MD07G1213100.v1.1
ERF Family

HAD superfamily, subfamily IIIB (Acid phosphatase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005130
Physical Location & Seq
Reverse (-)
29085618 .. 29089717
4100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1213100.v1.1.491

Sequence Viewer

Length: 903 bp
ATGTCTGCCTATGGCCGTCATATGGAGCGGGAGTACTCCCCGGAGAGTCTCTCGAGCGAAGGTTCTGAAAGGGCAAGCAGTTATGCCATGGAGACAGGATTCTACATGTCGTCTTTTGCTACGACAATCTTCATTGGCGCACTTATAACTGTTGGGCTCTTATTAATCGCCTTGCTGATTGCACTGACTGTAATGCTGCAGTCTTGCGAAAATAGGAGTCATGGAATCGTTGAGACTCATAACCCGAGTTTCGATTACAATCACTGCCAGATCTTGTCTTTGCATATGGAACTTAACGGTGAAGCAGATCGCTTCCCTTCAATGTGCAGGGTTGTTGCCCTTCAGTACATCAAAGAAGGTCAATATGTAAGAGATTTAAATTCTACCATGTCAATGATTCAGAATTATTTCAGCAGCATTACCCCAGCACACGATGGTCTGGACGTGGTCTTGATCGACATAGATGACATTCGTTCTTCAAATCCTCGGCACATTAATTTGCCACTGCCCCAATATGATCAGTATGGTTGTGGTGATTGTGTGGAAGAGGCAAAGCATCTGAAGCACAGGTTTATTCTGAGATTATACATGGAGCTTCATGCTAGCGGTTGGCCCTTGATTCTGCTATCAAGAAAGCCGGAGGCGGAGCGGAATTCCTCCATAGAGGATCTTATATCTGCAGGGTTTAGAGCTTGGTCTACATTGACAATGAGATCGGAAGATGAATTGCATATGGAGAGCCGTGACTACTTCTCGAAACGAAGGGATGCAATGCAGAGAGAAGGCTTCCGCATACTAGCTTCCATTAGCAGTCACATGGATGCTCTAAGAGGTCCGTTTTCAGGAGAGCGGATTTTCAAGCTTCCAAACCCAATTTATTACAATTTTTCAACATCGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0010035 GO:0010038 GO:0042221 GO:0046686 GO:0050896
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Acid_phosphat_B

Protein Analysis

301

Amino Acids

34.25

Weight (kDa)

5.73

Isoelectric Point (pI)

53.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acid_phosphat_B PF03767 80 - 294 3.4e-25 HAD superfamily, subfamily IIIB (Acid phosphatase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 146
AccBSI CCGCTC 3 cut(s) 28, 649, 850
AccI GTMKAC 1 cut(s) 698
AciI CCGC 6 cut(s) 28, 606, 644, 649, 790, 850
AclWI GGATC 1 cut(s) 675
AcoI YGGCCR 1 cut(s) 13
AcsI RAATTY 2 cut(s) 379, 652
AcuI CTGAAG 2 cut(s) 326, 581
AfaI GTAC 2 cut(s) 35, 347
AfiI CCNNNNNNNGG 2 cut(s) 22, 842
AflIII ACRYGT 1 cut(s) 105
AgsI TTSAA 4 cut(s) 321, 480, 859, 891
AjiI CACGTC 1 cut(s) 445
AluBI AGCT 4 cut(s) 595, 692, 800, 862
AluI AGCT 4 cut(s) 595, 692, 800, 862
Alw26I GTCTC 3 cut(s) 53, 86, 227
AlwI GGATC 1 cut(s) 675
Ama87I CYCGRG 2 cut(s) 52, 244
AoxI GGCC 2 cut(s) 13, 611
ApeKI GCWGC 2 cut(s) 196, 414
ApoI RAATTY 2 cut(s) 379, 652
AseI ATTAAT 2 cut(s) 164, 495
Asp700I GAANNNNTTC 1 cut(s) 407
AspLEI GCGC 1 cut(s) 140
AspS9I GGNCC 2 cut(s) 612, 833
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 2 cut(s) 311, 545
AsuNHI GCTAGC 1 cut(s) 602
AvaI CYCGRG 2 cut(s) 52, 244
AvaII GGWCC 1 cut(s) 833
BanII GRGCYC 1 cut(s) 159
BbvI GCAGC 2 cut(s) 183, 426
BccI CCATC 1 cut(s) 428
BceAI ACGGC 1 cut(s) 726
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 1 cut(s) 41
BcoDI GTCTC 3 cut(s) 53, 86, 227
BfaI CTAG 2 cut(s) 603, 797
BfmI CTRYAG 2 cut(s) 197, 678
BglII AGATCT 1 cut(s) 270
BisI GCNGC 2 cut(s) 197, 415
BlsI GCNGC 2 cut(s) 198, 416
BmcAI AGTACT 1 cut(s) 35
Bme1390I CCNGG 1 cut(s) 41
Bme18I GGWCC 1 cut(s) 833
BmeT110I CYCGRG 2 cut(s) 52, 244
BmgBI CACGTC 1 cut(s) 445
BmgT120I GGNCC 2 cut(s) 612, 833
BmrFI CCNGG 1 cut(s) 41
BmsI GCATC 3 cut(s) 565, 757, 811
BmtI GCTAGC 1 cut(s) 606
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpuMI CCSGG 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 258
BsaJI CCNNGG 3 cut(s) 39, 87, 485
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 842
Bse3DI GCAATG 1 cut(s) 777
Bse8I GATNNNNATC 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 39, 87, 485
BseGI GGATG 2 cut(s) 772, 826
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 2 cut(s) 22, 842
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 1 cut(s) 569
BseXI GCAGC 2 cut(s) 183, 426
BseYI CCCAGC 1 cut(s) 424
BsgI GTGCAG 1 cut(s) 346
BshFI GGCC 2 cut(s) 15, 613
BsiHKCI CYCGRG 2 cut(s) 52, 244
BsiSI CCGG 2 cut(s) 41, 638
BslI CCNNNNNNNGG 2 cut(s) 22, 842
BsmAI GTCTC 3 cut(s) 53, 86, 227
BsnI GGCC 2 cut(s) 15, 613
BsoBI CYCGRG 2 cut(s) 52, 244
Bsp1286I GDGCHC 1 cut(s) 159
Bsp143I GATC 6 cut(s) 270, 307, 453, 517, 667, 713
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 6 cut(s) 28, 606, 644, 649, 790, 850
BspANI GGCC 2 cut(s) 15, 613
BspCNI CTCAG 1 cut(s) 570
BspMAI CTGCAG 2 cut(s) 201, 682
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 1 cut(s) 675
BsrBI CCGCTC 3 cut(s) 28, 649, 850
BsrDI GCAATG 1 cut(s) 777
BssECI CCNNGG 3 cut(s) 39, 87, 485
BssMI GATC 6 cut(s) 270, 307, 453, 517, 667, 713
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 3 cut(s) 151, 190, 299
Bst6I CTCTTC 1 cut(s) 540
BstC8I GCNNGC 2 cut(s) 76, 604
BstDEI CTNAG 2 cut(s) 578, 827
BstDSI CCRYGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 772, 826
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 6 cut(s) 273, 310, 456, 520, 670, 716
BstMAI GTCTC 3 cut(s) 53, 86, 227
BstMBI GATC 6 cut(s) 270, 307, 453, 517, 667, 713
BstMWI GCNNNNNNNGC 1 cut(s) 562
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 39
BstSFI CTRYAG 2 cut(s) 197, 678
BstV1I GCAGC 2 cut(s) 183, 426
BstX2I RGATCY 2 cut(s) 270, 667
BstYI RGATCY 2 cut(s) 270, 667
BsuRI GGCC 2 cut(s) 15, 613
BtgI CCRYGG 1 cut(s) 87
BtrI CACGTC 1 cut(s) 445
BtsCI GGATG 2 cut(s) 772, 826
BtsI GCAGTG 2 cut(s) 262, 503
BtsIMutI CAGTG 3 cut(s) 182, 262, 503
Cac8I GCNNGC 2 cut(s) 76, 604
CfoI GCGC 1 cut(s) 140
Cfr13I GGNCC 2 cut(s) 612, 833
Csp6I GTAC 2 cut(s) 34, 346
CviAII CATG 7 cut(s) 88, 106, 221, 388, 589, 599, 817
CviQI GTAC 2 cut(s) 34, 346
DdeI CTNAG 2 cut(s) 578, 827
DpnI GATC 6 cut(s) 272, 309, 455, 519, 669, 715
DpnII GATC 6 cut(s) 270, 307, 453, 517, 667, 713
DraI TTTAAA 1 cut(s) 378
EaeI YGGCCR 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
EciI GGCGGA 1 cut(s) 659
Eco130I CCWWGG 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 833
Eco57I CTGAAG 2 cut(s) 326, 581
Eco88I CYCGRG 2 cut(s) 52, 244
EcoRI GAATTC 1 cut(s) 652
EcoT14I CCWWGG 1 cut(s) 87
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 7 cut(s) 91, 109, 224, 391, 592, 602, 820
FatI CATG 7 cut(s) 87, 105, 220, 387, 588, 598, 816
FauI CCCGC 1 cut(s) 21
FauNDI CATATG 3 cut(s) 21, 285, 732
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 1 cut(s) 698
Fnu4HI GCNGC 2 cut(s) 197, 415
FokI GGATG 2 cut(s) 779, 833
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 2 cut(s) 197, 415
FspBI CTAG 2 cut(s) 603, 797
GlaI GCGC 1 cut(s) 139
GluI GCNGC 2 cut(s) 197, 415
GsaI CCCAGC 1 cut(s) 428
HaeIII GGCC 2 cut(s) 15, 613
HapII CCGG 2 cut(s) 41, 638
HhaI GCGC 1 cut(s) 140
Hin1II CATG 7 cut(s) 91, 109, 224, 391, 592, 602, 820
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 7 cut(s) 46, 99, 217, 225, 235, 397, 619
HpaII CCGG 2 cut(s) 41, 638
HphI GGTGA 2 cut(s) 311, 545
Hpy166II GTNNAC 1 cut(s) 699
Hpy188I TCNGA 5 cut(s) 67, 402, 561, 579, 718
Hpy188III TCNNGA 6 cut(s) 52, 440, 451, 630, 754, 843
Hpy8I GTNNAC 1 cut(s) 699
HpyAV CCTTC 6 cut(s) 53, 327, 350, 350, 756, 776
HpyCH4III ACNGT 3 cut(s) 151, 190, 299
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 8 cut(s) 182, 199, 283, 327, 680, 730, 770, 775
HpyF10VI GCNNNNNNNGC 1 cut(s) 562
HpyF3I CTNAG 2 cut(s) 578, 827
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 7 cut(s) 91, 109, 224, 391, 592, 602, 820
HspAI GCGC 1 cut(s) 138
Ksp22I TGATCA 1 cut(s) 517
Kzo9I GATC 6 cut(s) 270, 307, 453, 517, 667, 713
LmnI GCTCC 3 cut(s) 25, 592, 646
Lsp1109I GCAGC 2 cut(s) 183, 426
LweI GCATC 3 cut(s) 565, 757, 811
MaeI CTAG 2 cut(s) 603, 797
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 2 cut(s) 743, 812
MalI GATC 6 cut(s) 272, 309, 455, 519, 669, 715
MbiI CCGCTC 3 cut(s) 28, 649, 850
MboI GATC 6 cut(s) 270, 307, 453, 517, 667, 713
MboII GAAGA 4 cut(s) 121, 468, 557, 731
MflI RGATCY 2 cut(s) 270, 667
MhlI GDGCHC 1 cut(s) 159
MluCI AATT 8 cut(s) 379, 403, 496, 652, 725, 873, 883, 898
MlyI GAGTC 3 cut(s) 55, 226, 229
MnlI CCTC 6 cut(s) 495, 541, 634, 658, 667, 824
MroXI GAANNNNTTC 1 cut(s) 407
MseI TTAA 4 cut(s) 164, 294, 377, 495
MslI CAYNNNNRTG 2 cut(s) 392, 819
MspI CCGG 2 cut(s) 41, 638
MspR9I CCNGG 1 cut(s) 41
MwoI GCNNNNNNNGC 1 cut(s) 562
NciI CCSGG 1 cut(s) 41
NcoI CCATGG 1 cut(s) 87
NdeI CATATG 3 cut(s) 21, 285, 732
NdeII GATC 6 cut(s) 270, 307, 453, 517, 667, 713
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 7 cut(s) 91, 109, 224, 391, 592, 602, 820
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 2 cut(s) 743, 812
NspI RCATGY 1 cut(s) 109
PaeR7I CTCGAG 1 cut(s) 52
PciI ACATGT 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 4 cut(s) 99, 225, 397, 619
PflFI GACNNNGTC 1 cut(s) 446
PkrI GCNGC 2 cut(s) 198, 416
PleI GAGTC 3 cut(s) 54, 225, 229
PpsI GAGTC 3 cut(s) 54, 225, 229
PscI ACATGT 1 cut(s) 105
PshBI ATTAAT 2 cut(s) 164, 495
PsiI TTATAA 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 424
PspPI GGNCC 2 cut(s) 612, 833
PstI CTGCAG 2 cut(s) 201, 682
PsuI RGATCY 2 cut(s) 270, 667
PsyI GACNNNGTC 1 cut(s) 446
RsaI GTAC 2 cut(s) 35, 347
RsaNI GTAC 2 cut(s) 34, 346
RseI CAYNNNNRTG 2 cut(s) 392, 819
SaqAI TTAA 4 cut(s) 164, 294, 377, 495
SatI GCNGC 2 cut(s) 197, 415
Sau3AI GATC 6 cut(s) 270, 307, 453, 517, 667, 713
Sau96I GGNCC 2 cut(s) 612, 833
ScaI AGTACT 1 cut(s) 35
SchI GAGTC 3 cut(s) 55, 226, 229
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 159
SetI ASST 9 cut(s) 64, 361, 447, 572, 597, 694, 802, 835, 864
SfaNI GCATC 3 cut(s) 565, 757, 811
SfcI CTRYAG 2 cut(s) 197, 678
Sfr274I CTCGAG 1 cut(s) 52
SinI GGWCC 1 cut(s) 833
SlaI CTCGAG 1 cut(s) 52
SmiI ATTTAAAT 1 cut(s) 378
SmiMI CAYNNNNRTG 2 cut(s) 392, 819
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 8 cut(s) 379, 403, 496, 652, 725, 873, 883, 898
SsiI CCGC 6 cut(s) 28, 606, 644, 649, 790, 850
SspMI CTAG 2 cut(s) 603, 797
StyD4I CCNGG 1 cut(s) 39
StyI CCWWGG 1 cut(s) 87
SwaI ATTTAAAT 1 cut(s) 378
TaaI ACNGT 3 cut(s) 151, 190, 299
TaiI ACGT 1 cut(s) 447
TaqI TCGA 5 cut(s) 53, 252, 456, 755, 896
TasI AATT 8 cut(s) 379, 403, 496, 652, 725, 873, 883, 898
TatI WGTACW 2 cut(s) 33, 345
TfiI GAWTC 4 cut(s) 99, 225, 397, 619
Tru1I TTAA 4 cut(s) 164, 294, 377, 495
Tru9I TTAA 4 cut(s) 164, 294, 377, 495
TscAI CASTG 3 cut(s) 189, 269, 510
TseFI GTSAC 2 cut(s) 743, 812
TseI GCWGC 2 cut(s) 196, 414
Tsp45I GTSAC 2 cut(s) 743, 812
TspDTI ATGAA 3 cut(s) 121, 587, 738
TspGWI ACGGA 1 cut(s) 825
TspRI CASTG 3 cut(s) 189, 269, 510
Tth111I GACNNNGTC 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 833
VspI ATTAAT 2 cut(s) 164, 495
XapI RAATTY 2 cut(s) 379, 652
XceI RCATGY 1 cut(s) 109
XhoI CTCGAG 1 cut(s) 52
XmiI GTMKAC 1 cut(s) 698
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 2 cut(s) 603, 797
ZrmI AGTACT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.