Rw1G030960

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0011819
Physical Location & Seq
Reverse (-)
58958800 .. 58963020
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G030960.1

Sequence Viewer

Length: 2046 bp
ATGGGATTTTTCGGGAATGGCGACTGGTTGAGGATCCCATTGATCATCAAATTATGGGGGTTTATCATGATCTCCGTCACCTTCTTCTACTTGGGCAAGAACTGGACCGGTGGGGCCCAGCAGCTCCTCTCCTTCACCACCGCCGCCACCACGCGCCGATCTCCTCCTTCCGCCACCGTCTCCATTTCCCCCAATCTCAACAAGCCCTTCATCCTCTCCTCCCTCCTCAACGAAACCCTGGCCCCGCCGCCCTTGCCTTCTCCTCCTCCGCCTCTGCCGCCGCTTCCTTCTTCTCCGCCGCCCCCCGCGCCGCCGCCGTCGGACGTGATCCAGCGGTTCGGGATCGTGGACGAGAACGGGACCATGGCCGACGATTTCGAGGTCGGCGAGTTCGATCCCGATGAGGTGGTGGAGAATTGGAGCGGCCAGAATGGGACGGCGGCGGCGGAGAGTGACGGGAGCGGGTCGGGTAGGTTCAATGGGAAAAGGTTTGAGCTTTGTCGTAACGGTATGAGCGAGTACATACCGTGTTTGGATAATGTGGTGGCCATTAAGAGGCTCAAATCCACCGATAAAGGCGAGCGCTTTGAGCGGCATTGTCCGGATTCCGGCAAGGGTTTGAATTGCTTGGTTCCTCCGCCCAAGAACTACAAGAGCCCCATTCCATGGCCGAAGAGCCGCGATGAGGTTTGGTTCAATAATGTACCTCATACTCGTCTGGTTGAGGATAAAGGGGGTCAGAACTGGATTACACGAGAAAAGAAGAAGTTTAAGTTTCCGGGAGGTGGGACACAGTTTATACATGGGGCAAATGAGTACTTGGATCATATTTCTAAGATGATCCCTGACATCACATTTGGTCACCATATTCGAGTTGCATTGGATGTTGGATGTGGTGTTGCAAGTTTTGGTGCATACCTGCTGCAACGGAATGTCATCACTATGTCAATTGCTCCAAAAGATGTCCATGAGAATCAGATACAATTTGCTCTTGAGCGTGGAGTGCCTGCAATGGTAGCAGCATTTGCTACTCGCCGTTTATTGTACCCAAGTCAGGCATTTGACTTGATCCATTGCTCACGATGCAGGATTAATTGGACTCGTGACGATGGGATATTGCTACTTGAGGTTAATAGGATGCTCCGGGCAGGAGGATACTTTGTTTGGGCAGCACAGCCAGTTTATAAGCATGAAGAAGTCCTAGAGGAGCAGTGGCAAGAGATGCTTAACCTCACGACCCGTCTTTGCTGGGAGTTTGTGAAAAAGGATGGCTATGTTGCCGTATGGCAGAAGCCAGTTAACAACAGCTGCTATCTGGGTCGGGATGCTGAAACCAAACCTCCATTATGCGACTCAAGTGATGACCCAGACAAAGTTTGGTATGTTGATCTGAAGGCATGCATAACAAGACTCCCTGAAAATGGTTATGGAGCAAATGTCACAACATGGCCAGACCGCATGCAAACTCCCCCTGATAGGCTTCAGAGCATACAGTTTGATGCCTATATATCCAGAAAGGAGCTCTTCAAGGCAGAATCTAAATATTGGCATGAAATAATTGAAGGCTATGTCCGTGCTTTTAAATGGAAGAAGTTAAGATTAAGAAATGTAATGGATATGAGAGCTGGCTTTGGAGGATTTGCAGTGGCACTAATGGAACATAATTTAAATTCCTGGGTTATGAATGTGGTTCCTGTTAGCGGCCCCAACACCTTGCCTGTTATATATGACCGTGGATTTATTGGAGTTATGCATGACTGGTGTGAGCCATTTGATACATACCCAAGAACCTATGATTTATTACATGCAGCTGGCCTCTTCTCTATTGAGAAGAAAAGGTGCAATATGTCAACAATCATGCTTGAGATGGACCGTATGCTAAGACCAGGTGGACGTGTATACATCCGTGATGCTCTTGCGGTCATGGATGAGCTTCAAGCGATTGGAAATGCCATGGGCTGGAAAGTTAATTTGCGAGACACATCTGAGGGTCCTCATGCAAGCTATAGGCTCTTCATGGCTGAAAAACGCCTCTTGCATAGTTGA

Protein Analysis

681

Amino Acids

77.04

Weight (kDa)

7.88

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 173 - 676 5.7e-232 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 294 - 388 7.6e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39750 AT5G06050
fragaria_vesca FvH4_7g23080 FvH4_7g23080
malus_domestica MD01G1139400.v1.1 MD07G1203800.v1.1
prunus_persica Prupe.2G239900_v2.0.a1
pyrus_communis pycom01g16000 pycom07g19040
rosa_chinensis RchiOBHm_Chr1g0368331
rosa_laevigata RLG00000027219
rosa_roxburghii Rroxscaffold_4G00288640
rosa_rugosa Rorug01G0341200 Rorug01G0341200
rosa_samantha Rh1AG347800 Rh1BG310800 Rh1CG326200 Rh1DG342600
rosa_wichuraiana Rw1G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1185
Acc36I ACCTGC 1 cut(s) 927
AccB7I CCANNNNNTGG 2 cut(s) 666, 1959
AccBSI CCGCTC 3 cut(s) 423, 462, 592
AccI GTMKAC 1 cut(s) 1899
AccII CGCG 3 cut(s) 154, 308, 681
AccIII TCCGGA 1 cut(s) 601
AclWI GGATC 8 cut(s) 28, 41, 322, 350, 389, 831, 835, 1063
AcoI YGGCCR 5 cut(s) 366, 424, 546, 668, 1448
AcsI RAATTY 1 cut(s) 1669
AcuI CTGAAG 2 cut(s) 1412, 1466
AfaI GTAC 4 cut(s) 521, 705, 818, 1046
AfeI AGCGCT 1 cut(s) 584
AflIII ACRYGT 1 cut(s) 1894
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 6 cut(s) 478, 622, 697, 1528, 1562, 1937
AjiI CACGTC 2 cut(s) 325, 1895
AjnI CCWGG 3 cut(s) 237, 1673, 1885
AluBI AGCT 8 cut(s) 124, 496, 1308, 1522, 1625, 1811, 1933, 2004
AluI AGCT 8 cut(s) 124, 496, 1308, 1522, 1625, 1811, 1933, 2004
Alw21I GWGCWC 1 cut(s) 1524
Alw26I GTCTC 2 cut(s) 184, 1971
AlwI GGATC 8 cut(s) 28, 41, 322, 350, 389, 831, 835, 1063
Aor13HI TCCGGA 1 cut(s) 601
Aor51HI AGCGCT 1 cut(s) 584
AoxI GGCC 9 cut(s) 114, 240, 366, 424, 546, 668, 1448, 1702, 1813
ApaI GGGCCC 1 cut(s) 118
ApeKI GCWGC 6 cut(s) 121, 922, 1019, 1169, 1308, 1808
ApoI RAATTY 1 cut(s) 1669
ArsI GACNNNNNNTTYG 2 cut(s) 839, 871
AseI ATTAAT 1 cut(s) 1092
AsiGI ACCGGT 1 cut(s) 107
AspLEI GCGC 3 cut(s) 156, 310, 585
AspS9I GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1703, 1870, 1991
AsuC2I CCSGG 2 cut(s) 780, 1145
AsuHPI GGTGA 3 cut(s) 70, 127, 854
AvaII GGWCC 4 cut(s) 105, 360, 1870, 1991
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 2 cut(s) 548, 1450
BamHI GGATCC 1 cut(s) 33
BanII GRGCYC 3 cut(s) 118, 659, 1524
BauI CACGAG 2 cut(s) 753, 1101
Bbv12I GWGCWC 1 cut(s) 1524
BbvI GCAGC 6 cut(s) 133, 909, 1031, 1181, 1295, 1820
BccI CCATC 3 cut(s) 1103, 1262, 1861
BceAI ACGGC 4 cut(s) 301, 453, 1020, 1265
BciT130I CCWGG 3 cut(s) 239, 1675, 1887
BciVI GTATCC 1 cut(s) 1148
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 2 cut(s) 780, 1145
BcoDI GTCTC 2 cut(s) 184, 1971
BfaI CTAG 1 cut(s) 1202
BfmI CTRYAG 1 cut(s) 2005
BfoI RGCGCY 1 cut(s) 586
BfuAI ACCTGC 1 cut(s) 927
BfuI GTATCC 1 cut(s) 1148
BmcAI AGTACT 1 cut(s) 818
Bme1390I CCNGG 5 cut(s) 239, 780, 1145, 1675, 1887
Bme18I GGWCC 4 cut(s) 105, 360, 1870, 1991
BmgBI CACGTC 2 cut(s) 325, 1895
BmgT120I GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1703, 1870, 1991
BmiI GGNNCC 9 cut(s) 35, 115, 116, 243, 361, 633, 1692, 1705, 1992
BmrFI CCNGG 5 cut(s) 239, 780, 1145, 1675, 1887
BmsI GCATC 6 cut(s) 1073, 1128, 1212, 1315, 1489, 1900
BpuEI CTTGAG 4 cut(s) 1013, 1145, 1339, 1883
BpuMI CCSGG 2 cut(s) 780, 1145
BsaJI CCNNGG 6 cut(s) 237, 363, 665, 1674, 1732, 1953
BsaWI WCCGGW 2 cut(s) 107, 601
BsaXI ACNNNNNCTCC 4 cut(s) 1324, 1354, 1422, 1452
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 6 cut(s) 29, 107, 749, 1178, 1295, 1763
Bse3DI GCAATG 2 cut(s) 1017, 1072
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 3 cut(s) 239, 1675, 1887
BseDI CCNNGG 6 cut(s) 237, 363, 665, 1674, 1732, 1953
BseGI GGATG 8 cut(s) 210, 889, 896, 1143, 1273, 1330, 1902, 1933
BseMI GCAATG 2 cut(s) 1017, 1072
BseMII CTCAG 1 cut(s) 1977
BseNI ACTGG 6 cut(s) 29, 107, 749, 1178, 1295, 1763
BseRI GAGGAG 7 cut(s) 116, 153, 208, 215, 252, 255, 1220
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 6 cut(s) 133, 909, 1031, 1181, 1295, 1820
BseYI CCCAGC 2 cut(s) 117, 1248
Bsh1236I CGCG 3 cut(s) 154, 308, 681
BshFI GGCC 9 cut(s) 116, 242, 368, 426, 548, 670, 1450, 1704, 1815
BshTI ACCGGT 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 1524
BsiSI CCGG 5 cut(s) 108, 602, 609, 779, 1144
BslFI GGGAC 3 cut(s) 373, 448, 802
BsmAI GTCTC 2 cut(s) 184, 1971
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 3 cut(s) 373, 448, 802
BsnI GGCC 9 cut(s) 116, 242, 368, 426, 548, 670, 1450, 1704, 1815
Bsp120I GGGCCC 1 cut(s) 114
Bsp1286I GDGCHC 3 cut(s) 118, 659, 1524
Bsp13I TCCGGA 1 cut(s) 601
Bsp19I CCATGG 3 cut(s) 363, 665, 1953
BspANI GGCC 9 cut(s) 116, 242, 368, 426, 548, 670, 1450, 1704, 1815
BspCNI CTCAG 1 cut(s) 1978
BspEI TCCGGA 1 cut(s) 601
BspFNI CGCG 3 cut(s) 154, 308, 681
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 9 cut(s) 35, 115, 116, 243, 361, 633, 1692, 1705, 1992
BspMI ACCTGC 1 cut(s) 927
BspPI GGATC 8 cut(s) 28, 41, 322, 350, 389, 831, 835, 1063
BspQI GCTCTTC 3 cut(s) 668, 1529, 2018
BsrBI CCGCTC 3 cut(s) 423, 462, 592
BsrDI GCAATG 2 cut(s) 1017, 1072
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 6 cut(s) 29, 107, 749, 1178, 1295, 1763
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 6 cut(s) 237, 363, 665, 1674, 1732, 1953
BssNAI GTATAC 1 cut(s) 1900
BssSI CACGAG 2 cut(s) 753, 1101
BssT1I CCWWGG 3 cut(s) 363, 665, 1953
Bst1107I GTATAC 1 cut(s) 1900
Bst2BI CACGAG 2 cut(s) 753, 1101
Bst2UI CCWGG 3 cut(s) 239, 1675, 1887
Bst4CI ACNGT 7 cut(s) 178, 509, 528, 795, 1494, 1733, 1874
Bst6I CTCTTC 4 cut(s) 668, 1529, 1823, 2018
BstAPI GCANNNNNTGC 2 cut(s) 1025, 1222
BstC8I GCNNGC 7 cut(s) 581, 1008, 1399, 1460, 1627, 1813, 2002
BstDEI CTNAG 3 cut(s) 834, 1880, 1986
BstDSI CCRYGG 4 cut(s) 363, 665, 1732, 1953
BstEII GGTNACC 1 cut(s) 860
BstF5I GGATG 8 cut(s) 210, 889, 896, 1143, 1273, 1330, 1902, 1933
BstFNI CGCG 3 cut(s) 154, 308, 681
BstH2I RGCGCY 1 cut(s) 586
BstHHI GCGC 3 cut(s) 156, 310, 585
BstMAI GTCTC 2 cut(s) 184, 1971
BstMWI GCNNNNNNNGC 9 cut(s) 253, 277, 307, 589, 1003, 1016, 1025, 1083, 1222
BstNI CCWGG 3 cut(s) 239, 1675, 1887
BstNSI RCATGY 3 cut(s) 1401, 1462, 1808
BstPI GGTNACC 1 cut(s) 860
BstSCI CCNGG 5 cut(s) 237, 778, 1143, 1673, 1885
BstSFI CTRYAG 1 cut(s) 2005
BstSLI GKGCMC 1 cut(s) 118
BstUI CGCG 3 cut(s) 154, 308, 681
BstV1I GCAGC 6 cut(s) 133, 909, 1031, 1181, 1295, 1820
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BstZ17I GTATAC 1 cut(s) 1900
BsuI GTATCC 1 cut(s) 1148
BsuRI GGCC 9 cut(s) 116, 242, 368, 426, 548, 670, 1450, 1704, 1815
BtgI CCRYGG 4 cut(s) 363, 665, 1732, 1953
BtgZI GCGATG 1 cut(s) 696
BtrI CACGTC 2 cut(s) 325, 1895
BtsCI GGATG 8 cut(s) 210, 889, 896, 1143, 1273, 1330, 1902, 1933
BtsI GCAGTG 2 cut(s) 1217, 1650
BtsIMutI CAGTG 2 cut(s) 1217, 1650
BveI ACCTGC 1 cut(s) 927
Cac8I GCNNGC 7 cut(s) 581, 1008, 1399, 1460, 1627, 1813, 2002
CciI TCATGA 1 cut(s) 66
CfoI GCGC 3 cut(s) 156, 310, 585
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1703, 1870, 1991
CsiI ACCWGGT 1 cut(s) 1885
Csp6I GTAC 4 cut(s) 520, 704, 817, 1045
CspAI ACCGGT 1 cut(s) 107
CviQI GTAC 4 cut(s) 520, 704, 817, 1045
DdeI CTNAG 3 cut(s) 834, 1880, 1986
DraI TTTAAA 2 cut(s) 1582, 1668
EaeI YGGCCR 5 cut(s) 366, 424, 546, 668, 1448
Eam1104I CTCTTC 4 cut(s) 668, 1529, 1823, 2018
EarI CTCTTC 4 cut(s) 668, 1529, 1823, 2018
EciI GGCGGA 5 cut(s) 160, 258, 285, 461, 627
Ecl136II GAGCTC 1 cut(s) 1522
Eco130I CCWWGG 3 cut(s) 363, 665, 1953
Eco24I GRGCYC 3 cut(s) 118, 659, 1524
Eco47I GGWCC 4 cut(s) 105, 360, 1870, 1991
Eco47III AGCGCT 1 cut(s) 584
Eco53kI GAGCTC 1 cut(s) 1522
Eco57I CTGAAG 2 cut(s) 1412, 1466
Eco91I GGTNACC 1 cut(s) 860
EcoICRI GAGCTC 1 cut(s) 1522
EcoO109I RGGNCCY 2 cut(s) 114, 1991
EcoO65I GGTNACC 1 cut(s) 860
EcoRII CCWGG 3 cut(s) 237, 1673, 1885
EcoT14I CCWWGG 3 cut(s) 363, 665, 1953
EcoT22I ATGCAT 2 cut(s) 1403, 1755
EcoT38I GRGCYC 3 cut(s) 118, 659, 1524
ErhI CCWWGG 3 cut(s) 363, 665, 1953
Esp3I CGTCTC 1 cut(s) 184
FalI AAGNNNNNCTT 4 cut(s) 1209, 1241, 1508, 1540
FaqI GGGAC 3 cut(s) 373, 448, 802
FauI CCCGC 3 cut(s) 252, 313, 455
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 1899
FokI GGATG 8 cut(s) 197, 896, 903, 1150, 1280, 1337, 1889, 1940
FriOI GRGCYC 3 cut(s) 118, 659, 1524
FspBI CTAG 1 cut(s) 1202
GlaI GCGC 3 cut(s) 155, 309, 584
GsaI CCCAGC 2 cut(s) 121, 1252
HaeII RGCGCY 1 cut(s) 586
HaeIII GGCC 9 cut(s) 116, 242, 368, 426, 548, 670, 1450, 1704, 1815
HapII CCGG 5 cut(s) 108, 602, 609, 779, 1144
HhaI GCGC 3 cut(s) 156, 310, 585
Hin6I GCGC 3 cut(s) 154, 308, 583
HinP1I GCGC 3 cut(s) 154, 308, 583
HincII GTYRAC 2 cut(s) 1300, 1851
HindII GTYRAC 2 cut(s) 1300, 1851
HinfI GANTC 6 cut(s) 605, 973, 1099, 1352, 1410, 1535
HpaI GTTAAC 1 cut(s) 1300
HpaII CCGG 5 cut(s) 108, 602, 609, 779, 1144
HphI GGTGA 3 cut(s) 70, 127, 854
Hpy166II GTNNAC 5 cut(s) 349, 1300, 1851, 1892, 1900
Hpy188I TCNGA 6 cut(s) 322, 741, 978, 1392, 1485, 1987
Hpy8I GTNNAC 5 cut(s) 349, 1300, 1851, 1892, 1900
Hpy99I CGWCG 2 cut(s) 322, 374
HpyAV CCTTC 8 cut(s) 91, 142, 177, 217, 267, 297, 1387, 1556
HpyCH4III ACNGT 7 cut(s) 178, 509, 528, 795, 1494, 1733, 1874
HpyCH4IV ACGT 2 cut(s) 324, 1894
HpyF10VI GCNNNNNNNGC 9 cut(s) 253, 277, 307, 589, 1003, 1016, 1025, 1083, 1222
HpyF3I CTNAG 3 cut(s) 834, 1880, 1986
HpySE526I ACGT 2 cut(s) 324, 1894
HspAI GCGC 3 cut(s) 154, 308, 583
Kpn2I TCCGGA 1 cut(s) 601
Ksp22I TGATCA 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 1300
LguI GCTCTTC 3 cut(s) 668, 1529, 2018
LmnI GCTCC 8 cut(s) 129, 420, 459, 958, 1146, 1207, 1430, 1519
Lsp1109I GCAGC 6 cut(s) 133, 909, 1031, 1181, 1295, 1820
LweI GCATC 6 cut(s) 1073, 1128, 1212, 1315, 1489, 1900
MabI ACCWGGT 1 cut(s) 1885
MaeI CTAG 1 cut(s) 1202
MaeII ACGT 2 cut(s) 324, 1894
MaeIII GTNAC 6 cut(s) 76, 452, 503, 860, 1103, 1438
MbiI CCGCTC 3 cut(s) 423, 462, 592
MfeI CAATTG 1 cut(s) 948
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 3 cut(s) 118, 659, 1524
MlsI TGGCCA 2 cut(s) 548, 1450
MluNI TGGCCA 2 cut(s) 548, 1450
MlyI GAGTC 3 cut(s) 1093, 1346, 1404
MmeI TCCRAC 2 cut(s) 300, 868
Mox20I TGGCCA 2 cut(s) 548, 1450
Mph1103I ATGCAT 2 cut(s) 1403, 1755
MroI TCCGGA 1 cut(s) 601
MscI TGGCCA 2 cut(s) 548, 1450
MslI CAYNNNNRTG 1 cut(s) 941
Msp20I TGGCCA 2 cut(s) 548, 1450
MspA1I CMGCKG 3 cut(s) 334, 1308, 1811
MspI CCGG 5 cut(s) 108, 602, 609, 779, 1144
MspR9I CCNGG 5 cut(s) 239, 780, 1145, 1675, 1887
MunI CAATTG 1 cut(s) 948
MvaI CCWGG 3 cut(s) 239, 1675, 1887
MvnI CGCG 3 cut(s) 154, 308, 681
MwoI GCNNNNNNNGC 9 cut(s) 253, 277, 307, 589, 1003, 1016, 1025, 1083, 1222
NciI CCSGG 2 cut(s) 780, 1145
NcoI CCATGG 3 cut(s) 363, 665, 1953
NlaIV GGNNCC 9 cut(s) 35, 115, 116, 243, 361, 633, 1692, 1705, 1992
NmuCI GTSAC 5 cut(s) 76, 452, 860, 1103, 1438
NsiI ATGCAT 2 cut(s) 1403, 1755
NspI RCATGY 3 cut(s) 1401, 1462, 1808
PaeI GCATGC 2 cut(s) 1401, 1462
PagI TCATGA 1 cut(s) 66
PciSI GCTCTTC 3 cut(s) 668, 1529, 2018
PcsI WCGNNNNNNNCGW 4 cut(s) 18, 384, 390, 513
PfeI GAWTC 3 cut(s) 605, 973, 1535
PflMI CCANNNNNTGG 2 cut(s) 666, 1959
PfoI TCCNGGA 1 cut(s) 778
PinAI ACCGGT 1 cut(s) 107
PleI GAGTC 3 cut(s) 1093, 1346, 1404
PpsI GAGTC 3 cut(s) 1093, 1346, 1404
PpuMI RGGWCCY 1 cut(s) 1991
PshBI ATTAAT 1 cut(s) 1092
PsiI TTATAA 1 cut(s) 1185
Psp124BI GAGCTC 1 cut(s) 1524
Psp5II RGGWCCY 1 cut(s) 1991
Psp6I CCWGG 3 cut(s) 237, 1673, 1885
PspEI GGTNACC 1 cut(s) 860
PspFI CCCAGC 2 cut(s) 117, 1248
PspGI CCWGG 3 cut(s) 237, 1673, 1885
PspN4I GGNNCC 9 cut(s) 35, 115, 116, 243, 361, 633, 1692, 1705, 1992
PspOMI GGGCCC 1 cut(s) 114
PspPI GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1703, 1870, 1991
PspPPI RGGWCCY 1 cut(s) 1991
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 2 cut(s) 1308, 1811
RsaI GTAC 4 cut(s) 521, 705, 818, 1046
RsaNI GTAC 4 cut(s) 520, 704, 817, 1045
RseI CAYNNNNRTG 1 cut(s) 941
SacI GAGCTC 1 cut(s) 1524
SapI GCTCTTC 3 cut(s) 668, 1529, 2018
Sau96I GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1703, 1870, 1991
ScaI AGTACT 1 cut(s) 818
SchI GAGTC 3 cut(s) 1093, 1346, 1404
ScrFI CCNGG 5 cut(s) 239, 780, 1145, 1675, 1887
SduI GDGCHC 3 cut(s) 118, 659, 1524
SexAI ACCWGGT 1 cut(s) 1885
SfaNI GCATC 6 cut(s) 1073, 1128, 1212, 1315, 1489, 1900
SfcI CTRYAG 1 cut(s) 2005
SinI GGWCC 4 cut(s) 105, 360, 1870, 1991
SmiI ATTTAAAT 1 cut(s) 1668
SmiMI CAYNNNNRTG 1 cut(s) 941
SmlI CTYRAG 4 cut(s) 992, 1124, 1354, 1862
SmoI CTYRAG 4 cut(s) 992, 1124, 1354, 1862
SphI GCATGC 2 cut(s) 1401, 1462
SspI AATATT 1 cut(s) 1544
SspMI CTAG 1 cut(s) 1202
SstI GAGCTC 1 cut(s) 1524
StyD4I CCNGG 5 cut(s) 237, 778, 1143, 1673, 1885
StyI CCWWGG 3 cut(s) 363, 665, 1953
SwaI ATTTAAAT 1 cut(s) 1668
TaaI ACNGT 7 cut(s) 178, 509, 528, 795, 1494, 1733, 1874
TaiI ACGT 2 cut(s) 327, 1897
TaqI TCGA 3 cut(s) 378, 393, 871
TatI WGTACW 2 cut(s) 519, 816
TfiI GAWTC 3 cut(s) 605, 973, 1535
TscAI CASTG 2 cut(s) 1217, 1650
TseFI GTSAC 5 cut(s) 76, 452, 860, 1103, 1438
TseI GCWGC 6 cut(s) 121, 922, 1019, 1169, 1308, 1808
Tsp45I GTSAC 5 cut(s) 76, 452, 860, 1103, 1438
TspDTI ATGAA 5 cut(s) 199, 1206, 1566, 1697, 2005
TspGWI ACGGA 4 cut(s) 64, 943, 1562, 1895
TspRI CASTG 2 cut(s) 1217, 1650
Van91I CCANNNNNTGG 2 cut(s) 666, 1959
VpaK11BI GGWCC 4 cut(s) 105, 360, 1870, 1991
VspI ATTAAT 1 cut(s) 1092
XapI RAATTY 1 cut(s) 1669
XceI RCATGY 3 cut(s) 1401, 1462, 1808
XcmI CCANNNNNNNNNTGG 1 cut(s) 1374
XmiI GTMKAC 1 cut(s) 1899
XspI CTAG 1 cut(s) 1202
ZrmI AGTACT 1 cut(s) 818
Zsp2I ATGCAT 2 cut(s) 1403, 1755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.