Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0011819

Physical Location & Seq

Genomic Coordinates Reverse (-)

28144010 .. 28148638

Gene Structure 4629 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1203800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2034 bp

ATGACGATTTTGGGCAATGGCGATTGGCTGCGAATCCCTTTGATCCTGAAAGTTTGGGGGTTTGTGATGATAGCAGTCACCTGCTTCTACCTCGGGAAGCACTGGTCCGATGGGTCCCAGCAGCTCCTCTTCTTCACCACCACCACCACCACCACGCGTCGAGCACCCTCCGCCGTCTCCGTCTCGCCCAATCTAGACAAGCCTTTCAATCTCTCCGCCCTCATAAACGAAACCCTAGCTCCACCTCCACTGCTTCCGCCTCCGCCTCCTCCGTCTCCGCCTCCTCCTTCTTTGCCGCCTCCGCCACCGCCCGATGTGGTCCGGAGGTTCGGGATCGTCGACGAGAACGGCACCATGTCCAACGAGTTCGAGGTGGGGGATTTTGATCCCGATGAGGTGGTGGAGAATTGGGGCAGCGAGAACGGGACTGTGCCCGAGAGCGAACCGAGTAGGTCGGGTCGGGTTAGTGTGAAGAGGTTCGAGCTCTGCCCGAAGAGTATGAGCGAGTACATACCGTGTTTGGATAATGTGGAGGCGATTAAGAGGCTGAAATCGACGGTAAAAGGAGAGCGGTTCGAGCGGCATTGCCCGGAGGCTGGTCGGGGTTTGAATTGCTTGGTTCCGCCGCCCAGGAGTTACAAGACCCCAATTCCATGGCCTAAAAGCCGGGACGAGGTTTGGTACTTCAACGTACCTCATACAAAATTAGTGGAAGATAAAGGGGGTCAGAACTGGATTACCAGACAGAAGAACAAGTTTAAGTTTCCGGGAGGTGGCACACAGTTCATACATGGAGCAAATGGTTACTTGACTGATATTTCTAAGGCGGTCCCTGACATCACCTTTGGTCAGCATATACGAGTTGTTTTGGATGTTGGATGCGGTGTTGCGAGTTTTGGTGCGTATCTGCTGTCGCGAGATGTCCTTACCATGTCAGTTGCACCTAAAGACGTCCATGAGAACCAGATTCAATTTGCTCTTGAACGTGGTGTGCCTGCCATGGTAGCAGCATTTGCAACTCACCGTTTGTTGTACCCGAGTCAGGCATTTGACTTGATACATTGCTCACGATGTAGGATCAACTGGACTCGTGACGATGGCATATTGTTGCTTGAGGTTAATAGGATGCTAAGGGCAGGAGGATACTTTGCTTGGGCAGCACAGCCAGTTTATAAGCACAAAGACGTTCTAGAGGAGCAGTGGCAAGAGATGCTTAACCTCACCAAGCGTCTTTGCTGGGAATTGGTGTCGAAGCAAGGCTACGTTGCAATATGGAAAAAGCCGGTTAACAACAGCTGTTATCTGAATCGGGATGAAGGAACCAAACCTCCATTATGTGATCCAAGTGATGACCCAGACAAAGTTTGGTATGTTGATCTGAAGTCATGCATTACAAGACTCCCTGAAAATGGTTATGGAGCAAATGTCACAGCATGGCCTGCGCGTTTGCAAACTCCCCCTGATCGGCTTCAGAGCATACAACTTGATGCCTATACATCCAGAAAGGAGCTCTTCAAAGCAGAATCCAGATATAACGATGAATTAATTGGAGGTTACGTTCGTGGTTTAAAATGGAAGAAGTTTAAATTAAGAAACGTAATGGACATGAGAGCTGGCTTTGGAGGATTTGCAGCGGCACTAATTGAACATGATTTGAATTGCTGGGTTATGAATGTGGTTCCTGTTAGCGGCCCCAACACCTTACCTGTTATCTATGACCGTGGACTCATTGGAGTAATGCATGACTGGTGTGAACCATTTGATACGTACCCACGGACCTATGATTTATTGCATGCAGCCGGTCTTTTCTCTATTGAGAGAAAAAGATGCGATATCTCTACAATCATGCTTGAGATGGACCGGATGCTAAGACCTGGTGGTCGTGTATACATCCGCGACTCTCTTTCTATCATGGATGAACTTCAAGCGATCGGAAACGCCATGGGTTGGCGCGTCAGTATAAGAGACACATCTGAGGGGCCTCATGCAAGTTATAGACTCTTTATTGCCGAAAAGCGCCTCTTGCATGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

678

Amino Acids

76.42

Weight (kDa)

8.24

Isoelectric Point (pI)

42.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	169 - 672	5.6e-229	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25	PF13649	289 - 381	5.6e-06	Methyltransferase domain
Methyltransf_11	PF08241	290 - 384	1.1e-06	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39750 AT5G06050
fragaria_vesca	FvH4_7g23080 FvH4_7g23080
malus_domestica	MD01G1139400.v1.1 MD07G1203800.v1.1
prunus_persica	Prupe.2G239900_v2.0.a1
pyrus_communis	pycom01g16000 pycom07g19040
rosa_chinensis	RchiOBHm_Chr1g0368331
rosa_laevigata	RLG00000027219
rosa_roxburghii	Rroxscaffold_4G00288640
rosa_rugosa	Rorug01G0341200 Rorug01G0341200
rosa_samantha	Rh1AG347800 Rh1BG310800 Rh1CG326200 Rh1DG342600
rosa_wichuraiana	Rw1G030960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1173
AarI	CACCTGC	1 cut(s)	89
AasI	GACNNNNNNGTC	1 cut(s)	1878
AatII	GACGTC	1 cut(s)	954
Acc36I	ACCTGC	1 cut(s)	89
AccB1I	GGYRCC	1 cut(s)	350
AccB7I	CCANNNNNTGG	1 cut(s)	1947
AccBSI	CCGCTC	2 cut(s)	571, 580
AccI	GTMKAC	2 cut(s)	339, 1887
AccII	CGCG	5 cut(s)	157, 916, 1444, 1896, 1953
AccIII	TCCGGA	1 cut(s)	321
AclWI	GGATC	5 cut(s)	37, 341, 380, 1085, 1334
AcuI	CTGAAG	2 cut(s)	1400, 1454
AcyI	GRCGYC	1 cut(s)	951
AfaI	GTAC	5 cut(s)	509, 683, 693, 1034, 1769
AfiI	CCNNNNNNNGG	8 cut(s)	596, 673, 773, 1042, 1409, 1464, 1688, 1947
AflIII	ACRYGT	1 cut(s)	155
AgsI	TTSAA	9 cut(s)	208, 610, 688, 971, 983, 1516, 1646, 1657, 1925
AjnI	CCWGG	2 cut(s)	629, 1873
AloI	GAACNNNNNNTCC	4 cut(s)	1312, 1344, 1542, 1574
AluBI	AGCT	6 cut(s)	124, 239, 484, 1296, 1510, 1613
AluI	AGCT	6 cut(s)	124, 239, 484, 1296, 1510, 1613
Alw21I	GWGCWC	3 cut(s)	166, 486, 1512
Alw26I	GTCTC	4 cut(s)	181, 187, 279, 1959
AlwI	GGATC	5 cut(s)	37, 341, 380, 1085, 1334
Ama87I	CYCGRG	3 cut(s)	92, 434, 1036
Aor13HI	TCCGGA	1 cut(s)	321
AoxI	GGCC	4 cut(s)	656, 1436, 1690, 1979
ApeKI	GCWGC	7 cut(s)	28, 121, 414, 1007, 1157, 1631, 1796
ArsI	GACNNNNNNTTYG	2 cut(s)	827, 859
AseI	ATTAAT	1 cut(s)	1544
Asp700I	GAANNNNTTC	1 cut(s)	476
AspLEI	GCGC	3 cut(s)	1444, 1953, 2019
AspS9I	GGNCC	8 cut(s)	105, 114, 319, 829, 1691, 1776, 1858, 1979
AsuC2I	CCSGG	3 cut(s)	590, 668, 768
AsuHPI	GGTGA	5 cut(s)	70, 127, 832, 1013, 1213
AvaI	CYCGRG	3 cut(s)	92, 434, 1036
AvaII	GGWCC	6 cut(s)	105, 114, 319, 829, 1776, 1858
BaeGI	GKGCMC	1 cut(s)	435
BanI	GGYRCC	1 cut(s)	350
BanII	GRGCYC	2 cut(s)	486, 1512
BarI	GAAGNNNNNNTAC	2 cut(s)	1244, 1276
BauI	CACGAG	1 cut(s)	1089
Bbv12I	GWGCWC	3 cut(s)	166, 486, 1512
BbvI	GCAGC	7 cut(s)	15, 133, 426, 1019, 1169, 1643, 1808
BccI	CCATC	3 cut(s)	104, 1091, 1849
BceAI	ACGGC	2 cut(s)	158, 364
BciT130I	CCWGG	2 cut(s)	631, 1875
BciVI	GTATCC	1 cut(s)	1136
BcnI	CCSGG	3 cut(s)	590, 668, 768
BcoDI	GTCTC	4 cut(s)	181, 187, 279, 1959
BfaI	CTAG	3 cut(s)	194, 236, 1190
BfoI	RGCGCY	1 cut(s)	2020
BfuAI	ACCTGC	1 cut(s)	89
BfuI	GTATCC	1 cut(s)	1136
Bme1390I	CCNGG	5 cut(s)	590, 631, 668, 768, 1875
Bme18I	GGWCC	6 cut(s)	105, 114, 319, 829, 1776, 1858
BmeT110I	CYCGRG	3 cut(s)	92, 434, 1036
BmgT120I	GGNCC	8 cut(s)	105, 114, 319, 829, 1691, 1776, 1858, 1979
BmiI	GGNNCC	9 cut(s)	115, 116, 352, 621, 831, 1321, 1680, 1693, 1980
BmrFI	CCNGG	5 cut(s)	590, 631, 668, 768, 1875
BmsI	GCATC	6 cut(s)	869, 1116, 1200, 1477, 1817, 1854
Bpu10I	CCTNAGC	1 cut(s)	1130
BpuEI	CTTGAG	2 cut(s)	1133, 1871
BpuMI	CCSGG	3 cut(s)	590, 668, 768
BsaAI	YACGTR	1 cut(s)	1767
BsaBI	GATNNNNATC	1 cut(s)	384
BsaHI	GRCGYC	1 cut(s)	951
BsaJI	CCNNGG	7 cut(s)	91, 629, 653, 999, 1720, 1772, 1941
BsaWI	WCCGGW	2 cut(s)	321, 1860
BsaXI	ACNNNNNCTCC	8 cut(s)	223, 253, 1312, 1342, 1410, 1440, 1542, 1572
Bsc4I	CCNNNNNNNGG	8 cut(s)	596, 673, 773, 1042, 1409, 1464, 1688, 1947
Bse118I	RCCGGY	2 cut(s)	1282, 1799
Bse1I	ACTGG	5 cut(s)	107, 737, 1088, 1166, 1751
Bse3DI	GCAATG	3 cut(s)	22, 583, 1060
Bse8I	GATNNNNATC	1 cut(s)	384
BseAI	TCCGGA	1 cut(s)	321
BseBI	CCWGG	2 cut(s)	631, 1875
BseDI	CCNNGG	7 cut(s)	91, 629, 653, 999, 1720, 1772, 1941
BseGI	GGATG	8 cut(s)	877, 884, 1131, 1318, 1496, 1869, 1890, 1921
BseJI	GATNNNNATC	1 cut(s)	384
BseLI	CCNNNNNNNGG	8 cut(s)	596, 673, 773, 1042, 1409, 1464, 1688, 1947
BseMI	GCAATG	3 cut(s)	22, 583, 1060
BseMII	CTCAG	1 cut(s)	1965
BseNI	ACTGG	5 cut(s)	107, 737, 1088, 1166, 1751
BseRI	GAGGAG	4 cut(s)	116, 258, 273, 1208
BseSI	GKGCMC	1 cut(s)	435
BseXI	GCAGC	7 cut(s)	15, 133, 426, 1019, 1169, 1643, 1808
BseYI	CCCAGC	3 cut(s)	117, 1236, 1662
Bsh1236I	CGCG	5 cut(s)	157, 916, 1444, 1896, 1953
Bsh1285I	CGRYCG	1 cut(s)	1932
BshFI	GGCC	4 cut(s)	658, 1438, 1692, 1981
BshNI	GGYRCC	1 cut(s)	350
BsiEI	CGRYCG	1 cut(s)	1932
BsiHKAI	GWGCWC	3 cut(s)	166, 486, 1512
BsiHKCI	CYCGRG	3 cut(s)	92, 434, 1036
BsiSI	CCGG	7 cut(s)	322, 590, 667, 767, 1283, 1800, 1861
BslFI	GGGAC	4 cut(s)	100, 439, 683, 815
BslI	CCNNNNNNNGG	8 cut(s)	596, 673, 773, 1042, 1409, 1464, 1688, 1947
BsmAI	GTCTC	4 cut(s)	181, 187, 279, 1959
BsmBI	CGTCTC	3 cut(s)	181, 187, 279
BsmFI	GGGAC	4 cut(s)	100, 439, 683, 815
BsnI	GGCC	4 cut(s)	658, 1438, 1692, 1981
BsoBI	CYCGRG	3 cut(s)	92, 434, 1036
Bsp1286I	GDGCHC	4 cut(s)	166, 435, 486, 1512
Bsp13I	TCCGGA	1 cut(s)	321
Bsp143I	GATC	8 cut(s)	42, 333, 385, 1077, 1339, 1375, 1462, 1929
Bsp19I	CCATGG	3 cut(s)	653, 999, 1941
Bsp68I	TCGCGA	1 cut(s)	916
BspANI	GGCC	4 cut(s)	658, 1438, 1692, 1981
BspCNI	CTCAG	1 cut(s)	1966
BspEI	TCCGGA	1 cut(s)	321
BspFNI	CGCG	5 cut(s)	157, 916, 1444, 1896, 1953
BspLI	GGNNCC	9 cut(s)	115, 116, 352, 621, 831, 1321, 1680, 1693, 1980
BspMI	ACCTGC	1 cut(s)	89
BspPI	GGATC	5 cut(s)	37, 341, 380, 1085, 1334
BspQI	GCTCTTC	1 cut(s)	1517
BspT107I	GGYRCC	1 cut(s)	350
BsrBI	CCGCTC	2 cut(s)	571, 580
BsrDI	GCAATG	3 cut(s)	22, 583, 1060
BsrFI	RCCGGY	2 cut(s)	1282, 1799
BsrI	ACTGG	5 cut(s)	107, 737, 1088, 1166, 1751
BssAI	RCCGGY	2 cut(s)	1282, 1799
BssECI	CCNNGG	7 cut(s)	91, 629, 653, 999, 1720, 1772, 1941
BssMI	GATC	8 cut(s)	42, 333, 385, 1077, 1339, 1375, 1462, 1929
BssNAI	GTATAC	1 cut(s)	1888
BssNI	GRCGYC	1 cut(s)	951
BssSI	CACGAG	1 cut(s)	1089
BssT1I	CCWWGG	3 cut(s)	653, 999, 1941
Bst1107I	GTATAC	1 cut(s)	1888
Bst2BI	CACGAG	1 cut(s)	1089
Bst2UI	CCWGG	2 cut(s)	631, 1875
Bst4CI	ACNGT	6 cut(s)	430, 516, 559, 783, 1025, 1721
Bst6I	CTCTTC	4 cut(s)	134, 467, 488, 1517
BstACI	GRCGYC	1 cut(s)	951
BstAPI	GCANNNNNTGC	3 cut(s)	1013, 1210, 1439
BstBAI	YACGTR	1 cut(s)	1767
BstC8I	GCNNGC	4 cut(s)	996, 1440, 1615, 1794
BstDEI	CTNAG	4 cut(s)	822, 1130, 1868, 1974
BstDSI	CCRYGG	5 cut(s)	653, 999, 1720, 1772, 1941
BstF5I	GGATG	8 cut(s)	877, 884, 1131, 1318, 1496, 1869, 1890, 1921
BstFNI	CGCG	5 cut(s)	157, 916, 1444, 1896, 1953
BstH2I	RGCGCY	1 cut(s)	2020
BstHHI	GCGC	3 cut(s)	1444, 1953, 2019
BstKTI	GATC	8 cut(s)	45, 336, 388, 1080, 1342, 1378, 1465, 1932
BstMAI	GTCTC	4 cut(s)	181, 187, 279, 1959
BstMBI	GATC	8 cut(s)	42, 333, 385, 1077, 1339, 1375, 1462, 1929
BstMCI	CGRYCG	1 cut(s)	1932
BstMWI	GCNNNNNNNGC	9 cut(s)	170, 301, 577, 1004, 1013, 1157, 1210, 1439, 2023
BstNI	CCWGG	2 cut(s)	631, 1875
BstNSI	RCATGY	1 cut(s)	1796
BstSCI	CCNGG	5 cut(s)	588, 629, 666, 766, 1873
BstSLI	GKGCMC	1 cut(s)	435
BstSNI	TACGTA	1 cut(s)	1767
BstUI	CGCG	5 cut(s)	157, 916, 1444, 1896, 1953
BstV1I	GCAGC	7 cut(s)	15, 133, 426, 1019, 1169, 1643, 1808
BstXI	CCANNNNNNTGG	1 cut(s)	654
BstZ17I	GTATAC	1 cut(s)	1888
BsuI	GTATCC	1 cut(s)	1136
BsuRI	GGCC	4 cut(s)	658, 1438, 1692, 1981
BtgI	CCRYGG	5 cut(s)	653, 999, 1720, 1772, 1941
BtsCI	GGATG	8 cut(s)	877, 884, 1131, 1318, 1496, 1869, 1890, 1921
BtsI	GCAGTG	2 cut(s)	248, 1205
BtsIMutI	CAGTG	3 cut(s)	100, 248, 1205
BtuMI	TCGCGA	1 cut(s)	916
BveI	ACCTGC	1 cut(s)	89
Cac8I	GCNNGC	4 cut(s)	996, 1440, 1615, 1794
CfoI	GCGC	3 cut(s)	1444, 1953, 2019
Cfr10I	RCCGGY	2 cut(s)	1282, 1799
Cfr13I	GGNCC	8 cut(s)	105, 114, 319, 829, 1691, 1776, 1858, 1979
CseI	GACGC	3 cut(s)	146, 1217, 1942
CsiI	ACCWGGT	1 cut(s)	1873
Csp6I	GTAC	5 cut(s)	508, 682, 692, 1033, 1768
CspCI	CAANNNNNGTGG	2 cut(s)	690, 725
CviQI	GTAC	5 cut(s)	508, 682, 692, 1033, 1768
DdeI	CTNAG	4 cut(s)	822, 1130, 1868, 1974
DpnI	GATC	8 cut(s)	44, 335, 387, 1079, 1341, 1377, 1464, 1931
DpnII	GATC	8 cut(s)	42, 333, 385, 1077, 1339, 1375, 1462, 1929
DraI	TTTAAA	2 cut(s)	1569, 1585
DrdI	GACNNNNNNGTC	1 cut(s)	1878
DseDI	GACNNNNNNGTC	1 cut(s)	1878
Eam1104I	CTCTTC	4 cut(s)	134, 467, 488, 1517
EarI	CTCTTC	4 cut(s)	134, 467, 488, 1517
EciI	GGCGGA	7 cut(s)	160, 205, 246, 252, 267, 291, 612
Ecl136II	GAGCTC	2 cut(s)	484, 1510
Eco105I	TACGTA	1 cut(s)	1767
Eco130I	CCWWGG	3 cut(s)	653, 999, 1941
Eco24I	GRGCYC	2 cut(s)	486, 1512
Eco32I	GATATC	1 cut(s)	1834
Eco47I	GGWCC	6 cut(s)	105, 114, 319, 829, 1776, 1858
Eco53kI	GAGCTC	2 cut(s)	484, 1510
Eco57I	CTGAAG	2 cut(s)	1400, 1454
Eco88I	CYCGRG	3 cut(s)	92, 434, 1036
EcoICRI	GAGCTC	2 cut(s)	484, 1510
EcoO109I	RGGNCCY	2 cut(s)	114, 1979
EcoRII	CCWGG	2 cut(s)	629, 1873
EcoRV	GATATC	1 cut(s)	1834
EcoT14I	CCWWGG	3 cut(s)	653, 999, 1941
EcoT22I	ATGCAT	2 cut(s)	1391, 1743
EcoT38I	GRGCYC	2 cut(s)	486, 1512
ErhI	CCWWGG	3 cut(s)	653, 999, 1941
Esp3I	CGTCTC	3 cut(s)	181, 187, 279
FalI	AAGNNNNNCTT	5 cut(s)	1197, 1229, 1496, 1528, 2006
FaqI	GGGAC	4 cut(s)	100, 439, 683, 815
FblI	GTMKAC	2 cut(s)	339, 1887
FokI	GGATG	8 cut(s)	884, 891, 1138, 1325, 1483, 1876, 1877, 1928
FriOI	GRGCYC	2 cut(s)	486, 1512
FspBI	CTAG	3 cut(s)	194, 236, 1190
GlaI	GCGC	3 cut(s)	1443, 1952, 2018
GsaI	CCCAGC	3 cut(s)	121, 1240, 1666
HaeII	RGCGCY	1 cut(s)	2020
HaeIII	GGCC	4 cut(s)	658, 1438, 1692, 1981
HapII	CCGG	7 cut(s)	322, 590, 667, 767, 1283, 1800, 1861
HgaI	GACGC	3 cut(s)	146, 1217, 1942
HhaI	GCGC	3 cut(s)	1444, 1953, 2019
Hin1I	GRCGYC	1 cut(s)	951
Hin6I	GCGC	3 cut(s)	1442, 1951, 2017
HinP1I	GCGC	3 cut(s)	1442, 1951, 2017
HincII	GTYRAC	2 cut(s)	340, 1288
HindII	GTYRAC	2 cut(s)	340, 1288
HpaI	GTTAAC	1 cut(s)	1288
HpaII	CCGG	7 cut(s)	322, 590, 667, 767, 1283, 1800, 1861
HphI	GGTGA	5 cut(s)	70, 127, 832, 1013, 1213
Hpy166II	GTNNAC	5 cut(s)	340, 1288, 1724, 1754, 1888
Hpy188I	TCNGA	7 cut(s)	109, 729, 1305, 1380, 1473, 1934, 1975
Hpy8I	GTNNAC	5 cut(s)	340, 1288, 1724, 1754, 1888
Hpy99I	CGWCG	4 cut(s)	162, 341, 344, 559
HpyAV	CCTTC	2 cut(s)	297, 1310
HpyCH4III	ACNGT	6 cut(s)	430, 516, 559, 783, 1025, 1721
HpyCH4IV	ACGT	8 cut(s)	690, 951, 985, 1185, 1263, 1557, 1596, 1766
HpyF10VI	GCNNNNNNNGC	9 cut(s)	170, 301, 577, 1004, 1013, 1157, 1210, 1439, 2023
HpyF3I	CTNAG	4 cut(s)	822, 1130, 1868, 1974
HpySE526I	ACGT	8 cut(s)	690, 951, 985, 1185, 1263, 1557, 1596, 1766
Hsp92I	GRCGYC	1 cut(s)	951
HspAI	GCGC	3 cut(s)	1442, 1951, 2017
KflI	GGGWCCC	1 cut(s)	114
Kpn2I	TCCGGA	1 cut(s)	321
KspAI	GTTAAC	1 cut(s)	1288
Kzo9I	GATC	8 cut(s)	42, 333, 385, 1077, 1339, 1375, 1462, 1929
LguI	GCTCTTC	1 cut(s)	1517
LmnI	GCTCC	6 cut(s)	129, 244, 794, 1195, 1418, 1507
Lsp1109I	GCAGC	7 cut(s)	15, 133, 426, 1019, 1169, 1643, 1808
LweI	GCATC	6 cut(s)	869, 1116, 1200, 1477, 1817, 1854
MabI	ACCWGGT	1 cut(s)	1873
MaeI	CTAG	3 cut(s)	194, 236, 1190
MaeII	ACGT	8 cut(s)	690, 951, 985, 1185, 1263, 1557, 1596, 1766
MaeIII	GTNAC	6 cut(s)	76, 635, 803, 1091, 1426, 1553
MalI	GATC	8 cut(s)	44, 335, 387, 1079, 1341, 1377, 1464, 1931
MbiI	CCGCTC	2 cut(s)	571, 580
MboI	GATC	8 cut(s)	42, 333, 385, 1077, 1339, 1375, 1462, 1929
MboII	GAAGA	8 cut(s)	121, 124, 484, 505, 725, 760, 1504, 1588
MhlI	GDGCHC	4 cut(s)	166, 435, 486, 1512
MluI	ACGCGT	1 cut(s)	155
MlyI	GAGTC	6 cut(s)	1048, 1081, 1392, 1719, 1892, 1992
MmeI	TCCRAC	2 cut(s)	384, 856
Mph1103I	ATGCAT	2 cut(s)	1391, 1743
MroI	TCCGGA	1 cut(s)	321
MroXI	GAANNNNTTC	1 cut(s)	476
MseI	TTAA	9 cut(s)	540, 759, 1119, 1215, 1287, 1544, 1568, 1584, 1589
MspA1I	CMGCKG	2 cut(s)	1296, 1634
MspI	CCGG	7 cut(s)	322, 590, 667, 767, 1283, 1800, 1861
MspR9I	CCNGG	5 cut(s)	590, 631, 668, 768, 1875
MvaI	CCWGG	2 cut(s)	631, 1875
MvnI	CGCG	5 cut(s)	157, 916, 1444, 1896, 1953
MwoI	GCNNNNNNNGC	9 cut(s)	170, 301, 577, 1004, 1013, 1157, 1210, 1439, 2023
NciI	CCSGG	3 cut(s)	590, 668, 768
NcoI	CCATGG	3 cut(s)	653, 999, 1941
NdeII	GATC	8 cut(s)	42, 333, 385, 1077, 1339, 1375, 1462, 1929
NlaIV	GGNNCC	9 cut(s)	115, 116, 352, 621, 831, 1321, 1680, 1693, 1980
NmuCI	GTSAC	3 cut(s)	76, 1091, 1426
NruI	TCGCGA	1 cut(s)	916
NsiI	ATGCAT	2 cut(s)	1391, 1743
NspI	RCATGY	1 cut(s)	1796
PaeI	GCATGC	1 cut(s)	1796
PaqCI	CACCTGC	1 cut(s)	89
PciSI	GCTCTTC	1 cut(s)	1517
PcsI	WCGNNNNNNNCGW	1 cut(s)	336
PdmI	GAANNNNTTC	1 cut(s)	476
PfeI	GAWTC	4 cut(s)	33, 967, 1306, 1523
PflMI	CCANNNNNTGG	1 cut(s)	1947
PfoI	TCCNGGA	1 cut(s)	766
Ple19I	CGATCG	1 cut(s)	1932
PleI	GAGTC	6 cut(s)	1047, 1081, 1392, 1719, 1892, 1992
PpsI	GAGTC	6 cut(s)	1047, 1081, 1392, 1719, 1892, 1992
Ppu21I	YACGTR	1 cut(s)	1767
PpuMI	RGGWCCY	1 cut(s)	114
PshBI	ATTAAT	1 cut(s)	1544
PsiI	TTATAA	1 cut(s)	1173
Psp124BI	GAGCTC	2 cut(s)	486, 1512
Psp5II	RGGWCCY	1 cut(s)	114
Psp6I	CCWGG	2 cut(s)	629, 1873
PspFI	CCCAGC	3 cut(s)	117, 1236, 1662
PspGI	CCWGG	2 cut(s)	629, 1873
PspN4I	GGNNCC	9 cut(s)	115, 116, 352, 621, 831, 1321, 1680, 1693, 1980
PspPI	GGNCC	8 cut(s)	105, 114, 319, 829, 1691, 1776, 1858, 1979
PspPPI	RGGWCCY	1 cut(s)	114
PvuI	CGATCG	1 cut(s)	1932
PvuII	CAGCTG	1 cut(s)	1296
RruI	TCGCGA	1 cut(s)	916
RsaI	GTAC	5 cut(s)	509, 683, 693, 1034, 1769
RsaNI	GTAC	5 cut(s)	508, 682, 692, 1033, 1768
SacI	GAGCTC	2 cut(s)	486, 1512
SalI	GTCGAC	1 cut(s)	338
SapI	GCTCTTC	1 cut(s)	1517
SaqAI	TTAA	9 cut(s)	540, 759, 1119, 1215, 1287, 1544, 1568, 1584, 1589
Sau3AI	GATC	8 cut(s)	42, 333, 385, 1077, 1339, 1375, 1462, 1929
Sau96I	GGNCC	8 cut(s)	105, 114, 319, 829, 1691, 1776, 1858, 1979
SchI	GAGTC	6 cut(s)	1048, 1081, 1392, 1719, 1892, 1992
ScrFI	CCNGG	5 cut(s)	590, 631, 668, 768, 1875
SduI	GDGCHC	4 cut(s)	166, 435, 486, 1512
SexAI	ACCWGGT	1 cut(s)	1873
SfaNI	GCATC	6 cut(s)	869, 1116, 1200, 1477, 1817, 1854
SgrDI	CGTCGACG	1 cut(s)	338
SinI	GGWCC	6 cut(s)	105, 114, 319, 829, 1776, 1858
SmlI	CTYRAG	2 cut(s)	1112, 1850
SmoI	CTYRAG	2 cut(s)	1112, 1850
SnaBI	TACGTA	1 cut(s)	1767
SphI	GCATGC	1 cut(s)	1796
SspMI	CTAG	3 cut(s)	194, 236, 1190
SstI	GAGCTC	2 cut(s)	486, 1512
StyD4I	CCNGG	5 cut(s)	588, 629, 666, 766, 1873
StyI	CCWWGG	3 cut(s)	653, 999, 1941
TaaI	ACNGT	6 cut(s)	430, 516, 559, 783, 1025, 1721
TaiI	ACGT	8 cut(s)	693, 954, 988, 1188, 1266, 1560, 1599, 1769
TaqI	TCGA	7 cut(s)	160, 339, 369, 480, 554, 576, 1250
TatI	WGTACW	1 cut(s)	507
TauI	GCSGC	5 cut(s)	298, 583, 628, 1637, 1692
TfiI	GAWTC	4 cut(s)	33, 967, 1306, 1523
Tru1I	TTAA	9 cut(s)	540, 759, 1119, 1215, 1287, 1544, 1568, 1584, 1589
Tru9I	TTAA	9 cut(s)	540, 759, 1119, 1215, 1287, 1544, 1568, 1584, 1589
TscAI	CASTG	3 cut(s)	107, 255, 1205
TseFI	GTSAC	3 cut(s)	76, 1091, 1426
TseI	GCWGC	7 cut(s)	28, 121, 414, 1007, 1157, 1631, 1796
Tsp45I	GTSAC	3 cut(s)	76, 1091, 1426
TspDTI	ATGAA	5 cut(s)	775, 1329, 1554, 1685, 1932
TspGWI	ACGGA	3 cut(s)	169, 261, 1789
TspRI	CASTG	3 cut(s)	107, 255, 1205
Van91I	CCANNNNNTGG	1 cut(s)	1947
VpaK11BI	GGWCC	6 cut(s)	105, 114, 319, 829, 1776, 1858
VspI	ATTAAT	1 cut(s)	1544
XbaI	TCTAGA	2 cut(s)	193, 1189
XceI	RCATGY	1 cut(s)	1796
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1362
XmiI	GTMKAC	2 cut(s)	339, 1887
XmnI	GAANNNNTTC	1 cut(s)	476
XspI	CTAG	3 cut(s)	194, 236, 1190
ZraI	GACGTC	1 cut(s)	952
Zsp2I	ATGCAT	2 cut(s)	1391, 1743

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.