pycom01g16000

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0011819
Physical Location & Seq
Reverse (-)
15726859 .. 15729064
2206 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g16000.1

Sequence Viewer

Length: 1296 bp
ATGACAATTTTGGGCAATGGCGATTGGCTGCGAATCCCGTTGATCCTGAAAGTTTGCGGATTTGTGATGATGGCAGTCACCTTCTTCTACTTCGGGAAGCACTGGTCCGATGGGTCCCAGCAGCTACTCTTCTTCACCACCACCACGAGCCGAGCACCCTCCGCCGTCTCCGTCTCGCCCAACCTAGACAAGCCTTTCAATCTCTCCGCCCTCATAAACGAAACCCTAGCTCCACCCCCGTTGCTTCCGTCTTCGCCGCCTCCGCCGCCGCCCGATGTGGTCCAGAGGTTCGGGATCGTCGACGAGAACGGCACCATGTCTGACGAGTTCGAGGTCGGGGATTTCGATCCCGATAAGGTGGTGGAGAATTGGGGCAGCCAGAACGGGAGTGTGGCGGAGAGTGAGCGGAGCGGGGAGGGTCGGGTGAGTGTGAAGAGGTTCGAGCTCTGCCCGAAGAGTATGAGCGAGTACATACCTTGTTTGGATAATGTGGAGGTGATTAAGAGGCTGAAATCGACGGCGAAAGGCGAGCGGTTCGAGCGGCATTGCCCGGAGGCTGGTCGGGGTTTGAATTGCTTGGTTCCGCCGCCCAAGAGCTACAAGACCCCAATTCCGTGGCCTAAAAGCCGGGATGAGGTTTGGTTCTTCAATGTACCTCATACAAAATTAGTGGAAGATAAAGGGGGTCAGAATTGGATTACCAGACAGAAGAACAAGTTTAAGTTTCCGGGAGGTGGTACACAGTTTATACATGGAGCAGATGGTTACTTGACTGATATTTCTAAGGCGGTCCCAGACATCACCTTCGGTCAGCATGTCCGTGTTGTTTTGGATGTCGGATGTGGTGTTGCAAGTTTTGGTGCGTATCTGCTGTCGCGAGATGTCCTTACTATGTCAGTTGCACCTAAAGACGTCCATGAGAACCAGATTCAATTTGCTCTTGAGCGCGGTGTGCCTGCAATGGTAGCAGCATTTGCAACTCACCGTTTATTGTACCCAAGTCAGGCATTTGACCTGATACATTGTTCACGATGTAGGATTAGCTGGACTCGTGACGATGGCATATTGTTGCTTGAGGTTAATAGGATGCTCAGGGCAGGAGGATACTTCGCTTGGGCAGCACAGCCAGTTTATAAGCACAAAGAAGTTCTAGAGGAGCAGTGGCAAGAGATGCTTAACCTCACCACGCGGCTTTGCTGGGAATTGGTGTCAAAGCAGGGCTATGTTGCAATATGGAAAAAGCCGGTTAACAACAGCTGCTATCTGAATCGGGATGAAGGAACCAAACCTCCATGA

Protein Analysis

432

Amino Acids

48.32

Weight (kDa)

7.56

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 156 - 431 2.7e-134 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25 PF13649 276 - 368 3.1e-06 Methyltransferase domain
Methyltransf_11 PF08241 277 - 371 8.7e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39750 AT5G06050
fragaria_vesca FvH4_7g23080 FvH4_7g23080
malus_domestica MD01G1139400.v1.1 MD07G1203800.v1.1
prunus_persica Prupe.2G239900_v2.0.a1
pyrus_communis pycom01g16000 pycom07g19040
rosa_chinensis RchiOBHm_Chr1g0368331
rosa_laevigata RLG00000027219
rosa_roxburghii Rroxscaffold_4G00288640
rosa_rugosa Rorug01G0341200 Rorug01G0341200
rosa_samantha Rh1AG347800 Rh1BG310800 Rh1CG326200 Rh1DG342600
rosa_wichuraiana Rw1G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1134
AatII GACGTC 1 cut(s) 915
AccB1I GGYRCC 1 cut(s) 311
AccBSI CCGCTC 4 cut(s) 406, 411, 532, 541
AccI GTMKAC 1 cut(s) 300
AccII CGCG 3 cut(s) 877, 948, 1189
AclWI GGATC 3 cut(s) 37, 302, 341
AcyI GRCGYC 1 cut(s) 912
AfaI GTAC 4 cut(s) 470, 654, 739, 995
AfiI CCNNNNNNNGG 4 cut(s) 557, 634, 734, 1003
AgsI TTSAA 4 cut(s) 199, 571, 649, 932
AloI GAACNNNNNNTCC 1 cut(s) 1273
AluBI AGCT 6 cut(s) 124, 230, 445, 597, 1044, 1257
AluI AGCT 6 cut(s) 124, 230, 445, 597, 1044, 1257
Alw21I GWGCWC 2 cut(s) 157, 447
Alw26I GTCTC 2 cut(s) 172, 178
AlwI GGATC 3 cut(s) 37, 302, 341
AoxI GGCC 1 cut(s) 617
ApeKI GCWGC 6 cut(s) 28, 121, 375, 968, 1118, 1257
ArsI GACNNNNNNTTYG 2 cut(s) 788, 820
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 1 cut(s) 948
AspS9I GGNCC 4 cut(s) 105, 114, 280, 790
AsuC2I CCSGG 3 cut(s) 551, 629, 729
AsuHPI GGTGA 7 cut(s) 70, 127, 436, 508, 793, 974, 1174
AvaII GGWCC 4 cut(s) 105, 114, 280, 790
BanI GGYRCC 1 cut(s) 311
BanII GRGCYC 1 cut(s) 447
BauI CACGAG 2 cut(s) 145, 1050
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 2 cut(s) 157, 447
BbvI GCAGC 6 cut(s) 15, 133, 387, 980, 1130, 1244
BccI CCATC 4 cut(s) 64, 104, 755, 1052
BceAI ACGGC 3 cut(s) 149, 325, 534
BciVI GTATCC 1 cut(s) 1097
BcnI CCSGG 3 cut(s) 551, 629, 729
BcoDI GTCTC 2 cut(s) 172, 178
BfaI CTAG 3 cut(s) 185, 227, 1151
BfuI GTATCC 1 cut(s) 1097
Bme1390I CCNGG 3 cut(s) 551, 629, 729
Bme18I GGWCC 4 cut(s) 105, 114, 280, 790
BmgT120I GGNCC 4 cut(s) 105, 114, 280, 790
BmiI GGNNCC 6 cut(s) 115, 116, 313, 582, 792, 1282
BmrFI CCNGG 3 cut(s) 551, 629, 729
BmsI GCATC 2 cut(s) 1077, 1161
BpiI GAAGAC 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 1091
BpuEI CTTGAG 2 cut(s) 962, 1094
BpuMI CCSGG 3 cut(s) 551, 629, 729
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 912
BsaJI CCNNGG 1 cut(s) 614
BsaXI ACNNNNNCTCC 3 cut(s) 214, 244, 1273
Bsc4I CCNNNNNNNGG 4 cut(s) 557, 634, 734, 1003
Bse118I RCCGGY 1 cut(s) 1243
Bse1I ACTGG 2 cut(s) 107, 1127
Bse3DI GCAATG 3 cut(s) 22, 544, 966
Bse8I GATNNNNATC 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 614
BseGI GGATG 5 cut(s) 637, 838, 845, 1092, 1279
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 4 cut(s) 557, 634, 734, 1003
BseMI GCAATG 3 cut(s) 22, 544, 966
BseMII CTCAG 1 cut(s) 1105
BseNI ACTGG 2 cut(s) 107, 1127
BseRI GAGGAG 1 cut(s) 1169
BseXI GCAGC 6 cut(s) 15, 133, 387, 980, 1130, 1244
BseYI CCCAGC 2 cut(s) 117, 1197
Bsh1236I CGCG 3 cut(s) 877, 948, 1189
BshFI GGCC 1 cut(s) 619
BshNI GGYRCC 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 157, 447
BsiSI CCGG 4 cut(s) 551, 628, 728, 1244
BslFI GGGAC 2 cut(s) 100, 776
BslI CCNNNNNNNGG 4 cut(s) 557, 634, 734, 1003
BsmAI GTCTC 2 cut(s) 172, 178
BsmBI CGTCTC 2 cut(s) 172, 178
BsmFI GGGAC 2 cut(s) 100, 776
BsnI GGCC 1 cut(s) 619
Bsp1286I GDGCHC 2 cut(s) 157, 447
Bsp143I GATC 3 cut(s) 42, 294, 346
Bsp68I TCGCGA 1 cut(s) 877
BspANI GGCC 1 cut(s) 619
BspCNI CTCAG 1 cut(s) 1104
BspFNI CGCG 3 cut(s) 877, 948, 1189
BspLI GGNNCC 6 cut(s) 115, 116, 313, 582, 792, 1282
BspPI GGATC 3 cut(s) 37, 302, 341
BspT107I GGYRCC 1 cut(s) 311
BsrBI CCGCTC 4 cut(s) 406, 411, 532, 541
BsrDI GCAATG 3 cut(s) 22, 544, 966
BsrFI RCCGGY 1 cut(s) 1243
BsrI ACTGG 2 cut(s) 107, 1127
BssAI RCCGGY 1 cut(s) 1243
BssECI CCNNGG 1 cut(s) 614
BssMI GATC 3 cut(s) 42, 294, 346
BssNI GRCGYC 1 cut(s) 912
BssSI CACGAG 2 cut(s) 145, 1050
Bst2BI CACGAG 2 cut(s) 145, 1050
Bst4CI ACNGT 2 cut(s) 744, 986
Bst6I CTCTTC 3 cut(s) 134, 428, 449
BstACI GRCGYC 1 cut(s) 912
BstAPI GCANNNNNTGC 2 cut(s) 974, 1171
BstC8I GCNNGC 2 cut(s) 530, 957
BstDEI CTNAG 2 cut(s) 783, 1091
BstDSI CCRYGG 1 cut(s) 614
BstF5I GGATG 5 cut(s) 637, 838, 845, 1092, 1279
BstFNI CGCG 3 cut(s) 877, 948, 1189
BstHHI GCGC 1 cut(s) 948
BstKTI GATC 3 cut(s) 45, 297, 349
BstMAI GTCTC 2 cut(s) 172, 178
BstMBI GATC 3 cut(s) 42, 294, 346
BstMWI GCNNNNNNNGC 9 cut(s) 161, 262, 265, 538, 952, 965, 974, 1118, 1171
BstNSI RCATGY 1 cut(s) 818
BstSCI CCNGG 3 cut(s) 549, 627, 727
BstUI CGCG 3 cut(s) 877, 948, 1189
BstV1I GCAGC 6 cut(s) 15, 133, 387, 980, 1130, 1244
BstV2I GAAGAC 1 cut(s) 243
BstXI CCANNNNNNTGG 1 cut(s) 615
BsuI GTATCC 1 cut(s) 1097
BsuRI GGCC 1 cut(s) 619
BtgI CCRYGG 1 cut(s) 614
BtsCI GGATG 5 cut(s) 637, 838, 845, 1092, 1279
BtsI GCAGTG 1 cut(s) 1166
BtsIMutI CAGTG 2 cut(s) 100, 1166
BtuMI TCGCGA 1 cut(s) 877
Cac8I GCNNGC 2 cut(s) 530, 957
CfoI GCGC 1 cut(s) 948
Cfr10I RCCGGY 1 cut(s) 1243
Cfr13I GGNCC 4 cut(s) 105, 114, 280, 790
Csp6I GTAC 4 cut(s) 469, 653, 738, 994
CspCI CAANNNNNGTGG 4 cut(s) 222, 257, 651, 686
CviAII CATG 5 cut(s) 316, 752, 815, 917, 1293
CviQI GTAC 4 cut(s) 469, 653, 738, 994
DdeI CTNAG 2 cut(s) 783, 1091
DpnI GATC 3 cut(s) 44, 296, 348
DpnII GATC 3 cut(s) 42, 294, 346
Eam1104I CTCTTC 3 cut(s) 134, 428, 449
EarI CTCTTC 3 cut(s) 134, 428, 449
EciI GGCGGA 5 cut(s) 151, 196, 252, 410, 573
Ecl136II GAGCTC 1 cut(s) 445
Eco24I GRGCYC 1 cut(s) 447
Eco47I GGWCC 4 cut(s) 105, 114, 280, 790
Eco53kI GAGCTC 1 cut(s) 445
EcoICRI GAGCTC 1 cut(s) 445
EcoO109I RGGNCCY 1 cut(s) 114
EcoT38I GRGCYC 1 cut(s) 447
Esp3I CGTCTC 2 cut(s) 172, 178
FaeI CATG 5 cut(s) 319, 755, 818, 920, 1296
FalI AAGNNNNNCTT 2 cut(s) 1158, 1190
FaqI GGGAC 2 cut(s) 100, 776
FatI CATG 5 cut(s) 315, 751, 814, 916, 1292
FauI CCCGC 1 cut(s) 404
FblI GTMKAC 1 cut(s) 300
FokI GGATG 5 cut(s) 644, 845, 852, 1099, 1286
FriOI GRGCYC 1 cut(s) 447
FspBI CTAG 3 cut(s) 185, 227, 1151
GlaI GCGC 1 cut(s) 947
GsaI CCCAGC 2 cut(s) 121, 1201
HaeIII GGCC 1 cut(s) 619
HapII CCGG 4 cut(s) 551, 628, 728, 1244
HhaI GCGC 1 cut(s) 948
Hin1I GRCGYC 1 cut(s) 912
Hin1II CATG 5 cut(s) 319, 755, 818, 920, 1296
Hin6I GCGC 1 cut(s) 946
HinP1I GCGC 1 cut(s) 946
HincII GTYRAC 2 cut(s) 301, 1249
HindII GTYRAC 2 cut(s) 301, 1249
HinfI GANTC 4 cut(s) 33, 928, 1048, 1267
HpaI GTTAAC 1 cut(s) 1249
HpaII CCGG 4 cut(s) 551, 628, 728, 1244
HphI GGTGA 7 cut(s) 70, 127, 436, 508, 793, 974, 1174
Hpy166II GTNNAC 4 cut(s) 301, 740, 1028, 1249
Hpy188I TCNGA 5 cut(s) 109, 322, 690, 839, 1266
Hpy8I GTNNAC 4 cut(s) 301, 740, 1028, 1249
Hpy99I CGWCG 3 cut(s) 302, 305, 520
HpyAV CCTTC 3 cut(s) 91, 814, 1271
HpyCH4III ACNGT 2 cut(s) 744, 986
HpyCH4IV ACGT 1 cut(s) 912
HpyCH4V TGCA 5 cut(s) 851, 902, 959, 977, 1229
HpyF10VI GCNNNNNNNGC 9 cut(s) 161, 262, 265, 538, 952, 965, 974, 1118, 1171
HpyF3I CTNAG 2 cut(s) 783, 1091
HpySE526I ACGT 1 cut(s) 912
Hsp92I GRCGYC 1 cut(s) 912
Hsp92II CATG 5 cut(s) 319, 755, 818, 920, 1296
HspAI GCGC 1 cut(s) 946
KflI GGGWCCC 1 cut(s) 114
KspAI GTTAAC 1 cut(s) 1249
Kzo9I GATC 3 cut(s) 42, 294, 346
LmnI GCTCC 4 cut(s) 235, 408, 755, 1156
Lsp1109I GCAGC 6 cut(s) 15, 133, 387, 980, 1130, 1244
LweI GCATC 2 cut(s) 1077, 1161
MaeI CTAG 3 cut(s) 185, 227, 1151
MaeII ACGT 1 cut(s) 912
MaeIII GTNAC 3 cut(s) 76, 764, 1052
MalI GATC 3 cut(s) 44, 296, 348
MbiI CCGCTC 4 cut(s) 406, 411, 532, 541
MboI GATC 3 cut(s) 42, 294, 346
MboII GAAGA 9 cut(s) 76, 121, 124, 243, 445, 466, 637, 686, 721
MhlI GDGCHC 2 cut(s) 157, 447
MluCI AATT 8 cut(s) 6, 367, 571, 609, 665, 691, 932, 1202
MlyI GAGTC 1 cut(s) 1042
MmeI TCCRAC 1 cut(s) 817
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 5 cut(s) 501, 720, 1080, 1176, 1248
MslI CAYNNNNRTG 1 cut(s) 819
MspA1I CMGCKG 1 cut(s) 1257
MspI CCGG 4 cut(s) 551, 628, 728, 1244
MspR9I CCNGG 3 cut(s) 551, 629, 729
MvnI CGCG 3 cut(s) 877, 948, 1189
MwoI GCNNNNNNNGC 9 cut(s) 161, 262, 265, 538, 952, 965, 974, 1118, 1171
NciI CCSGG 3 cut(s) 551, 629, 729
NdeII GATC 3 cut(s) 42, 294, 346
NlaIII CATG 5 cut(s) 319, 755, 818, 920, 1296
NlaIV GGNNCC 6 cut(s) 115, 116, 313, 582, 792, 1282
NmeAIII GCCGAG 1 cut(s) 176
NmuCI GTSAC 2 cut(s) 76, 1052
NruI TCGCGA 1 cut(s) 877
NspI RCATGY 1 cut(s) 818
PcsI WCGNNNNNNNCGW 2 cut(s) 297, 342
PdmI GAANNNNTTC 1 cut(s) 437
PfeI GAWTC 3 cut(s) 33, 928, 1267
PfoI TCCNGGA 1 cut(s) 727
PleI GAGTC 1 cut(s) 1042
PpsI GAGTC 1 cut(s) 1042
PpuMI RGGWCCY 1 cut(s) 114
PsiI TTATAA 1 cut(s) 1134
Psp124BI GAGCTC 1 cut(s) 447
Psp5II RGGWCCY 1 cut(s) 114
PspFI CCCAGC 2 cut(s) 117, 1197
PspN4I GGNNCC 6 cut(s) 115, 116, 313, 582, 792, 1282
PspPI GGNCC 4 cut(s) 105, 114, 280, 790
PspPPI RGGWCCY 1 cut(s) 114
PvuII CAGCTG 1 cut(s) 1257
RruI TCGCGA 1 cut(s) 877
RsaI GTAC 4 cut(s) 470, 654, 739, 995
RsaNI GTAC 4 cut(s) 469, 653, 738, 994
RseI CAYNNNNRTG 1 cut(s) 819
SacI GAGCTC 1 cut(s) 447
SalI GTCGAC 1 cut(s) 299
SaqAI TTAA 5 cut(s) 501, 720, 1080, 1176, 1248
Sau3AI GATC 3 cut(s) 42, 294, 346
Sau96I GGNCC 4 cut(s) 105, 114, 280, 790
SchI GAGTC 1 cut(s) 1042
ScrFI CCNGG 3 cut(s) 551, 629, 729
SduI GDGCHC 2 cut(s) 157, 447
SfaNI GCATC 2 cut(s) 1077, 1161
SgrDI CGTCGACG 1 cut(s) 299
SinI GGWCC 4 cut(s) 105, 114, 280, 790
SmiMI CAYNNNNRTG 1 cut(s) 819
SmlI CTYRAG 2 cut(s) 941, 1073
SmoI CTYRAG 2 cut(s) 941, 1073
Sse9I AATT 8 cut(s) 6, 367, 571, 609, 665, 691, 932, 1202
SspMI CTAG 3 cut(s) 185, 227, 1151
SstI GAGCTC 1 cut(s) 447
StyD4I CCNGG 3 cut(s) 549, 627, 727
TaaI ACNGT 2 cut(s) 744, 986
TaiI ACGT 1 cut(s) 915
TaqI TCGA 6 cut(s) 300, 330, 345, 441, 515, 537
TaqII GACCGA 1 cut(s) 797
TasI AATT 8 cut(s) 6, 367, 571, 609, 665, 691, 932, 1202
TatI WGTACW 1 cut(s) 468
TauI GCSGC 6 cut(s) 259, 268, 271, 544, 589, 1192
TfiI GAWTC 3 cut(s) 33, 928, 1267
Tru1I TTAA 5 cut(s) 501, 720, 1080, 1176, 1248
Tru9I TTAA 5 cut(s) 501, 720, 1080, 1176, 1248
TscAI CASTG 2 cut(s) 107, 1166
TseFI GTSAC 2 cut(s) 76, 1052
TseI GCWGC 6 cut(s) 28, 121, 375, 968, 1118, 1257
Tsp45I GTSAC 2 cut(s) 76, 1052
TspDTI ATGAA 1 cut(s) 1290
TspGWI ACGGA 4 cut(s) 160, 237, 603, 809
TspRI CASTG 2 cut(s) 107, 1166
VpaK11BI GGWCC 4 cut(s) 105, 114, 280, 790
XbaI TCTAGA 1 cut(s) 1150
XceI RCATGY 1 cut(s) 818
XmiI GTMKAC 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 3 cut(s) 185, 227, 1151
ZraI GACGTC 1 cut(s) 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.