Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011819

Physical Location & Seq

Genomic Coordinates Reverse (-)

24905696 .. 24910394

Gene Structure 4699 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1139400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1986 bp

ATGACAATTTTGGGCAATGGCGATTGGCTGCGAATCCCGTTGATCCTGAAAGTTTGCGGAATTGTGATGATGGCAGTCACCTTCTTCTACTTCGGGAAGCACTGGTCCGATGGGTCCCAGCAGCTACTCTTCTTCACCACCACCACGAGCCGAGCACCCTCCGCCGTCTCCGTCTCGCCCAACCTAGACAAGCCTTTCAATCTCTCCGCCCTCATAAACGAAACCCTAGCTCCACCCCCGTTGCTTCCGTCTCCGCCGCCGCCCGATGTGGTCCAGAGGTTCGGGATCGTCGACGAGAATGGCACCATGTCTGACGATTTCGAGGTCGGGGATTTCGATCCCGATGAGGTGGTGGAGAATTGGGGCGGCCAGAACGGGACTGTGGCGGAGAGTGAGGGGAGCGGGTCGGGTCGGGTGAGTGTGGAGAGGTTCGAGCTCTGCCCGAAGAGTATGAGCGAGTACATACCTTGTTTGGATAATGTGGAGGTGGTTAAGAGGCTGAAATCCACGGCGAAAGGCGAGCGGTTTGAGCGGCATTGCCCGGAGGCTGGTCGGGGTTTGAATTGCTTGGTTCCGGCGCCCAAGAGCTACAAGAGCCCAATTCCATGGCCTAAAAGCCGGGATGAGGTTTGGTTCTTCAACGTACCTCATACAAAATTAGTGGAAGATAAAGGGGGTCAGAATTGGATTATCAGACAGAAGAACAAGTTTAAGTTTCCGGGAGGTGGTACACAGTTTATACATGGAGCAGATGGTTACTTGACTGATATTTCTAAGGCGGTCCCAGACATCACCTTCGGTCAGCATGTCCGAGTTGTTTTGGATGTCGGATGTGGTGTTGCGAGTTTTGGTGCGTATCTGCTGTCGCGAGATGTCCTTACTATGTCAGTTGCACCTAAAGACGTCCATGAGAACCAGATTCAATTTGCTCTTGAGCGCGGTGTGCCTGCAATGGTAGCAGCATTTGCAACTCACCGTTTATTGTACCCAAGTCAGGCATTTGACCTGATACATTGCTCACGATGTAGGATTAACTGGACTCGTGATGATGGCATATTGTTGCTTGAGGTTAATAGGATGCTCAGGGCAGGAGGATACTTTGCTTGGGCAGCACAGCCAGTTTATAAGCATGAAGAAGTTCTAGAGGAGCAGTGGCAAGAGATGCTTAACCTCACCACGCGGCTTTGCTGGGAATTGGTGTCAAAGCAGGGCTATGTTGCAATATGGAAAAAGCCGGTTAACAACAGCTGCTATCTAAATCGGGATGAAGGAACCAAACCTCCATTATGTGATCCAAGTGATGACCCAGACAAAGTTTGGTATGTTGATCTGAAGGCATGCATCACAAGACTTCCTGAAAATGGCTATGGAACAAATGTCACAGCATGGCCGGACCGTTTGCAAACTCCCCCTGATAGGCTTCAGAGCATACAAATTGATGCCTTTATATCCAGAAAGGAGCTATTTAAAGCAGAATCCAGATATTGCCATGAGTTAGTTGGAGGTTATGTTCGTGGTTTAAAATGGAAGAAGTTTAGATTAAGAAACGTAATGGACATGAGAGCTGGCTTTGGAGGATTTGCAGCTGCACTAATTGAACATGATTTAAATTGCTGGGTTATGAATGTGGTTCCTGTTAGCGGCCCCAACACATTACCTGTTATATATGACCGTGGACTCATTGGAGTAATGCATGACTGGTGTGAACCATTTGATACATACCCACGGACATACGATTTATTGCATGCAGCCAGCCTTTTCTCTATTGAGAAAAAAAGATGTGATATCTCCACAATCATGCTTGAGATGGACCGGATGCTAAGACCTGGTGGTCGTGCATACATCCGTGACTCAATTTCTGTCATGAATGAACTTCAAGCAATCGGAAAAGCCATGGGCTGGCGCGTCAATTTACGAGACACATCTGAGGGGCCTCATGCAAGTTATAGACTCTTTATTGGCGAAAAACGCCTCTTGCATAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

662

Amino Acids

74.5

Weight (kDa)

6.59

Isoelectric Point (pI)

36.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	153 - 656	2e-228	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25	PF13649	273 - 365	5.4e-06	Methyltransferase domain
Methyltransf_11	PF08241	274 - 368	1.1e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39750 AT5G06050
fragaria_vesca	FvH4_7g23080 FvH4_7g23080
malus_domestica	MD01G1139400.v1.1 MD07G1203800.v1.1
prunus_persica	Prupe.2G239900_v2.0.a1
pyrus_communis	pycom01g16000 pycom07g19040
rosa_chinensis	RchiOBHm_Chr1g0368331
rosa_laevigata	RLG00000027219
rosa_roxburghii	Rroxscaffold_4G00288640
rosa_rugosa	Rorug01G0341200 Rorug01G0341200
rosa_samantha	Rh1AG347800 Rh1BG310800 Rh1CG326200 Rh1DG342600
rosa_wichuraiana	Rw1G030960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1125
AasI	GACNNNNNNGTC	1 cut(s)	1830
AatII	GACGTC	1 cut(s)	906
AccB1I	GGYRCC	2 cut(s)	302, 577
AccB7I	CCANNNNNTGG	1 cut(s)	1899
AccBSI	CCGCTC	3 cut(s)	402, 523, 532
AccI	GTMKAC	1 cut(s)	291
AccII	CGCG	4 cut(s)	868, 939, 1180, 1905
AclWI	GGATC	4 cut(s)	37, 293, 332, 1286
AcoI	YGGCCR	2 cut(s)	367, 1388
AcuI	CTGAAG	2 cut(s)	1352, 1406
AcyI	GRCGYC	2 cut(s)	578, 903
AfaI	GTAC	4 cut(s)	461, 645, 730, 986
AfiI	CCNNNNNNNGG	8 cut(s)	548, 625, 725, 994, 1361, 1416, 1640, 1899
AgsI	TTSAA	6 cut(s)	199, 562, 640, 923, 1598, 1877
AjnI	CCWGG	1 cut(s)	1825
AloI	GAACNNNNNNTCC	4 cut(s)	1264, 1296, 1494, 1526
AluBI	AGCT	8 cut(s)	124, 230, 436, 588, 1248, 1462, 1565, 1586
AluI	AGCT	8 cut(s)	124, 230, 436, 588, 1248, 1462, 1565, 1586
Alw21I	GWGCWC	2 cut(s)	157, 438
Alw26I	GTCTC	4 cut(s)	172, 178, 255, 1911
AlwI	GGATC	4 cut(s)	37, 293, 332, 1286
AoxI	GGCC	5 cut(s)	367, 608, 1388, 1642, 1931
ApeKI	GCWGC	8 cut(s)	28, 121, 959, 1109, 1248, 1583, 1586, 1748
ArsI	GACNNNNNNTTYG	2 cut(s)	779, 811
Asp700I	GAANNNNTTC	1 cut(s)	1137
AspLEI	GCGC	3 cut(s)	580, 939, 1905
AspS9I	GGNCC	8 cut(s)	105, 114, 271, 781, 1393, 1643, 1810, 1931
AsuC2I	CCSGG	3 cut(s)	542, 620, 720
AsuHPI	GGTGA	6 cut(s)	70, 127, 427, 784, 965, 1165
AvaII	GGWCC	6 cut(s)	105, 114, 271, 781, 1393, 1810
BanI	GGYRCC	2 cut(s)	302, 577
BanII	GRGCYC	2 cut(s)	438, 599
BauI	CACGAG	2 cut(s)	145, 1041
Bbv12I	GWGCWC	2 cut(s)	157, 438
BbvI	GCAGC	8 cut(s)	15, 133, 971, 1121, 1235, 1573, 1595, 1760
BccI	CCATC	5 cut(s)	64, 104, 746, 1043, 1801
BceAI	ACGGC	2 cut(s)	149, 525
BciT130I	CCWGG	1 cut(s)	1827
BciVI	GTATCC	1 cut(s)	1088
BcnI	CCSGG	3 cut(s)	542, 620, 720
BcoDI	GTCTC	4 cut(s)	172, 178, 255, 1911
BfaI	CTAG	3 cut(s)	185, 227, 1142
BfoI	RGCGCY	1 cut(s)	581
BfuI	GTATCC	1 cut(s)	1088
Bme1390I	CCNGG	4 cut(s)	542, 620, 720, 1827
Bme18I	GGWCC	6 cut(s)	105, 114, 271, 781, 1393, 1810
BmgT120I	GGNCC	8 cut(s)	105, 114, 271, 781, 1393, 1643, 1810, 1931
BmrFI	CCNGG	4 cut(s)	542, 620, 720, 1827
BmsI	GCATC	5 cut(s)	1068, 1152, 1350, 1429, 1806
Bpu10I	CCTNAGC	1 cut(s)	1082
BpuEI	CTTGAG	3 cut(s)	953, 1085, 1823
BpuMI	CCSGG	3 cut(s)	542, 620, 720
BsaBI	GATNNNNATC	1 cut(s)	336
BsaHI	GRCGYC	2 cut(s)	578, 903
BsaJI	CCNNGG	5 cut(s)	507, 605, 1672, 1724, 1893
BsaWI	WCCGGW	1 cut(s)	1812
BsaXI	ACNNNNNCTCC	6 cut(s)	214, 244, 1264, 1294, 1494, 1524
Bsc4I	CCNNNNNNNGG	8 cut(s)	548, 625, 725, 994, 1361, 1416, 1640, 1899
Bse118I	RCCGGY	1 cut(s)	1234
Bse1I	ACTGG	4 cut(s)	107, 1040, 1118, 1703
Bse3DI	GCAATG	4 cut(s)	22, 535, 957, 1012
Bse8I	GATNNNNATC	1 cut(s)	336
BseBI	CCWGG	1 cut(s)	1827
BseDI	CCNNGG	5 cut(s)	507, 605, 1672, 1724, 1893
BseGI	GGATG	7 cut(s)	628, 829, 836, 1083, 1270, 1821, 1842
BseJI	GATNNNNATC	1 cut(s)	336
BseLI	CCNNNNNNNGG	8 cut(s)	548, 625, 725, 994, 1361, 1416, 1640, 1899
BseMI	GCAATG	4 cut(s)	22, 535, 957, 1012
BseMII	CTCAG	2 cut(s)	1096, 1917
BseNI	ACTGG	4 cut(s)	107, 1040, 1118, 1703
BseRI	GAGGAG	1 cut(s)	1160
BseXI	GCAGC	8 cut(s)	15, 133, 971, 1121, 1235, 1573, 1595, 1760
BseYI	CCCAGC	3 cut(s)	117, 1188, 1614
BsgI	GTGCAG	1 cut(s)	1572
Bsh1236I	CGCG	4 cut(s)	868, 939, 1180, 1905
BshFI	GGCC	5 cut(s)	369, 610, 1390, 1644, 1933
BshNI	GGYRCC	2 cut(s)	302, 577
BsiHKAI	GWGCWC	2 cut(s)	157, 438
BsiSI	CCGG	7 cut(s)	542, 575, 619, 719, 1235, 1391, 1813
BslFI	GGGAC	3 cut(s)	100, 391, 767
BslI	CCNNNNNNNGG	8 cut(s)	548, 625, 725, 994, 1361, 1416, 1640, 1899
BsmAI	GTCTC	4 cut(s)	172, 178, 255, 1911
BsmBI	CGTCTC	3 cut(s)	172, 178, 255
BsmFI	GGGAC	3 cut(s)	100, 391, 767
BsnI	GGCC	5 cut(s)	369, 610, 1390, 1644, 1933
Bsp1286I	GDGCHC	3 cut(s)	157, 438, 599
Bsp143I	GATC	5 cut(s)	42, 285, 337, 1291, 1327
Bsp19I	CCATGG	2 cut(s)	605, 1893
Bsp68I	TCGCGA	1 cut(s)	868
BspANI	GGCC	5 cut(s)	369, 610, 1390, 1644, 1933
BspCNI	CTCAG	2 cut(s)	1095, 1918
BspFNI	CGCG	4 cut(s)	868, 939, 1180, 1905
BspHI	TCATGA	1 cut(s)	1863
BspPI	GGATC	4 cut(s)	37, 293, 332, 1286
BspT107I	GGYRCC	2 cut(s)	302, 577
BsrBI	CCGCTC	3 cut(s)	402, 523, 532
BsrDI	GCAATG	4 cut(s)	22, 535, 957, 1012
BsrFI	RCCGGY	1 cut(s)	1234
BsrI	ACTGG	4 cut(s)	107, 1040, 1118, 1703
BssAI	RCCGGY	1 cut(s)	1234
BssECI	CCNNGG	5 cut(s)	507, 605, 1672, 1724, 1893
BssMI	GATC	5 cut(s)	42, 285, 337, 1291, 1327
BssNI	GRCGYC	2 cut(s)	578, 903
BssSI	CACGAG	2 cut(s)	145, 1041
BssT1I	CCWWGG	2 cut(s)	605, 1893
Bst2BI	CACGAG	2 cut(s)	145, 1041
Bst2UI	CCWGG	1 cut(s)	1827
Bst4CI	ACNGT	5 cut(s)	382, 735, 977, 1397, 1673
Bst6I	CTCTTC	2 cut(s)	134, 440
BstACI	GRCGYC	2 cut(s)	578, 903
BstAPI	GCANNNNNTGC	2 cut(s)	965, 1162
BstC8I	GCNNGC	7 cut(s)	521, 948, 1339, 1567, 1746, 1753, 1901
BstDEI	CTNAG	4 cut(s)	774, 1082, 1820, 1926
BstDSI	CCRYGG	5 cut(s)	507, 605, 1672, 1724, 1893
BstF5I	GGATG	7 cut(s)	628, 829, 836, 1083, 1270, 1821, 1842
BstFNI	CGCG	4 cut(s)	868, 939, 1180, 1905
BstH2I	RGCGCY	1 cut(s)	581
BstHHI	GCGC	3 cut(s)	580, 939, 1905
BstKTI	GATC	5 cut(s)	45, 288, 340, 1294, 1330
BstMAI	GTCTC	4 cut(s)	172, 178, 255, 1911
BstMBI	GATC	5 cut(s)	42, 285, 337, 1291, 1327
BstMWI	GCNNNNNNNGC	9 cut(s)	161, 529, 594, 943, 956, 965, 1109, 1162, 1968
BstNI	CCWGG	1 cut(s)	1827
BstNSI	RCATGY	3 cut(s)	809, 1341, 1748
BstSCI	CCNGG	4 cut(s)	540, 618, 718, 1825
BstUI	CGCG	4 cut(s)	868, 939, 1180, 1905
BstV1I	GCAGC	8 cut(s)	15, 133, 971, 1121, 1235, 1573, 1595, 1760
BstXI	CCANNNNNNTGG	1 cut(s)	606
BsuI	GTATCC	1 cut(s)	1088
BsuRI	GGCC	5 cut(s)	369, 610, 1390, 1644, 1933
BtgI	CCRYGG	5 cut(s)	507, 605, 1672, 1724, 1893
BtsCI	GGATG	7 cut(s)	628, 829, 836, 1083, 1270, 1821, 1842
BtsI	GCAGTG	1 cut(s)	1157
BtsIMutI	CAGTG	2 cut(s)	100, 1157
BtuMI	TCGCGA	1 cut(s)	868
Cac8I	GCNNGC	7 cut(s)	521, 948, 1339, 1567, 1746, 1753, 1901
CciI	TCATGA	1 cut(s)	1863
CfoI	GCGC	3 cut(s)	580, 939, 1905
Cfr10I	RCCGGY	1 cut(s)	1234
Cfr13I	GGNCC	8 cut(s)	105, 114, 271, 781, 1393, 1643, 1810, 1931
CpoI	CGGWCCG	1 cut(s)	1393
CseI	GACGC	1 cut(s)	1894
CsiI	ACCWGGT	1 cut(s)	1825
Csp6I	GTAC	4 cut(s)	460, 644, 729, 985
CspCI	CAANNNNNGTGG	4 cut(s)	222, 257, 642, 677
CspI	CGGWCCG	1 cut(s)	1393
CviQI	GTAC	4 cut(s)	460, 644, 729, 985
DdeI	CTNAG	4 cut(s)	774, 1082, 1820, 1926
DinI	GGCGCC	1 cut(s)	579
DpnI	GATC	5 cut(s)	44, 287, 339, 1293, 1329
DpnII	GATC	5 cut(s)	42, 285, 337, 1291, 1327
DraI	TTTAAA	3 cut(s)	1468, 1521, 1608
DrdI	GACNNNNNNGTC	1 cut(s)	1830
DseDI	GACNNNNNNGTC	1 cut(s)	1830
EaeI	YGGCCR	2 cut(s)	367, 1388
Eam1104I	CTCTTC	2 cut(s)	134, 440
EarI	CTCTTC	2 cut(s)	134, 440
EciI	GGCGGA	4 cut(s)	151, 196, 243, 401
Ecl136II	GAGCTC	1 cut(s)	436
Eco130I	CCWWGG	2 cut(s)	605, 1893
Eco24I	GRGCYC	2 cut(s)	438, 599
Eco32I	GATATC	1 cut(s)	1786
Eco47I	GGWCC	6 cut(s)	105, 114, 271, 781, 1393, 1810
Eco53kI	GAGCTC	1 cut(s)	436
Eco57I	CTGAAG	2 cut(s)	1352, 1406
EcoICRI	GAGCTC	1 cut(s)	436
EcoO109I	RGGNCCY	2 cut(s)	114, 1931
EcoRII	CCWGG	1 cut(s)	1825
EcoRV	GATATC	1 cut(s)	1786
EcoT14I	CCWWGG	2 cut(s)	605, 1893
EcoT22I	ATGCAT	2 cut(s)	1343, 1695
EcoT38I	GRGCYC	2 cut(s)	438, 599
EgeI	GGCGCC	1 cut(s)	579
EheI	GGCGCC	1 cut(s)	579
ErhI	CCWWGG	2 cut(s)	605, 1893
Esp3I	CGTCTC	3 cut(s)	172, 178, 255
FalI	AAGNNNNNCTT	2 cut(s)	1149, 1181
FaqI	GGGAC	3 cut(s)	100, 391, 767
FauI	CCCGC	1 cut(s)	395
FblI	GTMKAC	1 cut(s)	291
FokI	GGATG	7 cut(s)	635, 836, 843, 1090, 1277, 1828, 1829
FriOI	GRGCYC	2 cut(s)	438, 599
FspBI	CTAG	3 cut(s)	185, 227, 1142
GlaI	GCGC	3 cut(s)	579, 938, 1904
GsaI	CCCAGC	3 cut(s)	121, 1192, 1618
HaeII	RGCGCY	1 cut(s)	581
HaeIII	GGCC	5 cut(s)	369, 610, 1390, 1644, 1933
HapII	CCGG	7 cut(s)	542, 575, 619, 719, 1235, 1391, 1813
HgaI	GACGC	1 cut(s)	1894
HhaI	GCGC	3 cut(s)	580, 939, 1905
Hin1I	GRCGYC	2 cut(s)	578, 903
Hin6I	GCGC	3 cut(s)	578, 937, 1903
HinP1I	GCGC	3 cut(s)	578, 937, 1903
HincII	GTYRAC	2 cut(s)	292, 1240
HindII	GTYRAC	2 cut(s)	292, 1240
HinfI	GANTC	7 cut(s)	33, 919, 1039, 1475, 1677, 1850, 1950
HpaI	GTTAAC	1 cut(s)	1240
HpaII	CCGG	7 cut(s)	542, 575, 619, 719, 1235, 1391, 1813
HphI	GGTGA	6 cut(s)	70, 127, 427, 784, 965, 1165
Hpy166II	GTNNAC	5 cut(s)	292, 731, 1240, 1676, 1706
Hpy8I	GTNNAC	5 cut(s)	292, 731, 1240, 1676, 1706
Hpy99I	CGWCG	2 cut(s)	293, 296
HpyAV	CCTTC	4 cut(s)	91, 805, 1262, 1327
HpyCH4III	ACNGT	5 cut(s)	382, 735, 977, 1397, 1673
HpyCH4IV	ACGT	3 cut(s)	642, 903, 1548
HpyF10VI	GCNNNNNNNGC	9 cut(s)	161, 529, 594, 943, 956, 965, 1109, 1162, 1968
HpyF3I	CTNAG	4 cut(s)	774, 1082, 1820, 1926
HpySE526I	ACGT	3 cut(s)	642, 903, 1548
Hsp92I	GRCGYC	2 cut(s)	578, 903
HspAI	GCGC	3 cut(s)	578, 937, 1903
KasI	GGCGCC	1 cut(s)	577
KflI	GGGWCCC	1 cut(s)	114
KspAI	GTTAAC	1 cut(s)	1240
Kzo9I	GATC	5 cut(s)	42, 285, 337, 1291, 1327
LmnI	GCTCC	5 cut(s)	235, 399, 746, 1147, 1459
Lsp1109I	GCAGC	8 cut(s)	15, 133, 971, 1121, 1235, 1573, 1595, 1760
LweI	GCATC	5 cut(s)	1068, 1152, 1350, 1429, 1806
MabI	ACCWGGT	1 cut(s)	1825
MaeI	CTAG	3 cut(s)	185, 227, 1142
MaeII	ACGT	3 cut(s)	642, 903, 1548
MaeIII	GTNAC	4 cut(s)	76, 755, 1378, 1847
MalI	GATC	5 cut(s)	44, 287, 339, 1293, 1329
MbiI	CCGCTC	3 cut(s)	402, 523, 532
MboI	GATC	5 cut(s)	42, 285, 337, 1291, 1327
MboII	GAAGA	9 cut(s)	76, 121, 124, 457, 628, 677, 712, 1145, 1540
MhlI	GDGCHC	3 cut(s)	157, 438, 599
Mly113I	GGCGCC	1 cut(s)	578
MlyI	GAGTC	4 cut(s)	1033, 1671, 1844, 1944
MmeI	TCCRAC	2 cut(s)	808, 1480
Mph1103I	ATGCAT	2 cut(s)	1343, 1695
MroXI	GAANNNNTTC	1 cut(s)	1137
MslI	CAYNNNNRTG	1 cut(s)	1796
MspA1I	CMGCKG	2 cut(s)	1248, 1586
MspI	CCGG	7 cut(s)	542, 575, 619, 719, 1235, 1391, 1813
MspR9I	CCNGG	4 cut(s)	542, 620, 720, 1827
MvaI	CCWGG	1 cut(s)	1827
MvnI	CGCG	4 cut(s)	868, 939, 1180, 1905
MwoI	GCNNNNNNNGC	9 cut(s)	161, 529, 594, 943, 956, 965, 1109, 1162, 1968
NarI	GGCGCC	1 cut(s)	578
NciI	CCSGG	3 cut(s)	542, 620, 720
NcoI	CCATGG	2 cut(s)	605, 1893
NdeII	GATC	5 cut(s)	42, 285, 337, 1291, 1327
NmeAIII	GCCGAG	1 cut(s)	176
NmuCI	GTSAC	3 cut(s)	76, 1378, 1847
NruI	TCGCGA	1 cut(s)	868
NsiI	ATGCAT	2 cut(s)	1343, 1695
NspI	RCATGY	3 cut(s)	809, 1341, 1748
PaeI	GCATGC	2 cut(s)	1341, 1748
PagI	TCATGA	1 cut(s)	1863
PcsI	WCGNNNNNNNCGW	2 cut(s)	288, 333
PdmI	GAANNNNTTC	1 cut(s)	1137
PfeI	GAWTC	3 cut(s)	33, 919, 1475
PflMI	CCANNNNNTGG	1 cut(s)	1899
PfoI	TCCNGGA	1 cut(s)	718
PleI	GAGTC	4 cut(s)	1033, 1671, 1844, 1944
PluTI	GGCGCC	1 cut(s)	581
PpsI	GAGTC	4 cut(s)	1033, 1671, 1844, 1944
PpuMI	RGGWCCY	1 cut(s)	114
PsiI	TTATAA	1 cut(s)	1125
Psp124BI	GAGCTC	1 cut(s)	438
Psp5II	RGGWCCY	1 cut(s)	114
Psp6I	CCWGG	1 cut(s)	1825
PspFI	CCCAGC	3 cut(s)	117, 1188, 1614
PspGI	CCWGG	1 cut(s)	1825
PspPI	GGNCC	8 cut(s)	105, 114, 271, 781, 1393, 1643, 1810, 1931
PspPPI	RGGWCCY	1 cut(s)	114
PvuII	CAGCTG	2 cut(s)	1248, 1586
RruI	TCGCGA	1 cut(s)	868
RsaI	GTAC	4 cut(s)	461, 645, 730, 986
RsaNI	GTAC	4 cut(s)	460, 644, 729, 985
RseI	CAYNNNNRTG	1 cut(s)	1796
Rsr2I	CGGWCCG	1 cut(s)	1393
RsrII	CGGWCCG	1 cut(s)	1393
SacI	GAGCTC	1 cut(s)	438
SalI	GTCGAC	1 cut(s)	290
Sau3AI	GATC	5 cut(s)	42, 285, 337, 1291, 1327
Sau96I	GGNCC	8 cut(s)	105, 114, 271, 781, 1393, 1643, 1810, 1931
SchI	GAGTC	4 cut(s)	1033, 1671, 1844, 1944
ScrFI	CCNGG	4 cut(s)	542, 620, 720, 1827
SduI	GDGCHC	3 cut(s)	157, 438, 599
SexAI	ACCWGGT	1 cut(s)	1825
SfaNI	GCATC	5 cut(s)	1068, 1152, 1350, 1429, 1806
SfoI	GGCGCC	1 cut(s)	579
SgrDI	CGTCGACG	1 cut(s)	290
SinI	GGWCC	6 cut(s)	105, 114, 271, 781, 1393, 1810
SmiI	ATTTAAAT	1 cut(s)	1608
SmiMI	CAYNNNNRTG	1 cut(s)	1796
SmlI	CTYRAG	3 cut(s)	932, 1064, 1802
SmoI	CTYRAG	3 cut(s)	932, 1064, 1802
SphI	GCATGC	2 cut(s)	1341, 1748
SspDI	GGCGCC	1 cut(s)	577
SspMI	CTAG	3 cut(s)	185, 227, 1142
SstI	GAGCTC	1 cut(s)	438
StyD4I	CCNGG	4 cut(s)	540, 618, 718, 1825
StyI	CCWWGG	2 cut(s)	605, 1893
SwaI	ATTTAAAT	1 cut(s)	1608
TaaI	ACNGT	5 cut(s)	382, 735, 977, 1397, 1673
TaiI	ACGT	3 cut(s)	645, 906, 1551
TaqI	TCGA	4 cut(s)	291, 321, 336, 432
TaqII	GACCGA	1 cut(s)	788
TatI	WGTACW	1 cut(s)	459
TauI	GCSGC	6 cut(s)	259, 262, 369, 535, 1183, 1644
TfiI	GAWTC	3 cut(s)	33, 919, 1475
TscAI	CASTG	2 cut(s)	107, 1157
TseFI	GTSAC	3 cut(s)	76, 1378, 1847
TseI	GCWGC	8 cut(s)	28, 121, 959, 1109, 1248, 1583, 1586, 1748
Tsp45I	GTSAC	3 cut(s)	76, 1378, 1847
TspDTI	ATGAA	5 cut(s)	1146, 1281, 1637, 1880, 1884
TspGWI	ACGGA	4 cut(s)	160, 237, 1741, 1835
TspRI	CASTG	2 cut(s)	107, 1157
Van91I	CCANNNNNTGG	1 cut(s)	1899
VpaK11BI	GGWCC	6 cut(s)	105, 114, 271, 781, 1393, 1810
XbaI	TCTAGA	1 cut(s)	1141
XceI	RCATGY	3 cut(s)	809, 1341, 1748
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1314, 1496
XmiI	GTMKAC	1 cut(s)	291
XmnI	GAANNNNTTC	1 cut(s)	1137
XspI	CTAG	3 cut(s)	185, 227, 1142
ZraI	GACGTC	1 cut(s)	904
Zsp2I	ATGCAT	2 cut(s)	1343, 1695

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.