Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0016615

Physical Location & Seq

Genomic Coordinates Reverse (-)

25711486 .. 25713983

Gene Structure 2498 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1148100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 858 bp

ATGGCGGCGATCGAGCACACTGCGTTGCAGTTCTCCACGTCTCCGTCGTCGAGCTTGTCGGCGAAGGTTCATCCTCTCGTCATCTTTAACATCTGCGATTGCTACGTCAGGCGTCCCGACCAGTCGGAGCGAGTCATCGGCACGCTCCTCGGCTCCGTCCTACCTGACGGAACTGTCGACATCAGAAACTCCTACGCTGTTCCTCATATGAGCGCCGAGCAGGTTGCTTTAGATATTGAGTACCATCACAATATGTTGATATCCCACCAGAAGGTGAATCCCAAGGAAGTCATCGTTGGATGGTATTCAACTGGTCTTGGAGTTACTGGTGGTAGTGCACTGTTTCATGAATTCTATTCTAGAGAAGCTCCCAACCCCGTTCATTTGACTGTTGATACTGGATTTGTTAATGGAGTGGGTGCCATAAAAGCTTATGTTTCAGTGAATTTGTCTCTTGGAGATCGGCAACTTGCTGCGCAGTTTCAAGAAATTCCTCTTGATCTTCGCATGGTTGAAGCTGAGCGAGTTGGATATGATATTTTAAAGACCCCAATGGTTGACAAACTCCCAACTGATCTTGAAGGAATGGAAGCCTCAATGGAGCGGCTTCTAGCTTTAATTGATGATGTTTACAAATATGTTGACAATGTTGTGGAAGGCCGTGTTGAACAAGATAACACTATAGGGAGATTTCTTTTTGATACGATTGCATCTCTTCCGAAACTGTCTCCATCAGCTTTTGATAAGCTTATCAATGACAGCCTGCAGGACAACTTGCTCTTACTGTACTTGTCAAGCATCACAAGGACACAGCTCACCTTAGCCGAAAAATTGAACACGGCTGCTCAGGTTCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005852 GO:0006412 GO:0006413 GO:0006518 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009743 GO:0009744 GO:0009790 GO:0009791 GO:0009793 GO:0009846 GO:0009856 GO:0009987 GO:0010033 GO:0010154 GO:0010467 GO:0016020 GO:0019538 GO:0022414 GO:0031369 GO:0032501 GO:0032502 GO:0032991 GO:0034285 GO:0034641 GO:0034645 GO:0042221 GO:0043043 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051704 GO:0061458 GO:0071541 GO:0071704 GO:1901564 GO:1901566 GO:1901576 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

JAB MitMem_reg

Protein Analysis

286

Amino Acids

31.42

Weight (kDa)

4.98

Isoelectric Point (pI)

34.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
JAB	PF01398	17 - 125	1.3e-24	JAB1/Mov34/MPN/PAD-1 ubiquitin protease
MitMem_reg	PF13012	172 - 279	3.9e-30	Maintenance of mitochondrial structure and function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016615)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39990
fragaria_vesca	FvH4_7g24110
malus_domestica	MD01G1148100.v1.1 MD07G1215300.v1.1
prunus_persica	Prupe.2G247200_v2.0.a1
pyrus_communis	pycom01g16690 pycom07g19760
rosa_chinensis	RchiOBHm_Chr1g0369531
rosa_laevigata	RLG00000027121
rosa_roxburghii	Rroxscaffold_4G00287550
rosa_rugosa	Rorug01G0351100
rosa_samantha	Rh1BG322900 Rh1DG353700
rosa_wichuraiana	Rw1G031650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	173
Acc16I	TGCGCA	1 cut(s)	477
Acc36I	ACCTGC	1 cut(s)	211
AccB1I	GGYRCC	1 cut(s)	419
AccBSI	CCGCTC	1 cut(s)	604
AccI	GTMKAC	1 cut(s)	177
AciI	CCGC	2 cut(s)	5, 604
AcsI	RAATTY	3 cut(s)	350, 445, 489
AcyI	GRCGYC	1 cut(s)	112
AfaI	GTAC	2 cut(s)	242, 788
AfiI	CCNNNNNNNGG	1 cut(s)	271
AgsI	TTSAA	6 cut(s)	309, 485, 515, 581, 668, 835
AjiI	CACGTC	1 cut(s)	39
AjuI	GAANNNNNNNTTGG	2 cut(s)	279, 311
AluBI	AGCT	8 cut(s)	54, 368, 431, 518, 614, 737, 748, 814
AluI	AGCT	8 cut(s)	54, 368, 431, 518, 614, 737, 748, 814
Alw21I	GWGCWC	2 cut(s)	18, 340
Alw26I	GTCTC	3 cut(s)	45, 456, 732
Alw44I	GTGCAC	1 cut(s)	336
AlwNI	CAGNNNCTG	1 cut(s)	853
AoxI	GGCC	1 cut(s)	658
ApaLI	GTGCAC	1 cut(s)	336
ApeKI	GCWGC	2 cut(s)	473, 842
ApoI	RAATTY	3 cut(s)	350, 445, 489
AspLEI	GCGC	2 cut(s)	215, 478
AsuHPI	GGTGA	2 cut(s)	286, 808
BaeGI	GKGCMC	1 cut(s)	340
BanI	GGYRCC	1 cut(s)	419
Bbv12I	GWGCWC	2 cut(s)	18, 340
BbvI	GCAGC	2 cut(s)	460, 829
BccI	CCATC	3 cut(s)	252, 294, 739
BceAI	ACGGC	2 cut(s)	645, 855
BcgI	CGANNNNNNTGC	5 cut(s)	36, 130, 164, 206, 240
BcoDI	GTCTC	3 cut(s)	45, 456, 732
BfaI	CTAG	2 cut(s)	360, 611
BfmI	CTRYAG	2 cut(s)	681, 764
BfoI	RGCGCY	1 cut(s)	216
BfuAI	ACCTGC	1 cut(s)	211
BisI	GCNGC	4 cut(s)	6, 474, 605, 843
BlpI	GCTNAGC	1 cut(s)	519
BlsI	GCNGC	4 cut(s)	7, 475, 606, 844
BmgBI	CACGTC	1 cut(s)	39
BmiI	GGNNCC	2 cut(s)	154, 421
BmsI	GCATC	2 cut(s)	719, 807
Bpu10I	CCTNAGC	2 cut(s)	820, 846
Bpu1102I	GCTNAGC	1 cut(s)	519
BsaHI	GRCGYC	1 cut(s)	112
BsaJI	CCNNGG	2 cut(s)	148, 282
Bsc4I	CCNNNNNNNGG	1 cut(s)	271
Bse1I	ACTGG	4 cut(s)	121, 316, 331, 403
BseDI	CCNNGG	2 cut(s)	148, 282
BseGI	GGATG	2 cut(s)	70, 305
BseLI	CCNNNNNNNGG	1 cut(s)	271
BseMII	CTCAG	1 cut(s)	510
BseNI	ACTGG	4 cut(s)	121, 316, 331, 403
BseRI	GAGGAG	1 cut(s)	137
BseSI	GKGCMC	1 cut(s)	340
BseXI	GCAGC	2 cut(s)	460, 829
Bsh1285I	CGRYCG	1 cut(s)	12
BshFI	GGCC	1 cut(s)	660
BshNI	GGYRCC	1 cut(s)	419
BsiEI	CGRYCG	1 cut(s)	12
BsiHKAI	GWGCWC	2 cut(s)	18, 340
BslFI	GGGAC	1 cut(s)	99
BslI	CCNNNNNNNGG	1 cut(s)	271
BsmAI	GTCTC	3 cut(s)	45, 456, 732
BsmBI	CGTCTC	1 cut(s)	45
BsmFI	GGGAC	1 cut(s)	99
BsnI	GGCC	1 cut(s)	660
Bsp1286I	GDGCHC	2 cut(s)	18, 340
Bsp143I	GATC	4 cut(s)	9, 460, 499, 574
Bsp1720I	GCTNAGC	1 cut(s)	519
BspACI	CCGC	2 cut(s)	5, 604
BspANI	GGCC	1 cut(s)	660
BspCNI	CTCAG	1 cut(s)	511
BspHI	TCATGA	1 cut(s)	346
BspLI	GGNNCC	2 cut(s)	154, 421
BspMAI	CTGCAG	1 cut(s)	768
BspMI	ACCTGC	1 cut(s)	211
BspT107I	GGYRCC	1 cut(s)	419
BsrBI	CCGCTC	1 cut(s)	604
BsrI	ACTGG	4 cut(s)	121, 316, 331, 403
BssECI	CCNNGG	2 cut(s)	148, 282
BssMI	GATC	4 cut(s)	9, 460, 499, 574
BssNI	GRCGYC	1 cut(s)	112
BssT1I	CCWWGG	1 cut(s)	282
Bst4CI	ACNGT	5 cut(s)	175, 342, 391, 726, 786
Bst6I	CTCTTC	1 cut(s)	720
BstACI	GRCGYC	1 cut(s)	112
BstC8I	GCNNGC	2 cut(s)	143, 764
BstDEI	CTNAG	3 cut(s)	519, 820, 846
BstF5I	GGATG	2 cut(s)	70, 305
BstH2I	RGCGCY	1 cut(s)	216
BstHHI	GCGC	2 cut(s)	215, 478
BstKTI	GATC	4 cut(s)	12, 463, 502, 577
BstMAI	GTCTC	3 cut(s)	45, 456, 732
BstMBI	GATC	4 cut(s)	9, 460, 499, 574
BstMCI	CGRYCG	1 cut(s)	12
BstMWI	GCNNNNNNNGC	1 cut(s)	428
BstSFI	CTRYAG	2 cut(s)	681, 764
BstSLI	GKGCMC	1 cut(s)	340
BstV1I	GCAGC	2 cut(s)	460, 829
BsuRI	GGCC	1 cut(s)	660
BtrI	CACGTC	1 cut(s)	39
BtsCI	GGATG	2 cut(s)	70, 305
BtsI	GCAGTG	1 cut(s)	18
BtsIMutI	CAGTG	3 cut(s)	18, 338, 447
BveI	ACCTGC	1 cut(s)	211
Cac8I	GCNNGC	2 cut(s)	143, 764
CaiI	CAGNNNCTG	1 cut(s)	853
CciI	TCATGA	1 cut(s)	346
CfoI	GCGC	2 cut(s)	215, 478
CseI	GACGC	1 cut(s)	101
Csp6I	GTAC	2 cut(s)	241, 787
CviAII	CATG	2 cut(s)	347, 508
CviQI	GTAC	2 cut(s)	241, 787
DdeI	CTNAG	3 cut(s)	519, 820, 846
DpnI	GATC	4 cut(s)	11, 462, 501, 576
DpnII	GATC	4 cut(s)	9, 460, 499, 574
DraI	TTTAAA	1 cut(s)	543
DrdI	GACNNNNNNGTC	1 cut(s)	173
DseDI	GACNNNNNNGTC	1 cut(s)	173
Eam1104I	CTCTTC	1 cut(s)	720
EarI	CTCTTC	1 cut(s)	720
Eco130I	CCWWGG	1 cut(s)	282
Eco32I	GATATC	1 cut(s)	261
EcoRI	GAATTC	1 cut(s)	350
EcoRV	GATATC	1 cut(s)	261
EcoT14I	CCWWGG	1 cut(s)	282
ErhI	CCWWGG	1 cut(s)	282
Esp3I	CGTCTC	1 cut(s)	45
FaeI	CATG	2 cut(s)	350, 511
FaqI	GGGAC	1 cut(s)	99
FatI	CATG	2 cut(s)	346, 507
FauNDI	CATATG	1 cut(s)	207
FblI	GTMKAC	1 cut(s)	177
Fnu4HI	GCNGC	4 cut(s)	6, 474, 605, 843
FokI	GGATG	2 cut(s)	57, 312
Fsp4HI	GCNGC	4 cut(s)	6, 474, 605, 843
FspBI	CTAG	2 cut(s)	360, 611
FspI	TGCGCA	1 cut(s)	477
GlaI	GCGC	2 cut(s)	214, 477
GluI	GCNGC	4 cut(s)	6, 474, 605, 843
HaeII	RGCGCY	1 cut(s)	216
HaeIII	GGCC	1 cut(s)	660
HgaI	GACGC	1 cut(s)	101
HhaI	GCGC	2 cut(s)	215, 478
Hin1I	GRCGYC	1 cut(s)	112
Hin1II	CATG	2 cut(s)	350, 511
Hin6I	GCGC	2 cut(s)	213, 476
HinP1I	GCGC	2 cut(s)	213, 476
HincII	GTYRAC	3 cut(s)	178, 559, 643
HindII	GTYRAC	3 cut(s)	178, 559, 643
HindIII	AAGCTT	2 cut(s)	429, 746
HinfI	GANTC	2 cut(s)	132, 277
HphI	GGTGA	2 cut(s)	286, 808
Hpy166II	GTNNAC	5 cut(s)	178, 338, 559, 631, 643
Hpy188I	TCNGA	3 cut(s)	127, 185, 720
Hpy188III	TCNNGA	6 cut(s)	116, 347, 360, 485, 497, 578
Hpy8I	GTNNAC	5 cut(s)	178, 338, 559, 631, 643
Hpy99I	CGWCG	2 cut(s)	49, 52
HpyAV	CCTTC	4 cut(s)	58, 265, 575, 650
HpyCH4III	ACNGT	5 cut(s)	175, 342, 391, 726, 786
HpyCH4IV	ACGT	2 cut(s)	38, 105
HpyCH4V	TGCA	4 cut(s)	28, 338, 710, 766
HpyF10VI	GCNNNNNNNGC	1 cut(s)	428
HpyF3I	CTNAG	3 cut(s)	519, 820, 846
HpySE526I	ACGT	2 cut(s)	38, 105
Hsp92I	GRCGYC	1 cut(s)	112
Hsp92II	CATG	2 cut(s)	350, 511
HspAI	GCGC	2 cut(s)	213, 476
Kzo9I	GATC	4 cut(s)	9, 460, 499, 574
LmnI	GCTCC	5 cut(s)	127, 150, 158, 373, 601
Lsp1109I	GCAGC	2 cut(s)	460, 829
LweI	GCATC	2 cut(s)	719, 807
MaeI	CTAG	2 cut(s)	360, 611
MaeII	ACGT	2 cut(s)	38, 105
MaeIII	GTNAC	1 cut(s)	322
MalI	GATC	4 cut(s)	11, 462, 501, 576
MbiI	CCGCTC	1 cut(s)	604
MboI	GATC	4 cut(s)	9, 460, 499, 574
MboII	GAAGA	2 cut(s)	494, 707
MhlI	GDGCHC	2 cut(s)	18, 340
MluCI	AATT	5 cut(s)	350, 445, 489, 618, 830
MlyI	GAGTC	1 cut(s)	141
MmeI	TCCRAC	3 cut(s)	105, 277, 508
MnlI	CCTC	5 cut(s)	84, 158, 213, 504, 604
MseI	TTAA	4 cut(s)	87, 408, 542, 617
MwoI	GCNNNNNNNGC	1 cut(s)	428
NdeI	CATATG	1 cut(s)	207
NdeII	GATC	4 cut(s)	9, 460, 499, 574
NlaIII	CATG	2 cut(s)	350, 511
NlaIV	GGNNCC	2 cut(s)	154, 421
NmeAIII	GCCGAG	2 cut(s)	129, 241
NsbI	TGCGCA	1 cut(s)	477
PagI	TCATGA	1 cut(s)	346
PcsI	WCGNNNNNNNCGW	2 cut(s)	44, 174
PfeI	GAWTC	1 cut(s)	277
PkrI	GCNGC	4 cut(s)	7, 475, 606, 844
Ple19I	CGATCG	1 cut(s)	12
PleI	GAGTC	1 cut(s)	140
PpsI	GAGTC	1 cut(s)	140
PspN4I	GGNNCC	2 cut(s)	154, 421
PstI	CTGCAG	1 cut(s)	768
PstNI	CAGNNNCTG	1 cut(s)	853
PvuI	CGATCG	1 cut(s)	12
RsaI	GTAC	2 cut(s)	242, 788
RsaNI	GTAC	2 cut(s)	241, 787
SalI	GTCGAC	1 cut(s)	176
SaqAI	TTAA	4 cut(s)	87, 408, 542, 617
SatI	GCNGC	4 cut(s)	6, 474, 605, 843
Sau3AI	GATC	4 cut(s)	9, 460, 499, 574
SbfI	CCTGCAGG	1 cut(s)	768
SchI	GAGTC	1 cut(s)	141
SdaI	CCTGCAGG	1 cut(s)	768
SduI	GDGCHC	2 cut(s)	18, 340
SfaNI	GCATC	2 cut(s)	719, 807
SfcI	CTRYAG	2 cut(s)	681, 764
Sse8387I	CCTGCAGG	1 cut(s)	768
Sse9I	AATT	5 cut(s)	350, 445, 489, 618, 830
SsiI	CCGC	2 cut(s)	5, 604
SspMI	CTAG	2 cut(s)	360, 611
StyI	CCWWGG	1 cut(s)	282
TaaI	ACNGT	5 cut(s)	175, 342, 391, 726, 786
TaiI	ACGT	2 cut(s)	41, 108
TaqI	TCGA	3 cut(s)	12, 50, 177
TasI	AATT	5 cut(s)	350, 445, 489, 618, 830
TatI	WGTACW	1 cut(s)	786
TauI	GCSGC	2 cut(s)	8, 607
TfiI	GAWTC	1 cut(s)	277
Tru1I	TTAA	4 cut(s)	87, 408, 542, 617
Tru9I	TTAA	4 cut(s)	87, 408, 542, 617
TscAI	CASTG	3 cut(s)	25, 345, 447
TseI	GCWGC	2 cut(s)	473, 842
TspDTI	ATGAA	4 cut(s)	59, 335, 363, 371
TspGWI	ACGGA	3 cut(s)	33, 145, 183
TspRI	CASTG	3 cut(s)	25, 345, 447
VneI	GTGCAC	1 cut(s)	336
XapI	RAATTY	3 cut(s)	350, 445, 489
XbaI	TCTAGA	1 cut(s)	359
XmiI	GTMKAC	1 cut(s)	177
XspI	CTAG	2 cut(s)	360, 611

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.