Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr1

Orthogroup ID

OG0013716

Physical Location & Seq

Genomic Coordinates Forward (+)

16825197 .. 16826812

Gene Structure 1616 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom01g17580.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1314 bp

ATGATGAGGCAGGAAAACCACGGCGATCAGGAGGCCGACCTACATGCATCCCAAGATGACATGGAATCTCTCGTCCTTGACGAGCCACCCTCATCCAACGGCCAATCCTCGCCCTCCGCGTCCACCTCCGTGGACCGCCACTCCGCCACCGGAACAACGGCCACATCCTCGCAAGGCTTCAATTCCATCCTTGAACCCCCGTCTTACGCCGACGCCATCTTCACCTCGTTCGATTCCTCCTCATCCAACGGCCACGAAAGCCCTAGGCCCTCATCTTATCAATCGGAGCCTCCGACAAGATCCGAATTTCTCAAGATATGGGTCTCGGACCCTCAGAAGGAGCTCGAGCTCAACAATTCGCTCGTTCCCGGCGGGACCTCGTACTACACGTACCTGATCACCACCTGGACCAACATGCCCGAGTACGGCGGGCCCGGATCCGAATTCACCCTACGGAGGCGGTTCAAGGACGTGGTGACGCTGTCGGACCGGCTATCCGAATCGTTCCGTGGGTTCTTCATCCCGATCCGACCCGATAAGAGCGTGGTGGAGAGCCAGGTGATGCAAATCGAGCAGTTCGTGGAGCTGCGGAGGGCGGCACTGGCGAAGTACCTGAACAAGCTCGCGGTGCATCCGACGATCAAGAAGAGCGAGGAGCTGAGGCTGTTTTTGGTGGTGAAGGGGAAGCTACCGCTAACAAGAAGCATCGACATGGCGTCGCGGGTGCTCGATGGAGCAGTGAGGCTACCAAGGCAGCTGACGGGGGAGGTGGCTGTCGTGACAGATGTGAATGAGGCTGCGCAGCCGGCCAAGGGCGGCAGGGACTTGCTGAGGATCTTCAGGGAGATGAAGCAGGCGGTGGTGAACGATTGGGGCGGAGTGAAGCCGATGGCGGTTGAGGATGATAAAGAATTTTTGGAGAGGAAGGAGAAGGTGGCCGAGTTTGAGCAGCAGCTTAGCAATTTGTCTCAGCAGGCTGAATCACTAGTAAAAGCTCAGCAGGACATGGGAGAGACTCTGGGGGAATTGGGGTTGGCATTTGTGAAGCTGACCAAGCTTGAGACGGAGGGAGCCATGTTCGAACCTCAAACAGTACGAGCCAATGACATGAAAAATGTGGCTACTGCTTCTGTTAAAGCAAGCCGATTGTACCGGGAGTTAAATGCGCAAACGGTCAAACATTTGGACAAACTCCATGAATATCTTGGAGTAATGCTAGCAGTCAATAGTGCATTTTCAGACAGATCAAGCTCTTGCTTTACTGACGGTTCAGACTCTTTCATCGGAATTACTTTCGTTGCAGTCCAGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005794 GO:0005802 GO:0005829 GO:0006807 GO:0006810 GO:0006897 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009791 GO:0009987 GO:0010008 GO:0010467 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019538 GO:0019898 GO:0030904 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032585 GO:0032588 GO:0032991 GO:0035091 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0048856 GO:0051179 GO:0051234 GO:0051604 GO:0071704 GO:0071840 GO:0090351 GO:0097708 GO:0098588 GO:0098657 GO:0098791 GO:0098796 GO:0098805 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

PX Vps5

Protein Analysis

438

Amino Acids

48.44

Weight (kDa)

5.21

Isoelectric Point (pI)

46.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PX	PF00787	146 - 225	4.3e-14	PX domain
Vps5	PF09325	298 - 417	9.1e-09	Vps5 C terminal like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013716)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g25340 FvH4_7g25340 FvH4_7g25340
malus_domestica	MD01G1159500.v1.1 MD07G1228000.v1.1
prunus_persica	Prupe.2G257500_v2.0.a1
pyrus_communis	pycom01g17580 pycom07g20640
rosa_chinensis	RchiOBHm_Chr1g0371251
rosa_laevigata	RLG00000026976
rosa_roxburghii	Rroxscaffold_4G00285840
rosa_rugosa	Rorug01G0362400 Rorug01G0362400
rosa_samantha	Rh1AG372500 Rh1BG336000 Rh1CG349400 Rh1DG367100
rosa_wichuraiana	Rw1G032760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	801, 1167
AccII	CGCG	3 cut(s)	119, 626, 721
AclWI	GGATC	5 cut(s)	294, 432, 445, 520, 842
AcoI	YGGCCR	5 cut(s)	100, 159, 250, 807, 936
AcsI	RAATTY	3 cut(s)	305, 443, 911
AcuI	CTGAAG	1 cut(s)	823
AcyI	GRCGYC	2 cut(s)	213, 716
AfaI	GTAC	6 cut(s)	383, 392, 425, 611, 1095, 1151
AfiI	CCNNNNNNNGG	4 cut(s)	337, 425, 456, 812
AflIII	ACRYGT	1 cut(s)	387
AgsI	TTSAA	3 cut(s)	181, 194, 466
AhdI	GACNNNNNGTC	1 cut(s)	715
AhlI	ACTAGT	1 cut(s)	985
AjiI	CACGTC	1 cut(s)	472
AjnI	CCWGG	3 cut(s)	404, 555, 1305
AleI	CACNNNNGTG	1 cut(s)	128
Alw21I	GWGCWC	3 cut(s)	345, 351, 729
Alw26I	GTCTC	4 cut(s)	328, 972, 1007, 1055
AlwI	GGATC	5 cut(s)	294, 432, 445, 520, 842
Ama87I	CYCGRG	2 cut(s)	344, 419
AoxI	GGCC	8 cut(s)	33, 100, 159, 250, 266, 431, 807, 936
ApaI	GGGCCC	1 cut(s)	435
ApeKI	GCWGC	6 cut(s)	586, 754, 797, 802, 949, 952
ApoI	RAATTY	3 cut(s)	305, 443, 911
AspA2I	CCTAGG	1 cut(s)	263
AspLEI	GCGC	2 cut(s)	802, 1168
AspS9I	GGNCC	8 cut(s)	133, 267, 328, 375, 408, 431, 432, 487
AsuC2I	CCSGG	3 cut(s)	369, 435, 1154
AsuHPI	GGTGA	7 cut(s)	214, 391, 439, 487, 571, 688, 874
AsuII	TTCGAA	1 cut(s)	1080
AsuNHI	GCTAGC	1 cut(s)	1216
AvaI	CYCGRG	2 cut(s)	344, 419
AvaII	GGWCC	5 cut(s)	133, 328, 375, 408, 487
AvrII	CCTAGG	1 cut(s)	263
BaeGI	GKGCMC	1 cut(s)	435
BamHI	GGATCC	1 cut(s)	437
BanII	GRGCYC	3 cut(s)	345, 351, 435
Bbv12I	GWGCWC	3 cut(s)	345, 351, 729
BbvCI	CCTCAGC	2 cut(s)	659, 830
BbvI	GCAGC	6 cut(s)	573, 766, 784, 814, 961, 964
BccI	CCATC	4 cut(s)	194, 224, 725, 883
BceAI	ACGGC	5 cut(s)	37, 115, 174, 265, 442
BcgI	CGANNNNNNTGC	2 cut(s)	26, 60
BciT130I	CCWGG	3 cut(s)	406, 557, 1307
BclI	TGATCA	1 cut(s)	396
BcnI	CCSGG	3 cut(s)	369, 435, 1154
BcoDI	GTCTC	4 cut(s)	328, 972, 1007, 1055
BcuI	ACTAGT	1 cut(s)	985
BfaI	CTAG	3 cut(s)	264, 986, 1217
BisI	GCNGC	8 cut(s)	587, 597, 755, 798, 803, 817, 950, 953
BlnI	CCTAGG	1 cut(s)	263
BlpI	GCTNAGC	2 cut(s)	956, 996
BlsI	GCNGC	8 cut(s)	588, 598, 756, 799, 804, 818, 951, 954
Bme1390I	CCNGG	6 cut(s)	369, 406, 435, 557, 1154, 1307
Bme18I	GGWCC	5 cut(s)	133, 328, 375, 408, 487
BmeRI	GACNNNNNGTC	1 cut(s)	715
BmeT110I	CYCGRG	2 cut(s)	344, 419
BmgBI	CACGTC	1 cut(s)	472
BmgT120I	GGNCC	8 cut(s)	133, 267, 328, 375, 408, 431, 432, 487
BmiI	GGNNCC	6 cut(s)	288, 330, 376, 433, 439, 1072
BmrFI	CCNGG	6 cut(s)	369, 406, 435, 557, 1154, 1307
BmsI	GCATC	4 cut(s)	56, 552, 640, 714
BmtI	GCTAGC	1 cut(s)	1220
BplI	GAGNNNNNCTC	2 cut(s)	74, 106
Bpu10I	CCTNAGC	2 cut(s)	659, 830
Bpu1102I	GCTNAGC	2 cut(s)	956, 996
Bpu14I	TTCGAA	1 cut(s)	1080
BpuEI	CTTGAG	2 cut(s)	296, 1079
BpuMI	CCSGG	3 cut(s)	369, 435, 1154
BsaAI	YACGTR	1 cut(s)	390
BsaBI	GATNNNNATC	1 cut(s)	566
BsaHI	GRCGYC	2 cut(s)	213, 716
BsaI	GGTCTC	1 cut(s)	328
BsaJI	CCNNGG	6 cut(s)	19, 129, 263, 508, 749, 810
BsaWI	WCCGGW	1 cut(s)	149
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	4 cut(s)	337, 425, 456, 812
Bse118I	RCCGGY	2 cut(s)	489, 805
Bse1I	ACTGG	1 cut(s)	606
Bse8I	GATNNNNATC	1 cut(s)	566
BseBI	CCWGG	3 cut(s)	406, 557, 1307
BseDI	CCNNGG	6 cut(s)	19, 129, 263, 508, 749, 810
BseGI	GGATG	8 cut(s)	47, 92, 164, 186, 242, 519, 631, 907
BseJI	GATNNNNATC	1 cut(s)	566
BseLI	CCNNNNNNNGG	4 cut(s)	337, 425, 456, 812
BseMII	CTCAG	5 cut(s)	347, 650, 821, 983, 1010
BseNI	ACTGG	1 cut(s)	606
BseRI	GAGGAG	2 cut(s)	229, 668
BseSI	GKGCMC	1 cut(s)	435
BseXI	GCAGC	6 cut(s)	573, 766, 784, 814, 961, 964
Bsh1236I	CGCG	3 cut(s)	119, 626, 721
BshFI	GGCC	8 cut(s)	35, 102, 161, 252, 268, 433, 809, 938
BsiHKAI	GWGCWC	3 cut(s)	345, 351, 729
BsiHKCI	CYCGRG	2 cut(s)	344, 419
BsiSI	CCGG	6 cut(s)	150, 369, 435, 490, 806, 1153
BslFI	GGGAC	2 cut(s)	388, 836
BslI	CCNNNNNNNGG	4 cut(s)	337, 425, 456, 812
BsmAI	GTCTC	4 cut(s)	328, 972, 1007, 1055
BsmBI	CGTCTC	1 cut(s)	1055
BsmFI	GGGAC	2 cut(s)	388, 836
BsnI	GGCC	8 cut(s)	35, 102, 161, 252, 268, 433, 809, 938
Bso31I	GGTCTC	1 cut(s)	328
BsoBI	CYCGRG	2 cut(s)	344, 419
Bsp119I	TTCGAA	1 cut(s)	1080
Bsp120I	GGGCCC	1 cut(s)	431
Bsp1286I	GDGCHC	4 cut(s)	345, 351, 435, 729
Bsp143I	GATC	8 cut(s)	25, 299, 396, 437, 525, 639, 834, 1244
Bsp1720I	GCTNAGC	2 cut(s)	956, 996
BspANI	GGCC	8 cut(s)	35, 102, 161, 252, 268, 433, 809, 938
BspCNI	CTCAG	5 cut(s)	346, 651, 822, 982, 1009
BspFNI	CGCG	3 cut(s)	119, 626, 721
BspLI	GGNNCC	6 cut(s)	288, 330, 376, 433, 439, 1072
BspOI	GCTAGC	1 cut(s)	1220
BspPI	GGATC	5 cut(s)	294, 432, 445, 520, 842
BspQI	GCTCTTC	1 cut(s)	641
BspT104I	TTCGAA	1 cut(s)	1080
BspTNI	GGTCTC	1 cut(s)	328
BsrFI	RCCGGY	2 cut(s)	489, 805
BsrI	ACTGG	1 cut(s)	606
BssAI	RCCGGY	2 cut(s)	489, 805
BssECI	CCNNGG	6 cut(s)	19, 129, 263, 508, 749, 810
BssMI	GATC	8 cut(s)	25, 299, 396, 437, 525, 639, 834, 1244
BssNI	GRCGYC	2 cut(s)	213, 716
BssT1I	CCWWGG	3 cut(s)	263, 749, 810
Bst2UI	CCWGG	3 cut(s)	406, 557, 1307
Bst4CI	ACNGT	3 cut(s)	1093, 1174, 1268
Bst6I	CTCTTC	1 cut(s)	641
BstACI	GRCGYC	2 cut(s)	213, 716
BstBAI	YACGTR	1 cut(s)	390
BstBI	TTCGAA	1 cut(s)	1080
BstC8I	GCNNGC	7 cut(s)	431, 624, 807, 855, 975, 1141, 1218
BstDEI	CTNAG	6 cut(s)	333, 659, 830, 956, 969, 996
BstDSI	CCRYGG	3 cut(s)	19, 129, 508
BstF5I	GGATG	8 cut(s)	47, 92, 164, 186, 242, 519, 631, 907
BstFNI	CGCG	3 cut(s)	119, 626, 721
BstHHI	GCGC	2 cut(s)	802, 1168
BstKTI	GATC	8 cut(s)	28, 302, 399, 440, 528, 642, 837, 1247
BstMAI	GTCTC	4 cut(s)	328, 972, 1007, 1055
BstMBI	GATC	8 cut(s)	25, 299, 396, 437, 525, 639, 834, 1244
BstMWI	GCNNNNNNNGC	7 cut(s)	258, 571, 602, 628, 751, 806, 1054
BstNI	CCWGG	3 cut(s)	406, 557, 1307
BstNSI	RCATGY	2 cut(s)	47, 418
BstSCI	CCNGG	6 cut(s)	367, 404, 433, 555, 1152, 1305
BstSLI	GKGCMC	1 cut(s)	435
BstUI	CGCG	3 cut(s)	119, 626, 721
BstV1I	GCAGC	6 cut(s)	573, 766, 784, 814, 961, 964
BstX2I	RGATCY	3 cut(s)	299, 437, 834
BstXI	CCANNNNNNTGG	1 cut(s)	130
BstYI	RGATCY	3 cut(s)	299, 437, 834
BsuRI	GGCC	8 cut(s)	35, 102, 161, 252, 268, 433, 809, 938
BtgI	CCRYGG	3 cut(s)	19, 129, 508
BtrI	CACGTC	1 cut(s)	472
BtsCI	GGATG	8 cut(s)	47, 92, 164, 186, 242, 519, 631, 907
BtsI	GCAGTG	1 cut(s)	744
BtsIMutI	CAGTG	2 cut(s)	599, 744
Cac8I	GCNNGC	7 cut(s)	431, 624, 807, 855, 975, 1141, 1218
CfoI	GCGC	2 cut(s)	802, 1168
Cfr10I	RCCGGY	2 cut(s)	489, 805
Cfr13I	GGNCC	8 cut(s)	133, 267, 328, 375, 408, 431, 432, 487
CpoI	CGGWCCG	1 cut(s)	487
CseI	GACGC	4 cut(s)	108, 221, 487, 705
Csp6I	GTAC	6 cut(s)	382, 391, 424, 610, 1094, 1150
CspI	CGGWCCG	1 cut(s)	487
CviAII	CATG	8 cut(s)	44, 61, 415, 712, 1006, 1075, 1108, 1196
CviQI	GTAC	6 cut(s)	382, 391, 424, 610, 1094, 1150
DdeI	CTNAG	6 cut(s)	333, 659, 830, 956, 969, 996
DpnI	GATC	8 cut(s)	27, 301, 398, 439, 527, 641, 836, 1246
DpnII	GATC	8 cut(s)	25, 299, 396, 437, 525, 639, 834, 1244
DriI	GACNNNNNGTC	1 cut(s)	715
EaeI	YGGCCR	5 cut(s)	100, 159, 250, 807, 936
Eam1104I	CTCTTC	1 cut(s)	641
Eam1105I	GACNNNNNGTC	1 cut(s)	715
EarI	CTCTTC	1 cut(s)	641
EciI	GGCGGA	2 cut(s)	133, 891
Ecl136II	GAGCTC	2 cut(s)	343, 349
Eco130I	CCWWGG	3 cut(s)	263, 749, 810
Eco24I	GRGCYC	3 cut(s)	345, 351, 435
Eco31I	GGTCTC	1 cut(s)	328
Eco47I	GGWCC	5 cut(s)	133, 328, 375, 408, 487
Eco53kI	GAGCTC	2 cut(s)	343, 349
Eco57I	CTGAAG	1 cut(s)	823
Eco88I	CYCGRG	2 cut(s)	344, 419
EcoICRI	GAGCTC	2 cut(s)	343, 349
EcoO109I	RGGNCCY	2 cut(s)	267, 375
EcoRI	GAATTC	1 cut(s)	443
EcoRII	CCWGG	3 cut(s)	404, 555, 1305
EcoT14I	CCWWGG	3 cut(s)	263, 749, 810
EcoT22I	ATGCAT	1 cut(s)	49
EcoT38I	GRGCYC	3 cut(s)	345, 351, 435
ErhI	CCWWGG	3 cut(s)	263, 749, 810
Esp3I	CGTCTC	1 cut(s)	1055
FaeI	CATG	8 cut(s)	47, 64, 418, 715, 1009, 1078, 1111, 1199
FaiI	YATR	9 cut(s)	45, 62, 319, 416, 713, 1007, 1076, 1109, 1197
FaqI	GGGAC	2 cut(s)	388, 836
FatI	CATG	8 cut(s)	43, 60, 414, 711, 1005, 1074, 1107, 1195
FauI	CCCGC	3 cut(s)	365, 422, 714
FbaI	TGATCA	1 cut(s)	396
Fnu4HI	GCNGC	8 cut(s)	587, 597, 755, 798, 803, 817, 950, 953
FokI	GGATG	8 cut(s)	34, 79, 151, 173, 229, 506, 618, 914
FriOI	GRGCYC	3 cut(s)	345, 351, 435
Fsp4HI	GCNGC	8 cut(s)	587, 597, 755, 798, 803, 817, 950, 953
FspBI	CTAG	3 cut(s)	264, 986, 1217
FspI	TGCGCA	2 cut(s)	801, 1167
GlaI	GCGC	2 cut(s)	801, 1167
GluI	GCNGC	8 cut(s)	587, 597, 755, 798, 803, 817, 950, 953
HaeIII	GGCC	8 cut(s)	35, 102, 161, 252, 268, 433, 809, 938
HapII	CCGG	6 cut(s)	150, 369, 435, 490, 806, 1153
HgaI	GACGC	4 cut(s)	108, 221, 487, 705
HhaI	GCGC	2 cut(s)	802, 1168
Hin1I	GRCGYC	2 cut(s)	213, 716
Hin1II	CATG	8 cut(s)	47, 64, 418, 715, 1009, 1078, 1111, 1199
Hin6I	GCGC	2 cut(s)	800, 1166
HinP1I	GCGC	2 cut(s)	800, 1166
HindIII	AAGCTT	1 cut(s)	1055
HinfI	GANTC	6 cut(s)	65, 233, 500, 980, 1015, 1274
HpaII	CCGG	6 cut(s)	150, 369, 435, 490, 806, 1153
HphI	GGTGA	7 cut(s)	214, 391, 439, 487, 571, 688, 874
Hpy166II	GTNNAC	3 cut(s)	123, 133, 865
Hpy188III	TCNNGA	5 cut(s)	29, 313, 523, 643, 778
Hpy8I	GTNNAC	3 cut(s)	123, 133, 865
Hpy99I	CGWCG	3 cut(s)	215, 640, 721
HpyAV	CCTTC	4 cut(s)	331, 673, 919, 925
HpyCH4III	ACNGT	3 cut(s)	1093, 1174, 1268
HpyCH4IV	ACGT	2 cut(s)	389, 471
HpyCH4V	TGCA	5 cut(s)	47, 565, 631, 1232, 1301
HpyF10VI	GCNNNNNNNGC	7 cut(s)	258, 571, 602, 628, 751, 806, 1054
HpyF3I	CTNAG	6 cut(s)	333, 659, 830, 956, 969, 996
HpySE526I	ACGT	2 cut(s)	389, 471
Hsp92I	GRCGYC	2 cut(s)	213, 716
Hsp92II	CATG	8 cut(s)	47, 64, 418, 715, 1009, 1078, 1111, 1199
HspAI	GCGC	2 cut(s)	800, 1166
KroI	GCCGGC	1 cut(s)	805
KroNI	GCCGGC	1 cut(s)	807
Ksp22I	TGATCA	1 cut(s)	396
Kzo9I	GATC	8 cut(s)	25, 299, 396, 437, 525, 639, 834, 1244
LguI	GCTCTTC	1 cut(s)	641
LmnI	GCTCC	6 cut(s)	286, 340, 583, 655, 734, 1070
Lsp1109I	GCAGC	6 cut(s)	573, 766, 784, 814, 961, 964
LweI	GCATC	4 cut(s)	56, 552, 640, 714
MaeI	CTAG	3 cut(s)	264, 986, 1217
MaeII	ACGT	2 cut(s)	389, 471
MaeIII	GTNAC	2 cut(s)	475, 778
MalI	GATC	8 cut(s)	27, 301, 398, 439, 527, 641, 836, 1246
MboI	GATC	8 cut(s)	25, 299, 396, 437, 525, 639, 834, 1244
MboII	GAAGA	4 cut(s)	211, 508, 658, 829
MflI	RGATCY	3 cut(s)	299, 437, 834
MhlI	GDGCHC	4 cut(s)	345, 351, 435, 729
MluCI	AATT	8 cut(s)	181, 305, 355, 443, 911, 961, 1025, 1287
MlyI	GAGTC	2 cut(s)	1009, 1268
MmeI	TCCRAC	6 cut(s)	120, 270, 317, 465, 553, 659
Mph1103I	ATGCAT	1 cut(s)	49
MroNI	GCCGGC	1 cut(s)	805
MseI	TTAA	2 cut(s)	1134, 1160
MslI	CAYNNNNRTG	2 cut(s)	128, 710
MspA1I	CMGCKG	1 cut(s)	757
MspI	CCGG	6 cut(s)	150, 369, 435, 490, 806, 1153
MspR9I	CCNGG	6 cut(s)	369, 406, 435, 557, 1154, 1307
MvaI	CCWGG	3 cut(s)	406, 557, 1307
MvnI	CGCG	3 cut(s)	119, 626, 721
MwoI	GCNNNNNNNGC	7 cut(s)	258, 571, 602, 628, 751, 806, 1054
NaeI	GCCGGC	1 cut(s)	807
NciI	CCSGG	3 cut(s)	369, 435, 1154
NdeII	GATC	8 cut(s)	25, 299, 396, 437, 525, 639, 834, 1244
NgoMIV	GCCGGC	1 cut(s)	805
NheI	GCTAGC	1 cut(s)	1216
NlaIII	CATG	8 cut(s)	47, 64, 418, 715, 1009, 1078, 1111, 1199
NlaIV	GGNNCC	6 cut(s)	288, 330, 376, 433, 439, 1072
NmeAIII	GCCGAG	1 cut(s)	964
NmuCI	GTSAC	2 cut(s)	475, 778
NsbI	TGCGCA	2 cut(s)	801, 1167
NsiI	ATGCAT	1 cut(s)	49
NspI	RCATGY	2 cut(s)	47, 418
NspV	TTCGAA	1 cut(s)	1080
OliI	CACNNNNGTG	1 cut(s)	128
PaeR7I	CTCGAG	1 cut(s)	344
PciSI	GCTCTTC	1 cut(s)	641
PcsI	WCGNNNNNNNCGW	5 cut(s)	78, 116, 290, 386, 576
PdiI	GCCGGC	1 cut(s)	807
PfeI	GAWTC	4 cut(s)	65, 233, 500, 980
PflFI	GACNNNGTC	1 cut(s)	481
PfoI	TCCNGGA	1 cut(s)	1305
PkrI	GCNGC	8 cut(s)	588, 598, 756, 799, 804, 818, 951, 954
PleI	GAGTC	2 cut(s)	1009, 1268
PpsI	GAGTC	2 cut(s)	1009, 1268
Ppu21I	YACGTR	1 cut(s)	390
PpuMI	RGGWCCY	1 cut(s)	375
Psp124BI	GAGCTC	2 cut(s)	345, 351
Psp5II	RGGWCCY	1 cut(s)	375
Psp6I	CCWGG	3 cut(s)	404, 555, 1305
PspGI	CCWGG	3 cut(s)	404, 555, 1305
PspN4I	GGNNCC	6 cut(s)	288, 330, 376, 433, 439, 1072
PspOMI	GGGCCC	1 cut(s)	431
PspPI	GGNCC	8 cut(s)	133, 267, 328, 375, 408, 431, 432, 487
PspPPI	RGGWCCY	1 cut(s)	375
PspXI	VCTCGAGB	1 cut(s)	344
PsuI	RGATCY	3 cut(s)	299, 437, 834
PsyI	GACNNNGTC	1 cut(s)	481
PvuII	CAGCTG	1 cut(s)	757
RsaI	GTAC	6 cut(s)	383, 392, 425, 611, 1095, 1151
RsaNI	GTAC	6 cut(s)	382, 391, 424, 610, 1094, 1150
RseI	CAYNNNNRTG	2 cut(s)	128, 710
Rsr2I	CGGWCCG	1 cut(s)	487
RsrII	CGGWCCG	1 cut(s)	487
SacI	GAGCTC	2 cut(s)	345, 351
SapI	GCTCTTC	1 cut(s)	641
SaqAI	TTAA	2 cut(s)	1134, 1160
SatI	GCNGC	8 cut(s)	587, 597, 755, 798, 803, 817, 950, 953
Sau3AI	GATC	8 cut(s)	25, 299, 396, 437, 525, 639, 834, 1244
Sau96I	GGNCC	8 cut(s)	133, 267, 328, 375, 408, 431, 432, 487
SchI	GAGTC	2 cut(s)	1009, 1268
ScrFI	CCNGG	6 cut(s)	369, 406, 435, 557, 1154, 1307
SduI	GDGCHC	4 cut(s)	345, 351, 435, 729
SfaNI	GCATC	4 cut(s)	56, 552, 640, 714
Sfr274I	CTCGAG	1 cut(s)	344
SfuI	TTCGAA	1 cut(s)	1080
SinI	GGWCC	5 cut(s)	133, 328, 375, 408, 487
SlaI	CTCGAG	1 cut(s)	344
SmiMI	CAYNNNNRTG	2 cut(s)	128, 710
SmlI	CTYRAG	3 cut(s)	311, 344, 1058
SmoI	CTYRAG	3 cut(s)	311, 344, 1058
SpeI	ACTAGT	1 cut(s)	985
Sse9I	AATT	8 cut(s)	181, 305, 355, 443, 911, 961, 1025, 1287
SspMI	CTAG	3 cut(s)	264, 986, 1217
SstI	GAGCTC	2 cut(s)	345, 351
StyD4I	CCNGG	6 cut(s)	367, 404, 433, 555, 1152, 1305
StyI	CCWWGG	3 cut(s)	263, 749, 810
TaaI	ACNGT	3 cut(s)	1093, 1174, 1268
TaiI	ACGT	2 cut(s)	392, 474
TaqI	TCGA	6 cut(s)	231, 345, 570, 708, 729, 1080
TasI	AATT	8 cut(s)	181, 305, 355, 443, 911, 961, 1025, 1287
TauI	GCSGC	2 cut(s)	599, 819
TfiI	GAWTC	4 cut(s)	65, 233, 500, 980
Tru1I	TTAA	2 cut(s)	1134, 1160
Tru9I	TTAA	2 cut(s)	1134, 1160
TscAI	CASTG	2 cut(s)	606, 744
TseFI	GTSAC	2 cut(s)	475, 778
TseI	GCWGC	6 cut(s)	586, 754, 797, 802, 949, 952
Tsp45I	GTSAC	2 cut(s)	475, 778
TspDTI	ATGAA	5 cut(s)	508, 863, 1124, 1212, 1270
TspGWI	ACGGA	4 cut(s)	118, 469, 497, 1079
TspRI	CASTG	2 cut(s)	606, 744
Tth111I	GACNNNGTC	1 cut(s)	481
VpaK11BI	GGWCC	5 cut(s)	133, 328, 375, 408, 487
XapI	RAATTY	3 cut(s)	305, 443, 911
XceI	RCATGY	2 cut(s)	47, 418
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1202
XhoI	CTCGAG	1 cut(s)	344
XmaJI	CCTAGG	1 cut(s)	263
XspI	CTAG	3 cut(s)	264, 986, 1217
Zsp2I	ATGCAT	1 cut(s)	49

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.