MD01G1149100.v1.1

Auxin transporter-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001953
Physical Location & Seq
Forward (+)
25776734 .. 25780967
4234 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1149100.v1.1.491

Sequence Viewer

Length: 1491 bp
ATGCTCTGTGGTCTTTCTGCAACTCTTTTCCTTCTCAGATCATTTAATCCGTCAGCAATGGCAGCACAGAAGCAATCAGAGGAAGCCATAACCTTCAACCAGACTGTTGACAGGGAGGAAGAAGAAGAACACGCAGCAGATCATAACTCTGGTTTTAACGTCAGAAACTTCCTCTGGCATGGCGGATCCGTCTACGACGCCTGGTTCAGCTGTGCATCGAACCAGGTTGCTCAGGTTCTGCTGACTTTGCCCTACTCTTTCTCCCAACTGGGAATGCTCTCGGGAATCATATTCCAGGTCTTCTACGGAATCATGGGGAGCTGGACCGCTTATCTGATCAGCATTCTCTACGTCGAATATCGAAGCCGAAAAGAAAAGGAAAATGTCAGCTTCAAGAACCATGTCATCCAGTGGTTCGAAGTGTTGGATGGTTTGCTGGGTCCTTACTGGAAAGCCGTTGGATTGGCCTTCAACTGCACTTTCCTCCTCTTTGGATCTGTCATCCAGCTTATTGGTTGTGCAAGCAACATATACTACATAAATGACCATCTGGACAAGAGGACATGGACCTACATATTTGGGGCATGTTGTGCCACCACAGTCTTCATCCCTTCCTTCCACAACTACAGAATATGGTCCTTTTTAGGGCTTGGGATGACCACCTACACTGCCTGGTACATGACCATTGCAGCCATTGTTCATGGCCAGGTTGAAGGTGTGAAACACTCAGGACCAGATAAACTGGTTTTGTATTTCACCGGCGCCACCAACATTCTCTACACTTTCGGCGGACACGCCGTCACTGTGGAAATCATGCATGCAATGTGGAAGCCTCAGAAGTTTAAGTATGTCTATCTGTTTGCTACCCTTTATGTGTTCACTCTAACCCTTCCGTCTGCAACTGCTGTGTACTGGGCGTTTGGCGACCAGCTCCTCACACACTCGAATGCCTTCTCGCTCCTTCCCCGGACTAAGTGGCGCGATGCGGGTGTCACATTGATGCTCATTCACCAGTTCATCACATTCGGGTTTGCTTGTACGCCATTGTATTTCGTGTGGGAGAAAGTAATTGGAATGCATGACACCAAGAGCATATGTTTGAGGGCAATTGCCAGGTTGCCTGTGGTGATACCTATTTGGTTCTTGGCCATCATATTTCCTTTCTTCGGTCCCATTAATTCAGCCGTTGGCGCTCTTTTGGTCAGCTTCACCGTCTACATCATACCCGCCTTAGCTCATATGCTCACCTTCAAATCCGCCTCTGCTCGCCAGAATGCGGCAGAGAAGCTTCCGTTCTTCCTCCCGAGCTGGACAGGCATGTACGTGGTGAACGCATTCATCGTGGTGTGGGTTCTCGTAGTCGGGTTCGGGTTCGGAGGGTGGGCTAGCGTGACCAACTTCATCAGGCAAGTTGACACTTTTGGGCTGTTTGCAAAGTGCTACCAGTGCCCTCCCAAAGTCCCAGCCTCCCTACCACCACCACATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

497

Amino Acids

55.89

Weight (kDa)

8.56

Isoelectric Point (pI)

31.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 60 - 453 1.1e-84 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 761
AccI GTMKAC 2 cut(s) 192, 1215
AccII CGCG 1 cut(s) 981
AciI CCGC 7 cut(s) 183, 327, 789, 986, 1227, 1257, 1277
AclWI GGATC 3 cut(s) 180, 193, 502
AcoI YGGCCR 2 cut(s) 703, 1146
AcyI GRCGYC 2 cut(s) 198, 762
AfaI GTAC 4 cut(s) 677, 911, 1039, 1322
AfiI CCNNNNNNNGG 3 cut(s) 645, 1166, 1276
AgsI TTSAA 5 cut(s) 97, 394, 472, 713, 1252
AhdI GACNNNNNGTC 1 cut(s) 797
AjnI CCWGG 6 cut(s) 200, 222, 294, 671, 705, 1112
AluBI AGCT 9 cut(s) 210, 321, 390, 508, 931, 1206, 1235, 1288, 1308
AluI AGCT 9 cut(s) 210, 321, 390, 508, 931, 1206, 1235, 1288, 1308
AlwI GGATC 3 cut(s) 180, 193, 502
AlwNI CAGNNNCTG 1 cut(s) 238
Ama87I CYCGRG 2 cut(s) 280, 1303
AoxI GGCC 3 cut(s) 465, 703, 1146
ApeKI GCWGC 3 cut(s) 62, 134, 689
AseI ATTAAT 1 cut(s) 1176
Asp700I GAANNNNTTC 2 cut(s) 950, 1334
AspLEI GCGC 3 cut(s) 764, 981, 1193
AspS9I GGNCC 6 cut(s) 324, 440, 567, 636, 731, 1169
AsuC2I CCSGG 1 cut(s) 967
AsuHPI GGTGA 6 cut(s) 748, 1001, 1138, 1201, 1237, 1339
AsuII TTCGAA 1 cut(s) 417
AsuNHI GCTAGC 1 cut(s) 1385
AvaI CYCGRG 2 cut(s) 280, 1303
AvaII GGWCC 6 cut(s) 324, 440, 567, 636, 731, 1169
BaeGI GKGCMC 1 cut(s) 1451
BalI TGGCCA 2 cut(s) 705, 1148
BamHI GGATCC 1 cut(s) 185
BanI GGYRCC 1 cut(s) 761
BbsI GAAGAC 2 cut(s) 292, 595
BbvI GCAGC 3 cut(s) 74, 146, 701
BccI CCATC 3 cut(s) 422, 555, 1157
BceAI ACGGC 3 cut(s) 440, 782, 1169
BciT130I CCWGG 6 cut(s) 202, 224, 296, 673, 707, 1114
BclI TGATCA 1 cut(s) 336
BcnI CCSGG 1 cut(s) 967
BfaI CTAG 1 cut(s) 1386
BfmI CTRYAG 1 cut(s) 625
BfoI RGCGCY 2 cut(s) 765, 1194
BisI GCNGC 4 cut(s) 63, 135, 690, 1278
BlsI GCNGC 4 cut(s) 64, 136, 691, 1279
Bme1390I CCNGG 7 cut(s) 202, 224, 296, 673, 707, 967, 1114
Bme18I GGWCC 6 cut(s) 324, 440, 567, 636, 731, 1169
BmeRI GACNNNNNGTC 1 cut(s) 797
BmeT110I CYCGRG 2 cut(s) 280, 1303
BmgT120I GGNCC 6 cut(s) 324, 440, 567, 636, 731, 1169
BmiI GGNNCC 4 cut(s) 187, 441, 763, 1171
BmrFI CCNGG 7 cut(s) 202, 224, 296, 673, 707, 967, 1114
BmrI ACTGGG 2 cut(s) 278, 922
BmsI GCATC 3 cut(s) 224, 973, 990
BmtI GCTAGC 1 cut(s) 1389
BmuI ACTGGG 2 cut(s) 278, 922
BpiI GAAGAC 2 cut(s) 292, 595
Bpu10I CCTNAGC 2 cut(s) 231, 1231
Bpu14I TTCGAA 1 cut(s) 417
BpuMI CCSGG 1 cut(s) 967
BsaAI YACGTR 1 cut(s) 1324
BsaHI GRCGYC 2 cut(s) 198, 762
BsaJI CCNNGG 1 cut(s) 965
BsaXI ACNNNNNCTCC 2 cut(s) 245, 275
Bsc4I CCNNNNNNNGG 3 cut(s) 645, 1166, 1276
Bse118I RCCGGY 1 cut(s) 758
Bse1I ACTGG 7 cut(s) 273, 409, 452, 747, 917, 1012, 1444
Bse3DI GCAATG 3 cut(s) 63, 684, 828
BseBI CCWGG 6 cut(s) 202, 224, 296, 673, 707, 1114
BseDI CCNNGG 1 cut(s) 965
BseGI GGATG 5 cut(s) 405, 433, 501, 606, 660
BseLI CCNNNNNNNGG 3 cut(s) 645, 1166, 1276
BseMI GCAATG 3 cut(s) 63, 684, 828
BseMII CTCAG 4 cut(s) 49, 245, 741, 848
BseNI ACTGG 7 cut(s) 273, 409, 452, 747, 917, 1012, 1444
BseRI GAGGAG 2 cut(s) 476, 923
BseSI GKGCMC 1 cut(s) 1451
BseXI GCAGC 3 cut(s) 74, 146, 701
BseYI CCCAGC 2 cut(s) 436, 1462
BsgI GTGCAG 1 cut(s) 460
Bsh1236I CGCG 1 cut(s) 981
BshFI GGCC 3 cut(s) 467, 705, 1148
BshNI GGYRCC 1 cut(s) 761
BsiHKCI CYCGRG 2 cut(s) 280, 1303
BsiSI CCGG 2 cut(s) 759, 967
BslFI GGGAC 2 cut(s) 1155, 1445
BslI CCNNNNNNNGG 3 cut(s) 645, 1166, 1276
BsmFI GGGAC 2 cut(s) 1155, 1445
BsmI GAATGC 6 cut(s) 279, 342, 952, 1080, 1279, 1334
BsnI GGCC 3 cut(s) 467, 705, 1148
BsoBI CYCGRG 2 cut(s) 280, 1303
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 1451
Bsp143I GATC 5 cut(s) 38, 139, 185, 336, 494
BspACI CCGC 7 cut(s) 183, 327, 789, 986, 1227, 1257, 1277
BspANI GGCC 3 cut(s) 467, 705, 1148
BspCNI CTCAG 4 cut(s) 48, 244, 740, 847
BspFNI CGCG 1 cut(s) 981
BspLI GGNNCC 4 cut(s) 187, 441, 763, 1171
BspOI GCTAGC 1 cut(s) 1389
BspPI GGATC 3 cut(s) 180, 193, 502
BspT104I TTCGAA 1 cut(s) 417
BspT107I GGYRCC 1 cut(s) 761
BsrDI GCAATG 3 cut(s) 63, 684, 828
BsrFI RCCGGY 1 cut(s) 758
BsrI ACTGG 7 cut(s) 273, 409, 452, 747, 917, 1012, 1444
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 1 cut(s) 965
BssMI GATC 5 cut(s) 38, 139, 185, 336, 494
BssNI GRCGYC 2 cut(s) 198, 762
Bst2UI CCWGG 6 cut(s) 202, 224, 296, 673, 707, 1114
Bst4CI ACNGT 4 cut(s) 106, 601, 805, 1213
BstACI GRCGYC 2 cut(s) 198, 762
BstAPI GCANNNNNTGC 1 cut(s) 590
BstBAI YACGTR 1 cut(s) 1324
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 4 cut(s) 523, 819, 1267, 1387
BstDEI CTNAG 6 cut(s) 35, 231, 727, 834, 972, 1231
BstF5I GGATG 5 cut(s) 405, 433, 501, 606, 660
BstFNI CGCG 1 cut(s) 981
BstH2I RGCGCY 2 cut(s) 765, 1194
BstHHI GCGC 3 cut(s) 764, 981, 1193
BstKTI GATC 5 cut(s) 41, 142, 188, 339, 497
BstMBI GATC 5 cut(s) 38, 139, 185, 336, 494
BstMWI GCNNNNNNNGC 6 cut(s) 62, 247, 590, 1190, 1314, 1446
BstNI CCWGG 6 cut(s) 202, 224, 296, 673, 707, 1114
BstNSI RCATGY 3 cut(s) 588, 821, 1321
BstSCI CCNGG 7 cut(s) 200, 222, 294, 671, 705, 965, 1112
BstSFI CTRYAG 1 cut(s) 625
BstSLI GKGCMC 1 cut(s) 1451
BstUI CGCG 1 cut(s) 981
BstV1I GCAGC 3 cut(s) 74, 146, 701
BstV2I GAAGAC 2 cut(s) 292, 595
BstX2I RGATCY 2 cut(s) 185, 494
BstXI CCANNNNNNTGG 1 cut(s) 512
BstYI RGATCY 2 cut(s) 185, 494
BsuRI GGCC 3 cut(s) 467, 705, 1148
BtgZI GCGATG 1 cut(s) 996
BtsCI GGATG 5 cut(s) 405, 433, 501, 606, 660
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 4 cut(s) 416, 666, 801, 1451
Cac8I GCNNGC 4 cut(s) 523, 819, 1267, 1387
CaiI CAGNNNCTG 1 cut(s) 238
CfoI GCGC 3 cut(s) 764, 981, 1193
Cfr10I RCCGGY 1 cut(s) 758
Cfr13I GGNCC 6 cut(s) 324, 440, 567, 636, 731, 1169
CseI GACGC 1 cut(s) 206
CsiI ACCWGGT 1 cut(s) 222
Csp6I GTAC 4 cut(s) 676, 910, 1038, 1321
CviQI GTAC 4 cut(s) 676, 910, 1038, 1321
DdeI CTNAG 6 cut(s) 35, 231, 727, 834, 972, 1231
DinI GGCGCC 1 cut(s) 763
DpnI GATC 5 cut(s) 40, 141, 187, 338, 496
DpnII GATC 5 cut(s) 38, 139, 185, 336, 494
DriI GACNNNNNGTC 1 cut(s) 797
EaeI YGGCCR 2 cut(s) 703, 1146
Eam1105I GACNNNNNGTC 1 cut(s) 797
EciI GGCGGA 3 cut(s) 198, 804, 1246
Eco47I GGWCC 6 cut(s) 324, 440, 567, 636, 731, 1169
Eco88I CYCGRG 2 cut(s) 280, 1303
EcoO109I RGGNCCY 1 cut(s) 440
EcoRII CCWGG 6 cut(s) 200, 222, 294, 671, 705, 1112
EcoT22I ATGCAT 2 cut(s) 819, 1080
EgeI GGCGCC 1 cut(s) 763
EheI GGCGCC 1 cut(s) 763
FaqI GGGAC 2 cut(s) 1155, 1445
FauI CCCGC 2 cut(s) 979, 1234
FauNDI CATATG 2 cut(s) 1094, 1239
FbaI TGATCA 1 cut(s) 336
FblI GTMKAC 2 cut(s) 192, 1215
Fnu4HI GCNGC 4 cut(s) 63, 135, 690, 1278
FokI GGATG 5 cut(s) 392, 440, 488, 593, 667
Fsp4HI GCNGC 4 cut(s) 63, 135, 690, 1278
FspBI CTAG 1 cut(s) 1386
GlaI GCGC 3 cut(s) 763, 980, 1192
GluI GCNGC 4 cut(s) 63, 135, 690, 1278
GsaI CCCAGC 2 cut(s) 440, 1466
HaeII RGCGCY 2 cut(s) 765, 1194
HaeIII GGCC 3 cut(s) 467, 705, 1148
HapII CCGG 2 cut(s) 759, 967
HgaI GACGC 1 cut(s) 206
HhaI GCGC 3 cut(s) 764, 981, 1193
Hin1I GRCGYC 2 cut(s) 198, 762
Hin6I GCGC 3 cut(s) 762, 979, 1191
HinP1I GCGC 3 cut(s) 762, 979, 1191
HincII GTYRAC 2 cut(s) 109, 1414
HindII GTYRAC 2 cut(s) 109, 1414
HindIII AAGCTT 1 cut(s) 1286
HinfI GANTC 2 cut(s) 285, 309
HpaII CCGG 2 cut(s) 759, 967
HphI GGTGA 6 cut(s) 748, 1001, 1138, 1201, 1237, 1339
Hpy166II GTNNAC 7 cut(s) 109, 193, 879, 910, 1216, 1330, 1414
Hpy188I TCNGA 6 cut(s) 38, 79, 164, 336, 837, 1376
Hpy188III TCNNGA 5 cut(s) 282, 394, 551, 729, 1303
Hpy8I GTNNAC 7 cut(s) 109, 193, 879, 910, 1216, 1330, 1414
Hpy99I CGWCG 2 cut(s) 200, 356
HpyCH4III ACNGT 4 cut(s) 106, 601, 805, 1213
HpyCH4IV ACGT 3 cut(s) 159, 351, 1323
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 247, 590, 1190, 1314, 1446
HpyF3I CTNAG 6 cut(s) 35, 231, 727, 834, 972, 1231
HpySE526I ACGT 3 cut(s) 159, 351, 1323
Hsp92I GRCGYC 2 cut(s) 198, 762
HspAI GCGC 3 cut(s) 762, 979, 1191
KasI GGCGCC 1 cut(s) 761
Ksp22I TGATCA 1 cut(s) 336
Kzo9I GATC 5 cut(s) 38, 139, 185, 336, 494
LmnI GCTCC 3 cut(s) 318, 936, 963
Lsp1109I GCAGC 3 cut(s) 74, 146, 701
LweI GCATC 3 cut(s) 224, 973, 990
MabI ACCWGGT 1 cut(s) 222
MaeI CTAG 1 cut(s) 1386
MaeII ACGT 3 cut(s) 159, 351, 1323
MaeIII GTNAC 3 cut(s) 799, 991, 1390
MalI GATC 5 cut(s) 40, 141, 187, 338, 496
MboI GATC 5 cut(s) 38, 139, 185, 336, 494
MboII GAAGA 7 cut(s) 131, 134, 137, 292, 595, 1156, 1288
MfeI CAATTG 1 cut(s) 1107
MflI RGATCY 2 cut(s) 185, 494
MhlI GDGCHC 1 cut(s) 1451
MlsI TGGCCA 2 cut(s) 705, 1148
MluCI AATT 3 cut(s) 1068, 1107, 1177
MluNI TGGCCA 2 cut(s) 705, 1148
Mly113I GGCGCC 1 cut(s) 762
MmeI TCCRAC 2 cut(s) 405, 439
Mox20I TGGCCA 2 cut(s) 705, 1148
Mph1103I ATGCAT 2 cut(s) 819, 1080
MroXI GAANNNNTTC 2 cut(s) 950, 1334
MscI TGGCCA 2 cut(s) 705, 1148
MseI TTAA 4 cut(s) 45, 156, 843, 1176
MslI CAYNNNNRTG 4 cut(s) 945, 998, 1322, 1343
Msp20I TGGCCA 2 cut(s) 705, 1148
MspA1I CMGCKG 1 cut(s) 210
MspI CCGG 2 cut(s) 759, 967
MspR9I CCNGG 7 cut(s) 202, 224, 296, 673, 707, 967, 1114
MunI CAATTG 1 cut(s) 1107
Mva1269I GAATGC 6 cut(s) 279, 342, 952, 1080, 1279, 1334
MvaI CCWGG 6 cut(s) 202, 224, 296, 673, 707, 1114
MvnI CGCG 1 cut(s) 981
MwoI GCNNNNNNNGC 6 cut(s) 62, 247, 590, 1190, 1314, 1446
NarI GGCGCC 1 cut(s) 762
NciI CCSGG 1 cut(s) 967
NdeI CATATG 2 cut(s) 1094, 1239
NdeII GATC 5 cut(s) 38, 139, 185, 336, 494
NheI GCTAGC 1 cut(s) 1385
NlaIV GGNNCC 4 cut(s) 187, 441, 763, 1171
NmuCI GTSAC 3 cut(s) 799, 991, 1390
NsiI ATGCAT 2 cut(s) 819, 1080
NspI RCATGY 3 cut(s) 588, 821, 1321
NspV TTCGAA 1 cut(s) 417
PaeI GCATGC 1 cut(s) 821
PcsI WCGNNNNNNNCGW 1 cut(s) 1338
PctI GAATGC 6 cut(s) 279, 342, 952, 1080, 1279, 1334
PdmI GAANNNNTTC 2 cut(s) 950, 1334
PfeI GAWTC 2 cut(s) 285, 309
PkrI GCNGC 4 cut(s) 64, 136, 691, 1279
PluTI GGCGCC 1 cut(s) 765
Ppu21I YACGTR 1 cut(s) 1324
PpuMI RGGWCCY 1 cut(s) 440
PshBI ATTAAT 1 cut(s) 1176
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 6 cut(s) 200, 222, 294, 671, 705, 1112
PspFI CCCAGC 2 cut(s) 436, 1462
PspGI CCWGG 6 cut(s) 200, 222, 294, 671, 705, 1112
PspN4I GGNNCC 4 cut(s) 187, 441, 763, 1171
PspPI GGNCC 6 cut(s) 324, 440, 567, 636, 731, 1169
PspPPI RGGWCCY 1 cut(s) 440
PstNI CAGNNNCTG 1 cut(s) 238
PsuI RGATCY 2 cut(s) 185, 494
PvuII CAGCTG 1 cut(s) 210
RsaI GTAC 4 cut(s) 677, 911, 1039, 1322
RsaNI GTAC 4 cut(s) 676, 910, 1038, 1321
RseI CAYNNNNRTG 4 cut(s) 945, 998, 1322, 1343
SaqAI TTAA 4 cut(s) 45, 156, 843, 1176
SatI GCNGC 4 cut(s) 63, 135, 690, 1278
Sau3AI GATC 5 cut(s) 38, 139, 185, 336, 494
Sau96I GGNCC 6 cut(s) 324, 440, 567, 636, 731, 1169
ScrFI CCNGG 7 cut(s) 202, 224, 296, 673, 707, 967, 1114
SduI GDGCHC 1 cut(s) 1451
SexAI ACCWGGT 1 cut(s) 222
SfaNI GCATC 3 cut(s) 224, 973, 990
SfcI CTRYAG 1 cut(s) 625
SfoI GGCGCC 1 cut(s) 763
SfuI TTCGAA 1 cut(s) 417
SgrAI CRCCGGYG 1 cut(s) 758
SinI GGWCC 6 cut(s) 324, 440, 567, 636, 731, 1169
SmiMI CAYNNNNRTG 4 cut(s) 945, 998, 1322, 1343
SphI GCATGC 1 cut(s) 821
Sse9I AATT 3 cut(s) 1068, 1107, 1177
SsiI CCGC 7 cut(s) 183, 327, 789, 986, 1227, 1257, 1277
SspDI GGCGCC 1 cut(s) 761
SspMI CTAG 1 cut(s) 1386
StyD4I CCNGG 7 cut(s) 200, 222, 294, 671, 705, 965, 1112
TaaI ACNGT 4 cut(s) 106, 601, 805, 1213
TaiI ACGT 3 cut(s) 162, 354, 1326
TaqI TCGA 5 cut(s) 218, 354, 361, 417, 944
TaqII GACCGA 1 cut(s) 1157
TasI AATT 3 cut(s) 1068, 1107, 1177
TatI WGTACW 1 cut(s) 909
TauI GCSGC 1 cut(s) 1280
TfiI GAWTC 2 cut(s) 285, 309
Tru1I TTAA 4 cut(s) 45, 156, 843, 1176
Tru9I TTAA 4 cut(s) 45, 156, 843, 1176
TscAI CASTG 4 cut(s) 416, 673, 808, 1451
TseFI GTSAC 3 cut(s) 799, 991, 1390
TseI GCWGC 3 cut(s) 62, 134, 689
Tsp45I GTSAC 3 cut(s) 799, 991, 1390
TspDTI ATGAA 5 cut(s) 595, 689, 1006, 1327, 1390
TspGWI ACGGA 5 cut(s) 39, 178, 321, 882, 1281
TspRI CASTG 4 cut(s) 416, 673, 808, 1451
VpaK11BI GGWCC 6 cut(s) 324, 440, 567, 636, 731, 1169
VspI ATTAAT 1 cut(s) 1176
XceI RCATGY 3 cut(s) 588, 821, 1321
XcmI CCANNNNNNNNNTGG 1 cut(s) 1120
XmiI GTMKAC 2 cut(s) 192, 1215
XmnI GAANNNNTTC 2 cut(s) 950, 1334
XspI CTAG 1 cut(s) 1386
Zsp2I ATGCAT 2 cut(s) 819, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.