MD01G1121300.v1.1

psbP-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0009485
Physical Location & Seq
Reverse (-)
23496971 .. 23499294
2324 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1121300.v1.1.491

Sequence Viewer

Length: 891 bp
ATGCCTAAGCAATGCAAGGTGTGTCCTATGACAAGTATAACGGATAAGTCGTCCAAAAAATGGAAGGAAACAGATAAGGTGATCGCCACAAGACTCCACACACGCGTACACCGAGTCCATATTTCTTCCTGCTTCACTCACAATTCAACAAAACCACCAACAATGGCGACCCTGCAGAACTCACCCTCCCTCCACAGGACCCCTGTCCTTAACTCCTTCACCAAGCTACACAGGCAAAGCACATTCCATTGCTGTAGAAAAAGTAATTCTTTTCTGGTAAGAGCAGATCAGGCTTCAGCAACTTCTACATGCTCTCCTTCTCAAGATAGATCCGGGAGACGCCAAGTGCTAGCAGCAGGAACAGTTGCTTCTTGGGTTCTTCTAACCAACCAACATTCTGTATCATTTGCTGCAGAAAATAAAAAGGGATTTATTCCTGTCACCGACAAGAAAGATGGATACGAATTTGTCTACCCTTTTGGATGGCAGGAAGTAACCATTGAAGGTCAAGACAAGGTATTCAAGGATGTCATTGAGCCGTTAGAGACTGCAAGTGTGACTCTGTTCCCAACCGTCAAGCAAGACATCAAAGAATTCGGGTCCCCGCAACAGGTGGCTGAAACTTTGATCAAGAAGGTTCTGTCTCCTCCTACCCAGAAAACAAAGCTGATTGAGGCAGTAGAGCACGACATCGATGGGAAAACATATTACACATTTGAGTTCCTTGCTAAGGCTCCAAACTATACTCGCCACGCTCTCAGCACAGTAGCTATCGGCAATGGCAACTTCTACACCTTGACGACGGGGGCTAACGAGAGGAGGTGGGAAAAGATGAAAGACAAGTTACACACCATCGTCGATTCCTTCAAGCTTTTCAAAGTCTTCGATTAA

Protein Analysis

297

Amino Acids

33.43

Weight (kDa)

9.5

Isoelectric Point (pI)

41.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 142 - 290 7.4e-54 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 60
AccI GTMKAC 1 cut(s) 471
AccII CGCG 1 cut(s) 105
AciI CCGC 1 cut(s) 605
AclWI GGATC 1 cut(s) 324
AcsI RAATTY 2 cut(s) 464, 593
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 340
AfaI GTAC 1 cut(s) 108
AfiI CCNNNNNNNGG 4 cut(s) 60, 195, 610, 730
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 5 cut(s) 147, 503, 523, 868, 877
AloI GAACNNNNNNTCC 2 cut(s) 170, 202
AluBI AGCT 4 cut(s) 226, 667, 770, 871
AluI AGCT 4 cut(s) 226, 667, 770, 871
Alw21I GWGCWC 1 cut(s) 687
Alw26I GTCTC 3 cut(s) 331, 539, 648
AlwI GGATC 1 cut(s) 324
ApeKI GCWGC 2 cut(s) 353, 410
ApoI RAATTY 2 cut(s) 464, 593
AspS9I GGNCC 2 cut(s) 198, 600
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 4 cut(s) 91, 174, 211, 433
AsuNHI GCTAGC 1 cut(s) 349
AvaII GGWCC 2 cut(s) 198, 600
BbsI GAAGAC 1 cut(s) 874
Bbv12I GWGCWC 1 cut(s) 687
BbvI GCAGC 2 cut(s) 365, 397
BccI CCATC 4 cut(s) 449, 477, 689, 860
BceAI ACGGC 1 cut(s) 523
BciVI GTATCC 1 cut(s) 452
BclI TGATCA 1 cut(s) 627
BcnI CCSGG 1 cut(s) 334
BcoDI GTCTC 3 cut(s) 331, 539, 648
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 3 cut(s) 173, 253, 411
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 2 cut(s) 354, 411
BlsI GCNGC 2 cut(s) 355, 412
Bme1390I CCNGG 1 cut(s) 334
Bme18I GGWCC 2 cut(s) 198, 600
BmgT120I GGNCC 2 cut(s) 198, 600
BmiI GGNNCC 4 cut(s) 200, 601, 602, 735
BmrFI CCNGG 1 cut(s) 334
BmtI GCTAGC 1 cut(s) 353
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 874
Bpu10I CCTNAGC 2 cut(s) 6, 729
BpuEI CTTGAG 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 334
Bsa29I ATCGAT 1 cut(s) 693
BsaHI GRCGYC 1 cut(s) 340
BsaXI ACNNNNNCTCC 6 cut(s) 170, 174, 200, 204, 298, 328
Bsc4I CCNNNNNNNGG 4 cut(s) 60, 195, 610, 730
Bse3DI GCAATG 3 cut(s) 17, 247, 784
BseCI ATCGAT 1 cut(s) 693
BseGI GGATG 2 cut(s) 488, 532
BseLI CCNNNNNNNGG 4 cut(s) 60, 195, 610, 730
BseMI GCAATG 3 cut(s) 17, 247, 784
BseMII CTCAG 1 cut(s) 772
BseRI GAGGAG 2 cut(s) 636, 832
BseXI GCAGC 2 cut(s) 365, 397
Bsh1236I CGCG 1 cut(s) 105
BshVI ATCGAT 1 cut(s) 693
BsiHKAI GWGCWC 1 cut(s) 687
BsiSI CCGG 1 cut(s) 333
BslFI GGGAC 1 cut(s) 586
BslI CCNNNNNNNGG 4 cut(s) 60, 195, 610, 730
BsmAI GTCTC 3 cut(s) 331, 539, 648
BsmBI CGTCTC 1 cut(s) 331
BsmFI GGGAC 1 cut(s) 586
Bsp1286I GDGCHC 1 cut(s) 687
Bsp143I GATC 4 cut(s) 81, 286, 329, 627
BspACI CCGC 1 cut(s) 605
BspCNI CTCAG 1 cut(s) 771
BspDI ATCGAT 1 cut(s) 693
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 4 cut(s) 200, 601, 602, 735
BspMAI CTGCAG 2 cut(s) 177, 415
BspOI GCTAGC 1 cut(s) 353
BspPI GGATC 1 cut(s) 324
BsrDI GCAATG 3 cut(s) 17, 247, 784
BssMI GATC 4 cut(s) 81, 286, 329, 627
BssNI GRCGYC 1 cut(s) 340
Bst4CI ACNGT 3 cut(s) 364, 574, 766
BstACI GRCGYC 1 cut(s) 340
BstC8I GCNNGC 1 cut(s) 351
BstDEI CTNAG 3 cut(s) 6, 729, 758
BstENI CCTNNNNNAGG 1 cut(s) 728
BstF5I GGATG 2 cut(s) 488, 532
BstFNI CGCG 1 cut(s) 105
BstKTI GATC 4 cut(s) 84, 289, 332, 630
BstMAI GTCTC 3 cut(s) 331, 539, 648
BstMBI GATC 4 cut(s) 81, 286, 329, 627
BstMWI GCNNNNNNNGC 2 cut(s) 232, 290
BstNSI RCATGY 1 cut(s) 312
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 332
BstSFI CTRYAG 3 cut(s) 173, 253, 411
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 2 cut(s) 365, 397
BstV2I GAAGAC 1 cut(s) 874
BstX2I RGATCY 1 cut(s) 329
BstYI RGATCY 1 cut(s) 329
Bsu15I ATCGAT 1 cut(s) 693
BsuI GTATCC 1 cut(s) 452
BsuTUI ATCGAT 1 cut(s) 693
BtsCI GGATG 2 cut(s) 488, 532
Cac8I GCNNGC 1 cut(s) 351
Cfr13I GGNCC 2 cut(s) 198, 600
ClaI ATCGAT 1 cut(s) 693
CseI GACGC 1 cut(s) 348
Csp6I GTAC 1 cut(s) 107
CviAII CATG 1 cut(s) 309
CviJI RGCY 9 cut(s) 226, 293, 538, 617, 667, 734, 770, 809, 871
CviKI_1 RGCY 9 cut(s) 226, 293, 538, 617, 667, 734, 770, 809, 871
CviQI GTAC 1 cut(s) 107
DdeI CTNAG 3 cut(s) 6, 729, 758
DpnI GATC 4 cut(s) 83, 288, 331, 629
DpnII GATC 4 cut(s) 81, 286, 329, 627
Eco47I GGWCC 2 cut(s) 198, 600
Eco57I CTGAAG 1 cut(s) 279
EcoNI CCTNNNNNAGG 1 cut(s) 728
EcoO109I RGGNCCY 2 cut(s) 198, 600
EcoRI GAATTC 1 cut(s) 593
Esp3I CGTCTC 1 cut(s) 331
FaeI CATG 1 cut(s) 312
FaiI YATR 6 cut(s) 29, 38, 120, 310, 706, 744
FalI AAGNNNNNCTT 2 cut(s) 253, 285
FaqI GGGAC 1 cut(s) 586
FatI CATG 1 cut(s) 308
FauI CCCGC 1 cut(s) 612
FbaI TGATCA 1 cut(s) 627
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 2 cut(s) 354, 411
FokI GGATG 2 cut(s) 495, 539
Fsp4HI GCNGC 2 cut(s) 354, 411
FspBI CTAG 1 cut(s) 350
GluI GCNGC 2 cut(s) 354, 411
HapII CCGG 1 cut(s) 333
HgaI GACGC 1 cut(s) 348
Hin1I GRCGYC 1 cut(s) 340
Hin1II CATG 1 cut(s) 312
HindIII AAGCTT 1 cut(s) 869
HinfI GANTC 4 cut(s) 93, 114, 559, 860
HpaII CCGG 1 cut(s) 333
HphI GGTGA 4 cut(s) 91, 174, 211, 433
Hpy166II GTNNAC 2 cut(s) 109, 472
Hpy188III TCNNGA 3 cut(s) 323, 509, 631
Hpy8I GTNNAC 2 cut(s) 109, 472
Hpy99I CGWCG 2 cut(s) 805, 860
HpyAV CCTTC 6 cut(s) 58, 226, 327, 497, 628, 874
HpyCH4III ACNGT 3 cut(s) 364, 574, 766
HpyCH4V TGCA 4 cut(s) 15, 175, 413, 551
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 290
HpyF3I CTNAG 3 cut(s) 6, 729, 758
Hsp92I GRCGYC 1 cut(s) 340
Hsp92II CATG 1 cut(s) 312
KflI GGGWCCC 1 cut(s) 600
Ksp22I TGATCA 1 cut(s) 627
Kzo9I GATC 4 cut(s) 81, 286, 329, 627
LmnI GCTCC 1 cut(s) 739
Lsp1109I GCAGC 2 cut(s) 365, 397
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 4 cut(s) 439, 493, 556, 843
MalI GATC 4 cut(s) 83, 288, 331, 629
MboI GATC 4 cut(s) 81, 286, 329, 627
MboII GAAGA 3 cut(s) 117, 371, 874
MflI RGATCY 1 cut(s) 329
MhlI GDGCHC 1 cut(s) 687
MluCI AATT 4 cut(s) 142, 265, 464, 593
MluI ACGCGT 1 cut(s) 103
MlyI GAGTC 3 cut(s) 87, 123, 553
MnlI CCTC 6 cut(s) 196, 200, 657, 667, 810, 813
MseI TTAA 2 cut(s) 210, 889
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 334
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 232, 290
NciI CCSGG 1 cut(s) 334
NdeII GATC 4 cut(s) 81, 286, 329, 627
NheI GCTAGC 1 cut(s) 349
NlaIII CATG 1 cut(s) 312
NlaIV GGNNCC 4 cut(s) 200, 601, 602, 735
NmuCI GTSAC 2 cut(s) 439, 556
NspI RCATGY 1 cut(s) 312
PcsI WCGNNNNNNNCGW 2 cut(s) 47, 109
PfeI GAWTC 1 cut(s) 860
PflMI CCANNNNNTGG 1 cut(s) 60
PfoI TCCNGGA 1 cut(s) 332
PkrI GCNGC 2 cut(s) 355, 412
PleI GAGTC 3 cut(s) 87, 122, 553
PpsI GAGTC 3 cut(s) 87, 122, 553
PpuMI RGGWCCY 2 cut(s) 198, 600
PshAI GACNNNNGTC 1 cut(s) 203
Psp5II RGGWCCY 2 cut(s) 198, 600
PspN4I GGNNCC 4 cut(s) 200, 601, 602, 735
PspPI GGNCC 2 cut(s) 198, 600
PspPPI RGGWCCY 2 cut(s) 198, 600
PstI CTGCAG 2 cut(s) 177, 415
PsuI RGATCY 1 cut(s) 329
RsaI GTAC 1 cut(s) 108
RsaNI GTAC 1 cut(s) 107
SaqAI TTAA 2 cut(s) 210, 889
SatI GCNGC 2 cut(s) 354, 411
Sau3AI GATC 4 cut(s) 81, 286, 329, 627
Sau96I GGNCC 2 cut(s) 198, 600
SchI GAGTC 3 cut(s) 87, 123, 553
ScrFI CCNGG 1 cut(s) 334
SduI GDGCHC 1 cut(s) 687
SfcI CTRYAG 3 cut(s) 173, 253, 411
SinI GGWCC 2 cut(s) 198, 600
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
Sse9I AATT 4 cut(s) 142, 265, 464, 593
SsiI CCGC 1 cut(s) 605
SspMI CTAG 1 cut(s) 350
StyD4I CCNGG 1 cut(s) 332
TaaI ACNGT 3 cut(s) 364, 574, 766
TaqI TCGA 3 cut(s) 693, 858, 885
TasI AATT 4 cut(s) 142, 265, 464, 593
TfiI GAWTC 1 cut(s) 860
Tru1I TTAA 2 cut(s) 210, 889
Tru9I TTAA 2 cut(s) 210, 889
TseFI GTSAC 2 cut(s) 439, 556
TseI GCWGC 2 cut(s) 353, 410
Tsp45I GTSAC 2 cut(s) 439, 556
TspDTI ATGAA 1 cut(s) 848
TspGWI ACGGA 1 cut(s) 56
Van91I CCANNNNNTGG 1 cut(s) 60
VpaK11BI GGWCC 2 cut(s) 198, 600
XagI CCTNNNNNAGG 1 cut(s) 728
XapI RAATTY 2 cut(s) 464, 593
XceI RCATGY 1 cut(s) 312
XmiI GTMKAC 1 cut(s) 471
XspI CTAG 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.