MD01G1149900.v1.1

Pleckstrin homology domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006020
Physical Location & Seq
Reverse (-)
25871577 .. 25872008
432 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1149900.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGCGAGCCTATGGCGGGCCGCGATGGGCCAGACGGTGCAACCCAACGACTACGACGGCATCGAGTTCTGGTCAAACCCCGAACGCACCGGTTGGTTGACTAAACAAGGCGAGTACATAAAGACGTGGCGTCGCCGGTGGTTCGTCCTCAAGCAAGGCAAGCTTTTCTGGTTCATGGACTCCGCCGTCAACCGCGGCTCCAGGCCACGTGGCGTGATCCCGGTGGCGTCGTGCCTCACAGTGAAGGGAGCCGAGGACGTCCTCAACAAGCAGTACGCGTTCGAGCTGTCGACGCGCTCCGAGACGATGTACTTCATCGCCGACTCGGAGAAGGAGAAGGAGGACTGGATCAACTCGATCGGACGGTCCATAGTGCAGCACTCGAGGTCCGTCACCGACTCCGAGGTCGTCGATTATGATAGCAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0032266 GO:0035091 GO:0043167 GO:0043168 GO:1901981
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

144

Amino Acids

16.62

Weight (kDa)

8.68

Isoelectric Point (pI)

63.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 28 - 124 5.9e-20 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 185, 404
AatII GACGTC 1 cut(s) 261
AccI GTMKAC 1 cut(s) 290
AccII CGCG 4 cut(s) 23, 195, 278, 295
AciI CCGC 5 cut(s) 16, 21, 183, 193, 195
AclWI GGATC 2 cut(s) 211, 356
AcvI CACGTG 1 cut(s) 209
AcyI GRCGYC 3 cut(s) 130, 227, 258
AfaI GTAC 3 cut(s) 116, 275, 311
AfiI CCNNNNNNNGG 1 cut(s) 16
AflIII ACRYGT 1 cut(s) 276
AgeI ACCGGT 1 cut(s) 89
AhdI GACNNNNNGTC 1 cut(s) 129
AjiI CACGTC 1 cut(s) 126
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 2 cut(s) 163, 286
AluI AGCT 2 cut(s) 163, 286
Alw26I GTCTC 1 cut(s) 296
AlwI GGATC 2 cut(s) 211, 356
Ama87I CYCGRG 1 cut(s) 382
AoxI GGCC 3 cut(s) 18, 28, 203
ApeKI GCWGC 1 cut(s) 376
AsiGI ACCGGT 1 cut(s) 89
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 4 cut(s) 18, 28, 366, 387
AsuC2I CCSGG 1 cut(s) 221
AsuHPI GGTGA 1 cut(s) 385
AvaI CYCGRG 1 cut(s) 382
AvaII GGWCC 2 cut(s) 366, 387
BbrPI CACGTG 1 cut(s) 209
BbvI GCAGC 1 cut(s) 388
BccI CCATC 1 cut(s) 19
BceAI ACGGC 2 cut(s) 73, 170
BciT130I CCWGG 1 cut(s) 202
BcnI CCSGG 1 cut(s) 221
BcoDI GTCTC 1 cut(s) 296
BisI GCNGC 3 cut(s) 21, 196, 377
BlsI GCNGC 3 cut(s) 22, 197, 378
Bme1390I CCNGG 2 cut(s) 202, 221
Bme18I GGWCC 2 cut(s) 366, 387
BmeRI GACNNNNNGTC 1 cut(s) 129
BmeT110I CYCGRG 1 cut(s) 382
BmgBI CACGTC 1 cut(s) 126
BmgT120I GGNCC 4 cut(s) 18, 28, 366, 387
BmiI GGNNCC 2 cut(s) 199, 250
BmrFI CCNGG 2 cut(s) 202, 221
BmsI GCATC 1 cut(s) 69
BpmI CTGGAG 1 cut(s) 184
BpuEI CTTGAG 1 cut(s) 134
BpuMI CCSGG 1 cut(s) 221
BsaAI YACGTR 1 cut(s) 209
BsaHI GRCGYC 3 cut(s) 130, 227, 258
BsaJI CCNNGG 3 cut(s) 193, 252, 402
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 1 cut(s) 16
Bse118I RCCGGY 2 cut(s) 89, 135
Bse1I ACTGG 1 cut(s) 350
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 3 cut(s) 193, 252, 402
BseLI CCNNNNNNNGG 1 cut(s) 16
BseNI ACTGG 1 cut(s) 350
BseXI GCAGC 1 cut(s) 388
BsgI GTGCAG 1 cut(s) 395
Bsh1236I CGCG 4 cut(s) 23, 195, 278, 295
Bsh1285I CGRYCG 1 cut(s) 360
BshFI GGCC 3 cut(s) 20, 30, 205
BshTI ACCGGT 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 360
BsiHKCI CYCGRG 1 cut(s) 382
BsiSI CCGG 3 cut(s) 90, 136, 221
BslI CCNNNNNNNGG 1 cut(s) 16
BsmAI GTCTC 1 cut(s) 296
BsmBI CGTCTC 1 cut(s) 296
BsnI GGCC 3 cut(s) 20, 30, 205
BsoBI CYCGRG 1 cut(s) 382
Bsp143I GATC 3 cut(s) 216, 348, 357
BspACI CCGC 5 cut(s) 16, 21, 183, 193, 195
BspANI GGCC 3 cut(s) 20, 30, 205
BspFNI CGCG 4 cut(s) 23, 195, 278, 295
BspLI GGNNCC 2 cut(s) 199, 250
BspPI GGATC 2 cut(s) 211, 356
BsrFI RCCGGY 2 cut(s) 89, 135
BsrI ACTGG 1 cut(s) 350
BssAI RCCGGY 2 cut(s) 89, 135
BssECI CCNNGG 3 cut(s) 193, 252, 402
BssMI GATC 3 cut(s) 216, 348, 357
BssNI GRCGYC 3 cut(s) 130, 227, 258
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 3 cut(s) 37, 241, 366
BstACI GRCGYC 3 cut(s) 130, 227, 258
BstBAI YACGTR 1 cut(s) 209
BstC8I GCNNGC 3 cut(s) 7, 18, 161
BstDSI CCRYGG 1 cut(s) 193
BstFNI CGCG 4 cut(s) 23, 195, 278, 295
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 3 cut(s) 219, 351, 360
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 3 cut(s) 216, 348, 357
BstMCI CGRYCG 1 cut(s) 360
BstMWI GCNNNNNNNGC 2 cut(s) 160, 292
BstNI CCWGG 1 cut(s) 202
BstSCI CCNGG 2 cut(s) 200, 219
BstUI CGCG 4 cut(s) 23, 195, 278, 295
BstV1I GCAGC 1 cut(s) 388
BsuRI GGCC 3 cut(s) 20, 30, 205
BtgI CCRYGG 1 cut(s) 193
BtgZI GCGATG 2 cut(s) 38, 301
BtrI CACGTC 1 cut(s) 126
BtsIMutI CAGTG 1 cut(s) 246
Cac8I GCNNGC 3 cut(s) 7, 18, 161
CfoI GCGC 1 cut(s) 297
Cfr10I RCCGGY 2 cut(s) 89, 135
Cfr13I GGNCC 4 cut(s) 18, 28, 366, 387
Cfr42I CCGCGG 1 cut(s) 196
CseI GACGC 3 cut(s) 119, 216, 301
Csp6I GTAC 3 cut(s) 115, 274, 310
CspAI ACCGGT 1 cut(s) 89
CviAII CATG 1 cut(s) 175
CviJI RGCY 8 cut(s) 9, 20, 30, 163, 198, 205, 251, 286
CviKI_1 RGCY 8 cut(s) 9, 20, 30, 163, 198, 205, 251, 286
CviQI GTAC 3 cut(s) 115, 274, 310
DpnI GATC 3 cut(s) 218, 350, 359
DpnII GATC 3 cut(s) 216, 348, 357
DrdI GACNNNNNNGTC 2 cut(s) 185, 404
DriI GACNNNNNGTC 1 cut(s) 129
DseDI GACNNNNNNGTC 2 cut(s) 185, 404
Eam1105I GACNNNNNGTC 1 cut(s) 129
EciI GGCGGA 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 366, 387
Eco72I CACGTG 1 cut(s) 209
Eco88I CYCGRG 1 cut(s) 382
EcoRII CCWGG 1 cut(s) 200
Esp3I CGTCTC 1 cut(s) 296
FaeI CATG 1 cut(s) 178
FaiI YATR 5 cut(s) 13, 119, 176, 371, 417
FalI AAGNNNNNCTT 2 cut(s) 147, 179
FatI CATG 1 cut(s) 174
FauI CCCGC 1 cut(s) 9
FblI GTMKAC 1 cut(s) 290
Fnu4HI GCNGC 3 cut(s) 21, 196, 377
Fsp4HI GCNGC 3 cut(s) 21, 196, 377
GlaI GCGC 1 cut(s) 296
GluI GCNGC 3 cut(s) 21, 196, 377
GsuI CTGGAG 1 cut(s) 184
HaeIII GGCC 3 cut(s) 20, 30, 205
HapII CCGG 3 cut(s) 90, 136, 221
HgaI GACGC 3 cut(s) 119, 216, 301
HhaI GCGC 1 cut(s) 297
Hin1I GRCGYC 3 cut(s) 130, 227, 258
Hin1II CATG 1 cut(s) 178
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HincII GTYRAC 3 cut(s) 99, 190, 291
HindII GTYRAC 3 cut(s) 99, 190, 291
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 3 cut(s) 179, 323, 398
HpaII CCGG 3 cut(s) 90, 136, 221
HphI GGTGA 1 cut(s) 385
Hpy166II GTNNAC 3 cut(s) 99, 190, 291
Hpy188I TCNGA 4 cut(s) 301, 328, 362, 403
Hpy8I GTNNAC 3 cut(s) 99, 190, 291
Hpy99I CGWCG 5 cut(s) 59, 135, 232, 295, 413
HpyAV CCTTC 3 cut(s) 238, 325, 331
HpyCH4III ACNGT 3 cut(s) 37, 241, 366
HpyCH4IV ACGT 3 cut(s) 125, 208, 258
HpyCH4V TGCA 2 cut(s) 40, 376
HpyF10VI GCNNNNNNNGC 2 cut(s) 160, 292
HpySE526I ACGT 3 cut(s) 125, 208, 258
Hsp92I GRCGYC 3 cut(s) 130, 227, 258
Hsp92II CATG 1 cut(s) 178
HspAI GCGC 1 cut(s) 295
KspI CCGCGG 1 cut(s) 196
Kzo9I GATC 3 cut(s) 216, 348, 357
LmnI GCTCC 3 cut(s) 203, 248, 302
LpnPI CCDG 9 cut(s) 44, 55, 103, 149, 154, 187, 214, 234, 331
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 1 cut(s) 69
MaeII ACGT 3 cut(s) 125, 208, 258
MaeIII GTNAC 1 cut(s) 391
MalI GATC 3 cut(s) 218, 350, 359
MboI GATC 3 cut(s) 216, 348, 357
MluI ACGCGT 1 cut(s) 276
MlyI GAGTC 3 cut(s) 173, 317, 392
MnlI CCTC 7 cut(s) 158, 245, 247, 272, 334, 378, 397
MspA1I CMGCKG 1 cut(s) 195
MspI CCGG 3 cut(s) 90, 136, 221
MspR9I CCNGG 2 cut(s) 202, 221
MvaI CCWGG 1 cut(s) 202
MvnI CGCG 4 cut(s) 23, 195, 278, 295
MwoI GCNNNNNNNGC 2 cut(s) 160, 292
NciI CCSGG 1 cut(s) 221
NdeII GATC 3 cut(s) 216, 348, 357
NlaIII CATG 1 cut(s) 178
NlaIV GGNNCC 2 cut(s) 199, 250
NmeAIII GCCGAG 1 cut(s) 277
NmuCI GTSAC 1 cut(s) 391
PaeR7I CTCGAG 1 cut(s) 382
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PinAI ACCGGT 1 cut(s) 89
PkrI GCNGC 3 cut(s) 22, 197, 378
Ple19I CGATCG 1 cut(s) 360
PleI GAGTC 3 cut(s) 173, 317, 392
PmaCI CACGTG 1 cut(s) 209
PmlI CACGTG 1 cut(s) 209
PpsI GAGTC 3 cut(s) 173, 317, 392
Ppu21I YACGTR 1 cut(s) 209
Psp6I CCWGG 1 cut(s) 200
PspCI CACGTG 1 cut(s) 209
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 2 cut(s) 199, 250
PspPI GGNCC 4 cut(s) 18, 28, 366, 387
PspXI VCTCGAGB 1 cut(s) 382
PvuI CGATCG 1 cut(s) 360
RsaI GTAC 3 cut(s) 116, 275, 311
RsaNI GTAC 3 cut(s) 115, 274, 310
SacII CCGCGG 1 cut(s) 196
SalI GTCGAC 1 cut(s) 289
SatI GCNGC 3 cut(s) 21, 196, 377
Sau3AI GATC 3 cut(s) 216, 348, 357
Sau96I GGNCC 4 cut(s) 18, 28, 366, 387
SchI GAGTC 3 cut(s) 173, 317, 392
ScrFI CCNGG 2 cut(s) 202, 221
SetI ASST 7 cut(s) 128, 165, 211, 261, 288, 389, 408
SfaNI GCATC 1 cut(s) 69
Sfr274I CTCGAG 1 cut(s) 382
Sfr303I CCGCGG 1 cut(s) 196
SgrAI CRCCGGYG 1 cut(s) 135
SgrBI CCGCGG 1 cut(s) 196
SinI GGWCC 2 cut(s) 366, 387
SlaI CTCGAG 1 cut(s) 382
SmlI CTYRAG 2 cut(s) 149, 382
SmoI CTYRAG 2 cut(s) 149, 382
SsiI CCGC 5 cut(s) 16, 21, 183, 193, 195
StyD4I CCNGG 2 cut(s) 200, 219
TaaI ACNGT 3 cut(s) 37, 241, 366
TaiI ACGT 3 cut(s) 128, 211, 261
TaqI TCGA 6 cut(s) 63, 282, 290, 356, 383, 411
TatI WGTACW 2 cut(s) 114, 309
TauI GCSGC 2 cut(s) 23, 198
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 1 cut(s) 391
TseI GCWGC 1 cut(s) 376
Tsp45I GTSAC 1 cut(s) 391
TspDTI ATGAA 2 cut(s) 163, 304
TspGWI ACGGA 1 cut(s) 379
TspRI CASTG 1 cut(s) 246
VpaK11BI GGWCC 2 cut(s) 366, 387
XhoI CTCGAG 1 cut(s) 382
XmiI GTMKAC 1 cut(s) 290
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.