Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0013716

Physical Location & Seq

Genomic Coordinates Forward (+)

30346072 .. 30349533

Gene Structure 3462 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1228000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1614 bp

ATGATGAGGCAGGAAAACGACGGCGACCAGGAGGCCGACCTACACGCATCCCAAGACGACATGGAATCTCTCGTCCTCGACGAGCCACCCTCATCCAACGGCCAATCCTCTCCCTCCGCGTCCACCTCCGTAGACCGCCACTCCGCCACCGCGACAACGGCCACCTCCTCGCAAGGCTTCAATTCCATCCTTGAACCCCCGTCTTACGCTGACGCCATCTTCACCTCGTTCGATTCCTCCTCATCCAACGGCCACGACTGCCCTAAGCCCTCCTCTTATCAATCGGAGCCTCCGACAAGATCCGAATTTCTCAAGATATGGGTCACGGACCCTCAGAAAGAGCTCGAGCTCTCCAATTCGCTTGTTCCCGGCGGGACCTCGTACCACACGTACCTGATCACCACCCGGACCAACATGCCCGAGTACGGCGGGCCCGGATCCGAGTTCACCGTCCGGAGGCGGTTCAAGGACGTGGTGACGCTGGCGGACCGGCTATCCGAATCGTTTCGCGGGTTCTTCATCCCGATCCGACCCGATAAGAGCGTGGTGGATAGTCAGGTGATGCAAATCGAGCAATTCGTGGAGCTGCGGAGGGCGGCATTGGCGAAGTACCTGAACAAGCTCGCGGTGCATCCGACGATCAAGAAGAGCGAGGAGCTGAGGCTGTTTTTGGTCATGAAGGGGAAGCTGCCGCTGACGAGGAGCGTGGACATGGCTTCGCGCATGCTCGACGGAGCAGTGAGGCTGCCGAGGCAGTTAACGGGCGAGGTGACGGCTGTGACGGATGTGAATGAGGCTGCGCAGCCGGCCAAGGGCGGCAGGGACTTGCTGAGGATCTTCAGGGAGCTGAAGCAGTCGGTGGTGAACGATTGGGGAGGCGTGAAGCCGATGGCGGTTGAGGATGATAAGGAGTTTTTGGCGAGGAAGGAGAAGGTGGCGGAATTTGAGCAGCAGCTTAGCAATTTGTCTCAGCAGGCTGAATCACTAGTAAAAGCTCAGCAGGACATGGGAGAGACTATGGGAGAATTGGGGTTGGCGTTTGTGAAGCTGACCAAGCTTGAGACGGAGGGAGCCATGTTTGAGTCTCAAACATTACGAGCGAATGACATGAAAAATGTGGCTACTGCTTCTGTTAAAGCTAGTCGATTGTACCGGGAGTTAAATTCGCAAACGGTCAAACATTTGGACAAACTTCATGAATATCTTGGAGTAGTGCTAGCAGTCAATAGTGCATTTTCAGACAGATCAAGTGCTTTACTGACAGTTCAGACTCTTTCATCAGAATTAGTTTCGTTGCAGTCCAGGATTGAGAAGCTTGAAGCTGCAGCATCCAAAATATTTGGTGGAGACAGGTCCAGGATGCGGAAAATAGAGGAGCTTAAAGAAACTTTAAAAATTAGCGAGGATGCTAAATCTTGTGCAGTCAGAGAGTATGAACGAATCAAGGAAAATAACAGGAGTGAGCTCGAAAGGCTCGACAGAGAGAGGCACGAGGACTTCATGGGCATGCTACGAGGGTTTGTTCTTAACCAGGCAGGATATGCGGAAAAGATGGCGAATGCTTGGGAGAAGCTTGCGGAGGAAACAAGCGGGTATGCTAAAGATGGCAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005794 GO:0005802 GO:0005829 GO:0006807 GO:0006810 GO:0006897 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009791 GO:0009987 GO:0010008 GO:0010467 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019538 GO:0019898 GO:0030904 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032585 GO:0032588 GO:0032991 GO:0035091 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048856 GO:0051179 GO:0051234 GO:0051604 GO:0071704 GO:0071840 GO:0090351 GO:0097708 GO:0098588 GO:0098657 GO:0098791 GO:0098796 GO:0098805 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

PX Vps5

Protein Analysis

538

Amino Acids

59.87

Weight (kDa)

5.51

Isoelectric Point (pI)

47.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PX	PF00787	146 - 224	1.1e-14	PX domain
Vps5	PF09325	298 - 527	1.8e-18	Vps5 C terminal like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013716)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g25340 FvH4_7g25340 FvH4_7g25340
malus_domestica	MD01G1159500.v1.1 MD07G1228000.v1.1
prunus_persica	Prupe.2G257500_v2.0.a1
pyrus_communis	pycom01g17580 pycom07g20640
rosa_chinensis	RchiOBHm_Chr1g0371251
rosa_laevigata	RLG00000026976
rosa_roxburghii	Rroxscaffold_4G00285840
rosa_rugosa	Rorug01G0362400 Rorug01G0362400
rosa_samantha	Rh1AG372500 Rh1BG336000 Rh1CG349400 Rh1DG367100
rosa_wichuraiana	Rw1G032760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	801
AccI	GTMKAC	1 cut(s)	132
AccII	CGCG	5 cut(s)	119, 152, 510, 626, 721
AccIII	TCCGGA	1 cut(s)	453
AclWI	GGATC	5 cut(s)	294, 432, 445, 520, 842
AcoI	YGGCCR	4 cut(s)	100, 159, 250, 807
AcsI	RAATTY	3 cut(s)	305, 941, 1162
AcuI	CTGAAG	2 cut(s)	823, 869
AcyI	GRCGYC	1 cut(s)	213
AfaI	GTAC	5 cut(s)	383, 392, 425, 611, 1151
AfiI	CCNNNNNNNGG	4 cut(s)	425, 456, 812, 1362
AflIII	ACRYGT	1 cut(s)	387
AgsI	TTSAA	4 cut(s)	181, 194, 466, 1319
AhlI	ACTAGT	1 cut(s)	985
AjiI	CACGTC	1 cut(s)	472
AjnI	CCWGG	4 cut(s)	27, 1301, 1355, 1530
Alw21I	GWGCWC	3 cut(s)	345, 351, 1467
Alw26I	GTCTC	5 cut(s)	972, 1007, 1055, 1089, 1341
AlwI	GGATC	5 cut(s)	294, 432, 445, 520, 842
Ama87I	CYCGRG	2 cut(s)	344, 419
Aor13HI	TCCGGA	1 cut(s)	453
AoxI	GGCC	6 cut(s)	33, 100, 159, 250, 431, 807
ApaI	GGGCCC	1 cut(s)	435
ApoI	RAATTY	3 cut(s)	305, 941, 1162
Asp700I	GAANNNNTTC	1 cut(s)	504
AspLEI	GCGC	2 cut(s)	723, 802
AspS9I	GGNCC	7 cut(s)	328, 375, 408, 431, 432, 487, 1353
AsuC2I	CCSGG	4 cut(s)	369, 406, 435, 1154
AsuHPI	GGTGA	7 cut(s)	214, 391, 439, 487, 571, 781, 874
AsuNHI	GCTAGC	1 cut(s)	1216
AvaI	CYCGRG	2 cut(s)	344, 419
AvaII	GGWCC	5 cut(s)	328, 375, 408, 487, 1353
BaeGI	GKGCMC	1 cut(s)	435
BamHI	GGATCC	1 cut(s)	437
BanII	GRGCYC	4 cut(s)	345, 351, 435, 1467
BauI	CACGAG	1 cut(s)	1490
Bbv12I	GWGCWC	3 cut(s)	345, 351, 1467
BbvCI	CCTCAGC	2 cut(s)	659, 830
BbvI	GCAGC	9 cut(s)	573, 675, 732, 784, 814, 961, 964, 1309, 1337
BccI	CCATC	5 cut(s)	194, 224, 883, 1546, 1598
BceAI	ACGGC	6 cut(s)	37, 115, 174, 265, 442, 789
BciT130I	CCWGG	4 cut(s)	29, 1303, 1357, 1532
BclI	TGATCA	1 cut(s)	396
BcnI	CCSGG	4 cut(s)	369, 406, 435, 1154
BcoDI	GTCTC	5 cut(s)	972, 1007, 1055, 1089, 1341
BcuI	ACTAGT	1 cut(s)	985
BfaI	CTAG	3 cut(s)	986, 1140, 1217
BfmI	CTRYAG	1 cut(s)	1323
BlpI	GCTNAGC	2 cut(s)	956, 996
Bme1390I	CCNGG	8 cut(s)	29, 369, 406, 435, 1154, 1303, 1357, 1532
Bme18I	GGWCC	5 cut(s)	328, 375, 408, 487, 1353
BmeT110I	CYCGRG	2 cut(s)	344, 419
BmgBI	CACGTC	1 cut(s)	472
BmgT120I	GGNCC	7 cut(s)	328, 375, 408, 431, 432, 487, 1353
BmiI	GGNNCC	6 cut(s)	288, 330, 376, 433, 439, 1072
BmrFI	CCNGG	8 cut(s)	29, 369, 406, 435, 1154, 1303, 1357, 1532
BmsI	GCATC	6 cut(s)	56, 552, 640, 1337, 1350, 1396
BmtI	GCTAGC	1 cut(s)	1220
BplI	GAGNNNNNCTC	2 cut(s)	74, 106
Bpu10I	CCTNAGC	3 cut(s)	264, 659, 830
Bpu1102I	GCTNAGC	2 cut(s)	956, 996
BpuEI	CTTGAG	2 cut(s)	296, 1079
BpuMI	CCSGG	4 cut(s)	369, 406, 435, 1154
BsaAI	YACGTR	1 cut(s)	390
BsaBI	GATNNNNATC	1 cut(s)	566
BsaHI	GRCGYC	1 cut(s)	213
BsaJI	CCNNGG	2 cut(s)	749, 810
BsaWI	WCCGGW	1 cut(s)	453
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	4 cut(s)	425, 456, 812, 1362
Bse118I	RCCGGY	2 cut(s)	489, 805
Bse8I	GATNNNNATC	1 cut(s)	566
BseAI	TCCGGA	1 cut(s)	453
BseBI	CCWGG	4 cut(s)	29, 1303, 1357, 1532
BseDI	CCNNGG	2 cut(s)	749, 810
BseJI	GATNNNNATC	1 cut(s)	566
BseLI	CCNNNNNNNGG	4 cut(s)	425, 456, 812, 1362
BseMII	CTCAG	5 cut(s)	347, 650, 821, 983, 1010
BseRI	GAGGAG	6 cut(s)	157, 229, 262, 668, 715, 1388
BseSI	GKGCMC	1 cut(s)	435
BseXI	GCAGC	9 cut(s)	573, 675, 732, 784, 814, 961, 964, 1309, 1337
BsgI	GTGCAG	1 cut(s)	1440
Bsh1236I	CGCG	5 cut(s)	119, 152, 510, 626, 721
BshFI	GGCC	6 cut(s)	35, 102, 161, 252, 433, 809
BsiHKAI	GWGCWC	3 cut(s)	345, 351, 1467
BsiHKCI	CYCGRG	2 cut(s)	344, 419
BsiSI	CCGG	7 cut(s)	369, 406, 435, 454, 490, 806, 1153
BslFI	GGGAC	2 cut(s)	388, 836
BslI	CCNNNNNNNGG	4 cut(s)	425, 456, 812, 1362
BsmAI	GTCTC	5 cut(s)	972, 1007, 1055, 1089, 1341
BsmBI	CGTCTC	1 cut(s)	1055
BsmFI	GGGAC	2 cut(s)	388, 836
BsmI	GAATGC	1 cut(s)	1564
BsnI	GGCC	6 cut(s)	35, 102, 161, 252, 433, 809
BsoBI	CYCGRG	2 cut(s)	344, 419
Bsp120I	GGGCCC	1 cut(s)	431
Bsp1286I	GDGCHC	4 cut(s)	345, 351, 435, 1467
Bsp13I	TCCGGA	1 cut(s)	453
Bsp143I	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
Bsp1720I	GCTNAGC	2 cut(s)	956, 996
BspANI	GGCC	6 cut(s)	35, 102, 161, 252, 433, 809
BspCNI	CTCAG	5 cut(s)	346, 651, 822, 982, 1009
BspEI	TCCGGA	1 cut(s)	453
BspFNI	CGCG	5 cut(s)	119, 152, 510, 626, 721
BspHI	TCATGA	2 cut(s)	675, 1195
BspLI	GGNNCC	6 cut(s)	288, 330, 376, 433, 439, 1072
BspMAI	CTGCAG	1 cut(s)	1327
BspOI	GCTAGC	1 cut(s)	1220
BspPI	GGATC	5 cut(s)	294, 432, 445, 520, 842
BspQI	GCTCTTC	1 cut(s)	641
BsrFI	RCCGGY	2 cut(s)	489, 805
BssAI	RCCGGY	2 cut(s)	489, 805
BssECI	CCNNGG	2 cut(s)	749, 810
BssMI	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
BssNI	GRCGYC	1 cut(s)	213
BssSI	CACGAG	1 cut(s)	1490
BssT1I	CCWWGG	1 cut(s)	810
Bst2BI	CACGAG	1 cut(s)	1490
Bst2UI	CCWGG	4 cut(s)	29, 1303, 1357, 1532
Bst4CI	ACNGT	3 cut(s)	451, 1174, 1264
Bst6I	CTCTTC	1 cut(s)	641
BstACI	GRCGYC	1 cut(s)	213
BstAPI	GCANNNNNTGC	1 cut(s)	1541
BstBAI	YACGTR	1 cut(s)	390
BstC8I	GCNNGC	9 cut(s)	431, 483, 624, 725, 807, 975, 1218, 1508, 1575
BstDEI	CTNAG	7 cut(s)	264, 333, 659, 830, 956, 969, 996
BstFNI	CGCG	5 cut(s)	119, 152, 510, 626, 721
BstHHI	GCGC	2 cut(s)	723, 802
BstKTI	GATC	7 cut(s)	302, 399, 440, 528, 642, 837, 1247
BstMAI	GTCTC	5 cut(s)	972, 1007, 1055, 1089, 1341
BstMBI	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
BstNI	CCWGG	4 cut(s)	29, 1303, 1357, 1532
BstNSI	RCATGY	3 cut(s)	418, 727, 1510
BstSCI	CCNGG	8 cut(s)	27, 367, 404, 433, 1152, 1301, 1355, 1530
BstSFI	CTRYAG	1 cut(s)	1323
BstSLI	GKGCMC	1 cut(s)	435
BstUI	CGCG	5 cut(s)	119, 152, 510, 626, 721
BstV1I	GCAGC	9 cut(s)	573, 675, 732, 784, 814, 961, 964, 1309, 1337
BstX2I	RGATCY	3 cut(s)	299, 437, 834
BstYI	RGATCY	3 cut(s)	299, 437, 834
BsuRI	GGCC	6 cut(s)	35, 102, 161, 252, 433, 809
BtrI	CACGTC	1 cut(s)	472
BtsI	GCAGTG	1 cut(s)	744
BtsIMutI	CAGTG	1 cut(s)	744
Cac8I	GCNNGC	9 cut(s)	431, 483, 624, 725, 807, 975, 1218, 1508, 1575
CciI	TCATGA	2 cut(s)	675, 1195
CfoI	GCGC	2 cut(s)	723, 802
Cfr10I	RCCGGY	2 cut(s)	489, 805
Cfr13I	GGNCC	7 cut(s)	328, 375, 408, 431, 432, 487, 1353
CpoI	CGGWCCG	1 cut(s)	487
CseI	GACGC	3 cut(s)	108, 221, 487
Csp6I	GTAC	5 cut(s)	382, 391, 424, 610, 1150
CspI	CGGWCCG	1 cut(s)	487
CviQI	GTAC	5 cut(s)	382, 391, 424, 610, 1150
DdeI	CTNAG	7 cut(s)	264, 333, 659, 830, 956, 969, 996
DpnI	GATC	7 cut(s)	301, 398, 439, 527, 641, 836, 1246
DpnII	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
DraI	TTTAAA	1 cut(s)	1392
EaeI	YGGCCR	4 cut(s)	100, 159, 250, 807
Eam1104I	CTCTTC	1 cut(s)	641
EarI	CTCTTC	1 cut(s)	641
EciI	GGCGGA	3 cut(s)	133, 500, 953
Ecl136II	GAGCTC	3 cut(s)	343, 349, 1465
Eco130I	CCWWGG	1 cut(s)	810
Eco24I	GRGCYC	4 cut(s)	345, 351, 435, 1467
Eco47I	GGWCC	5 cut(s)	328, 375, 408, 487, 1353
Eco53kI	GAGCTC	3 cut(s)	343, 349, 1465
Eco57I	CTGAAG	2 cut(s)	823, 869
Eco88I	CYCGRG	2 cut(s)	344, 419
EcoICRI	GAGCTC	3 cut(s)	343, 349, 1465
EcoO109I	RGGNCCY	1 cut(s)	375
EcoRII	CCWGG	4 cut(s)	27, 1301, 1355, 1530
EcoT14I	CCWWGG	1 cut(s)	810
EcoT38I	GRGCYC	4 cut(s)	345, 351, 435, 1467
ErhI	CCWWGG	1 cut(s)	810
Esp3I	CGTCTC	1 cut(s)	1055
FaqI	GGGAC	2 cut(s)	388, 836
FauI	CCCGC	4 cut(s)	365, 422, 503, 1583
FbaI	TGATCA	1 cut(s)	396
FblI	GTMKAC	1 cut(s)	132
FriOI	GRGCYC	4 cut(s)	345, 351, 435, 1467
FspBI	CTAG	3 cut(s)	986, 1140, 1217
FspI	TGCGCA	1 cut(s)	801
GlaI	GCGC	2 cut(s)	722, 801
HaeIII	GGCC	6 cut(s)	35, 102, 161, 252, 433, 809
HapII	CCGG	7 cut(s)	369, 406, 435, 454, 490, 806, 1153
HgaI	GACGC	3 cut(s)	108, 221, 487
HhaI	GCGC	2 cut(s)	723, 802
Hin1I	GRCGYC	1 cut(s)	213
Hin6I	GCGC	2 cut(s)	721, 800
HinP1I	GCGC	2 cut(s)	721, 800
HincII	GTYRAC	1 cut(s)	759
HindII	GTYRAC	1 cut(s)	759
HindIII	AAGCTT	3 cut(s)	1055, 1313, 1571
HinfI	GANTC	7 cut(s)	65, 233, 500, 980, 1082, 1270, 1440
HpaI	GTTAAC	1 cut(s)	759
HpaII	CCGG	7 cut(s)	369, 406, 435, 454, 490, 806, 1153
HphI	GGTGA	7 cut(s)	214, 391, 439, 487, 571, 781, 874
Hpy166II	GTNNAC	6 cut(s)	123, 133, 447, 709, 759, 865
Hpy188III	TCNNGA	6 cut(s)	313, 454, 523, 643, 676, 1196
Hpy8I	GTNNAC	6 cut(s)	123, 133, 447, 709, 759, 865
Hpy99I	CGWCG	4 cut(s)	23, 83, 640, 734
HpyAV	CCTTC	3 cut(s)	673, 919, 925
HpyCH4III	ACNGT	3 cut(s)	451, 1174, 1264
HpyCH4IV	ACGT	2 cut(s)	389, 471
HpyCH4V	TGCA	6 cut(s)	565, 631, 1232, 1297, 1325, 1421
HpyF3I	CTNAG	7 cut(s)	264, 333, 659, 830, 956, 969, 996
HpySE526I	ACGT	2 cut(s)	389, 471
Hsp92I	GRCGYC	1 cut(s)	213
HspAI	GCGC	2 cut(s)	721, 800
Kpn2I	TCCGGA	1 cut(s)	453
KroI	GCCGGC	1 cut(s)	805
KroNI	GCCGGC	1 cut(s)	807
Ksp22I	TGATCA	1 cut(s)	396
KspAI	GTTAAC	1 cut(s)	759
Kzo9I	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
LguI	GCTCTTC	1 cut(s)	641
LmnI	GCTCC	8 cut(s)	286, 583, 655, 702, 734, 844, 1070, 1375
Lsp1109I	GCAGC	9 cut(s)	573, 675, 732, 784, 814, 961, 964, 1309, 1337
LweI	GCATC	6 cut(s)	56, 552, 640, 1337, 1350, 1396
MaeI	CTAG	3 cut(s)	986, 1140, 1217
MaeII	ACGT	2 cut(s)	389, 471
MaeIII	GTNAC	4 cut(s)	322, 475, 769, 778
MalI	GATC	7 cut(s)	301, 398, 439, 527, 641, 836, 1246
MboI	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
MboII	GAAGA	4 cut(s)	211, 508, 658, 829
MflI	RGATCY	3 cut(s)	299, 437, 834
MhlI	GDGCHC	4 cut(s)	345, 351, 435, 1467
MlyI	GAGTC	2 cut(s)	1091, 1264
MmeI	TCCRAC	5 cut(s)	120, 270, 317, 553, 659
MroI	TCCGGA	1 cut(s)	453
MroNI	GCCGGC	1 cut(s)	805
MroXI	GAANNNNTTC	1 cut(s)	504
MseI	TTAA	6 cut(s)	758, 1134, 1160, 1380, 1391, 1527
MslI	CAYNNNNRTG	1 cut(s)	1505
MspA1I	CMGCKG	2 cut(s)	694, 1611
MspI	CCGG	7 cut(s)	369, 406, 435, 454, 490, 806, 1153
MspR9I	CCNGG	8 cut(s)	29, 369, 406, 435, 1154, 1303, 1357, 1532
Mva1269I	GAATGC	1 cut(s)	1564
MvaI	CCWGG	4 cut(s)	29, 1303, 1357, 1532
MvnI	CGCG	5 cut(s)	119, 152, 510, 626, 721
NaeI	GCCGGC	1 cut(s)	807
NciI	CCSGG	4 cut(s)	369, 406, 435, 1154
NdeII	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
NgoMIV	GCCGGC	1 cut(s)	805
NheI	GCTAGC	1 cut(s)	1216
NlaIV	GGNNCC	6 cut(s)	288, 330, 376, 433, 439, 1072
NmeAIII	GCCGAG	1 cut(s)	774
NmuCI	GTSAC	4 cut(s)	322, 475, 769, 778
NsbI	TGCGCA	1 cut(s)	801
NspI	RCATGY	3 cut(s)	418, 727, 1510
PaeI	GCATGC	2 cut(s)	727, 1510
PaeR7I	CTCGAG	1 cut(s)	344
PagI	TCATGA	2 cut(s)	675, 1195
PciSI	GCTCTTC	1 cut(s)	641
PcsI	WCGNNNNNNNCGW	5 cut(s)	78, 290, 386, 576, 1473
PctI	GAATGC	1 cut(s)	1564
PdiI	GCCGGC	1 cut(s)	807
PdmI	GAANNNNTTC	1 cut(s)	504
PfeI	GAWTC	5 cut(s)	65, 233, 500, 980, 1440
PfoI	TCCNGGA	2 cut(s)	1301, 1355
PleI	GAGTC	2 cut(s)	1090, 1264
PpsI	GAGTC	2 cut(s)	1090, 1264
Ppu21I	YACGTR	1 cut(s)	390
PpuMI	RGGWCCY	1 cut(s)	375
Psp124BI	GAGCTC	3 cut(s)	345, 351, 1467
Psp5II	RGGWCCY	1 cut(s)	375
Psp6I	CCWGG	4 cut(s)	27, 1301, 1355, 1530
PspGI	CCWGG	4 cut(s)	27, 1301, 1355, 1530
PspN4I	GGNNCC	6 cut(s)	288, 330, 376, 433, 439, 1072
PspOMI	GGGCCC	1 cut(s)	431
PspPI	GGNCC	7 cut(s)	328, 375, 408, 431, 432, 487, 1353
PspPPI	RGGWCCY	1 cut(s)	375
PspXI	VCTCGAGB	1 cut(s)	344
PstI	CTGCAG	1 cut(s)	1327
PsuI	RGATCY	3 cut(s)	299, 437, 834
PvuII	CAGCTG	1 cut(s)	1611
RsaI	GTAC	5 cut(s)	383, 392, 425, 611, 1151
RsaNI	GTAC	5 cut(s)	382, 391, 424, 610, 1150
RseI	CAYNNNNRTG	1 cut(s)	1505
Rsr2I	CGGWCCG	1 cut(s)	487
RsrII	CGGWCCG	1 cut(s)	487
SacI	GAGCTC	3 cut(s)	345, 351, 1467
SapI	GCTCTTC	1 cut(s)	641
SaqAI	TTAA	6 cut(s)	758, 1134, 1160, 1380, 1391, 1527
Sau3AI	GATC	7 cut(s)	299, 396, 437, 525, 639, 834, 1244
Sau96I	GGNCC	7 cut(s)	328, 375, 408, 431, 432, 487, 1353
SchI	GAGTC	2 cut(s)	1091, 1264
ScrFI	CCNGG	8 cut(s)	29, 369, 406, 435, 1154, 1303, 1357, 1532
SduI	GDGCHC	4 cut(s)	345, 351, 435, 1467
SfaNI	GCATC	6 cut(s)	56, 552, 640, 1337, 1350, 1396
SfcI	CTRYAG	1 cut(s)	1323
Sfr274I	CTCGAG	1 cut(s)	344
SinI	GGWCC	5 cut(s)	328, 375, 408, 487, 1353
SlaI	CTCGAG	1 cut(s)	344
SmiMI	CAYNNNNRTG	1 cut(s)	1505
SmlI	CTYRAG	3 cut(s)	311, 344, 1058
SmoI	CTYRAG	3 cut(s)	311, 344, 1058
SpeI	ACTAGT	1 cut(s)	985
SphI	GCATGC	2 cut(s)	727, 1510
SspI	AATATT	1 cut(s)	1338
SspMI	CTAG	3 cut(s)	986, 1140, 1217
SstI	GAGCTC	3 cut(s)	345, 351, 1467
StyD4I	CCNGG	8 cut(s)	27, 367, 404, 433, 1152, 1301, 1355, 1530
StyI	CCWWGG	1 cut(s)	810
TaaI	ACNGT	3 cut(s)	451, 1174, 1264
TaiI	ACGT	2 cut(s)	392, 474
TaqI	TCGA	8 cut(s)	78, 231, 345, 570, 729, 1144, 1467, 1476
TauI	GCSGC	3 cut(s)	599, 694, 819
TfiI	GAWTC	5 cut(s)	65, 233, 500, 980, 1440
Tru1I	TTAA	6 cut(s)	758, 1134, 1160, 1380, 1391, 1527
Tru9I	TTAA	6 cut(s)	758, 1134, 1160, 1380, 1391, 1527
TscAI	CASTG	1 cut(s)	744
TseFI	GTSAC	4 cut(s)	322, 475, 769, 778
Tsp45I	GTSAC	4 cut(s)	322, 475, 769, 778
TspDTI	ATGAA	8 cut(s)	508, 692, 1124, 1184, 1212, 1266, 1449, 1489
TspGWI	ACGGA	5 cut(s)	118, 341, 747, 797, 1079
TspRI	CASTG	1 cut(s)	744
VpaK11BI	GGWCC	5 cut(s)	328, 375, 408, 487, 1353
XapI	RAATTY	3 cut(s)	305, 941, 1162
XceI	RCATGY	3 cut(s)	418, 727, 1510
XhoI	CTCGAG	1 cut(s)	344
XmiI	GTMKAC	1 cut(s)	132
XmnI	GAANNNNTTC	1 cut(s)	504
XspI	CTAG	3 cut(s)	986, 1140, 1217

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.