MD01G1146200.v1.1
ERF Family

HAD superfamily, subfamily IIIB (Acid phosphatase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005130
Physical Location & Seq
Reverse (-)
25584472 .. 25586055
1584 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1146200.v1.1.491

Sequence Viewer

Length: 504 bp
ATGTCTGCCTATGGACGTCATATGGAGCGCGAGTACTCCGCCCAGAGTCTTTCGGGTGGAGGTTCAGAGAGGGCAAGCAGTTATGCCATGGAGACGGGGTTCTACATGTCGTCTTTTGCTACGGCAATCTTCATTGGTGCACTTGTAGCTGTTGGGGTCTTATTGATCACCTTGCTGATTGCACTGACTGTAATGCTGCAGTCTTGCGAAAGTAAGAGCCATGGAGTCGTTGAGACTCAGAAACCGAGTTTCGATTACAATCACTGCCAGATCTTCTCTCTGCATATGGAACTCAACGCCGTTGAAGCAGATCGCTTCCCTTCAATGTGCAGGGTTGTTGCCCTTCAGTACATCAAAGAAGGTCAATACGCAAGAGACTTAAATTCTACCGTGTCGATGATTCAGAATTATTTCAGCAGCATTACCCCAACACACGATGGTCTCGACGTGGTCTTGATCGACATAGATGACATTCTTTCTTCAAATCGTAGACTGCACCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

168

Amino Acids

18.54

Weight (kDa)

5.6

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 81 - 158 2.5e-07 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 19
AccI GTMKAC 1 cut(s) 490
AccII CGCG 1 cut(s) 30
AciI CCGC 1 cut(s) 39
AcsI RAATTY 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 1 cut(s) 16
AfaI GTAC 2 cut(s) 35, 350
AflIII ACRYGT 1 cut(s) 105
AgeI ACCGGT 1 cut(s) 498
AgsI TTSAA 3 cut(s) 305, 324, 483
AjiI CACGTC 1 cut(s) 448
AloI GAACNNNNNNTCC 2 cut(s) 83, 115
AluBI AGCT 1 cut(s) 149
AluI AGCT 1 cut(s) 149
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 4 cut(s) 86, 227, 369, 446
Alw44I GTGCAC 1 cut(s) 138
ApaLI GTGCAC 1 cut(s) 138
ApeKI GCWGC 2 cut(s) 196, 417
ApoI RAATTY 1 cut(s) 382
AsiGI ACCGGT 1 cut(s) 498
Asp700I GAANNNNTTC 1 cut(s) 410
AspLEI GCGC 1 cut(s) 30
AsuHPI GGTGA 1 cut(s) 160
BaeGI GKGCMC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 142
BbvI GCAGC 2 cut(s) 183, 429
BccI CCATC 1 cut(s) 431
BceAI ACGGC 2 cut(s) 138, 284
BclI TGATCA 1 cut(s) 165
BcoDI GTCTC 4 cut(s) 86, 227, 369, 446
BfmI CTRYAG 1 cut(s) 197
BglII AGATCT 1 cut(s) 270
BisI GCNGC 2 cut(s) 197, 418
BlsI GCNGC 2 cut(s) 198, 419
BmcAI AGTACT 1 cut(s) 35
BmgBI CACGTC 1 cut(s) 448
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 16
BsaI GGTCTC 1 cut(s) 446
BsaJI CCNNGG 2 cut(s) 87, 220
BsaWI WCCGGW 1 cut(s) 498
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 83, 113
Bse118I RCCGGY 1 cut(s) 498
Bse8I GATNNNNATC 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 87, 220
BseJI GATNNNNATC 1 cut(s) 258
BseMII CTCAG 1 cut(s) 251
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 2 cut(s) 183, 429
BsgI GTGCAG 2 cut(s) 349, 479
Bsh1236I CGCG 1 cut(s) 30
BshTI ACCGGT 1 cut(s) 498
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 1 cut(s) 499
BsmAI GTCTC 4 cut(s) 86, 227, 369, 446
BsmBI CGTCTC 1 cut(s) 86
Bso31I GGTCTC 1 cut(s) 446
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 4 cut(s) 165, 270, 310, 456
Bsp19I CCATGG 2 cut(s) 87, 220
BspACI CCGC 1 cut(s) 39
BspCNI CTCAG 1 cut(s) 250
BspFNI CGCG 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 201
BspTNI GGTCTC 1 cut(s) 446
BsrFI RCCGGY 1 cut(s) 498
BssAI RCCGGY 1 cut(s) 498
BssECI CCNNGG 2 cut(s) 87, 220
BssMI GATC 4 cut(s) 165, 270, 310, 456
BssNI GRCGYC 1 cut(s) 16
BssT1I CCWWGG 2 cut(s) 87, 220
Bst4CI ACNGT 2 cut(s) 190, 391
BstACI GRCGYC 1 cut(s) 16
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 1 cut(s) 237
BstDSI CCRYGG 2 cut(s) 87, 220
BstFNI CGCG 1 cut(s) 30
BstHHI GCGC 1 cut(s) 30
BstKTI GATC 4 cut(s) 168, 273, 313, 459
BstMAI GTCTC 4 cut(s) 86, 227, 369, 446
BstMBI GATC 4 cut(s) 165, 270, 310, 456
BstMWI GCNNNNNNNGC 2 cut(s) 146, 305
BstNSI RCATGY 1 cut(s) 109
BstSFI CTRYAG 1 cut(s) 197
BstSLI GKGCMC 1 cut(s) 142
BstUI CGCG 1 cut(s) 30
BstV1I GCAGC 2 cut(s) 183, 429
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
BtgI CCRYGG 2 cut(s) 87, 220
BtrI CACGTC 1 cut(s) 448
BtsI GCAGTG 1 cut(s) 262
BtsIMutI CAGTG 2 cut(s) 182, 262
Cac8I GCNNGC 1 cut(s) 76
CfoI GCGC 1 cut(s) 30
Cfr10I RCCGGY 1 cut(s) 498
Csp6I GTAC 2 cut(s) 34, 349
CspAI ACCGGT 1 cut(s) 498
CviAII CATG 3 cut(s) 88, 106, 221
CviJI RGCY 2 cut(s) 149, 219
CviKI_1 RGCY 2 cut(s) 149, 219
CviQI GTAC 2 cut(s) 34, 349
DdeI CTNAG 1 cut(s) 237
DpnI GATC 4 cut(s) 167, 272, 312, 458
DpnII GATC 4 cut(s) 165, 270, 310, 456
EciI GGCGGA 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 87, 220
Eco31I GGTCTC 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 329
EcoT14I CCWWGG 2 cut(s) 87, 220
ErhI CCWWGG 2 cut(s) 87, 220
Esp3I CGTCTC 1 cut(s) 86
FaeI CATG 3 cut(s) 91, 109, 224
FatI CATG 3 cut(s) 87, 105, 220
FauNDI CATATG 2 cut(s) 21, 285
FbaI TGATCA 1 cut(s) 165
FblI GTMKAC 1 cut(s) 490
Fnu4HI GCNGC 2 cut(s) 197, 418
Fsp4HI GCNGC 2 cut(s) 197, 418
GlaI GCGC 1 cut(s) 29
GluI GCNGC 2 cut(s) 197, 418
HapII CCGG 1 cut(s) 499
HhaI GCGC 1 cut(s) 30
Hin1I GRCGYC 1 cut(s) 16
Hin1II CATG 3 cut(s) 91, 109, 224
Hin6I GCGC 1 cut(s) 28
HinP1I GCGC 1 cut(s) 28
HinfI GANTC 4 cut(s) 46, 225, 235, 400
HpaII CCGG 1 cut(s) 499
HphI GGTGA 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 140, 491
Hpy188I TCNGA 3 cut(s) 67, 240, 405
Hpy188III TCNNGA 2 cut(s) 443, 454
Hpy8I GTNNAC 2 cut(s) 140, 491
Hpy99I CGWCG 1 cut(s) 449
HpyAV CCTTC 3 cut(s) 330, 353, 353
HpyCH4III ACNGT 2 cut(s) 190, 391
HpyCH4IV ACGT 2 cut(s) 16, 447
HpyCH4V TGCA 6 cut(s) 140, 182, 199, 283, 330, 496
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 305
HpyF3I CTNAG 1 cut(s) 237
HpySE526I ACGT 2 cut(s) 16, 447
Hsp92I GRCGYC 1 cut(s) 16
Hsp92II CATG 3 cut(s) 91, 109, 224
HspAI GCGC 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 165
Kzo9I GATC 4 cut(s) 165, 270, 310, 456
LmnI GCTCC 1 cut(s) 25
LpnPI CCDG 3 cut(s) 56, 281, 316
Lsp1109I GCAGC 2 cut(s) 183, 429
MaeII ACGT 2 cut(s) 16, 447
MalI GATC 4 cut(s) 167, 272, 312, 458
MboI GATC 4 cut(s) 165, 270, 310, 456
MboII GAAGA 3 cut(s) 121, 265, 471
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 2 cut(s) 382, 406
MlyI GAGTC 3 cut(s) 55, 229, 234
MnlI CCTC 2 cut(s) 53, 63
MroXI GAANNNNTTC 1 cut(s) 410
MseI TTAA 1 cut(s) 380
MspI CCGG 1 cut(s) 499
MvnI CGCG 1 cut(s) 30
MwoI GCNNNNNNNGC 2 cut(s) 146, 305
NcoI CCATGG 2 cut(s) 87, 220
NdeI CATATG 2 cut(s) 21, 285
NdeII GATC 4 cut(s) 165, 270, 310, 456
NlaIII CATG 3 cut(s) 91, 109, 224
NspI RCATGY 1 cut(s) 109
PciI ACATGT 1 cut(s) 105
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PdmI GAANNNNTTC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 400
PflFI GACNNNGTC 1 cut(s) 449
PinAI ACCGGT 1 cut(s) 498
PkrI GCNGC 2 cut(s) 198, 419
PleI GAGTC 3 cut(s) 54, 229, 233
PpsI GAGTC 3 cut(s) 54, 229, 233
PscI ACATGT 1 cut(s) 105
PstI CTGCAG 1 cut(s) 201
PsuI RGATCY 1 cut(s) 270
PsyI GACNNNGTC 1 cut(s) 449
RsaI GTAC 2 cut(s) 35, 350
RsaNI GTAC 2 cut(s) 34, 349
SaqAI TTAA 1 cut(s) 380
SatI GCNGC 2 cut(s) 197, 418
Sau3AI GATC 4 cut(s) 165, 270, 310, 456
ScaI AGTACT 1 cut(s) 35
SchI GAGTC 3 cut(s) 55, 229, 234
SduI GDGCHC 1 cut(s) 142
SetI ASST 6 cut(s) 19, 64, 151, 173, 364, 450
SfcI CTRYAG 1 cut(s) 197
Sse9I AATT 2 cut(s) 382, 406
SsiI CCGC 1 cut(s) 39
StyI CCWWGG 2 cut(s) 87, 220
TaaI ACNGT 2 cut(s) 190, 391
TaiI ACGT 2 cut(s) 19, 450
TaqI TCGA 4 cut(s) 252, 395, 444, 459
TasI AATT 2 cut(s) 382, 406
TatI WGTACW 2 cut(s) 33, 348
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 380
Tru9I TTAA 1 cut(s) 380
TscAI CASTG 2 cut(s) 189, 269
TseI GCWGC 2 cut(s) 196, 417
TspDTI ATGAA 1 cut(s) 121
TspRI CASTG 2 cut(s) 189, 269
Tth111I GACNNNGTC 1 cut(s) 449
VneI GTGCAC 1 cut(s) 138
XapI RAATTY 1 cut(s) 382
XceI RCATGY 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 490
XmnI GAANNNNTTC 1 cut(s) 410
ZraI GACGTC 1 cut(s) 17
ZrmI AGTACT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.