RLG00000027219

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0011819
Physical Location & Seq
Forward (+)
9676621 .. 9681352
4732 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027219

Sequence Viewer

Length: 2040 bp
ATGGGATTTTTCGGGAATGGCGATTGGTTGAGGATCCCATTGATCATCAAAATATGGGGGTTTATCATGATCTCCGTCACCTTCTTCTACCTGGGCAAGAACTGGACCGGTGGGGCCCAGCAGCTCCTCTCCTTCACCACCGCCGCCACGCGCCGATCTCCTCCTTCCGCCACGGTCTCCATTTCCCCCAATCTCAACAAGCCTTTCAACCTCTCCGCCCTCCTCAACGAAACCCTAGCCCCGCCGCCGCTGCCTTCTCCGCCTCCGCCTCCGCCGCCGACTCCATCTCCTCCGCCCCCCGCCCCGCCGCCGTCGGACGTTATCCAGCGGTTCGGGATCGTGGACGAGAACGGGACCATGGCCGACGATTTCGAGGTCGGCGAGTTCGATCCCGATGAGGTGGTGGAGAATTGGAGCGGCCAGAATGGGACGGCGGTGGCGGAGAGTGACGGGAGCGGGTCGGGTAGGTTCAGTGGGAAAAGGTTTGAGCTTTGTCGTAACGGTATGAGCGAGTACATACCGTGTTTGGATAATGTGGTGGCCATTAAGAGGCTCAAATCCACCGATAAAGGCGAGCGCTTTGAGCGGCATTGTCCGGATTCCGGCAAGGGTTTGAATTGCTTGGTTCCGCCGCCCAAGAATTACAAGAGCCCCATTCCATGGCCGAAGAGCCGCGATGAGGTTTGGTTCTACAATGTACCTCATACTCGTCTGGTTGAGGATAAAGGGGGTCAGAACTGGATTACACGAGAAAAGAATAAGTTTAAGTTTCCGGGAGGTGGAACACAGTTTATACATGGGGCAAATGAGTACTTGGATCATATTTCTAAGATGATCCCTGACATCACTTTTGGTCACCATATTCGAGTTGCATTGGATGTTGGATGTGGTGTTGCAAGTTTTGGTGCATACCTGCTGCAACGGAATGTCATCACTATGTCAATTGCTCCGAAAGATGTCCATGAGAATCAGATACAATTTGCTCTTGAGCGTGGAGTGCCTGCAATGGTAGCAGCATTTGCTACTCGCCGTTTATTGTACCCAAGTCAGGCATTTGACTTGATCCATTGCTCACGATGCAGGATTAATTGGACTCGTGACGATGGGATATTGCTACTTGAGGTTAATAGGATGCTCCGGGCAGGAGGATACTTTGTTTGGGCAGCACAGCCAGTTTATAAGCATGAAGAAGTCCTAGAGGAGCAGTGGCAAGAGATGCTTAACCTCACGACCCGTCTTTGCTGGGAGTTTGTGAAAAAGGATGGCTATGTTGCCGTATGGCAGAAGCCAGTTAACAACAGCTGCTATCTAGGTCGGGATGCTGAAACCAAACCTCCATTATGCGACTCAAGTGATGACCCGGACAAAGTTTGGTATGTTGATCTGAAGGCGTGCATAACAAGACTCCCTGAAAATGGTTATGGAGCAAATGTCACAACATGGCCTGACCGCATGCAAACTCCCCCTGATAGGCTTCAGAGCATACAGTTTGATGCCTATATATCCAGAAAGGAGCTCTTCAAGGCAGAATCTAAATATTGGCATGAAATAATTGAAGGCTATGTCCGTGCTTTTAAATGGAAGAAGTTAAGATTAAGAAATGTAATGGATATGAGAGCTGGCTTTGGAGGATTTGCTGTGGCACTAATGGAACATAACTTAAATTCCTGGGTTATGAATGTGGTTCCTGTTAGCGGCCCCAACACCTTGCCTGTTATATATGACCGTGGATTTATTGGAGTTATGCATGACTGGTGTGAACCATTTGATACATACCCAAGAACCTATGATTTATTACATGCAGCTGGCCTCTTCTCTATTGAGAAGAAAAGGTGCAATATGTCAACAATCATGCTTGAGATGGACCGTATGCTAAGACCAGGTGGACGTGTATACATCCGTGATGCTCTTGCAGTCATGGATGAGCTTCAAGCGATTGGAAATGCCATGGGCTGGAGAGTTAATTTGCGAGACACATCTGAGGGTCCTCATGCAAGCTATAGGCTCTTCATGGCTGAAAAACGCCTCTTGCATAGTTAA

Protein Analysis

680

Amino Acids

76.87

Weight (kDa)

7.56

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 171 - 674 1.7e-231 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 292 - 386 7.6e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39750 AT5G06050
fragaria_vesca FvH4_7g23080 FvH4_7g23080
malus_domestica MD01G1139400.v1.1 MD07G1203800.v1.1
prunus_persica Prupe.2G239900_v2.0.a1
pyrus_communis pycom01g16000 pycom07g19040
rosa_chinensis RchiOBHm_Chr1g0368331
rosa_laevigata RLG00000027219
rosa_roxburghii Rroxscaffold_4G00288640
rosa_rugosa Rorug01G0341200 Rorug01G0341200
rosa_samantha Rh1AG347800 Rh1BG310800 Rh1CG326200 Rh1DG342600
rosa_wichuraiana Rw1G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1179
Acc36I ACCTGC 1 cut(s) 921
AccB7I CCANNNNNTGG 2 cut(s) 660, 1953
AccBSI CCGCTC 3 cut(s) 417, 456, 586
AccI GTMKAC 1 cut(s) 1893
AccII CGCG 2 cut(s) 151, 675
AccIII TCCGGA 1 cut(s) 595
AclWI GGATC 7 cut(s) 28, 41, 344, 383, 825, 829, 1057
AcoI YGGCCR 4 cut(s) 360, 418, 540, 662
AcsI RAATTY 1 cut(s) 1663
AcuI CTGAAG 2 cut(s) 1406, 1460
AfaI GTAC 4 cut(s) 515, 699, 812, 1040
AfeI AGCGCT 1 cut(s) 578
AflIII ACRYGT 1 cut(s) 1888
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 5 cut(s) 208, 616, 1522, 1556, 1931
AjiI CACGTC 1 cut(s) 1889
AjnI CCWGG 3 cut(s) 90, 1667, 1879
AluBI AGCT 8 cut(s) 124, 490, 1302, 1516, 1619, 1805, 1927, 1998
AluI AGCT 8 cut(s) 124, 490, 1302, 1516, 1619, 1805, 1927, 1998
Alw21I GWGCWC 1 cut(s) 1518
Alw26I GTCTC 2 cut(s) 181, 1965
AlwI GGATC 7 cut(s) 28, 41, 344, 383, 825, 829, 1057
Aor13HI TCCGGA 1 cut(s) 595
Aor51HI AGCGCT 1 cut(s) 578
AoxI GGCC 8 cut(s) 114, 360, 418, 540, 662, 1442, 1696, 1807
ApaI GGGCCC 1 cut(s) 118
ApeKI GCWGC 7 cut(s) 121, 250, 916, 1013, 1163, 1302, 1802
ApoI RAATTY 1 cut(s) 1663
ArsI GACNNNNNNTTYG 2 cut(s) 833, 865
AseI ATTAAT 1 cut(s) 1086
AsiGI ACCGGT 1 cut(s) 107
AspLEI GCGC 2 cut(s) 153, 579
AspS9I GGNCC 7 cut(s) 105, 114, 115, 354, 1697, 1864, 1985
AsuC2I CCSGG 3 cut(s) 774, 1139, 1361
AsuHPI GGTGA 3 cut(s) 70, 127, 848
AvaII GGWCC 4 cut(s) 105, 354, 1864, 1985
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 1 cut(s) 542
BamHI GGATCC 1 cut(s) 33
BanII GRGCYC 3 cut(s) 118, 653, 1518
BauI CACGAG 2 cut(s) 747, 1095
Bbv12I GWGCWC 1 cut(s) 1518
BbvI GCAGC 7 cut(s) 133, 237, 903, 1025, 1175, 1289, 1814
BccI CCATC 4 cut(s) 292, 1097, 1256, 1855
BceAI ACGGC 4 cut(s) 295, 447, 1014, 1259
BciT130I CCWGG 3 cut(s) 92, 1669, 1881
BciVI GTATCC 1 cut(s) 1142
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 3 cut(s) 774, 1139, 1361
BcoDI GTCTC 2 cut(s) 181, 1965
BfaI CTAG 3 cut(s) 236, 1196, 1310
BfmI CTRYAG 1 cut(s) 1999
BfoI RGCGCY 1 cut(s) 580
BfuAI ACCTGC 1 cut(s) 921
BfuI GTATCC 1 cut(s) 1142
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 6 cut(s) 92, 774, 1139, 1361, 1669, 1881
Bme18I GGWCC 4 cut(s) 105, 354, 1864, 1985
BmgBI CACGTC 1 cut(s) 1889
BmgT120I GGNCC 7 cut(s) 105, 114, 115, 354, 1697, 1864, 1985
BmiI GGNNCC 8 cut(s) 35, 115, 116, 355, 627, 1686, 1699, 1986
BmrFI CCNGG 6 cut(s) 92, 774, 1139, 1361, 1669, 1881
BmsI GCATC 6 cut(s) 1067, 1122, 1206, 1309, 1483, 1894
BpmI CTGGAG 1 cut(s) 1975
BpuEI CTTGAG 4 cut(s) 1007, 1139, 1333, 1877
BpuMI CCSGG 3 cut(s) 774, 1139, 1361
BsaI GGTCTC 1 cut(s) 181
BsaJI CCNNGG 7 cut(s) 91, 171, 357, 659, 1668, 1726, 1947
BsaWI WCCGGW 2 cut(s) 107, 595
BsaXI ACNNNNNCTCC 6 cut(s) 271, 301, 1318, 1348, 1416, 1446
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 5 cut(s) 107, 743, 1172, 1289, 1757
Bse3DI GCAATG 2 cut(s) 1011, 1066
BseAI TCCGGA 1 cut(s) 595
BseBI CCWGG 3 cut(s) 92, 1669, 1881
BseDI CCNNGG 7 cut(s) 91, 171, 357, 659, 1668, 1726, 1947
BseGI GGATG 7 cut(s) 883, 890, 1137, 1267, 1324, 1896, 1927
BseMI GCAATG 2 cut(s) 1011, 1066
BseMII CTCAG 1 cut(s) 1971
BseNI ACTGG 5 cut(s) 107, 743, 1172, 1289, 1757
BseRI GAGGAG 5 cut(s) 116, 150, 212, 279, 1214
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 7 cut(s) 133, 237, 903, 1025, 1175, 1289, 1814
BseYI CCCAGC 2 cut(s) 117, 1242
Bsh1236I CGCG 2 cut(s) 151, 675
BshFI GGCC 8 cut(s) 116, 362, 420, 542, 664, 1444, 1698, 1809
BshTI ACCGGT 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 1518
BsiSI CCGG 6 cut(s) 108, 596, 603, 773, 1138, 1361
BslFI GGGAC 2 cut(s) 367, 442
BsmAI GTCTC 2 cut(s) 181, 1965
BsmFI GGGAC 2 cut(s) 367, 442
BsnI GGCC 8 cut(s) 116, 362, 420, 542, 664, 1444, 1698, 1809
Bso31I GGTCTC 1 cut(s) 181
Bsp120I GGGCCC 1 cut(s) 114
Bsp1286I GDGCHC 3 cut(s) 118, 653, 1518
Bsp13I TCCGGA 1 cut(s) 595
Bsp19I CCATGG 3 cut(s) 357, 659, 1947
BspANI GGCC 8 cut(s) 116, 362, 420, 542, 664, 1444, 1698, 1809
BspCNI CTCAG 1 cut(s) 1972
BspEI TCCGGA 1 cut(s) 595
BspFNI CGCG 2 cut(s) 151, 675
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 8 cut(s) 35, 115, 116, 355, 627, 1686, 1699, 1986
BspMI ACCTGC 1 cut(s) 921
BspPI GGATC 7 cut(s) 28, 41, 344, 383, 825, 829, 1057
BspQI GCTCTTC 3 cut(s) 662, 1523, 2012
BspTNI GGTCTC 1 cut(s) 181
BsrBI CCGCTC 3 cut(s) 417, 456, 586
BsrDI GCAATG 2 cut(s) 1011, 1066
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 5 cut(s) 107, 743, 1172, 1289, 1757
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 7 cut(s) 91, 171, 357, 659, 1668, 1726, 1947
BssNAI GTATAC 1 cut(s) 1894
BssSI CACGAG 2 cut(s) 747, 1095
BssT1I CCWWGG 3 cut(s) 357, 659, 1947
Bst1107I GTATAC 1 cut(s) 1894
Bst2BI CACGAG 2 cut(s) 747, 1095
Bst2UI CCWGG 3 cut(s) 92, 1669, 1881
Bst4CI ACNGT 7 cut(s) 175, 503, 522, 789, 1488, 1727, 1868
Bst6I CTCTTC 4 cut(s) 662, 1523, 1817, 2012
BstAPI GCANNNNNTGC 2 cut(s) 1019, 1216
BstC8I GCNNGC 7 cut(s) 575, 1002, 1393, 1454, 1621, 1807, 1996
BstDEI CTNAG 3 cut(s) 828, 1874, 1980
BstDSI CCRYGG 5 cut(s) 171, 357, 659, 1726, 1947
BstEII GGTNACC 1 cut(s) 854
BstF5I GGATG 7 cut(s) 883, 890, 1137, 1267, 1324, 1896, 1927
BstFNI CGCG 2 cut(s) 151, 675
BstH2I RGCGCY 1 cut(s) 580
BstHHI GCGC 2 cut(s) 153, 579
BstMAI GTCTC 2 cut(s) 181, 1965
BstMWI GCNNNNNNNGC 9 cut(s) 250, 259, 274, 583, 997, 1010, 1019, 1077, 1216
BstNI CCWGG 3 cut(s) 92, 1669, 1881
BstNSI RCATGY 2 cut(s) 1456, 1802
BstPI GGTNACC 1 cut(s) 854
BstSCI CCNGG 6 cut(s) 90, 772, 1137, 1359, 1667, 1879
BstSFI CTRYAG 1 cut(s) 1999
BstSLI GKGCMC 1 cut(s) 118
BstUI CGCG 2 cut(s) 151, 675
BstV1I GCAGC 7 cut(s) 133, 237, 903, 1025, 1175, 1289, 1814
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BstZ17I GTATAC 1 cut(s) 1894
BsuI GTATCC 1 cut(s) 1142
BsuRI GGCC 8 cut(s) 116, 362, 420, 542, 664, 1444, 1698, 1809
BtgI CCRYGG 5 cut(s) 171, 357, 659, 1726, 1947
BtgZI GCGATG 1 cut(s) 690
BtrI CACGTC 1 cut(s) 1889
BtsCI GGATG 7 cut(s) 883, 890, 1137, 1267, 1324, 1896, 1927
BtsI GCAGTG 1 cut(s) 1211
BtsIMutI CAGTG 2 cut(s) 478, 1211
BveI ACCTGC 1 cut(s) 921
Cac8I GCNNGC 7 cut(s) 575, 1002, 1393, 1454, 1621, 1807, 1996
CciI TCATGA 1 cut(s) 66
CfoI GCGC 2 cut(s) 153, 579
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 7 cut(s) 105, 114, 115, 354, 1697, 1864, 1985
CsiI ACCWGGT 1 cut(s) 1879
Csp6I GTAC 4 cut(s) 514, 698, 811, 1039
CspAI ACCGGT 1 cut(s) 107
CviQI GTAC 4 cut(s) 514, 698, 811, 1039
DdeI CTNAG 3 cut(s) 828, 1874, 1980
DraI TTTAAA 1 cut(s) 1576
EaeI YGGCCR 4 cut(s) 360, 418, 540, 662
Eam1104I CTCTTC 4 cut(s) 662, 1523, 1817, 2012
EarI CTCTTC 4 cut(s) 662, 1523, 1817, 2012
EciI GGCGGA 8 cut(s) 157, 205, 249, 255, 261, 282, 455, 618
Ecl136II GAGCTC 1 cut(s) 1516
Eco130I CCWWGG 3 cut(s) 357, 659, 1947
Eco24I GRGCYC 3 cut(s) 118, 653, 1518
Eco31I GGTCTC 1 cut(s) 181
Eco47I GGWCC 4 cut(s) 105, 354, 1864, 1985
Eco47III AGCGCT 1 cut(s) 578
Eco53kI GAGCTC 1 cut(s) 1516
Eco57I CTGAAG 2 cut(s) 1406, 1460
Eco91I GGTNACC 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 1516
EcoO109I RGGNCCY 2 cut(s) 114, 1985
EcoO65I GGTNACC 1 cut(s) 854
EcoRII CCWGG 3 cut(s) 90, 1667, 1879
EcoT14I CCWWGG 3 cut(s) 357, 659, 1947
EcoT22I ATGCAT 1 cut(s) 1749
EcoT38I GRGCYC 3 cut(s) 118, 653, 1518
ErhI CCWWGG 3 cut(s) 357, 659, 1947
FalI AAGNNNNNCTT 4 cut(s) 1203, 1235, 1502, 1534
FaqI GGGAC 2 cut(s) 367, 442
FauI CCCGC 4 cut(s) 249, 307, 312, 449
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 1893
FokI GGATG 7 cut(s) 890, 897, 1144, 1274, 1331, 1883, 1934
FriOI GRGCYC 3 cut(s) 118, 653, 1518
FspBI CTAG 3 cut(s) 236, 1196, 1310
GlaI GCGC 2 cut(s) 152, 578
GsaI CCCAGC 2 cut(s) 121, 1246
GsuI CTGGAG 1 cut(s) 1975
HaeII RGCGCY 1 cut(s) 580
HaeIII GGCC 8 cut(s) 116, 362, 420, 542, 664, 1444, 1698, 1809
HapII CCGG 6 cut(s) 108, 596, 603, 773, 1138, 1361
HhaI GCGC 2 cut(s) 153, 579
Hin6I GCGC 2 cut(s) 151, 577
HinP1I GCGC 2 cut(s) 151, 577
HincII GTYRAC 2 cut(s) 1294, 1845
HindII GTYRAC 2 cut(s) 1294, 1845
HinfI GANTC 7 cut(s) 280, 599, 967, 1093, 1346, 1404, 1529
HpaI GTTAAC 1 cut(s) 1294
HpaII CCGG 6 cut(s) 108, 596, 603, 773, 1138, 1361
HphI GGTGA 3 cut(s) 70, 127, 848
Hpy166II GTNNAC 6 cut(s) 343, 1294, 1760, 1845, 1886, 1894
Hpy188I TCNGA 7 cut(s) 316, 735, 951, 972, 1386, 1479, 1981
Hpy8I GTNNAC 6 cut(s) 343, 1294, 1760, 1845, 1886, 1894
Hpy99I CGWCG 2 cut(s) 316, 368
HpyAV CCTTC 6 cut(s) 91, 142, 174, 264, 1381, 1550
HpyCH4III ACNGT 7 cut(s) 175, 503, 522, 789, 1488, 1727, 1868
HpyCH4IV ACGT 2 cut(s) 318, 1888
HpyF10VI GCNNNNNNNGC 9 cut(s) 250, 259, 274, 583, 997, 1010, 1019, 1077, 1216
HpyF3I CTNAG 3 cut(s) 828, 1874, 1980
HpySE526I ACGT 2 cut(s) 318, 1888
HspAI GCGC 2 cut(s) 151, 577
Kpn2I TCCGGA 1 cut(s) 595
Ksp22I TGATCA 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 1294
LguI GCTCTTC 3 cut(s) 662, 1523, 2012
LmnI GCTCC 8 cut(s) 129, 414, 453, 952, 1140, 1201, 1424, 1513
Lsp1109I GCAGC 7 cut(s) 133, 237, 903, 1025, 1175, 1289, 1814
LweI GCATC 6 cut(s) 1067, 1122, 1206, 1309, 1483, 1894
MabI ACCWGGT 1 cut(s) 1879
MaeI CTAG 3 cut(s) 236, 1196, 1310
MaeII ACGT 2 cut(s) 318, 1888
MaeIII GTNAC 6 cut(s) 76, 446, 497, 854, 1097, 1432
MbiI CCGCTC 3 cut(s) 417, 456, 586
MboII GAAGA 8 cut(s) 76, 679, 1199, 1510, 1594, 1804, 1837, 1999
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 3 cut(s) 118, 653, 1518
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 9 cut(s) 409, 616, 640, 942, 977, 1087, 1551, 1663, 1963
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 4 cut(s) 274, 1087, 1340, 1398
MmeI TCCRAC 2 cut(s) 294, 862
Mox20I TGGCCA 1 cut(s) 542
Mph1103I ATGCAT 1 cut(s) 1749
MroI TCCGGA 1 cut(s) 595
MscI TGGCCA 1 cut(s) 542
MslI CAYNNNNRTG 1 cut(s) 935
Msp20I TGGCCA 1 cut(s) 542
MspA1I CMGCKG 4 cut(s) 250, 328, 1302, 1805
MspI CCGG 6 cut(s) 108, 596, 603, 773, 1138, 1361
MspR9I CCNGG 6 cut(s) 92, 774, 1139, 1361, 1669, 1881
MunI CAATTG 1 cut(s) 942
MvaI CCWGG 3 cut(s) 92, 1669, 1881
MvnI CGCG 2 cut(s) 151, 675
MwoI GCNNNNNNNGC 9 cut(s) 250, 259, 274, 583, 997, 1010, 1019, 1077, 1216
NciI CCSGG 3 cut(s) 774, 1139, 1361
NcoI CCATGG 3 cut(s) 357, 659, 1947
NlaIV GGNNCC 8 cut(s) 35, 115, 116, 355, 627, 1686, 1699, 1986
NmuCI GTSAC 5 cut(s) 76, 446, 854, 1097, 1432
NsiI ATGCAT 1 cut(s) 1749
NspI RCATGY 2 cut(s) 1456, 1802
PaeI GCATGC 1 cut(s) 1456
PagI TCATGA 1 cut(s) 66
PciSI GCTCTTC 3 cut(s) 662, 1523, 2012
PcsI WCGNNNNNNNCGW 4 cut(s) 18, 378, 384, 507
PfeI GAWTC 3 cut(s) 599, 967, 1529
PflMI CCANNNNNTGG 2 cut(s) 660, 1953
PfoI TCCNGGA 1 cut(s) 772
PinAI ACCGGT 1 cut(s) 107
PleI GAGTC 4 cut(s) 274, 1087, 1340, 1398
PpsI GAGTC 4 cut(s) 274, 1087, 1340, 1398
PpuMI RGGWCCY 1 cut(s) 1985
PshBI ATTAAT 1 cut(s) 1086
PsiI TTATAA 1 cut(s) 1179
Psp124BI GAGCTC 1 cut(s) 1518
Psp5II RGGWCCY 1 cut(s) 1985
Psp6I CCWGG 3 cut(s) 90, 1667, 1879
PspEI GGTNACC 1 cut(s) 854
PspFI CCCAGC 2 cut(s) 117, 1242
PspGI CCWGG 3 cut(s) 90, 1667, 1879
PspN4I GGNNCC 8 cut(s) 35, 115, 116, 355, 627, 1686, 1699, 1986
PspOMI GGGCCC 1 cut(s) 114
PspPI GGNCC 7 cut(s) 105, 114, 115, 354, 1697, 1864, 1985
PspPPI RGGWCCY 1 cut(s) 1985
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 2 cut(s) 1302, 1805
RsaI GTAC 4 cut(s) 515, 699, 812, 1040
RsaNI GTAC 4 cut(s) 514, 698, 811, 1039
RseI CAYNNNNRTG 1 cut(s) 935
SacI GAGCTC 1 cut(s) 1518
SapI GCTCTTC 3 cut(s) 662, 1523, 2012
Sau96I GGNCC 7 cut(s) 105, 114, 115, 354, 1697, 1864, 1985
ScaI AGTACT 1 cut(s) 812
SchI GAGTC 4 cut(s) 274, 1087, 1340, 1398
ScrFI CCNGG 6 cut(s) 92, 774, 1139, 1361, 1669, 1881
SduI GDGCHC 3 cut(s) 118, 653, 1518
SexAI ACCWGGT 1 cut(s) 1879
SfaNI GCATC 6 cut(s) 1067, 1122, 1206, 1309, 1483, 1894
SfcI CTRYAG 1 cut(s) 1999
SinI GGWCC 4 cut(s) 105, 354, 1864, 1985
SmiMI CAYNNNNRTG 1 cut(s) 935
SmlI CTYRAG 4 cut(s) 986, 1118, 1348, 1856
SmoI CTYRAG 4 cut(s) 986, 1118, 1348, 1856
SphI GCATGC 1 cut(s) 1456
Sse9I AATT 9 cut(s) 409, 616, 640, 942, 977, 1087, 1551, 1663, 1963
SspI AATATT 1 cut(s) 1538
SspMI CTAG 3 cut(s) 236, 1196, 1310
SstI GAGCTC 1 cut(s) 1518
StyD4I CCNGG 6 cut(s) 90, 772, 1137, 1359, 1667, 1879
StyI CCWWGG 3 cut(s) 357, 659, 1947
TaaI ACNGT 7 cut(s) 175, 503, 522, 789, 1488, 1727, 1868
TaiI ACGT 2 cut(s) 321, 1891
TaqI TCGA 3 cut(s) 372, 387, 865
TasI AATT 9 cut(s) 409, 616, 640, 942, 977, 1087, 1551, 1663, 1963
TatI WGTACW 2 cut(s) 513, 810
TfiI GAWTC 3 cut(s) 599, 967, 1529
TscAI CASTG 2 cut(s) 478, 1211
TseFI GTSAC 5 cut(s) 76, 446, 854, 1097, 1432
TseI GCWGC 7 cut(s) 121, 250, 916, 1013, 1163, 1302, 1802
Tsp45I GTSAC 5 cut(s) 76, 446, 854, 1097, 1432
TspDTI ATGAA 4 cut(s) 1200, 1560, 1691, 1999
TspGWI ACGGA 4 cut(s) 64, 937, 1556, 1889
TspRI CASTG 2 cut(s) 478, 1211
Van91I CCANNNNNTGG 2 cut(s) 660, 1953
VpaK11BI GGWCC 4 cut(s) 105, 354, 1864, 1985
VspI ATTAAT 1 cut(s) 1086
XapI RAATTY 1 cut(s) 1663
XceI RCATGY 2 cut(s) 1456, 1802
XmiI GTMKAC 1 cut(s) 1893
XspI CTAG 3 cut(s) 236, 1196, 1310
ZrmI AGTACT 1 cut(s) 812
Zsp2I ATGCAT 1 cut(s) 1749
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.