Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013716

Physical Location & Seq

Genomic Coordinates Forward (+)

26577585 .. 26581267

Gene Structure 3683 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1159500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGATGAGGCAGGAAAACCACGGCGACCAGGAGGCCGACCTACATGCATCCCAAGATGACATGGAATCTCTCGTCCTTGACGAGCCTCCCTCATCCAACGGCCAATCCTCGCCCTCCGCGTCCACCTCCGTGGACCGCCACTCCGCCACAGCAACAACAGTCACATCCTCGCAAGGCTTCAATTCCATCCTTGAACCCCCGTCTTACGCCGACGCCATCTTCACCTCGTTCGATTCCTCCTCATCCAACGGCCACGAAAGCCCTAGGCCCTCATCTTATCAATCGGAGCCTCCGACAAGATCCGAATTTCTCAAGATATGGGTCTCGGACCCTCAGAAGGAGCTCGAGCTCAACAATTCGCTTGTTCCCGGCGGGACCTCGTACTGCACGTACCTGATCACCACTCGGACCAATATGCCCGAGTACGGCGGGCCCGGATCCGAATTCACCCTACGGAGGCGGTTCAAGGACGTGGTGACGCTGTCGGACCGGCTATCCGAATCGTTCCGCGGGTTCTTCATCCCGATCCGACCCGATAAGAGTGTGGTGGAGAGCCAGGTGATGCAAATCGAGCAGTTCGTGGAGCTGCGGAGGGCGGCACTGGCGAAGTACCTGAACAAGCTCGCGGTGCATCCGACGATCAAGAAGAGCGAGGAGCTGAGGCTGTTTTTGGTGGTGAAGGGGAAGGTACCGCTAACAAGGAGCATTGACGTGGCGTCGCGGATGCTCGATGGAGCAGTGAGGTTACCAAGGCAGCTGACGGGGGAGGTGGCTGTCGTGACAGATGTGAGTGAGGCTGCGCAGCCGGCCAAGGGCGGCAGGGACTTGCTGAGGATCTTCAGGGAGCTGAAGCAGGCAGTGGTGAACGATTGGGGCGGAGTGAAGCCAATGGCGGTTGAGGACGATAAGGAATTTTTGGAGAGGAAGGAGAAGGTGGCGGAGTTTGAGCAGCAGCTTAGCAATTTGTCTCAGCAGGCTGAATCACTAGTAAAAGCTCAGCAGGACATGGGAGAGACTCTGGGGGAATTGGGGTTGGCATTTGTGAAGCTGACCAAGCTTGAGACGGAGGGAGCCATGTTCGAATCTCAAACAATACGAGCCAATGACATGAAAAATGTGGCTACTGCTTCTGTTAAAGCTAGCCGATTGTACCGGGAGTTAAATGCGCAAACGGTCAAACATTTGGACAAACTCCATGAATATCTTGGAGTAATGCTGGCAGTCAATAGTGCATTTTCAGACAGATCAAGTGCTTTACTGACGGTTCAGACTCTTTCGTCGGAATTAGTTTCATTGCAGTCCAGGATTGAGAAGCTTGAAGCTGCAGCATCCAAAGTATTTGGTGGAGACAGGTCCAGGATGCGGAAAATAGAGGAGCTTAAAGAAACTTTAAAGATTAGTGAGGATGCTAAATCTTGTGCAATCAGAGAGTATGAACGCATCAAGGAAAATAACAGGAGTGAGCTCGAAAGGCTTGACAGAGAGAGGCACGAGGACTTCATGGGCATGCTGAGAGGATTTGTTCTTAACCAGGCTGGATACGCGGAAAAGATGGCGAATACTTGGGAGAAGCTTGCTGAAGAAACAAGCGGGTATGCGAATGATGCCAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005794 GO:0005802 GO:0005829 GO:0006807 GO:0006810 GO:0006897 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009791 GO:0009987 GO:0010008 GO:0010467 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019538 GO:0019898 GO:0030904 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032585 GO:0032588 GO:0032991 GO:0035091 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048856 GO:0051179 GO:0051234 GO:0051604 GO:0071704 GO:0071840 GO:0090351 GO:0097708 GO:0098588 GO:0098657 GO:0098791 GO:0098796 GO:0098805 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

PX Vps5

Protein Analysis

538

Amino Acids

59.94

Weight (kDa)

5.45

Isoelectric Point (pI)

48.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PX	PF00787	146 - 225	6e-14	PX domain
Vps5	PF09325	298 - 527	2.6e-18	Vps5 C terminal like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013716)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g25340 FvH4_7g25340 FvH4_7g25340
malus_domestica	MD01G1159500.v1.1 MD07G1228000.v1.1
prunus_persica	Prupe.2G257500_v2.0.a1
pyrus_communis	pycom01g17580 pycom07g20640
rosa_chinensis	RchiOBHm_Chr1g0371251
rosa_laevigata	RLG00000026976
rosa_roxburghii	Rroxscaffold_4G00285840
rosa_rugosa	Rorug01G0362400 Rorug01G0362400
rosa_samantha	Rh1AG372500 Rh1BG336000 Rh1CG349400 Rh1DG367100
rosa_wichuraiana	Rw1G032760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1276
Acc16I	TGCGCA	2 cut(s)	801, 1167
Acc65I	GGTACC	1 cut(s)	688
AccB1I	GGYRCC	1 cut(s)	688
AccII	CGCG	5 cut(s)	119, 510, 626, 721, 1544
AclWI	GGATC	5 cut(s)	294, 432, 445, 520, 842
AcoI	YGGCCR	3 cut(s)	100, 250, 807
AcsI	RAATTY	3 cut(s)	305, 443, 911
AcuI	CTGAAG	3 cut(s)	823, 869, 1599
AcyI	GRCGYC	2 cut(s)	213, 716
AfaI	GTAC	6 cut(s)	383, 392, 425, 611, 690, 1151
AfiI	CCNNNNNNNGG	5 cut(s)	337, 425, 456, 812, 1362
AgsI	TTSAA	4 cut(s)	181, 194, 466, 1319
AhdI	GACNNNNNGTC	1 cut(s)	715
AhlI	ACTAGT	1 cut(s)	985
AjiI	CACGTC	2 cut(s)	472, 712
AjnI	CCWGG	5 cut(s)	27, 555, 1301, 1355, 1530
AleI	CACNNNNGTG	1 cut(s)	128
Alw21I	GWGCWC	3 cut(s)	345, 351, 1467
Alw26I	GTCTC	5 cut(s)	328, 972, 1007, 1055, 1341
AlwI	GGATC	5 cut(s)	294, 432, 445, 520, 842
Ama87I	CYCGRG	2 cut(s)	344, 419
AoxI	GGCC	6 cut(s)	33, 100, 250, 266, 431, 807
ApaI	GGGCCC	1 cut(s)	435
ApeKI	GCWGC	8 cut(s)	586, 754, 797, 802, 949, 952, 1322, 1325
ApoI	RAATTY	3 cut(s)	305, 443, 911
Asp718I	GGTACC	1 cut(s)	688
AspA2I	CCTAGG	1 cut(s)	263
AspLEI	GCGC	2 cut(s)	802, 1168
AspS9I	GGNCC	9 cut(s)	133, 267, 328, 375, 408, 431, 432, 487, 1353
AsuC2I	CCSGG	3 cut(s)	369, 435, 1154
AsuHPI	GGTGA	7 cut(s)	214, 391, 439, 487, 571, 688, 874
AsuII	TTCGAA	1 cut(s)	1080
AsuNHI	GCTAGC	1 cut(s)	1139
AvaI	CYCGRG	2 cut(s)	344, 419
AvaII	GGWCC	6 cut(s)	133, 328, 375, 408, 487, 1353
AvrII	CCTAGG	1 cut(s)	263
BaeGI	GKGCMC	1 cut(s)	435
BamHI	GGATCC	1 cut(s)	437
BanI	GGYRCC	1 cut(s)	688
BanII	GRGCYC	4 cut(s)	345, 351, 435, 1467
BauI	CACGAG	1 cut(s)	1490
Bbv12I	GWGCWC	3 cut(s)	345, 351, 1467
BbvCI	CCTCAGC	2 cut(s)	659, 830
BbvI	GCAGC	8 cut(s)	573, 766, 784, 814, 961, 964, 1309, 1337
BccI	CCATC	4 cut(s)	194, 224, 725, 1546
BceAI	ACGGC	4 cut(s)	37, 115, 265, 442
BcgI	CGANNNNNNTGC	2 cut(s)	26, 60
BciT130I	CCWGG	5 cut(s)	29, 557, 1303, 1357, 1532
BciVI	GTATCC	1 cut(s)	1532
BclI	TGATCA	1 cut(s)	396
BcnI	CCSGG	3 cut(s)	369, 435, 1154
BcoDI	GTCTC	5 cut(s)	328, 972, 1007, 1055, 1341
BcuI	ACTAGT	1 cut(s)	985
BfaI	CTAG	3 cut(s)	264, 986, 1140
BfmI	CTRYAG	1 cut(s)	1323
BfuI	GTATCC	1 cut(s)	1532
BlnI	CCTAGG	1 cut(s)	263
BlpI	GCTNAGC	2 cut(s)	956, 996
Bme1390I	CCNGG	8 cut(s)	29, 369, 435, 557, 1154, 1303, 1357, 1532
Bme18I	GGWCC	6 cut(s)	133, 328, 375, 408, 487, 1353
BmeRI	GACNNNNNGTC	1 cut(s)	715
BmeT110I	CYCGRG	2 cut(s)	344, 419
BmgBI	CACGTC	2 cut(s)	472, 712
BmgT120I	GGNCC	9 cut(s)	133, 267, 328, 375, 408, 431, 432, 487, 1353
BmiI	GGNNCC	7 cut(s)	288, 330, 376, 433, 439, 690, 1072
BmrFI	CCNGG	8 cut(s)	29, 369, 435, 557, 1154, 1303, 1357, 1532
BmsI	GCATC	9 cut(s)	56, 552, 640, 714, 1337, 1350, 1396, 1449, 1594
BmtI	GCTAGC	1 cut(s)	1143
BplI	GAGNNNNNCTC	2 cut(s)	74, 106
Bpu10I	CCTNAGC	2 cut(s)	659, 830
Bpu1102I	GCTNAGC	2 cut(s)	956, 996
Bpu14I	TTCGAA	1 cut(s)	1080
BpuEI	CTTGAG	2 cut(s)	296, 1079
BpuMI	CCSGG	3 cut(s)	369, 435, 1154
BsaAI	YACGTR	1 cut(s)	390
BsaBI	GATNNNNATC	1 cut(s)	566
BsaHI	GRCGYC	2 cut(s)	213, 716
BsaI	GGTCTC	1 cut(s)	328
BsaJI	CCNNGG	6 cut(s)	19, 129, 263, 508, 749, 810
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	5 cut(s)	337, 425, 456, 812, 1362
Bse118I	RCCGGY	2 cut(s)	489, 805
Bse1I	ACTGG	1 cut(s)	606
Bse3DI	GCAATG	1 cut(s)	1292
Bse8I	GATNNNNATC	1 cut(s)	566
BseBI	CCWGG	5 cut(s)	29, 557, 1303, 1357, 1532
BseDI	CCNNGG	6 cut(s)	19, 129, 263, 508, 749, 810
BseJI	GATNNNNATC	1 cut(s)	566
BseLI	CCNNNNNNNGG	5 cut(s)	337, 425, 456, 812, 1362
BseMI	GCAATG	1 cut(s)	1292
BseMII	CTCAG	6 cut(s)	347, 650, 821, 983, 1010, 1502
BseNI	ACTGG	1 cut(s)	606
BseRI	GAGGAG	3 cut(s)	229, 668, 1388
BseSI	GKGCMC	1 cut(s)	435
BseXI	GCAGC	8 cut(s)	573, 766, 784, 814, 961, 964, 1309, 1337
BsgI	GTGCAG	1 cut(s)	370
Bsh1236I	CGCG	5 cut(s)	119, 510, 626, 721, 1544
BshFI	GGCC	6 cut(s)	35, 102, 252, 268, 433, 809
BshNI	GGYRCC	1 cut(s)	688
BsiHKAI	GWGCWC	3 cut(s)	345, 351, 1467
BsiHKCI	CYCGRG	2 cut(s)	344, 419
BsiSI	CCGG	5 cut(s)	369, 435, 490, 806, 1153
BslFI	GGGAC	2 cut(s)	388, 836
BslI	CCNNNNNNNGG	5 cut(s)	337, 425, 456, 812, 1362
BsmAI	GTCTC	5 cut(s)	328, 972, 1007, 1055, 1341
BsmBI	CGTCTC	1 cut(s)	1055
BsmFI	GGGAC	2 cut(s)	388, 836
BsnI	GGCC	6 cut(s)	35, 102, 252, 268, 433, 809
Bso31I	GGTCTC	1 cut(s)	328
BsoBI	CYCGRG	2 cut(s)	344, 419
Bsp119I	TTCGAA	1 cut(s)	1080
Bsp120I	GGGCCC	1 cut(s)	431
Bsp1286I	GDGCHC	4 cut(s)	345, 351, 435, 1467
Bsp143I	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
Bsp1720I	GCTNAGC	2 cut(s)	956, 996
BspANI	GGCC	6 cut(s)	35, 102, 252, 268, 433, 809
BspCNI	CTCAG	6 cut(s)	346, 651, 822, 982, 1009, 1503
BspFNI	CGCG	5 cut(s)	119, 510, 626, 721, 1544
BspLI	GGNNCC	7 cut(s)	288, 330, 376, 433, 439, 690, 1072
BspMAI	CTGCAG	1 cut(s)	1327
BspOI	GCTAGC	1 cut(s)	1143
BspPI	GGATC	5 cut(s)	294, 432, 445, 520, 842
BspQI	GCTCTTC	1 cut(s)	641
BspT104I	TTCGAA	1 cut(s)	1080
BspT107I	GGYRCC	1 cut(s)	688
BspTNI	GGTCTC	1 cut(s)	328
BsrDI	GCAATG	1 cut(s)	1292
BsrFI	RCCGGY	2 cut(s)	489, 805
BsrI	ACTGG	1 cut(s)	606
BssAI	RCCGGY	2 cut(s)	489, 805
BssECI	CCNNGG	6 cut(s)	19, 129, 263, 508, 749, 810
BssMI	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
BssNI	GRCGYC	2 cut(s)	213, 716
BssSI	CACGAG	1 cut(s)	1490
BssT1I	CCWWGG	3 cut(s)	263, 749, 810
Bst2BI	CACGAG	1 cut(s)	1490
Bst2UI	CCWGG	5 cut(s)	29, 557, 1303, 1357, 1532
Bst4CI	ACNGT	3 cut(s)	160, 1174, 1264
Bst6I	CTCTTC	1 cut(s)	641
BstACI	GRCGYC	2 cut(s)	213, 716
BstBAI	YACGTR	1 cut(s)	390
BstBI	TTCGAA	1 cut(s)	1080
BstDEI	CTNAG	7 cut(s)	333, 659, 830, 956, 969, 996, 1511
BstDSI	CCRYGG	3 cut(s)	19, 129, 508
BstEII	GGTNACC	1 cut(s)	744
BstFNI	CGCG	5 cut(s)	119, 510, 626, 721, 1544
BstHHI	GCGC	2 cut(s)	802, 1168
BstKTI	GATC	7 cut(s)	302, 399, 440, 528, 642, 837, 1247
BstMAI	GTCTC	5 cut(s)	328, 972, 1007, 1055, 1341
BstMBI	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
BstMWI	GCNNNNNNNGC	9 cut(s)	258, 571, 602, 628, 806, 1054, 1471, 1541, 1604
BstNI	CCWGG	5 cut(s)	29, 557, 1303, 1357, 1532
BstNSI	RCATGY	2 cut(s)	47, 1510
BstPI	GGTNACC	1 cut(s)	744
BstSCI	CCNGG	8 cut(s)	27, 367, 433, 555, 1152, 1301, 1355, 1530
BstSFI	CTRYAG	1 cut(s)	1323
BstSLI	GKGCMC	1 cut(s)	435
BstUI	CGCG	5 cut(s)	119, 510, 626, 721, 1544
BstV1I	GCAGC	8 cut(s)	573, 766, 784, 814, 961, 964, 1309, 1337
BstX2I	RGATCY	3 cut(s)	299, 437, 834
BstXI	CCANNNNNNTGG	1 cut(s)	130
BstYI	RGATCY	3 cut(s)	299, 437, 834
BsuI	GTATCC	1 cut(s)	1532
BsuRI	GGCC	6 cut(s)	35, 102, 252, 268, 433, 809
BtgI	CCRYGG	3 cut(s)	19, 129, 508
BtrI	CACGTC	2 cut(s)	472, 712
BtsI	GCAGTG	2 cut(s)	744, 864
BtsIMutI	CAGTG	3 cut(s)	599, 744, 864
CfoI	GCGC	2 cut(s)	802, 1168
Cfr10I	RCCGGY	2 cut(s)	489, 805
Cfr13I	GGNCC	9 cut(s)	133, 267, 328, 375, 408, 431, 432, 487, 1353
Cfr42I	CCGCGG	1 cut(s)	511
CpoI	CGGWCCG	1 cut(s)	487
CseI	GACGC	4 cut(s)	108, 221, 487, 705
Csp6I	GTAC	6 cut(s)	382, 391, 424, 610, 689, 1150
CspI	CGGWCCG	1 cut(s)	487
CviAII	CATG	8 cut(s)	44, 61, 1006, 1075, 1108, 1196, 1501, 1507
CviQI	GTAC	6 cut(s)	382, 391, 424, 610, 689, 1150
DdeI	CTNAG	7 cut(s)	333, 659, 830, 956, 969, 996, 1511
DpnI	GATC	7 cut(s)	301, 398, 439, 527, 641, 836, 1246
DpnII	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
DraI	TTTAAA	1 cut(s)	1392
DrdI	GACNNNNNNGTC	1 cut(s)	1276
DriI	GACNNNNNGTC	1 cut(s)	715
DseDI	GACNNNNNNGTC	1 cut(s)	1276
EaeI	YGGCCR	3 cut(s)	100, 250, 807
Eam1104I	CTCTTC	1 cut(s)	641
Eam1105I	GACNNNNNGTC	1 cut(s)	715
EarI	CTCTTC	1 cut(s)	641
EciI	GGCGGA	3 cut(s)	133, 891, 953
Ecl136II	GAGCTC	3 cut(s)	343, 349, 1465
Eco130I	CCWWGG	3 cut(s)	263, 749, 810
Eco24I	GRGCYC	4 cut(s)	345, 351, 435, 1467
Eco31I	GGTCTC	1 cut(s)	328
Eco47I	GGWCC	6 cut(s)	133, 328, 375, 408, 487, 1353
Eco53kI	GAGCTC	3 cut(s)	343, 349, 1465
Eco57I	CTGAAG	3 cut(s)	823, 869, 1599
Eco88I	CYCGRG	2 cut(s)	344, 419
Eco91I	GGTNACC	1 cut(s)	744
EcoICRI	GAGCTC	3 cut(s)	343, 349, 1465
EcoO109I	RGGNCCY	2 cut(s)	267, 375
EcoO65I	GGTNACC	1 cut(s)	744
EcoRI	GAATTC	1 cut(s)	443
EcoRII	CCWGG	5 cut(s)	27, 555, 1301, 1355, 1530
EcoT14I	CCWWGG	3 cut(s)	263, 749, 810
EcoT22I	ATGCAT	1 cut(s)	49
EcoT38I	GRGCYC	4 cut(s)	345, 351, 435, 1467
ErhI	CCWWGG	3 cut(s)	263, 749, 810
Esp3I	CGTCTC	1 cut(s)	1055
FaeI	CATG	8 cut(s)	47, 64, 1009, 1078, 1111, 1199, 1504, 1510
FaqI	GGGAC	2 cut(s)	388, 836
FatI	CATG	8 cut(s)	43, 60, 1005, 1074, 1107, 1195, 1500, 1506
FauI	CCCGC	4 cut(s)	365, 422, 503, 1583
FbaI	TGATCA	1 cut(s)	396
FriOI	GRGCYC	4 cut(s)	345, 351, 435, 1467
FspBI	CTAG	3 cut(s)	264, 986, 1140
FspI	TGCGCA	2 cut(s)	801, 1167
GlaI	GCGC	2 cut(s)	801, 1167
HaeIII	GGCC	6 cut(s)	35, 102, 252, 268, 433, 809
HapII	CCGG	5 cut(s)	369, 435, 490, 806, 1153
HgaI	GACGC	4 cut(s)	108, 221, 487, 705
HhaI	GCGC	2 cut(s)	802, 1168
Hin1I	GRCGYC	2 cut(s)	213, 716
Hin1II	CATG	8 cut(s)	47, 64, 1009, 1078, 1111, 1199, 1504, 1510
Hin6I	GCGC	2 cut(s)	800, 1166
HinP1I	GCGC	2 cut(s)	800, 1166
HindIII	AAGCTT	3 cut(s)	1055, 1313, 1571
HinfI	GANTC	7 cut(s)	65, 233, 500, 980, 1015, 1082, 1270
HpaII	CCGG	5 cut(s)	369, 435, 490, 806, 1153
HphI	GGTGA	7 cut(s)	214, 391, 439, 487, 571, 688, 874
Hpy166II	GTNNAC	3 cut(s)	123, 133, 865
Hpy188III	TCNNGA	4 cut(s)	313, 523, 643, 778
Hpy8I	GTNNAC	3 cut(s)	123, 133, 865
Hpy99I	CGWCG	4 cut(s)	215, 640, 721, 1282
HpyAV	CCTTC	5 cut(s)	331, 673, 679, 919, 925
HpyCH4III	ACNGT	3 cut(s)	160, 1174, 1264
HpyCH4IV	ACGT	3 cut(s)	389, 471, 711
HpyCH4V	TGCA	8 cut(s)	47, 387, 565, 631, 1232, 1297, 1325, 1421
HpyF10VI	GCNNNNNNNGC	9 cut(s)	258, 571, 602, 628, 806, 1054, 1471, 1541, 1604
HpyF3I	CTNAG	7 cut(s)	333, 659, 830, 956, 969, 996, 1511
HpySE526I	ACGT	3 cut(s)	389, 471, 711
Hsp92I	GRCGYC	2 cut(s)	213, 716
Hsp92II	CATG	8 cut(s)	47, 64, 1009, 1078, 1111, 1199, 1504, 1510
HspAI	GCGC	2 cut(s)	800, 1166
KpnI	GGTACC	1 cut(s)	692
KroI	GCCGGC	1 cut(s)	805
KroNI	GCCGGC	1 cut(s)	807
Ksp22I	TGATCA	1 cut(s)	396
KspI	CCGCGG	1 cut(s)	511
Kzo9I	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
LguI	GCTCTTC	1 cut(s)	641
LmnI	GCTCC	9 cut(s)	286, 340, 583, 655, 702, 734, 844, 1070, 1375
Lsp1109I	GCAGC	8 cut(s)	573, 766, 784, 814, 961, 964, 1309, 1337
LweI	GCATC	9 cut(s)	56, 552, 640, 714, 1337, 1350, 1396, 1449, 1594
MaeI	CTAG	3 cut(s)	264, 986, 1140
MaeII	ACGT	3 cut(s)	389, 471, 711
MaeIII	GTNAC	4 cut(s)	160, 475, 744, 778
MalI	GATC	7 cut(s)	301, 398, 439, 527, 641, 836, 1246
MboI	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
MboII	GAAGA	5 cut(s)	211, 508, 658, 829, 1592
MflI	RGATCY	3 cut(s)	299, 437, 834
MhlI	GDGCHC	4 cut(s)	345, 351, 435, 1467
MluCI	AATT	8 cut(s)	181, 305, 355, 443, 911, 961, 1025, 1283
MlyI	GAGTC	2 cut(s)	1009, 1264
MmeI	TCCRAC	7 cut(s)	120, 270, 317, 465, 553, 659, 1260
Mph1103I	ATGCAT	1 cut(s)	49
MroNI	GCCGGC	1 cut(s)	805
MseI	TTAA	5 cut(s)	1134, 1160, 1380, 1391, 1527
MslI	CAYNNNNRTG	3 cut(s)	128, 710, 1505
MspA1I	CMGCKG	3 cut(s)	510, 757, 1611
MspI	CCGG	5 cut(s)	369, 435, 490, 806, 1153
MspR9I	CCNGG	8 cut(s)	29, 369, 435, 557, 1154, 1303, 1357, 1532
MvaI	CCWGG	5 cut(s)	29, 557, 1303, 1357, 1532
MvnI	CGCG	5 cut(s)	119, 510, 626, 721, 1544
MwoI	GCNNNNNNNGC	9 cut(s)	258, 571, 602, 628, 806, 1054, 1471, 1541, 1604
NaeI	GCCGGC	1 cut(s)	807
NciI	CCSGG	3 cut(s)	369, 435, 1154
NdeII	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
NgoMIV	GCCGGC	1 cut(s)	805
NheI	GCTAGC	1 cut(s)	1139
NlaIII	CATG	8 cut(s)	47, 64, 1009, 1078, 1111, 1199, 1504, 1510
NlaIV	GGNNCC	7 cut(s)	288, 330, 376, 433, 439, 690, 1072
NmuCI	GTSAC	3 cut(s)	160, 475, 778
NsbI	TGCGCA	2 cut(s)	801, 1167
NsiI	ATGCAT	1 cut(s)	49
NspI	RCATGY	2 cut(s)	47, 1510
NspV	TTCGAA	1 cut(s)	1080
OliI	CACNNNNGTG	1 cut(s)	128
PaeI	GCATGC	1 cut(s)	1510
PaeR7I	CTCGAG	1 cut(s)	344
PciSI	GCTCTTC	1 cut(s)	641
PcsI	WCGNNNNNNNCGW	5 cut(s)	78, 116, 290, 386, 576
PdiI	GCCGGC	1 cut(s)	807
PfeI	GAWTC	5 cut(s)	65, 233, 500, 980, 1082
PflFI	GACNNNGTC	1 cut(s)	481
PfoI	TCCNGGA	2 cut(s)	1301, 1355
PleI	GAGTC	2 cut(s)	1009, 1264
PpsI	GAGTC	2 cut(s)	1009, 1264
Ppu21I	YACGTR	1 cut(s)	390
PpuMI	RGGWCCY	1 cut(s)	375
Psp124BI	GAGCTC	3 cut(s)	345, 351, 1467
Psp5II	RGGWCCY	1 cut(s)	375
Psp6I	CCWGG	5 cut(s)	27, 555, 1301, 1355, 1530
PspEI	GGTNACC	1 cut(s)	744
PspGI	CCWGG	5 cut(s)	27, 555, 1301, 1355, 1530
PspN4I	GGNNCC	7 cut(s)	288, 330, 376, 433, 439, 690, 1072
PspOMI	GGGCCC	1 cut(s)	431
PspPI	GGNCC	9 cut(s)	133, 267, 328, 375, 408, 431, 432, 487, 1353
PspPPI	RGGWCCY	1 cut(s)	375
PspXI	VCTCGAGB	1 cut(s)	344
PstI	CTGCAG	1 cut(s)	1327
PsuI	RGATCY	3 cut(s)	299, 437, 834
PsyI	GACNNNGTC	1 cut(s)	481
PvuII	CAGCTG	2 cut(s)	757, 1611
RsaI	GTAC	6 cut(s)	383, 392, 425, 611, 690, 1151
RsaNI	GTAC	6 cut(s)	382, 391, 424, 610, 689, 1150
RseI	CAYNNNNRTG	3 cut(s)	128, 710, 1505
Rsr2I	CGGWCCG	1 cut(s)	487
RsrII	CGGWCCG	1 cut(s)	487
SacI	GAGCTC	3 cut(s)	345, 351, 1467
SacII	CCGCGG	1 cut(s)	511
SapI	GCTCTTC	1 cut(s)	641
SaqAI	TTAA	5 cut(s)	1134, 1160, 1380, 1391, 1527
Sau3AI	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
Sau96I	GGNCC	9 cut(s)	133, 267, 328, 375, 408, 431, 432, 487, 1353
SchI	GAGTC	2 cut(s)	1009, 1264
ScrFI	CCNGG	8 cut(s)	29, 369, 435, 557, 1154, 1303, 1357, 1532
SduI	GDGCHC	4 cut(s)	345, 351, 435, 1467
SfaNI	GCATC	9 cut(s)	56, 552, 640, 714, 1337, 1350, 1396, 1449, 1594
SfcI	CTRYAG	1 cut(s)	1323
Sfr274I	CTCGAG	1 cut(s)	344
Sfr303I	CCGCGG	1 cut(s)	511
SfuI	TTCGAA	1 cut(s)	1080
SgrBI	CCGCGG	1 cut(s)	511
SinI	GGWCC	6 cut(s)	133, 328, 375, 408, 487, 1353
SlaI	CTCGAG	1 cut(s)	344
SmiMI	CAYNNNNRTG	3 cut(s)	128, 710, 1505
SmlI	CTYRAG	3 cut(s)	311, 344, 1058
SmoI	CTYRAG	3 cut(s)	311, 344, 1058
SpeI	ACTAGT	1 cut(s)	985
SphI	GCATGC	1 cut(s)	1510
Sse9I	AATT	8 cut(s)	181, 305, 355, 443, 911, 961, 1025, 1283
SspMI	CTAG	3 cut(s)	264, 986, 1140
SstI	GAGCTC	3 cut(s)	345, 351, 1467
StyD4I	CCNGG	8 cut(s)	27, 367, 433, 555, 1152, 1301, 1355, 1530
StyI	CCWWGG	3 cut(s)	263, 749, 810
TaaI	ACNGT	3 cut(s)	160, 1174, 1264
TaiI	ACGT	3 cut(s)	392, 474, 714
TaqI	TCGA	6 cut(s)	231, 345, 570, 729, 1080, 1467
TasI	AATT	8 cut(s)	181, 305, 355, 443, 911, 961, 1025, 1283
TauI	GCSGC	2 cut(s)	599, 819
TfiI	GAWTC	5 cut(s)	65, 233, 500, 980, 1082
Tru1I	TTAA	5 cut(s)	1134, 1160, 1380, 1391, 1527
Tru9I	TTAA	5 cut(s)	1134, 1160, 1380, 1391, 1527
TscAI	CASTG	3 cut(s)	606, 744, 864
TseFI	GTSAC	3 cut(s)	160, 475, 778
TseI	GCWGC	8 cut(s)	586, 754, 797, 802, 949, 952, 1322, 1325
Tsp45I	GTSAC	3 cut(s)	160, 475, 778
TspDTI	ATGAA	6 cut(s)	508, 1124, 1212, 1281, 1449, 1489
TspGWI	ACGGA	3 cut(s)	118, 469, 1079
TspRI	CASTG	3 cut(s)	606, 744, 864
Tth111I	GACNNNGTC	1 cut(s)	481
VpaK11BI	GGWCC	6 cut(s)	133, 328, 375, 408, 487, 1353
XapI	RAATTY	3 cut(s)	305, 443, 911
XceI	RCATGY	2 cut(s)	47, 1510
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1202
XhoI	CTCGAG	1 cut(s)	344
XmaJI	CCTAGG	1 cut(s)	263
XspI	CTAG	3 cut(s)	264, 986, 1140
Zsp2I	ATGCAT	1 cut(s)	49

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.