MD07G1173100.v1.1

VWA / Hh protein intein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012886
Physical Location & Seq
Forward (+)
25074959 .. 25079289
4331 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1173100.v1.1.491

Sequence Viewer

Length: 2160 bp
ATGGGGAGCAAATGGAGGAAAGCAAAGCTTGCACTGGGGCTGAATCTGTGTTTATATCTTCCCAAAACACTAGAGGATTCATCTCCCTCCTTTGTGGACTCAGCTGAGAGGTTGTCTGATGCTGCCCTGCTTTCACCTGCCAATTGGGCCGATGGCTCCTCCAGGCCTACCACTCCAACTCCATCCTCCAATGGTTTGAAGCTCTCCAGAAATGGAAGCAAATCCTCAAAGCAAACGTGCTCGATATGCTTAACCAAAATGAAACAAGGAGGTGGCCATGCTCTTTTCACCGCTGAATGTTCACATTCTTTTCATTTTCACTGCATCACATCAAACGTGAAACATGGCAACCAAATTTGCCCAGTTTGTAGGGCAAAGTGGAAAGAAATCCCAGAGCAGGGTCCTAGTTTGGATCCTCCCCCTGGAAGGGCAGCTATTGGTCCGGTAGGTTGGCCCCAAAATGATGCTTTGATGACTGTGGTTCGCCGACTTCCTCCTTCTCGAAGAGACTTGAGCCGACCACACATCATGCCACTTTACCAGGCTCATGAGCCAGCTGTATTTAATGATGATGAATCCTTAGACCAACCTGTGTTTGCTGAGAGAAGCTCGTGCGATAAAAATGGTGCAGATGACAATTTGGTTAGGATGATAGAGATCAAAACATTCCCAGAGGTTTCAGCTGTTTCAAAATCCAATTCTTGCGGCAATTTTACAGTTTTGATCCATCTGAAAGCGGCTACTTCAATTACAAGACAAAATCCTAGCAGAAATCAGTCCACTTTGCCGCCTCAGTTTTCTCAGAATCATCGTGCTCCAGTAGACCTAGTCACAGTGCTTGACACCAGCGGTAGCATGGCAGGAACCAAGCTTGCATTGCTGAAAAGGGCTATGGGGTTCGTAATACAGAATCTTGGCTCCAATGACAGACTCTCGGTTATTGCATTCTCTTCAACAGCACGTCGCCTCTTTCCCCTCCGCCGGATGACTGACACTGGACGCCAGCAGGCATTGCAAGCTGTTAACTCTCTGGTCGCAAATGGTGGGACCAACATTGCCGAGGGCCTAAGGAAGGGTGGCAAGGTATTGGAAGATCGAAGGGGAAAGAATCCTGTTGCCAGTATAATACTGTTGTCAGATGGGCAGGACACTTATACCATCAGTGGCTCTGGGCCTAACCAATCTCAGCCAAATTATAAGTTGCTCCTCCCTTTGTCTATTCATGGCGGTGACAATACAGGCTTCCAGATTCCAGTGCATGCATTTGGATTTGGTGGAGATCATGATGCATCATCGATGCACTCAATTTCAGAGATATCTGGGGGAACATTTTCTTTCATTGAGACAGAAGCTGTCATCCAGGATGCATTTGCACAATGCATTGGAGGGCTTTTGAGTGTTGTGGTGCAAGAGCTGCAGGTGGCAGTTGAGTGTGTGAACACAAGTCTCCGCCTCGGTTCACTAAAAGCAGGAAGCTACCCAAGTCGTGTGATGGCTGATGGACTGAAAGGGGTAATTGATGTTGGAGGTATGTACGCTGATGAAGAGAGGGATTTTCTGGTTTCAGTAAATGTCCCAACAGAGTCTTGTAGCAATGTAACATCATTGATAAAGGTGAAATGCATTTACAAGGATCCCTTAAATAAAGAAATGGCGACAATAGAGAGTGAAGAAGTTAGAATCGAGAGACCTGATGTAGTTGGGCAAGCAGTAGTGTCAATAGAAGTGGACAGGCAACGTAATAGGCTCCAGGCGGCCGAGGCAATGGCTCAAGCACGAGCTGCAGCTGAGCTGGGAGACTTAGCTGGTGCAGTTTCAACCCTTGAAATTTGTCGAAGGGTATTGTCAGACACCGTGTCAGCAAAATCCCAAGACCATCTGTGTGTAGCATTGGATGCTGAGCTCAAGGAAATGCAAGAGAGGATGGCAAGCAGACATGTGTACGAGGCATCTGGCAGAGCATACATCCTTTCAGGATTAAGCTCACACTCATGGCAACGAGCGACAGCTAGAGGTGACTCTACTGATGGTTCGAGTCTTGTTCAGGCTTATCAAACCCCATCAATGGTCGAGATGCTTACTAGATCTCAAGCTACATTGTTGGGTAGTCCATCAGCCCAGCGGCTAGTCCGACCAACCAAAGCCGAGGTAATCATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA Vwaint zf-RING_11 zf-RING_2

Protein Analysis

720

Amino Acids

77.45

Weight (kDa)

8.27

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 77 - 124 3.3e-07 RING-H2 zinc finger domain
zf-RING_2 PF13639 79 - 124 1.3e-07 Ring finger domain
zf-RING_11 PF17123 80 - 109 2.8e-08 RING-like zinc finger
zf-C3HC4 PF00097 80 - 123 1.5e-07 Zinc finger, C3HC4 type (RING finger)
VWA_CoxE PF05762 259 - 388 2e-08 VWA domain containing CoxE-like protein
VWA PF00092 275 - 457 2.4e-22 von Willebrand factor type A domain
VWA_3 PF13768 275 - 446 8.9e-17 von Willebrand factor type A domain
VWA_2 PF13519 276 - 382 2.1e-20 von Willebrand factor type A domain
WAV3_C PF25243 576 - 646 8.3e-06 E3 ubiquitin-protein ligase WAV3-like, C-terminal domain
Vwaint PF14624 625 - 697 1.4e-20 VWA / Hh protein intein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012886)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G38970
fragaria_vesca FvH4_7g19190 FvH4_7g19190
malus_domestica MD01G1107300.v1.1 MD07G1173100.v1.1
pyrus_communis pycom01g13410 pycom07g16770
rosa_chinensis RchiOBHm_Chr1g0363051
rosa_laevigata RLG00000027598
rosa_multiflora Rmu_sc0004472.1_g000004
rosa_roxburghii Rroxscaffold_4G00293520
rosa_rugosa Rorug01G0304000 Rorug01G0304000
rosa_samantha Rh1AG313000 Rh1BG276200 Rh1CG293000 Rh1DG306900
rosa_wichuraiana Rw1G027730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1197
AarI CACCTGC 2 cut(s) 145, 1408
Acc36I ACCTGC 2 cut(s) 145, 1408
AccI GTMKAC 1 cut(s) 822
AclWI GGATC 5 cut(s) 407, 420, 718, 1628, 1641
AcoI YGGCCR 2 cut(s) 274, 1755
AcsI RAATTY 2 cut(s) 354, 1827
AcyI GRCGYC 1 cut(s) 1000
AfaI GTAC 2 cut(s) 1535, 1943
AfiI CCNNNNNNNGG 8 cut(s) 397, 398, 422, 426, 427, 981, 1072, 2065
AflIII ACRYGT 1 cut(s) 1936
AgsI TTSAA 6 cut(s) 199, 690, 747, 954, 1818, 1826
AhdI GACNNNNNGTC 1 cut(s) 1855
AjiI CACGTC 1 cut(s) 962
AjnI CCWGG 5 cut(s) 161, 421, 540, 1359, 1749
Alw21I GWGCWC 3 cut(s) 242, 817, 1905
Alw26I GTCTC 5 cut(s) 501, 1337, 1451, 1681, 1791
AlwI GGATC 5 cut(s) 407, 420, 718, 1628, 1641
AlwNI CAGNNNCTG 1 cut(s) 1352
AoxI GGCC 7 cut(s) 147, 164, 274, 452, 1063, 1172, 1755
ApeKI GCWGC 5 cut(s) 122, 431, 1414, 1781, 1784
ApoI RAATTY 2 cut(s) 354, 1827
Asp700I GAANNNNTTC 1 cut(s) 1330
AspS9I GGNCC 7 cut(s) 147, 401, 440, 453, 1047, 1063, 1172
AsuHPI GGTGA 5 cut(s) 126, 280, 1241, 1627, 2027
AvaII GGWCC 3 cut(s) 401, 440, 1047
AxyI CCTNAGG 1 cut(s) 1067
BalI TGGCCA 1 cut(s) 276
BamHI GGATCC 2 cut(s) 412, 1633
BanII GRGCYC 1 cut(s) 1905
BauI CACGAG 2 cut(s) 610, 1776
Bbv12I GWGCWC 3 cut(s) 242, 817, 1905
BbvI GCAGC 5 cut(s) 109, 443, 1401, 1768, 1796
BciT130I CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
BcoDI GTCTC 5 cut(s) 501, 1337, 1451, 1681, 1791
BfaI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
BfmI CTRYAG 2 cut(s) 1415, 1782
BfuAI ACCTGC 2 cut(s) 145, 1408
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 1 cut(s) 2084
BlpI GCTNAGC 2 cut(s) 1788, 1899
Bme1390I CCNGG 5 cut(s) 163, 423, 542, 1361, 1751
Bme18I GGWCC 3 cut(s) 401, 440, 1047
BmeRI GACNNNNNGTC 1 cut(s) 1855
BmgBI CACGTC 1 cut(s) 962
BmgT120I GGNCC 7 cut(s) 147, 401, 440, 453, 1047, 1063, 1172
BmiI GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
BmrFI CCNGG 5 cut(s) 163, 423, 542, 1361, 1751
BmrI ACTGGG 2 cut(s) 44, 356
BmuI ACTGGG 2 cut(s) 44, 356
BplI GAGNNNNNCTC 2 cut(s) 593, 625
BpmI CTGGAG 4 cut(s) 145, 190, 801, 1733
Bpu1102I GCTNAGC 2 cut(s) 1788, 1899
BpuEI CTTGAG 4 cut(s) 532, 1755, 1889, 2073
Bsa29I ATCGAT 1 cut(s) 1295
BsaBI GATNNNNATC 1 cut(s) 656
BsaHI GRCGYC 1 cut(s) 1000
BsaI GGTCTC 1 cut(s) 1681
BsaJI CCNNGG 5 cut(s) 421, 1059, 1453, 1758, 2145
BsaWI WCCGGW 1 cut(s) 442
BsaXI ACNNNNNCTCC 2 cut(s) 163, 193
Bsc4I CCNNNNNNNGG 8 cut(s) 397, 398, 422, 426, 427, 981, 1072, 2065
Bse1I ACTGG 6 cut(s) 39, 362, 818, 1000, 1119, 1253
Bse21I CCTNAGG 1 cut(s) 1067
Bse3DI GCAATG 5 cut(s) 875, 1010, 1053, 1600, 1770
Bse8I GATNNNNATC 1 cut(s) 656
BseBI CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
BseCI ATCGAT 1 cut(s) 1295
BseDI CCNNGG 5 cut(s) 421, 1059, 1453, 1758, 2145
BseGI GGATG 8 cut(s) 182, 654, 990, 1356, 1369, 1900, 1929, 1965
BseJI GATNNNNATC 1 cut(s) 656
BseLI CCNNNNNNNGG 8 cut(s) 397, 398, 422, 426, 427, 981, 1072, 2065
BseMI GCAATG 5 cut(s) 875, 1010, 1053, 1600, 1770
BseMII CTCAG 8 cut(s) 96, 114, 591, 806, 815, 1199, 1779, 1890
BseNI ACTGG 6 cut(s) 39, 362, 818, 1000, 1119, 1253
BseRI GAGGAG 2 cut(s) 148, 1196
BseX3I CGGCCG 1 cut(s) 1755
BseXI GCAGC 5 cut(s) 109, 443, 1401, 1768, 1796
BseYI CCCAGC 2 cut(s) 1792, 2118
BsgI GTGCAG 2 cut(s) 648, 1830
Bsh1285I CGRYCG 1 cut(s) 1758
BshFI GGCC 7 cut(s) 149, 166, 276, 454, 1065, 1174, 1757
BshVI ATCGAT 1 cut(s) 1295
BsiEI CGRYCG 1 cut(s) 1758
BsiHKAI GWGCWC 3 cut(s) 242, 817, 1905
BsiSI CCGG 2 cut(s) 443, 982
BslFI GGGAC 2 cut(s) 1060, 1559
BslI CCNNNNNNNGG 8 cut(s) 397, 398, 422, 426, 427, 981, 1072, 2065
BsmAI GTCTC 5 cut(s) 501, 1337, 1451, 1681, 1791
BsmFI GGGAC 2 cut(s) 1060, 1559
BsmI GAATGC 1 cut(s) 944
BsnI GGCC 7 cut(s) 149, 166, 276, 454, 1065, 1174, 1757
Bso31I GGTCTC 1 cut(s) 1681
Bsp1286I GDGCHC 3 cut(s) 242, 817, 1905
Bsp143I GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
Bsp1720I GCTNAGC 2 cut(s) 1788, 1899
BspANI GGCC 7 cut(s) 149, 166, 276, 454, 1065, 1174, 1757
BspCNI CTCAG 8 cut(s) 97, 113, 592, 805, 814, 1198, 1780, 1891
BspDI ATCGAT 1 cut(s) 1295
BspHI TCATGA 2 cut(s) 547, 1282
BspLI GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
BspMAI CTGCAG 2 cut(s) 1419, 1786
BspMI ACCTGC 2 cut(s) 145, 1408
BspPI GGATC 5 cut(s) 407, 420, 718, 1628, 1641
BspTNI GGTCTC 1 cut(s) 1681
BsrDI GCAATG 5 cut(s) 875, 1010, 1053, 1600, 1770
BsrI ACTGG 6 cut(s) 39, 362, 818, 1000, 1119, 1253
BssECI CCNNGG 5 cut(s) 421, 1059, 1453, 1758, 2145
BssMI GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
BssNI GRCGYC 1 cut(s) 1000
BssSI CACGAG 2 cut(s) 610, 1776
Bst2BI CACGAG 2 cut(s) 610, 1776
Bst2UI CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
Bst4CI ACNGT 5 cut(s) 478, 718, 835, 1131, 1855
Bst6I CTCTTC 3 cut(s) 499, 955, 1539
BstACI GRCGYC 1 cut(s) 1000
BstAPI GCANNNNNTGC 5 cut(s) 29, 1012, 1414, 1781, 1895
BstC8I GCNNGC 9 cut(s) 30, 555, 873, 1004, 1008, 1017, 1260, 1707, 1930
BstENI CCTNNNNNAGG 1 cut(s) 1070
BstF5I GGATG 8 cut(s) 182, 654, 990, 1356, 1369, 1900, 1929, 1965
BstKTI GATC 7 cut(s) 415, 660, 726, 1096, 1282, 1636, 2087
BstMAI GTCTC 5 cut(s) 501, 1337, 1451, 1681, 1791
BstMBI GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
BstMCI CGRYCG 1 cut(s) 1758
BstNI CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
BstNSI RCATGY 2 cut(s) 1262, 1940
BstSCI CCNGG 5 cut(s) 161, 421, 540, 1359, 1749
BstSFI CTRYAG 2 cut(s) 1415, 1782
BstV1I GCAGC 5 cut(s) 109, 443, 1401, 1768, 1796
BstX2I RGATCY 3 cut(s) 412, 1633, 2084
BstYI RGATCY 3 cut(s) 412, 1633, 2084
BstZI CGGCCG 1 cut(s) 1755
Bsu15I ATCGAT 1 cut(s) 1295
Bsu36I CCTNAGG 1 cut(s) 1067
BsuRI GGCC 7 cut(s) 149, 166, 276, 454, 1065, 1174, 1757
BsuTUI ATCGAT 1 cut(s) 1295
BtrI CACGTC 1 cut(s) 962
BtsCI GGATG 8 cut(s) 182, 654, 990, 1356, 1369, 1900, 1929, 1965
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 6 cut(s) 32, 319, 840, 993, 1168, 1260
BveI ACCTGC 2 cut(s) 145, 1408
Cac8I GCNNGC 9 cut(s) 30, 555, 873, 1004, 1008, 1017, 1260, 1707, 1930
CaiI CAGNNNCTG 1 cut(s) 1352
CciI TCATGA 2 cut(s) 547, 1282
Cfr13I GGNCC 7 cut(s) 147, 401, 440, 453, 1047, 1063, 1172
ClaI ATCGAT 1 cut(s) 1295
CseI GACGC 1 cut(s) 1008
Csp6I GTAC 2 cut(s) 1534, 1942
CspCI CAANNNNNGTGG 2 cut(s) 1707, 1742
CviQI GTAC 2 cut(s) 1534, 1942
DpnI GATC 7 cut(s) 414, 659, 725, 1095, 1281, 1635, 2086
DpnII GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
DriI GACNNNNNGTC 1 cut(s) 1855
EaeI YGGCCR 2 cut(s) 274, 1755
EagI CGGCCG 1 cut(s) 1755
Eam1104I CTCTTC 3 cut(s) 499, 955, 1539
Eam1105I GACNNNNNGTC 1 cut(s) 1855
EarI CTCTTC 3 cut(s) 499, 955, 1539
EciI GGCGGA 2 cut(s) 968, 1439
Ecl136II GAGCTC 1 cut(s) 1903
EclXI CGGCCG 1 cut(s) 1755
Eco147I AGGCCT 1 cut(s) 166
Eco24I GRGCYC 1 cut(s) 1905
Eco31I GGTCTC 1 cut(s) 1681
Eco32I GATATC 1 cut(s) 1317
Eco47I GGWCC 3 cut(s) 401, 440, 1047
Eco52I CGGCCG 1 cut(s) 1755
Eco53kI GAGCTC 1 cut(s) 1903
Eco81I CCTNAGG 1 cut(s) 1067
EcoICRI GAGCTC 1 cut(s) 1903
EcoNI CCTNNNNNAGG 1 cut(s) 1070
EcoO109I RGGNCCY 2 cut(s) 401, 1063
EcoRII CCWGG 5 cut(s) 161, 421, 540, 1359, 1749
EcoRV GATATC 1 cut(s) 1317
EcoT22I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
EcoT38I GRGCYC 1 cut(s) 1905
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 1622, 1654
FaqI GGGAC 2 cut(s) 1060, 1559
FblI GTMKAC 1 cut(s) 822
FokI GGATG 8 cut(s) 169, 661, 997, 1343, 1376, 1907, 1936, 1952
FriOI GRGCYC 1 cut(s) 1905
FspBI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
GsaI CCCAGC 2 cut(s) 1796, 2122
GsuI CTGGAG 4 cut(s) 145, 190, 801, 1733
HaeIII GGCC 7 cut(s) 149, 166, 276, 454, 1065, 1174, 1757
HapII CCGG 2 cut(s) 443, 982
HgaI GACGC 1 cut(s) 1008
Hin1I GRCGYC 1 cut(s) 1000
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 2 cut(s) 26, 869
HpaI GTTAAC 1 cut(s) 1024
HpaII CCGG 2 cut(s) 443, 982
HphI GGTGA 5 cut(s) 126, 280, 1241, 1627, 2027
Hpy166II GTNNAC 9 cut(s) 97, 302, 780, 823, 1024, 1438, 1460, 1729, 1942
Hpy188I TCNGA 7 cut(s) 118, 732, 804, 1138, 1312, 1849, 2132
Hpy188III TCNNGA 8 cut(s) 207, 501, 548, 1246, 1283, 1684, 1974, 2071
Hpy8I GTNNAC 9 cut(s) 97, 302, 780, 823, 1024, 1438, 1460, 1729, 1942
Hpy99I CGWCG 1 cut(s) 966
HpyAV CCTTC 5 cut(s) 420, 507, 1066, 1092, 1830
HpyCH4III ACNGT 5 cut(s) 478, 718, 835, 1131, 1855
HpyCH4IV ACGT 4 cut(s) 236, 336, 961, 1738
HpySE526I ACGT 4 cut(s) 236, 336, 961, 1738
Hsp92I GRCGYC 1 cut(s) 1000
KspAI GTTAAC 1 cut(s) 1024
Kzo9I GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
LmnI GCTCC 6 cut(s) 6, 161, 820, 923, 1209, 1752
Lsp1109I GCAGC 5 cut(s) 109, 443, 1401, 1768, 1796
MaeI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
MaeII ACGT 4 cut(s) 236, 336, 961, 1738
MaeIII GTNAC 4 cut(s) 829, 1229, 1597, 2015
MalI GATC 7 cut(s) 414, 659, 725, 1095, 1281, 1635, 2086
MboI GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
MboII GAAGA 6 cut(s) 50, 516, 942, 1103, 1556, 1682
MfeI CAATTG 1 cut(s) 142
MflI RGATCY 3 cut(s) 412, 1633, 2084
MhlI GDGCHC 3 cut(s) 242, 817, 1905
MlsI TGGCCA 1 cut(s) 276
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 5 cut(s) 92, 924, 1592, 2012, 2044
MmeI TCCRAC 3 cut(s) 200, 1504, 2155
Mox20I TGGCCA 1 cut(s) 276
Mph1103I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
MroXI GAANNNNTTC 1 cut(s) 1330
MscI TGGCCA 1 cut(s) 276
MseI TTAA 5 cut(s) 251, 564, 1023, 1640, 1979
MslI CAYNNNNRTG 3 cut(s) 1227, 1881, 1990
Msp20I TGGCCA 1 cut(s) 276
MspA1I CMGCKG 7 cut(s) 104, 293, 557, 683, 849, 1787, 2122
MspI CCGG 2 cut(s) 443, 982
MspR9I CCNGG 5 cut(s) 163, 423, 542, 1361, 1751
MunI CAATTG 1 cut(s) 142
Mva1269I GAATGC 1 cut(s) 944
MvaI CCWGG 5 cut(s) 163, 423, 542, 1361, 1751
NdeII GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
NlaIV GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
NmeAIII GCCGAG 2 cut(s) 1084, 1783
NmuCI GTSAC 3 cut(s) 829, 1229, 2015
NsiI ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
NspI RCATGY 2 cut(s) 1262, 1940
PaeI GCATGC 1 cut(s) 1262
PagI TCATGA 2 cut(s) 547, 1282
PaqCI CACCTGC 2 cut(s) 145, 1408
PceI AGGCCT 1 cut(s) 166
PciI ACATGT 1 cut(s) 1936
PctI GAATGC 1 cut(s) 944
PdmI GAANNNNTTC 1 cut(s) 1330
PfeI GAWTC 8 cut(s) 43, 77, 575, 805, 910, 1108, 1249, 1680
PflFI GACNNNGTC 1 cut(s) 827
PfoI TCCNGGA 1 cut(s) 1359
PleI GAGTC 5 cut(s) 92, 924, 1591, 2012, 2043
PpsI GAGTC 5 cut(s) 92, 924, 1591, 2012, 2043
PpuMI RGGWCCY 1 cut(s) 401
PscI ACATGT 1 cut(s) 1936
PsiI TTATAA 1 cut(s) 1197
Psp124BI GAGCTC 1 cut(s) 1905
Psp5II RGGWCCY 1 cut(s) 401
Psp6I CCWGG 5 cut(s) 161, 421, 540, 1359, 1749
PspFI CCCAGC 2 cut(s) 1792, 2118
PspGI CCWGG 5 cut(s) 161, 421, 540, 1359, 1749
PspN4I GGNNCC 9 cut(s) 157, 402, 414, 455, 865, 919, 1048, 1635, 1748
PspPI GGNCC 7 cut(s) 147, 401, 440, 453, 1047, 1063, 1172
PspPPI RGGWCCY 1 cut(s) 401
PstI CTGCAG 2 cut(s) 1419, 1786
PstNI CAGNNNCTG 1 cut(s) 1352
PsuI RGATCY 3 cut(s) 412, 1633, 2084
PsyI GACNNNGTC 1 cut(s) 827
PvuII CAGCTG 4 cut(s) 104, 557, 683, 1787
RsaI GTAC 2 cut(s) 1535, 1943
RsaNI GTAC 2 cut(s) 1534, 1942
RseI CAYNNNNRTG 3 cut(s) 1227, 1881, 1990
SacI GAGCTC 1 cut(s) 1905
SaqAI TTAA 5 cut(s) 251, 564, 1023, 1640, 1979
Sau3AI GATC 7 cut(s) 412, 657, 723, 1093, 1279, 1633, 2084
Sau96I GGNCC 7 cut(s) 147, 401, 440, 453, 1047, 1063, 1172
SchI GAGTC 5 cut(s) 92, 924, 1592, 2012, 2044
ScrFI CCNGG 5 cut(s) 163, 423, 542, 1361, 1751
SduI GDGCHC 3 cut(s) 242, 817, 1905
SfcI CTRYAG 2 cut(s) 1415, 1782
SinI GGWCC 3 cut(s) 401, 440, 1047
SmiMI CAYNNNNRTG 3 cut(s) 1227, 1881, 1990
SmlI CTYRAG 4 cut(s) 511, 1770, 1904, 2088
SmoI CTYRAG 4 cut(s) 511, 1770, 1904, 2088
SphI GCATGC 1 cut(s) 1262
SseBI AGGCCT 1 cut(s) 166
SspMI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
SstI GAGCTC 1 cut(s) 1905
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 5 cut(s) 161, 421, 540, 1359, 1749
TaaI ACNGT 5 cut(s) 478, 718, 835, 1131, 1855
TaiI ACGT 4 cut(s) 239, 339, 964, 1741
TaqI TCGA 8 cut(s) 242, 502, 1096, 1295, 1683, 1834, 2033, 2070
TauI GCSGC 5 cut(s) 708, 740, 790, 1757, 2125
TfiI GAWTC 8 cut(s) 43, 77, 575, 805, 910, 1108, 1249, 1680
Tru1I TTAA 5 cut(s) 251, 564, 1023, 1640, 1979
Tru9I TTAA 5 cut(s) 251, 564, 1023, 1640, 1979
TscAI CASTG 6 cut(s) 39, 326, 840, 1000, 1168, 1260
TseFI GTSAC 3 cut(s) 829, 1229, 2015
TseI GCWGC 5 cut(s) 122, 431, 1414, 1781, 1784
Tsp45I GTSAC 3 cut(s) 829, 1229, 2015
TspDTI ATGAA 7 cut(s) 69, 275, 302, 588, 1211, 1327, 1557
TspRI CASTG 6 cut(s) 39, 326, 840, 1000, 1168, 1260
Tth111I GACNNNGTC 1 cut(s) 827
VpaK11BI GGWCC 3 cut(s) 401, 440, 1047
XagI CCTNNNNNAGG 1 cut(s) 1070
XapI RAATTY 2 cut(s) 354, 1827
XceI RCATGY 2 cut(s) 1262, 1940
XcmI CCANNNNNNNNNTGG 1 cut(s) 853
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 1 cut(s) 1330
XspI CTAG 7 cut(s) 71, 405, 765, 827, 2010, 2082, 2126
Zsp2I ATGCAT 5 cut(s) 1264, 1291, 1369, 1382, 1625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.