MD01G1146100.v1.1
ERF Family

HAD superfamily, subfamily IIIB (Acid phosphatase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005130
Physical Location & Seq
Reverse (-)
25583639 .. 25584470
832 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1146100.v1.1.491

Sequence Viewer

Length: 426 bp
ATGTTTATTCTGAGATTATATATGGAACTTCATGCTGGTGGTTGGCCTTTGATTCTGCTCTCAAGAAAACCGGAGGCGGAGCGAAATTCTTCCATGGAGGATCTTATATCTGCAGGATATAGAGCTTGGTCTTCATTGATTATGAGATCGGAAGATGAATTGCATATGGAGAGCCATGACTACTTCTCCAAACGAAGGGACGCGATGCAGAGAGAAGGCTTCCGCGCTATAGCCTCCGTAAGCAGTCACATGGATGCTCTAAGAAGTCCATTTTCAGGCGAGCGGATTTTCAAGCTTCCAAACCCAATTTTTTACAATTTTTCACATCAAATTGAGGACATACATACAGATGTGGGCGTCGCCGCATTGTCTAGAGCGAATGTGCTCTCCCGTATACACCCGAATTTAAATTCCTCTGCCGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

142

Amino Acids

16.15

Weight (kDa)

6.65

Isoelectric Point (pI)

56.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 4 - 105 3.7e-13 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 283
AccI GTMKAC 1 cut(s) 394
AccII CGCG 2 cut(s) 203, 225
AciI CCGC 4 cut(s) 77, 223, 283, 363
AclWI GGATC 1 cut(s) 108
AcsI RAATTY 3 cut(s) 85, 403, 409
AcyI GRCGYC 1 cut(s) 357
AfiI CCNNNNNNNGG 2 cut(s) 195, 275
AgsI TTSAA 1 cut(s) 292
AluBI AGCT 2 cut(s) 125, 295
AluI AGCT 2 cut(s) 125, 295
Alw21I GWGCWC 1 cut(s) 387
AlwI GGATC 1 cut(s) 108
AoxI GGCC 1 cut(s) 44
ApoI RAATTY 3 cut(s) 85, 403, 409
Asp700I GAANNNNTTC 1 cut(s) 88
AspLEI GCGC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 1 cut(s) 387
BceAI ACGGC 1 cut(s) 404
BfaI CTAG 1 cut(s) 372
BfmI CTRYAG 2 cut(s) 111, 228
BisI GCNGC 1 cut(s) 363
BlsI GCNGC 1 cut(s) 364
BmsI GCATC 2 cut(s) 195, 244
BpiI GAAGAC 1 cut(s) 123
BpuEI CTTGAG 1 cut(s) 46
BsaHI GRCGYC 1 cut(s) 357
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 275
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 259
BseLI CCNNNNNNNGG 2 cut(s) 195, 275
Bsh1236I CGCG 2 cut(s) 203, 225
BshFI GGCC 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 1 cut(s) 71
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 2 cut(s) 195, 275
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 1 cut(s) 46
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 2 cut(s) 100, 146
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 4 cut(s) 77, 223, 283, 363
BspANI GGCC 1 cut(s) 46
BspFNI CGCG 2 cut(s) 203, 225
BspMAI CTGCAG 1 cut(s) 115
BspPI GGATC 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 283
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 2 cut(s) 100, 146
BssNAI GTATAC 1 cut(s) 395
BssNI GRCGYC 1 cut(s) 357
BssT1I CCWWGG 1 cut(s) 93
Bst1107I GTATAC 1 cut(s) 395
BstACI GRCGYC 1 cut(s) 357
BstC8I GCNNGC 1 cut(s) 281
BstDEI CTNAG 2 cut(s) 11, 260
BstDSI CCRYGG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 259
BstFNI CGCG 2 cut(s) 203, 225
BstHHI GCGC 1 cut(s) 227
BstKTI GATC 2 cut(s) 103, 149
BstMBI GATC 2 cut(s) 100, 146
BstSFI CTRYAG 2 cut(s) 111, 228
BstUI CGCG 2 cut(s) 203, 225
BstV2I GAAGAC 1 cut(s) 123
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BstZ17I GTATAC 1 cut(s) 395
BsuRI GGCC 1 cut(s) 46
BtgI CCRYGG 1 cut(s) 93
BtgZI GCGATG 1 cut(s) 218
BtsCI GGATG 1 cut(s) 259
Cac8I GCNNGC 1 cut(s) 281
CfoI GCGC 1 cut(s) 227
CseI GACGC 2 cut(s) 209, 346
CviAII CATG 4 cut(s) 32, 94, 176, 250
CviJI RGCY 6 cut(s) 46, 125, 174, 219, 233, 295
CviKI_1 RGCY 6 cut(s) 46, 125, 174, 219, 233, 295
DdeI CTNAG 2 cut(s) 11, 260
DpnI GATC 2 cut(s) 102, 148
DpnII GATC 2 cut(s) 100, 146
DraI TTTAAA 1 cut(s) 408
EciI GGCGGA 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 93
EcoT14I CCWWGG 1 cut(s) 93
ErhI CCWWGG 1 cut(s) 93
FaeI CATG 4 cut(s) 35, 97, 179, 253
FaqI GGGAC 1 cut(s) 212
FatI CATG 4 cut(s) 31, 93, 175, 249
FauNDI CATATG 1 cut(s) 165
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 363
FokI GGATG 1 cut(s) 266
Fsp4HI GCNGC 1 cut(s) 363
FspBI CTAG 1 cut(s) 372
GlaI GCGC 1 cut(s) 226
GluI GCNGC 1 cut(s) 363
HaeIII GGCC 1 cut(s) 46
HapII CCGG 1 cut(s) 71
HgaI GACGC 2 cut(s) 209, 346
HhaI GCGC 1 cut(s) 227
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 4 cut(s) 35, 97, 179, 253
Hin6I GCGC 1 cut(s) 225
HinP1I GCGC 1 cut(s) 225
HindIII AAGCTT 1 cut(s) 293
HinfI GANTC 1 cut(s) 52
HpaII CCGG 1 cut(s) 71
Hpy166II GTNNAC 1 cut(s) 395
Hpy188I TCNGA 2 cut(s) 12, 151
Hpy188III TCNNGA 2 cut(s) 63, 372
Hpy8I GTNNAC 1 cut(s) 395
Hpy99I CGWCG 1 cut(s) 362
HpyAV CCTTC 2 cut(s) 189, 209
HpyCH4V TGCA 3 cut(s) 113, 163, 208
HpyF3I CTNAG 2 cut(s) 11, 260
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 4 cut(s) 35, 97, 179, 253
HspAI GCGC 1 cut(s) 225
Kzo9I GATC 2 cut(s) 100, 146
LmnI GCTCC 1 cut(s) 79
LpnPI CCDG 4 cut(s) 21, 84, 99, 261
LweI GCATC 2 cut(s) 195, 244
MaeI CTAG 1 cut(s) 372
MaeIII GTNAC 1 cut(s) 245
MalI GATC 2 cut(s) 102, 148
MbiI CCGCTC 1 cut(s) 283
MboI GATC 2 cut(s) 100, 146
MboII GAAGA 3 cut(s) 81, 123, 164
MflI RGATCY 1 cut(s) 100
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 7 cut(s) 85, 158, 306, 316, 330, 403, 409
MnlI CCTC 5 cut(s) 67, 91, 244, 328, 424
MroXI GAANNNNTTC 1 cut(s) 88
MseI TTAA 1 cut(s) 407
MslI CAYNNNNRTG 3 cut(s) 36, 252, 348
MspI CCGG 1 cut(s) 71
MvnI CGCG 2 cut(s) 203, 225
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 165
NdeII GATC 2 cut(s) 100, 146
NlaIII CATG 4 cut(s) 35, 97, 179, 253
NmuCI GTSAC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 88
PfeI GAWTC 1 cut(s) 52
PkrI GCNGC 1 cut(s) 364
PstI CTGCAG 1 cut(s) 115
PsuI RGATCY 1 cut(s) 100
RseI CAYNNNNRTG 3 cut(s) 36, 252, 348
SaqAI TTAA 1 cut(s) 407
SatI GCNGC 1 cut(s) 363
Sau3AI GATC 2 cut(s) 100, 146
SduI GDGCHC 1 cut(s) 387
SetI ASST 2 cut(s) 127, 297
SfaNI GCATC 2 cut(s) 195, 244
SfcI CTRYAG 2 cut(s) 111, 228
SmiI ATTTAAAT 1 cut(s) 408
SmiMI CAYNNNNRTG 3 cut(s) 36, 252, 348
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
Sse9I AATT 7 cut(s) 85, 158, 306, 316, 330, 403, 409
SsiI CCGC 4 cut(s) 77, 223, 283, 363
SspMI CTAG 1 cut(s) 372
StyI CCWWGG 1 cut(s) 93
SwaI ATTTAAAT 1 cut(s) 408
TasI AATT 7 cut(s) 85, 158, 306, 316, 330, 403, 409
TauI GCSGC 1 cut(s) 365
TfiI GAWTC 1 cut(s) 52
Tru1I TTAA 1 cut(s) 407
Tru9I TTAA 1 cut(s) 407
TseFI GTSAC 1 cut(s) 245
Tsp45I GTSAC 1 cut(s) 245
TspDTI ATGAA 3 cut(s) 20, 123, 171
TspGWI ACGGA 1 cut(s) 226
XapI RAATTY 3 cut(s) 85, 403, 409
XbaI TCTAGA 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 394
XmnI GAANNNNTTC 1 cut(s) 88
XspI CTAG 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.