012, 1414, 1895
BstENI CCTNNNNNAGG 1 cut(s) 1070
BstF5I GGATG 7 cut(s) 182, 990, 1356, 1369, 1900, 1929, 1965
BstFNI CGCG 1 cut(s) 1777
BstHHI GCGC 1 cut(s) 1777
BstKTI GATC 7 cut(s) 415, 660, 726, 1096, 1282, 1636, 2087
BstMAI GTCTC 4 cut(s) 1337, 1451, 1681, 1791
BstMBI GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
BstMCI CGRYCG 1 cut(s) 1758
BstMWI GCNNNNNNNGC 9 cut(s) 29, 146, 246, 1012, 1016, 1414, 1760, 1781, 1895
BstNI CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
BstNSI RCATGY 1 cut(s) 1262
BstSCI CCNGG 6 cut(s) 161, 421, 540, 1319, 1359, 1749
BstSFI CTRYAG 2 cut(s) 1415, 1782
BstUI CGCG 1 cut(s) 1777
BstV1I GCAGC 5 cut(s) 109, 443, 1401, 1768, 1796
BstV2I GAAGAC 1 cut(s) 511
BstX2I RGATCY 3 cut(s) 412, 1633, 2084
BstYI RGATCY 3 cut(s) 412, 1633, 2084
BstZI CGGCCG 1 cut(s) 1755
Bsu15I ATCGAT 1 cut(s) 1295
Bsu36I CCTNAGG 1 cut(s) 1067
BsuRI GGCC 9 cut(s) 149, 166, 276, 454, 545, 1065, 1174, 1757, 2144
BsuTUI ATCGAT 1 cut(s) 1295
BtrI CACGTC 1 cut(s) 962
BtsCI GGATG 7 cut(s) 182, 990, 1356, 1369, 1900, 1929, 1965
BtsI GCAGTG 2 cut(s) 288, 319
BtsIMutI CAGTG 7 cut(s) 32, 288, 319, 840, 853, 1168, 1260
BveI ACCTGC 3 cut(s) 145, 1408, 1927
CaiI CAGNNNCTG 1 cut(s) 1352
CciI TCATGA 1 cut(s) 1282
CfoI GCGC 1 cut(s) 1777
Cfr13I GGNCC 8 cut(s) 147, 401, 440, 453, 544, 1047, 1063, 1172
ClaI ATCGAT 1 cut(s) 1295
CseI GACGC 1 cut(s) 1008
Csp6I GTAC 1 cut(s) 1942
CspCI CAANNNNNGTGG 2 cut(s) 1707, 1742
CviAII CATG 9 cut(s) 191, 278, 529, 548, 856, 1223, 1259, 1283, 1992
CviQI GTAC 1 cut(s) 1942
DpnI GATC 7 cut(s) 414, 659, 725, 1095, 1281, 1635, 2086
DpnII GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
DriI GACNNNNNGTC 1 cut(s) 1855
EaeI YGGCCR 2 cut(s) 274, 1755
EagI CGGCCG 1 cut(s) 1755
Eam1104I CTCTTC 2 cut(s) 955, 1539
Eam1105I GACNNNNNGTC 1 cut(s) 1855
EarI CTCTTC 2 cut(s) 955, 1539
EciI GGCGGA 2 cut(s) 968, 1439
Ecl136II GAGCTC 1 cut(s) 1903
EclXI CGGCCG 1 cut(s) 1755
Eco130I CCWWGG 1 cut(s) 2139
Eco147I AGGCCT 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 1905
Eco31I GGTCTC 1 cut(s) 1681
Eco32I GATATC 1 cut(s) 1317
Eco47I GGWCC 3 cut(s) 401, 440, 1047
Eco52I CGGCCG 1 cut(s) 1755
Eco53kI GAGCTC 1 cut(s) 1903
Eco81I CCTNAGG 1 cut(s) 1067
EcoICRI GAGCTC 1 cut(s) 1903
EcoNI CCTNNNNNAGG 1 cut(s) 1070
EcoO109I RGGNCCY 2 cut(s) 401, 1063
EcoRII CCWGG 5 cut(s) 161, 421, 540, 1359, 1749
EcoRV GATATC 1 cut(s) 1317
EcoT14I CCWWGG 1 cut(s) 2139
EcoT22I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
EcoT38I GRGCYC 1 cut(s) 1905
ErhI CCWWGG 1 cut(s) 2139
FaeI CATG 9 cut(s) 194, 281, 532, 551, 859, 1226, 1262, 1286, 1995
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 1622, 1654
FaqI GGGAC 2 cut(s) 1060, 1559
FatI CATG 9 cut(s) 190, 277, 528, 547, 855, 1222, 1258, 1282, 1991
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 9 cut(s) 123, 432, 706, 738, 1415, 1755, 1782, 1785, 2123
FokI GGATG 7 cut(s) 169, 997, 1343, 1376, 1907, 1936, 1952
FriOI GRGCYC 1 cut(s) 1905
Fsp4HI GCNGC 9 cut(s) 123, 432, 706, 738, 1415, 1755, 1782, 1785, 2123
FspBI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
GlaI GCGC 1 cut(s) 1776
GluI GCNGC 9 cut(s) 123, 432, 706, 738, 1415, 1755, 1782, 1785, 2123
GsaI CCCAGC 2 cut(s) 1796, 2122
GsuI CTGGAG 4 cut(s) 145, 190, 801, 1733
HaeIII GGCC 9 cut(s) 149, 166, 276, 454, 545, 1065, 1174, 1757, 2144
HapII CCGG 3 cut(s) 443, 982, 1320
HgaI GACGC 1 cut(s) 1008
HhaI GCGC 1 cut(s) 1777
Hin1I GRCGYC 1 cut(s) 1000
Hin1II CATG 9 cut(s) 194, 281, 532, 551, 859, 1226, 1262, 1286, 1995
Hin6I GCGC 1 cut(s) 1775
HinP1I GCGC 1 cut(s) 1775
HindIII AAGCTT 2 cut(s) 26, 607
HpaII CCGG 3 cut(s) 443, 982, 1320
HphI GGTGA 4 cut(s) 126, 1241, 1627, 2027
Hpy166II GTNNAC 8 cut(s) 97, 302, 780, 823, 1438, 1460, 1729, 1942
Hpy188I TCNGA 7 cut(s) 118, 732, 804, 1138, 1312, 1849, 2132
Hpy188III TCNNGA 5 cut(s) 207, 1246, 1283, 1974, 2071
Hpy8I GTNNAC 8 cut(s) 97, 302, 780, 823, 1438, 1460, 1729, 1942
Hpy99I CGWCG 1 cut(s) 966
HpyAV CCTTC 3 cut(s) 420, 1066, 1830
HpyCH4III ACNGT 7 cut(s) 478, 718, 835, 1131, 1159, 1855, 1877
HpyCH4IV ACGT 5 cut(s) 236, 336, 961, 1601, 1738
HpyF10VI GCNNNNNNNGC 9 cut(s) 29, 146, 246, 1012, 1016, 1414, 1760, 1781, 1895
HpySE526I ACGT 5 cut(s) 236, 336, 961, 1601, 1738
Hsp92I GRCGYC 1 cut(s) 1000
Hsp92II CATG 9 cut(s) 194, 281, 532, 551, 859, 1226, 1262, 1286, 1995
HspAI GCGC 1 cut(s) 1775
Kzo9I GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
LmnI GCTCC 6 cut(s) 6, 161, 820, 923, 1209, 1752
Lsp1109I GCAGC 5 cut(s) 109, 443, 1401, 1768, 1796
MaeI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
MaeII ACGT 5 cut(s) 236, 336, 961, 1601, 1738
MaeIII GTNAC 4 cut(s) 829, 1229, 1597, 2015
MalI GATC 7 cut(s) 414, 659, 725, 1095, 1281, 1635, 2086
MboI GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
MboII GAAGA 6 cut(s) 50, 516, 942, 1103, 1556, 1682
MfeI CAATTG 1 cut(s) 142
MflI RGATCY 3 cut(s) 412, 1633, 2084
MhlI GDGCHC 3 cut(s) 242, 817, 1905
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 9 cut(s) 142, 354, 637, 697, 709, 747, 1024, 1192, 1680
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 5 cut(s) 92, 924, 1592, 2012, 2044
MmeI TCCRAC 3 cut(s) 200, 1504, 2155
Mox20I TGGCCA 1 cut(s) 276
Mph1103I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
MroXI GAANNNNTTC 1 cut(s) 1330
MscI TGGCCA 1 cut(s) 276
MseI TTAA 5 cut(s) 251, 564, 1023, 1640, 1979
MslI CAYNNNNRTG 2 cut(s) 1227, 1990
Msp20I TGGCCA 1 cut(s) 276
MspA1I CMGCKG 5 cut(s) 104, 557, 683, 1787, 2122
MspI CCGG 3 cut(s) 443, 982, 1320
MspR9I CCNGG 6 cut(s) 163, 423, 542, 1321, 1361, 1751
MunI CAATTG 1 cut(s) 142
MvaI CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
MvnI CGCG 1 cut(s) 1777
MwoI GCNNNNNNNGC 9 cut(s) 29, 146, 246, 1012, 1016, 1414, 1760, 1781, 1895
NciI CCSGG 1 cut(s) 1321
NdeII GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
NlaIII CATG 9 cut(s) 194, 281, 532, 551, 859, 1226, 1262, 1286, 1995
NlaIV GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
NmeAIII GCCGAG 2 cut(s) 1084, 1783
NmuCI GTSAC 3 cut(s) 829, 1229, 2015
NsiI ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
NspI RCATGY 1 cut(s) 1262
PaeI GCATGC 1 cut(s) 1262
PagI TCATGA 1 cut(s) 1282
PaqCI CACCTGC 3 cut(s) 145, 1408, 1927
PceI AGGCCT 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 1301
PdmI GAANNNNTTC 1 cut(s) 1330
PfeI GAWTC 7 cut(s) 43, 77, 575, 805, 910, 1108, 1249
PflFI GACNNNGTC 1 cut(s) 827
PfoI TCCNGGA 1 cut(s) 1359
PkrI GCNGC 9 cut(s) 124, 433, 707, 739, 1416, 1756, 1783, 1786, 2124
PleI GAGTC 5 cut(s) 92, 924, 1591, 2012, 2043
PpsI GAGTC 5 cut(s) 92, 924, 1591, 2012, 2043
PpuMI RGGWCCY 1 cut(s) 401
PsiI TTATAA 1 cut(s) 1197
Psp124BI GAGCTC 1 cut(s) 1905
Psp5II RGGWCCY 1 cut(s) 401
Psp6I CCWGG 5 cut(s) 161, 421, 540, 1359, 1749
PspFI CCCAGC 2 cut(s) 1792, 2118
PspGI CCWGG 5 cut(s) 161, 421, 540, 1359, 1749
PspN4I GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
PspPI GGNCC 8 cut(s) 147, 401, 440, 453, 544, 1047, 1063, 1172
PspPPI RGGWCCY 1 cut(s) 401
PstI CTGCAG 2 cut(s) 1419, 1786
PstNI CAGNNNCTG 1 cut(s) 1352
PsuI RGATCY 3 cut(s) 412, 1633, 2084
PsyI GACNNNGTC 1 cut(s) 827
PvuII CAGCTG 4 cut(s) 104, 557, 683, 1787
RsaI GTAC 1 cut(s) 1943
RsaNI GTAC 1 cut(s) 1942
RseI CAYNNNNRTG 2 cut(s) 1227, 1990
SacI GAGCTC 1 cut(s) 1905
SaqAI TTAA 5 cut(s) 251, 564, 1023, 1640, 1979
SatI GCNGC 9 cut(s) 123, 432, 706, 738, 1415, 1755, 1782, 1785, 2123
Sau3AI GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
Sau96I GGNCC 8 cut(s) 147, 401, 440, 453, 544, 1047, 1063, 1172
SchI GAGTC 5 cut(s) 92, 924, 1592, 2012, 2044
ScrFI CCNGG 6 cut(s) 163, 423, 542, 1321, 1361, 1751
SduI GDGCHC 3 cut(s) 242, 817, 1905
SfcI CTRYAG 2 cut(s) 1415, 1782
SinI GGWCC 3 cut(s) 401, 440, 1047
SmiMI CAYNNNNRTG 2 cut(s) 1227, 1990
SmlI CTYRAG 4 cut(s) 511, 1770, 1904, 2088
SmoI CTYRAG 4 cut(s) 511, 1770, 1904, 2088
SphI GCATGC 1 cut(s) 1262
Sse9I AATT 9 cut(s) 142, 354, 637, 697, 709, 747, 1024, 1192, 1680
SseBI AGGCCT 1 cut(s) 166
SsiI CCGC 6 cut(s) 705, 737, 979, 1450, 1754, 2122
SspMI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
SstI GAGCTC 1 cut(s) 1905
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 6 cut(s) 161, 421, 540, 1319, 1359, 1749
StyI CCWWGG 1 cut(s) 2139
TaaI ACNGT 7 cut(s) 478, 718, 835, 1131, 1159, 1855, 1877
TaiI ACGT 5 cut(s) 239, 339, 964, 1604, 1741
TaqI TCGA 8 cut(s) 242, 502, 1096, 1295, 1304, 1834, 2033, 2070
TasI AATT 9 cut(s) 142, 354, 637, 697, 709, 747, 1024, 1192, 1680
TauI GCSGC 4 cut(s) 708, 740, 1757, 2125
TfiI GAWTC 7 cut(s) 43, 77, 575, 805, 910, 1108, 1249
Tru1I TTAA 5 cut(s) 251, 564, 1023, 1640, 1979
Tru9I TTAA 5 cut(s) 251, 564, 1023, 1640, 1979
TscAI CASTG 7 cut(s) 39, 295, 326, 840, 853, 1168, 1260
TseFI GTSAC 3 cut(s) 829, 1229, 2015
TseI GCWGC 5 cut(s) 122, 431, 1414, 1781, 1784
Tsp45I GTSAC 3 cut(s) 829, 1229, 2015
TspDTI ATGAA 8 cut(s) 69, 275, 302, 588, 933, 1211, 1327, 1557
TspRI CASTG 7 cut(s) 39, 295, 326, 840, 853, 1168, 1260
Tth111I GACNNNGTC 1 cut(s) 827
VpaK11BI GGWCC 3 cut(s) 401, 440, 1047
XagI CCTNNNNNAGG 1 cut(s) 1070
XapI RAATTY 1 cut(s) 354
XceI RCATGY 1 cut(s) 1262
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 1 cut(s) 1330
XspI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
Zsp2I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.