RLG00000027085

Pleckstrin homology domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0006020
Physical Location & Seq
Forward (+)
8290692 .. 8291110
419 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027085

Sequence Viewer

Length: 375 bp
ATGGCGAGTCTGTGGCCGGCCGCGACGGGCCTGACCGATAAGGCCGACGACCACGACGGCGTGGAGTTCTGGTCAAACCCGGAGTGCACGGGGTGGTTGACCAAGCAAGGGAAGGACTCCGCCGTCACGCGCGAGTCAAGGACACGCGGCGTGATCCCGGTGGCCACGTGCCTGACGGTGAAGGGCGCGGAGGACGTGCTGAACAAGCAGTACGCGTTCGAGCTCTCCACGCGCTCCTGGACCATGTACTTCATCGCCGACTCCGAGAACGAGAAGGAGGACTGGATCAACTCGATCGGACGGTCCACCGTGCAGCACTCCAGGTCCGTCACCGACTCCGAGGTCGTCGATTACGATAGCAACAACAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005543 GO:0008289 GO:0032266 GO:0035091 GO:0043167 GO:0043168 GO:1901981
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

125

Amino Acids

13.87

Weight (kDa)

4.72

Isoelectric Point (pI)

56.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 44 - 101 6.6e-06 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 122, 341
AccII CGCG 7 cut(s) 23, 130, 132, 147, 188, 215, 232
AciI CCGC 4 cut(s) 21, 120, 147, 188
AclWI GGATC 2 cut(s) 148, 293
AcoI YGGCCR 3 cut(s) 14, 18, 162
AcvI CACGTG 1 cut(s) 168
AdeI CACNNNGTG 1 cut(s) 93
AfaI GTAC 2 cut(s) 212, 248
AfiI CCNNNNNNNGG 1 cut(s) 108
AflIII ACRYGT 1 cut(s) 213
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 2 cut(s) 236, 320
AluBI AGCT 1 cut(s) 223
AluI AGCT 1 cut(s) 223
Alw21I GWGCWC 2 cut(s) 89, 225
Alw44I GTGCAC 1 cut(s) 85
AlwI GGATC 2 cut(s) 148, 293
AoxI GGCC 5 cut(s) 14, 18, 28, 42, 162
ApaLI GTGCAC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 313
AspLEI GCGC 3 cut(s) 132, 188, 234
AspS9I GGNCC 4 cut(s) 28, 240, 303, 324
AsuC2I CCSGG 2 cut(s) 80, 158
AsuHPI GGTGA 2 cut(s) 190, 322
AvaII GGWCC 3 cut(s) 240, 303, 324
BaeGI GKGCMC 1 cut(s) 89
BalI TGGCCA 1 cut(s) 164
BanII GRGCYC 1 cut(s) 225
BbrPI CACGTG 1 cut(s) 168
Bbv12I GWGCWC 2 cut(s) 89, 225
BbvI GCAGC 1 cut(s) 325
BceAI ACGGC 2 cut(s) 73, 107
BciT130I CCWGG 2 cut(s) 238, 322
BcnI CCSGG 2 cut(s) 80, 158
BisI GCNGC 3 cut(s) 21, 148, 314
BlsI GCNGC 3 cut(s) 22, 149, 315
Bme1390I CCNGG 4 cut(s) 80, 158, 238, 322
Bme18I GGWCC 3 cut(s) 240, 303, 324
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 4 cut(s) 28, 240, 303, 324
BmrFI CCNGG 4 cut(s) 80, 158, 238, 322
BpmI CTGGAG 1 cut(s) 304
BpuMI CCSGG 2 cut(s) 80, 158
BsaAI YACGTR 1 cut(s) 168
BsaJI CCNNGG 1 cut(s) 339
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 1 cut(s) 108
Bse118I RCCGGY 1 cut(s) 16
Bse1I ACTGG 1 cut(s) 287
BseBI CCWGG 2 cut(s) 238, 322
BseDI CCNNGG 1 cut(s) 339
BseLI CCNNNNNNNGG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 287
BseSI GKGCMC 1 cut(s) 89
BseX3I CGGCCG 1 cut(s) 18
BseXI GCAGC 1 cut(s) 325
BsgI GTGCAG 1 cut(s) 332
Bsh1236I CGCG 7 cut(s) 23, 130, 132, 147, 188, 215, 232
Bsh1285I CGRYCG 2 cut(s) 21, 297
BshFI GGCC 5 cut(s) 16, 20, 30, 44, 164
BsiEI CGRYCG 2 cut(s) 21, 297
BsiHKAI GWGCWC 2 cut(s) 89, 225
BsiSI CCGG 3 cut(s) 17, 80, 158
BslI CCNNNNNNNGG 1 cut(s) 108
BsnI GGCC 5 cut(s) 16, 20, 30, 44, 164
Bsp1286I GDGCHC 2 cut(s) 89, 225
Bsp143I GATC 3 cut(s) 153, 285, 294
BspACI CCGC 4 cut(s) 21, 120, 147, 188
BspANI GGCC 5 cut(s) 16, 20, 30, 44, 164
BspFNI CGCG 7 cut(s) 23, 130, 132, 147, 188, 215, 232
BspPI GGATC 2 cut(s) 148, 293
BsrFI RCCGGY 1 cut(s) 16
BsrI ACTGG 1 cut(s) 287
BssAI RCCGGY 1 cut(s) 16
BssECI CCNNGG 1 cut(s) 339
BssMI GATC 3 cut(s) 153, 285, 294
Bst2UI CCWGG 2 cut(s) 238, 322
Bst4CI ACNGT 3 cut(s) 178, 303, 310
BstBAI YACGTR 1 cut(s) 168
BstC8I GCNNGC 1 cut(s) 18
BstFNI CGCG 7 cut(s) 23, 130, 132, 147, 188, 215, 232
BstHHI GCGC 3 cut(s) 132, 188, 234
BstKTI GATC 3 cut(s) 156, 288, 297
BstMBI GATC 3 cut(s) 153, 285, 294
BstMCI CGRYCG 2 cut(s) 21, 297
BstMWI GCNNNNNNNGC 2 cut(s) 205, 229
BstNI CCWGG 2 cut(s) 238, 322
BstSCI CCNGG 4 cut(s) 78, 156, 236, 320
BstSLI GKGCMC 1 cut(s) 89
BstUI CGCG 7 cut(s) 23, 130, 132, 147, 188, 215, 232
BstV1I GCAGC 1 cut(s) 325
BstZI CGGCCG 1 cut(s) 18
BsuRI GGCC 5 cut(s) 16, 20, 30, 44, 164
BtgZI GCGATG 1 cut(s) 238
BtrI CACGTC 1 cut(s) 196
Cac8I GCNNGC 1 cut(s) 18
CfoI GCGC 3 cut(s) 132, 188, 234
Cfr10I RCCGGY 1 cut(s) 16
Cfr13I GGNCC 4 cut(s) 28, 240, 303, 324
Csp6I GTAC 2 cut(s) 211, 247
CviAII CATG 1 cut(s) 244
CviJI RGCY 6 cut(s) 16, 20, 30, 44, 164, 223
CviKI_1 RGCY 6 cut(s) 16, 20, 30, 44, 164, 223
CviQI GTAC 2 cut(s) 211, 247
DpnI GATC 3 cut(s) 155, 287, 296
DpnII GATC 3 cut(s) 153, 285, 294
DraIII CACNNNGTG 1 cut(s) 93
DrdI GACNNNNNNGTC 2 cut(s) 122, 341
DseDI GACNNNNNNGTC 2 cut(s) 122, 341
EaeI YGGCCR 3 cut(s) 14, 18, 162
EagI CGGCCG 1 cut(s) 18
EciI GGCGGA 1 cut(s) 109
Ecl136II GAGCTC 1 cut(s) 223
EclXI CGGCCG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 3 cut(s) 240, 303, 324
Eco52I CGGCCG 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 223
Eco72I CACGTG 1 cut(s) 168
EcoICRI GAGCTC 1 cut(s) 223
EcoRII CCWGG 2 cut(s) 236, 320
EcoT38I GRGCYC 1 cut(s) 225
FaeI CATG 1 cut(s) 247
FaiI YATR 1 cut(s) 245
FatI CATG 1 cut(s) 243
Fnu4HI GCNGC 3 cut(s) 21, 148, 314
FriOI GRGCYC 1 cut(s) 225
FseI GGCCGGCC 1 cut(s) 20
Fsp4HI GCNGC 3 cut(s) 21, 148, 314
GlaI GCGC 3 cut(s) 131, 187, 233
GluI GCNGC 3 cut(s) 21, 148, 314
GsuI CTGGAG 1 cut(s) 304
HaeIII GGCC 5 cut(s) 16, 20, 30, 44, 164
HapII CCGG 3 cut(s) 17, 80, 158
HhaI GCGC 3 cut(s) 132, 188, 234
Hin1II CATG 1 cut(s) 247
Hin6I GCGC 3 cut(s) 130, 186, 232
HinP1I GCGC 3 cut(s) 130, 186, 232
HincII GTYRAC 1 cut(s) 99
HindII GTYRAC 1 cut(s) 99
HinfI GANTC 5 cut(s) 7, 116, 134, 260, 335
HpaII CCGG 3 cut(s) 17, 80, 158
HphI GGTGA 2 cut(s) 190, 322
Hpy166II GTNNAC 3 cut(s) 87, 99, 306
Hpy188I TCNGA 3 cut(s) 265, 299, 340
Hpy8I GTNNAC 3 cut(s) 87, 99, 306
Hpy99I CGWCG 4 cut(s) 28, 50, 59, 350
HpyAV CCTTC 3 cut(s) 106, 175, 268
HpyCH4III ACNGT 3 cut(s) 178, 303, 310
HpyCH4IV ACGT 2 cut(s) 167, 195
HpyCH4V TGCA 2 cut(s) 87, 313
HpyF10VI GCNNNNNNNGC 2 cut(s) 205, 229
HpySE526I ACGT 2 cut(s) 167, 195
Hsp92II CATG 1 cut(s) 247
HspAI GCGC 3 cut(s) 130, 186, 232
KroI GCCGGC 1 cut(s) 16
KroNI GCCGGC 1 cut(s) 18
Kzo9I GATC 3 cut(s) 153, 285, 294
LmnI GCTCC 1 cut(s) 239
Lsp1109I GCAGC 1 cut(s) 325
MaeII ACGT 2 cut(s) 167, 195
MaeIII GTNAC 2 cut(s) 124, 328
MalI GATC 3 cut(s) 155, 287, 296
MboI GATC 3 cut(s) 153, 285, 294
MhlI GDGCHC 2 cut(s) 89, 225
MlsI TGGCCA 1 cut(s) 164
MluI ACGCGT 1 cut(s) 213
MluNI TGGCCA 1 cut(s) 164
MlyI GAGTC 5 cut(s) 16, 110, 143, 254, 329
MnlI CCTC 3 cut(s) 184, 271, 334
Mox20I TGGCCA 1 cut(s) 164
MroNI GCCGGC 1 cut(s) 16
MscI TGGCCA 1 cut(s) 164
Msp20I TGGCCA 1 cut(s) 164
MspI CCGG 3 cut(s) 17, 80, 158
MspR9I CCNGG 4 cut(s) 80, 158, 238, 322
MvaI CCWGG 2 cut(s) 238, 322
MvnI CGCG 7 cut(s) 23, 130, 132, 147, 188, 215, 232
MwoI GCNNNNNNNGC 2 cut(s) 205, 229
NaeI GCCGGC 1 cut(s) 18
NciI CCSGG 2 cut(s) 80, 158
NdeII GATC 3 cut(s) 153, 285, 294
NgoMIV GCCGGC 1 cut(s) 16
NlaIII CATG 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 124, 328
PcsI WCGNNNNNNNCGW 2 cut(s) 261, 351
PdiI GCCGGC 1 cut(s) 18
PfoI TCCNGGA 1 cut(s) 236
PkrI GCNGC 3 cut(s) 22, 149, 315
Ple19I CGATCG 1 cut(s) 297
PleI GAGTC 5 cut(s) 15, 110, 142, 254, 329
PmaCI CACGTG 1 cut(s) 168
PmlI CACGTG 1 cut(s) 168
PpsI GAGTC 5 cut(s) 15, 110, 142, 254, 329
Ppu21I YACGTR 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 2 cut(s) 236, 320
PspCI CACGTG 1 cut(s) 168
PspGI CCWGG 2 cut(s) 236, 320
PspPI GGNCC 4 cut(s) 28, 240, 303, 324
PsrI GAACNNNNNNTAC 2 cut(s) 194, 226
PvuI CGATCG 1 cut(s) 297
RigI GGCCGGCC 1 cut(s) 20
RsaI GTAC 2 cut(s) 212, 248
RsaNI GTAC 2 cut(s) 211, 247
SacI GAGCTC 1 cut(s) 225
SatI GCNGC 3 cut(s) 21, 148, 314
Sau3AI GATC 3 cut(s) 153, 285, 294
Sau96I GGNCC 4 cut(s) 28, 240, 303, 324
SchI GAGTC 5 cut(s) 16, 110, 143, 254, 329
ScrFI CCNGG 4 cut(s) 80, 158, 238, 322
SduI GDGCHC 2 cut(s) 89, 225
SetI ASST 5 cut(s) 170, 198, 225, 326, 345
SinI GGWCC 3 cut(s) 240, 303, 324
SsiI CCGC 4 cut(s) 21, 120, 147, 188
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 4 cut(s) 78, 156, 236, 320
TaaI ACNGT 3 cut(s) 178, 303, 310
TaiI ACGT 2 cut(s) 170, 198
TaqI TCGA 3 cut(s) 219, 293, 348
TaqII GACCGA 1 cut(s) 50
TatI WGTACW 1 cut(s) 246
TauI GCSGC 2 cut(s) 23, 150
TseFI GTSAC 2 cut(s) 124, 328
TseI GCWGC 1 cut(s) 313
Tsp45I GTSAC 2 cut(s) 124, 328
TspDTI ATGAA 1 cut(s) 241
TspGWI ACGGA 1 cut(s) 316
VneI GTGCAC 1 cut(s) 85
VpaK11BI GGWCC 3 cut(s) 240, 303, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.