Rroxscaffold_4G00288640

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0011819
Physical Location & Seq
Forward (+)
9338417 .. 9342989
4573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00288640.1

Sequence Viewer

Length: 2025 bp
ATGGGATATCTCGGGAATGGCGATTGGTTGAGGATCCCATTGATCATCAAAATATGGGGGTTTATCATGATCTCCGTCACCTTCTTCTACTTGGGCAAGAACTGGACCGGTGGGGCCCAGCAGCTCCTCTCCTTCACCACCACCACCGCCGCCACGCGCCGATCTCCTCCTTCCGCCACGGTCTCCATCTCCCCCAATCTCAACAAGCCTTTCAACCTCTCCGCCCTCCTCAACGAAACCCTAGCCCCGCCTCCGCCGCCTACTCCATCTCCTCCGCCCCCCGCGCCGCCGCCGTCGGACGTGATCCAGCGGTTCGGGATCGTGGACGAGAACGGCACCATGGCCGAAGATTTCGAGGTCGGCGAGTTCGATCCCGATGAGGTGGTGGAGAATTGGAGCGGCCAGAGTGGGACGGCGGCGGCGGCGGAGAGTGACGGGAGCGGGTCGGGTAGGTTCAGTGGGAAAAGGTTTGAGCTTTGTCGTAACGGTATGAGCGAGTACATACCGTGTTTGGATAATGTGGTGGCCATTAGGAGGCTCAAATCCACCGATAAAGGCGAGCGCTTTGAGCGGCATTGTCCGGAATCCGGCAAGGGTTTGAATTGCTTGGTTCCGCCGCCCAAGAATTACAAGAGCCCCATTCCATGGCCAAAGAGCCGCGATGAGGTTTGGTTCTACAATGTACCTCATACTCGTCTGGTTGAGGATAAAGGGGGTCAGAACTGGATTACACGAGCAAAGAATAAGTTTAAGTTTCCGGGAGGTGGGACACAGTTTATACATGGGGCAAATGAGTACTTGGATCATATTTCTAAGATGATCCCTGACATCACATTTGGTCACCATATCCGAGTTGCATTGGATGTTGGATGTGGAGTTGCAAGTTTTGGTGCGTACCTGCTGCAGCGGAATGTCATCACTATGTCAATTGCTCCGAAAGATGTCCATGAGAACCAGATACAATTTGCTCTTGAGCGTGGAGTGCCCGCAATGGTAGCAGCATTTGCTACTCGCCGTTTATTGTACCCAAGTCAGGCATTTGACTTGATCCATTGCTCACGATGCAGGATTAATTGGACTCGTGACGATGGGATATTGCTACTTGAGGTTAATAGGATGCTCCGGGCAGGAGGATACTTTGTTTGGGCAGCACAGCCAGTTTATAAGCATGAAGAAGTCCTAGAGGAGCAGTGGCAAGAGATGCTTAACCTCACGACCCGTCTTTGCTGGGAGTTTGTGAAAAAGGATGGCTATGTTGCCGTATGGCAGAAGCCAGTTAACAACAACTGCTATCTAGGTCGGGATGCTGAAACCAAACCTCCATTATGCGACTCAAGTGATGACCCGGACAAAGTTTGGTATGTTGATCTGAAGGCATGCATAACAAAACTCCCTGAAAATGGTTATGGAGCAAATGTCACAACATGGCCAGACCGCATGCAAACTCCCCCTGATAGGCTTCAGAGCATCCAGTTTGATGCCTATATATCCAGAAAGGAGCTCTTCAAGGCAGAATCTAAATATTGGCATGAAATAATTGAGGGCTATGTCCGTGCTTTTAAATGGAAGAAGTTAAGATTAAGAAATGTAATGGATATGAGAGCTGGCTTTGGAGGATTTGCAGTGGCACTAATGGAACATAACTTAAATTCCTGGGTTATGAATGTGGTTCCTGTTAGCGGCCCCAACACCTTGCCTGTTATATATGACCGTGGATTTATTGGAGTTATGCATGACTGGTGTGAGCCATTTGATACATACCCAAGAACCTATGATTTATTACATGCAGCTGGCCTCTTCTCTATTGAGAAGAAAAGGTGCAATATGTCAACAATCATGCTTGAGATGGACCGTATGCTAAGACCAGGTGGACGTGTATACATCCGTGATGCTCTTGCGGTCATGGATGAGCTTCAAGCGATTGGAAATGCCATGGGCTGGAAAGTTAATTTGCGAGACACGTCTGAGGGTCCTCATGCAAGCTATAGGCTCTTCATGGCTGAAAAACGCCTCTTGCATAGTTGA

Protein Analysis

674

Amino Acids

76.26

Weight (kDa)

7.87

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 166 - 669 3.6e-231 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 287 - 381 7.5e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39750 AT5G06050
fragaria_vesca FvH4_7g23080 FvH4_7g23080
malus_domestica MD01G1139400.v1.1 MD07G1203800.v1.1
prunus_persica Prupe.2G239900_v2.0.a1
pyrus_communis pycom01g16000 pycom07g19040
rosa_chinensis RchiOBHm_Chr1g0368331
rosa_laevigata RLG00000027219
rosa_roxburghii Rroxscaffold_4G00288640
rosa_rugosa Rorug01G0341200 Rorug01G0341200
rosa_samantha Rh1AG347800 Rh1BG310800 Rh1CG326200 Rh1DG342600
rosa_wichuraiana Rw1G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1164
Acc36I ACCTGC 1 cut(s) 906
AccB1I GGYRCC 1 cut(s) 335
AccB7I CCANNNNNTGG 2 cut(s) 645, 1938
AccBSI CCGCTC 3 cut(s) 399, 441, 571
AccI GTMKAC 1 cut(s) 1878
AccII CGCG 3 cut(s) 157, 284, 660
AccIII TCCGGA 1 cut(s) 580
AclWI GGATC 8 cut(s) 28, 41, 298, 326, 365, 810, 814, 1042
AcoI YGGCCR 5 cut(s) 342, 400, 525, 647, 1427
AcsI RAATTY 1 cut(s) 1648
AcuI CTGAAG 2 cut(s) 1391, 1445
AfaI GTAC 5 cut(s) 500, 684, 797, 896, 1025
AfeI AGCGCT 1 cut(s) 563
AflIII ACRYGT 2 cut(s) 1873, 1959
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 4 cut(s) 214, 601, 1507, 1916
AjiI CACGTC 3 cut(s) 301, 1874, 1962
AjnI CCWGG 2 cut(s) 1652, 1864
AluBI AGCT 7 cut(s) 124, 475, 1501, 1604, 1790, 1912, 1983
AluI AGCT 7 cut(s) 124, 475, 1501, 1604, 1790, 1912, 1983
Alw21I GWGCWC 1 cut(s) 1503
Alw26I GTCTC 2 cut(s) 187, 1950
AlwI GGATC 8 cut(s) 28, 41, 298, 326, 365, 810, 814, 1042
Ama87I CYCGRG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 580
Aor51HI AGCGCT 1 cut(s) 563
AoxI GGCC 8 cut(s) 114, 342, 400, 525, 647, 1427, 1681, 1792
ApaI GGGCCC 1 cut(s) 118
ApeKI GCWGC 6 cut(s) 121, 901, 904, 998, 1148, 1787
ApoI RAATTY 1 cut(s) 1648
ArsI GACNNNNNNTTYG 2 cut(s) 818, 850
AseI ATTAAT 1 cut(s) 1071
AsiGI ACCGGT 1 cut(s) 107
AspLEI GCGC 3 cut(s) 159, 286, 564
AspS9I GGNCC 6 cut(s) 105, 114, 115, 1682, 1849, 1970
AsuC2I CCSGG 3 cut(s) 759, 1124, 1346
AsuHPI GGTGA 3 cut(s) 70, 127, 833
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 3 cut(s) 105, 1849, 1970
BaeGI GKGCMC 2 cut(s) 118, 987
BalI TGGCCA 3 cut(s) 527, 649, 1429
BamHI GGATCC 1 cut(s) 33
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 3 cut(s) 118, 638, 1503
BauI CACGAG 2 cut(s) 732, 1080
Bbv12I GWGCWC 1 cut(s) 1503
BbvI GCAGC 6 cut(s) 133, 888, 916, 1010, 1160, 1799
BccI CCATC 5 cut(s) 194, 274, 1082, 1241, 1840
BceAI ACGGC 5 cut(s) 277, 349, 429, 999, 1244
BciT130I CCWGG 2 cut(s) 1654, 1866
BciVI GTATCC 1 cut(s) 1127
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 3 cut(s) 759, 1124, 1346
BcoDI GTCTC 2 cut(s) 187, 1950
BfaI CTAG 3 cut(s) 242, 1181, 1295
BfmI CTRYAG 2 cut(s) 902, 1984
BfoI RGCGCY 1 cut(s) 565
BfuAI ACCTGC 1 cut(s) 906
BfuI GTATCC 1 cut(s) 1127
BmcAI AGTACT 1 cut(s) 797
Bme1390I CCNGG 5 cut(s) 759, 1124, 1346, 1654, 1866
Bme18I GGWCC 3 cut(s) 105, 1849, 1970
BmeT110I CYCGRG 1 cut(s) 11
BmgBI CACGTC 3 cut(s) 301, 1874, 1962
BmgT120I GGNCC 6 cut(s) 105, 114, 115, 1682, 1849, 1970
BmiI GGNNCC 8 cut(s) 35, 115, 116, 337, 612, 1671, 1684, 1971
BmrFI CCNGG 5 cut(s) 759, 1124, 1346, 1654, 1866
BmsI GCATC 7 cut(s) 1052, 1107, 1191, 1294, 1468, 1476, 1879
BpuEI CTTGAG 4 cut(s) 992, 1124, 1318, 1862
BpuMI CCSGG 3 cut(s) 759, 1124, 1346
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 6 cut(s) 177, 339, 644, 1653, 1711, 1932
BsaWI WCCGGW 2 cut(s) 107, 580
BsaXI ACNNNNNCTCC 6 cut(s) 253, 283, 1303, 1333, 1401, 1431
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 6 cut(s) 107, 728, 1157, 1274, 1471, 1742
Bse3DI GCAATG 2 cut(s) 996, 1051
BseAI TCCGGA 1 cut(s) 580
BseBI CCWGG 2 cut(s) 1654, 1866
BseDI CCNNGG 6 cut(s) 177, 339, 644, 1653, 1711, 1932
BseGI GGATG 8 cut(s) 868, 875, 1122, 1252, 1309, 1467, 1881, 1912
BseMI GCAATG 2 cut(s) 996, 1051
BseMII CTCAG 1 cut(s) 1956
BseNI ACTGG 6 cut(s) 107, 728, 1157, 1274, 1471, 1742
BseRI GAGGAG 5 cut(s) 116, 156, 218, 261, 1199
BseSI GKGCMC 2 cut(s) 118, 987
BseXI GCAGC 6 cut(s) 133, 888, 916, 1010, 1160, 1799
BseYI CCCAGC 2 cut(s) 117, 1227
Bsh1236I CGCG 3 cut(s) 157, 284, 660
BshFI GGCC 8 cut(s) 116, 344, 402, 527, 649, 1429, 1683, 1794
BshNI GGYRCC 1 cut(s) 335
BshTI ACCGGT 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 1503
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 6 cut(s) 108, 581, 588, 758, 1123, 1346
BslFI GGGAC 2 cut(s) 424, 781
BsmAI GTCTC 2 cut(s) 187, 1950
BsmFI GGGAC 2 cut(s) 424, 781
BsnI GGCC 8 cut(s) 116, 344, 402, 527, 649, 1429, 1683, 1794
Bso31I GGTCTC 1 cut(s) 187
BsoBI CYCGRG 1 cut(s) 11
Bsp120I GGGCCC 1 cut(s) 114
Bsp1286I GDGCHC 4 cut(s) 118, 638, 987, 1503
Bsp13I TCCGGA 1 cut(s) 580
Bsp19I CCATGG 3 cut(s) 339, 644, 1932
BspANI GGCC 8 cut(s) 116, 344, 402, 527, 649, 1429, 1683, 1794
BspCNI CTCAG 1 cut(s) 1957
BspEI TCCGGA 1 cut(s) 580
BspFNI CGCG 3 cut(s) 157, 284, 660
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 8 cut(s) 35, 115, 116, 337, 612, 1671, 1684, 1971
BspMAI CTGCAG 1 cut(s) 906
BspMI ACCTGC 1 cut(s) 906
BspPI GGATC 8 cut(s) 28, 41, 298, 326, 365, 810, 814, 1042
BspQI GCTCTTC 2 cut(s) 1508, 1997
BspT107I GGYRCC 1 cut(s) 335
BspTNI GGTCTC 1 cut(s) 187
BsrBI CCGCTC 3 cut(s) 399, 441, 571
BsrDI GCAATG 2 cut(s) 996, 1051
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 6 cut(s) 107, 728, 1157, 1274, 1471, 1742
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 6 cut(s) 177, 339, 644, 1653, 1711, 1932
BssNAI GTATAC 1 cut(s) 1879
BssSI CACGAG 2 cut(s) 732, 1080
BssT1I CCWWGG 3 cut(s) 339, 644, 1932
Bst1107I GTATAC 1 cut(s) 1879
Bst2BI CACGAG 2 cut(s) 732, 1080
Bst2UI CCWGG 2 cut(s) 1654, 1866
Bst4CI ACNGT 6 cut(s) 181, 488, 507, 774, 1712, 1853
Bst6I CTCTTC 3 cut(s) 1508, 1802, 1997
BstAPI GCANNNNNTGC 2 cut(s) 1004, 1201
BstC8I GCNNGC 7 cut(s) 560, 987, 1378, 1439, 1606, 1792, 1981
BstDEI CTNAG 3 cut(s) 813, 1859, 1965
BstDSI CCRYGG 5 cut(s) 177, 339, 644, 1711, 1932
BstEII GGTNACC 1 cut(s) 839
BstF5I GGATG 8 cut(s) 868, 875, 1122, 1252, 1309, 1467, 1881, 1912
BstFNI CGCG 3 cut(s) 157, 284, 660
BstH2I RGCGCY 1 cut(s) 565
BstHHI GCGC 3 cut(s) 159, 286, 564
BstMAI GTCTC 2 cut(s) 187, 1950
BstMWI GCNNNNNNNGC 9 cut(s) 256, 283, 422, 568, 982, 995, 1004, 1062, 1201
BstNI CCWGG 2 cut(s) 1654, 1866
BstNSI RCATGY 3 cut(s) 1380, 1441, 1787
BstPI GGTNACC 1 cut(s) 839
BstSCI CCNGG 5 cut(s) 757, 1122, 1344, 1652, 1864
BstSFI CTRYAG 2 cut(s) 902, 1984
BstSLI GKGCMC 2 cut(s) 118, 987
BstUI CGCG 3 cut(s) 157, 284, 660
BstV1I GCAGC 6 cut(s) 133, 888, 916, 1010, 1160, 1799
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BstZ17I GTATAC 1 cut(s) 1879
BsuI GTATCC 1 cut(s) 1127
BsuRI GGCC 8 cut(s) 116, 344, 402, 527, 649, 1429, 1683, 1794
BtgI CCRYGG 5 cut(s) 177, 339, 644, 1711, 1932
BtgZI GCGATG 1 cut(s) 675
BtrI CACGTC 3 cut(s) 301, 1874, 1962
BtsCI GGATG 8 cut(s) 868, 875, 1122, 1252, 1309, 1467, 1881, 1912
BtsI GCAGTG 2 cut(s) 1196, 1629
BtsIMutI CAGTG 3 cut(s) 463, 1196, 1629
BveI ACCTGC 1 cut(s) 906
Cac8I GCNNGC 7 cut(s) 560, 987, 1378, 1439, 1606, 1792, 1981
CciI TCATGA 1 cut(s) 66
CfoI GCGC 3 cut(s) 159, 286, 564
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 6 cut(s) 105, 114, 115, 1682, 1849, 1970
CsiI ACCWGGT 1 cut(s) 1864
Csp6I GTAC 5 cut(s) 499, 683, 796, 895, 1024
CspAI ACCGGT 1 cut(s) 107
CviQI GTAC 5 cut(s) 499, 683, 796, 895, 1024
DdeI CTNAG 3 cut(s) 813, 1859, 1965
DraI TTTAAA 1 cut(s) 1561
EaeI YGGCCR 5 cut(s) 342, 400, 525, 647, 1427
Eam1104I CTCTTC 3 cut(s) 1508, 1802, 1997
EarI CTCTTC 3 cut(s) 1508, 1802, 1997
EciI GGCGGA 6 cut(s) 163, 211, 243, 264, 440, 603
Ecl136II GAGCTC 1 cut(s) 1501
Eco130I CCWWGG 3 cut(s) 339, 644, 1932
Eco24I GRGCYC 3 cut(s) 118, 638, 1503
Eco31I GGTCTC 1 cut(s) 187
Eco32I GATATC 1 cut(s) 8
Eco47I GGWCC 3 cut(s) 105, 1849, 1970
Eco47III AGCGCT 1 cut(s) 563
Eco53kI GAGCTC 1 cut(s) 1501
Eco57I CTGAAG 2 cut(s) 1391, 1445
Eco88I CYCGRG 1 cut(s) 11
Eco91I GGTNACC 1 cut(s) 839
EcoICRI GAGCTC 1 cut(s) 1501
EcoO109I RGGNCCY 2 cut(s) 114, 1970
EcoO65I GGTNACC 1 cut(s) 839
EcoRII CCWGG 2 cut(s) 1652, 1864
EcoRV GATATC 1 cut(s) 8
EcoT14I CCWWGG 3 cut(s) 339, 644, 1932
EcoT22I ATGCAT 2 cut(s) 1382, 1734
EcoT38I GRGCYC 3 cut(s) 118, 638, 1503
ErhI CCWWGG 3 cut(s) 339, 644, 1932
FalI AAGNNNNNCTT 4 cut(s) 1188, 1220, 1487, 1519
FaqI GGGAC 2 cut(s) 424, 781
FauI CCCGC 4 cut(s) 255, 289, 434, 994
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 1878
FokI GGATG 8 cut(s) 875, 882, 1129, 1259, 1316, 1454, 1868, 1919
FriOI GRGCYC 3 cut(s) 118, 638, 1503
FspBI CTAG 3 cut(s) 242, 1181, 1295
GlaI GCGC 3 cut(s) 158, 285, 563
GsaI CCCAGC 2 cut(s) 121, 1231
HaeII RGCGCY 1 cut(s) 565
HaeIII GGCC 8 cut(s) 116, 344, 402, 527, 649, 1429, 1683, 1794
HapII CCGG 6 cut(s) 108, 581, 588, 758, 1123, 1346
HhaI GCGC 3 cut(s) 159, 286, 564
Hin6I GCGC 3 cut(s) 157, 284, 562
HinP1I GCGC 3 cut(s) 157, 284, 562
HincII GTYRAC 2 cut(s) 1279, 1830
HindII GTYRAC 2 cut(s) 1279, 1830
HinfI GANTC 4 cut(s) 584, 1078, 1331, 1514
HpaI GTTAAC 1 cut(s) 1279
HpaII CCGG 6 cut(s) 108, 581, 588, 758, 1123, 1346
HphI GGTGA 3 cut(s) 70, 127, 833
Hpy166II GTNNAC 5 cut(s) 325, 1279, 1830, 1871, 1879
Hpy188I TCNGA 7 cut(s) 298, 720, 851, 936, 1371, 1464, 1966
Hpy8I GTNNAC 5 cut(s) 325, 1279, 1830, 1871, 1879
Hpy99I CGWCG 1 cut(s) 298
HpyAV CCTTC 4 cut(s) 91, 142, 180, 1366
HpyCH4III ACNGT 6 cut(s) 181, 488, 507, 774, 1712, 1853
HpyCH4IV ACGT 3 cut(s) 300, 1873, 1961
HpyF10VI GCNNNNNNNGC 9 cut(s) 256, 283, 422, 568, 982, 995, 1004, 1062, 1201
HpyF3I CTNAG 3 cut(s) 813, 1859, 1965
HpySE526I ACGT 3 cut(s) 300, 1873, 1961
HspAI GCGC 3 cut(s) 157, 284, 562
Kpn2I TCCGGA 1 cut(s) 580
Ksp22I TGATCA 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 1279
LguI GCTCTTC 2 cut(s) 1508, 1997
LmnI GCTCC 8 cut(s) 129, 396, 438, 937, 1125, 1186, 1409, 1498
Lsp1109I GCAGC 6 cut(s) 133, 888, 916, 1010, 1160, 1799
LweI GCATC 7 cut(s) 1052, 1107, 1191, 1294, 1468, 1476, 1879
MabI ACCWGGT 1 cut(s) 1864
MaeI CTAG 3 cut(s) 242, 1181, 1295
MaeII ACGT 3 cut(s) 300, 1873, 1961
MaeIII GTNAC 6 cut(s) 76, 431, 482, 839, 1082, 1417
MbiI CCGCTC 3 cut(s) 399, 441, 571
MboII GAAGA 8 cut(s) 76, 359, 1184, 1495, 1579, 1789, 1822, 1984
MfeI CAATTG 1 cut(s) 927
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 4 cut(s) 118, 638, 987, 1503
MlsI TGGCCA 3 cut(s) 527, 649, 1429
MluCI AATT 9 cut(s) 391, 601, 625, 927, 962, 1072, 1536, 1648, 1948
MluNI TGGCCA 3 cut(s) 527, 649, 1429
MlyI GAGTC 2 cut(s) 1072, 1325
MmeI TCCRAC 2 cut(s) 276, 847
Mox20I TGGCCA 3 cut(s) 527, 649, 1429
Mph1103I ATGCAT 2 cut(s) 1382, 1734
MroI TCCGGA 1 cut(s) 580
MscI TGGCCA 3 cut(s) 527, 649, 1429
MslI CAYNNNNRTG 1 cut(s) 920
Msp20I TGGCCA 3 cut(s) 527, 649, 1429
MspA1I CMGCKG 3 cut(s) 310, 907, 1790
MspI CCGG 6 cut(s) 108, 581, 588, 758, 1123, 1346
MspR9I CCNGG 5 cut(s) 759, 1124, 1346, 1654, 1866
MunI CAATTG 1 cut(s) 927
MvaI CCWGG 2 cut(s) 1654, 1866
MvnI CGCG 3 cut(s) 157, 284, 660
MwoI GCNNNNNNNGC 9 cut(s) 256, 283, 422, 568, 982, 995, 1004, 1062, 1201
NciI CCSGG 3 cut(s) 759, 1124, 1346
NcoI CCATGG 3 cut(s) 339, 644, 1932
NlaIV GGNNCC 8 cut(s) 35, 115, 116, 337, 612, 1671, 1684, 1971
NmuCI GTSAC 5 cut(s) 76, 431, 839, 1082, 1417
NsiI ATGCAT 2 cut(s) 1382, 1734
NspI RCATGY 3 cut(s) 1380, 1441, 1787
PaeI GCATGC 2 cut(s) 1380, 1441
PagI TCATGA 1 cut(s) 66
PciSI GCTCTTC 2 cut(s) 1508, 1997
PcsI WCGNNNNNNNCGW 4 cut(s) 18, 360, 366, 492
PfeI GAWTC 2 cut(s) 584, 1514
PflMI CCANNNNNTGG 2 cut(s) 645, 1938
PfoI TCCNGGA 1 cut(s) 757
PinAI ACCGGT 1 cut(s) 107
PleI GAGTC 2 cut(s) 1072, 1325
PpsI GAGTC 2 cut(s) 1072, 1325
PpuMI RGGWCCY 1 cut(s) 1970
PshBI ATTAAT 1 cut(s) 1071
PsiI TTATAA 1 cut(s) 1164
Psp124BI GAGCTC 1 cut(s) 1503
Psp5II RGGWCCY 1 cut(s) 1970
Psp6I CCWGG 2 cut(s) 1652, 1864
PspEI GGTNACC 1 cut(s) 839
PspFI CCCAGC 2 cut(s) 117, 1227
PspGI CCWGG 2 cut(s) 1652, 1864
PspN4I GGNNCC 8 cut(s) 35, 115, 116, 337, 612, 1671, 1684, 1971
PspOMI GGGCCC 1 cut(s) 114
PspPI GGNCC 6 cut(s) 105, 114, 115, 1682, 1849, 1970
PspPPI RGGWCCY 1 cut(s) 1970
PstI CTGCAG 1 cut(s) 906
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 1 cut(s) 1790
RsaI GTAC 5 cut(s) 500, 684, 797, 896, 1025
RsaNI GTAC 5 cut(s) 499, 683, 796, 895, 1024
RseI CAYNNNNRTG 1 cut(s) 920
SacI GAGCTC 1 cut(s) 1503
SapI GCTCTTC 2 cut(s) 1508, 1997
Sau96I GGNCC 6 cut(s) 105, 114, 115, 1682, 1849, 1970
ScaI AGTACT 1 cut(s) 797
SchI GAGTC 2 cut(s) 1072, 1325
ScrFI CCNGG 5 cut(s) 759, 1124, 1346, 1654, 1866
SduI GDGCHC 4 cut(s) 118, 638, 987, 1503
SexAI ACCWGGT 1 cut(s) 1864
SfaNI GCATC 7 cut(s) 1052, 1107, 1191, 1294, 1468, 1476, 1879
SfcI CTRYAG 2 cut(s) 902, 1984
SinI GGWCC 3 cut(s) 105, 1849, 1970
SmiMI CAYNNNNRTG 1 cut(s) 920
SmlI CTYRAG 4 cut(s) 971, 1103, 1333, 1841
SmoI CTYRAG 4 cut(s) 971, 1103, 1333, 1841
SphI GCATGC 2 cut(s) 1380, 1441
Sse9I AATT 9 cut(s) 391, 601, 625, 927, 962, 1072, 1536, 1648, 1948
SspI AATATT 1 cut(s) 1523
SspMI CTAG 3 cut(s) 242, 1181, 1295
SstI GAGCTC 1 cut(s) 1503
StyD4I CCNGG 5 cut(s) 757, 1122, 1344, 1652, 1864
StyI CCWWGG 3 cut(s) 339, 644, 1932
TaaI ACNGT 6 cut(s) 181, 488, 507, 774, 1712, 1853
TaiI ACGT 3 cut(s) 303, 1876, 1964
TaqI TCGA 2 cut(s) 354, 369
TasI AATT 9 cut(s) 391, 601, 625, 927, 962, 1072, 1536, 1648, 1948
TatI WGTACW 2 cut(s) 498, 795
TfiI GAWTC 2 cut(s) 584, 1514
TscAI CASTG 3 cut(s) 463, 1196, 1629
TseFI GTSAC 5 cut(s) 76, 431, 839, 1082, 1417
TseI GCWGC 6 cut(s) 121, 901, 904, 998, 1148, 1787
Tsp45I GTSAC 5 cut(s) 76, 431, 839, 1082, 1417
TspDTI ATGAA 4 cut(s) 1185, 1545, 1676, 1984
TspGWI ACGGA 3 cut(s) 64, 1541, 1874
TspRI CASTG 3 cut(s) 463, 1196, 1629
Van91I CCANNNNNTGG 2 cut(s) 645, 1938
VpaK11BI GGWCC 3 cut(s) 105, 1849, 1970
VspI ATTAAT 1 cut(s) 1071
XapI RAATTY 1 cut(s) 1648
XceI RCATGY 3 cut(s) 1380, 1441, 1787
XmiI GTMKAC 1 cut(s) 1878
XspI CTAG 3 cut(s) 242, 1181, 1295
ZrmI AGTACT 1 cut(s) 797
Zsp2I ATGCAT 2 cut(s) 1382, 1734
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.