Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008554

Physical Location & Seq

Genomic Coordinates Reverse (-)

30197796 .. 30203006

Gene Structure 5211 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1226000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1791 bp

ATGGGAATCTCAGTCTCCAGCTTTTTCAAAGAGCGAAAATTCCTCTTCGTTTTCACACTCTCACTCCTACTCGTCTGCCTCACAATCCTCCTCTTCACCAGCACCAGCACCCCTTACCCTCTCTTCTTCTACTCCGATCTCCAAACCTCCCAACCCATCTCCCCTTCCGCCCCCTCCAACCCCTCCGGAGCGCCGACCCAAGTAGACGACCTCGTTTGGGAGGAACCCTCTTTCCCTGTTTCTGGGAATTCGAATTGGAACCTCTGTACGGGTCCGGTGGCGGTCGATTACATTCCGTGCTTGGACAATTTTAAGGCGATCAAGGCGTTGCAGTCGCGGCGGCATATGGAGCACCGCGAGCGGCATTGCCCCCAGCCGCCCCCACGGTGCCTGGAGCCGCTTCCCAAAGGGTACAAGGTGCCGGTTTCGTGGCCTCAGAGCAGGGACATGATATGGTATGACAATGTTCCTCACCCGAAGCTCGTCGAGTACAAGAAGGACCAAAATTGGGTGAAGAAGTCTGATGACCATCTTATTTTCCCCGGGGGTGGTACTCAGTTCAAGGAAGGAGTTGATCACTACACTGATTTCATCGAAAAGACTTTGCCGGTTATTGAATGGGGGAGGCATGTAAGGGTTGTTTTAGATGTTGGTTGTGGTGTTGCTAGCTTTGGTGGCTATTTGCTGGATAAAAATGTTATTACCATGTCATTTGCCCCGAAAGATGAACATGAAGCTCAGATACAGTTTGCTCTTGAAAGGGGGATTCCTGCTACTCTCTCAGTGATTGGAACGCAAAGGCTGACATTTCCGAACGATGCATTTGATTTAATTCATTGTGCAAGGTGTCGGGTTCACTGGGATGCCGATGGAGGGAAACCACTACTGGAGCTCAACAGGGTTCTTCGGCCTGGGGGTTTTTTCGTATGGTCAGCTACACCAGTTTATCGCGACAATGAAAGAGATACAAGTGTATGGAAATCAATGGAGGCTCTGACAAAATCAATGTGCTGGGACGTTGTTGCTAAGACTGTCGATTCAACTGGAATTGGGCTTGTAATATATCAGAAGCCTATCTCCTTTGCCCGATATTTCAAACGCAAAGAAAACAATCCACCTGTTTGTGGTCCTAAAGAGAGGAAAGTAGGTGCATGGTACGCGCCTCTCACTGCTTGTCTTACCCCACTACCTCGCTATGGCTGGCCCTTGCCCTGGCCTATCAGACTTACCGGTAAACCTCCAAGCCTAAATCATGACCCGGAAGGGGAGGAATTGTTCTACAAGGACACCATTCAATGGACTGCACTTGTGTCAGATGCCTATAAAAAAAATGCTGCTATAAATTGGTCGACTGTGCGGAATGTAATGGATATGAATGCTGGTTTTGGAGGGTTCGCTGCAGCACTTACCGATCAACAGCTCTGGGTAATGAATGTCGTCCCCATTCATCTACCCAATACTCTGCCGGTTATTTTTGACAGAGGGTTGATCGGAATATACCATGACTGGTGCGAGTCTTTGAATACCTACCCTAGAACCTATGATCTGCTGCATTCCAATTTCCTCTTCGAAAAACTTCCAGACAGATGCGACATTGTAGATGTCGTGGTGGAGATGGATCGCGTATTAAGGCCCGGTGGATATCTAGTGGTTCGGGACACAGTTGAAGTGATCGACAAGCTGAGACCGATTCTGCAATCGCTTCACTGGTCTACAGCCCTCTACCGAGATCAGTTTCTTTTCGGTAAGAAAGGTCTCTGGCGTCCTTCGAGAGGTGCAAGTAACTCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0012505 GO:0016740 GO:0016741 GO:0031410 GO:0031982 GO:0031984 GO:0032259 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0097708 GO:0098791

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Methyltransf_29

Protein Analysis

597

Amino Acids

67.72

Weight (kDa)

7.88

Isoelectric Point (pI)

35.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Methyltransf_29	PF03141	96 - 577	2e-210	Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_25	PF13649	214 - 306	1.7e-07	Methyltransferase domain
Methyltransf_11	PF08241	215 - 309	1.1e-07	Methyltransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008554)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40280
fragaria_vesca	FvH4_7g24980
malus_domestica	MD01G1157200.v1.1 MD07G1226000.v1.1
prunus_persica	Prupe.2G255100_v2.0.a1 Prupe.2G255100_v2.0.a1 Prupe.2G255100_v2.0.a1
pyrus_communis	pycom01g17400 pycom03g12170 pycom07g20440
rosa_chinensis	RchiOBHm_Chr1g0370901
rosa_laevigata	RLG00000027000
rosa_multiflora	Rmu_co8467341.1_g000001 Rmu_sc0001018.1_g000001 Rmu_sc0022073.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286140
rosa_rugosa	Rorug01G0360100 Rorug01G0360100
rosa_samantha	Rh1AG370100 Rh1BG333700 Rh1CG347000 Rh1DG364900
rosa_wichuraiana	Rw1G032570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	387, 418
AccB7I	CCANNNNNTGG	1 cut(s)	1296
AccBSI	CCGCTC	1 cut(s)	361
AccI	GTMKAC	3 cut(s)	204, 1349, 1712
AccII	CGCG	5 cut(s)	337, 357, 951, 1160, 1623
AccIII	TCCGGA	1 cut(s)	185
AciI	CCGC	9 cut(s)	168, 281, 337, 340, 355, 361, 377, 398, 1357
AclWI	GGATC	1 cut(s)	1626
AcsI	RAATTY	2 cut(s)	38, 247
AcyI	GRCGYC	1 cut(s)	1762
AfaI	GTAC	5 cut(s)	268, 413, 491, 553, 1157
AgeI	ACCGGT	1 cut(s)	1229
AgsI	TTSAA	9 cut(s)	28, 562, 617, 758, 1041, 1096, 1295, 1522, 1667
AjnI	CCWGG	3 cut(s)	390, 910, 1211
AjuI	GAANNNNNNNTTGG	2 cut(s)	1550, 1582
AloI	GAACNNNNNNTCC	4 cut(s)	216, 248, 1259, 1291
AluBI	AGCT	8 cut(s)	21, 481, 669, 737, 892, 935, 1420, 1681
AluI	AGCT	8 cut(s)	21, 481, 669, 737, 892, 935, 1420, 1681
Alw21I	GWGCWC	2 cut(s)	354, 894
Alw26I	GTCTC	3 cut(s)	19, 1678, 1760
AlwI	GGATC	1 cut(s)	1626
Ama87I	CYCGRG	1 cut(s)	542
Aor13HI	TCCGGA	1 cut(s)	185
AoxI	GGCC	5 cut(s)	431, 908, 1202, 1214, 1631
ApeKI	GCWGC	4 cut(s)	1334, 1397, 1400, 1549
ApoI	RAATTY	2 cut(s)	38, 247
AsiGI	ACCGGT	1 cut(s)	1229
Asp700I	GAANNNNTTC	1 cut(s)	1575
AspLEI	GCGC	2 cut(s)	193, 1162
AspS9I	GGNCC	5 cut(s)	272, 499, 1127, 1203, 1632
AsuC2I	CCSGG	4 cut(s)	543, 544, 1259, 1635
AsuHPI	GGTGA	3 cut(s)	88, 464, 523
AsuII	TTCGAA	2 cut(s)	251, 1569
AsuNHI	GCTAGC	1 cut(s)	665
AvaI	CYCGRG	1 cut(s)	542
AvaII	GGWCC	3 cut(s)	272, 499, 1127
BaeI	ACNNNNGTAYC	2 cut(s)	957, 990
BanI	GGYRCC	2 cut(s)	387, 418
BanII	GRGCYC	1 cut(s)	894
BarI	GAAGNNNNNNTAC	2 cut(s)	726, 758
BauI	CACGAG	1 cut(s)	1786
Bbv12I	GWGCWC	2 cut(s)	354, 894
BbvI	GCAGC	4 cut(s)	1321, 1384, 1412, 1536
BccI	CCATC	4 cut(s)	164, 537, 863, 1609
BcgI	CGANNNNNNTGC	1 cut(s)	1767
BciT130I	CCWGG	3 cut(s)	392, 912, 1213
BclI	TGATCA	1 cut(s)	574
BcnI	CCSGG	4 cut(s)	543, 544, 1259, 1635
BcoDI	GTCTC	3 cut(s)	19, 1678, 1760
BfaI	CTAG	3 cut(s)	666, 1533, 1646
BfmI	CTRYAG	2 cut(s)	1398, 1713
BfoI	RGCGCY	1 cut(s)	194
BisI	GCNGC	9 cut(s)	338, 341, 362, 377, 398, 1335, 1398, 1401, 1550
BlsI	GCNGC	9 cut(s)	339, 342, 363, 378, 399, 1336, 1399, 1402, 1551
Bme1390I	CCNGG	7 cut(s)	392, 543, 544, 912, 1213, 1259, 1635
Bme18I	GGWCC	3 cut(s)	272, 499, 1127
BmeT110I	CYCGRG	1 cut(s)	542
BmgT120I	GGNCC	5 cut(s)	272, 499, 1127, 1203, 1632
BmiI	GGNNCC	6 cut(s)	225, 260, 273, 389, 396, 420
BmrFI	CCNGG	7 cut(s)	392, 543, 544, 912, 1213, 1259, 1635
BmrI	ACTGGG	1 cut(s)	868
BmsI	GCATC	4 cut(s)	808, 853, 1306, 1577
BmtI	GCTAGC	1 cut(s)	669
BmuI	ACTGGG	1 cut(s)	868
BplI	GAGNNNNNCTC	2 cut(s)	212, 244
BpmI	CTGGAG	2 cut(s)	413, 908
Bpu14I	TTCGAA	2 cut(s)	251, 1569
BpuMI	CCSGG	4 cut(s)	543, 544, 1259, 1635
BsaBI	GATNNNNATC	1 cut(s)	528
BsaHI	GRCGYC	1 cut(s)	1762
BsaI	GGTCTC	2 cut(s)	1678, 1760
BsaJI	CCNNGG	6 cut(s)	383, 541, 542, 543, 911, 1211
BsaWI	WCCGGW	3 cut(s)	185, 274, 1229
BsaXI	ACNNNNNCTCC	4 cut(s)	48, 78, 1259, 1289
Bse118I	RCCGGY	4 cut(s)	421, 607, 1229, 1465
Bse1I	ACTGG	6 cut(s)	863, 891, 941, 1048, 1511, 1712
Bse3DI	GCAATG	1 cut(s)	364
Bse8I	GATNNNNATC	1 cut(s)	528
BseAI	TCCGGA	1 cut(s)	185
BseBI	CCWGG	3 cut(s)	392, 912, 1213
BseDI	CCNNGG	6 cut(s)	383, 541, 542, 543, 911, 1211
BseGI	GGATG	1 cut(s)	868
BseJI	GATNNNNATC	1 cut(s)	528
BseMI	GCAATG	1 cut(s)	364
BseMII	CTCAG	6 cut(s)	24, 449, 569, 752, 795, 1673
BseNI	ACTGG	6 cut(s)	863, 891, 941, 1048, 1511, 1712
BseRI	GAGGAG	1 cut(s)	80
BseXI	GCAGC	4 cut(s)	1321, 1384, 1412, 1536
BseYI	CCCAGC	2 cut(s)	372, 1011
BsgI	GTGCAG	1 cut(s)	1287
Bsh1236I	CGCG	5 cut(s)	337, 357, 951, 1160, 1623
Bsh1285I	CGRYCG	1 cut(s)	285
BshFI	GGCC	5 cut(s)	433, 910, 1204, 1216, 1633
BshNI	GGYRCC	2 cut(s)	387, 418
BshTI	ACCGGT	1 cut(s)	1229
BsiEI	CGRYCG	1 cut(s)	285
BsiHKAI	GWGCWC	2 cut(s)	354, 894
BsiHKCI	CYCGRG	1 cut(s)	542
BsiSI	CCGG	9 cut(s)	186, 275, 422, 543, 608, 1230, 1259, 1466, 1635
BslFI	GGGAC	4 cut(s)	458, 1028, 1424, 1670
BsmAI	GTCTC	3 cut(s)	19, 1678, 1760
BsmFI	GGGAC	4 cut(s)	458, 1028, 1424, 1670
BsmI	GAATGC	2 cut(s)	1381, 1552
BsnI	GGCC	5 cut(s)	433, 910, 1204, 1216, 1633
Bso31I	GGTCTC	2 cut(s)	1678, 1760
BsoBI	CYCGRG	1 cut(s)	542
Bsp119I	TTCGAA	2 cut(s)	251, 1569
Bsp1286I	GDGCHC	2 cut(s)	354, 894
Bsp13I	TCCGGA	1 cut(s)	185
Bsp143I	GATC	9 cut(s)	136, 318, 574, 1411, 1488, 1543, 1618, 1671, 1729
Bsp68I	TCGCGA	1 cut(s)	951
BspACI	CCGC	9 cut(s)	168, 281, 337, 340, 355, 361, 377, 398, 1357
BspANI	GGCC	5 cut(s)	433, 910, 1204, 1216, 1633
BspCNI	CTCAG	6 cut(s)	23, 448, 568, 751, 794, 1674
BspEI	TCCGGA	1 cut(s)	185
BspFNI	CGCG	5 cut(s)	337, 357, 951, 1160, 1623
BspHI	TCATGA	1 cut(s)	1252
BspLI	GGNNCC	6 cut(s)	225, 260, 273, 389, 396, 420
BspMAI	CTGCAG	1 cut(s)	1402
BspOI	GCTAGC	1 cut(s)	669
BspPI	GGATC	1 cut(s)	1626
BspT104I	TTCGAA	2 cut(s)	251, 1569
BspT107I	GGYRCC	2 cut(s)	387, 418
BspTNI	GGTCTC	2 cut(s)	1678, 1760
BsrBI	CCGCTC	1 cut(s)	361
BsrDI	GCAATG	1 cut(s)	364
BsrFI	RCCGGY	4 cut(s)	421, 607, 1229, 1465
BsrI	ACTGG	6 cut(s)	863, 891, 941, 1048, 1511, 1712
BssAI	RCCGGY	4 cut(s)	421, 607, 1229, 1465
BssECI	CCNNGG	6 cut(s)	383, 541, 542, 543, 911, 1211
BssMI	GATC	9 cut(s)	136, 318, 574, 1411, 1488, 1543, 1618, 1671, 1729
BssNI	GRCGYC	1 cut(s)	1762
BssSI	CACGAG	1 cut(s)	1786
Bst2BI	CACGAG	1 cut(s)	1786
Bst2UI	CCWGG	3 cut(s)	392, 912, 1213
Bst4CI	ACNGT	5 cut(s)	387, 747, 1033, 1354, 1663
Bst6I	CTCTTC	4 cut(s)	50, 98, 128, 1571
BstACI	GRCGYC	1 cut(s)	1762
BstBI	TTCGAA	2 cut(s)	251, 1569
BstC8I	GCNNGC	3 cut(s)	359, 667, 1202
BstDEI	CTNAG	7 cut(s)	10, 435, 555, 738, 781, 1026, 1682
BstDSI	CCRYGG	1 cut(s)	383
BstENI	CCTNNNNNAGG	1 cut(s)	1770
BstF5I	GGATG	1 cut(s)	868
BstFNI	CGCG	5 cut(s)	337, 357, 951, 1160, 1623
BstH2I	RGCGCY	1 cut(s)	194
BstHHI	GCGC	2 cut(s)	193, 1162
BstKTI	GATC	9 cut(s)	139, 321, 577, 1414, 1491, 1546, 1621, 1674, 1732
BstMAI	GTCTC	3 cut(s)	19, 1678, 1760
BstMBI	GATC	9 cut(s)	136, 318, 574, 1411, 1488, 1543, 1618, 1671, 1729
BstMCI	CGRYCG	1 cut(s)	285
BstMWI	GCNNNNNNNGC	6 cut(s)	323, 337, 349, 358, 675, 1157
BstNI	CCWGG	3 cut(s)	392, 912, 1213
BstNSI	RCATGY	1 cut(s)	632
BstSCI	CCNGG	7 cut(s)	390, 541, 542, 910, 1211, 1257, 1633
BstSFI	CTRYAG	2 cut(s)	1398, 1713
BstUI	CGCG	5 cut(s)	337, 357, 951, 1160, 1623
BstV1I	GCAGC	4 cut(s)	1321, 1384, 1412, 1536
BsuRI	GGCC	5 cut(s)	433, 910, 1204, 1216, 1633
BtgI	CCRYGG	1 cut(s)	383
BtsCI	GGATG	1 cut(s)	868
BtsI	GCAGTG	1 cut(s)	1167
BtsIMutI	CAGTG	5 cut(s)	582, 789, 856, 1167, 1705
BtuMI	TCGCGA	1 cut(s)	951
Cac8I	GCNNGC	3 cut(s)	359, 667, 1202
CciI	TCATGA	1 cut(s)	1252
CfoI	GCGC	2 cut(s)	193, 1162
Cfr10I	RCCGGY	4 cut(s)	421, 607, 1229, 1465
Cfr13I	GGNCC	5 cut(s)	272, 499, 1127, 1203, 1632
Cfr9I	CCCGGG	1 cut(s)	542
CseI	GACGC	1 cut(s)	1751
Csp6I	GTAC	5 cut(s)	267, 412, 490, 552, 1156
CspAI	ACCGGT	1 cut(s)	1229
CviAII	CATG	7 cut(s)	448, 629, 706, 731, 1152, 1253, 1502
CviQI	GTAC	5 cut(s)	267, 412, 490, 552, 1156
DdeI	CTNAG	7 cut(s)	10, 435, 555, 738, 781, 1026, 1682
DpnI	GATC	9 cut(s)	138, 320, 576, 1413, 1490, 1545, 1620, 1673, 1731
DpnII	GATC	9 cut(s)	136, 318, 574, 1411, 1488, 1543, 1618, 1671, 1729
Eam1104I	CTCTTC	4 cut(s)	50, 98, 128, 1571
EarI	CTCTTC	4 cut(s)	50, 98, 128, 1571
EciI	GGCGGA	1 cut(s)	157
Ecl136II	GAGCTC	1 cut(s)	892
Eco24I	GRGCYC	1 cut(s)	894
Eco31I	GGTCTC	2 cut(s)	1678, 1760
Eco32I	GATATC	1 cut(s)	1643
Eco47I	GGWCC	3 cut(s)	272, 499, 1127
Eco53kI	GAGCTC	1 cut(s)	892
Eco88I	CYCGRG	1 cut(s)	542
EcoICRI	GAGCTC	1 cut(s)	892
EcoNI	CCTNNNNNAGG	1 cut(s)	1770
EcoRI	GAATTC	1 cut(s)	247
EcoRII	CCWGG	3 cut(s)	390, 910, 1211
EcoRV	GATATC	1 cut(s)	1643
EcoT22I	ATGCAT	1 cut(s)	823
EcoT38I	GRGCYC	1 cut(s)	894
FaeI	CATG	7 cut(s)	451, 632, 709, 734, 1155, 1256, 1505
FaqI	GGGAC	4 cut(s)	458, 1028, 1424, 1670
FatI	CATG	7 cut(s)	447, 628, 705, 730, 1151, 1252, 1501
FauNDI	CATATG	1 cut(s)	345
FbaI	TGATCA	1 cut(s)	574
FblI	GTMKAC	3 cut(s)	204, 1349, 1712
Fnu4HI	GCNGC	9 cut(s)	338, 341, 362, 377, 398, 1335, 1398, 1401, 1550
FokI	GGATG	1 cut(s)	875
FriOI	GRGCYC	1 cut(s)	894
Fsp4HI	GCNGC	9 cut(s)	338, 341, 362, 377, 398, 1335, 1398, 1401, 1550
FspBI	CTAG	3 cut(s)	666, 1533, 1646
GlaI	GCGC	2 cut(s)	192, 1161
GluI	GCNGC	9 cut(s)	338, 341, 362, 377, 398, 1335, 1398, 1401, 1550
GsaI	CCCAGC	2 cut(s)	376, 1015
GsuI	CTGGAG	2 cut(s)	413, 908
HaeII	RGCGCY	1 cut(s)	194
HaeIII	GGCC	5 cut(s)	433, 910, 1204, 1216, 1633
HapII	CCGG	9 cut(s)	186, 275, 422, 543, 608, 1230, 1259, 1466, 1635
HgaI	GACGC	1 cut(s)	1751
HhaI	GCGC	2 cut(s)	193, 1162
Hin1I	GRCGYC	1 cut(s)	1762
Hin1II	CATG	7 cut(s)	451, 632, 709, 734, 1155, 1256, 1505
Hin6I	GCGC	2 cut(s)	191, 1160
HinP1I	GCGC	2 cut(s)	191, 1160
HincII	GTYRAC	1 cut(s)	1350
HindII	GTYRAC	1 cut(s)	1350
HinfI	GANTC	5 cut(s)	6, 766, 1037, 1514, 1690
HpaII	CCGG	9 cut(s)	186, 275, 422, 543, 608, 1230, 1259, 1466, 1635
HphI	GGTGA	3 cut(s)	88, 464, 523
Hpy166II	GTNNAC	5 cut(s)	205, 856, 1235, 1350, 1713
Hpy188III	TCNNGA	8 cut(s)	186, 755, 950, 1253, 1580, 1655, 1770, 1788
Hpy8I	GTNNAC	5 cut(s)	205, 856, 1235, 1350, 1713
Hpy99I	CGWCG	1 cut(s)	488
HpyAV	CCTTC	5 cut(s)	174, 490, 560, 1256, 1776
HpyCH4III	ACNGT	5 cut(s)	387, 747, 1033, 1354, 1663
HpyCH4IV	ACGT	1 cut(s)	1017
HpyCH4V	TGCA	9 cut(s)	331, 821, 842, 1151, 1304, 1400, 1552, 1696, 1778
HpyF10VI	GCNNNNNNNGC	6 cut(s)	323, 337, 349, 358, 675, 1157
HpyF3I	CTNAG	7 cut(s)	10, 435, 555, 738, 781, 1026, 1682
HpySE526I	ACGT	1 cut(s)	1017
Hsp92I	GRCGYC	1 cut(s)	1762
Hsp92II	CATG	7 cut(s)	451, 632, 709, 734, 1155, 1256, 1505
HspAI	GCGC	2 cut(s)	191, 1160
Kpn2I	TCCGGA	1 cut(s)	185
Ksp22I	TGATCA	1 cut(s)	574
Kzo9I	GATC	9 cut(s)	136, 318, 574, 1411, 1488, 1543, 1618, 1671, 1729
LmnI	GCTCC	4 cut(s)	188, 349, 394, 889
Lsp1109I	GCAGC	4 cut(s)	1321, 1384, 1412, 1536
LweI	GCATC	4 cut(s)	808, 853, 1306, 1577
MaeI	CTAG	3 cut(s)	666, 1533, 1646
MaeII	ACGT	1 cut(s)	1017
MaeIII	GTNAC	1 cut(s)	1781
MalI	GATC	9 cut(s)	138, 320, 576, 1413, 1490, 1545, 1620, 1673, 1731
MbiI	CCGCTC	1 cut(s)	361
MboI	GATC	9 cut(s)	136, 318, 574, 1411, 1488, 1543, 1618, 1671, 1729
MboII	GAAGA	7 cut(s)	37, 85, 115, 118, 526, 896, 1558
MhlI	GDGCHC	2 cut(s)	354, 894
MlyI	GAGTC	1 cut(s)	1523
MmeI	TCCRAC	1 cut(s)	201
Mph1103I	ATGCAT	1 cut(s)	823
MroI	TCCGGA	1 cut(s)	185
MroXI	GAANNNNTTC	1 cut(s)	1575
MseI	TTAA	3 cut(s)	312, 830, 1628
MslI	CAYNNNNRTG	1 cut(s)	861
MspI	CCGG	9 cut(s)	186, 275, 422, 543, 608, 1230, 1259, 1466, 1635
MspR9I	CCNGG	7 cut(s)	392, 543, 544, 912, 1213, 1259, 1635
Mva1269I	GAATGC	2 cut(s)	1381, 1552
MvaI	CCWGG	3 cut(s)	392, 912, 1213
MvnI	CGCG	5 cut(s)	337, 357, 951, 1160, 1623
MwoI	GCNNNNNNNGC	6 cut(s)	323, 337, 349, 358, 675, 1157
NciI	CCSGG	4 cut(s)	543, 544, 1259, 1635
NdeI	CATATG	1 cut(s)	345
NdeII	GATC	9 cut(s)	136, 318, 574, 1411, 1488, 1543, 1618, 1671, 1729
NheI	GCTAGC	1 cut(s)	665
NlaIII	CATG	7 cut(s)	451, 632, 709, 734, 1155, 1256, 1505
NlaIV	GGNNCC	6 cut(s)	225, 260, 273, 389, 396, 420
NruI	TCGCGA	1 cut(s)	951
NsiI	ATGCAT	1 cut(s)	823
NspI	RCATGY	1 cut(s)	632
NspV	TTCGAA	2 cut(s)	251, 1569
PagI	TCATGA	1 cut(s)	1252
PctI	GAATGC	2 cut(s)	1381, 1552
PdmI	GAANNNNTTC	1 cut(s)	1575
PfeI	GAWTC	4 cut(s)	6, 766, 1037, 1690
PflMI	CCANNNNNTGG	1 cut(s)	1296
PinAI	ACCGGT	1 cut(s)	1229
PkrI	GCNGC	9 cut(s)	339, 342, 363, 378, 399, 1336, 1399, 1402, 1551
PleI	GAGTC	1 cut(s)	1522
PpsI	GAGTC	1 cut(s)	1522
Psp124BI	GAGCTC	1 cut(s)	894
Psp6I	CCWGG	3 cut(s)	390, 910, 1211
PspFI	CCCAGC	2 cut(s)	372, 1011
PspGI	CCWGG	3 cut(s)	390, 910, 1211
PspN4I	GGNNCC	6 cut(s)	225, 260, 273, 389, 396, 420
PspPI	GGNCC	5 cut(s)	272, 499, 1127, 1203, 1632
PstI	CTGCAG	1 cut(s)	1402
RruI	TCGCGA	1 cut(s)	951
RsaI	GTAC	5 cut(s)	268, 413, 491, 553, 1157
RsaNI	GTAC	5 cut(s)	267, 412, 490, 552, 1156
RseI	CAYNNNNRTG	1 cut(s)	861
SacI	GAGCTC	1 cut(s)	894
SalI	GTCGAC	1 cut(s)	1348
SaqAI	TTAA	3 cut(s)	312, 830, 1628
SatI	GCNGC	9 cut(s)	338, 341, 362, 377, 398, 1335, 1398, 1401, 1550
Sau3AI	GATC	9 cut(s)	136, 318, 574, 1411, 1488, 1543, 1618, 1671, 1729
Sau96I	GGNCC	5 cut(s)	272, 499, 1127, 1203, 1632
SchI	GAGTC	1 cut(s)	1523
ScrFI	CCNGG	7 cut(s)	392, 543, 544, 912, 1213, 1259, 1635
SduI	GDGCHC	2 cut(s)	354, 894
SfaNI	GCATC	4 cut(s)	808, 853, 1306, 1577
SfcI	CTRYAG	2 cut(s)	1398, 1713
SfuI	TTCGAA	2 cut(s)	251, 1569
SinI	GGWCC	3 cut(s)	272, 499, 1127
SmaI	CCCGGG	1 cut(s)	544
SmiMI	CAYNNNNRTG	1 cut(s)	861
SsiI	CCGC	9 cut(s)	168, 281, 337, 340, 355, 361, 377, 398, 1357
SspMI	CTAG	3 cut(s)	666, 1533, 1646
SstI	GAGCTC	1 cut(s)	894
StyD4I	CCNGG	7 cut(s)	390, 541, 542, 910, 1211, 1257, 1633
TaaI	ACNGT	5 cut(s)	387, 747, 1033, 1354, 1663
TaiI	ACGT	1 cut(s)	1020
TaqI	TCGA	9 cut(s)	251, 285, 486, 594, 1035, 1349, 1569, 1674, 1769
TaqII	GACCGA	1 cut(s)	1702
TatI	WGTACW	1 cut(s)	489
TauI	GCSGC	5 cut(s)	340, 343, 364, 379, 400
TfiI	GAWTC	4 cut(s)	6, 766, 1037, 1690
Tru1I	TTAA	3 cut(s)	312, 830, 1628
Tru9I	TTAA	3 cut(s)	312, 830, 1628
TscAI	CASTG	5 cut(s)	589, 789, 863, 1174, 1712
TseI	GCWGC	4 cut(s)	1334, 1397, 1400, 1549
TspDTI	ATGAA	8 cut(s)	580, 741, 747, 824, 972, 1388, 1436, 1445
TspGWI	ACGGA	1 cut(s)	285
TspMI	CCCGGG	1 cut(s)	542
TspRI	CASTG	5 cut(s)	589, 789, 863, 1174, 1712
Van91I	CCANNNNNTGG	1 cut(s)	1296
VpaK11BI	GGWCC	3 cut(s)	272, 499, 1127
XagI	CCTNNNNNAGG	1 cut(s)	1770
XapI	RAATTY	2 cut(s)	38, 247
XceI	RCATGY	1 cut(s)	632
XmaI	CCCGGG	1 cut(s)	542
XmiI	GTMKAC	3 cut(s)	204, 1349, 1712
XmnI	GAANNNNTTC	1 cut(s)	1575
XspI	CTAG	3 cut(s)	666, 1533, 1646
Zsp2I	ATGCAT	1 cut(s)	823

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.