MD01G1157500.v1.1

Belongs to the histidine acid phosphatase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012909
Physical Location & Seq
Reverse (-)
26451725 .. 26455905
4181 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1157500.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGGGGAGAGCCATGGCGGCGCTTCTGCTGGTATTGTTATCGGTGTTCGTCCATTCGAATGCCGGAGAAGAAGGTTTCGATGTTCGCCAACATCTTTCTACTGCGTCCAGATACGGTGTCGTTAAAGACATTGCGGACAACTCATTTGTACCATCCAAGATACCAGATGGGTGTACTCCTATCCACCTAAATCTTGTGGCAAGGCATGGAACCCGTGCTCCTACCAAAAAACGTATCAAAGATATGGATATCCTGGCCTCTCGTCTGGAGGTGCTACTAAAAGAAGCAGAAGAACAGAACCTATCTTTGGAAAAACTTCCTGGTTGGTTTTTGGGTTGGAAATCTCCTTGGAAAGGAAAACTGACAGGTGGAGAATTAATTGTCCAAGGAGAGGATGAACTCTATGATTTTGGAATCAGGACACGAGAAAGGTTCCCAAATCTGTTTGATCAGGATTACCATCCAGATGTTTACACAATAAAGGCAACACAGGTCCCTCGGGCATCCGCCAGTGCTGTGGCATTTGGCATGGGAATGTTTAGTGGGAAAGGAAATCTTGGACCTGGGCGTCATCGAGCTTTTGCTGTGTCCAGTGAAAGTCGTGCGAGTGATATAAAGTTGAGGTTTCATGATTGTTGCCAAAACTACAAGGCATATAAGAAAAGTCAGGAGCCTTCTGTTGACAAGCTCAAGGAACCTATCTATGATGAAATTACTTCTTCATTAAGGAGGCGCTACAAGTTGAATTTTACAAAGAAGGATACAACTTCTCTTTGGTTTCTTTGCAAACAGGAAGCGTCCTTGTTTAATATAACTGGTCAAGCTTGTGCGCTGTTCACCCCTTCCGAGGTTTCTTTGCTGGAGTGGGCAGATGATTTGGAAGCCTTTATATTAAAGGGATATGGTAAATCATTAAACTATAGAATGGGAGTTCCCTTGCTTGAAGATGTATTGCTGTCCATGGAGCAGGCTATTACGGCTGAAGAAGAAAAACATGCTCCTGGGAGTTATGAAAAGGCAAGGCTAAGGTTCGCACATGCAGAAACTGTAGTTCCATTTTCATGCCTGCTTGGACTTTTTCTTGATGGAACTGAATTTGAACGGACACAGAAGGAACAACCCTTGCAACTCCCTCCAAAGCCTCCCCAGAAGAGAAAGTGGAGGGGCAATACTGTGGCACCTTTTGGCGGGAATAATATGCTGGTTCTGTACAGTTGTCCAGCCAACATTTCAAGCAAGTACTTTGTGCAAGTGCTGCACAATGAACACCCCATTCCAATGCCAGGCTGTGATGGTTCTGATTTCTGTCCACTCGATGTTTTCAAGGAAAGAATAGTTGCTCCTCATCTGAAGCACGACTATAACTCAGTGTGCAATGCAAAATTGGAGGAAAAGGAGGCGAAGCCAGCCAGTAAGTTATCTCAACTGTTCCGTTGGTTGTTCTCACTGGGGAACGGTGATAAAACCCTCGATGAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00010 ko00562 ko01100 map00010 map00562 map01100
Pfam Domains
Protein Families
His_Phos_2

Protein Analysis

494

Amino Acids

55.44

Weight (kDa)

8.19

Isoelectric Point (pI)

38.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_2 PF00328 62 - 421 3.5e-52 Histidine phosphatase superfamily (branch 2)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012909)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09870
fragaria_vesca FvH4_7g25060 FvH4_7g25060
malus_domestica MD01G1157500.v1.1
prunus_persica Prupe.2G255400_v2.0.a1 Prupe.2G255400_v2.0.a1
pyrus_communis pycom01g17420
rosa_chinensis RchiOBHm_Chr1g0370931
rosa_laevigata RLG00000026998
rosa_multiflora Rmu_sc0001018.1_g000003
rosa_roxburghii Rroxscaffold_4G00286120
rosa_rugosa Rorug01G0360200 Rorug01G0360200
rosa_samantha Rh1AG370300 Rh1BG333900 Rh1CG347200 Rh1DG365100
rosa_wichuraiana Rw1G032590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 567
AccB1I GGYRCC 1 cut(s) 1177
AciI CCGC 4 cut(s) 17, 134, 507, 1188
AcsI RAATTY 2 cut(s) 745, 1094
AcuI CTGAAG 2 cut(s) 1002, 1370
AcyI GRCGYC 1 cut(s) 568
AfaI GTAC 4 cut(s) 150, 175, 1211, 1241
AfiI CCNNNNNNNGG 6 cut(s) 307, 353, 391, 847, 1187, 1283
AgsI TTSAA 5 cut(s) 745, 944, 1100, 1233, 1324
AjnI CCWGG 5 cut(s) 252, 319, 562, 1000, 1282
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AloI GAACNNNNNNTCC 2 cut(s) 202, 234
AluBI AGCT 3 cut(s) 578, 688, 824
AluI AGCT 3 cut(s) 578, 688, 824
Alw21I GWGCWC 1 cut(s) 220
AlwNI CAGNNNCTG 1 cut(s) 1046
Ama87I CYCGRG 1 cut(s) 498
AoxI GGCC 1 cut(s) 255
ApeKI GCWGC 1 cut(s) 1255
ApoI RAATTY 2 cut(s) 745, 1094
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
AseI ATTAAT 1 cut(s) 377
Asp700I GAANNNNTTC 1 cut(s) 315
AspLEI GCGC 3 cut(s) 22, 735, 832
AspS9I GGNCC 2 cut(s) 493, 560
AsuHPI GGTGA 2 cut(s) 829, 1469
AsuII TTCGAA 1 cut(s) 56
AvaI CYCGRG 1 cut(s) 498
AvaII GGWCC 2 cut(s) 493, 560
BanI GGYRCC 1 cut(s) 1177
BauI CACGAG 1 cut(s) 423
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 1 cut(s) 1242
BccI CCATC 5 cut(s) 160, 161, 468, 1079, 1286
BceAI ACGGC 1 cut(s) 993
BciT130I CCWGG 5 cut(s) 254, 321, 564, 1002, 1284
BciVI GTATCC 1 cut(s) 754
BclI TGATCA 1 cut(s) 448
BfmI CTRYAG 2 cut(s) 919, 1047
BfoI RGCGCY 2 cut(s) 23, 736
BfuI GTATCC 1 cut(s) 754
BglI GCCNNNNNGGC 1 cut(s) 17
BisI GCNGC 2 cut(s) 18, 1256
BlsI GCNGC 2 cut(s) 19, 1257
BmcAI AGTACT 1 cut(s) 1241
Bme1390I CCNGG 5 cut(s) 254, 321, 564, 1002, 1284
Bme18I GGWCC 2 cut(s) 493, 560
BmeT110I CYCGRG 1 cut(s) 498
BmgT120I GGNCC 2 cut(s) 493, 560
BmiI GGNNCC 6 cut(s) 211, 434, 495, 672, 696, 1179
BmrFI CCNGG 5 cut(s) 254, 321, 564, 1002, 1284
BmrI ACTGGG 1 cut(s) 1457
BmsI GCATC 1 cut(s) 512
BmuI ACTGGG 1 cut(s) 1457
BpmI CTGGAG 2 cut(s) 287, 881
Bpu10I CCTNAGC 1 cut(s) 1025
Bpu14I TTCGAA 1 cut(s) 56
BpuEI CTTGAG 1 cut(s) 674
BsaBI GATNNNNATC 1 cut(s) 459
BsaHI GRCGYC 1 cut(s) 568
BsaJI CCNNGG 8 cut(s) 12, 347, 385, 497, 563, 846, 960, 1001
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 6 cut(s) 307, 353, 391, 847, 1187, 1283
Bse1I ACTGG 5 cut(s) 510, 591, 820, 1410, 1452
Bse3DI GCAATG 2 cut(s) 129, 1381
Bse8I GATNNNNATC 1 cut(s) 459
BseBI CCWGG 5 cut(s) 254, 321, 564, 1002, 1284
BseDI CCNNGG 8 cut(s) 12, 347, 385, 497, 563, 846, 960, 1001
BseGI GGATG 4 cut(s) 152, 400, 460, 503
BseJI GATNNNNATC 1 cut(s) 459
BseLI CCNNNNNNNGG 6 cut(s) 307, 353, 391, 847, 1187, 1283
BseMI GCAATG 2 cut(s) 129, 1381
BseMII CTCAG 1 cut(s) 1380
BseNI ACTGG 5 cut(s) 510, 591, 820, 1410, 1452
BseRI GAGGAG 1 cut(s) 1332
BseXI GCAGC 1 cut(s) 1242
BsgI GTGCAG 1 cut(s) 1241
BshFI GGCC 1 cut(s) 257
BshNI GGYRCC 1 cut(s) 1177
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 498
BsiSI CCGG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 6 cut(s) 307, 353, 391, 847, 1187, 1283
BsmFI GGGAC 1 cut(s) 479
BsmI GAATGC 1 cut(s) 64
BsnI GGCC 1 cut(s) 257
BsoBI CYCGRG 1 cut(s) 498
Bsp119I TTCGAA 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 220
Bsp1407I TGTACA 1 cut(s) 1209
Bsp143I GATC 1 cut(s) 448
Bsp19I CCATGG 2 cut(s) 12, 960
BspACI CCGC 4 cut(s) 17, 134, 507, 1188
BspANI GGCC 1 cut(s) 257
BspCNI CTCAG 1 cut(s) 1379
BspHI TCATGA 1 cut(s) 628
BspLI GGNNCC 6 cut(s) 211, 434, 495, 672, 696, 1179
BspT104I TTCGAA 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 1177
BsrDI GCAATG 2 cut(s) 129, 1381
BsrGI TGTACA 1 cut(s) 1209
BsrI ACTGG 5 cut(s) 510, 591, 820, 1410, 1452
BssECI CCNNGG 8 cut(s) 12, 347, 385, 497, 563, 846, 960, 1001
BssMI GATC 1 cut(s) 448
BssNI GRCGYC 1 cut(s) 568
BssSI CACGAG 1 cut(s) 423
BssT1I CCWWGG 4 cut(s) 12, 347, 385, 960
Bst2BI CACGAG 1 cut(s) 423
Bst2UI CCWGG 5 cut(s) 254, 321, 564, 1002, 1284
Bst4CI ACNGT 6 cut(s) 116, 1048, 1174, 1214, 1428, 1457
Bst6I CTCTTC 1 cut(s) 1145
BstACI GRCGYC 1 cut(s) 568
BstAPI GCANNNNNTGC 1 cut(s) 1255
BstAUI TGTACA 1 cut(s) 1209
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 3 cut(s) 969, 1067, 1407
BstDEI CTNAG 2 cut(s) 1025, 1366
BstDSI CCRYGG 2 cut(s) 12, 960
BstENI CCTNNNNNAGG 1 cut(s) 351
BstF5I GGATG 4 cut(s) 152, 400, 460, 503
BstH2I RGCGCY 2 cut(s) 23, 736
BstHHI GCGC 3 cut(s) 22, 735, 832
BstKTI GATC 1 cut(s) 451
BstMBI GATC 1 cut(s) 448
BstMWI GCNNNNNNNGC 4 cut(s) 17, 977, 1255, 1406
BstNI CCWGG 5 cut(s) 254, 321, 564, 1002, 1284
BstNSI RCATGY 2 cut(s) 998, 1040
BstSCI CCNGG 5 cut(s) 252, 319, 562, 1000, 1282
BstSFI CTRYAG 2 cut(s) 919, 1047
BstV1I GCAGC 1 cut(s) 1242
BstXI CCANNNNNNTGG 1 cut(s) 517
BsuI GTATCC 1 cut(s) 754
BsuRI GGCC 1 cut(s) 257
BtgI CCRYGG 2 cut(s) 12, 960
BtsCI GGATG 4 cut(s) 152, 400, 460, 503
BtsIMutI CAGTG 4 cut(s) 517, 598, 1374, 1445
Cac8I GCNNGC 3 cut(s) 969, 1067, 1407
CaiI CAGNNNCTG 1 cut(s) 1046
CciI TCATGA 1 cut(s) 628
CfoI GCGC 3 cut(s) 22, 735, 832
Cfr13I GGNCC 2 cut(s) 493, 560
CseI GACGC 3 cut(s) 93, 557, 786
Csp6I GTAC 4 cut(s) 149, 174, 1210, 1240
CviAII CATG 8 cut(s) 13, 206, 529, 629, 961, 995, 1037, 1062
CviQI GTAC 4 cut(s) 149, 174, 1210, 1240
DdeI CTNAG 2 cut(s) 1025, 1366
DpnI GATC 1 cut(s) 450
DpnII GATC 1 cut(s) 448
DrdI GACNNNNNNGTC 1 cut(s) 567
DseDI GACNNNNNNGTC 1 cut(s) 567
Eam1104I CTCTTC 1 cut(s) 1145
EarI CTCTTC 1 cut(s) 1145
EciI GGCGGA 1 cut(s) 496
Eco130I CCWWGG 4 cut(s) 12, 347, 385, 960
Eco32I GATATC 1 cut(s) 250
Eco47I GGWCC 2 cut(s) 493, 560
Eco57I CTGAAG 2 cut(s) 1002, 1370
Eco88I CYCGRG 1 cut(s) 498
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 493
EcoRII CCWGG 5 cut(s) 252, 319, 562, 1000, 1282
EcoRV GATATC 1 cut(s) 250
EcoT14I CCWWGG 4 cut(s) 12, 347, 385, 960
ErhI CCWWGG 4 cut(s) 12, 347, 385, 960
FaeI CATG 8 cut(s) 16, 209, 532, 632, 964, 998, 1040, 1065
FalI AAGNNNNNCTT 2 cut(s) 540, 572
FaqI GGGAC 1 cut(s) 479
FatI CATG 8 cut(s) 12, 205, 528, 628, 960, 994, 1036, 1061
FauI CCCGC 1 cut(s) 1181
FbaI TGATCA 1 cut(s) 448
Fnu4HI GCNGC 2 cut(s) 18, 1256
FokI GGATG 4 cut(s) 139, 407, 447, 490
Fsp4HI GCNGC 2 cut(s) 18, 1256
GlaI GCGC 3 cut(s) 21, 734, 831
GluI GCNGC 2 cut(s) 18, 1256
GsuI CTGGAG 2 cut(s) 287, 881
HaeII RGCGCY 2 cut(s) 23, 736
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 63
HgaI GACGC 3 cut(s) 93, 557, 786
HhaI GCGC 3 cut(s) 22, 735, 832
Hin1I GRCGYC 1 cut(s) 568
Hin1II CATG 8 cut(s) 16, 209, 532, 632, 964, 998, 1040, 1065
Hin6I GCGC 3 cut(s) 20, 733, 830
HinP1I GCGC 3 cut(s) 20, 733, 830
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HindIII AAGCTT 1 cut(s) 822
HinfI GANTC 1 cut(s) 414
HpaII CCGG 1 cut(s) 63
HphI GGTGA 2 cut(s) 829, 1469
Hpy166II GTNNAC 5 cut(s) 174, 472, 682, 837, 1310
Hpy188I TCNGA 3 cut(s) 847, 1300, 1350
Hpy188III TCNNGA 8 cut(s) 108, 266, 418, 452, 464, 629, 668, 1082
Hpy8I GTNNAC 5 cut(s) 174, 472, 682, 837, 1310
HpyAV CCTTC 5 cut(s) 65, 684, 751, 852, 1105
HpyCH4III ACNGT 6 cut(s) 116, 1048, 1174, 1214, 1428, 1457
HpyCH4IV ACGT 1 cut(s) 232
HpyCH4V TGCA 7 cut(s) 786, 1040, 1126, 1249, 1258, 1374, 1379
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 977, 1255, 1406
HpyF3I CTNAG 2 cut(s) 1025, 1366
HpySE526I ACGT 1 cut(s) 232
Hsp92I GRCGYC 1 cut(s) 568
Hsp92II CATG 8 cut(s) 16, 209, 532, 632, 964, 998, 1040, 1065
HspAI GCGC 3 cut(s) 20, 733, 830
Ksp22I TGATCA 1 cut(s) 448
Kzo9I GATC 1 cut(s) 448
LmnI GCTCC 5 cut(s) 223, 670, 964, 1003, 1345
Lsp1109I GCAGC 1 cut(s) 1242
LweI GCATC 1 cut(s) 512
MaeII ACGT 1 cut(s) 232
MalI GATC 1 cut(s) 450
MboI GATC 1 cut(s) 448
MboII GAAGA 7 cut(s) 80, 302, 711, 956, 995, 998, 1162
MhlI GDGCHC 1 cut(s) 220
MluCI AATT 7 cut(s) 374, 378, 711, 745, 1094, 1382, 1475
MmeI TCCRAC 1 cut(s) 317
MroXI GAANNNNTTC 1 cut(s) 315
MseI TTAA 6 cut(s) 123, 377, 725, 807, 893, 914
MslI CAYNNNNRTG 5 cut(s) 57, 465, 533, 1060, 1277
MspI CCGG 1 cut(s) 63
MspR9I CCNGG 5 cut(s) 254, 321, 564, 1002, 1284
Mva1269I GAATGC 1 cut(s) 64
MvaI CCWGG 5 cut(s) 254, 321, 564, 1002, 1284
MwoI GCNNNNNNNGC 4 cut(s) 17, 977, 1255, 1406
NcoI CCATGG 2 cut(s) 12, 960
NdeII GATC 1 cut(s) 448
NlaIII CATG 8 cut(s) 16, 209, 532, 632, 964, 998, 1040, 1065
NlaIV GGNNCC 6 cut(s) 211, 434, 495, 672, 696, 1179
NspI RCATGY 2 cut(s) 998, 1040
NspV TTCGAA 1 cut(s) 56
PagI TCATGA 1 cut(s) 628
PctI GAATGC 1 cut(s) 64
PdmI GAANNNNTTC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 414
PkrI GCNGC 2 cut(s) 19, 1257
PpuMI RGGWCCY 1 cut(s) 493
PshBI ATTAAT 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 493
Psp6I CCWGG 5 cut(s) 252, 319, 562, 1000, 1282
PspGI CCWGG 5 cut(s) 252, 319, 562, 1000, 1282
PspN4I GGNNCC 6 cut(s) 211, 434, 495, 672, 696, 1179
PspPI GGNCC 2 cut(s) 493, 560
PspPPI RGGWCCY 1 cut(s) 493
PstNI CAGNNNCTG 1 cut(s) 1046
RsaI GTAC 4 cut(s) 150, 175, 1211, 1241
RsaNI GTAC 4 cut(s) 149, 174, 1210, 1240
RseI CAYNNNNRTG 5 cut(s) 57, 465, 533, 1060, 1277
SaqAI TTAA 6 cut(s) 123, 377, 725, 807, 893, 914
SatI GCNGC 2 cut(s) 18, 1256
Sau3AI GATC 1 cut(s) 448
Sau96I GGNCC 2 cut(s) 493, 560
ScaI AGTACT 1 cut(s) 1241
ScrFI CCNGG 5 cut(s) 254, 321, 564, 1002, 1284
SduI GDGCHC 1 cut(s) 220
SfaNI GCATC 1 cut(s) 512
SfcI CTRYAG 2 cut(s) 919, 1047
SfuI TTCGAA 1 cut(s) 56
SinI GGWCC 2 cut(s) 493, 560
SmiMI CAYNNNNRTG 5 cut(s) 57, 465, 533, 1060, 1277
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 7 cut(s) 374, 378, 711, 745, 1094, 1382, 1475
SsiI CCGC 4 cut(s) 17, 134, 507, 1188
StyD4I CCNGG 5 cut(s) 252, 319, 562, 1000, 1282
StyI CCWWGG 4 cut(s) 12, 347, 385, 960
TaaI ACNGT 6 cut(s) 116, 1048, 1174, 1214, 1428, 1457
TaiI ACGT 1 cut(s) 235
TaqI TCGA 5 cut(s) 56, 78, 574, 1314, 1470
TasI AATT 7 cut(s) 374, 378, 711, 745, 1094, 1382, 1475
TatI WGTACW 3 cut(s) 173, 1209, 1239
TauI GCSGC 1 cut(s) 20
TfiI GAWTC 1 cut(s) 414
Tru1I TTAA 6 cut(s) 123, 377, 725, 807, 893, 914
Tru9I TTAA 6 cut(s) 123, 377, 725, 807, 893, 914
TscAI CASTG 4 cut(s) 517, 598, 1374, 1452
TseI GCWGC 1 cut(s) 1255
TspDTI ATGAA 7 cut(s) 411, 617, 711, 723, 1026, 1050, 1278
TspGWI ACGGA 2 cut(s) 1117, 1421
TspRI CASTG 4 cut(s) 517, 598, 1374, 1452
VpaK11BI GGWCC 2 cut(s) 493, 560
VspI ATTAAT 1 cut(s) 377
XagI CCTNNNNNAGG 1 cut(s) 351
XapI RAATTY 2 cut(s) 745, 1094
XceI RCATGY 2 cut(s) 998, 1040
XmnI GAANNNNTTC 1 cut(s) 315
ZrmI AGTACT 1 cut(s) 1241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.