Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008554

Physical Location & Seq

Genomic Coordinates Reverse (-)

26441090 .. 26444203

Gene Structure 3114 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1157200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1812 bp

ATGGGAATCTCAGTGCCCAGCTTTTTCAAAGAGCGAAAGTTCCCTTTCGTTTTCACACTCTTACTCCCACTCGCCTGCATCACCGTCCTCCTCTTCACCAACATCACCACCAACGCCCCTTACCCTCTCTTCTTCTACTCCGATCTCCAGACCTCCCCCTCCTCCTCCCAACCCATCTCTCCTCCCGCGCCCTCCAACCCCGCCGGAGAGCCGCCCCAAGGGGACGAACTCGTTTGGGAGGAACCCACTTTCCCTGTTTCTGGGATTCCCAATTGGAACCTCTGTACGGGTCCGGTGGCCGTCGATTACATTCCGTGCTTGGACAATTCCAAGGCGATCAAGGCGTTGAAGTCGCGGCGGCATATGGAGCACCGCGAGCGCCATTGCCCCCAGCCGCCGCCGCGGTGCCTGGAGCCGCTTTCCAAAGGGTACAAGGTGCCGGTTCCGTGGCCTCAGAGCAGGGACATGAAGATATGGTATGACAACGTTCCTCACCCGAAGCTCGTCGAGTACAAGAAGGACCAAAACTGGGTGAAGAAGTCCGATGACTATCTCATTTTCCCCGGAGGCGGTACTCAGTTTAAGGATGGAGTTGATCACTACACCGATTTCATCGAAAAGACTTTACCGGTCATTGAATGGGGGAGGCACGTCAGGGTTGTTTTGGACGTTGGCTGCGGTGTTGCTAGCTTTGGCGGCTATTTGCTGGATAAAGATGTCATTACCATGTCATTTGCCCCGAAAGATGAACACGAAGCTCAGATACAGTTTGCTCTGGAAAGGGGAATTCCTGCTACTCTCTCAGTGATTGGAACGCAAAGGCTGACATTTCCGGACAATGCATTTGATTTGATTCACTGTGCAAGGTGCCGGGTTCACTGGGATGCAAATGGAGGGAAACCGCTACTGGAGCTCAACAGGGTTCTTCGGCCTGGGGGGTTTTTCGTATGGTCTGCTACGCCAGTCTATCGCGACAATGAAAGAGATACAAGTGTATGGAAATCAATGGAGGCTCTGACAAAATCAATGTGCTGGGACGTCGTTGCTAAGACTGTCGATTCAACTGGAATCGGGCTTGTAATATATCGGAAGCCTATTGCCTTTGTCCGATATTTTAAACGTAAAGAAAACTATCCACCTCTTTGTGGTCCTGCAGAGAGGAAAGTAGGTGCATGGTATGCGCCGCTCACTGCATGTCTTGCCCCGCTACCTCGCTATAGCTGGCCCTTGCCGTGGCCAAACAGACTTACCAGTAAACCTCCAAGCCTAATACGTGACCCAGAAGGGGAGGAAATGTTCTACAAGGACACCATTCACTGGACTGCGCTTGTCTCAGATGCCTATCAGAAAAATGCTGCTATAAATTGGTCGACTGTGCGGAATGTAATGGATATGAATGCTGGTTTTGGAGGATTCGCTGCTGCACTTACAGATCAACAACTCTGGGTAATGAACATCGTTCCCATTCATCTGCCAAATACTCTTCCAGCTATTTTTGACAGAGGGTTGATCGGAATATACCATGACTGGTGCGAGTCTTTGAATACCTACCCTAGAACATATGATCTGCTGCATTCCAGTTTCCTCTTCGAAAAACTTCCAGACAGATGCGACATTGTAGATGTCGTGGTGGAGATGGATCGCATATTAAGGCCCGGTGGATATCTAGTGGTTCAGGACACAATGGAAGTGATAGACAAGCTGAGACCAATTCTGCAGTTGCTTCACTGGTCTACAGCCCTCTACCGGGATCAGTTTCTTTTCGGTAAGAAAGGTTTCTGGCGTCCATCAAGAGCTGCAAGTAAATCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

604

Amino Acids

68.59

Weight (kDa)

8.11

Isoelectric Point (pI)

40.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	102 - 584	4.5e-206	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25	PF13649	221 - 313	1.3e-07	Methyltransferase domain
Methyltransf_11	PF08241	222 - 316	2.4e-08	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40280
fragaria_vesca	FvH4_7g24980
malus_domestica	MD01G1157200.v1.1 MD07G1226000.v1.1
prunus_persica	Prupe.2G255100_v2.0.a1 Prupe.2G255100_v2.0.a1 Prupe.2G255100_v2.0.a1
pyrus_communis	pycom01g17400 pycom03g12170 pycom07g20440
rosa_chinensis	RchiOBHm_Chr1g0370901
rosa_laevigata	RLG00000027000
rosa_multiflora	Rmu_co8467341.1_g000001 Rmu_sc0001018.1_g000001 Rmu_sc0022073.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286140
rosa_rugosa	Rorug01G0360100 Rorug01G0360100
rosa_samantha	Rh1AG370100 Rh1BG333700 Rh1CG347000 Rh1DG364900
rosa_wichuraiana	Rw1G032570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1041
AccB1I	GGYRCC	3 cut(s)	405, 436, 867
AccB7I	CCANNNNNTGG	1 cut(s)	1317
AccBSI	CCGCTC	1 cut(s)	1186
AccI	GTMKAC	2 cut(s)	1370, 1733
AccII	CGCG	5 cut(s)	188, 355, 375, 403, 972
AccIII	TCCGGA	1 cut(s)	832
AclI	AACGTT	1 cut(s)	486
AclWI	GGATC	2 cut(s)	1647, 1758
AcoI	YGGCCR	2 cut(s)	297, 1235
AcsI	RAATTY	1 cut(s)	786
AcyI	GRCGYC	2 cut(s)	1038, 1783
AfaI	GTAC	4 cut(s)	286, 431, 512, 574
AgeI	ACCGGT	1 cut(s)	628
AgsI	TTSAA	5 cut(s)	28, 349, 638, 1062, 1543
AjiI	CACGTC	1 cut(s)	652
AjnI	CCWGG	2 cut(s)	408, 931
AloI	GAACNNNNNNTCC	4 cut(s)	234, 266, 1280, 1312
AluBI	AGCT	9 cut(s)	21, 502, 690, 758, 913, 1221, 1490, 1702, 1796
AluI	AGCT	9 cut(s)	21, 502, 690, 758, 913, 1221, 1490, 1702, 1796
Alw21I	GWGCWC	2 cut(s)	372, 915
Alw26I	GTCTC	2 cut(s)	1336, 1699
AlwI	GGATC	2 cut(s)	1647, 1758
Aor13HI	TCCGGA	1 cut(s)	832
AoxI	GGCC	6 cut(s)	297, 449, 929, 1223, 1235, 1652
ApeKI	GCWGC	6 cut(s)	675, 1355, 1418, 1421, 1570, 1796
ApoI	RAATTY	1 cut(s)	786
ArsI	GACNNNNNNTTYG	2 cut(s)	827, 859
AsiGI	ACCGGT	1 cut(s)	628
Asp700I	GAANNNNTTC	2 cut(s)	1596, 1775
AspLEI	GCGC	4 cut(s)	190, 381, 1183, 1327
AspS9I	GGNCC	5 cut(s)	290, 520, 1148, 1224, 1653
AsuC2I	CCSGG	4 cut(s)	564, 872, 1656, 1748
AsuHPI	GGTGA	5 cut(s)	73, 88, 97, 485, 544
AsuII	TTCGAA	1 cut(s)	1590
AsuNHI	GCTAGC	1 cut(s)	686
AvaII	GGWCC	3 cut(s)	290, 520, 1148
BaeGI	GKGCMC	1 cut(s)	18
BaeI	ACNNNNGTAYC	2 cut(s)	978, 1011
BalI	TGGCCA	1 cut(s)	1237
BanI	GGYRCC	3 cut(s)	405, 436, 867
BanII	GRGCYC	1 cut(s)	915
BarI	GAAGNNNNNNTAC	2 cut(s)	747, 779
Bbv12I	GWGCWC	2 cut(s)	372, 915
BbvI	GCAGC	6 cut(s)	662, 1342, 1405, 1408, 1557, 1783
BccI	CCATC	4 cut(s)	182, 581, 1630, 1795
BceAI	ACGGC	2 cut(s)	284, 1216
BciT130I	CCWGG	2 cut(s)	410, 933
BclI	TGATCA	1 cut(s)	595
BcnI	CCSGG	4 cut(s)	564, 872, 1656, 1748
BcoDI	GTCTC	2 cut(s)	1336, 1699
BfaI	CTAG	3 cut(s)	687, 1554, 1667
BfmI	CTRYAG	4 cut(s)	1152, 1216, 1715, 1734
BfoI	RGCGCY	1 cut(s)	382
Bme1390I	CCNGG	6 cut(s)	410, 564, 872, 933, 1656, 1748
Bme18I	GGWCC	3 cut(s)	290, 520, 1148
BmgBI	CACGTC	1 cut(s)	652
BmgT120I	GGNCC	5 cut(s)	290, 520, 1148, 1224, 1653
BmiI	GGNNCC	8 cut(s)	243, 278, 291, 407, 414, 438, 444, 869
BmrFI	CCNGG	6 cut(s)	410, 564, 872, 933, 1656, 1748
BmrI	ACTGGG	2 cut(s)	538, 889
BmsI	GCATC	4 cut(s)	87, 874, 1327, 1598
BmtI	GCTAGC	1 cut(s)	690
BmuI	ACTGGG	2 cut(s)	538, 889
BpmI	CTGGAG	3 cut(s)	131, 431, 929
Bpu14I	TTCGAA	1 cut(s)	1590
BpuMI	CCSGG	4 cut(s)	564, 872, 1656, 1748
BsaAI	YACGTR	1 cut(s)	1274
BsaBI	GATNNNNATC	2 cut(s)	549, 1341
BsaHI	GRCGYC	2 cut(s)	1038, 1783
BsaI	GGTCTC	1 cut(s)	1699
BsaJI	CCNNGG	7 cut(s)	217, 330, 401, 446, 562, 932, 1232
BsaWI	WCCGGW	3 cut(s)	292, 628, 832
BsaXI	ACNNNNNCTCC	4 cut(s)	48, 78, 1280, 1310
Bse118I	RCCGGY	2 cut(s)	439, 628
Bse3DI	GCAATG	1 cut(s)	382
Bse8I	GATNNNNATC	2 cut(s)	549, 1341
BseAI	TCCGGA	1 cut(s)	832
BseBI	CCWGG	2 cut(s)	410, 933
BseDI	CCNNGG	7 cut(s)	217, 330, 401, 446, 562, 932, 1232
BseGI	GGATG	2 cut(s)	592, 889
BseJI	GATNNNNATC	2 cut(s)	549, 1341
BseMI	GCAATG	1 cut(s)	382
BseMII	CTCAG	7 cut(s)	24, 467, 590, 773, 816, 1347, 1694
BseRI	GAGGAG	4 cut(s)	80, 151, 154, 171
BseSI	GKGCMC	1 cut(s)	18
BseXI	GCAGC	6 cut(s)	662, 1342, 1405, 1408, 1557, 1783
BseYI	CCCAGC	3 cut(s)	17, 390, 1032
BsgI	GTGCAG	1 cut(s)	1407
Bsh1236I	CGCG	5 cut(s)	188, 355, 375, 403, 972
BshFI	GGCC	6 cut(s)	299, 451, 931, 1225, 1237, 1654
BshNI	GGYRCC	3 cut(s)	405, 436, 867
BshTI	ACCGGT	1 cut(s)	628
BsiHKAI	GWGCWC	2 cut(s)	372, 915
BsiSI	CCGG	9 cut(s)	204, 293, 440, 564, 629, 833, 871, 1656, 1747
BslFI	GGGAC	3 cut(s)	236, 476, 1049
BsmAI	GTCTC	2 cut(s)	1336, 1699
BsmFI	GGGAC	3 cut(s)	236, 476, 1049
BsmI	GAATGC	2 cut(s)	1402, 1573
BsnI	GGCC	6 cut(s)	299, 451, 931, 1225, 1237, 1654
Bso31I	GGTCTC	1 cut(s)	1699
Bsp119I	TTCGAA	1 cut(s)	1590
Bsp1286I	GDGCHC	3 cut(s)	18, 372, 915
Bsp13I	TCCGGA	1 cut(s)	832
Bsp143I	GATC	8 cut(s)	142, 336, 595, 1432, 1509, 1564, 1639, 1750
Bsp68I	TCGCGA	1 cut(s)	972
BspANI	GGCC	6 cut(s)	299, 451, 931, 1225, 1237, 1654
BspCNI	CTCAG	7 cut(s)	23, 466, 589, 772, 815, 1346, 1695
BspEI	TCCGGA	1 cut(s)	832
BspFNI	CGCG	5 cut(s)	188, 355, 375, 403, 972
BspHI	TCATGA	1 cut(s)	1808
BspLI	GGNNCC	8 cut(s)	243, 278, 291, 407, 414, 438, 444, 869
BspMAI	CTGCAG	2 cut(s)	1156, 1719
BspOI	GCTAGC	1 cut(s)	690
BspPI	GGATC	2 cut(s)	1647, 1758
BspT104I	TTCGAA	1 cut(s)	1590
BspT107I	GGYRCC	3 cut(s)	405, 436, 867
BspTNI	GGTCTC	1 cut(s)	1699
BsrBI	CCGCTC	1 cut(s)	1186
BsrDI	GCAATG	1 cut(s)	382
BsrFI	RCCGGY	2 cut(s)	439, 628
BssAI	RCCGGY	2 cut(s)	439, 628
BssECI	CCNNGG	7 cut(s)	217, 330, 401, 446, 562, 932, 1232
BssMI	GATC	8 cut(s)	142, 336, 595, 1432, 1509, 1564, 1639, 1750
BssNI	GRCGYC	2 cut(s)	1038, 1783
BssT1I	CCWWGG	2 cut(s)	217, 330
Bst2UI	CCWGG	2 cut(s)	410, 933
Bst4CI	ACNGT	5 cut(s)	85, 768, 860, 1054, 1375
Bst6I	CTCTTC	4 cut(s)	98, 134, 1488, 1592
BstACI	GRCGYC	2 cut(s)	1038, 1783
BstAPI	GCANNNNNTGC	2 cut(s)	1178, 1199
BstBAI	YACGTR	1 cut(s)	1274
BstBI	TTCGAA	1 cut(s)	1590
BstC8I	GCNNGC	4 cut(s)	76, 377, 688, 1223
BstDEI	CTNAG	8 cut(s)	10, 453, 576, 759, 802, 1047, 1333, 1703
BstDSI	CCRYGG	3 cut(s)	401, 446, 1232
BstF5I	GGATG	2 cut(s)	592, 889
BstFNI	CGCG	5 cut(s)	188, 355, 375, 403, 972
BstH2I	RGCGCY	1 cut(s)	382
BstHHI	GCGC	4 cut(s)	190, 381, 1183, 1327
BstKTI	GATC	8 cut(s)	145, 339, 598, 1435, 1512, 1567, 1642, 1753
BstMAI	GTCTC	2 cut(s)	1336, 1699
BstMBI	GATC	8 cut(s)	142, 336, 595, 1432, 1509, 1564, 1639, 1750
BstMWI	GCNNNNNNNGC	8 cut(s)	341, 367, 376, 400, 696, 910, 1178, 1199
BstNI	CCWGG	2 cut(s)	410, 933
BstNSI	RCATGY	1 cut(s)	1197
BstSCI	CCNGG	6 cut(s)	408, 562, 870, 931, 1654, 1746
BstSFI	CTRYAG	4 cut(s)	1152, 1216, 1715, 1734
BstSLI	GKGCMC	1 cut(s)	18
BstUI	CGCG	5 cut(s)	188, 355, 375, 403, 972
BstV1I	GCAGC	6 cut(s)	662, 1342, 1405, 1408, 1557, 1783
BsuRI	GGCC	6 cut(s)	299, 451, 931, 1225, 1237, 1654
BtgI	CCRYGG	3 cut(s)	401, 446, 1232
BtrI	CACGTC	1 cut(s)	652
BtsCI	GGATG	2 cut(s)	592, 889
BtsI	GCAGTG	1 cut(s)	1188
BtsIMutI	CAGTG	7 cut(s)	18, 810, 856, 877, 1188, 1315, 1726
BtuMI	TCGCGA	1 cut(s)	972
Cac8I	GCNNGC	4 cut(s)	76, 377, 688, 1223
CciI	TCATGA	1 cut(s)	1808
CfoI	GCGC	4 cut(s)	190, 381, 1183, 1327
Cfr10I	RCCGGY	2 cut(s)	439, 628
Cfr13I	GGNCC	5 cut(s)	290, 520, 1148, 1224, 1653
Cfr42I	CCGCGG	1 cut(s)	404
CseI	GACGC	1 cut(s)	1772
Csp6I	GTAC	4 cut(s)	285, 430, 511, 573
CspAI	ACCGGT	1 cut(s)	628
CviAII	CATG	6 cut(s)	466, 727, 1173, 1194, 1523, 1809
CviQI	GTAC	4 cut(s)	285, 430, 511, 573
DdeI	CTNAG	8 cut(s)	10, 453, 576, 759, 802, 1047, 1333, 1703
DpnI	GATC	8 cut(s)	144, 338, 597, 1434, 1511, 1566, 1641, 1752
DpnII	GATC	8 cut(s)	142, 336, 595, 1432, 1509, 1564, 1639, 1750
DraI	TTTAAA	1 cut(s)	1117
EaeI	YGGCCR	2 cut(s)	297, 1235
Eam1104I	CTCTTC	4 cut(s)	98, 134, 1488, 1592
EarI	CTCTTC	4 cut(s)	98, 134, 1488, 1592
Ecl136II	GAGCTC	1 cut(s)	913
Eco130I	CCWWGG	2 cut(s)	217, 330
Eco24I	GRGCYC	1 cut(s)	915
Eco31I	GGTCTC	1 cut(s)	1699
Eco32I	GATATC	1 cut(s)	1664
Eco47I	GGWCC	3 cut(s)	290, 520, 1148
Eco53kI	GAGCTC	1 cut(s)	913
EcoICRI	GAGCTC	1 cut(s)	913
EcoRI	GAATTC	1 cut(s)	786
EcoRII	CCWGG	2 cut(s)	408, 931
EcoRV	GATATC	1 cut(s)	1664
EcoT14I	CCWWGG	2 cut(s)	217, 330
EcoT22I	ATGCAT	1 cut(s)	844
EcoT38I	GRGCYC	1 cut(s)	915
ErhI	CCWWGG	2 cut(s)	217, 330
FaeI	CATG	6 cut(s)	469, 730, 1176, 1197, 1526, 1812
FaqI	GGGAC	3 cut(s)	236, 476, 1049
FatI	CATG	6 cut(s)	465, 726, 1172, 1193, 1522, 1808
FauI	CCCGC	3 cut(s)	193, 208, 1212
FauNDI	CATATG	2 cut(s)	363, 1561
FbaI	TGATCA	1 cut(s)	595
FblI	GTMKAC	2 cut(s)	1370, 1733
FokI	GGATG	2 cut(s)	599, 896
FriOI	GRGCYC	1 cut(s)	915
FspBI	CTAG	3 cut(s)	687, 1554, 1667
GlaI	GCGC	4 cut(s)	189, 380, 1182, 1326
GsaI	CCCAGC	3 cut(s)	21, 394, 1036
GsuI	CTGGAG	3 cut(s)	131, 431, 929
HaeII	RGCGCY	1 cut(s)	382
HaeIII	GGCC	6 cut(s)	299, 451, 931, 1225, 1237, 1654
HapII	CCGG	9 cut(s)	204, 293, 440, 564, 629, 833, 871, 1656, 1747
HgaI	GACGC	1 cut(s)	1772
HhaI	GCGC	4 cut(s)	190, 381, 1183, 1327
Hin1I	GRCGYC	2 cut(s)	1038, 1783
Hin1II	CATG	6 cut(s)	469, 730, 1176, 1197, 1526, 1812
Hin6I	GCGC	4 cut(s)	188, 379, 1181, 1325
HinP1I	GCGC	4 cut(s)	188, 379, 1181, 1325
HincII	GTYRAC	1 cut(s)	1371
HindII	GTYRAC	1 cut(s)	1371
HinfI	GANTC	7 cut(s)	6, 265, 853, 1058, 1068, 1413, 1535
HpaII	CCGG	9 cut(s)	204, 293, 440, 564, 629, 833, 871, 1656, 1747
HphI	GGTGA	5 cut(s)	73, 88, 97, 485, 544
Hpy166II	GTNNAC	4 cut(s)	877, 1256, 1371, 1734
Hpy188III	TCNNGA	8 cut(s)	148, 776, 833, 971, 1601, 1676, 1791, 1809
Hpy8I	GTNNAC	4 cut(s)	877, 1256, 1371, 1734
Hpy99I	CGWCG	3 cut(s)	305, 509, 1043
HpyAV	CCTTC	2 cut(s)	511, 1277
HpyCH4III	ACNGT	5 cut(s)	85, 768, 860, 1054, 1375
HpyCH4IV	ACGT	6 cut(s)	486, 651, 669, 1038, 1120, 1273
HpyF10VI	GCNNNNNNNGC	8 cut(s)	341, 367, 376, 400, 696, 910, 1178, 1199
HpyF3I	CTNAG	8 cut(s)	10, 453, 576, 759, 802, 1047, 1333, 1703
HpySE526I	ACGT	6 cut(s)	486, 651, 669, 1038, 1120, 1273
Hsp92I	GRCGYC	2 cut(s)	1038, 1783
Hsp92II	CATG	6 cut(s)	469, 730, 1176, 1197, 1526, 1812
HspAI	GCGC	4 cut(s)	188, 379, 1181, 1325
Kpn2I	TCCGGA	1 cut(s)	832
Ksp22I	TGATCA	1 cut(s)	595
KspI	CCGCGG	1 cut(s)	404
Kzo9I	GATC	8 cut(s)	142, 336, 595, 1432, 1509, 1564, 1639, 1750
LmnI	GCTCC	3 cut(s)	367, 412, 910
Lsp1109I	GCAGC	6 cut(s)	662, 1342, 1405, 1408, 1557, 1783
LweI	GCATC	4 cut(s)	87, 874, 1327, 1598
MaeI	CTAG	3 cut(s)	687, 1554, 1667
MaeII	ACGT	6 cut(s)	486, 651, 669, 1038, 1120, 1273
MaeIII	GTNAC	1 cut(s)	1274
MalI	GATC	8 cut(s)	144, 338, 597, 1434, 1511, 1566, 1641, 1752
MbiI	CCGCTC	1 cut(s)	1186
MboI	GATC	8 cut(s)	142, 336, 595, 1432, 1509, 1564, 1639, 1750
MboII	GAAGA	8 cut(s)	85, 121, 124, 481, 547, 917, 1475, 1579
MfeI	CAATTG	1 cut(s)	271
MhlI	GDGCHC	3 cut(s)	18, 372, 915
MlsI	TGGCCA	1 cut(s)	1237
MluCI	AATT	5 cut(s)	271, 325, 786, 1363, 1710
MluNI	TGGCCA	1 cut(s)	1237
MlyI	GAGTC	1 cut(s)	1544
MmeI	TCCRAC	1 cut(s)	219
Mox20I	TGGCCA	1 cut(s)	1237
Mph1103I	ATGCAT	1 cut(s)	844
MroI	TCCGGA	1 cut(s)	832
MroXI	GAANNNNTTC	2 cut(s)	1596, 1775
MscI	TGGCCA	1 cut(s)	1237
MseI	TTAA	3 cut(s)	582, 1116, 1649
MslI	CAYNNNNRTG	2 cut(s)	725, 882
Msp20I	TGGCCA	1 cut(s)	1237
MspA1I	CMGCKG	1 cut(s)	403
MspI	CCGG	9 cut(s)	204, 293, 440, 564, 629, 833, 871, 1656, 1747
MspR9I	CCNGG	6 cut(s)	410, 564, 872, 933, 1656, 1748
MunI	CAATTG	1 cut(s)	271
Mva1269I	GAATGC	2 cut(s)	1402, 1573
MvaI	CCWGG	2 cut(s)	410, 933
MvnI	CGCG	5 cut(s)	188, 355, 375, 403, 972
MwoI	GCNNNNNNNGC	8 cut(s)	341, 367, 376, 400, 696, 910, 1178, 1199
NciI	CCSGG	4 cut(s)	564, 872, 1656, 1748
NdeI	CATATG	2 cut(s)	363, 1561
NdeII	GATC	8 cut(s)	142, 336, 595, 1432, 1509, 1564, 1639, 1750
NheI	GCTAGC	1 cut(s)	686
NlaIII	CATG	6 cut(s)	469, 730, 1176, 1197, 1526, 1812
NlaIV	GGNNCC	8 cut(s)	243, 278, 291, 407, 414, 438, 444, 869
NmuCI	GTSAC	1 cut(s)	1274
NruI	TCGCGA	1 cut(s)	972
NsiI	ATGCAT	1 cut(s)	844
NspI	RCATGY	1 cut(s)	1197
NspV	TTCGAA	1 cut(s)	1590
PagI	TCATGA	1 cut(s)	1808
PctI	GAATGC	2 cut(s)	1402, 1573
PdmI	GAANNNNTTC	2 cut(s)	1596, 1775
PfeI	GAWTC	6 cut(s)	6, 265, 853, 1058, 1068, 1413
PflMI	CCANNNNNTGG	1 cut(s)	1317
PinAI	ACCGGT	1 cut(s)	628
PleI	GAGTC	1 cut(s)	1543
PpsI	GAGTC	1 cut(s)	1543
Ppu21I	YACGTR	1 cut(s)	1274
Psp124BI	GAGCTC	1 cut(s)	915
Psp1406I	AACGTT	1 cut(s)	486
Psp6I	CCWGG	2 cut(s)	408, 931
PspFI	CCCAGC	3 cut(s)	17, 390, 1032
PspGI	CCWGG	2 cut(s)	408, 931
PspN4I	GGNNCC	8 cut(s)	243, 278, 291, 407, 414, 438, 444, 869
PspPI	GGNCC	5 cut(s)	290, 520, 1148, 1224, 1653
PstI	CTGCAG	2 cut(s)	1156, 1719
RruI	TCGCGA	1 cut(s)	972
RsaI	GTAC	4 cut(s)	286, 431, 512, 574
RsaNI	GTAC	4 cut(s)	285, 430, 511, 573
RseI	CAYNNNNRTG	2 cut(s)	725, 882
SacI	GAGCTC	1 cut(s)	915
SacII	CCGCGG	1 cut(s)	404
SalI	GTCGAC	1 cut(s)	1369
SaqAI	TTAA	3 cut(s)	582, 1116, 1649
Sau3AI	GATC	8 cut(s)	142, 336, 595, 1432, 1509, 1564, 1639, 1750
Sau96I	GGNCC	5 cut(s)	290, 520, 1148, 1224, 1653
SchI	GAGTC	1 cut(s)	1544
ScrFI	CCNGG	6 cut(s)	410, 564, 872, 933, 1656, 1748
SduI	GDGCHC	3 cut(s)	18, 372, 915
SfaNI	GCATC	4 cut(s)	87, 874, 1327, 1598
SfcI	CTRYAG	4 cut(s)	1152, 1216, 1715, 1734
Sfr303I	CCGCGG	1 cut(s)	404
SfuI	TTCGAA	1 cut(s)	1590
SgrBI	CCGCGG	1 cut(s)	404
SinI	GGWCC	3 cut(s)	290, 520, 1148
SmiMI	CAYNNNNRTG	2 cut(s)	725, 882
Sse9I	AATT	5 cut(s)	271, 325, 786, 1363, 1710
SspMI	CTAG	3 cut(s)	687, 1554, 1667
SstI	GAGCTC	1 cut(s)	915
StyD4I	CCNGG	6 cut(s)	408, 562, 870, 931, 1654, 1746
StyI	CCWWGG	2 cut(s)	217, 330
TaaI	ACNGT	5 cut(s)	85, 768, 860, 1054, 1375
TaiI	ACGT	6 cut(s)	489, 654, 672, 1041, 1123, 1276
TaqI	TCGA	6 cut(s)	303, 507, 615, 1056, 1370, 1590
TasI	AATT	5 cut(s)	271, 325, 786, 1363, 1710
TatI	WGTACW	1 cut(s)	510
TauI	GCSGC	9 cut(s)	214, 358, 361, 397, 400, 403, 418, 699, 1186
TfiI	GAWTC	6 cut(s)	6, 265, 853, 1058, 1068, 1413
Tru1I	TTAA	3 cut(s)	582, 1116, 1649
Tru9I	TTAA	3 cut(s)	582, 1116, 1649
TscAI	CASTG	7 cut(s)	18, 810, 863, 884, 1195, 1322, 1733
TseFI	GTSAC	1 cut(s)	1274
TseI	GCWGC	6 cut(s)	675, 1355, 1418, 1421, 1570, 1796
Tsp45I	GTSAC	1 cut(s)	1274
TspDTI	ATGAA	7 cut(s)	482, 601, 762, 993, 1409, 1457, 1466
TspGWI	ACGGA	2 cut(s)	303, 435
TspRI	CASTG	7 cut(s)	18, 810, 863, 884, 1195, 1322, 1733
Van91I	CCANNNNNTGG	1 cut(s)	1317
VpaK11BI	GGWCC	3 cut(s)	290, 520, 1148
XapI	RAATTY	1 cut(s)	786
XceI	RCATGY	1 cut(s)	1197
XmiI	GTMKAC	2 cut(s)	1370, 1733
XmnI	GAANNNNTTC	2 cut(s)	1596, 1775
XspI	CTAG	3 cut(s)	687, 1554, 1667
ZraI	GACGTC	1 cut(s)	1039
Zsp2I	ATGCAT	1 cut(s)	844

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.